pycom10g10360

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000427
Physical Location & Seq
Forward (+)
13626362 .. 13628025
1664 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g10360.4

Sequence Viewer

Length: 1059 bp
ATGATGCACCTCCTGGTTGGAATGCCAGAGGCGGCCAAGGGGTGGCAGAACCCTTACAATTCTCTTGCAACACCTTGCGGCTCAAGTAGCGGTTCAGCAATATCAGCGGCAGCAAATCTGGCAACAGTGACACTCGGCACTGAAACAGATGGATCAATTTTATGTCCATCCAGTTATAACTCAGTCGTGGGCATCAAACCAACAAACGGTCTTACTAGTCGAGCAGGGGTTGTCCCGGTCTCTCTAAGACAGGACACAGTTGGGCCAATTTGTCGGACAGTATCAGATGCTGCTCATGTTCTTGATGTCATTGCCGGCATCGACCAGAACAATATTGCAACGACTGGGGCATCGAGGTACATTCCCAAGGGTGGCTACGCACGGTTCCTCAGGCGTGATGGGCTTAGAGGAAAGCGAATAGGGATAGTGAGAGCCTTATTTAACTTCTCCGGTCAAACTTCGTTGACTCCAATTTATGAAAAACACTTCAACACGCTGAGGAAAGGTGGTGCTGTTTTGGTAGACAATTTGGAAATACCACATTTTGATGAAATCTATTCCGATGGAAGCAGTGAGAATACTGCACTGTCAGCTGAGTTCAAAATATATATAAACACATACCTTAAGGAGTTGGTGGCTTCGCCAGTCCGATCCTTGGCGGATTTGATAGCCTTTAACAATAAAAACGCAAAACTGGAGAAGGTGAAGGAGTATGGCCAAGGCCTATTTTTAGAAGCTGAAGCAACAAATGGTATAGGAAAGGCAGAGAAGGCAGCATTGTTAAATTTGGCAAGATTCGCGAAGGATGACTACGTGAATTTAGTGACGAAGAACAAGCTAGATGCCGTGGCGGCTCCTCGTCAATACGTGTCGACGTTGCTTGCGATTAGCGGCTCCCCGGGTGTGATTGTCCCAACAGGATATGATGATGGGGTACCAGTTGGCCTTTCCTTTGGAGGGCTGAGGGGTTGGGAGCCAAAGCTAATTGAGATGGCATATGGCTTTGAGCAAGCAACTAGGATTAGGAAGCCTCCTTCACTTAAAAAGTTGAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

353

Amino Acids

37.64

Weight (kDa)

9.23

Isoelectric Point (pI)

28.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
Acc65I GGTACC 1 cut(s) 934
AccB1I GGYRCC 1 cut(s) 934
AccB7I CCANNNNNTGG 1 cut(s) 42
AccI GTMKAC 2 cut(s) 522, 872
AccII CGCG 1 cut(s) 800
AciI CCGC 7 cut(s) 32, 78, 90, 107, 659, 851, 891
AclWI GGATC 2 cut(s) 160, 645
AcoI YGGCCR 2 cut(s) 33, 715
AcsI RAATTY 2 cut(s) 784, 817
AcuI CTGAAG 1 cut(s) 759
AfaI GTAC 2 cut(s) 359, 936
AfiI CCNNNNNNNGG 5 cut(s) 42, 206, 371, 655, 957
AflII CTTAAG 1 cut(s) 623
AflIII ACRYGT 1 cut(s) 867
AgsI TTSAA 3 cut(s) 490, 601, 1051
AhlI ACTAGT 1 cut(s) 215
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 4 cut(s) 593, 737, 838, 982
AluI AGCT 4 cut(s) 593, 737, 838, 982
Alw26I GTCTC 1 cut(s) 244
AlwI GGATC 2 cut(s) 160, 645
AlwNI CAGNNNCTG 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 898
AoxI GGCC 5 cut(s) 33, 263, 715, 721, 943
ApeKI GCWGC 3 cut(s) 110, 290, 773
ApoI RAATTY 2 cut(s) 784, 817
Asp718I GGTACC 1 cut(s) 934
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 3 cut(s) 236, 899, 900
AsuHPI GGTGA 1 cut(s) 715
AvaI CYCGRG 1 cut(s) 898
AxyI CCTNAGG 1 cut(s) 389
BalI TGGCCA 1 cut(s) 717
BanI GGYRCC 1 cut(s) 934
BarI GAAGNNNNNNTAC 2 cut(s) 794, 826
BbvCI CCTCAGC 2 cut(s) 497, 962
BbvI GCAGC 3 cut(s) 122, 277, 785
BccI CCATC 6 cut(s) 143, 175, 392, 557, 923, 985
BceAI ACGGC 1 cut(s) 830
BciT130I CCWGG 1 cut(s) 14
BcnI CCSGG 3 cut(s) 236, 899, 900
BcoDI GTCTC 1 cut(s) 244
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 3 cut(s) 216, 839, 1017
BfrI CTTAAG 1 cut(s) 623
BglI GCCNNNNNGGC 1 cut(s) 851
BisI GCNGC 8 cut(s) 33, 79, 108, 111, 291, 774, 852, 892
BlsI GCNGC 8 cut(s) 34, 80, 109, 112, 292, 775, 853, 893
Bme1390I CCNGG 4 cut(s) 14, 236, 899, 900
BmeT110I CYCGRG 1 cut(s) 898
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 5 cut(s) 386, 855, 895, 936, 975
BmrFI CCNGG 4 cut(s) 14, 236, 899, 900
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 5 cut(s) 201, 277, 327, 359, 832
BmuI ACTGGG 1 cut(s) 354
BpmI CTGGAG 1 cut(s) 716
Bpu10I CCTNAGC 2 cut(s) 497, 962
BpuEI CTTGAG 1 cut(s) 67
BpuMI CCSGG 3 cut(s) 236, 899, 900
BsaAI YACGTR 2 cut(s) 814, 868
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 7 cut(s) 36, 366, 654, 718, 846, 897, 898
BsaWI WCCGGW 1 cut(s) 449
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 206, 371, 655, 957
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 5 cut(s) 171, 349, 644, 699, 938
Bse21I CCTNAGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 7 cut(s) 36, 366, 654, 718, 846, 897, 898
BseGI GGATG 2 cut(s) 167, 811
BseLI CCNNNNNNNGG 5 cut(s) 42, 206, 371, 655, 957
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 5 cut(s) 195, 403, 488, 585, 953
BseNI ACTGG 5 cut(s) 171, 349, 644, 699, 938
BseRI GAGGAG 1 cut(s) 846
BseXI GCAGC 3 cut(s) 122, 277, 785
BsgI GTGCAG 1 cut(s) 567
Bsh1236I CGCG 1 cut(s) 800
BshFI GGCC 5 cut(s) 35, 265, 717, 723, 945
BshNI GGYRCC 1 cut(s) 934
BsiHKCI CYCGRG 1 cut(s) 898
BsiSI CCGG 4 cut(s) 236, 315, 450, 899
BslFI GGGAC 2 cut(s) 218, 896
BslI CCNNNNNNNGG 5 cut(s) 42, 206, 371, 655, 957
BsmAI GTCTC 1 cut(s) 244
BsmFI GGGAC 2 cut(s) 218, 896
BsmI GAATGC 1 cut(s) 27
BsnI GGCC 5 cut(s) 35, 265, 717, 723, 945
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 1 cut(s) 898
Bsp143I GATC 2 cut(s) 152, 650
Bsp68I TCGCGA 1 cut(s) 800
BspACI CCGC 7 cut(s) 32, 78, 90, 107, 659, 851, 891
BspANI GGCC 5 cut(s) 35, 265, 717, 723, 945
BspCNI CTCAG 5 cut(s) 194, 402, 489, 586, 954
BspFNI CGCG 1 cut(s) 800
BspLI GGNNCC 5 cut(s) 386, 855, 895, 936, 975
BspPI GGATC 2 cut(s) 160, 645
BspT107I GGYRCC 1 cut(s) 934
BspTI CTTAAG 1 cut(s) 623
BspTNI GGTCTC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 309
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 5 cut(s) 171, 349, 644, 699, 938
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 7 cut(s) 36, 366, 654, 718, 846, 897, 898
BssMI GATC 2 cut(s) 152, 650
BssT1I CCWWGG 4 cut(s) 36, 366, 654, 718
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 6 cut(s) 127, 209, 259, 280, 384, 588
BstAFI CTTAAG 1 cut(s) 623
BstBAI YACGTR 2 cut(s) 814, 868
BstC8I GCNNGC 3 cut(s) 316, 882, 1011
BstDEI CTNAG 7 cut(s) 181, 245, 389, 404, 497, 594, 962
BstDSI CCRYGG 1 cut(s) 846
BstF5I GGATG 2 cut(s) 167, 811
BstFNI CGCG 1 cut(s) 800
BstKTI GATC 2 cut(s) 155, 653
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 2 cut(s) 152, 650
BstMWI GCNNNNNNNGC 8 cut(s) 87, 104, 119, 400, 590, 770, 797, 851
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 4 cut(s) 12, 234, 897, 898
BstUI CGCG 1 cut(s) 800
BstV1I GCAGC 3 cut(s) 122, 277, 785
Bsu36I CCTNAGG 1 cut(s) 389
BsuRI GGCC 5 cut(s) 35, 265, 717, 723, 945
BtgI CCRYGG 1 cut(s) 846
BtsCI GGATG 2 cut(s) 167, 811
BtsI GCAGTG 1 cut(s) 577
BtsIMutI CAGTG 4 cut(s) 132, 138, 577, 584
BtuMI TCGCGA 1 cut(s) 800
Cac8I GCNNGC 3 cut(s) 316, 882, 1011
CaiI CAGNNNCTG 1 cut(s) 290
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 263
Cfr9I CCCGGG 1 cut(s) 898
Csp6I GTAC 2 cut(s) 358, 935
CviAII CATG 1 cut(s) 296
CviQI GTAC 2 cut(s) 358, 935
DdeI CTNAG 7 cut(s) 181, 245, 389, 404, 497, 594, 962
DpnI GATC 2 cut(s) 154, 652
DpnII GATC 2 cut(s) 152, 650
EaeI YGGCCR 2 cut(s) 33, 715
EciI GGCGGA 1 cut(s) 674
Eco130I CCWWGG 4 cut(s) 36, 366, 654, 718
Eco147I AGGCCT 1 cut(s) 723
Eco31I GGTCTC 1 cut(s) 244
Eco57I CTGAAG 1 cut(s) 759
Eco81I CCTNAGG 1 cut(s) 389
Eco88I CYCGRG 1 cut(s) 898
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 4 cut(s) 36, 366, 654, 718
ErhI CCWWGG 4 cut(s) 36, 366, 654, 718
FaeI CATG 1 cut(s) 299
FaqI GGGAC 2 cut(s) 218, 896
FatI CATG 1 cut(s) 295
FauNDI CATATG 1 cut(s) 997
FblI GTMKAC 2 cut(s) 522, 872
Fnu4HI GCNGC 8 cut(s) 33, 79, 108, 111, 291, 774, 852, 892
FokI GGATG 2 cut(s) 154, 818
Fsp4HI GCNGC 8 cut(s) 33, 79, 108, 111, 291, 774, 852, 892
FspBI CTAG 3 cut(s) 216, 839, 1017
GluI GCNGC 8 cut(s) 33, 79, 108, 111, 291, 774, 852, 892
GsuI CTGGAG 1 cut(s) 716
HaeIII GGCC 5 cut(s) 35, 265, 717, 723, 945
HapII CCGG 4 cut(s) 236, 315, 450, 899
Hin1II CATG 1 cut(s) 299
HincII GTYRAC 2 cut(s) 465, 873
HindII GTYRAC 2 cut(s) 465, 873
HinfI GANTC 2 cut(s) 466, 795
HpaII CCGG 4 cut(s) 236, 315, 450, 899
HphI GGTGA 1 cut(s) 715
Hpy166II GTNNAC 3 cut(s) 465, 523, 873
Hpy188I TCNGA 4 cut(s) 276, 286, 562, 650
Hpy188III TCNNGA 2 cut(s) 302, 799
Hpy8I GTNNAC 3 cut(s) 465, 523, 873
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 5 cut(s) 694, 700, 763, 796, 1044
HpyCH4III ACNGT 6 cut(s) 127, 209, 259, 280, 384, 588
HpyCH4IV ACGT 3 cut(s) 813, 867, 875
HpyCH4V TGCA 4 cut(s) 7, 68, 338, 584
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 104, 119, 400, 590, 770, 797, 851
HpyF3I CTNAG 7 cut(s) 181, 245, 389, 404, 497, 594, 962
HpySE526I ACGT 3 cut(s) 813, 867, 875
Hsp92II CATG 1 cut(s) 299
KpnI GGTACC 1 cut(s) 938
KroI GCCGGC 1 cut(s) 314
KroNI GCCGGC 1 cut(s) 316
Kzo9I GATC 2 cut(s) 152, 650
LmnI GCTCC 3 cut(s) 859, 899, 973
Lsp1109I GCAGC 3 cut(s) 122, 277, 785
LweI GCATC 5 cut(s) 201, 277, 327, 359, 832
MaeI CTAG 3 cut(s) 216, 839, 1017
MaeII ACGT 3 cut(s) 813, 867, 875
MaeIII GTNAC 2 cut(s) 127, 823
MalI GATC 2 cut(s) 154, 652
MboI GATC 2 cut(s) 152, 650
MboII GAAGA 1 cut(s) 841
MlsI TGGCCA 1 cut(s) 717
MluCI AATT 8 cut(s) 58, 156, 267, 471, 526, 784, 817, 984
MluNI TGGCCA 1 cut(s) 717
MlyI GAGTC 1 cut(s) 460
MmeI TCCRAC 1 cut(s) 254
Mox20I TGGCCA 1 cut(s) 717
MroNI GCCGGC 1 cut(s) 314
MscI TGGCCA 1 cut(s) 717
MseI TTAA 5 cut(s) 441, 624, 675, 782, 1041
MslI CAYNNNNRTG 1 cut(s) 546
Msp20I TGGCCA 1 cut(s) 717
MspA1I CMGCKG 2 cut(s) 107, 593
MspCI CTTAAG 1 cut(s) 623
MspI CCGG 4 cut(s) 236, 315, 450, 899
MspR9I CCNGG 4 cut(s) 14, 236, 899, 900
Mva1269I GAATGC 1 cut(s) 27
MvaI CCWGG 1 cut(s) 14
MvnI CGCG 1 cut(s) 800
MwoI GCNNNNNNNGC 8 cut(s) 87, 104, 119, 400, 590, 770, 797, 851
NaeI GCCGGC 1 cut(s) 316
NciI CCSGG 3 cut(s) 236, 899, 900
NdeI CATATG 1 cut(s) 997
NdeII GATC 2 cut(s) 152, 650
NgoMIV GCCGGC 1 cut(s) 314
NlaIII CATG 1 cut(s) 299
NlaIV GGNNCC 5 cut(s) 386, 855, 895, 936, 975
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 2 cut(s) 127, 823
NruI TCGCGA 1 cut(s) 800
PceI AGGCCT 1 cut(s) 723
PcsI WCGNNNNNNNCGW 1 cut(s) 881
PctI GAATGC 1 cut(s) 27
PdiI GCCGGC 1 cut(s) 316
PfeI GAWTC 1 cut(s) 795
PflMI CCANNNNNTGG 1 cut(s) 42
PkrI GCNGC 8 cut(s) 34, 80, 109, 112, 292, 775, 853, 893
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
Ppu21I YACGTR 2 cut(s) 814, 868
PsiI TTATAA 1 cut(s) 177
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 5 cut(s) 386, 855, 895, 936, 975
PspPI GGNCC 1 cut(s) 263
PstNI CAGNNNCTG 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 593
RruI TCGCGA 1 cut(s) 800
RsaI GTAC 2 cut(s) 359, 936
RsaNI GTAC 2 cut(s) 358, 935
RseI CAYNNNNRTG 1 cut(s) 546
SalI GTCGAC 1 cut(s) 871
SaqAI TTAA 5 cut(s) 441, 624, 675, 782, 1041
SatI GCNGC 8 cut(s) 33, 79, 108, 111, 291, 774, 852, 892
Sau3AI GATC 2 cut(s) 152, 650
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 1 cut(s) 460
ScrFI CCNGG 4 cut(s) 14, 236, 899, 900
SfaNI GCATC 5 cut(s) 201, 277, 327, 359, 832
SmaI CCCGGG 1 cut(s) 900
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 2 cut(s) 82, 623
SmoI CTYRAG 2 cut(s) 82, 623
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 8 cut(s) 58, 156, 267, 471, 526, 784, 817, 984
SseBI AGGCCT 1 cut(s) 723
SsiI CCGC 7 cut(s) 32, 78, 90, 107, 659, 851, 891
SspI AATATT 1 cut(s) 334
SspMI CTAG 3 cut(s) 216, 839, 1017
StuI AGGCCT 1 cut(s) 723
StyD4I CCNGG 4 cut(s) 12, 234, 897, 898
StyI CCWWGG 4 cut(s) 36, 366, 654, 718
TaaI ACNGT 6 cut(s) 127, 209, 259, 280, 384, 588
TaiI ACGT 3 cut(s) 816, 870, 878
TaqI TCGA 4 cut(s) 220, 321, 353, 872
TasI AATT 8 cut(s) 58, 156, 267, 471, 526, 784, 817, 984
TauI GCSGC 5 cut(s) 35, 81, 110, 854, 894
TfiI GAWTC 1 cut(s) 795
Tru1I TTAA 5 cut(s) 441, 624, 675, 782, 1041
Tru9I TTAA 5 cut(s) 441, 624, 675, 782, 1041
TscAI CASTG 4 cut(s) 132, 145, 577, 591
TseFI GTSAC 2 cut(s) 127, 823
TseI GCWGC 3 cut(s) 110, 290, 773
Tsp45I GTSAC 2 cut(s) 127, 823
TspDTI ATGAA 2 cut(s) 492, 564
TspMI CCCGGG 1 cut(s) 898
TspRI CASTG 4 cut(s) 132, 145, 577, 591
Van91I CCANNNNNTGG 1 cut(s) 42
Vha464I CTTAAG 1 cut(s) 623
XapI RAATTY 2 cut(s) 784, 817
XmaI CCCGGG 1 cut(s) 898
XmiI GTMKAC 2 cut(s) 522, 872
XspI CTAG 3 cut(s) 216, 839, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.