RLG00000015334

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000427
Physical Location & Seq
Forward (+)
64725426 .. 64727857
2432 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015334

Sequence Viewer

Length: 1416 bp
ATGAGTGCGGACCTGATCGAGGGTCTTTCCAAACCTGCCTGGAATAGGTGCTCTCTGGGATCAAGTGTGTATAAGTTGCCATACATGCTTGTCCAAATCCGAAAACTGAACCCGGGTCTCAAAGGAGTCCTAGAAGTCAACCCAGATGCGCTTCACCTTACCGATAAGGTTGACTACGAGCGCAGGACTAAGGCACCAGTGTCACTACTCTCTAAGTTGCATCGCATTCCTATTCTGGTCAAGGACAACATTGCAACTAAGGATAAGATGAACACCACCGCCGGATCTTTTGCACTGCTTGGATCTGTCGTGCCTCGTGATACTGGCGTGGTGGAGAAGTTGAGGAAAGCAGGGGCAATCATTTTGGGAAAGGCAAGCTTGAGCGAGTGGTCATATTTTAGGTGTAGTAAAGCACCCAATGGTTGGAGCGCCAGAGGCGGTCATGGCTTGAACCCTTACACATTCTCAGACCCTTGTGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTGTCATTAGGTACTGAGACAGATGGTTCAATCTTATGTCCATCAGATTTCAACGCGGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGTGCAGGGGTTGTCCCAATTTCTCCAAGACAGGAGACTGTTGGGACAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGAGGAAAAAGAATAGGGATATTGATAGCCTTCTATGACTTTGGCTATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCAATATTCTGCAGGCCGGAAAGGAGCTAGGTGCTGTTTTGGTATACAATTTGAAAATTGCTAATTTGACTGAAATCTATTCCTCAAACTATGAATATAATGCATTGTATATTGAGTTCAAAACATGCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCGGAAAAGATCAAGAAGTATGGGCAAGACCTATTTTTAGCTGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCCAGCATTGTTGAATCTTGCAAGGTGGACAAGATATGGTCTTGTGAAATTAATGACAGAGAAGAAGCTAGATGCTGTGGTGACTCCTAAATCAGATTTTCGAACTGTACTTGCAATTGGGGGAGCCCCAGGTCTAACAGTTCCAGCTGGATACGAAAACAATGGGAGACCAGTTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCCACTAAGATTAGGAAGCCTCCTTTATTTAAAGGCTTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

472

Amino Acids

50.96

Weight (kDa)

9.38

Isoelectric Point (pI)

32.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 28 - 352 8.7e-60 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 193
AccB7I CCANNNNNTGG 1 cut(s) 423
AccI GTMKAC 1 cut(s) 930
AccII CGCG 2 cut(s) 581, 795
AciI CCGC 6 cut(s) 8, 279, 438, 489, 524, 581
AclWI GGATC 3 cut(s) 67, 292, 310
AcuI CTGAAG 1 cut(s) 1113
AfaI GTAC 3 cut(s) 538, 758, 1233
AfiI CCNNNNNNNGG 5 cut(s) 19, 45, 423, 605, 770
AgsI TTSAA 8 cut(s) 451, 555, 577, 940, 1006, 1030, 1138, 1408
AhlI ACTAGT 1 cut(s) 614
AjnI CCWGG 2 cut(s) 38, 1252
AluBI AGCT 6 cut(s) 378, 913, 1088, 1192, 1271, 1339
AluI AGCT 6 cut(s) 378, 913, 1088, 1192, 1271, 1339
Alw21I GWGCWC 1 cut(s) 53
Alw26I GTCTC 5 cut(s) 122, 536, 647, 740, 1285
AlwI GGATC 3 cut(s) 67, 292, 310
AlwNI CAGNNNCTG 1 cut(s) 689
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 1 cut(s) 900
ApeKI GCWGC 5 cut(s) 486, 509, 521, 689, 1088
AseI ATTAAT 1 cut(s) 1175
AspLEI GCGC 4 cut(s) 151, 183, 431, 795
AspS9I GGNCC 1 cut(s) 10
AsuC2I CCSGG 3 cut(s) 113, 114, 1049
AsuHPI GGTGA 2 cut(s) 146, 1216
AsuII TTCGAA 1 cut(s) 1225
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 1 cut(s) 10
BaeI ACNNNNGTAYC 2 cut(s) 312, 345
BanI GGYRCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 1252
BauI CACGAG 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 53
BbvI GCAGC 5 cut(s) 498, 521, 533, 676, 1075
BccI CCATC 2 cut(s) 542, 574
BciT130I CCWGG 2 cut(s) 40, 1254
BciVI GTATCC 1 cut(s) 1268
BcnI CCSGG 3 cut(s) 113, 114, 1049
BcoDI GTCTC 5 cut(s) 122, 536, 647, 740, 1285
BcuI ACTAGT 1 cut(s) 614
BfaI CTAG 4 cut(s) 131, 615, 914, 1193
BfmI CTRYAG 1 cut(s) 896
BfoI RGCGCY 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 43
BfuI GTATCC 1 cut(s) 1268
BisI GCNGC 5 cut(s) 487, 510, 522, 690, 1089
BlsI GCNGC 5 cut(s) 488, 511, 523, 691, 1090
Bme1390I CCNGG 5 cut(s) 40, 113, 114, 1049, 1254
Bme18I GGWCC 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 2 cut(s) 195, 1249
BmrFI CCNGG 5 cut(s) 40, 113, 114, 1049, 1254
BmsI GCATC 7 cut(s) 136, 229, 600, 676, 694, 726, 1186
Bpu14I TTCGAA 1 cut(s) 1225
BpuEI CTTGAG 2 cut(s) 400, 466
BpuMI CCSGG 3 cut(s) 113, 114, 1049
BsaI GGTCTC 2 cut(s) 122, 1285
BsaJI CCNNGG 4 cut(s) 112, 861, 1047, 1252
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 45, 423, 605, 770
Bse1I ACTGG 3 cut(s) 197, 328, 1295
Bse3DI GCAATG 2 cut(s) 249, 732
BseBI CCWGG 2 cut(s) 40, 1254
BseDI CCNNGG 4 cut(s) 112, 861, 1047, 1252
BseLI CCNNNNNNNGG 5 cut(s) 19, 45, 423, 605, 770
BseMI GCAATG 2 cut(s) 249, 732
BseMII CTCAG 3 cut(s) 480, 531, 802
BseNI ACTGG 3 cut(s) 197, 328, 1295
BseXI GCAGC 5 cut(s) 498, 521, 533, 676, 1075
BsgI GTGCAG 1 cut(s) 642
Bsh1236I CGCG 2 cut(s) 581, 795
BshFI GGCC 1 cut(s) 902
BshNI GGYRCC 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 53
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 4 cut(s) 113, 282, 903, 1049
BslFI GGGAC 2 cut(s) 617, 676
BslI CCNNNNNNNGG 5 cut(s) 19, 45, 423, 605, 770
BsmAI GTCTC 5 cut(s) 122, 536, 647, 740, 1285
BsmFI GGGAC 2 cut(s) 617, 676
BsmI GAATGC 1 cut(s) 225
BsnI GGCC 1 cut(s) 902
Bso31I GGTCTC 2 cut(s) 122, 1285
BsoBI CYCGRG 1 cut(s) 112
Bsp119I TTCGAA 1 cut(s) 1225
Bsp1286I GDGCHC 2 cut(s) 53, 1252
Bsp143I GATC 5 cut(s) 15, 59, 284, 302, 1056
BspACI CCGC 6 cut(s) 8, 279, 438, 489, 524, 581
BspANI GGCC 1 cut(s) 902
BspCNI CTCAG 3 cut(s) 479, 532, 801
BspFNI CGCG 2 cut(s) 581, 795
BspLI GGNNCC 2 cut(s) 195, 1249
BspMAI CTGCAG 1 cut(s) 900
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 3 cut(s) 67, 292, 310
BspT104I TTCGAA 1 cut(s) 1225
BspT107I GGYRCC 1 cut(s) 193
BspTNI GGTCTC 2 cut(s) 122, 1285
BsrDI GCAATG 2 cut(s) 249, 732
BsrI ACTGG 3 cut(s) 197, 328, 1295
BssECI CCNNGG 4 cut(s) 112, 861, 1047, 1252
BssMI GATC 5 cut(s) 15, 59, 284, 302, 1056
BssNAI GTATAC 1 cut(s) 931
BssSI CACGAG 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 861
Bst1107I GTATAC 1 cut(s) 931
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 40, 1254
Bst4CI ACNGT 5 cut(s) 658, 679, 783, 1231, 1264
BstAPI GCANNNNNTGC 1 cut(s) 689
BstBI TTCGAA 1 cut(s) 1225
BstC8I GCNNGC 5 cut(s) 376, 715, 900, 1128, 1368
BstDEI CTNAG 8 cut(s) 189, 213, 258, 466, 540, 788, 803, 1374
BstENI CCTNNNNNAGG 2 cut(s) 17, 43
BstFNI CGCG 2 cut(s) 581, 795
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 4 cut(s) 151, 183, 431, 795
BstKTI GATC 5 cut(s) 18, 62, 287, 305, 1059
BstMAI GTCTC 5 cut(s) 122, 536, 647, 740, 1285
BstMBI GATC 5 cut(s) 15, 59, 284, 302, 1056
BstMWI GCNNNNNNNGC 7 cut(s) 85, 435, 444, 486, 518, 689, 1094
BstNI CCWGG 2 cut(s) 40, 1254
BstNSI RCATGY 2 cut(s) 88, 1014
BstSCI CCNGG 5 cut(s) 38, 111, 112, 1047, 1252
BstSFI CTRYAG 1 cut(s) 896
BstUI CGCG 2 cut(s) 581, 795
BstV1I GCAGC 5 cut(s) 498, 521, 533, 676, 1075
BstX2I RGATCY 2 cut(s) 284, 302
BstYI RGATCY 2 cut(s) 284, 302
BstZ17I GTATAC 1 cut(s) 931
BsuI GTATCC 1 cut(s) 1268
BsuRI GGCC 1 cut(s) 902
BtgZI GCGATG 1 cut(s) 206
BtsI GCAGTG 1 cut(s) 293
BtsIMutI CAGTG 3 cut(s) 204, 293, 510
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 5 cut(s) 376, 715, 900, 1128, 1368
CaiI CAGNNNCTG 1 cut(s) 689
CfoI GCGC 4 cut(s) 151, 183, 431, 795
Cfr13I GGNCC 1 cut(s) 10
Cfr9I CCCGGG 1 cut(s) 112
Csp6I GTAC 3 cut(s) 537, 757, 1232
CspCI CAANNNNNGTGG 2 cut(s) 486, 521
CviAII CATG 3 cut(s) 85, 443, 1011
CviQI GTAC 3 cut(s) 537, 757, 1232
DdeI CTNAG 8 cut(s) 189, 213, 258, 466, 540, 788, 803, 1374
DpnI GATC 5 cut(s) 17, 61, 286, 304, 1058
DpnII GATC 5 cut(s) 15, 59, 284, 302, 1056
DraI TTTAAA 1 cut(s) 1399
Eco130I CCWWGG 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 1252
Eco31I GGTCTC 2 cut(s) 122, 1285
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 1113
Eco88I CYCGRG 1 cut(s) 112
EcoNI CCTNNNNNAGG 2 cut(s) 17, 43
EcoRII CCWGG 2 cut(s) 38, 1252
EcoT14I CCWWGG 1 cut(s) 861
EcoT22I ATGCAT 2 cut(s) 991, 1024
EcoT38I GRGCYC 1 cut(s) 1252
ErhI CCWWGG 1 cut(s) 861
FaeI CATG 3 cut(s) 88, 446, 1014
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 2 cut(s) 617, 676
FatI CATG 3 cut(s) 84, 442, 1010
FauNDI CATATG 1 cut(s) 1354
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 5 cut(s) 487, 510, 522, 690, 1089
FriOI GRGCYC 1 cut(s) 1252
Fsp4HI GCNGC 5 cut(s) 487, 510, 522, 690, 1089
FspBI CTAG 4 cut(s) 131, 615, 914, 1193
GlaI GCGC 4 cut(s) 150, 182, 430, 794
GluI GCNGC 5 cut(s) 487, 510, 522, 690, 1089
HaeII RGCGCY 1 cut(s) 432
HaeIII GGCC 1 cut(s) 902
HapII CCGG 4 cut(s) 113, 282, 903, 1049
HhaI GCGC 4 cut(s) 151, 183, 431, 795
Hin1II CATG 3 cut(s) 88, 446, 1014
Hin6I GCGC 4 cut(s) 149, 181, 429, 793
HinP1I GCGC 4 cut(s) 149, 181, 429, 793
HincII GTYRAC 2 cut(s) 139, 172
HindII GTYRAC 2 cut(s) 139, 172
HindIII AAGCTT 1 cut(s) 376
HinfI GANTC 3 cut(s) 126, 1138, 1207
HpaII CCGG 4 cut(s) 113, 282, 903, 1049
HphI GGTGA 2 cut(s) 146, 1216
Hpy166II GTNNAC 5 cut(s) 139, 172, 931, 1152, 1316
Hpy188I TCNGA 5 cut(s) 101, 469, 571, 1219, 1330
Hpy188III TCNNGA 4 cut(s) 317, 701, 1060, 1345
Hpy8I GTNNAC 5 cut(s) 139, 172, 931, 1152, 1316
Hpy99I CGWCG 1 cut(s) 800
HpyAV CCTTC 2 cut(s) 844, 1024
HpyCH4III ACNGT 5 cut(s) 658, 679, 783, 1231, 1264
HpyF10VI GCNNNNNNNGC 7 cut(s) 85, 435, 444, 486, 518, 689, 1094
HpyF3I CTNAG 8 cut(s) 189, 213, 258, 466, 540, 788, 803, 1374
Hsp92II CATG 3 cut(s) 88, 446, 1014
HspAI GCGC 4 cut(s) 149, 181, 429, 793
Kzo9I GATC 5 cut(s) 15, 59, 284, 302, 1056
LmnI GCTCC 3 cut(s) 426, 910, 1247
Lsp1109I GCAGC 5 cut(s) 498, 521, 533, 676, 1075
LweI GCATC 7 cut(s) 136, 229, 600, 676, 694, 726, 1186
MaeI CTAG 4 cut(s) 131, 615, 914, 1193
MaeIII GTNAC 2 cut(s) 201, 1204
MalI GATC 5 cut(s) 17, 61, 286, 304, 1058
MboI GATC 5 cut(s) 15, 59, 284, 302, 1056
MboII GAAGA 1 cut(s) 1198
MfeI CAATTG 1 cut(s) 1239
MflI RGATCY 2 cut(s) 284, 302
MhlI GDGCHC 2 cut(s) 53, 1252
MluCI AATT 7 cut(s) 636, 666, 934, 942, 949, 1172, 1239
MlyI GAGTC 2 cut(s) 135, 1201
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 7 cut(s) 13, 324, 336, 428, 800, 979, 1398
Mph1103I ATGCAT 2 cut(s) 991, 1024
MseI TTAA 3 cut(s) 1016, 1175, 1398
MspA1I CMGCKG 3 cut(s) 489, 524, 1271
MspI CCGG 4 cut(s) 113, 282, 903, 1049
MspR9I CCNGG 5 cut(s) 40, 113, 114, 1049, 1254
MunI CAATTG 1 cut(s) 1239
Mva1269I GAATGC 1 cut(s) 225
MvaI CCWGG 2 cut(s) 40, 1254
MvnI CGCG 2 cut(s) 581, 795
MwoI GCNNNNNNNGC 7 cut(s) 85, 435, 444, 486, 518, 689, 1094
NciI CCSGG 3 cut(s) 113, 114, 1049
NdeI CATATG 1 cut(s) 1354
NdeII GATC 5 cut(s) 15, 59, 284, 302, 1056
NlaIII CATG 3 cut(s) 88, 446, 1014
NlaIV GGNNCC 2 cut(s) 195, 1249
NmuCI GTSAC 2 cut(s) 201, 1204
NsiI ATGCAT 2 cut(s) 991, 1024
NspI RCATGY 2 cut(s) 88, 1014
NspV TTCGAA 1 cut(s) 1225
PctI GAATGC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 1138
PflMI CCANNNNNTGG 1 cut(s) 423
PkrI GCNGC 5 cut(s) 488, 511, 523, 691, 1090
PleI GAGTC 2 cut(s) 134, 1201
PpsI GAGTC 2 cut(s) 134, 1201
PshBI ATTAAT 1 cut(s) 1175
Psp6I CCWGG 2 cut(s) 38, 1252
PspGI CCWGG 2 cut(s) 38, 1252
PspN4I GGNNCC 2 cut(s) 195, 1249
PspPI GGNCC 1 cut(s) 10
PsrI GAACNNNNNNTAC 2 cut(s) 986, 1018
PstI CTGCAG 1 cut(s) 900
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 284, 302
PvuII CAGCTG 1 cut(s) 1271
RsaI GTAC 3 cut(s) 538, 758, 1233
RsaNI GTAC 3 cut(s) 537, 757, 1232
SaqAI TTAA 3 cut(s) 1016, 1175, 1398
SatI GCNGC 5 cut(s) 487, 510, 522, 690, 1089
Sau3AI GATC 5 cut(s) 15, 59, 284, 302, 1056
Sau96I GGNCC 1 cut(s) 10
SchI GAGTC 2 cut(s) 135, 1201
ScrFI CCNGG 5 cut(s) 40, 113, 114, 1049, 1254
SduI GDGCHC 2 cut(s) 53, 1252
SfaNI GCATC 7 cut(s) 136, 229, 600, 676, 694, 726, 1186
SfcI CTRYAG 1 cut(s) 896
SfuI TTCGAA 1 cut(s) 1225
SinI GGWCC 1 cut(s) 10
SmaI CCCGGG 1 cut(s) 114
SmlI CTYRAG 2 cut(s) 379, 481
SmoI CTYRAG 2 cut(s) 379, 481
SpeI ACTAGT 1 cut(s) 614
Sse9I AATT 7 cut(s) 636, 666, 934, 942, 949, 1172, 1239
SsiI CCGC 6 cut(s) 8, 279, 438, 489, 524, 581
SspI AATATT 1 cut(s) 892
SspMI CTAG 4 cut(s) 131, 615, 914, 1193
StyD4I CCNGG 5 cut(s) 38, 111, 112, 1047, 1252
StyI CCWWGG 1 cut(s) 861
TaaI ACNGT 5 cut(s) 658, 679, 783, 1231, 1264
TaqI TCGA 4 cut(s) 18, 720, 1225, 1344
TaqII GACCGA 1 cut(s) 596
TasI AATT 7 cut(s) 636, 666, 934, 942, 949, 1172, 1239
TatI WGTACW 2 cut(s) 756, 1231
TfiI GAWTC 1 cut(s) 1138
Tru1I TTAA 3 cut(s) 1016, 1175, 1398
Tru9I TTAA 3 cut(s) 1016, 1175, 1398
TscAI CASTG 3 cut(s) 204, 300, 510
TseFI GTSAC 2 cut(s) 201, 1204
TseI GCWGC 5 cut(s) 486, 509, 521, 689, 1088
Tsp45I GTSAC 2 cut(s) 201, 1204
TspDTI ATGAA 2 cut(s) 284, 993
TspGWI ACGGA 1 cut(s) 813
TspMI CCCGGG 1 cut(s) 112
TspRI CASTG 3 cut(s) 204, 300, 510
Van91I CCANNNNNTGG 1 cut(s) 423
VpaK11BI GGWCC 1 cut(s) 10
VspI ATTAAT 1 cut(s) 1175
XagI CCTNNNNNAGG 2 cut(s) 17, 43
XceI RCATGY 2 cut(s) 88, 1014
XmaI CCCGGG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 4 cut(s) 131, 615, 914, 1193
Zsp2I ATGCAT 2 cut(s) 991, 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.