Rroxscaffold_7G00215620

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000427
Physical Location & Seq
Forward (+)
66217783 .. 66219906
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215620.1

Sequence Viewer

Length: 1536 bp
ATGGCTATCTATTTTCTCCTCTCGTTTTTGCTTCTAATTTGCATTCTATCATATGGCTCACAAAAACTCACAGCCAAAAGGCTCAAAATCACAATCGAAGAAGCTACTGTTGATGATCTCCAGCTTGCTTTCAGGCTCAAGACTCTGACCTCAAGGCAAGTTGTCCTGTTCTACCTGGACAAGATTCGAGAACTGAACAGAAACCTTAGGGGTGTCATAGAGGTGAACCCTGATGCCCTATCCCAAGCTGATATAGCTGACAAACAACGCAGAGAATATTGCAACCACATACTGCCTAAGCTTCATGGCATTCCTGTTCTGCTAAAGGATAACATAGCAACCAAGGACAAACTCAACACCACAGCTGGCTCGTTTGCCTTGTTAGGATCCATTGTGCCTCGAGATGCAGGTGTTGTTTCTAAGCTAAGGAGGTCTGGAGCTATCATTTTAGGGAAGGCTAGCTTGAGTGAATGGAGCAACTTTAGAACATCATGGGCACCCAACGGTTGGAGCGCCAGAGGTTCCCAAGGAAAGAATCCTTACAATTCATCGGTGGAAGTTTGTGGATCAAGTAGTGGACCTGCAATATCAGCAGCTGCTAATATGGTTTCTGTGACACTAGGGACGGAGACCGACGGCTCCATTATCTGTCCGGCTAGCTTTAACTCAGTAGTTGGCATCAAACCGACTGTTGGTCTCACTAGTCGAGCTGGAGTCATACCAATCAGTCCAAGACAGGACACAGTTGGGCCGATTTGTAGGACTGTAGCGGATGCTGTTCACGTCCTTGATATCATTGTTGGCATTGATAGGAATGACAATGCAACAAGTGAAGCATCAAAGTACATCCCAGTGGGAGGCTATGGACAGTTTCTCAAGCATAATGGCCTTGAAGGAAAGAGATTGGGGATAGTAAATATGCTATTTGATTCAATCAAGCACGATGTTTTTCTGGAACAAACTTTTGAGCAGCATTTTACCACACTGAGGTATAACAGATATGCCCATATTGAAGATGTTCTGGATTCTCATTCAAGTGGAGAATTTGATGCAATGTTAGCTGAATTCAAAATAGCCTTGAATGAGTACCTAAGTGACCTAGTAAAATCTCCAGTGAAGTCCTTAAGGGAGGTGATAGAGTTCAACAAGAATCATTCAAAGGAGGAAAAGATCAATGAATATGAGCAAGATGTCTTCGAATTAGCCGAGAAGACTAATGGAATGGGACCGAAAGAGCAGGAGGCATTGTCAAATTTAAAAAGACTATCTAAACAGGGGTTCAAGAAGTTGATGACCGATCATAGCCTAGATGCCTTGGTGGCTTATGCTAACAGTGCTGCTTCTATCCTATCGCAATTGGTGGTTTTCCAGGCATTGTTGGTGCCGGTTTTCCAGGATATCATAACACTAAAAAGTATCCATTGTTGTGCCGGCATCTGCTTTGGGGGACTCAGGGGTTCAGAGCCAAAGCTGATTGAGATCGCCTATGCCTTCGAGCAAGCAACTAAGATAAGGAGAAAACCTCCTCTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

511

Amino Acids

56.16

Weight (kDa)

8.6

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 54 - 441 2.5e-63 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 398
Acc36I ACCTGC 2 cut(s) 398, 589
AccB1I GGYRCC 2 cut(s) 496, 1381
AccB7I CCANNNNNTGG 1 cut(s) 507
AciI CCGC 1 cut(s) 770
AclWI GGATC 3 cut(s) 381, 394, 574
AcsI RAATTY 3 cut(s) 1043, 1064, 1252
AfaI GTAC 2 cut(s) 845, 1088
AfiI CCNNNNNNNGG 4 cut(s) 507, 692, 857, 987
AflII CTTAAG 1 cut(s) 1123
AgsI TTSAA 9 cut(s) 893, 933, 1013, 1035, 1069, 1081, 1144, 1158, 1282
AhdI GACNNNNNGTC 1 cut(s) 693
AhlI ACTAGT 1 cut(s) 701
AjiI CACGTC 1 cut(s) 784
AjnI CCWGG 3 cut(s) 174, 1368, 1392
Alw26I GTCTC 2 cut(s) 623, 701
AlwI GGATC 3 cut(s) 381, 394, 574
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 1 cut(s) 399
AoxI GGCC 2 cut(s) 749, 886
ApeKI GCWGC 4 cut(s) 593, 596, 970, 1337
ApoI RAATTY 3 cut(s) 1043, 1064, 1252
Asp700I GAANNNNTTC 1 cut(s) 1017
AspLEI GCGC 1 cut(s) 515
AspS9I GGNCC 3 cut(s) 578, 749, 1226
AsuHPI GGTGA 2 cut(s) 235, 1144
AsuII TTCGAA 1 cut(s) 1197
AsuNHI GCTAGC 2 cut(s) 458, 656
AvaI CYCGRG 1 cut(s) 399
AvaII GGWCC 2 cut(s) 578, 1226
AxyI CCTNAGG 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 499
BamHI GGATCC 1 cut(s) 386
BanI GGYRCC 2 cut(s) 496, 1381
BbsI GAAGAC 2 cut(s) 1186, 1217
BbvI GCAGC 4 cut(s) 583, 605, 982, 1324
BceAI ACGGC 1 cut(s) 652
BciT130I CCWGG 3 cut(s) 176, 1370, 1394
BciVI GTATCC 1 cut(s) 1427
BcoDI GTCTC 2 cut(s) 623, 701
BcuI ACTAGT 1 cut(s) 701
BfaI CTAG 7 cut(s) 459, 620, 657, 702, 1100, 1307, 1529
BfmI CTRYAG 1 cut(s) 765
BfoI RGCGCY 1 cut(s) 516
BfrI CTTAAG 1 cut(s) 1123
BfuAI ACCTGC 2 cut(s) 398, 589
BfuI GTATCC 1 cut(s) 1427
BglI GCCNNNNNGGC 1 cut(s) 1319
BisI GCNGC 4 cut(s) 594, 597, 971, 1338
BlsI GCNGC 4 cut(s) 595, 598, 972, 1339
Bme1390I CCNGG 3 cut(s) 176, 1370, 1394
Bme18I GGWCC 2 cut(s) 578, 1226
BmeRI GACNNNNNGTC 1 cut(s) 693
BmeT110I CYCGRG 1 cut(s) 399
BmgBI CACGTC 1 cut(s) 784
BmgT120I GGNCC 3 cut(s) 578, 749, 1226
BmiI GGNNCC 6 cut(s) 388, 498, 523, 640, 1227, 1383
BmrFI CCNGG 3 cut(s) 176, 1370, 1394
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 8 cut(s) 223, 394, 687, 763, 845, 1039, 1300, 1443
BmtI GCTAGC 2 cut(s) 462, 660
BmuI ACTGGG 1 cut(s) 845
BpiI GAAGAC 2 cut(s) 1186, 1217
BplI GAGNNNNNCTC 1 cut(s) 1507
BpmI CTGGAG 4 cut(s) 104, 456, 732, 1095
Bpu10I CCTNAGC 2 cut(s) 297, 425
Bpu14I TTCGAA 1 cut(s) 1197
BpuEI CTTGAG 4 cut(s) 122, 136, 484, 860
BsaBI GATNNNNATC 1 cut(s) 1478
BsaI GGTCTC 2 cut(s) 623, 701
BsaJI CCNNGG 3 cut(s) 342, 526, 1314
BsaXI ACNNNNNCTCC 2 cut(s) 1232, 1262
Bsc4I CCNNNNNNNGG 4 cut(s) 507, 692, 857, 987
Bse118I RCCGGY 2 cut(s) 1384, 1430
Bse1I ACTGG 2 cut(s) 851, 1112
Bse21I CCTNAGG 1 cut(s) 206
Bse3DI GCAATG 1 cut(s) 1059
Bse8I GATNNNNATC 1 cut(s) 1478
BseBI CCWGG 3 cut(s) 176, 1370, 1394
BseDI CCNNGG 3 cut(s) 342, 526, 1314
BseGI GGATG 2 cut(s) 778, 846
BseJI GATNNNNATC 1 cut(s) 1478
BseLI CCNNNNNNNGG 4 cut(s) 507, 692, 857, 987
BseMI GCAATG 1 cut(s) 1059
BseMII CTCAG 3 cut(s) 681, 977, 1465
BseNI ACTGG 2 cut(s) 851, 1112
BseRI GAGGAG 2 cut(s) 8, 1515
BseSI GKGCMC 1 cut(s) 499
BseXI GCAGC 4 cut(s) 583, 605, 982, 1324
BshFI GGCC 2 cut(s) 751, 888
BshNI GGYRCC 2 cut(s) 496, 1381
BsiHKCI CYCGRG 1 cut(s) 399
BsiSI CCGG 3 cut(s) 653, 1385, 1431
BslFI GGGAC 3 cut(s) 637, 1239, 1461
BslI CCNNNNNNNGG 4 cut(s) 507, 692, 857, 987
BsmAI GTCTC 2 cut(s) 623, 701
BsmFI GGGAC 3 cut(s) 637, 1239, 1461
BsmI GAATGC 2 cut(s) 42, 309
BsnI GGCC 2 cut(s) 751, 888
Bso31I GGTCTC 2 cut(s) 623, 701
BsoBI CYCGRG 1 cut(s) 399
Bsp119I TTCGAA 1 cut(s) 1197
Bsp1286I GDGCHC 1 cut(s) 499
Bsp143I GATC 6 cut(s) 115, 386, 566, 1170, 1297, 1479
BspACI CCGC 1 cut(s) 770
BspANI GGCC 2 cut(s) 751, 888
BspCNI CTCAG 3 cut(s) 680, 978, 1464
BspLI GGNNCC 6 cut(s) 388, 498, 523, 640, 1227, 1383
BspMI ACCTGC 2 cut(s) 398, 589
BspOI GCTAGC 2 cut(s) 462, 660
BspPI GGATC 3 cut(s) 381, 394, 574
BspT104I TTCGAA 1 cut(s) 1197
BspT107I GGYRCC 2 cut(s) 496, 1381
BspTI CTTAAG 1 cut(s) 1123
BspTNI GGTCTC 2 cut(s) 623, 701
BsrDI GCAATG 1 cut(s) 1059
BsrFI RCCGGY 2 cut(s) 1384, 1430
BsrI ACTGG 2 cut(s) 851, 1112
BssAI RCCGGY 2 cut(s) 1384, 1430
BssECI CCNNGG 3 cut(s) 342, 526, 1314
BssMI GATC 6 cut(s) 115, 386, 566, 1170, 1297, 1479
BssT1I CCWWGG 3 cut(s) 342, 526, 1314
Bst2UI CCWGG 3 cut(s) 176, 1370, 1394
Bst4CI ACNGT 7 cut(s) 109, 506, 691, 745, 766, 870, 1334
BstAFI CTTAAG 1 cut(s) 1123
BstBI TTCGAA 1 cut(s) 1197
BstC8I GCNNGC 6 cut(s) 126, 367, 460, 658, 1432, 1500
BstDEI CTNAG 9 cut(s) 206, 297, 420, 425, 667, 986, 1091, 1451, 1506
BstF5I GGATG 2 cut(s) 778, 846
BstH2I RGCGCY 1 cut(s) 516
BstHHI GCGC 1 cut(s) 515
BstKTI GATC 6 cut(s) 118, 389, 569, 1173, 1300, 1482
BstMAI GTCTC 2 cut(s) 623, 701
BstMBI GATC 6 cut(s) 115, 386, 566, 1170, 1297, 1479
BstMWI GCNNNNNNNGC 5 cut(s) 254, 590, 1058, 1319, 1334
BstNI CCWGG 3 cut(s) 176, 1370, 1394
BstSCI CCNGG 3 cut(s) 174, 1368, 1392
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 1 cut(s) 499
BstV1I GCAGC 4 cut(s) 583, 605, 982, 1324
BstV2I GAAGAC 2 cut(s) 1186, 1217
BstX2I RGATCY 1 cut(s) 386
BstYI RGATCY 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 206
BsuI GTATCC 1 cut(s) 1427
BsuRI GGCC 2 cut(s) 751, 888
BtrI CACGTC 1 cut(s) 784
BtsCI GGATG 2 cut(s) 778, 846
BtsIMutI CAGTG 4 cut(s) 858, 983, 1119, 1339
BveI ACCTGC 2 cut(s) 398, 589
Cac8I GCNNGC 6 cut(s) 126, 367, 460, 658, 1432, 1500
CaiI CAGNNNCTG 1 cut(s) 596
CfoI GCGC 1 cut(s) 515
Cfr10I RCCGGY 2 cut(s) 1384, 1430
Cfr13I GGNCC 3 cut(s) 578, 749, 1226
Csp6I GTAC 2 cut(s) 844, 1087
CviAII CATG 2 cut(s) 305, 492
CviQI GTAC 2 cut(s) 844, 1087
DdeI CTNAG 9 cut(s) 206, 297, 420, 425, 667, 986, 1091, 1451, 1506
DpnI GATC 6 cut(s) 117, 388, 568, 1172, 1299, 1481
DpnII GATC 6 cut(s) 115, 386, 566, 1170, 1297, 1479
DraI TTTAAA 1 cut(s) 1257
DriI GACNNNNNGTC 1 cut(s) 693
Eam1105I GACNNNNNGTC 1 cut(s) 693
Eco130I CCWWGG 3 cut(s) 342, 526, 1314
Eco31I GGTCTC 2 cut(s) 623, 701
Eco32I GATATC 2 cut(s) 793, 1399
Eco47I GGWCC 2 cut(s) 578, 1226
Eco81I CCTNAGG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 399
EcoRI GAATTC 1 cut(s) 1064
EcoRII CCWGG 3 cut(s) 174, 1368, 1392
EcoRV GATATC 2 cut(s) 793, 1399
EcoT14I CCWWGG 3 cut(s) 342, 526, 1314
ErhI CCWWGG 3 cut(s) 342, 526, 1314
FaeI CATG 2 cut(s) 308, 495
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 3 cut(s) 637, 1239, 1461
FatI CATG 2 cut(s) 304, 491
FauNDI CATATG 1 cut(s) 52
Fnu4HI GCNGC 4 cut(s) 594, 597, 971, 1338
FokI GGATG 2 cut(s) 785, 833
Fsp4HI GCNGC 4 cut(s) 594, 597, 971, 1338
FspBI CTAG 7 cut(s) 459, 620, 657, 702, 1100, 1307, 1529
GlaI GCGC 1 cut(s) 514
GluI GCNGC 4 cut(s) 594, 597, 971, 1338
GsuI CTGGAG 4 cut(s) 104, 456, 732, 1095
HaeII RGCGCY 1 cut(s) 516
HaeIII GGCC 2 cut(s) 751, 888
HapII CCGG 3 cut(s) 653, 1385, 1431
HhaI GCGC 1 cut(s) 515
Hin1II CATG 2 cut(s) 308, 495
Hin6I GCGC 1 cut(s) 513
HinP1I GCGC 1 cut(s) 513
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 8 cut(s) 142, 184, 535, 714, 929, 1025, 1150, 1449
HpaII CCGG 3 cut(s) 653, 1385, 1431
HphI GGTGA 2 cut(s) 235, 1144
Hpy166II GTNNAC 3 cut(s) 226, 578, 781
Hpy188I TCNGA 2 cut(s) 147, 1462
Hpy188III TCNNGA 7 cut(s) 139, 188, 401, 435, 953, 1022, 1282
Hpy8I GTNNAC 3 cut(s) 226, 578, 781
Hpy99I CGWCG 1 cut(s) 638
HpyAV CCTTC 3 cut(s) 448, 887, 1501
HpyCH4III ACNGT 7 cut(s) 109, 506, 691, 745, 766, 870, 1334
HpyCH4IV ACGT 1 cut(s) 783
HpyCH4V TGCA 6 cut(s) 42, 282, 407, 584, 824, 1052
HpyF10VI GCNNNNNNNGC 5 cut(s) 254, 590, 1058, 1319, 1334
HpyF3I CTNAG 9 cut(s) 206, 297, 420, 425, 667, 986, 1091, 1451, 1506
HpySE526I ACGT 1 cut(s) 783
Hsp92II CATG 2 cut(s) 308, 495
HspAI GCGC 1 cut(s) 513
KroI GCCGGC 1 cut(s) 1430
KroNI GCCGGC 1 cut(s) 1432
Kzo9I GATC 6 cut(s) 115, 386, 566, 1170, 1297, 1479
LmnI GCTCC 4 cut(s) 437, 474, 510, 644
Lsp1109I GCAGC 4 cut(s) 583, 605, 982, 1324
LweI GCATC 8 cut(s) 223, 394, 687, 763, 845, 1039, 1300, 1443
MaeI CTAG 7 cut(s) 459, 620, 657, 702, 1100, 1307, 1529
MaeII ACGT 1 cut(s) 783
MaeIII GTNAC 2 cut(s) 613, 1094
MalI GATC 6 cut(s) 117, 388, 568, 1172, 1299, 1481
MboI GATC 6 cut(s) 115, 386, 566, 1170, 1297, 1479
MboII GAAGA 4 cut(s) 110, 1025, 1186, 1222
MfeI CAATTG 1 cut(s) 1355
MflI RGATCY 1 cut(s) 386
MhlI GDGCHC 1 cut(s) 499
MluCI AATT 7 cut(s) 36, 544, 1043, 1064, 1199, 1252, 1355
MlyI GAGTC 3 cut(s) 136, 723, 1443
MmeI TCCRAC 1 cut(s) 488
MroNI GCCGGC 1 cut(s) 1430
MroXI GAANNNNTTC 1 cut(s) 1017
MseI TTAA 3 cut(s) 663, 1124, 1256
MslI CAYNNNNRTG 4 cut(s) 221, 851, 1035, 1425
MspA1I CMGCKG 2 cut(s) 365, 596
MspCI CTTAAG 1 cut(s) 1123
MspI CCGG 3 cut(s) 653, 1385, 1431
MspR9I CCNGG 3 cut(s) 176, 1370, 1394
MunI CAATTG 1 cut(s) 1355
Mva1269I GAATGC 2 cut(s) 42, 309
MvaI CCWGG 3 cut(s) 176, 1370, 1394
MwoI GCNNNNNNNGC 5 cut(s) 254, 590, 1058, 1319, 1334
NaeI GCCGGC 1 cut(s) 1432
NdeI CATATG 1 cut(s) 52
NdeII GATC 6 cut(s) 115, 386, 566, 1170, 1297, 1479
NgoMIV GCCGGC 1 cut(s) 1430
NheI GCTAGC 2 cut(s) 458, 656
NlaIII CATG 2 cut(s) 308, 495
NlaIV GGNNCC 6 cut(s) 388, 498, 523, 640, 1227, 1383
NmeAIII GCCGAG 1 cut(s) 1231
NmuCI GTSAC 2 cut(s) 613, 1094
NspV TTCGAA 1 cut(s) 1197
PaeR7I CTCGAG 1 cut(s) 399
PaqCI CACCTGC 1 cut(s) 398
PcsI WCGNNNNNNNCGW 1 cut(s) 1203
PctI GAATGC 2 cut(s) 42, 309
PdiI GCCGGC 1 cut(s) 1432
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 5 cut(s) 184, 535, 929, 1025, 1150
PflMI CCANNNNNTGG 1 cut(s) 507
PfoI TCCNGGA 1 cut(s) 1392
PkrI GCNGC 4 cut(s) 595, 598, 972, 1339
PleI GAGTC 3 cut(s) 136, 722, 1443
PpsI GAGTC 3 cut(s) 136, 722, 1443
Psp6I CCWGG 3 cut(s) 174, 1368, 1392
PspGI CCWGG 3 cut(s) 174, 1368, 1392
PspN4I GGNNCC 6 cut(s) 388, 498, 523, 640, 1227, 1383
PspPI GGNCC 3 cut(s) 578, 749, 1226
PstNI CAGNNNCTG 1 cut(s) 596
PsuI RGATCY 1 cut(s) 386
PvuII CAGCTG 2 cut(s) 365, 596
RsaI GTAC 2 cut(s) 845, 1088
RsaNI GTAC 2 cut(s) 844, 1087
RseI CAYNNNNRTG 4 cut(s) 221, 851, 1035, 1425
SaqAI TTAA 3 cut(s) 663, 1124, 1256
SatI GCNGC 4 cut(s) 594, 597, 971, 1338
Sau3AI GATC 6 cut(s) 115, 386, 566, 1170, 1297, 1479
Sau96I GGNCC 3 cut(s) 578, 749, 1226
SchI GAGTC 3 cut(s) 136, 723, 1443
ScrFI CCNGG 3 cut(s) 176, 1370, 1394
SduI GDGCHC 1 cut(s) 499
SfaNI GCATC 8 cut(s) 223, 394, 687, 763, 845, 1039, 1300, 1443
SfcI CTRYAG 1 cut(s) 765
Sfr274I CTCGAG 1 cut(s) 399
SfuI TTCGAA 1 cut(s) 1197
SinI GGWCC 2 cut(s) 578, 1226
SlaI CTCGAG 1 cut(s) 399
SmiMI CAYNNNNRTG 4 cut(s) 221, 851, 1035, 1425
SmlI CTYRAG 6 cut(s) 137, 151, 399, 463, 875, 1123
SmoI CTYRAG 6 cut(s) 137, 151, 399, 463, 875, 1123
SpeI ACTAGT 1 cut(s) 701
Sse9I AATT 7 cut(s) 36, 544, 1043, 1064, 1199, 1252, 1355
SsiI CCGC 1 cut(s) 770
SspI AATATT 1 cut(s) 278
SspMI CTAG 7 cut(s) 459, 620, 657, 702, 1100, 1307, 1529
StyD4I CCNGG 3 cut(s) 174, 1368, 1392
StyI CCWWGG 3 cut(s) 342, 526, 1314
TaaI ACNGT 7 cut(s) 109, 506, 691, 745, 766, 870, 1334
TaiI ACGT 1 cut(s) 786
TaqI TCGA 6 cut(s) 96, 187, 400, 706, 1197, 1494
TaqII GACCGA 3 cut(s) 647, 1243, 1310
TasI AATT 7 cut(s) 36, 544, 1043, 1064, 1199, 1252, 1355
TatI WGTACW 1 cut(s) 843
TfiI GAWTC 5 cut(s) 184, 535, 929, 1025, 1150
Tru1I TTAA 3 cut(s) 663, 1124, 1256
Tru9I TTAA 3 cut(s) 663, 1124, 1256
TscAI CASTG 4 cut(s) 858, 990, 1119, 1339
TseFI GTSAC 2 cut(s) 613, 1094
TseI GCWGC 4 cut(s) 593, 596, 970, 1337
Tsp45I GTSAC 2 cut(s) 613, 1094
TspDTI ATGAA 3 cut(s) 293, 537, 1191
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 4 cut(s) 858, 990, 1119, 1339
Van91I CCANNNNNTGG 1 cut(s) 507
Vha464I CTTAAG 1 cut(s) 1123
VpaK11BI GGWCC 2 cut(s) 578, 1226
XapI RAATTY 3 cut(s) 1043, 1064, 1252
XhoI CTCGAG 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 1017
XspI CTAG 7 cut(s) 459, 620, 657, 702, 1100, 1307, 1529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.