Rw6G005110

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000427
Physical Location & Seq
Forward (+)
8326982 .. 8330076
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G005110.1

Sequence Viewer

Length: 1515 bp
ATGGCTGATAGTAGTCTATTGGGGGGCAATAGACGCACATTCGCCGCCCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTTAAGCACAACCAACTCACCTCGAGGCAACTTGTCCAATATTACCTTTCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAACCCGGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCTACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCACTGCTTGGATCTGTCGTGCCTCGTGATGCCGGCGTGGTGGAGAAGTTGAGGAAAGCTGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCATATTTTAGGTCTAATAATACAGCACCAAATGGTTGGAGCGCCAGAGGCGGTTATGGTTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTAGCCGCAAATCTGGCAGCGGTGTCATTAGGTACTGAGACAGATGGTTCAATCTTATGTCCATCAGGTTACAACTCGGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGAGTTGTCCCAATTTCTCCAAGGCAGGATACTGTTGGACCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATAGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTGGAAATTACTAATTTGACTGAAATCTATTCTTCAAGCAATGAAGATACTGCATTGTCTGTTGAGTTCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCCGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACGTATTTTTAGCTGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCCGGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGACTTGTGAAATTGGTGACAGAGAAGAACCTAGATGCTGTGGTGACTCCTCACGTAGGTGTTTCAAGAGTACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCCGGATACGAAAACAATGGGAGACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCTACTAAGATTAGGAAGCCTCCTTCATTCAAAGGCTTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

504

Amino Acids

54.16

Weight (kDa)

9.49

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 43 - 437 1e-69 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 118
AccB1I GGYRCC 1 cut(s) 247
AccI GTMKAC 1 cut(s) 978
AccII CGCG 1 cut(s) 852
AciI CCGC 8 cut(s) 45, 49, 333, 495, 534, 546, 567, 581
AclWI GGATC 2 cut(s) 364, 1098
AcuI CTGAAG 1 cut(s) 1212
AfaI GTAC 3 cut(s) 595, 815, 1332
AfiI CCNNNNNNNGG 4 cut(s) 662, 827, 1147, 1316
AhlI ACTAGT 1 cut(s) 671
AjnI CCWGG 1 cut(s) 1351
AleI CACNNNNGTG 1 cut(s) 1317
AluBI AGCT 7 cut(s) 91, 404, 432, 1187, 1397, 1438, 1469
AluI AGCT 7 cut(s) 91, 404, 432, 1187, 1397, 1438, 1469
Alw26I GTCTC 3 cut(s) 593, 797, 1384
AlwI GGATC 2 cut(s) 364, 1098
AlwNI CAGNNNCTG 1 cut(s) 746
Ama87I CYCGRG 1 cut(s) 118
ApeKI GCWGC 4 cut(s) 543, 578, 746, 1187
AspLEI GCGC 4 cut(s) 205, 237, 488, 852
AspS9I GGNCC 2 cut(s) 240, 719
AsuC2I CCSGG 2 cut(s) 167, 197
AsuHPI GGTGA 4 cut(s) 106, 200, 1288, 1315
AsuNHI GCTAGC 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 2 cut(s) 240, 719
BanI GGYRCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 1351
BauI CACGAG 1 cut(s) 369
BbvI GCAGC 4 cut(s) 555, 590, 733, 1174
BccI CCATC 2 cut(s) 599, 631
BciT130I CCWGG 1 cut(s) 1353
BciVI GTATCC 2 cut(s) 703, 1367
BcnI CCSGG 2 cut(s) 167, 197
BcoDI GTCTC 3 cut(s) 593, 797, 1384
BcuI ACTAGT 1 cut(s) 671
BfaI CTAG 4 cut(s) 185, 429, 672, 1292
BfoI RGCGCY 1 cut(s) 489
BfuI GTATCC 2 cut(s) 703, 1367
BglI GCCNNNNNGGC 1 cut(s) 221
BisI GCNGC 7 cut(s) 45, 535, 544, 567, 579, 747, 1188
BlsI GCNGC 7 cut(s) 46, 536, 545, 568, 580, 748, 1189
BmcAI AGTACT 1 cut(s) 1332
Bme1390I CCNGG 3 cut(s) 167, 197, 1353
Bme18I GGWCC 2 cut(s) 240, 719
BmeT110I CYCGRG 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 240, 719
BmiI GGNNCC 2 cut(s) 249, 1348
BmrFI CCNGG 3 cut(s) 167, 197, 1353
BmrI ACTGGG 1 cut(s) 1091
BmsI GCATC 7 cut(s) 190, 364, 657, 733, 751, 783, 1285
BmtI GCTAGC 1 cut(s) 432
BmuI ACTGGG 1 cut(s) 1091
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 2 cut(s) 454, 523
BpuMI CCSGG 2 cut(s) 167, 197
BsaAI YACGTR 1 cut(s) 1315
BsaI GGTCTC 1 cut(s) 1384
BsaJI CCNNGG 6 cut(s) 243, 312, 701, 918, 1107, 1351
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 4 cut(s) 662, 827, 1147, 1316
Bse118I RCCGGY 3 cut(s) 335, 377, 1225
Bse1I ACTGG 3 cut(s) 251, 1097, 1152
Bse3DI GCAATG 4 cut(s) 303, 789, 913, 1030
BseBI CCWGG 1 cut(s) 1353
BseDI CCNNGG 6 cut(s) 243, 312, 701, 918, 1107, 1351
BseGI GGATG 1 cut(s) 205
BseLI CCNNNNNNNGG 4 cut(s) 662, 827, 1147, 1316
BseMI GCAATG 4 cut(s) 303, 789, 913, 1030
BseMII CTCAG 4 cut(s) 537, 588, 859, 944
BseNI ACTGG 3 cut(s) 251, 1097, 1152
BseRI GAGGAG 1 cut(s) 1299
BseXI GCAGC 4 cut(s) 555, 590, 733, 1174
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 1 cut(s) 852
BshNI GGYRCC 1 cut(s) 247
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 6 cut(s) 167, 197, 336, 378, 1226, 1371
BslFI GGGAC 1 cut(s) 674
BslI CCNNNNNNNGG 4 cut(s) 662, 827, 1147, 1316
BsmAI GTCTC 3 cut(s) 593, 797, 1384
BsmFI GGGAC 1 cut(s) 674
BsmI GAATGC 1 cut(s) 279
Bso31I GGTCTC 1 cut(s) 1384
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 1351
Bsp143I GATC 4 cut(s) 82, 356, 1103, 1155
BspACI CCGC 8 cut(s) 45, 49, 333, 495, 534, 546, 567, 581
BspCNI CTCAG 4 cut(s) 536, 589, 858, 945
BspFNI CGCG 1 cut(s) 852
BspLI GGNNCC 2 cut(s) 249, 1348
BspOI GCTAGC 1 cut(s) 432
BspPI GGATC 2 cut(s) 364, 1098
BspT107I GGYRCC 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 1384
BsrDI GCAATG 4 cut(s) 303, 789, 913, 1030
BsrFI RCCGGY 3 cut(s) 335, 377, 1225
BsrI ACTGG 3 cut(s) 251, 1097, 1152
BssAI RCCGGY 3 cut(s) 335, 377, 1225
BssECI CCNNGG 6 cut(s) 243, 312, 701, 918, 1107, 1351
BssMI GATC 4 cut(s) 82, 356, 1103, 1155
BssSI CACGAG 1 cut(s) 369
BssT1I CCWWGG 5 cut(s) 243, 312, 701, 918, 1107
Bst2BI CACGAG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 1353
Bst4CI ACNGT 3 cut(s) 715, 736, 840
BstAPI GCANNNNNTGC 1 cut(s) 746
BstBAI YACGTR 1 cut(s) 1315
BstC8I GCNNGC 8 cut(s) 49, 93, 337, 379, 430, 772, 1227, 1467
BstDEI CTNAG 7 cut(s) 267, 523, 597, 845, 860, 953, 1473
BstENI CCTNNNNNAGG 1 cut(s) 1314
BstF5I GGATG 1 cut(s) 205
BstFNI CGCG 1 cut(s) 852
BstH2I RGCGCY 1 cut(s) 489
BstHHI GCGC 4 cut(s) 205, 237, 488, 852
BstKTI GATC 4 cut(s) 85, 359, 1106, 1158
BstMAI GTCTC 3 cut(s) 593, 797, 1384
BstMBI GATC 4 cut(s) 82, 356, 1103, 1155
BstMWI GCNNNNNNNGC 8 cut(s) 33, 221, 492, 540, 543, 575, 746, 1193
BstNI CCWGG 1 cut(s) 1353
BstSCI CCNGG 3 cut(s) 165, 195, 1351
BstUI CGCG 1 cut(s) 852
BstV1I GCAGC 4 cut(s) 555, 590, 733, 1174
BstX2I RGATCY 1 cut(s) 356
BstXI CCANNNNNNTGG 1 cut(s) 480
BstYI RGATCY 1 cut(s) 356
BsuI GTATCC 2 cut(s) 703, 1367
BtsCI GGATG 1 cut(s) 205
BtsI GCAGTG 1 cut(s) 347
BtsIMutI CAGTG 3 cut(s) 258, 347, 1104
Cac8I GCNNGC 8 cut(s) 49, 93, 337, 379, 430, 772, 1227, 1467
CaiI CAGNNNCTG 1 cut(s) 746
CfoI GCGC 4 cut(s) 205, 237, 488, 852
Cfr10I RCCGGY 3 cut(s) 335, 377, 1225
Cfr13I GGNCC 2 cut(s) 240, 719
CseI GACGC 1 cut(s) 42
Csp6I GTAC 3 cut(s) 594, 814, 1331
CviAII CATG 1 cut(s) 275
CviQI GTAC 3 cut(s) 594, 814, 1331
DdeI CTNAG 7 cut(s) 267, 523, 597, 845, 860, 953, 1473
DpnI GATC 4 cut(s) 84, 358, 1105, 1157
DpnII GATC 4 cut(s) 82, 356, 1103, 1155
Eco130I CCWWGG 5 cut(s) 243, 312, 701, 918, 1107
Eco24I GRGCYC 1 cut(s) 1351
Eco31I GGTCTC 1 cut(s) 1384
Eco47I GGWCC 2 cut(s) 240, 719
Eco57I CTGAAG 1 cut(s) 1212
Eco88I CYCGRG 1 cut(s) 118
EcoNI CCTNNNNNAGG 1 cut(s) 1314
EcoRII CCWGG 1 cut(s) 1351
EcoT14I CCWWGG 5 cut(s) 243, 312, 701, 918, 1107
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 1 cut(s) 1351
ErhI CCWWGG 5 cut(s) 243, 312, 701, 918, 1107
FaeI CATG 1 cut(s) 278
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 674
FatI CATG 1 cut(s) 274
FauI CCCGC 1 cut(s) 56
FauNDI CATATG 1 cut(s) 1453
FblI GTMKAC 1 cut(s) 978
Fnu4HI GCNGC 7 cut(s) 45, 535, 544, 567, 579, 747, 1188
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 1 cut(s) 1351
Fsp4HI GCNGC 7 cut(s) 45, 535, 544, 567, 579, 747, 1188
FspBI CTAG 4 cut(s) 185, 429, 672, 1292
GlaI GCGC 4 cut(s) 204, 236, 487, 851
GluI GCNGC 7 cut(s) 45, 535, 544, 567, 579, 747, 1188
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 489
HapII CCGG 6 cut(s) 167, 197, 336, 378, 1226, 1371
HgaI GACGC 1 cut(s) 42
HhaI GCGC 4 cut(s) 205, 237, 488, 852
Hin1II CATG 1 cut(s) 278
Hin6I GCGC 4 cut(s) 203, 235, 486, 850
HinP1I GCGC 4 cut(s) 203, 235, 486, 850
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 4 cut(s) 54, 180, 1237, 1306
HpaII CCGG 6 cut(s) 167, 197, 336, 378, 1226, 1371
HphI GGTGA 4 cut(s) 106, 200, 1288, 1315
Hpy166II GTNNAC 4 cut(s) 193, 979, 1251, 1415
Hpy188I TCNGA 4 cut(s) 155, 526, 954, 1429
Hpy188III TCNNGA 4 cut(s) 371, 758, 1326, 1444
Hpy8I GTNNAC 4 cut(s) 193, 979, 1251, 1415
Hpy99I CGWCG 1 cut(s) 857
HpyAV CCTTC 4 cut(s) 901, 1072, 1135, 1500
HpyCH4III ACNGT 3 cut(s) 715, 736, 840
HpyCH4IV ACGT 2 cut(s) 1176, 1314
HpyCH4V TGCA 9 cut(s) 274, 347, 794, 836, 1037, 1070, 1244, 1337, 1451
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 221, 492, 540, 543, 575, 746, 1193
HpyF3I CTNAG 7 cut(s) 267, 523, 597, 845, 860, 953, 1473
HpySE526I ACGT 2 cut(s) 1176, 1314
Hsp92II CATG 1 cut(s) 278
HspAI GCGC 4 cut(s) 203, 235, 486, 850
KroI GCCGGC 3 cut(s) 335, 377, 1225
KroNI GCCGGC 3 cut(s) 337, 379, 1227
Kzo9I GATC 4 cut(s) 82, 356, 1103, 1155
LmnI GCTCC 2 cut(s) 483, 1346
Lsp1109I GCAGC 4 cut(s) 555, 590, 733, 1174
LweI GCATC 7 cut(s) 190, 364, 657, 733, 751, 783, 1285
MaeI CTAG 4 cut(s) 185, 429, 672, 1292
MaeII ACGT 2 cut(s) 1176, 1314
MaeIII GTNAC 4 cut(s) 255, 629, 1276, 1303
MalI GATC 4 cut(s) 84, 358, 1105, 1157
MboI GATC 4 cut(s) 82, 356, 1103, 1155
MboII GAAGA 3 cut(s) 1008, 1040, 1297
MfeI CAATTG 1 cut(s) 1338
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 1351
MluCI AATT 7 cut(s) 693, 723, 982, 990, 997, 1271, 1338
MlyI GAGTC 3 cut(s) 48, 189, 1300
MmeI TCCRAC 2 cut(s) 461, 697
Mph1103I ATGCAT 1 cut(s) 1072
MroNI GCCGGC 3 cut(s) 335, 377, 1225
MseI TTAA 2 cut(s) 99, 1064
MslI CAYNNNNRTG 1 cut(s) 1317
MspA1I CMGCKG 3 cut(s) 546, 581, 1397
MspI CCGG 6 cut(s) 167, 197, 336, 378, 1226, 1371
MspR9I CCNGG 3 cut(s) 167, 197, 1353
MunI CAATTG 1 cut(s) 1338
Mva1269I GAATGC 1 cut(s) 279
MvaI CCWGG 1 cut(s) 1353
MvnI CGCG 1 cut(s) 852
MwoI GCNNNNNNNGC 8 cut(s) 33, 221, 492, 540, 543, 575, 746, 1193
NaeI GCCGGC 3 cut(s) 337, 379, 1227
NciI CCSGG 2 cut(s) 167, 197
NdeI CATATG 1 cut(s) 1453
NdeII GATC 4 cut(s) 82, 356, 1103, 1155
NgoMIV GCCGGC 3 cut(s) 335, 377, 1225
NheI GCTAGC 1 cut(s) 428
NlaIII CATG 1 cut(s) 278
NlaIV GGNNCC 2 cut(s) 249, 1348
NmuCI GTSAC 3 cut(s) 255, 1276, 1303
NsiI ATGCAT 1 cut(s) 1072
OliI CACNNNNGTG 1 cut(s) 1317
PaeR7I CTCGAG 1 cut(s) 118
PctI GAATGC 1 cut(s) 279
PdiI GCCGGC 3 cut(s) 337, 379, 1227
PfeI GAWTC 1 cut(s) 1237
PkrI GCNGC 7 cut(s) 46, 536, 545, 568, 580, 748, 1189
PleI GAGTC 3 cut(s) 48, 188, 1300
PpsI GAGTC 3 cut(s) 48, 188, 1300
Ppu21I YACGTR 1 cut(s) 1315
Psp6I CCWGG 1 cut(s) 1351
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 1351
PspN4I GGNNCC 2 cut(s) 249, 1348
PspPI GGNCC 2 cut(s) 240, 719
PspXI VCTCGAGB 1 cut(s) 118
PstNI CAGNNNCTG 1 cut(s) 746
PsuI RGATCY 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 1397
RsaI GTAC 3 cut(s) 595, 815, 1332
RsaNI GTAC 3 cut(s) 594, 814, 1331
RseI CAYNNNNRTG 1 cut(s) 1317
SaqAI TTAA 2 cut(s) 99, 1064
SatI GCNGC 7 cut(s) 45, 535, 544, 567, 579, 747, 1188
Sau3AI GATC 4 cut(s) 82, 356, 1103, 1155
Sau96I GGNCC 2 cut(s) 240, 719
ScaI AGTACT 1 cut(s) 1332
SchI GAGTC 3 cut(s) 48, 189, 1300
ScrFI CCNGG 3 cut(s) 167, 197, 1353
SduI GDGCHC 1 cut(s) 1351
SfaNI GCATC 7 cut(s) 190, 364, 657, 733, 751, 783, 1285
Sfr274I CTCGAG 1 cut(s) 118
SinI GGWCC 2 cut(s) 240, 719
SlaI CTCGAG 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 1317
SmlI CTYRAG 3 cut(s) 118, 433, 538
SmoI CTYRAG 3 cut(s) 118, 433, 538
SpeI ACTAGT 1 cut(s) 671
Sse9I AATT 7 cut(s) 693, 723, 982, 990, 997, 1271, 1338
SsiI CCGC 8 cut(s) 45, 49, 333, 495, 534, 546, 567, 581
SspI AATATT 1 cut(s) 137
SspMI CTAG 4 cut(s) 185, 429, 672, 1292
StyD4I CCNGG 3 cut(s) 165, 195, 1351
StyI CCWWGG 5 cut(s) 243, 312, 701, 918, 1107
TaaI ACNGT 3 cut(s) 715, 736, 840
TaiI ACGT 2 cut(s) 1179, 1317
TaqI TCGA 4 cut(s) 119, 676, 777, 1443
TaqII GACCGA 1 cut(s) 653
TasI AATT 7 cut(s) 693, 723, 982, 990, 997, 1271, 1338
TatI WGTACW 2 cut(s) 813, 1330
TauI GCSGC 3 cut(s) 47, 537, 569
TfiI GAWTC 1 cut(s) 1237
Tru1I TTAA 2 cut(s) 99, 1064
Tru9I TTAA 2 cut(s) 99, 1064
TscAI CASTG 3 cut(s) 258, 354, 1104
TseFI GTSAC 3 cut(s) 255, 1276, 1303
TseI GCWGC 4 cut(s) 543, 578, 746, 1187
Tsp45I GTSAC 3 cut(s) 255, 1276, 1303
TspDTI ATGAA 3 cut(s) 338, 1041, 1482
TspGWI ACGGA 1 cut(s) 870
TspRI CASTG 3 cut(s) 258, 354, 1104
VpaK11BI GGWCC 2 cut(s) 240, 719
XagI CCTNNNNNAGG 1 cut(s) 1314
XhoI CTCGAG 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 978
XspI CTAG 4 cut(s) 185, 429, 672, 1292
ZrmI AGTACT 1 cut(s) 1332
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.