RchiOBHm_Chr6g0244741

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000427
Physical Location & Seq
Reverse (-)
738874 .. 741769
2896 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21933

Sequence Viewer

Length: 1578 bp
ATGATAATTTCCTCATCGACTGCCATGGCAAAACCAAGCTTCCCTCACATCTTATCCTTATTGCTTCTCATTCTTCTCTTCACATTTTCATCTCTGCCAGGCACATTCGCAGCGCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTTAAGCACAACCAACTCACCTCGAGGCAGCTTGTCCAATATTACCATTCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAACCCGGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCTACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCACTGCTTGGATCTGTCGTGCCTCGTGATGCTGGCGTGGTGGAGAAGTTGAGGAAAGCTGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGTCTAATAAAGCACCCTCCGGTTGGAACGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCTGCAAATCTGGCAGCGGTTTCATTAGGTACTGAGACAGATGGTTCAATCCTATGTCCATCAGGTTATAACTCAGTTGTGGGCATCAAACCAACGATCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCTAAGGCAGGATACTGTCGGGCCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACAATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCAACGGACTTGGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATAACTTTGGCAATGACACTGCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTGGAAATTGCTAATTTGACTGATATCTATTCTTCAAGCGATGAAGATACTGCATTGTCTGTTGAGTGCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCAGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACCTATTTTTAGCAGCTGAAGCAACAAATGGGTCTGGAAAAACAGAGAAGCAGGCACTGTTGAATCTTGCAAGGTGGACAAGAATTGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGCTGTAGTGACTCCTAAAGCAGATTTTTCAAAGATACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCTGGATACGAGAACAATGGGAGACCGGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATTGAGATTGCATATGCTTTTGAGCAAGCTACTAAGATTAGAAAGCCTCCTTCATTCAAAGGATTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

525

Amino Acids

56.34

Weight (kDa)

9.51

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 65 - 409 8.4e-68 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AbsI CCTCGAGG 1 cut(s) 184
AccB1I GGYRCC 1 cut(s) 313
AccB7I CCANNNNNTGG 1 cut(s) 1210
AccI GTMKAC 1 cut(s) 1041
AccII CGCG 1 cut(s) 115
AciI CCGC 5 cut(s) 399, 558, 597, 609, 644
AclWI GGATC 2 cut(s) 430, 1161
AcuI CTGAAG 1 cut(s) 1275
AfaI GTAC 2 cut(s) 658, 878
AfiI CCNNNNNNNGG 4 cut(s) 543, 725, 890, 1210
AgsI TTSAA 9 cut(s) 571, 675, 1083, 1141, 1192, 1300, 1389, 1561, 1570
AhlI ACTAGT 1 cut(s) 734
AjnI CCWGG 2 cut(s) 97, 1414
Alw26I GTCTC 3 cut(s) 656, 860, 1447
AlwI GGATC 2 cut(s) 430, 1161
AlwNI CAGNNNCTG 4 cut(s) 629, 809, 1210, 1294
Ama87I CYCGRG 1 cut(s) 184
AoxI GGCC 1 cut(s) 782
ApeKI GCWGC 7 cut(s) 110, 190, 606, 629, 641, 809, 1250
AspLEI GCGC 5 cut(s) 115, 117, 271, 303, 915
AspS9I GGNCC 2 cut(s) 306, 782
AsuC2I CCSGG 2 cut(s) 233, 263
AsuHPI GGTGA 3 cut(s) 172, 266, 1351
AsuNHI GCTAGC 1 cut(s) 494
AvaI CYCGRG 1 cut(s) 184
AvaII GGWCC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 1414
BauI CACGAG 1 cut(s) 435
BbvI GCAGC 7 cut(s) 122, 202, 616, 618, 653, 796, 1262
BccI CCATC 2 cut(s) 662, 694
BciT130I CCWGG 2 cut(s) 99, 1416
BciVI GTATCC 2 cut(s) 766, 1430
BcnI CCSGG 2 cut(s) 233, 263
BcoDI GTCTC 3 cut(s) 656, 860, 1447
BcuI ACTAGT 1 cut(s) 734
BfaI CTAG 4 cut(s) 251, 495, 735, 1355
BfmI CTRYAG 1 cut(s) 1362
BfuI GTATCC 2 cut(s) 766, 1430
BglI GCCNNNNNGGC 1 cut(s) 287
BisI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
BlsI GCNGC 8 cut(s) 112, 192, 599, 608, 631, 643, 811, 1252
Bme1390I CCNGG 4 cut(s) 99, 233, 263, 1416
Bme18I GGWCC 1 cut(s) 306
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 306, 782
BmiI GGNNCC 2 cut(s) 315, 1411
BmrFI CCNGG 4 cut(s) 99, 233, 263, 1416
BmrI ACTGGG 1 cut(s) 1154
BmsI GCATC 7 cut(s) 256, 430, 720, 796, 814, 846, 1348
BmtI GCTAGC 1 cut(s) 498
BmuI ACTGGG 1 cut(s) 1154
BpmI CTGGAG 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 520, 586
BpuMI CCSGG 2 cut(s) 233, 263
BsaI GGTCTC 1 cut(s) 1447
BsaJI CCNNGG 6 cut(s) 24, 309, 378, 981, 1170, 1414
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 2 cut(s) 1017, 1047
Bsc4I CCNNNNNNNGG 4 cut(s) 543, 725, 890, 1210
Bse118I RCCGGY 2 cut(s) 401, 1456
Bse1I ACTGG 3 cut(s) 317, 1160, 1215
Bse3DI GCAATG 3 cut(s) 369, 852, 976
BseBI CCWGG 2 cut(s) 99, 1416
BseDI CCNNGG 6 cut(s) 24, 309, 378, 981, 1170, 1414
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 4 cut(s) 543, 725, 890, 1210
BseMI GCAATG 3 cut(s) 369, 852, 976
BseMII CTCAG 5 cut(s) 600, 651, 714, 922, 1007
BseNI ACTGG 3 cut(s) 317, 1160, 1215
BsePI GCGCGC 1 cut(s) 113
BseXI GCAGC 7 cut(s) 122, 202, 616, 618, 653, 796, 1262
BseYI CCCAGC 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 115
Bsh1285I CGRYCG 1 cut(s) 726
BshFI GGCC 1 cut(s) 784
BshNI GGYRCC 1 cut(s) 313
BsiEI CGRYCG 1 cut(s) 726
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 5 cut(s) 233, 263, 402, 540, 1457
BslFI GGGAC 1 cut(s) 737
BslI CCNNNNNNNGG 4 cut(s) 543, 725, 890, 1210
BsmAI GTCTC 3 cut(s) 656, 860, 1447
BsmFI GGGAC 1 cut(s) 737
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 1 cut(s) 784
Bso31I GGTCTC 1 cut(s) 1447
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 1414
Bsp143I GATC 5 cut(s) 148, 422, 723, 1166, 1218
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 5 cut(s) 399, 558, 597, 609, 644
BspANI GGCC 1 cut(s) 784
BspCNI CTCAG 5 cut(s) 599, 652, 713, 921, 1008
BspFNI CGCG 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 315, 1411
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 2 cut(s) 430, 1161
BspT107I GGYRCC 1 cut(s) 313
BspTNI GGTCTC 1 cut(s) 1447
BsrDI GCAATG 3 cut(s) 369, 852, 976
BsrFI RCCGGY 2 cut(s) 401, 1456
BsrI ACTGG 3 cut(s) 317, 1160, 1215
BssAI RCCGGY 2 cut(s) 401, 1456
BssECI CCNNGG 6 cut(s) 24, 309, 378, 981, 1170, 1414
BssHII GCGCGC 1 cut(s) 113
BssMI GATC 5 cut(s) 148, 422, 723, 1166, 1218
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 99, 1416
Bst4CI ACNGT 4 cut(s) 778, 799, 903, 1296
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 809
BstC8I GCNNGC 8 cut(s) 115, 159, 403, 445, 496, 835, 1290, 1530
BstDEI CTNAG 8 cut(s) 333, 586, 660, 700, 764, 908, 1016, 1536
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 1 cut(s) 115
BstHHI GCGC 5 cut(s) 115, 117, 271, 303, 915
BstKTI GATC 5 cut(s) 151, 425, 726, 1169, 1221
BstMAI GTCTC 3 cut(s) 656, 860, 1447
BstMBI GATC 5 cut(s) 148, 422, 723, 1166, 1218
BstMCI CGRYCG 1 cut(s) 726
BstMWI GCNNNNNNNGC 7 cut(s) 287, 564, 603, 606, 638, 809, 1256
BstNI CCWGG 2 cut(s) 99, 1416
BstSCI CCNGG 4 cut(s) 97, 231, 261, 1414
BstSFI CTRYAG 1 cut(s) 1362
BstUI CGCG 1 cut(s) 115
BstV1I GCAGC 7 cut(s) 122, 202, 616, 618, 653, 796, 1262
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
BsuI GTATCC 2 cut(s) 766, 1430
BsuRI GGCC 1 cut(s) 784
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 1101
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 2 cut(s) 413, 975
BtsIMutI CAGTG 6 cut(s) 324, 413, 630, 975, 1167, 1292
Cac8I GCNNGC 8 cut(s) 115, 159, 403, 445, 496, 835, 1290, 1530
CaiI CAGNNNCTG 4 cut(s) 629, 809, 1210, 1294
CfoI GCGC 5 cut(s) 115, 117, 271, 303, 915
Cfr10I RCCGGY 2 cut(s) 401, 1456
Cfr13I GGNCC 2 cut(s) 306, 782
Csp6I GTAC 2 cut(s) 657, 877
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 2 cut(s) 25, 341
CviQI GTAC 2 cut(s) 657, 877
DdeI CTNAG 8 cut(s) 333, 586, 660, 700, 764, 908, 1016, 1536
DpnI GATC 5 cut(s) 150, 424, 725, 1168, 1220
DpnII GATC 5 cut(s) 148, 422, 723, 1166, 1218
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco130I CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
Eco24I GRGCYC 1 cut(s) 1414
Eco31I GGTCTC 1 cut(s) 1447
Eco32I GATATC 1 cut(s) 1072
Eco47I GGWCC 1 cut(s) 306
Eco57I CTGAAG 1 cut(s) 1275
Eco88I CYCGRG 1 cut(s) 184
EcoRII CCWGG 2 cut(s) 97, 1414
EcoRV GATATC 1 cut(s) 1072
EcoT14I CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
EcoT22I ATGCAT 1 cut(s) 1135
EcoT38I GRGCYC 1 cut(s) 1414
ErhI CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
FaeI CATG 2 cut(s) 28, 344
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 1 cut(s) 737
FatI CATG 2 cut(s) 24, 340
FauNDI CATATG 1 cut(s) 1516
FblI GTMKAC 1 cut(s) 1041
Fnu4HI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 1414
Fsp4HI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
FspBI CTAG 4 cut(s) 251, 495, 735, 1355
GlaI GCGC 5 cut(s) 114, 116, 270, 302, 914
GluI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
GsaI CCCAGC 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 1 cut(s) 784
HapII CCGG 5 cut(s) 233, 263, 402, 540, 1457
HhaI GCGC 5 cut(s) 115, 117, 271, 303, 915
Hin1II CATG 2 cut(s) 28, 344
Hin6I GCGC 5 cut(s) 113, 115, 269, 301, 913
HinP1I GCGC 5 cut(s) 113, 115, 269, 301, 913
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 5 cut(s) 120, 246, 1300, 1369, 1566
HpaII CCGG 5 cut(s) 233, 263, 402, 540, 1457
HphI GGTGA 3 cut(s) 172, 266, 1351
Hpy166II GTNNAC 4 cut(s) 259, 1042, 1314, 1478
Hpy188I TCNGA 4 cut(s) 221, 589, 1017, 1492
Hpy188III TCNNGA 3 cut(s) 437, 821, 1272
Hpy8I GTNNAC 4 cut(s) 259, 1042, 1314, 1478
HpyAV CCTTC 4 cut(s) 964, 1135, 1198, 1563
HpyCH4III ACNGT 4 cut(s) 778, 799, 903, 1296
HpyF10VI GCNNNNNNNGC 7 cut(s) 287, 564, 603, 606, 638, 809, 1256
HpyF3I CTNAG 8 cut(s) 333, 586, 660, 700, 764, 908, 1016, 1536
Hsp92II CATG 2 cut(s) 28, 344
HspAI GCGC 5 cut(s) 113, 115, 269, 301, 913
KroI GCCGGC 1 cut(s) 401
KroNI GCCGGC 1 cut(s) 403
Kzo9I GATC 5 cut(s) 148, 422, 723, 1166, 1218
LmnI GCTCC 1 cut(s) 1409
Lsp1109I GCAGC 7 cut(s) 122, 202, 616, 618, 653, 796, 1262
LweI GCATC 7 cut(s) 256, 430, 720, 796, 814, 846, 1348
MaeI CTAG 4 cut(s) 251, 495, 735, 1355
MaeIII GTNAC 4 cut(s) 321, 510, 1339, 1366
MalI GATC 5 cut(s) 150, 424, 725, 1168, 1220
MboI GATC 5 cut(s) 148, 422, 723, 1166, 1218
MboII GAAGA 5 cut(s) 65, 70, 1071, 1103, 1360
MfeI CAATTG 2 cut(s) 861, 1401
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 1414
MlyI GAGTC 3 cut(s) 114, 255, 1363
MmeI TCCRAC 1 cut(s) 524
Mph1103I ATGCAT 1 cut(s) 1135
MroNI GCCGGC 1 cut(s) 401
MseI TTAA 2 cut(s) 165, 1127
MspA1I CMGCKG 4 cut(s) 609, 644, 1253, 1433
MspI CCGG 5 cut(s) 233, 263, 402, 540, 1457
MspR9I CCNGG 4 cut(s) 99, 233, 263, 1416
MunI CAATTG 2 cut(s) 861, 1401
Mva1269I GAATGC 1 cut(s) 345
MvaI CCWGG 2 cut(s) 99, 1416
MvnI CGCG 1 cut(s) 115
MwoI GCNNNNNNNGC 7 cut(s) 287, 564, 603, 606, 638, 809, 1256
NaeI GCCGGC 1 cut(s) 403
NciI CCSGG 2 cut(s) 233, 263
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 1516
NdeII GATC 5 cut(s) 148, 422, 723, 1166, 1218
NgoMIV GCCGGC 1 cut(s) 401
NheI GCTAGC 1 cut(s) 494
NlaIII CATG 2 cut(s) 28, 344
NlaIV GGNNCC 2 cut(s) 315, 1411
NmuCI GTSAC 4 cut(s) 321, 510, 1339, 1366
NsiI ATGCAT 1 cut(s) 1135
PaeR7I CTCGAG 1 cut(s) 184
PauI GCGCGC 1 cut(s) 113
PctI GAATGC 1 cut(s) 345
PdiI GCCGGC 1 cut(s) 403
PfeI GAWTC 2 cut(s) 1300, 1566
PflMI CCANNNNNTGG 1 cut(s) 1210
PkrI GCNGC 8 cut(s) 112, 192, 599, 608, 631, 643, 811, 1252
Ple19I CGATCG 1 cut(s) 726
PleI GAGTC 3 cut(s) 114, 254, 1363
PpsI GAGTC 3 cut(s) 114, 254, 1363
PsiI TTATAA 1 cut(s) 696
Psp6I CCWGG 2 cut(s) 97, 1414
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 2 cut(s) 97, 1414
PspN4I GGNNCC 2 cut(s) 315, 1411
PspPI GGNCC 2 cut(s) 306, 782
PspXI VCTCGAGB 1 cut(s) 184
PstNI CAGNNNCTG 4 cut(s) 629, 809, 1210, 1294
PsuI RGATCY 1 cut(s) 422
PteI GCGCGC 1 cut(s) 113
PvuI CGATCG 1 cut(s) 726
PvuII CAGCTG 2 cut(s) 1253, 1433
RsaI GTAC 2 cut(s) 658, 878
RsaNI GTAC 2 cut(s) 657, 877
SaqAI TTAA 2 cut(s) 165, 1127
SatI GCNGC 8 cut(s) 111, 191, 598, 607, 630, 642, 810, 1251
Sau3AI GATC 5 cut(s) 148, 422, 723, 1166, 1218
Sau96I GGNCC 2 cut(s) 306, 782
SchI GAGTC 3 cut(s) 114, 255, 1363
ScrFI CCNGG 4 cut(s) 99, 233, 263, 1416
SduI GDGCHC 1 cut(s) 1414
SfaNI GCATC 7 cut(s) 256, 430, 720, 796, 814, 846, 1348
SfcI CTRYAG 1 cut(s) 1362
Sfr274I CTCGAG 1 cut(s) 184
SinI GGWCC 1 cut(s) 306
SlaI CTCGAG 1 cut(s) 184
SmlI CTYRAG 3 cut(s) 184, 499, 601
SmoI CTYRAG 3 cut(s) 184, 499, 601
SpeI ACTAGT 1 cut(s) 734
SsiI CCGC 5 cut(s) 399, 558, 597, 609, 644
SspI AATATT 1 cut(s) 203
SspMI CTAG 4 cut(s) 251, 495, 735, 1355
StyD4I CCNGG 4 cut(s) 97, 231, 261, 1414
StyI CCWWGG 5 cut(s) 24, 309, 378, 981, 1170
TaaI ACNGT 4 cut(s) 778, 799, 903, 1296
TaqI TCGA 4 cut(s) 17, 185, 739, 840
TaqII GACCGA 1 cut(s) 716
TatI WGTACW 1 cut(s) 876
TauI GCSGC 1 cut(s) 600
TfiI GAWTC 2 cut(s) 1300, 1566
Tru1I TTAA 2 cut(s) 165, 1127
Tru9I TTAA 2 cut(s) 165, 1127
TscAI CASTG 6 cut(s) 324, 420, 630, 982, 1167, 1299
TseFI GTSAC 4 cut(s) 321, 510, 1339, 1366
TseI GCWGC 7 cut(s) 110, 190, 606, 629, 641, 809, 1250
Tsp45I GTSAC 4 cut(s) 321, 510, 1339, 1366
TspDTI ATGAA 5 cut(s) 78, 404, 639, 1104, 1545
TspGWI ACGGA 1 cut(s) 933
TspRI CASTG 6 cut(s) 324, 420, 630, 982, 1167, 1299
Van91I CCANNNNNTGG 1 cut(s) 1210
VpaK11BI GGWCC 1 cut(s) 306
XhoI CTCGAG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 1041
XspI CTAG 4 cut(s) 251, 495, 735, 1355
Zsp2I ATGCAT 1 cut(s) 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.