Rorug05G0510100

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000427
Physical Location & Seq
Reverse (-)
69409533 .. 69411782
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0510100.1

Sequence Viewer

Length: 795 bp
ATGAAAAAAAGATCAAAACGCGGTGTGGAAAAAGATGGGTATATAAGGCAGATAGATTGGGAGCTCAGACCCGGTGGCATGCTTGTCCAAAAACGAGACACTGGGGATGCCTCTTCTGGACCTATGATCAAGATCAAGGTTTCTCATGATTCTTATTACCACGATGTCACTGTACGTTGTCGATCAACTTTTGGCGATCTGAAGAGAATTCTTGCAAATGAGACTGGATTAGAGCCAAAGGCGCAAAGGTTGTTGTATAGAGGAAAAGAGAAGGATGATGTTGAATGTTTGCACATCGCAGGTGTAAAAGATATGTCTAAGATTATGTTATTGGAAGACCCAGCTAGCAAAGAGAAGAAGCTTGAAGAAATGAACAAAAATCAAGGTATACTGAAAGCCTATGAAGAGGTTGCCAAAGTTAGAGCACAAGTTGACAAGCTCTCTCAAAAGATTGAGAATATGGAGACAACACTATGCAATGGTACCAAGGTCGCAGATACGGAGATTGTGGTCTTAACAGAGTTGCTCATGGTGGAGTTGCTCAAATTGGATTCCATTGAGGCAGATGGAGAAGCAAAAGTACAGAGGCGGGTAGAGGTTCGTCGTGTACAAAGTTTCGTTGATACACTCGACAACCTAAAAGCAAGAAACTCTAATCCATTCAGCAAGAGTAGCAACTCTGTCTCAGTGACTACAAATTGGGAGAGTTTTGAATTGGGGGTTGGAAGCCTGCATGCCCCAACCCCATTGAAATCTTCTACCAAGATAACTCAAGATTGGGAATTGTTTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

264

Amino Acids

29.86

Weight (kDa)

8.27

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ubiquitin PF00240 43 - 111 3.6e-07 Ubiquitin family
BAG PF02179 137 - 213 2.7e-15 BAG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 290
Acc36I ACCTGC 1 cut(s) 290
Acc65I GGTACC 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 482
AccI GTMKAC 1 cut(s) 388
AccII CGCG 1 cut(s) 21
AciI CCGC 2 cut(s) 21, 589
AcsI RAATTY 1 cut(s) 207
AcuI CTGAAG 1 cut(s) 221
AfaI GTAC 4 cut(s) 174, 484, 582, 609
AgsI TTSAA 4 cut(s) 284, 365, 713, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 705, 737
AluBI AGCT 4 cut(s) 64, 344, 361, 439
AluI AGCT 4 cut(s) 64, 344, 361, 439
Alw21I GWGCWC 2 cut(s) 66, 427
Alw26I GTCTC 4 cut(s) 90, 215, 458, 688
ApoI RAATTY 1 cut(s) 207
Asp718I GGTACC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 72
AsuNHI GCTAGC 1 cut(s) 344
AvaII GGWCC 1 cut(s) 119
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 1 cut(s) 66
BarI GAAGNNNNNNTAC 2 cut(s) 564, 596
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 2 cut(s) 66, 427
BccI CCATC 2 cut(s) 29, 560
BclI TGATCA 1 cut(s) 126
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 4 cut(s) 90, 215, 458, 688
BfaI CTAG 1 cut(s) 345
BfuAI ACCTGC 1 cut(s) 290
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 484
BmrFI CCNGG 1 cut(s) 72
BmrI ACTGGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 97
BmtI GCTAGC 1 cut(s) 348
BmuI ACTGGG 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 486
Bse1I ACTGG 2 cut(s) 106, 229
Bse3DI GCAATG 1 cut(s) 484
Bse8I GATNNNNATC 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 486
BseGI GGATG 2 cut(s) 112, 280
BseJI GATNNNNATC 1 cut(s) 131
BseMI GCAATG 1 cut(s) 484
BseMII CTCAG 2 cut(s) 79, 699
BseNI ACTGG 2 cut(s) 106, 229
BseYI CCCAGC 1 cut(s) 340
Bsh1236I CGCG 1 cut(s) 21
BshNI GGYRCC 1 cut(s) 482
BsiHKAI GWGCWC 2 cut(s) 66, 427
BsiSI CCGG 1 cut(s) 72
BsmAI GTCTC 4 cut(s) 90, 215, 458, 688
Bsp1286I GDGCHC 2 cut(s) 66, 427
Bsp1407I TGTACA 1 cut(s) 607
Bsp143I GATC 5 cut(s) 11, 126, 132, 182, 196
BspACI CCGC 2 cut(s) 21, 589
BspCNI CTCAG 2 cut(s) 78, 698
BspFNI CGCG 1 cut(s) 21
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 290
BspOI GCTAGC 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 484
BsrGI TGTACA 1 cut(s) 607
BsrI ACTGG 2 cut(s) 106, 229
BssECI CCNNGG 1 cut(s) 486
BssMI GATC 5 cut(s) 11, 126, 132, 182, 196
BssNAI GTATAC 1 cut(s) 389
BssT1I CCWWGG 1 cut(s) 486
Bst1107I GTATAC 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 3 cut(s) 118, 197, 399
BstAUI TGTACA 1 cut(s) 607
BstC8I GCNNGC 4 cut(s) 80, 346, 731, 735
BstDEI CTNAG 3 cut(s) 65, 318, 685
BstF5I GGATG 2 cut(s) 112, 280
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 5 cut(s) 14, 129, 135, 185, 199
BstMAI GTCTC 4 cut(s) 90, 215, 458, 688
BstMBI GATC 5 cut(s) 11, 126, 132, 182, 196
BstMWI GCNNNNNNNGC 2 cut(s) 241, 672
BstNSI RCATGY 2 cut(s) 82, 737
BstSCI CCNGG 1 cut(s) 70
BstUI CGCG 1 cut(s) 21
BstV2I GAAGAC 1 cut(s) 342
BstZ17I GTATAC 1 cut(s) 389
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 2 cut(s) 112, 280
BtsIMutI CAGTG 3 cut(s) 99, 168, 693
BveI ACCTGC 1 cut(s) 290
Cac8I GCNNGC 4 cut(s) 80, 346, 731, 735
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 4 cut(s) 173, 483, 581, 608
CviAII CATG 4 cut(s) 79, 146, 529, 734
CviJI RGCY 7 cut(s) 64, 235, 344, 361, 398, 439, 729
CviKI_1 RGCY 7 cut(s) 64, 235, 344, 361, 398, 439, 729
CviQI GTAC 4 cut(s) 173, 483, 581, 608
DdeI CTNAG 3 cut(s) 65, 318, 685
DpnI GATC 5 cut(s) 13, 128, 134, 184, 198
DpnII GATC 5 cut(s) 11, 126, 132, 182, 196
Eam1104I CTCTTC 3 cut(s) 118, 197, 399
EarI CTCTTC 3 cut(s) 118, 197, 399
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 486
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 486
FaeI CATG 4 cut(s) 82, 149, 532, 737
FatI CATG 4 cut(s) 78, 145, 528, 733
FauI CCCGC 1 cut(s) 582
FbaI TGATCA 1 cut(s) 126
FblI GTMKAC 1 cut(s) 388
FokI GGATG 2 cut(s) 119, 287
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 1 cut(s) 345
GlaI GCGC 1 cut(s) 243
GsaI CCCAGC 1 cut(s) 344
HapII CCGG 1 cut(s) 72
HhaI GCGC 1 cut(s) 244
Hin1II CATG 4 cut(s) 82, 149, 532, 737
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 2 cut(s) 149, 551
HpaII CCGG 1 cut(s) 72
Hpy166II GTNNAC 3 cut(s) 389, 433, 608
Hpy188I TCNGA 2 cut(s) 68, 201
Hpy188III TCNNGA 4 cut(s) 117, 130, 146, 773
Hpy8I GTNNAC 3 cut(s) 389, 433, 608
Hpy99I CGWCG 1 cut(s) 606
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 4 cut(s) 215, 292, 477, 733
HpyF10VI GCNNNNNNNGC 2 cut(s) 241, 672
HpyF3I CTNAG 3 cut(s) 65, 318, 685
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 4 cut(s) 82, 149, 532, 737
HspAI GCGC 1 cut(s) 242
KpnI GGTACC 1 cut(s) 486
Ksp22I TGATCA 1 cut(s) 126
Kzo9I GATC 5 cut(s) 11, 126, 132, 182, 196
LmnI GCTCC 1 cut(s) 61
LpnPI CCDG 7 cut(s) 85, 87, 102, 210, 285, 354, 743
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 345
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 2 cut(s) 166, 688
MalI GATC 5 cut(s) 13, 128, 134, 184, 198
MboI GATC 5 cut(s) 11, 126, 132, 182, 196
MboII GAAGA 7 cut(s) 105, 214, 347, 367, 377, 416, 747
MhlI GDGCHC 2 cut(s) 66, 427
MluCI AATT 5 cut(s) 207, 545, 697, 713, 782
MmeI TCCRAC 1 cut(s) 703
MnlI CCTC 6 cut(s) 121, 254, 400, 553, 579, 589
MseI TTAA 1 cut(s) 515
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 1 cut(s) 72
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 241, 672
NciI CCSGG 1 cut(s) 72
NdeII GATC 5 cut(s) 11, 126, 132, 182, 196
NheI GCTAGC 1 cut(s) 344
NlaIII CATG 4 cut(s) 82, 149, 532, 737
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 2 cut(s) 166, 688
NspI RCATGY 2 cut(s) 82, 737
PaeI GCATGC 2 cut(s) 82, 737
PagI TCATGA 1 cut(s) 145
PaqCI CACCTGC 1 cut(s) 290
PfeI GAWTC 2 cut(s) 149, 551
Psp124BI GAGCTC 1 cut(s) 66
PspFI CCCAGC 1 cut(s) 340
PspN4I GGNNCC 1 cut(s) 484
PspPI GGNCC 1 cut(s) 119
RsaI GTAC 4 cut(s) 174, 484, 582, 609
RsaNI GTAC 4 cut(s) 173, 483, 581, 608
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 1 cut(s) 515
Sau3AI GATC 5 cut(s) 11, 126, 132, 182, 196
Sau96I GGNCC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 2 cut(s) 66, 427
SfaNI GCATC 1 cut(s) 97
SinI GGWCC 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 771
SmoI CTYRAG 1 cut(s) 771
SphI GCATGC 2 cut(s) 82, 737
Sse9I AATT 5 cut(s) 207, 545, 697, 713, 782
SsiI CCGC 2 cut(s) 21, 589
SspMI CTAG 1 cut(s) 345
SstI GAGCTC 1 cut(s) 66
StyD4I CCNGG 1 cut(s) 70
StyI CCWWGG 1 cut(s) 486
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 178
TaqI TCGA 2 cut(s) 181, 630
TasI AATT 5 cut(s) 207, 545, 697, 713, 782
TatI WGTACW 2 cut(s) 580, 607
TfiI GAWTC 2 cut(s) 149, 551
Tru1I TTAA 1 cut(s) 515
Tru9I TTAA 1 cut(s) 515
TscAI CASTG 3 cut(s) 106, 175, 693
TseFI GTSAC 2 cut(s) 166, 688
Tsp45I GTSAC 2 cut(s) 166, 688
TspDTI ATGAA 3 cut(s) 17, 386, 417
TspGWI ACGGA 1 cut(s) 515
TspRI CASTG 3 cut(s) 106, 175, 693
VpaK11BI GGWCC 1 cut(s) 119
XapI RAATTY 1 cut(s) 207
XceI RCATGY 2 cut(s) 82, 737
XmiI GTMKAC 1 cut(s) 388
XspI CTAG 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.