RLG00000015339

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000427
Physical Location & Seq
Forward (+)
64769756 .. 64772198
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015339

Sequence Viewer

Length: 1110 bp
ATGAACAGAATCAAAGACATAAGCTTTTCTTATGATGTGCAGAATCCTTATAATTCATCACTGGAAGTTTGTGGATCAAGTAGTGGATCCGCAATATCAGTATCTGCAAATATGGTATCAGTGTCTCTAGGAACAGAGACAGATAGCTCCATCTTATGTCCAGCGAGTTTTAACTCGGTAGTGGGCTTCAAACCAACGGTTGGTCTCACTAGTCGAGCAGGAGTCATCCCAGTCAGTCCAAGACAGGACACAATTGGGCCAATTTGTAGGACTGTAGCGGATGCTGTTCATGTCCTTGATACCATTGTTGGCATTGATAGCAATGACAAGGCAACAAATGAAGTATCCCAGTATATTCCAGTGGGTGGCTATGGCCAGTTTCTCAATTCTAATGGTCTCAAAGGAAAGAGACTGGGAATAGTCAGTCTCATGTTTCTTCAGATCAAGGATGATGTTTTCTTGAATCAAACTTTTGAGCAGCATTTTACCACACTGAGGGAACAAGGTGCAATTTTGGTTGAGAATCTGGAAATAGCCAACCTTGAAGATATTCGTGATTTTACTTCTAGTGGTGAGTTTGTAGCAATGTTAGCTGAATTCAAGATCGCCTTGAATGCGTACCTGGGTGATCTAGTCAAATCTCCAGTGAGAACCTTAGGCCAGGTTATAGCATTCAACAAGAATAATTCTTATCTGGAAAAGAATGATGATTATGGACAAAATCTCTTAGAAGCAGCTGAGGAGACTAATGGAATGGGAGCAAAAGAGCTTGAGGCATTGTCAAATATAGAGAGACTATCTAGAGATGGATTTGAGAAGTTGATGACAGATAATAACTTAGATGCATTGGTGACTTATGCTAATAGCGCTTCTTCTATCCTCGCAATTGGTGGTTTCCCAGGCATCGTTGTGCCAGCAGGATATCACGATACTGATAAGTACCCTTTTGGAATTTGCTTTGGGGGATTGAAGGGTTCGGAGCCAAAGCTGATCGAGATTGCCTATGCCTTTGAGCAAGCAACTATGATCAGGAAGTCTCCAACTGTTGATTCTCAGTACCAAAGTATCTCTACCACCGTCACTAGAAGCTCCAACGTAGCTACTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

370

Amino Acids

39.77

Weight (kDa)

4.72

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 12 - 285 3.5e-39 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 51
AccB7I CCANNNNNTGG 2 cut(s) 200, 365
AciI CCGC 2 cut(s) 90, 278
AclWI GGATC 3 cut(s) 81, 82, 94
AcoI YGGCCR 1 cut(s) 373
AcsI RAATTY 2 cut(s) 596, 951
AcuI CTGAAG 1 cut(s) 422
AfaI GTAC 3 cut(s) 620, 941, 1058
AfeI AGCGCT 1 cut(s) 868
AfiI CCNNNNNNNGG 3 cut(s) 200, 365, 495
AgsI TTSAA 7 cut(s) 190, 463, 545, 601, 613, 676, 970
AhlI ACTAGT 1 cut(s) 209
AjnI CCWGG 3 cut(s) 621, 660, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 1033, 1065
AluBI AGCT 8 cut(s) 24, 147, 593, 737, 769, 988, 1089, 1100
AluI AGCT 8 cut(s) 24, 147, 593, 737, 769, 988, 1089, 1100
Alw26I GTCTC 9 cut(s) 129, 131, 209, 401, 403, 431, 737, 787, 1041
AlwI GGATC 3 cut(s) 81, 82, 94
AlwNI CAGNNNCTG 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 868
AoxI GGCC 3 cut(s) 257, 373, 658
ApeKI GCWGC 2 cut(s) 478, 734
ApoI RAATTY 2 cut(s) 596, 951
Asp700I GAANNNNTTC 1 cut(s) 549
AspLEI GCGC 1 cut(s) 869
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 3 cut(s) 584, 638, 862
AxyI CCTNAGG 1 cut(s) 655
BaeI ACNNNNGTAYC 4 cut(s) 291, 324, 1048, 1081
BalI TGGCCA 1 cut(s) 375
BamHI GGATCC 1 cut(s) 86
BbvCI CCTCAGC 1 cut(s) 738
BbvI GCAGC 2 cut(s) 490, 746
BccI CCATC 2 cut(s) 158, 800
BciT130I CCWGG 3 cut(s) 623, 662, 900
BciVI GTATCC 1 cut(s) 355
BclI TGATCA 1 cut(s) 1026
BcoDI GTCTC 9 cut(s) 129, 131, 209, 401, 403, 431, 737, 787, 1041
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 6 cut(s) 128, 210, 567, 632, 801, 1083
BfmI CTRYAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 870
BfuI GTATCC 1 cut(s) 355
BisI GCNGC 2 cut(s) 479, 735
BlsI GCNGC 2 cut(s) 480, 736
Bme1390I CCNGG 3 cut(s) 623, 662, 900
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 2 cut(s) 88, 981
BmrFI CCNGG 3 cut(s) 623, 662, 900
BmrI ACTGGG 3 cut(s) 224, 343, 422
BmsI GCATC 3 cut(s) 271, 832, 912
BmuI ACTGGG 3 cut(s) 224, 343, 422
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 1 cut(s) 738
BpuEI CTTGAG 1 cut(s) 791
BsaI GGTCTC 2 cut(s) 209, 401
BsaJI CCNNGG 2 cut(s) 622, 898
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 365, 495
Bse1I ACTGG 7 cut(s) 66, 230, 349, 359, 376, 417, 644
Bse21I CCTNAGG 1 cut(s) 655
Bse3DI GCAATG 2 cut(s) 328, 591
BseBI CCWGG 3 cut(s) 623, 662, 900
BseDI CCNNGG 2 cut(s) 622, 898
BseGI GGATG 3 cut(s) 225, 286, 454
BseLI CCNNNNNNNGG 3 cut(s) 200, 365, 495
BseMI GCAATG 2 cut(s) 328, 591
BseMII CTCAG 3 cut(s) 485, 729, 1067
BseNI ACTGG 7 cut(s) 66, 230, 349, 359, 376, 417, 644
BseRI GAGGAG 1 cut(s) 755
BseXI GCAGC 2 cut(s) 490, 746
BsgI GTGCAG 1 cut(s) 59
BshFI GGCC 3 cut(s) 259, 375, 660
BslI CCNNNNNNNGG 3 cut(s) 200, 365, 495
BsmAI GTCTC 9 cut(s) 129, 131, 209, 401, 403, 431, 737, 787, 1041
BsmI GAATGC 2 cut(s) 619, 671
BsnI GGCC 3 cut(s) 259, 375, 660
Bso31I GGTCTC 2 cut(s) 209, 401
Bsp143I GATC 7 cut(s) 74, 86, 441, 603, 628, 990, 1026
BspACI CCGC 2 cut(s) 90, 278
BspANI GGCC 3 cut(s) 259, 375, 660
BspCNI CTCAG 3 cut(s) 486, 730, 1066
BspLI GGNNCC 2 cut(s) 88, 981
BspPI GGATC 3 cut(s) 81, 82, 94
BspTNI GGTCTC 2 cut(s) 209, 401
BsrDI GCAATG 2 cut(s) 328, 591
BsrI ACTGG 7 cut(s) 66, 230, 349, 359, 376, 417, 644
BssECI CCNNGG 2 cut(s) 622, 898
BssMI GATC 7 cut(s) 74, 86, 441, 603, 628, 990, 1026
Bst2UI CCWGG 3 cut(s) 623, 662, 900
Bst4CI ACNGT 4 cut(s) 199, 274, 1045, 1078
BstC8I GCNNGC 2 cut(s) 915, 1017
BstDEI CTNAG 6 cut(s) 494, 655, 727, 738, 838, 1053
BstF5I GGATG 3 cut(s) 225, 286, 454
BstH2I RGCGCY 1 cut(s) 870
BstHHI GCGC 1 cut(s) 869
BstKTI GATC 7 cut(s) 77, 89, 444, 606, 631, 993, 1029
BstMAI GTCTC 9 cut(s) 129, 131, 209, 401, 403, 431, 737, 787, 1041
BstMBI GATC 7 cut(s) 74, 86, 441, 603, 628, 990, 1026
BstMWI GCNNNNNNNGC 4 cut(s) 318, 590, 614, 866
BstNI CCWGG 3 cut(s) 623, 662, 900
BstSCI CCNGG 3 cut(s) 621, 660, 898
BstSFI CTRYAG 1 cut(s) 273
BstV1I GCAGC 2 cut(s) 490, 746
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
Bsu36I CCTNAGG 1 cut(s) 655
BsuI GTATCC 1 cut(s) 355
BsuRI GGCC 3 cut(s) 259, 375, 660
BtsCI GGATG 3 cut(s) 225, 286, 454
BtsIMutI CAGTG 5 cut(s) 59, 126, 366, 491, 651
Cac8I GCNNGC 2 cut(s) 915, 1017
CaiI CAGNNNCTG 1 cut(s) 104
CfoI GCGC 1 cut(s) 869
Cfr13I GGNCC 1 cut(s) 257
Csp6I GTAC 3 cut(s) 619, 940, 1057
CviAII CATG 2 cut(s) 290, 430
CviQI GTAC 3 cut(s) 619, 940, 1057
DdeI CTNAG 6 cut(s) 494, 655, 727, 738, 838, 1053
DpnI GATC 7 cut(s) 76, 88, 443, 605, 630, 992, 1028
DpnII GATC 7 cut(s) 74, 86, 441, 603, 628, 990, 1026
EaeI YGGCCR 1 cut(s) 373
Eco31I GGTCTC 2 cut(s) 209, 401
Eco32I GATATC 1 cut(s) 923
Eco47III AGCGCT 1 cut(s) 868
Eco57I CTGAAG 1 cut(s) 422
Eco81I CCTNAGG 1 cut(s) 655
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 3 cut(s) 621, 660, 898
EcoRV GATATC 1 cut(s) 923
EcoT22I ATGCAT 1 cut(s) 847
FaeI CATG 2 cut(s) 293, 433
FalI AAGNNNNNCTT 4 cut(s) 13, 45, 593, 625
FatI CATG 2 cut(s) 289, 429
FbaI TGATCA 1 cut(s) 1026
Fnu4HI GCNGC 2 cut(s) 479, 735
FokI GGATG 3 cut(s) 212, 293, 461
Fsp4HI GCNGC 2 cut(s) 479, 735
FspBI CTAG 6 cut(s) 128, 210, 567, 632, 801, 1083
GlaI GCGC 1 cut(s) 868
GluI GCNGC 2 cut(s) 479, 735
GsuI CTGGAG 1 cut(s) 627
HaeII RGCGCY 1 cut(s) 870
HaeIII GGCC 3 cut(s) 259, 375, 660
HhaI GCGC 1 cut(s) 869
Hin1II CATG 2 cut(s) 293, 433
Hin6I GCGC 1 cut(s) 867
HinP1I GCGC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 22
HinfI GANTC 6 cut(s) 9, 43, 222, 463, 523, 1049
HphI GGTGA 3 cut(s) 584, 638, 862
Hpy188I TCNGA 2 cut(s) 441, 979
Hpy188III TCNNGA 9 cut(s) 460, 527, 554, 601, 695, 801, 926, 994, 1030
HpyAV CCTTC 1 cut(s) 964
HpyCH4III ACNGT 4 cut(s) 199, 274, 1045, 1078
HpyCH4IV ACGT 1 cut(s) 1095
HpyCH4V TGCA 4 cut(s) 40, 107, 509, 845
HpyF10VI GCNNNNNNNGC 4 cut(s) 318, 590, 614, 866
HpyF3I CTNAG 6 cut(s) 494, 655, 727, 738, 838, 1053
HpySE526I ACGT 1 cut(s) 1095
Hsp92II CATG 2 cut(s) 293, 433
HspAI GCGC 1 cut(s) 867
Ksp22I TGATCA 1 cut(s) 1026
Kzo9I GATC 7 cut(s) 74, 86, 441, 603, 628, 990, 1026
LmnI GCTCC 4 cut(s) 152, 758, 979, 1094
Lsp1109I GCAGC 2 cut(s) 490, 746
LweI GCATC 3 cut(s) 271, 832, 912
MaeI CTAG 6 cut(s) 128, 210, 567, 632, 801, 1083
MaeII ACGT 1 cut(s) 1095
MaeIII GTNAC 2 cut(s) 850, 1078
MalI GATC 7 cut(s) 76, 88, 443, 605, 630, 992, 1028
MboI GATC 7 cut(s) 74, 86, 441, 603, 628, 990, 1026
MboII GAAGA 3 cut(s) 428, 557, 864
MfeI CAATTG 2 cut(s) 252, 885
MflI RGATCY 1 cut(s) 86
MlsI TGGCCA 1 cut(s) 375
MluCI AATT 9 cut(s) 52, 252, 261, 385, 510, 596, 685, 885, 951
MluNI TGGCCA 1 cut(s) 375
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 1 cut(s) 1064
MnlI CCTC 4 cut(s) 489, 733, 766, 890
Mox20I TGGCCA 1 cut(s) 375
Mph1103I ATGCAT 1 cut(s) 847
MroXI GAANNNNTTC 1 cut(s) 549
MscI TGGCCA 1 cut(s) 375
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 1 cut(s) 908
Msp20I TGGCCA 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 737
MspR9I CCNGG 3 cut(s) 623, 662, 900
MunI CAATTG 2 cut(s) 252, 885
Mva1269I GAATGC 2 cut(s) 619, 671
MvaI CCWGG 3 cut(s) 623, 662, 900
MwoI GCNNNNNNNGC 4 cut(s) 318, 590, 614, 866
NdeII GATC 7 cut(s) 74, 86, 441, 603, 628, 990, 1026
NlaIII CATG 2 cut(s) 293, 433
NlaIV GGNNCC 2 cut(s) 88, 981
NmuCI GTSAC 2 cut(s) 850, 1078
NsiI ATGCAT 1 cut(s) 847
PctI GAATGC 2 cut(s) 619, 671
PdmI GAANNNNTTC 1 cut(s) 549
PfeI GAWTC 5 cut(s) 9, 43, 463, 523, 1049
PflMI CCANNNNNTGG 2 cut(s) 200, 365
PkrI GCNGC 2 cut(s) 480, 736
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
PsiI TTATAA 1 cut(s) 51
Psp6I CCWGG 3 cut(s) 621, 660, 898
PspGI CCWGG 3 cut(s) 621, 660, 898
PspN4I GGNNCC 2 cut(s) 88, 981
PspPI GGNCC 1 cut(s) 257
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 737
RsaI GTAC 3 cut(s) 620, 941, 1058
RsaNI GTAC 3 cut(s) 619, 940, 1057
RseI CAYNNNNRTG 1 cut(s) 908
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 2 cut(s) 479, 735
Sau3AI GATC 7 cut(s) 74, 86, 441, 603, 628, 990, 1026
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 1 cut(s) 231
ScrFI CCNGG 3 cut(s) 623, 662, 900
SfaNI GCATC 3 cut(s) 271, 832, 912
SfcI CTRYAG 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 908
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SpeI ACTAGT 1 cut(s) 209
Sse9I AATT 9 cut(s) 52, 252, 261, 385, 510, 596, 685, 885, 951
SsiI CCGC 2 cut(s) 90, 278
SspMI CTAG 6 cut(s) 128, 210, 567, 632, 801, 1083
StyD4I CCNGG 3 cut(s) 621, 660, 898
TaaI ACNGT 4 cut(s) 199, 274, 1045, 1078
TaiI ACGT 1 cut(s) 1098
TaqI TCGA 2 cut(s) 214, 993
TasI AATT 9 cut(s) 52, 252, 261, 385, 510, 596, 685, 885, 951
TfiI GAWTC 5 cut(s) 9, 43, 463, 523, 1049
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 5 cut(s) 66, 126, 366, 498, 651
TseFI GTSAC 2 cut(s) 850, 1078
TseI GCWGC 2 cut(s) 478, 734
Tsp45I GTSAC 2 cut(s) 850, 1078
TspDTI ATGAA 4 cut(s) 17, 45, 278, 354
TspRI CASTG 5 cut(s) 66, 126, 366, 498, 651
Van91I CCANNNNNTGG 2 cut(s) 200, 365
XapI RAATTY 2 cut(s) 596, 951
XbaI TCTAGA 1 cut(s) 800
XmnI GAANNNNTTC 1 cut(s) 549
XspI CTAG 6 cut(s) 128, 210, 567, 632, 801, 1083
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.