Rroxscaffold_7G00215630

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000427
Physical Location & Seq
Forward (+)
66221059 .. 66224398
3340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215630.1

Sequence Viewer

Length: 1431 bp
ATGGAAGAAGCCACACTCAATCAAGACCTCAGAGGAGTCTTAGAGATAAACCCAGATGCTTTATTGCAAGCTGACATAGCAGACAAAGAGCGCAGTGAATATGAATCCAGCGCCCAAACACTGCCTAAGCTTCATGGGATTCCTGTTCTGCTCAAGGATAATATAGCAACCAAGGACAAGCTTAACACCACTGCTGGGTCTTATGCTCTTTTGGGTTCCGTAGTGCCTAGAGATGCTGGGGTTGTCTCAAAGTTGAGGAGCTCTGGGGCTATCATTTTGGGGAAGGCTACGTTGAGTGAATGGGCCTATATCAGAACATCTAAAGCACCCTATGGTTGGAGTGCCAGAGGTGGTCAAGGAGTGAATCCTTATAATTCATCACTGGAAGTTTGTGGATCAAGTAGTGGATCCGCAATATCAGTATCTGCAAATATGGTATCAGTGTCTCTAGGAACAGAGACCGATAGCTCCATCTTATGTCCAGCGAGTTTTAACTCGGTAGTGGGCTTCAAACCAACGGTTGGTCTCACTAGTCGAGCAGGAGTCATCCCAGTCAGTCCAAGACAGGACACAATTGGGCCAATTTGTAGGACTGTAGCGGATGCTGTTCATGTCCTTGATACCATTGTTGGCATTGATAGCAATGACAAGGCAACAAATGAAGTAGCCCAGTATATTCCAGTGGGTGGCTATGGCCAGTTTCTCAATTCTAATGGTCTCAAAGGAAAGAGACTGGGGATAGTCAGTCTCATGTTTCTTCAGATCAAGGATGATGCTTTCTTGAATCAAACTTTTGAGCAGCATTTTACCACACTGAGGGAACAAGGTGCAATTTTGGTTGAGAATCTGGAAATAGCCAACCTTGAAGATATTCGCGATTTTACTTCAAGTGGTGAGTTTGTAGCAATGTTAGCTGAATTCAAGATCGCCTTGAATGCGTACCTGGGTGACCTAGTCAAATCTCCAGTGAGAACCTTAGGCCAGGTTATAGCATTCAACAAGAATAATTCTTATCTGGAAAAGAATGATGATTATGGACAATATCTCTTAGAAGCAGCTGAGGAGACTAATGGAATGGGAGCAAAAGAGCTTGAGGCATTGTCAAATATAGAGAGACTATCTAGAGATGGATTTGAGAAGTTGATGACAGATAATAACTTAGATGCATTGGTGACTTATGCTAATAGCGCTTCTTCTATCCTCGCAATTGGTGGTTTCCCAGGCATCGTTGTGCCAGCAGGATACCATGACACTGATAAGTACCCTTTTGGAATTTGCTTTGGGGGATTGAAGGGTTCGGAGCCAAAGCTGATCGAGATTGCCTATGCCTTTGAGCAAGCAACTATGATCAGGAAGCCTCCAACTGTTGATTCTCAGTACCGAAGTATCTCTACCACCGTCACTAGAAGCTCCAACGTAGCTACTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

476

Amino Acids

51.01

Weight (kDa)

4.97

Isoelectric Point (pI)

31.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 5 - 392 1.8e-66 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 372
AccB7I CCANNNNNTGG 2 cut(s) 521, 686
AccII CGCG 1 cut(s) 876
AciI CCGC 2 cut(s) 411, 599
AclWI GGATC 3 cut(s) 402, 403, 415
AcoI YGGCCR 1 cut(s) 694
AcsI RAATTY 2 cut(s) 917, 1272
AcuI CTGAAG 1 cut(s) 743
AfaI GTAC 3 cut(s) 941, 1262, 1379
AfeI AGCGCT 1 cut(s) 1189
AfiI CCNNNNNNNGG 5 cut(s) 195, 336, 521, 686, 816
AgsI TTSAA 8 cut(s) 511, 784, 866, 888, 922, 934, 997, 1291
AhlI ACTAGT 1 cut(s) 530
AjnI CCWGG 3 cut(s) 942, 981, 1219
AjuI GAANNNNNNNTTGG 2 cut(s) 1354, 1386
Alw21I GWGCWC 1 cut(s) 263
Alw26I GTCTC 9 cut(s) 250, 450, 452, 530, 722, 724, 752, 1058, 1108
AlwI GGATC 3 cut(s) 402, 403, 415
AlwNI CAGNNNCTG 1 cut(s) 425
Aor51HI AGCGCT 1 cut(s) 1189
AoxI GGCC 4 cut(s) 303, 578, 694, 979
ApeKI GCWGC 2 cut(s) 799, 1055
ApoI RAATTY 2 cut(s) 917, 1272
Asp700I GAANNNNTTC 1 cut(s) 870
AspLEI GCGC 3 cut(s) 93, 113, 1190
AspS9I GGNCC 2 cut(s) 303, 578
AsuHPI GGTGA 3 cut(s) 905, 959, 1183
AxyI CCTNAGG 1 cut(s) 976
BaeI ACNNNNGTAYC 4 cut(s) 612, 645, 1369, 1402
BalI TGGCCA 1 cut(s) 696
BamHI GGATCC 1 cut(s) 407
BanII GRGCYC 1 cut(s) 263
BarI GAAGNNNNNNTAC 2 cut(s) 1375, 1407
Bbv12I GWGCWC 1 cut(s) 263
BbvCI CCTCAGC 1 cut(s) 1059
BbvI GCAGC 2 cut(s) 811, 1067
BccI CCATC 2 cut(s) 479, 1121
BciT130I CCWGG 3 cut(s) 944, 983, 1221
BciVI GTATCC 1 cut(s) 1235
BclI TGATCA 1 cut(s) 1347
BcoDI GTCTC 9 cut(s) 250, 450, 452, 530, 722, 724, 752, 1058, 1108
BcuI ACTAGT 1 cut(s) 530
BfaI CTAG 6 cut(s) 228, 449, 531, 953, 1122, 1404
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 2 cut(s) 114, 1191
BfuI GTATCC 1 cut(s) 1235
BisI GCNGC 2 cut(s) 800, 1056
BlsI GCNGC 2 cut(s) 801, 1057
Bme1390I CCNGG 3 cut(s) 944, 983, 1221
BmgT120I GGNCC 2 cut(s) 303, 578
BmiI GGNNCC 3 cut(s) 217, 409, 1302
BmrFI CCNGG 3 cut(s) 944, 983, 1221
BmrI ACTGGG 3 cut(s) 545, 664, 743
BmsI GCATC 6 cut(s) 46, 223, 592, 763, 1153, 1233
BmuI ACTGGG 3 cut(s) 545, 664, 743
BpmI CTGGAG 1 cut(s) 948
Bpu10I CCTNAGC 2 cut(s) 126, 1059
BpuEI CTTGAG 2 cut(s) 137, 1112
BsaI GGTCTC 3 cut(s) 452, 530, 722
BsaJI CCNNGG 3 cut(s) 171, 943, 1219
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 336, 521, 686, 816
Bse1I ACTGG 7 cut(s) 387, 551, 670, 680, 697, 738, 965
Bse21I CCTNAGG 1 cut(s) 976
Bse3DI GCAATG 2 cut(s) 649, 912
BseBI CCWGG 3 cut(s) 944, 983, 1221
BseDI CCNNGG 3 cut(s) 171, 943, 1219
BseGI GGATG 3 cut(s) 546, 607, 775
BseLI CCNNNNNNNGG 5 cut(s) 195, 336, 521, 686, 816
BseMI GCAATG 2 cut(s) 649, 912
BseMII CTCAG 4 cut(s) 43, 806, 1050, 1388
BseNI ACTGG 7 cut(s) 387, 551, 670, 680, 697, 738, 965
BseRI GAGGAG 3 cut(s) 48, 271, 1076
BseXI GCAGC 2 cut(s) 811, 1067
BseYI CCCAGC 2 cut(s) 194, 236
Bsh1236I CGCG 1 cut(s) 876
BshFI GGCC 4 cut(s) 305, 580, 696, 981
BsiHKAI GWGCWC 1 cut(s) 263
BslI CCNNNNNNNGG 5 cut(s) 195, 336, 521, 686, 816
BsmAI GTCTC 9 cut(s) 250, 450, 452, 530, 722, 724, 752, 1058, 1108
BsmI GAATGC 2 cut(s) 940, 992
BsnI GGCC 4 cut(s) 305, 580, 696, 981
Bso31I GGTCTC 3 cut(s) 452, 530, 722
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 6 cut(s) 395, 407, 762, 924, 1311, 1347
Bsp68I TCGCGA 1 cut(s) 876
BspACI CCGC 2 cut(s) 411, 599
BspANI GGCC 4 cut(s) 305, 580, 696, 981
BspCNI CTCAG 4 cut(s) 42, 807, 1051, 1387
BspFNI CGCG 1 cut(s) 876
BspLI GGNNCC 3 cut(s) 217, 409, 1302
BspPI GGATC 3 cut(s) 402, 403, 415
BspTNI GGTCTC 3 cut(s) 452, 530, 722
BsrDI GCAATG 2 cut(s) 649, 912
BsrI ACTGG 7 cut(s) 387, 551, 670, 680, 697, 738, 965
BssECI CCNNGG 3 cut(s) 171, 943, 1219
BssMI GATC 6 cut(s) 395, 407, 762, 924, 1311, 1347
BssT1I CCWWGG 1 cut(s) 171
Bst2UI CCWGG 3 cut(s) 944, 983, 1221
Bst4CI ACNGT 4 cut(s) 520, 595, 1366, 1399
BstC8I GCNNGC 3 cut(s) 69, 1236, 1338
BstDEI CTNAG 9 cut(s) 29, 40, 126, 815, 976, 1048, 1059, 1159, 1374
BstEII GGTNACC 1 cut(s) 947
BstF5I GGATG 3 cut(s) 546, 607, 775
BstFNI CGCG 1 cut(s) 876
BstH2I RGCGCY 2 cut(s) 114, 1191
BstHHI GCGC 3 cut(s) 93, 113, 1190
BstKTI GATC 6 cut(s) 398, 410, 765, 927, 1314, 1350
BstMAI GTCTC 9 cut(s) 250, 450, 452, 530, 722, 724, 752, 1058, 1108
BstMBI GATC 6 cut(s) 395, 407, 762, 924, 1311, 1347
BstMWI GCNNNNNNNGC 5 cut(s) 77, 639, 911, 935, 1187
BstNI CCWGG 3 cut(s) 944, 983, 1221
BstPI GGTNACC 1 cut(s) 947
BstSCI CCNGG 3 cut(s) 942, 981, 1219
BstSFI CTRYAG 1 cut(s) 594
BstUI CGCG 1 cut(s) 876
BstV1I GCAGC 2 cut(s) 811, 1067
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
Bsu36I CCTNAGG 1 cut(s) 976
BsuI GTATCC 1 cut(s) 1235
BsuRI GGCC 4 cut(s) 305, 580, 696, 981
BtsCI GGATG 3 cut(s) 546, 607, 775
BtsI GCAGTG 3 cut(s) 100, 119, 189
BtsIMutI CAGTG 9 cut(s) 100, 119, 189, 380, 447, 687, 812, 972, 1251
BtuMI TCGCGA 1 cut(s) 876
Cac8I GCNNGC 3 cut(s) 69, 1236, 1338
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 3 cut(s) 93, 113, 1190
Cfr13I GGNCC 2 cut(s) 303, 578
Csp6I GTAC 3 cut(s) 940, 1261, 1378
CviAII CATG 4 cut(s) 134, 611, 751, 1247
CviQI GTAC 3 cut(s) 940, 1261, 1378
DdeI CTNAG 9 cut(s) 29, 40, 126, 815, 976, 1048, 1059, 1159, 1374
DpnI GATC 6 cut(s) 397, 409, 764, 926, 1313, 1349
DpnII GATC 6 cut(s) 395, 407, 762, 924, 1311, 1347
EaeI YGGCCR 1 cut(s) 694
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 263
Eco31I GGTCTC 3 cut(s) 452, 530, 722
Eco47III AGCGCT 1 cut(s) 1189
Eco53kI GAGCTC 1 cut(s) 261
Eco57I CTGAAG 1 cut(s) 743
Eco81I CCTNAGG 1 cut(s) 976
Eco91I GGTNACC 1 cut(s) 947
EcoICRI GAGCTC 1 cut(s) 261
EcoO65I GGTNACC 1 cut(s) 947
EcoRI GAATTC 1 cut(s) 917
EcoRII CCWGG 3 cut(s) 942, 981, 1219
EcoT14I CCWWGG 1 cut(s) 171
EcoT22I ATGCAT 1 cut(s) 1168
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 4 cut(s) 137, 614, 754, 1250
FalI AAGNNNNNCTT 2 cut(s) 914, 946
FatI CATG 4 cut(s) 133, 610, 750, 1246
FbaI TGATCA 1 cut(s) 1347
Fnu4HI GCNGC 2 cut(s) 800, 1056
FokI GGATG 3 cut(s) 533, 614, 782
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 800, 1056
FspBI CTAG 6 cut(s) 228, 449, 531, 953, 1122, 1404
GlaI GCGC 3 cut(s) 92, 112, 1189
GluI GCNGC 2 cut(s) 800, 1056
GsaI CCCAGC 2 cut(s) 198, 240
GsuI CTGGAG 1 cut(s) 948
HaeII RGCGCY 2 cut(s) 114, 1191
HaeIII GGCC 4 cut(s) 305, 580, 696, 981
HhaI GCGC 3 cut(s) 93, 113, 1190
Hin1II CATG 4 cut(s) 137, 614, 754, 1250
Hin6I GCGC 3 cut(s) 91, 111, 1188
HinP1I GCGC 3 cut(s) 91, 111, 1188
HindIII AAGCTT 2 cut(s) 128, 179
HinfI GANTC 8 cut(s) 36, 104, 139, 364, 543, 784, 844, 1370
HphI GGTGA 3 cut(s) 905, 959, 1183
Hpy188I TCNGA 4 cut(s) 32, 314, 762, 1300
Hpy188III TCNNGA 9 cut(s) 23, 781, 848, 875, 922, 1016, 1122, 1315, 1351
HpyAV CCTTC 2 cut(s) 277, 1285
HpyCH4III ACNGT 4 cut(s) 520, 595, 1366, 1399
HpyCH4IV ACGT 2 cut(s) 290, 1416
HpyCH4V TGCA 4 cut(s) 67, 428, 830, 1166
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 639, 911, 935, 1187
HpyF3I CTNAG 9 cut(s) 29, 40, 126, 815, 976, 1048, 1059, 1159, 1374
HpySE526I ACGT 2 cut(s) 290, 1416
Hsp92II CATG 4 cut(s) 137, 614, 754, 1250
HspAI GCGC 3 cut(s) 91, 111, 1188
Ksp22I TGATCA 1 cut(s) 1347
Kzo9I GATC 6 cut(s) 395, 407, 762, 924, 1311, 1347
LmnI GCTCC 5 cut(s) 258, 473, 1079, 1300, 1415
Lsp1109I GCAGC 2 cut(s) 811, 1067
LweI GCATC 6 cut(s) 46, 223, 592, 763, 1153, 1233
MaeI CTAG 6 cut(s) 228, 449, 531, 953, 1122, 1404
MaeII ACGT 2 cut(s) 290, 1416
MaeIII GTNAC 3 cut(s) 947, 1171, 1399
MalI GATC 6 cut(s) 397, 409, 764, 926, 1313, 1349
MboI GATC 6 cut(s) 395, 407, 762, 924, 1311, 1347
MboII GAAGA 4 cut(s) 17, 749, 878, 1185
MfeI CAATTG 2 cut(s) 573, 1206
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 263
MlsI TGGCCA 1 cut(s) 696
MluCI AATT 9 cut(s) 373, 573, 582, 706, 831, 917, 1006, 1206, 1272
MluNI TGGCCA 1 cut(s) 696
MlyI GAGTC 2 cut(s) 45, 552
MmeI TCCRAC 2 cut(s) 317, 1385
MnlI CCTC 9 cut(s) 26, 38, 249, 341, 810, 1054, 1087, 1211, 1368
Mox20I TGGCCA 1 cut(s) 696
Mph1103I ATGCAT 1 cut(s) 1168
MroXI GAANNNNTTC 1 cut(s) 870
MscI TGGCCA 1 cut(s) 696
MseI TTAA 2 cut(s) 183, 492
MslI CAYNNNNRTG 1 cut(s) 1229
Msp20I TGGCCA 1 cut(s) 696
MspA1I CMGCKG 1 cut(s) 1058
MspR9I CCNGG 3 cut(s) 944, 983, 1221
MunI CAATTG 2 cut(s) 573, 1206
Mva1269I GAATGC 2 cut(s) 940, 992
MvaI CCWGG 3 cut(s) 944, 983, 1221
MvnI CGCG 1 cut(s) 876
MwoI GCNNNNNNNGC 5 cut(s) 77, 639, 911, 935, 1187
NdeII GATC 6 cut(s) 395, 407, 762, 924, 1311, 1347
NlaIII CATG 4 cut(s) 137, 614, 754, 1250
NlaIV GGNNCC 3 cut(s) 217, 409, 1302
NmuCI GTSAC 3 cut(s) 947, 1171, 1399
NruI TCGCGA 1 cut(s) 876
NsiI ATGCAT 1 cut(s) 1168
PctI GAATGC 2 cut(s) 940, 992
PdmI GAANNNNTTC 1 cut(s) 870
PfeI GAWTC 6 cut(s) 104, 139, 364, 784, 844, 1370
PflFI GACNNNGTC 1 cut(s) 953
PflMI CCANNNNNTGG 2 cut(s) 521, 686
PkrI GCNGC 2 cut(s) 801, 1057
PleI GAGTC 2 cut(s) 44, 551
PpsI GAGTC 2 cut(s) 44, 551
PsiI TTATAA 1 cut(s) 372
Psp124BI GAGCTC 1 cut(s) 263
Psp6I CCWGG 3 cut(s) 942, 981, 1219
PspEI GGTNACC 1 cut(s) 947
PspFI CCCAGC 2 cut(s) 194, 236
PspGI CCWGG 3 cut(s) 942, 981, 1219
PspN4I GGNNCC 3 cut(s) 217, 409, 1302
PspPI GGNCC 2 cut(s) 303, 578
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 407
PsyI GACNNNGTC 1 cut(s) 953
PvuII CAGCTG 1 cut(s) 1058
RruI TCGCGA 1 cut(s) 876
RsaI GTAC 3 cut(s) 941, 1262, 1379
RsaNI GTAC 3 cut(s) 940, 1261, 1378
RseI CAYNNNNRTG 1 cut(s) 1229
SacI GAGCTC 1 cut(s) 263
SaqAI TTAA 2 cut(s) 183, 492
SatI GCNGC 2 cut(s) 800, 1056
Sau3AI GATC 6 cut(s) 395, 407, 762, 924, 1311, 1347
Sau96I GGNCC 2 cut(s) 303, 578
SchI GAGTC 2 cut(s) 45, 552
ScrFI CCNGG 3 cut(s) 944, 983, 1221
SduI GDGCHC 1 cut(s) 263
SfaNI GCATC 6 cut(s) 46, 223, 592, 763, 1153, 1233
SfcI CTRYAG 1 cut(s) 594
SmiMI CAYNNNNRTG 1 cut(s) 1229
SmlI CTYRAG 2 cut(s) 152, 1091
SmoI CTYRAG 2 cut(s) 152, 1091
SpeI ACTAGT 1 cut(s) 530
Sse9I AATT 9 cut(s) 373, 573, 582, 706, 831, 917, 1006, 1206, 1272
SsiI CCGC 2 cut(s) 411, 599
SspMI CTAG 6 cut(s) 228, 449, 531, 953, 1122, 1404
SstI GAGCTC 1 cut(s) 263
StyD4I CCNGG 3 cut(s) 942, 981, 1219
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 4 cut(s) 520, 595, 1366, 1399
TaiI ACGT 2 cut(s) 293, 1419
TaqI TCGA 2 cut(s) 535, 1314
TaqII GACCGA 1 cut(s) 476
TasI AATT 9 cut(s) 373, 573, 582, 706, 831, 917, 1006, 1206, 1272
TfiI GAWTC 6 cut(s) 104, 139, 364, 784, 844, 1370
Tru1I TTAA 2 cut(s) 183, 492
Tru9I TTAA 2 cut(s) 183, 492
TscAI CASTG 9 cut(s) 100, 126, 196, 387, 447, 687, 819, 972, 1258
TseFI GTSAC 3 cut(s) 947, 1171, 1399
TseI GCWGC 2 cut(s) 799, 1055
Tsp45I GTSAC 3 cut(s) 947, 1171, 1399
TspDTI ATGAA 5 cut(s) 117, 122, 366, 599, 675
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 9 cut(s) 100, 126, 196, 387, 447, 687, 819, 972, 1258
Tth111I GACNNNGTC 1 cut(s) 953
Van91I CCANNNNNTGG 2 cut(s) 521, 686
XapI RAATTY 2 cut(s) 917, 1272
XbaI TCTAGA 1 cut(s) 1121
XmnI GAANNNNTTC 1 cut(s) 870
XspI CTAG 6 cut(s) 228, 449, 531, 953, 1122, 1404
Zsp2I ATGCAT 1 cut(s) 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.