MD10G1120200.v1.1

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000427
Physical Location & Seq
Forward (+)
19688728 .. 19691338
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1120200.v1.1.491

Sequence Viewer

Length: 1554 bp
ATGGAGAAACCAAACCTCCATCACTTCTCATCCTTACTTCTGGTTTTTCTGTTTACATTGTCGCTTGGGACTCGGACAACGGCGGGCCGGAAATTCTCGATCAGGGAAGCCACCATTGACGATCTCCAACTCGCTTTCAAGCACAACCTACTCACCTCAAGGCAACTTGTCCAGTTCTACCTTGGAGAAATCAAAAGACTCAACCCTTTCCTTAGGGCGGTCATAGAAGTGAACCCGGACGCGCTTTACTTGGCTGACAAGGCGGATTATGAACGCAAGACTAAGGCGCCGTCCGCGTCACACTCTAAGTTACATGGGATTCCTATTCTGGTCAAGGACAACATTGCTACCAAGGACAAGTTGAACACCACAGCTGGCTCGTATGCTCTGCTTGGCTCGGTTGTTCCTCGTGATGCGGGTGTTGTCACCAAGCTGAGGAATGCCGGGGCTATTATACTGGGAAAGGCCAGCCTGAGTGAGTGGTCCCATTGGAGGTCCAACGGTGCACCTTCCGGTTGGAGTGCCAGAGGTGGCCAAGGGGTGAACCCTTACAATTCTCTTGCAACACCTTGCGGCTCAAGTAGCGGTTCAGCAATATCAGCGGCAGCAAATCTGGCAACAGTGACACTCGGGACTGAAACAGATGGATCAATTTTATGTCCATCCAGTTATAACTCAGTCGTGGGCATCAAACCAACAAACGGTCTTACTAGTCGAGCAGGGGTAGTCCCAATCTCCCTAAGACAGGACACAGTTGGGCCAATTTGTAGGACAGTATCAGATGCTGCTTATGTTCTTGATGTCATTGCAGGCATCGACCAGAACGATGTTGCAACGACTAAGGCATCGAGGTACATTCCCAAGGGTGGCTACGCACAGTTCCTCAGGCGTGATGGGCTTAGAGGAAAGCGAATAGGGATAGTGAGAGCCTTATTTAACATCTCCGGCCAAACTTCGTCGACTCCAATTTATGAAAAACACTTCAACACGCTGAGGAAAGGTGGTGCTGTTTTGGTGGACAATTTGAAAATACCACATTTTGATGAAATCTATTCCGATGGAAGTAGTGAAACTACTGCACTGTCAGCTGAGTTCAAAATATATATAAACACATACCTTAAGGAGTTGGTGGCTTCGCCAGTCCGATCCTTGGCGGATTTGATAGCCTTTAACAATAAAAACGCTAAATTGGAGAAGGTGAAGGAGTATGGCCAAGGCCTATTTTTAGAAGCTGAAGCAACAAATGGAATAGGAAGTGCAGAGAAGGCAGCATTGTTAAATTTGGCAAGATTTGCGAAGGATGACTACGTGAATTTAGTGACGAAGAACAAGCTAGATGCCGTGGTGGCTCCTCTTGCACAAGTGTCGCCGTTGCTAGCGATTAGCGGCTCCCCGGGTGTGGTTGTCCCAGCAGGATATGATGATGGGGTACCAGTTGGCCTTTCCTTTGGGGGGCTGAGGGGTTGGGAGCCAAAGCTAATTGAAATGGCATATGGCTTTGAGCAAGCAACTAAGATTAGGAAGCCTCCTTCACTTACAAAGTTGAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

518

Amino Acids

55.41

Weight (kDa)

9.51

Isoelectric Point (pI)

28.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 56 - 457 6.1e-71 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 672
Acc65I GGTACC 1 cut(s) 1429
AccB1I GGYRCC 2 cut(s) 286, 1429
AccI GTMKAC 1 cut(s) 959
AccII CGCG 2 cut(s) 242, 296
AclWI GGATC 2 cut(s) 655, 1140
AcoI YGGCCR 3 cut(s) 532, 946, 1210
AcsI RAATTY 3 cut(s) 92, 1279, 1312
AcuI CTGAAG 1 cut(s) 1254
AcyI GRCGYC 1 cut(s) 287
AfaI GTAC 2 cut(s) 854, 1431
AfiI CCNNNNNNNGG 9 cut(s) 217, 435, 492, 701, 745, 866, 1150, 1399, 1452
AflII CTTAAG 1 cut(s) 1118
AgsI TTSAA 7 cut(s) 139, 364, 985, 1027, 1096, 1484, 1546
AhlI ACTAGT 1 cut(s) 710
AluBI AGCT 6 cut(s) 374, 433, 1088, 1232, 1333, 1477
AluI AGCT 6 cut(s) 374, 433, 1088, 1232, 1333, 1477
Alw21I GWGCWC 1 cut(s) 508
Alw44I GTGCAC 1 cut(s) 504
AlwI GGATC 2 cut(s) 655, 1140
AlwNI CAGNNNCTG 1 cut(s) 785
Ama87I CYCGRG 2 cut(s) 629, 1393
AoxI GGCC 8 cut(s) 85, 465, 532, 758, 946, 1210, 1216, 1438
ApaLI GTGCAC 1 cut(s) 504
ApeKI GCWGC 3 cut(s) 605, 785, 1268
ApoI RAATTY 3 cut(s) 92, 1279, 1312
Asp718I GGTACC 1 cut(s) 1429
AspLEI GCGC 2 cut(s) 244, 289
AspS9I GGNCC 4 cut(s) 85, 483, 495, 758
AsuC2I CCSGG 4 cut(s) 236, 445, 1394, 1395
AsuHPI GGTGA 4 cut(s) 145, 418, 553, 1210
AsuNHI GCTAGC 1 cut(s) 1375
AvaI CYCGRG 2 cut(s) 629, 1393
AvaII GGWCC 2 cut(s) 483, 495
AxyI CCTNAGG 2 cut(s) 212, 884
BaeGI GKGCMC 1 cut(s) 508
BalI TGGCCA 2 cut(s) 534, 1212
BanI GGYRCC 2 cut(s) 286, 1429
BarI GAAGNNNNNNTAC 2 cut(s) 1289, 1321
BauI CACGAG 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 508
BbvCI CCTCAGC 3 cut(s) 434, 992, 1457
BbvI GCAGC 3 cut(s) 617, 772, 1280
BccI CCATC 6 cut(s) 27, 638, 670, 887, 1052, 1418
BceAI ACGGC 4 cut(s) 96, 274, 1325, 1354
BcgI CGANNNNNNTGC 2 cut(s) 1347, 1381
BcnI CCSGG 4 cut(s) 236, 445, 1394, 1395
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 3 cut(s) 711, 1334, 1376
BfoI RGCGCY 1 cut(s) 290
BfrI CTTAAG 1 cut(s) 1118
BglI GCCNNNNNGGC 1 cut(s) 1346
BisI GCNGC 6 cut(s) 574, 603, 606, 786, 1269, 1387
BlsI GCNGC 6 cut(s) 575, 604, 607, 787, 1270, 1388
Bme1390I CCNGG 4 cut(s) 236, 445, 1394, 1395
Bme18I GGWCC 2 cut(s) 483, 495
BmeT110I CYCGRG 2 cut(s) 629, 1393
BmgT120I GGNCC 4 cut(s) 85, 483, 495, 758
BmiI GGNNCC 6 cut(s) 288, 485, 1350, 1390, 1431, 1470
BmrFI CCNGG 4 cut(s) 236, 445, 1394, 1395
BmrI ACTGGG 1 cut(s) 467
BmsI GCATC 6 cut(s) 403, 696, 772, 822, 854, 1327
BmtI GCTAGC 1 cut(s) 1379
BmuI ACTGGG 1 cut(s) 467
Bpu10I CCTNAGC 3 cut(s) 434, 992, 1457
BpuEI CTTGAG 2 cut(s) 142, 562
BpuMI CCSGG 4 cut(s) 236, 445, 1394, 1395
BsaAI YACGTR 1 cut(s) 1309
BsaHI GRCGYC 1 cut(s) 287
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 9 cut(s) 217, 435, 492, 701, 745, 866, 1150, 1399, 1452
Bse1I ACTGG 5 cut(s) 172, 462, 666, 1139, 1433
Bse21I CCTNAGG 2 cut(s) 212, 884
Bse3DI GCAATG 2 cut(s) 342, 804
BseGI GGATG 3 cut(s) 29, 662, 1306
BseLI CCNNNNNNNGG 9 cut(s) 217, 435, 492, 701, 745, 866, 1150, 1399, 1452
BseMI GCAATG 2 cut(s) 342, 804
BseMII CTCAG 7 cut(s) 425, 464, 690, 898, 983, 1080, 1448
BseNI ACTGG 5 cut(s) 172, 462, 666, 1139, 1433
BseRI GAGGAG 1 cut(s) 1341
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 3 cut(s) 617, 772, 1280
BseYI CCCAGC 1 cut(s) 1408
BsgI GTGCAG 2 cut(s) 1062, 1278
Bsh1236I CGCG 2 cut(s) 242, 296
BshFI GGCC 8 cut(s) 87, 467, 534, 760, 948, 1212, 1218, 1440
BshNI GGYRCC 2 cut(s) 286, 1429
BsiHKAI GWGCWC 1 cut(s) 508
BsiHKCI CYCGRG 2 cut(s) 629, 1393
BsiSI CCGG 6 cut(s) 88, 236, 444, 513, 945, 1394
BslFI GGGAC 5 cut(s) 82, 469, 646, 713, 1391
BslI CCNNNNNNNGG 9 cut(s) 217, 435, 492, 701, 745, 866, 1150, 1399, 1452
BsmFI GGGAC 5 cut(s) 82, 469, 646, 713, 1391
BsmI GAATGC 1 cut(s) 445
BsnI GGCC 8 cut(s) 87, 467, 534, 760, 948, 1212, 1218, 1440
BsoBI CYCGRG 2 cut(s) 629, 1393
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 4 cut(s) 99, 121, 647, 1145
BspANI GGCC 8 cut(s) 87, 467, 534, 760, 948, 1212, 1218, 1440
BspCNI CTCAG 7 cut(s) 426, 465, 689, 897, 984, 1081, 1449
BspFNI CGCG 2 cut(s) 242, 296
BspLI GGNNCC 6 cut(s) 288, 485, 1350, 1390, 1431, 1470
BspOI GCTAGC 1 cut(s) 1379
BspPI GGATC 2 cut(s) 655, 1140
BspT107I GGYRCC 2 cut(s) 286, 1429
BspTI CTTAAG 1 cut(s) 1118
BsrDI GCAATG 2 cut(s) 342, 804
BsrI ACTGG 5 cut(s) 172, 462, 666, 1139, 1433
BssMI GATC 4 cut(s) 99, 121, 647, 1145
BssNI GRCGYC 1 cut(s) 287
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 6 cut(s) 181, 351, 535, 861, 1149, 1213
Bst2BI CACGAG 1 cut(s) 408
Bst4CI ACNGT 7 cut(s) 503, 622, 704, 754, 775, 879, 1083
BstACI GRCGYC 1 cut(s) 287
BstAFI CTTAAG 1 cut(s) 1118
BstAPI GCANNNNNTGC 1 cut(s) 1292
BstBAI YACGTR 1 cut(s) 1309
BstC8I GCNNGC 6 cut(s) 85, 376, 469, 811, 1377, 1506
BstDSI CCRYGG 1 cut(s) 1341
BstENI CCTNNNNNAGG 1 cut(s) 743
BstF5I GGATG 3 cut(s) 29, 662, 1306
BstFNI CGCG 2 cut(s) 242, 296
BstH2I RGCGCY 1 cut(s) 290
BstHHI GCGC 2 cut(s) 244, 289
BstKTI GATC 4 cut(s) 102, 124, 650, 1148
BstMBI GATC 4 cut(s) 99, 121, 647, 1145
BstSCI CCNGG 4 cut(s) 234, 443, 1392, 1393
BstSLI GKGCMC 1 cut(s) 508
BstUI CGCG 2 cut(s) 242, 296
BstV1I GCAGC 3 cut(s) 617, 772, 1280
Bsu36I CCTNAGG 2 cut(s) 212, 884
BsuRI GGCC 8 cut(s) 87, 467, 534, 760, 948, 1212, 1218, 1440
BtgI CCRYGG 1 cut(s) 1341
BtsCI GGATG 3 cut(s) 29, 662, 1306
BtsIMutI CAGTG 2 cut(s) 627, 1079
Cac8I GCNNGC 6 cut(s) 85, 376, 469, 811, 1377, 1506
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 2 cut(s) 244, 289
Cfr13I GGNCC 4 cut(s) 85, 483, 495, 758
Cfr9I CCCGGG 1 cut(s) 1393
CseI GACGC 2 cut(s) 248, 285
Csp6I GTAC 2 cut(s) 853, 1430
CviAII CATG 1 cut(s) 314
CviQI GTAC 2 cut(s) 853, 1430
DinI GGCGCC 1 cut(s) 288
DpnI GATC 4 cut(s) 101, 123, 649, 1147
DpnII GATC 4 cut(s) 99, 121, 647, 1145
EaeI YGGCCR 3 cut(s) 532, 946, 1210
EciI GGCGGA 2 cut(s) 278, 1169
Eco130I CCWWGG 6 cut(s) 181, 351, 535, 861, 1149, 1213
Eco147I AGGCCT 1 cut(s) 1218
Eco47I GGWCC 2 cut(s) 483, 495
Eco57I CTGAAG 1 cut(s) 1254
Eco81I CCTNAGG 2 cut(s) 212, 884
Eco88I CYCGRG 2 cut(s) 629, 1393
EcoNI CCTNNNNNAGG 1 cut(s) 743
EcoT14I CCWWGG 6 cut(s) 181, 351, 535, 861, 1149, 1213
EgeI GGCGCC 1 cut(s) 288
EheI GGCGCC 1 cut(s) 288
ErhI CCWWGG 6 cut(s) 181, 351, 535, 861, 1149, 1213
FaeI CATG 1 cut(s) 317
FaqI GGGAC 5 cut(s) 82, 469, 646, 713, 1391
FatI CATG 1 cut(s) 313
FauI CCCGC 2 cut(s) 76, 409
FauNDI CATATG 1 cut(s) 1492
FblI GTMKAC 1 cut(s) 959
Fnu4HI GCNGC 6 cut(s) 574, 603, 606, 786, 1269, 1387
FokI GGATG 3 cut(s) 16, 649, 1313
Fsp4HI GCNGC 6 cut(s) 574, 603, 606, 786, 1269, 1387
FspBI CTAG 3 cut(s) 711, 1334, 1376
GlaI GCGC 2 cut(s) 243, 288
GluI GCNGC 6 cut(s) 574, 603, 606, 786, 1269, 1387
GsaI CCCAGC 1 cut(s) 1412
HaeII RGCGCY 1 cut(s) 290
HaeIII GGCC 8 cut(s) 87, 467, 534, 760, 948, 1212, 1218, 1440
HapII CCGG 6 cut(s) 88, 236, 444, 513, 945, 1394
HgaI GACGC 2 cut(s) 248, 285
HhaI GCGC 2 cut(s) 244, 289
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 1 cut(s) 317
Hin6I GCGC 2 cut(s) 242, 287
HinP1I GCGC 2 cut(s) 242, 287
HincII GTYRAC 1 cut(s) 960
HindII GTYRAC 1 cut(s) 960
HinfI GANTC 4 cut(s) 70, 198, 319, 961
HpaII CCGG 6 cut(s) 88, 236, 444, 513, 945, 1394
HphI GGTGA 4 cut(s) 145, 418, 553, 1210
Hpy166II GTNNAC 6 cut(s) 54, 232, 506, 544, 960, 1018
Hpy188I TCNGA 4 cut(s) 75, 781, 1057, 1145
Hpy188III TCNNGA 4 cut(s) 97, 410, 631, 797
Hpy8I GTNNAC 6 cut(s) 54, 232, 506, 544, 960, 1018
Hpy99I CGWCG 1 cut(s) 961
HpyAV CCTTC 6 cut(s) 519, 1189, 1195, 1258, 1291, 1539
HpyCH4III ACNGT 7 cut(s) 503, 622, 704, 754, 775, 879, 1083
HpyCH4IV ACGT 1 cut(s) 1308
HpyCH4V TGCA 7 cut(s) 506, 563, 809, 833, 1079, 1259, 1358
HpySE526I ACGT 1 cut(s) 1308
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 1 cut(s) 317
HspAI GCGC 2 cut(s) 242, 287
KasI GGCGCC 1 cut(s) 286
KpnI GGTACC 1 cut(s) 1433
Kzo9I GATC 4 cut(s) 99, 121, 647, 1145
LmnI GCTCC 3 cut(s) 1354, 1394, 1468
Lsp1109I GCAGC 3 cut(s) 617, 772, 1280
LweI GCATC 6 cut(s) 403, 696, 772, 822, 854, 1327
MaeI CTAG 3 cut(s) 711, 1334, 1376
MaeII ACGT 1 cut(s) 1308
MaeIII GTNAC 5 cut(s) 297, 309, 424, 622, 1318
MalI GATC 4 cut(s) 101, 123, 649, 1147
MboI GATC 4 cut(s) 99, 121, 647, 1145
MboII GAAGA 1 cut(s) 1336
MhlI GDGCHC 1 cut(s) 508
MlsI TGGCCA 2 cut(s) 534, 1212
MluNI TGGCCA 2 cut(s) 534, 1212
Mly113I GGCGCC 1 cut(s) 287
MlyI GAGTC 3 cut(s) 64, 192, 955
MmeI TCCRAC 3 cut(s) 151, 497, 522
Mox20I TGGCCA 2 cut(s) 534, 1212
MscI TGGCCA 2 cut(s) 534, 1212
MseI TTAA 4 cut(s) 936, 1119, 1170, 1277
MslI CAYNNNNRTG 2 cut(s) 227, 1041
Msp20I TGGCCA 2 cut(s) 534, 1212
MspA1I CMGCKG 3 cut(s) 374, 602, 1088
MspCI CTTAAG 1 cut(s) 1118
MspI CCGG 6 cut(s) 88, 236, 444, 513, 945, 1394
MspR9I CCNGG 4 cut(s) 236, 445, 1394, 1395
Mva1269I GAATGC 1 cut(s) 445
MvnI CGCG 2 cut(s) 242, 296
NarI GGCGCC 1 cut(s) 287
NciI CCSGG 4 cut(s) 236, 445, 1394, 1395
NdeI CATATG 1 cut(s) 1492
NdeII GATC 4 cut(s) 99, 121, 647, 1145
NheI GCTAGC 1 cut(s) 1375
NlaIII CATG 1 cut(s) 317
NlaIV GGNNCC 6 cut(s) 288, 485, 1350, 1390, 1431, 1470
NmuCI GTSAC 4 cut(s) 297, 424, 622, 1318
PceI AGGCCT 1 cut(s) 1218
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PctI GAATGC 1 cut(s) 445
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 6 cut(s) 575, 604, 607, 787, 1270, 1388
PleI GAGTC 3 cut(s) 64, 192, 955
PluTI GGCGCC 1 cut(s) 290
PpsI GAGTC 3 cut(s) 64, 192, 955
Ppu21I YACGTR 1 cut(s) 1309
PsiI TTATAA 1 cut(s) 672
PspFI CCCAGC 1 cut(s) 1408
PspN4I GGNNCC 6 cut(s) 288, 485, 1350, 1390, 1431, 1470
PspPI GGNCC 4 cut(s) 85, 483, 495, 758
PstNI CAGNNNCTG 1 cut(s) 785
PvuII CAGCTG 2 cut(s) 374, 1088
RsaI GTAC 2 cut(s) 854, 1431
RsaNI GTAC 2 cut(s) 853, 1430
RseI CAYNNNNRTG 2 cut(s) 227, 1041
SalI GTCGAC 1 cut(s) 958
SaqAI TTAA 4 cut(s) 936, 1119, 1170, 1277
SatI GCNGC 6 cut(s) 574, 603, 606, 786, 1269, 1387
Sau3AI GATC 4 cut(s) 99, 121, 647, 1145
Sau96I GGNCC 4 cut(s) 85, 483, 495, 758
SchI GAGTC 3 cut(s) 64, 192, 955
ScrFI CCNGG 4 cut(s) 236, 445, 1394, 1395
SduI GDGCHC 1 cut(s) 508
SfaNI GCATC 6 cut(s) 403, 696, 772, 822, 854, 1327
SfoI GGCGCC 1 cut(s) 288
SinI GGWCC 2 cut(s) 483, 495
SmaI CCCGGG 1 cut(s) 1395
SmiMI CAYNNNNRTG 2 cut(s) 227, 1041
SmlI CTYRAG 3 cut(s) 157, 577, 1118
SmoI CTYRAG 3 cut(s) 157, 577, 1118
SpeI ACTAGT 1 cut(s) 710
SseBI AGGCCT 1 cut(s) 1218
SspDI GGCGCC 1 cut(s) 286
SspMI CTAG 3 cut(s) 711, 1334, 1376
StuI AGGCCT 1 cut(s) 1218
StyD4I CCNGG 4 cut(s) 234, 443, 1392, 1393
StyI CCWWGG 6 cut(s) 181, 351, 535, 861, 1149, 1213
TaaI ACNGT 7 cut(s) 503, 622, 704, 754, 775, 879, 1083
TaiI ACGT 1 cut(s) 1311
TaqI TCGA 5 cut(s) 98, 715, 816, 848, 959
TauI GCSGC 3 cut(s) 576, 605, 1389
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 4 cut(s) 936, 1119, 1170, 1277
Tru9I TTAA 4 cut(s) 936, 1119, 1170, 1277
TscAI CASTG 2 cut(s) 627, 1086
TseFI GTSAC 4 cut(s) 297, 424, 622, 1318
TseI GCWGC 3 cut(s) 605, 785, 1268
Tsp45I GTSAC 4 cut(s) 297, 424, 622, 1318
TspDTI ATGAA 3 cut(s) 285, 987, 1059
TspMI CCCGGG 1 cut(s) 1393
TspRI CASTG 2 cut(s) 627, 1086
Vha464I CTTAAG 1 cut(s) 1118
VneI GTGCAC 1 cut(s) 504
VpaK11BI GGWCC 2 cut(s) 483, 495
XagI CCTNNNNNAGG 1 cut(s) 743
XapI RAATTY 3 cut(s) 92, 1279, 1312
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XmaI CCCGGG 1 cut(s) 1393
XmiI GTMKAC 1 cut(s) 959
XspI CTAG 3 cut(s) 711, 1334, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.