RLG00000015331

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000427
Physical Location & Seq
Forward (+)
64661669 .. 64665455
3787 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015331

Sequence Viewer

Length: 1521 bp
ATGGCAAAACCAAGCTTCCCCCACATCTTATCCTTATTGCTTCTCATCCTTCTCTTCACATTTTCATCTCTGCCAGGCACATTCGCAGCTCGTAGACTCTCTCTCAAAGAAGCTACCATAGACGATCTCCAGCTCGCTTTCCAGCACAACCAACTCACCTCGAGGCAACTTGTTCAATATTACCTTGCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCCAGAAGTCAACCCAGATGCGCTTCACCTTGCCGACAAGGCTGACAACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTACGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGCACACCACCGCTGGCTCTTTTGCGCTGTTTGGATCTGTCGTGCCTCGTGATGCTGGTGTGGTGGAGAAGTTGGGGAAAGCAGGGGCTATCATTTTGGGAAGGGCTAGCTTGAGCGAGTGGTCACATTTTACGTCTACTACAGTTCCCTCTAGTTGGAGCGCCAGAGGCGGTAATGGCTTGAACCCTTACACATTCACAGACCCGTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTGTCATTAGGTACCGAGACAAGTGTTGTGGGCCTCAAACCAACAGTCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCTAAGACAGGATATTGTTGGGCCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATTGAGACATCAAAGTACATTTCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGATGAACTTAGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGAAGGAGCTGTTTTGGTAGACAATTTGGAAATTGCTAATTTGATTGAAGTCTATTCTAGAAGCGATGAATATGCTACATTGTATACTGAGTGCAAAATAACCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCGGTGCGATCCTTGGCAGATGTTACAGCCTTCAACAACAAACACCAGAACCTAGAAAAGATCAAGGAGTATGGGCAAGACCGATTTTTAGCAGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCTGACATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTGATATTGGTGACAGAGAAGAAGCTAGATGCATTGGTGACTCCTTACGCAAGTTTGTCACGTGTACTTGCAATTGGGGGAGCCCCAGGTCTAACAGTTCCAGCTGGATACGAAAACAATGGGAAACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCCACTAAGATTAGGAAGCCTCCTTCATTCAAAGGCTTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

507

Amino Acids

54.71

Weight (kDa)

9.33

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 57 - 442 1.8e-57 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 160
Acc65I GGTACC 1 cut(s) 632
AccB1I GGYRCC 2 cut(s) 289, 632
AccI GTMKAC 4 cut(s) 94, 500, 984, 1049
AccII CGCG 1 cut(s) 858
AciI CCGC 5 cut(s) 375, 534, 573, 585, 620
AclWI GGATC 2 cut(s) 406, 1104
AcuI CTGAAG 1 cut(s) 1218
AcvI CACGTG 1 cut(s) 1334
AfaI GTAC 3 cut(s) 634, 821, 1338
AfiI CCNNNNNNNGG 2 cut(s) 519, 668
AflIII ACRYGT 1 cut(s) 1333
AgsI TTSAA 8 cut(s) 176, 547, 1013, 1084, 1135, 1243, 1504, 1513
AhlI ACTAGT 1 cut(s) 677
AjnI CCWGG 2 cut(s) 73, 1357
Alw26I GTCTC 2 cut(s) 632, 803
AlwI GGATC 2 cut(s) 406, 1104
AlwNI CAGNNNCTG 1 cut(s) 752
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 2 cut(s) 652, 725
ApeKI GCWGC 6 cut(s) 86, 582, 605, 617, 752, 1193
ArsI GACNNNNNNTTYG 2 cut(s) 651, 683
Asp718I GGTACC 1 cut(s) 632
AspLEI GCGC 5 cut(s) 247, 279, 391, 527, 858
AspS9I GGNCC 3 cut(s) 282, 652, 725
AsuC2I CCSGG 2 cut(s) 209, 1103
AsuHPI GGTGA 4 cut(s) 148, 242, 1294, 1321
AsuNHI GCTAGC 1 cut(s) 470
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 1 cut(s) 282
BanI GGYRCC 2 cut(s) 289, 632
BanII GRGCYC 1 cut(s) 1357
BauI CACGAG 1 cut(s) 411
BbrPI CACGTG 1 cut(s) 1334
BbvI GCAGC 6 cut(s) 98, 594, 617, 629, 739, 1205
BcgI CGANNNNNNTGC 2 cut(s) 1019, 1053
BciT130I CCWGG 2 cut(s) 75, 1359
BciVI GTATCC 1 cut(s) 1373
BcnI CCSGG 2 cut(s) 209, 1103
BcoDI GTCTC 2 cut(s) 632, 803
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 6 cut(s) 471, 516, 678, 1023, 1154, 1298
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 528
BfuI GTATCC 1 cut(s) 1373
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 7 cut(s) 87, 574, 583, 606, 618, 753, 1194
BlsI GCNGC 7 cut(s) 88, 575, 584, 607, 619, 754, 1195
Bme1390I CCNGG 4 cut(s) 75, 209, 1103, 1359
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 3 cut(s) 282, 652, 725
BmiI GGNNCC 3 cut(s) 291, 634, 1354
BmrFI CCNGG 4 cut(s) 75, 209, 1103, 1359
BmsI GCATC 7 cut(s) 232, 354, 406, 739, 757, 789, 1291
BmtI GCTAGC 1 cut(s) 474
BpmI CTGGAG 1 cut(s) 113
BpuEI CTTGAG 2 cut(s) 496, 562
BpuMI CCSGG 2 cut(s) 209, 1103
BsaAI YACGTR 1 cut(s) 1334
BsaJI CCNNGG 6 cut(s) 285, 354, 924, 1101, 1113, 1357
Bsc4I CCNNNNNNNGG 2 cut(s) 519, 668
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 3 cut(s) 345, 795, 919
BseBI CCWGG 2 cut(s) 75, 1359
BseDI CCNNGG 6 cut(s) 285, 354, 924, 1101, 1113, 1357
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 519, 668
BseMI GCAATG 3 cut(s) 345, 795, 919
BseMII CTCAG 3 cut(s) 865, 950, 1044
BseNI ACTGG 1 cut(s) 293
BseXI GCAGC 6 cut(s) 98, 594, 617, 629, 739, 1205
Bsh1236I CGCG 1 cut(s) 858
BshFI GGCC 2 cut(s) 654, 727
BshNI GGYRCC 2 cut(s) 289, 632
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 2 cut(s) 209, 1103
BslFI GGGAC 2 cut(s) 209, 680
BslI CCNNNNNNNGG 2 cut(s) 519, 668
BsmAI GTCTC 2 cut(s) 632, 803
BsmFI GGGAC 2 cut(s) 209, 680
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 2 cut(s) 654, 727
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 1357
Bsp143I GATC 4 cut(s) 124, 398, 1109, 1161
BspACI CCGC 5 cut(s) 375, 534, 573, 585, 620
BspANI GGCC 2 cut(s) 654, 727
BspCNI CTCAG 3 cut(s) 864, 951, 1045
BspFNI CGCG 1 cut(s) 858
BspLI GGNNCC 3 cut(s) 291, 634, 1354
BspOI GCTAGC 1 cut(s) 474
BspPI GGATC 2 cut(s) 406, 1104
BspT107I GGYRCC 2 cut(s) 289, 632
BsrDI GCAATG 3 cut(s) 345, 795, 919
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 6 cut(s) 285, 354, 924, 1101, 1113, 1357
BssMI GATC 4 cut(s) 124, 398, 1109, 1161
BssNAI GTATAC 1 cut(s) 1050
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 4 cut(s) 285, 354, 924, 1113
Bst1107I GTATAC 1 cut(s) 1050
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 2 cut(s) 75, 1359
Bst4CI ACNGT 5 cut(s) 508, 667, 742, 846, 1369
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 752
BstBAI YACGTR 1 cut(s) 1334
BstC8I GCNNGC 5 cut(s) 135, 379, 472, 778, 1473
BstDEI CTNAG 6 cut(s) 707, 851, 866, 959, 1053, 1479
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 1 cut(s) 858
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 5 cut(s) 247, 279, 391, 527, 858
BstKTI GATC 4 cut(s) 127, 401, 1112, 1164
BstMAI GTCTC 2 cut(s) 632, 803
BstMBI GATC 4 cut(s) 124, 398, 1109, 1161
BstMWI GCNNNNNNNGC 8 cut(s) 263, 531, 540, 579, 582, 614, 752, 1199
BstNI CCWGG 2 cut(s) 75, 1359
BstSCI CCNGG 4 cut(s) 73, 207, 1101, 1357
BstSFI CTRYAG 1 cut(s) 504
BstUI CGCG 1 cut(s) 858
BstV1I GCAGC 6 cut(s) 98, 594, 617, 629, 739, 1205
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BstZ17I GTATAC 1 cut(s) 1050
BsuI GTATCC 1 cut(s) 1373
BsuRI GGCC 2 cut(s) 654, 727
BtgZI GCGATG 2 cut(s) 873, 1044
BtsCI GGATG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 300, 606
Cac8I GCNNGC 5 cut(s) 135, 379, 472, 778, 1473
CaiI CAGNNNCTG 1 cut(s) 752
CfoI GCGC 5 cut(s) 247, 279, 391, 527, 858
Cfr13I GGNCC 3 cut(s) 282, 652, 725
Csp6I GTAC 3 cut(s) 633, 820, 1337
CspCI CAANNNNNGTGG 4 cut(s) 582, 617, 630, 665
CviAII CATG 1 cut(s) 317
CviQI GTAC 3 cut(s) 633, 820, 1337
DdeI CTNAG 6 cut(s) 707, 851, 866, 959, 1053, 1479
DpnI GATC 4 cut(s) 126, 400, 1111, 1163
DpnII GATC 4 cut(s) 124, 398, 1109, 1161
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 4 cut(s) 285, 354, 924, 1113
Eco24I GRGCYC 1 cut(s) 1357
Eco47I GGWCC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 1218
Eco72I CACGTG 1 cut(s) 1334
Eco88I CYCGRG 1 cut(s) 160
EcoRII CCWGG 2 cut(s) 73, 1357
EcoT14I CCWWGG 4 cut(s) 285, 354, 924, 1113
EcoT22I ATGCAT 2 cut(s) 1078, 1306
EcoT38I GRGCYC 1 cut(s) 1357
ErhI CCWWGG 4 cut(s) 285, 354, 924, 1113
FaeI CATG 1 cut(s) 320
FaqI GGGAC 2 cut(s) 209, 680
FatI CATG 1 cut(s) 316
FauNDI CATATG 1 cut(s) 1459
FblI GTMKAC 4 cut(s) 94, 500, 984, 1049
Fnu4HI GCNGC 7 cut(s) 87, 574, 583, 606, 618, 753, 1194
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 1357
Fsp4HI GCNGC 7 cut(s) 87, 574, 583, 606, 618, 753, 1194
FspBI CTAG 6 cut(s) 471, 516, 678, 1023, 1154, 1298
GlaI GCGC 5 cut(s) 246, 278, 390, 526, 857
GluI GCNGC 7 cut(s) 87, 574, 583, 606, 618, 753, 1194
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 2 cut(s) 654, 727
HapII CCGG 2 cut(s) 209, 1103
HhaI GCGC 5 cut(s) 247, 279, 391, 527, 858
Hin1II CATG 1 cut(s) 320
Hin6I GCGC 5 cut(s) 245, 277, 389, 525, 856
HinP1I GCGC 5 cut(s) 245, 277, 389, 525, 856
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 4 cut(s) 96, 222, 1243, 1312
HpaII CCGG 2 cut(s) 209, 1103
HphI GGTGA 4 cut(s) 148, 242, 1294, 1321
Hpy166II GTNNAC 8 cut(s) 95, 235, 501, 985, 1050, 1257, 1337, 1421
Hpy188I TCNGA 3 cut(s) 197, 960, 1435
Hpy188III TCNNGA 4 cut(s) 413, 764, 1023, 1450
Hpy8I GTNNAC 8 cut(s) 95, 235, 501, 985, 1050, 1257, 1337, 1421
HpyAV CCTTC 7 cut(s) 59, 459, 907, 962, 1078, 1141, 1506
HpyCH4III ACNGT 5 cut(s) 508, 667, 742, 846, 1369
HpyCH4IV ACGT 3 cut(s) 311, 497, 1333
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 531, 540, 579, 582, 614, 752, 1199
HpyF3I CTNAG 6 cut(s) 707, 851, 866, 959, 1053, 1479
HpySE526I ACGT 3 cut(s) 311, 497, 1333
Hsp92II CATG 1 cut(s) 320
HspAI GCGC 5 cut(s) 245, 277, 389, 525, 856
KpnI GGTACC 1 cut(s) 636
Kzo9I GATC 4 cut(s) 124, 398, 1109, 1161
LmnI GCTCC 3 cut(s) 522, 971, 1352
Lsp1109I GCAGC 6 cut(s) 98, 594, 617, 629, 739, 1205
LweI GCATC 7 cut(s) 232, 354, 406, 739, 757, 789, 1291
MaeI CTAG 6 cut(s) 471, 516, 678, 1023, 1154, 1298
MaeII ACGT 3 cut(s) 311, 497, 1333
MaeIII GTNAC 6 cut(s) 297, 486, 1123, 1282, 1309, 1329
MalI GATC 4 cut(s) 126, 400, 1111, 1163
MboI GATC 4 cut(s) 124, 398, 1109, 1161
MboII GAAGA 2 cut(s) 46, 1303
MfeI CAATTG 1 cut(s) 1344
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 1357
MluCI AATT 6 cut(s) 699, 729, 988, 996, 1003, 1344
MlyI GAGTC 3 cut(s) 90, 231, 1306
MmeI TCCRAC 1 cut(s) 500
MnlI CCTC 9 cut(s) 156, 169, 420, 523, 524, 665, 863, 954, 1503
Mph1103I ATGCAT 2 cut(s) 1078, 1306
MseI TTAA 1 cut(s) 1070
MspA1I CMGCKG 6 cut(s) 377, 585, 620, 1196, 1376, 1403
MspI CCGG 2 cut(s) 209, 1103
MspR9I CCNGG 4 cut(s) 75, 209, 1103, 1359
MunI CAATTG 1 cut(s) 1344
Mva1269I GAATGC 1 cut(s) 321
MvaI CCWGG 2 cut(s) 75, 1359
MvnI CGCG 1 cut(s) 858
MwoI GCNNNNNNNGC 8 cut(s) 263, 531, 540, 579, 582, 614, 752, 1199
NciI CCSGG 2 cut(s) 209, 1103
NdeI CATATG 1 cut(s) 1459
NdeII GATC 4 cut(s) 124, 398, 1109, 1161
NheI GCTAGC 1 cut(s) 470
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 3 cut(s) 291, 634, 1354
NmuCI GTSAC 5 cut(s) 297, 486, 1282, 1309, 1329
NsiI ATGCAT 2 cut(s) 1078, 1306
PaeR7I CTCGAG 1 cut(s) 160
PctI GAATGC 1 cut(s) 321
PfeI GAWTC 1 cut(s) 1243
PkrI GCNGC 7 cut(s) 88, 575, 584, 607, 619, 754, 1195
PleI GAGTC 3 cut(s) 90, 230, 1306
PmaCI CACGTG 1 cut(s) 1334
PmlI CACGTG 1 cut(s) 1334
PpsI GAGTC 3 cut(s) 90, 230, 1306
Ppu21I YACGTR 1 cut(s) 1334
Psp6I CCWGG 2 cut(s) 73, 1357
PspCI CACGTG 1 cut(s) 1334
PspGI CCWGG 2 cut(s) 73, 1357
PspN4I GGNNCC 3 cut(s) 291, 634, 1354
PspPI GGNCC 3 cut(s) 282, 652, 725
PspXI VCTCGAGB 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 1 cut(s) 398
PvuII CAGCTG 3 cut(s) 1196, 1376, 1403
RsaI GTAC 3 cut(s) 634, 821, 1338
RsaNI GTAC 3 cut(s) 633, 820, 1337
SaqAI TTAA 1 cut(s) 1070
SatI GCNGC 7 cut(s) 87, 574, 583, 606, 618, 753, 1194
Sau3AI GATC 4 cut(s) 124, 398, 1109, 1161
Sau96I GGNCC 3 cut(s) 282, 652, 725
SchI GAGTC 3 cut(s) 90, 231, 1306
ScrFI CCNGG 4 cut(s) 75, 209, 1103, 1359
SduI GDGCHC 1 cut(s) 1357
SfaNI GCATC 7 cut(s) 232, 354, 406, 739, 757, 789, 1291
SfcI CTRYAG 1 cut(s) 504
Sfr274I CTCGAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 282
SlaI CTCGAG 1 cut(s) 160
SmlI CTYRAG 3 cut(s) 160, 475, 577
SmoI CTYRAG 3 cut(s) 160, 475, 577
SpeI ACTAGT 1 cut(s) 677
Sse9I AATT 6 cut(s) 699, 729, 988, 996, 1003, 1344
SsiI CCGC 5 cut(s) 375, 534, 573, 585, 620
SspI AATATT 1 cut(s) 179
SspMI CTAG 6 cut(s) 471, 516, 678, 1023, 1154, 1298
StyD4I CCNGG 4 cut(s) 73, 207, 1101, 1357
StyI CCWWGG 4 cut(s) 285, 354, 924, 1113
TaaI ACNGT 5 cut(s) 508, 667, 742, 846, 1369
TaiI ACGT 3 cut(s) 314, 500, 1336
TaqI TCGA 4 cut(s) 161, 682, 783, 1449
TaqII GACCGA 2 cut(s) 659, 1197
TasI AATT 6 cut(s) 699, 729, 988, 996, 1003, 1344
TatI WGTACW 2 cut(s) 819, 1336
TauI GCSGC 1 cut(s) 576
TfiI GAWTC 1 cut(s) 1243
Tru1I TTAA 1 cut(s) 1070
Tru9I TTAA 1 cut(s) 1070
TscAI CASTG 2 cut(s) 300, 606
TseFI GTSAC 5 cut(s) 297, 486, 1282, 1309, 1329
TseI GCWGC 6 cut(s) 86, 582, 605, 617, 752, 1193
Tsp45I GTSAC 5 cut(s) 297, 486, 1282, 1309, 1329
TspDTI ATGAA 4 cut(s) 54, 876, 1047, 1488
TspRI CASTG 2 cut(s) 300, 606
VpaK11BI GGWCC 1 cut(s) 282
XbaI TCTAGA 1 cut(s) 1022
XhoI CTCGAG 1 cut(s) 160
XmiI GTMKAC 4 cut(s) 94, 500, 984, 1049
XspI CTAG 6 cut(s) 471, 516, 678, 1023, 1154, 1298
Zsp2I ATGCAT 2 cut(s) 1078, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.