RchiOBHm_Chr6g0244671

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000427
Physical Location & Seq
Reverse (-)
687631 .. 689693
2063 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21930

Sequence Viewer

Length: 690 bp
ATGGCACTCCATTACTGCCATTATCATCCATGGCTAACTATCTTCTCCTCTCAAACACTCACAGCCAAAAGGCCCAAAATCACAATTGAAGAAGCTACTGTTGATGATCTCCAGCTTGCTTTCAAGCTCAACACTCTGACCTCAAGGCAACTCGTTCTGTTCTACCTGGTTCAAAAACTGAACAAAAACCTTAGGGCTGTGATAGAGGTGAACCCTGATGCTTTATCCCAAGCTGATATAGCTGACAAACAACGCAGAGAATGCAACCACACACTGCCTAAGCTTCATGGCATTCCTATTCTGCTCAAGGATACCATACCAACCAAGGACAAACTCAATACCACAGCTGGATCGTTTGCATTGTTGGGGTCCATTGTTCCTCGAGATGCAGCTGTTGTTTCTAAGCTAAGGAGGTCTGGAGCTATCATTTTAGGGAAGGCTAGCTTGAGTGAATGGTGCCATTTCAGGTCATTCCTAGCACCTTCTGGATGGAGCGCCAGAGGTTCCCAAGGGATGAATCCTTACAATTCATCGGTGGAAGTTTGTGGATCAAGTAGTGGACCTGCAATATCAGTAGCTGCTAATATGGTTTCTGTGACATTAGGGACGGAAACTGACAGCTCCATTATCTGTCCGGCTAGCTTTAACTCTGTGGTGGGCATCAAACCCACTGTTGGTCTCACTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

229

Amino Acids

24.73

Weight (kDa)

9.28

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 58 - 229 1.9e-45 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 571
AccB1I GGYRCC 1 cut(s) 456
AclWI GGATC 2 cut(s) 358, 556
AfiI CCNNNNNNNGG 1 cut(s) 674
AgsI TTSAA 3 cut(s) 89, 124, 173
AhlI ACTAGT 1 cut(s) 683
AjnI CCWGG 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 2 cut(s) 358, 556
AlwNI CAGNNNCTG 1 cut(s) 578
Ama87I CYCGRG 1 cut(s) 381
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 389, 578
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 3 cut(s) 72, 369, 560
AsuHPI GGTGA 1 cut(s) 220
AsuNHI GCTAGC 2 cut(s) 440, 638
AvaI CYCGRG 1 cut(s) 381
AvaII GGWCC 2 cut(s) 369, 560
AxyI CCTNAGG 1 cut(s) 191
BanI GGYRCC 1 cut(s) 456
BbvI GCAGC 2 cut(s) 401, 565
BccI CCATC 1 cut(s) 483
BciT130I CCWGG 1 cut(s) 167
BciVI GTATCC 1 cut(s) 304
BcoDI GTCTC 1 cut(s) 683
BcuI ACTAGT 1 cut(s) 683
BfaI CTAG 4 cut(s) 441, 476, 639, 684
BfoI RGCGCY 1 cut(s) 498
BfuAI ACCTGC 1 cut(s) 571
BfuI GTATCC 1 cut(s) 304
BisI GCNGC 2 cut(s) 390, 579
BlsI GCNGC 2 cut(s) 391, 580
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 2 cut(s) 369, 560
BmeT110I CYCGRG 1 cut(s) 381
BmgT120I GGNCC 3 cut(s) 72, 369, 560
BmiI GGNNCC 3 cut(s) 370, 458, 505
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 3 cut(s) 208, 376, 669
BmtI GCTAGC 2 cut(s) 444, 642
BpmI CTGGAG 2 cut(s) 95, 438
Bpu10I CCTNAGC 2 cut(s) 279, 407
BpuEI CTTGAG 3 cut(s) 127, 290, 466
BsaI GGTCTC 1 cut(s) 683
BsaJI CCNNGG 3 cut(s) 29, 324, 508
Bsc4I CCNNNNNNNGG 1 cut(s) 674
Bse21I CCTNAGG 1 cut(s) 191
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 3 cut(s) 29, 324, 508
BseGI GGATG 3 cut(s) 25, 494, 519
BseLI CCNNNNNNNGG 1 cut(s) 674
BseRI GAGGAG 1 cut(s) 37
BseXI GCAGC 2 cut(s) 401, 565
BshFI GGCC 1 cut(s) 73
BshNI GGYRCC 1 cut(s) 456
BsiHKCI CYCGRG 1 cut(s) 381
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 1 cut(s) 683
BsmFI GGGAC 1 cut(s) 619
BsmI GAATGC 2 cut(s) 266, 291
BsnI GGCC 1 cut(s) 73
Bso31I GGTCTC 1 cut(s) 683
BsoBI CYCGRG 1 cut(s) 381
Bsp143I GATC 3 cut(s) 106, 350, 548
Bsp19I CCATGG 1 cut(s) 29
BspANI GGCC 1 cut(s) 73
BspLI GGNNCC 3 cut(s) 370, 458, 505
BspMI ACCTGC 1 cut(s) 571
BspOI GCTAGC 2 cut(s) 444, 642
BspPI GGATC 2 cut(s) 358, 556
BspT107I GGYRCC 1 cut(s) 456
BspTNI GGTCTC 1 cut(s) 683
BssECI CCNNGG 3 cut(s) 29, 324, 508
BssMI GATC 3 cut(s) 106, 350, 548
BssT1I CCWWGG 3 cut(s) 29, 324, 508
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 100, 673
BstAPI GCANNNNNTGC 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 117, 442, 640
BstDEI CTNAG 4 cut(s) 191, 279, 402, 407
BstDSI CCRYGG 1 cut(s) 29
BstF5I GGATG 3 cut(s) 25, 494, 519
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 3 cut(s) 109, 353, 551
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 3 cut(s) 106, 350, 548
BstMWI GCNNNNNNNGC 2 cut(s) 239, 261
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstV1I GCAGC 2 cut(s) 401, 565
Bsu36I CCTNAGG 1 cut(s) 191
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 1 cut(s) 73
BtgI CCRYGG 1 cut(s) 29
BtsCI GGATG 3 cut(s) 25, 494, 519
BtsI GCAGTG 1 cut(s) 272
BtsIMutI CAGTG 2 cut(s) 272, 669
BveI ACCTGC 1 cut(s) 571
Cac8I GCNNGC 3 cut(s) 117, 442, 640
CaiI CAGNNNCTG 1 cut(s) 578
CfoI GCGC 1 cut(s) 497
Cfr13I GGNCC 3 cut(s) 72, 369, 560
CsiI ACCWGGT 1 cut(s) 165
CviAII CATG 2 cut(s) 30, 287
DdeI CTNAG 4 cut(s) 191, 279, 402, 407
DpnI GATC 3 cut(s) 108, 352, 550
DpnII GATC 3 cut(s) 106, 350, 548
Eco130I CCWWGG 3 cut(s) 29, 324, 508
Eco31I GGTCTC 1 cut(s) 683
Eco47I GGWCC 2 cut(s) 369, 560
Eco81I CCTNAGG 1 cut(s) 191
Eco88I CYCGRG 1 cut(s) 381
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 3 cut(s) 29, 324, 508
ErhI CCWWGG 3 cut(s) 29, 324, 508
FaeI CATG 2 cut(s) 33, 290
FaiI YATR 5 cut(s) 31, 239, 288, 317, 587
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FaqI GGGAC 1 cut(s) 619
FatI CATG 2 cut(s) 29, 286
Fnu4HI GCNGC 2 cut(s) 390, 579
FokI GGATG 3 cut(s) 12, 501, 526
Fsp4HI GCNGC 2 cut(s) 390, 579
FspBI CTAG 4 cut(s) 441, 476, 639, 684
GlaI GCGC 1 cut(s) 496
GluI GCNGC 2 cut(s) 390, 579
GsuI CTGGAG 2 cut(s) 95, 438
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 1 cut(s) 73
HapII CCGG 1 cut(s) 635
HhaI GCGC 1 cut(s) 497
Hin1II CATG 2 cut(s) 33, 290
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 1 cut(s) 517
HpaII CCGG 1 cut(s) 635
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 2 cut(s) 211, 560
Hpy188I TCNGA 1 cut(s) 138
Hpy188III TCNNGA 3 cut(s) 383, 417, 486
Hpy8I GTNNAC 2 cut(s) 211, 560
HpyAV CCTTC 2 cut(s) 430, 492
HpyCH4III ACNGT 2 cut(s) 100, 673
HpyCH4V TGCA 4 cut(s) 264, 359, 389, 566
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 261
HpyF3I CTNAG 4 cut(s) 191, 279, 402, 407
Hsp92II CATG 2 cut(s) 33, 290
HspAI GCGC 1 cut(s) 495
Kzo9I GATC 3 cut(s) 106, 350, 548
LmnI GCTCC 3 cut(s) 419, 492, 626
Lsp1109I GCAGC 2 cut(s) 401, 565
LweI GCATC 3 cut(s) 208, 376, 669
MabI ACCWGGT 1 cut(s) 165
MaeI CTAG 4 cut(s) 441, 476, 639, 684
MaeIII GTNAC 1 cut(s) 595
MalI GATC 3 cut(s) 108, 352, 550
MboI GATC 3 cut(s) 106, 350, 548
MboII GAAGA 2 cut(s) 34, 101
MfeI CAATTG 1 cut(s) 84
MluCI AATT 2 cut(s) 84, 526
MnlI CCTC 6 cut(s) 58, 151, 199, 390, 405, 494
MseI TTAA 1 cut(s) 645
MspA1I CMGCKG 2 cut(s) 347, 392
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 1 cut(s) 167
MunI CAATTG 1 cut(s) 84
Mva1269I GAATGC 2 cut(s) 266, 291
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 2 cut(s) 239, 261
NcoI CCATGG 1 cut(s) 29
NdeII GATC 3 cut(s) 106, 350, 548
NheI GCTAGC 2 cut(s) 440, 638
NlaIII CATG 2 cut(s) 33, 290
NlaIV GGNNCC 3 cut(s) 370, 458, 505
NmuCI GTSAC 1 cut(s) 595
PaeR7I CTCGAG 1 cut(s) 381
PctI GAATGC 2 cut(s) 266, 291
PfeI GAWTC 1 cut(s) 517
PkrI GCNGC 2 cut(s) 391, 580
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 3 cut(s) 370, 458, 505
PspPI GGNCC 3 cut(s) 72, 369, 560
PstNI CAGNNNCTG 1 cut(s) 578
PvuII CAGCTG 2 cut(s) 347, 392
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 2 cut(s) 390, 579
Sau3AI GATC 3 cut(s) 106, 350, 548
Sau96I GGNCC 3 cut(s) 72, 369, 560
ScrFI CCNGG 1 cut(s) 167
SexAI ACCWGGT 1 cut(s) 165
SfaNI GCATC 3 cut(s) 208, 376, 669
Sfr274I CTCGAG 1 cut(s) 381
SinI GGWCC 2 cut(s) 369, 560
SlaI CTCGAG 1 cut(s) 381
SmlI CTYRAG 4 cut(s) 142, 305, 381, 445
SmoI CTYRAG 4 cut(s) 142, 305, 381, 445
SpeI ACTAGT 1 cut(s) 683
Sse9I AATT 2 cut(s) 84, 526
SspMI CTAG 4 cut(s) 441, 476, 639, 684
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 3 cut(s) 29, 324, 508
TaaI ACNGT 2 cut(s) 100, 673
TaqI TCGA 1 cut(s) 382
TasI AATT 2 cut(s) 84, 526
TfiI GAWTC 1 cut(s) 517
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 2 cut(s) 279, 676
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 2 cut(s) 389, 578
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 3 cut(s) 275, 519, 530
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 2 cut(s) 279, 676
VpaK11BI GGWCC 2 cut(s) 369, 560
XhoI CTCGAG 1 cut(s) 381
XspI CTAG 4 cut(s) 441, 476, 639, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.