Rh6BG020500

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000427
Physical Location & Seq
Reverse (-)
3060611 .. 3062907
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG020500.1

Sequence Viewer

Length: 1560 bp
ATGGCTACAAAACCACCATCATGCTTTTCTCTCTTCTCAGTATTGGTGTTGGTTCTTCTAGCCTTAACATCATTTGGGTCCGAAAGTTTCACTGTCCATGGGTTGTCAATTAGAGAAGCTACAATCTACGATCTTCAACTGGCTTTCAAACAGAACCAACTAACTTCCAGACAAGTAGTTCAGTTCTACCTTCAAGAAATCCGCAGGCTCAACCCGGTTCTTAACGGAGTCATAGAGGTGAACCCGGATGCACTTTACCAAGCTGACAAGGCTGACTATCAGCGAAAGGCTAAGGCCCCTGGTTATTATTCCGGCTTGCATGGCATTCCTGTTTTGTTGAAGGATAACATTGGAACCAGAGATAAGCTGAACACTACTGCTGGCTCTTTTGCGCTGCTCGGCTCTGTCGTGCCCCGGGATGCTGGTGTGGTGACCAAGTTGAGGAGCGCTGGAGCCATTATCTTGGGGAAGGCTAGCTTGAGTGAGTGGGCTCAGGTCAGGTCTCTTACGTCACCCCCAGGTTTCAGCCCCAGAGGTGGCCAAGGAAAGAACCCTTATGTACTATCTGCAAGTCCATGTGGATCAAGCAGTGGATCATCAATATCAGTTGCAGCAAATTTGGTAGCTGTATCTCTTGGTACTGAGACCGATGGCTCAATACTATGTCCAGCAAGCTTTAACTCAGTAGTTGGCATCAAACCAACCGTTGGACTCACAAGCCGAGCCGGGGTGATCCCAGTTACTCCCAGACAGGACACCATTGGACCCATCTGCAGGACTGTGACAGATGCTGTTTATGTTCTTGATGCGATTGTAGGCTATGACTACAAGGATCAAGCAACCAAAGAAGCATCGAAATACATTCCACGCGGTGGGTATAAGCAGTTCCTTGGGGCTTATGGGCTCAAGGGAAAGAGACTGGGAATAGTGAGGAATCCCTTCTTCACCTCTGGGAGTGGATCTCTCCAAATTCAAGCATTTGAGCATCATTTCCAAACGCTAAGGCAAGGAGGTGCTGTTTTGGTAGACCATCTGGAAATAGCAAACATTGATACTATCTTGAATTTTAATTTGAGTGGAGAAGCAATAGCAACAGTGGCTGAGTTCAAACTAGCCATAAATGCATATCTAGAAGACCTTGTGGTGTCCCCAGTTAGATCTTTGGCAGATATCATAGCCTTCAACTCGAAGTTTTCTGGTGTGGAAATGATCAAGGAATTTGGCCAAGACATTTTTCTTGCAGCACAAGCCACAAATGGGATAGGCAACAATGAGAAGGCAGCATTGTTAAATTTAGCAAAGCTGACAAAAGATGGATTTGAGAAACTAATGACATACAACAGGCTAGATGCATTGGTGACTCCCGGTGCAGATATTGCTCCTGTACTTGCAATTGGGGGATTCCCAGGCATTAGCGTCCCTGCTGGATATGACAAGAAGGGTGTGCCTTTCGGCATAACTTTTGGGGGTCTAAAGGGATCTGAACCAAAGCTTATACAGATTGCCTACGGTTTCGAGCAAGCTACTGACATTAGAAAGCCTCCCACATTTCTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

519

Amino Acids

55.17

Weight (kDa)

9.07

Isoelectric Point (pI)

29.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 59 - 462 1.9e-71 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 707, 872
AccI GTMKAC 1 cut(s) 1026
AccII CGCG 1 cut(s) 870
AciI CCGC 2 cut(s) 202, 870
AclWI GGATC 6 cut(s) 589, 601, 727, 840, 967, 1486
AcoI YGGCCR 2 cut(s) 538, 1222
AcsI RAATTY 5 cut(s) 616, 969, 1063, 1217, 1291
AdeI CACNNNGTG 1 cut(s) 872
AfaI GTAC 3 cut(s) 561, 640, 1386
AfeI AGCGCT 1 cut(s) 448
AfiI CCNNNNNNNGG 7 cut(s) 441, 536, 707, 727, 774, 872, 1257
AgsI TTSAA 8 cut(s) 137, 148, 194, 340, 974, 1063, 1108, 1183
AjnI CCWGG 3 cut(s) 298, 517, 1405
AjuI GAANNNNNNNTTGG 2 cut(s) 1218, 1250
AluBI AGCT 9 cut(s) 119, 263, 367, 477, 626, 675, 1303, 1492, 1523
AluI AGCT 9 cut(s) 119, 263, 367, 477, 626, 675, 1303, 1492, 1523
Alw26I GTCTC 3 cut(s) 507, 638, 910
AlwI GGATC 6 cut(s) 589, 601, 727, 840, 967, 1486
AlwNI CAGNNNCTG 2 cut(s) 791, 1100
Ama87I CYCGRG 1 cut(s) 414
Aor51HI AGCGCT 1 cut(s) 448
AoxI GGCC 3 cut(s) 294, 538, 1222
ApeKI GCWGC 4 cut(s) 394, 611, 1241, 1280
ApoI RAATTY 5 cut(s) 616, 969, 1063, 1217, 1291
Asp700I GAANNNNTTC 1 cut(s) 938
AspLEI GCGC 2 cut(s) 394, 449
AspS9I GGNCC 3 cut(s) 78, 295, 764
AsuC2I CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1365
AsuHPI GGTGA 6 cut(s) 250, 442, 504, 742, 937, 1369
AsuNHI GCTAGC 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 2 cut(s) 78, 764
BaeGI GKGCMC 1 cut(s) 414
BalI TGGCCA 2 cut(s) 540, 1224
BanII GRGCYC 2 cut(s) 493, 906
BbsI GAAGAC 1 cut(s) 1140
BbvI GCAGC 4 cut(s) 381, 623, 1253, 1292
BccI CCATC 5 cut(s) 25, 644, 776, 1038, 1307
BciT130I CCWGG 3 cut(s) 300, 519, 1407
BclI TGATCA 1 cut(s) 1209
BcnI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1365
BcoDI GTCTC 3 cut(s) 507, 638, 910
BfaI CTAG 5 cut(s) 59, 474, 1112, 1130, 1346
BfmI CTRYAG 1 cut(s) 772
BfoI RGCGCY 1 cut(s) 450
BglII AGATCT 1 cut(s) 1157
BisI GCNGC 4 cut(s) 395, 612, 1242, 1281
BlsI GCNGC 4 cut(s) 396, 613, 1243, 1282
Bme1390I CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1365, 1407
Bme18I GGWCC 2 cut(s) 78, 764
BmeT110I CYCGRG 1 cut(s) 414
BmgT120I GGNCC 3 cut(s) 78, 295, 764
BmiI GGNNCC 5 cut(s) 79, 297, 355, 454, 766
BmrFI CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1365, 1407
BmrI ACTGGG 3 cut(s) 731, 929, 1145
BmsI GCATC 8 cut(s) 238, 409, 702, 778, 796, 860, 994, 1339
BmtI GCTAGC 1 cut(s) 477
BmuI ACTGGG 3 cut(s) 731, 929, 1145
BpiI GAAGAC 1 cut(s) 1140
BplI GAGNNNNNCTC 2 cut(s) 946, 978
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 3 cut(s) 291, 492, 1001
BpuEI CTTGAG 2 cut(s) 499, 890
BpuMI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1365
BsaI GGTCTC 2 cut(s) 507, 638
BsaJI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 889, 1405
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 7 cut(s) 441, 536, 707, 727, 774, 872, 1257
Bse1I ACTGG 4 cut(s) 144, 737, 924, 1151
BseBI CCWGG 3 cut(s) 300, 519, 1407
BseDI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 889, 1405
BseGI GGATG 2 cut(s) 253, 424
BseLI CCNNNNNNNGG 7 cut(s) 441, 536, 707, 727, 774, 872, 1257
BseMII CTCAG 5 cut(s) 51, 506, 633, 696, 1092
BseNI ACTGG 4 cut(s) 144, 737, 924, 1151
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 414
BseXI GCAGC 4 cut(s) 381, 623, 1253, 1292
BsgI GTGCAG 1 cut(s) 1389
Bsh1236I CGCG 1 cut(s) 870
BshFI GGCC 3 cut(s) 296, 540, 1224
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 6 cut(s) 215, 245, 312, 415, 726, 1365
BslFI GGGAC 2 cut(s) 1132, 1403
BslI CCNNNNNNNGG 7 cut(s) 441, 536, 707, 727, 774, 872, 1257
BsmAI GTCTC 3 cut(s) 507, 638, 910
BsmFI GGGAC 2 cut(s) 1132, 1403
BsmI GAATGC 1 cut(s) 324
BsnI GGCC 3 cut(s) 296, 540, 1224
Bso31I GGTCTC 2 cut(s) 507, 638
BsoBI CYCGRG 1 cut(s) 414
Bsp1286I GDGCHC 3 cut(s) 414, 493, 906
Bsp143I GATC 9 cut(s) 130, 581, 593, 732, 832, 959, 1157, 1209, 1478
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 2 cut(s) 202, 870
BspANI GGCC 3 cut(s) 296, 540, 1224
BspCNI CTCAG 5 cut(s) 50, 505, 634, 695, 1093
BspFNI CGCG 1 cut(s) 870
BspLI GGNNCC 5 cut(s) 79, 297, 355, 454, 766
BspMAI CTGCAG 1 cut(s) 776
BspOI GCTAGC 1 cut(s) 477
BspPI GGATC 6 cut(s) 589, 601, 727, 840, 967, 1486
BspTNI GGTCTC 2 cut(s) 507, 638
BsrI ACTGG 4 cut(s) 144, 737, 924, 1151
BssECI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 889, 1405
BssMI GATC 9 cut(s) 130, 581, 593, 732, 832, 959, 1157, 1209, 1478
BssT1I CCWWGG 3 cut(s) 97, 541, 889
Bst2UI CCWGG 3 cut(s) 300, 519, 1407
Bst4CI ACNGT 5 cut(s) 94, 706, 781, 1096, 1511
Bst6I CTCTTC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 1376
BstC8I GCNNGC 6 cut(s) 206, 317, 382, 475, 673, 1521
BstDEI CTNAG 7 cut(s) 37, 291, 492, 642, 682, 1001, 1101
BstDSI CCRYGG 1 cut(s) 97
BstEII GGTNACC 1 cut(s) 430
BstF5I GGATG 2 cut(s) 253, 424
BstFNI CGCG 1 cut(s) 870
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 2 cut(s) 394, 449
BstKTI GATC 9 cut(s) 133, 584, 596, 735, 835, 962, 1160, 1212, 1481
BstMAI GTCTC 3 cut(s) 507, 638, 910
BstMBI GATC 9 cut(s) 130, 581, 593, 732, 832, 959, 1157, 1209, 1478
BstMWI GCNNNNNNNGC 6 cut(s) 269, 321, 1097, 1121, 1247, 1376
BstNI CCWGG 3 cut(s) 300, 519, 1407
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 9 cut(s) 213, 243, 298, 413, 414, 517, 725, 1363, 1405
BstSFI CTRYAG 1 cut(s) 772
BstSLI GKGCMC 1 cut(s) 414
BstUI CGCG 1 cut(s) 870
BstV1I GCAGC 4 cut(s) 381, 623, 1253, 1292
BstV2I GAAGAC 1 cut(s) 1140
BstX2I RGATCY 3 cut(s) 959, 1157, 1478
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 3 cut(s) 959, 1157, 1478
BsuRI GGCC 3 cut(s) 296, 540, 1224
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 2 cut(s) 253, 424
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 3 cut(s) 90, 595, 1101
Cac8I GCNNGC 6 cut(s) 206, 317, 382, 475, 673, 1521
CaiI CAGNNNCTG 2 cut(s) 791, 1100
CfoI GCGC 2 cut(s) 394, 449
Cfr13I GGNCC 3 cut(s) 78, 295, 764
Cfr9I CCCGGG 1 cut(s) 414
CseI GACGC 1 cut(s) 1405
Csp6I GTAC 3 cut(s) 560, 639, 1385
CviAII CATG 4 cut(s) 21, 98, 320, 576
CviQI GTAC 3 cut(s) 560, 639, 1385
DdeI CTNAG 7 cut(s) 37, 291, 492, 642, 682, 1001, 1101
DpnI GATC 9 cut(s) 132, 583, 595, 734, 834, 961, 1159, 1211, 1480
DpnII GATC 9 cut(s) 130, 581, 593, 732, 832, 959, 1157, 1209, 1478
DraIII CACNNNGTG 1 cut(s) 872
EaeI YGGCCR 2 cut(s) 538, 1222
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 3 cut(s) 97, 541, 889
Eco24I GRGCYC 2 cut(s) 493, 906
Eco31I GGTCTC 2 cut(s) 507, 638
Eco32I GATATC 1 cut(s) 1171
Eco47I GGWCC 2 cut(s) 78, 764
Eco47III AGCGCT 1 cut(s) 448
Eco88I CYCGRG 1 cut(s) 414
Eco91I GGTNACC 1 cut(s) 430
EcoO109I RGGNCCY 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 430
EcoRII CCWGG 3 cut(s) 298, 517, 1405
EcoRV GATATC 1 cut(s) 1171
EcoT14I CCWWGG 3 cut(s) 97, 541, 889
EcoT22I ATGCAT 2 cut(s) 1126, 1354
EcoT38I GRGCYC 2 cut(s) 493, 906
ErhI CCWWGG 3 cut(s) 97, 541, 889
FaeI CATG 4 cut(s) 24, 101, 323, 579
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 2 cut(s) 1132, 1403
FatI CATG 4 cut(s) 20, 97, 319, 575
FbaI TGATCA 1 cut(s) 1209
FblI GTMKAC 1 cut(s) 1026
Fnu4HI GCNGC 4 cut(s) 395, 612, 1242, 1281
FokI GGATG 2 cut(s) 260, 431
FriOI GRGCYC 2 cut(s) 493, 906
Fsp4HI GCNGC 4 cut(s) 395, 612, 1242, 1281
FspBI CTAG 5 cut(s) 59, 474, 1112, 1130, 1346
GlaI GCGC 2 cut(s) 393, 448
GluI GCNGC 4 cut(s) 395, 612, 1242, 1281
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 450
HaeIII GGCC 3 cut(s) 296, 540, 1224
HapII CCGG 6 cut(s) 215, 245, 312, 415, 726, 1365
HgaI GACGC 1 cut(s) 1405
HhaI GCGC 2 cut(s) 394, 449
Hin1II CATG 4 cut(s) 24, 101, 323, 579
Hin6I GCGC 2 cut(s) 392, 447
HinP1I GCGC 2 cut(s) 392, 447
HindIII AAGCTT 2 cut(s) 673, 1490
HinfI GANTC 5 cut(s) 228, 711, 934, 1360, 1401
HpaII CCGG 6 cut(s) 215, 245, 312, 415, 726, 1365
HphI GGTGA 6 cut(s) 250, 442, 504, 742, 937, 1369
Hpy166II GTNNAC 2 cut(s) 241, 1027
Hpy188I TCNGA 2 cut(s) 82, 1483
Hpy188III TCNNGA 6 cut(s) 168, 194, 803, 1034, 1060, 1130
Hpy8I GTNNAC 2 cut(s) 241, 1027
HpyAV CCTTC 7 cut(s) 200, 334, 463, 949, 1189, 1270, 1432
HpyCH4III ACNGT 5 cut(s) 94, 706, 781, 1096, 1511
HpyCH4IV ACGT 1 cut(s) 509
HpyF10VI GCNNNNNNNGC 6 cut(s) 269, 321, 1097, 1121, 1247, 1376
HpyF3I CTNAG 7 cut(s) 37, 291, 492, 642, 682, 1001, 1101
HpySE526I ACGT 1 cut(s) 509
Hsp92II CATG 4 cut(s) 24, 101, 323, 579
HspAI GCGC 2 cut(s) 392, 447
Ksp22I TGATCA 1 cut(s) 1209
Kzo9I GATC 9 cut(s) 130, 581, 593, 732, 832, 959, 1157, 1209, 1478
LmnI GCTCC 3 cut(s) 444, 452, 1384
Lsp1109I GCAGC 4 cut(s) 381, 623, 1253, 1292
LweI GCATC 8 cut(s) 238, 409, 702, 778, 796, 860, 994, 1339
MaeI CTAG 5 cut(s) 59, 474, 1112, 1130, 1346
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 5 cut(s) 430, 510, 739, 781, 1357
MalI GATC 9 cut(s) 132, 583, 595, 734, 834, 961, 1159, 1211, 1480
MboI GATC 9 cut(s) 130, 581, 593, 732, 832, 959, 1157, 1209, 1478
MboII GAAGA 5 cut(s) 25, 47, 125, 934, 1145
MfeI CAATTG 1 cut(s) 1392
MflI RGATCY 3 cut(s) 959, 1157, 1478
MhlI GDGCHC 3 cut(s) 414, 493, 906
MlsI TGGCCA 2 cut(s) 540, 1224
MluCI AATT 8 cut(s) 108, 616, 969, 1063, 1069, 1217, 1291, 1392
MluNI TGGCCA 2 cut(s) 540, 1224
MlyI GAGTC 3 cut(s) 237, 705, 1354
MmeI TCCRAC 1 cut(s) 688
MnlI CCTC 7 cut(s) 229, 435, 527, 924, 958, 1004, 1551
Mox20I TGGCCA 2 cut(s) 540, 1224
Mph1103I ATGCAT 2 cut(s) 1126, 1354
MroXI GAANNNNTTC 1 cut(s) 938
MscI TGGCCA 2 cut(s) 540, 1224
MseI TTAA 5 cut(s) 65, 222, 678, 1068, 1289
MslI CAYNNNNRTG 2 cut(s) 19, 236
Msp20I TGGCCA 2 cut(s) 540, 1224
MspI CCGG 6 cut(s) 215, 245, 312, 415, 726, 1365
MspR9I CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1365, 1407
MunI CAATTG 1 cut(s) 1392
Mva1269I GAATGC 1 cut(s) 324
MvaI CCWGG 3 cut(s) 300, 519, 1407
MvnI CGCG 1 cut(s) 870
MwoI GCNNNNNNNGC 6 cut(s) 269, 321, 1097, 1121, 1247, 1376
NciI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1365
NcoI CCATGG 1 cut(s) 97
NdeII GATC 9 cut(s) 130, 581, 593, 732, 832, 959, 1157, 1209, 1478
NheI GCTAGC 1 cut(s) 473
NlaIII CATG 4 cut(s) 24, 101, 323, 579
NlaIV GGNNCC 5 cut(s) 79, 297, 355, 454, 766
NmeAIII GCCGAG 2 cut(s) 378, 746
NmuCI GTSAC 4 cut(s) 430, 510, 781, 1357
NsiI ATGCAT 2 cut(s) 1126, 1354
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PctI GAATGC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 938
PfeI GAWTC 2 cut(s) 934, 1401
PflMI CCANNNNNTGG 2 cut(s) 707, 872
PkrI GCNGC 4 cut(s) 396, 613, 1243, 1282
PleI GAGTC 3 cut(s) 236, 705, 1354
PpsI GAGTC 3 cut(s) 236, 705, 1354
Psp6I CCWGG 3 cut(s) 298, 517, 1405
PspEI GGTNACC 1 cut(s) 430
PspGI CCWGG 3 cut(s) 298, 517, 1405
PspN4I GGNNCC 5 cut(s) 79, 297, 355, 454, 766
PspPI GGNCC 3 cut(s) 78, 295, 764
PsrI GAACNNNNNNTAC 2 cut(s) 869, 901
PstI CTGCAG 1 cut(s) 776
PstNI CAGNNNCTG 2 cut(s) 791, 1100
PsuI RGATCY 3 cut(s) 959, 1157, 1478
RsaI GTAC 3 cut(s) 561, 640, 1386
RsaNI GTAC 3 cut(s) 560, 639, 1385
RseI CAYNNNNRTG 2 cut(s) 19, 236
SaqAI TTAA 5 cut(s) 65, 222, 678, 1068, 1289
SatI GCNGC 4 cut(s) 395, 612, 1242, 1281
Sau3AI GATC 9 cut(s) 130, 581, 593, 732, 832, 959, 1157, 1209, 1478
Sau96I GGNCC 3 cut(s) 78, 295, 764
SchI GAGTC 3 cut(s) 237, 705, 1354
ScrFI CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1365, 1407
SduI GDGCHC 3 cut(s) 414, 493, 906
SfaNI GCATC 8 cut(s) 238, 409, 702, 778, 796, 860, 994, 1339
SfcI CTRYAG 1 cut(s) 772
SinI GGWCC 2 cut(s) 78, 764
SmaI CCCGGG 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 19, 236
SmlI CTYRAG 2 cut(s) 478, 905
SmoI CTYRAG 2 cut(s) 478, 905
Sse9I AATT 8 cut(s) 108, 616, 969, 1063, 1069, 1217, 1291, 1392
SsiI CCGC 2 cut(s) 202, 870
SspMI CTAG 5 cut(s) 59, 474, 1112, 1130, 1346
StyD4I CCNGG 9 cut(s) 213, 243, 298, 413, 414, 517, 725, 1363, 1405
StyI CCWWGG 3 cut(s) 97, 541, 889
TaaI ACNGT 5 cut(s) 94, 706, 781, 1096, 1511
TaiI ACGT 1 cut(s) 512
TaqI TCGA 3 cut(s) 854, 1187, 1515
TaqII GACCGA 1 cut(s) 662
TasI AATT 8 cut(s) 108, 616, 969, 1063, 1069, 1217, 1291, 1392
TatI WGTACW 2 cut(s) 559, 1384
TfiI GAWTC 2 cut(s) 934, 1401
Tru1I TTAA 5 cut(s) 65, 222, 678, 1068, 1289
Tru9I TTAA 5 cut(s) 65, 222, 678, 1068, 1289
TscAI CASTG 3 cut(s) 97, 595, 1101
TseFI GTSAC 4 cut(s) 430, 510, 781, 1357
TseI GCWGC 4 cut(s) 394, 611, 1241, 1280
Tsp45I GTSAC 4 cut(s) 430, 510, 781, 1357
TspGWI ACGGA 1 cut(s) 240
TspMI CCCGGG 1 cut(s) 414
TspRI CASTG 3 cut(s) 97, 595, 1101
Van91I CCANNNNNTGG 2 cut(s) 707, 872
VpaK11BI GGWCC 2 cut(s) 78, 764
XapI RAATTY 5 cut(s) 616, 969, 1063, 1217, 1291
XbaI TCTAGA 1 cut(s) 1129
XmaI CCCGGG 1 cut(s) 414
XmiI GTMKAC 1 cut(s) 1026
XmnI GAANNNNTTC 1 cut(s) 938
XspI CTAG 5 cut(s) 59, 474, 1112, 1130, 1346
Zsp2I ATGCAT 2 cut(s) 1126, 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.