Rw6G000010

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000427
Physical Location & Seq
Forward (+)
62699 .. 64392
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000010.1

Sequence Viewer

Length: 873 bp
ATGACCTTAGAAAAAATAATAGGTAGAGTCTGGTGGCGTCTGCAGAATAAACAACTGGAGTCCTTGAAAGAACATAAAATAACTTCAGAAATAGATATGGCTACCATTTCGTGTTCTCTCTTCTGGGTTCTCCTTCTAATTCTTCAAAGCACACTATCAAACGGGTCTGAATCCATTACACCCAGAAGCCTAATGGAAGAAGCCACAGTTGATGATCTCCAACTCGCTTTCAAGCAAAAGCTTCTGACTTCAAGGCAAGTCGTTCAGTTCTACCTGAACCAAATCCACAAGCTCAATCAAGACCTCAGAGGAGTCTTAGAGATAAACCCTGATGCTTTATCGCAAGCTGACATAGCAGACAAAGAGCGCAGTGAATATGAATCCAGTGCCCAAACACTGCCTAAGCTTCATGGGATTCCTGCTCTGCTCAAGGATAATATAGCAACCAAGGACAAGCCTAACACCACTGCTGGGTCTTATGCTCTTTTGGGTTCCGTAGTGCCTAGAGATGCTGGGGTTGTCTCAAAGTTGAGGAGCTCTGGGGCTATCATTTTGGGGAAGGCTACTTTGAGTGAATGGGCCTATATCAGAACATCTAAAGCACCCTATGGTTGGAGTGCCAGAGGTGGTCAAGGAGTGAATCCTTATAATTCATCACTGGAAGTTTGTGGATCAAGTAGTGGATCCGCAATATCAGTATCTGCAAATATGGTATCAGTGTCTCTAGGAACAGAGACCTATAGCTCCATCTTATGTCCAGCGAGTTTTAACTCGGTAGTGGGCTTCAAACCAACGGTTGGTCTCACTAGTCGAGCAGGAGTCATCCCAGTCAGTCCAAGACAGGACACAATTGGGCCAATTGTAGGACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

290

Amino Acids

31.27

Weight (kDa)

8.68

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 87 - 287 2.6e-56 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AccB7I CCANNNNNTGG 1 cut(s) 797
AciI CCGC 1 cut(s) 687
AclWI GGATC 3 cut(s) 678, 679, 691
AcuI CTGAAG 1 cut(s) 69
AcyI GRCGYC 1 cut(s) 37
AfiI CCNNNNNNNGG 4 cut(s) 471, 612, 797, 863
AgsI TTSAA 5 cut(s) 67, 146, 232, 252, 787
AhlI ACTAGT 1 cut(s) 806
AluBI AGCT 6 cut(s) 241, 292, 347, 406, 537, 744
AluI AGCT 6 cut(s) 241, 292, 347, 406, 537, 744
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 4 cut(s) 526, 726, 728, 806
AlwI GGATC 3 cut(s) 678, 679, 691
AlwNI CAGNNNCTG 1 cut(s) 701
AoxI GGCC 2 cut(s) 579, 854
AspLEI GCGC 1 cut(s) 369
AspS9I GGNCC 2 cut(s) 579, 854
BaeGI GKGCMC 1 cut(s) 391
BamHI GGATCC 1 cut(s) 683
BanII GRGCYC 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 539
BccI CCATC 1 cut(s) 755
BcoDI GTCTC 4 cut(s) 526, 726, 728, 806
BcuI ACTAGT 1 cut(s) 806
BfaI CTAG 3 cut(s) 504, 725, 807
BfmI CTRYAG 3 cut(s) 41, 739, 869
BmgT120I GGNCC 2 cut(s) 579, 854
BmiI GGNNCC 2 cut(s) 493, 685
BmrI ACTGGG 1 cut(s) 821
BmsI GCATC 2 cut(s) 322, 499
BmuI ACTGGG 1 cut(s) 821
BpmI CTGGAG 1 cut(s) 77
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 1 cut(s) 413
BsaHI GRCGYC 1 cut(s) 37
BsaI GGTCTC 2 cut(s) 728, 806
BsaJI CCNNGG 1 cut(s) 447
Bsc4I CCNNNNNNNGG 4 cut(s) 471, 612, 797, 863
Bse1I ACTGG 4 cut(s) 60, 384, 663, 827
BseDI CCNNGG 1 cut(s) 447
BseGI GGATG 1 cut(s) 822
BseLI CCNNNNNNNGG 4 cut(s) 471, 612, 797, 863
BseMII CTCAG 1 cut(s) 319
BseNI ACTGG 4 cut(s) 60, 384, 663, 827
BseRI GAGGAG 2 cut(s) 324, 547
BseSI GKGCMC 1 cut(s) 391
BseYI CCCAGC 2 cut(s) 470, 512
BshFI GGCC 2 cut(s) 581, 856
BsiHKAI GWGCWC 1 cut(s) 539
BslI CCNNNNNNNGG 4 cut(s) 471, 612, 797, 863
BsmAI GTCTC 4 cut(s) 526, 726, 728, 806
BsnI GGCC 2 cut(s) 581, 856
Bso31I GGTCTC 2 cut(s) 728, 806
Bsp1286I GDGCHC 2 cut(s) 391, 539
Bsp143I GATC 3 cut(s) 214, 671, 683
BspACI CCGC 1 cut(s) 687
BspANI GGCC 2 cut(s) 581, 856
BspCNI CTCAG 1 cut(s) 318
BspLI GGNNCC 2 cut(s) 493, 685
BspMAI CTGCAG 1 cut(s) 45
BspPI GGATC 3 cut(s) 678, 679, 691
BspTNI GGTCTC 2 cut(s) 728, 806
BsrI ACTGG 4 cut(s) 60, 384, 663, 827
BssECI CCNNGG 1 cut(s) 447
BssMI GATC 3 cut(s) 214, 671, 683
BssNI GRCGYC 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 447
Bst4CI ACNGT 3 cut(s) 208, 796, 870
Bst6I CTCTTC 1 cut(s) 125
BstACI GRCGYC 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 4 cut(s) 7, 305, 316, 402
BstF5I GGATG 1 cut(s) 822
BstHHI GCGC 1 cut(s) 369
BstKTI GATC 3 cut(s) 217, 674, 686
BstMAI GTCTC 4 cut(s) 526, 726, 728, 806
BstMBI GATC 3 cut(s) 214, 671, 683
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstSFI CTRYAG 3 cut(s) 41, 739, 869
BstSLI GKGCMC 1 cut(s) 391
BstX2I RGATCY 1 cut(s) 683
BstYI RGATCY 1 cut(s) 683
BsuRI GGCC 2 cut(s) 581, 856
BtsCI GGATG 1 cut(s) 822
BtsI GCAGTG 3 cut(s) 376, 395, 465
BtsIMutI CAGTG 6 cut(s) 376, 391, 395, 465, 656, 723
Cac8I GCNNGC 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 701
CfoI GCGC 1 cut(s) 369
Cfr13I GGNCC 2 cut(s) 579, 854
CseI GACGC 1 cut(s) 26
CviAII CATG 1 cut(s) 410
DdeI CTNAG 4 cut(s) 7, 305, 316, 402
DpnI GATC 3 cut(s) 216, 673, 685
DpnII GATC 3 cut(s) 214, 671, 683
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 539
Eco31I GGTCTC 2 cut(s) 728, 806
Eco53kI GAGCTC 1 cut(s) 537
Eco57I CTGAAG 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 537
EcoT14I CCWWGG 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 539
ErhI CCWWGG 1 cut(s) 447
FaeI CATG 1 cut(s) 413
FatI CATG 1 cut(s) 409
FokI GGATG 1 cut(s) 809
FriOI GRGCYC 1 cut(s) 539
FspBI CTAG 3 cut(s) 504, 725, 807
GlaI GCGC 1 cut(s) 368
GsaI CCCAGC 2 cut(s) 474, 516
GsuI CTGGAG 1 cut(s) 77
HaeIII GGCC 2 cut(s) 581, 856
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 369
Hin1I GRCGYC 1 cut(s) 37
Hin1II CATG 1 cut(s) 413
Hin6I GCGC 1 cut(s) 367
HinP1I GCGC 1 cut(s) 367
HindIII AAGCTT 2 cut(s) 239, 404
HinfI GANTC 8 cut(s) 27, 59, 170, 312, 380, 415, 640, 819
Hpy188I TCNGA 5 cut(s) 88, 169, 246, 308, 590
Hpy188III TCNNGA 1 cut(s) 299
HpyAV CCTTC 2 cut(s) 143, 553
HpyCH4III ACNGT 3 cut(s) 208, 796, 870
HpyCH4V TGCA 2 cut(s) 43, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 4 cut(s) 7, 305, 316, 402
Hsp92I GRCGYC 1 cut(s) 37
Hsp92II CATG 1 cut(s) 413
HspAI GCGC 1 cut(s) 367
Kzo9I GATC 3 cut(s) 214, 671, 683
LmnI GCTCC 2 cut(s) 534, 749
LweI GCATC 2 cut(s) 322, 499
MaeI CTAG 3 cut(s) 504, 725, 807
MalI GATC 3 cut(s) 216, 673, 685
MboI GATC 3 cut(s) 214, 671, 683
MboII GAAGA 3 cut(s) 112, 134, 209
MfeI CAATTG 2 cut(s) 849, 858
MflI RGATCY 1 cut(s) 683
MhlI GDGCHC 2 cut(s) 391, 539
MluCI AATT 4 cut(s) 138, 649, 849, 858
MlyI GAGTC 4 cut(s) 36, 68, 321, 828
MmeI TCCRAC 2 cut(s) 244, 593
MnlI CCTC 4 cut(s) 302, 314, 525, 617
MseI TTAA 1 cut(s) 768
MunI CAATTG 2 cut(s) 849, 858
MwoI GCNNNNNNNGC 1 cut(s) 353
NdeII GATC 3 cut(s) 214, 671, 683
NlaIII CATG 1 cut(s) 413
NlaIV GGNNCC 2 cut(s) 493, 685
PfeI GAWTC 4 cut(s) 170, 380, 415, 640
PflMI CCANNNNNTGG 1 cut(s) 797
PleI GAGTC 4 cut(s) 35, 67, 320, 827
PpsI GAGTC 4 cut(s) 35, 67, 320, 827
PsiI TTATAA 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 539
PspFI CCCAGC 2 cut(s) 470, 512
PspN4I GGNNCC 2 cut(s) 493, 685
PspPI GGNCC 2 cut(s) 579, 854
PstI CTGCAG 1 cut(s) 45
PstNI CAGNNNCTG 1 cut(s) 701
PsuI RGATCY 1 cut(s) 683
SacI GAGCTC 1 cut(s) 539
SaqAI TTAA 1 cut(s) 768
Sau3AI GATC 3 cut(s) 214, 671, 683
Sau96I GGNCC 2 cut(s) 579, 854
SchI GAGTC 4 cut(s) 36, 68, 321, 828
SduI GDGCHC 2 cut(s) 391, 539
SfaNI GCATC 2 cut(s) 322, 499
SfcI CTRYAG 3 cut(s) 41, 739, 869
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SpeI ACTAGT 1 cut(s) 806
Sse9I AATT 4 cut(s) 138, 649, 849, 858
SsiI CCGC 1 cut(s) 687
SspMI CTAG 3 cut(s) 504, 725, 807
SstI GAGCTC 1 cut(s) 539
StyI CCWWGG 1 cut(s) 447
TaaI ACNGT 3 cut(s) 208, 796, 870
TaqI TCGA 1 cut(s) 811
TasI AATT 4 cut(s) 138, 649, 849, 858
TfiI GAWTC 4 cut(s) 170, 380, 415, 640
Tru1I TTAA 1 cut(s) 768
Tru9I TTAA 1 cut(s) 768
TscAI CASTG 6 cut(s) 376, 391, 402, 472, 663, 723
TspDTI ATGAA 3 cut(s) 393, 398, 642
TspGWI ACGGA 1 cut(s) 484
TspRI CASTG 6 cut(s) 376, 391, 402, 472, 663, 723
Van91I CCANNNNNTGG 1 cut(s) 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 190
XspI CTAG 3 cut(s) 504, 725, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.