Rmu_sc0006635.1_g000001

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006635.1
OG0000427
Physical Location & Seq
Forward (+)
1158 .. 1703
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006635.1_g000001.1.cds

Sequence Viewer

Length: 546 bp
atggctatctattttctcctctcgtctttgcttctaatttgcgttctatcatatggctcacaaaaactcacagccaaaaggctcaaaatcacaatcgaagaagctactgtggatgatctccagcttgctttcaggctcaagactctgacctcaagggaagttgtcctgttctacctggacaagattcgagaactgaacaaaaaccttaggggtgtcatagaggtgaaccctgatgcgttatcccaagctgatatagctgacaaagaacgcagagaacgcaaccacacactgcctaagcttcatggcattcctattctgctcaaggataacatagcaaccaaggacaaactcaacaccacagctggctcgtttgccttgttgggatccattgtgcctcgagatgcaggtgttgtttctaagctaaggaggtctggagctatcattttagggaaggctagcttgagtgaatggagcaactttagaacatcatgggcacccaacggttggagcgccagaggttcccaaggaaaggtgagtcagggttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

181

Amino Acids

20.0

Weight (kDa)

9.95

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 395
Acc36I ACCTGC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 493
AccB7I CCANNNNNTGG 1 cut(s) 504
AclWI GGATC 2 cut(s) 378, 391
AfiI CCNNNNNNNGG 2 cut(s) 504, 529
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 9 cut(s) 104, 124, 248, 257, 298, 362, 421, 437, 459
AluI AGCT 9 cut(s) 104, 124, 248, 257, 298, 362, 421, 437, 459
AlwI GGATC 2 cut(s) 378, 391
Ama87I CYCGRG 1 cut(s) 396
AspLEI GCGC 1 cut(s) 512
AsuHPI GGTGA 2 cut(s) 235, 544
AsuNHI GCTAGC 1 cut(s) 455
AvaI CYCGRG 1 cut(s) 396
AxyI CCTNAGG 1 cut(s) 206
BaeGI GKGCMC 1 cut(s) 496
BamHI GGATCC 1 cut(s) 383
BanI GGYRCC 1 cut(s) 493
BciT130I CCWGG 1 cut(s) 176
BfaI CTAG 1 cut(s) 456
BfoI RGCGCY 1 cut(s) 513
BfuAI ACCTGC 1 cut(s) 395
Bme1390I CCNGG 1 cut(s) 176
BmeT110I CYCGRG 1 cut(s) 396
BmiI GGNNCC 3 cut(s) 385, 495, 520
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 2 cut(s) 223, 391
BmtI GCTAGC 1 cut(s) 459
BpmI CTGGAG 2 cut(s) 104, 453
Bpu10I CCTNAGC 2 cut(s) 294, 422
BpuEI CTTGAG 4 cut(s) 122, 136, 305, 481
BsaJI CCNNGG 2 cut(s) 339, 523
Bsc4I CCNNNNNNNGG 2 cut(s) 504, 529
Bse21I CCTNAGG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 2 cut(s) 339, 523
BseGI GGATG 1 cut(s) 118
BseLI CCNNNNNNNGG 2 cut(s) 504, 529
BseRI GAGGAG 1 cut(s) 8
BseSI GKGCMC 1 cut(s) 496
BshNI GGYRCC 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 396
BslI CCNNNNNNNGG 2 cut(s) 504, 529
BsmI GAATGC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 396
Bsp1286I GDGCHC 1 cut(s) 496
Bsp143I GATC 2 cut(s) 115, 383
BspLI GGNNCC 3 cut(s) 385, 495, 520
BspMI ACCTGC 1 cut(s) 395
BspOI GCTAGC 1 cut(s) 459
BspPI GGATC 2 cut(s) 378, 391
BspT107I GGYRCC 1 cut(s) 493
BssECI CCNNGG 2 cut(s) 339, 523
BssMI GATC 2 cut(s) 115, 383
BssT1I CCWWGG 2 cut(s) 339, 523
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 2 cut(s) 109, 503
BstC8I GCNNGC 3 cut(s) 126, 364, 457
BstDEI CTNAG 4 cut(s) 206, 294, 417, 422
BstF5I GGATG 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 1 cut(s) 512
BstKTI GATC 2 cut(s) 118, 386
BstMBI GATC 2 cut(s) 115, 383
BstMWI GCNNNNNNNGC 2 cut(s) 254, 276
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 496
BstX2I RGATCY 1 cut(s) 383
BstYI RGATCY 1 cut(s) 383
Bsu36I CCTNAGG 1 cut(s) 206
BtsCI GGATG 1 cut(s) 118
BtsI GCAGTG 1 cut(s) 287
BtsIMutI CAGTG 1 cut(s) 287
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 3 cut(s) 126, 364, 457
CfoI GCGC 1 cut(s) 512
CviAII CATG 2 cut(s) 302, 489
DdeI CTNAG 4 cut(s) 206, 294, 417, 422
DpnI GATC 2 cut(s) 117, 385
DpnII GATC 2 cut(s) 115, 383
Eco130I CCWWGG 2 cut(s) 339, 523
Eco81I CCTNAGG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 396
EcoRII CCWGG 1 cut(s) 174
EcoT14I CCWWGG 2 cut(s) 339, 523
ErhI CCWWGG 2 cut(s) 339, 523
FaeI CATG 2 cut(s) 305, 492
FaiI YATR 7 cut(s) 52, 54, 218, 254, 303, 332, 490
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FatI CATG 2 cut(s) 301, 488
FauNDI CATATG 1 cut(s) 52
FokI GGATG 1 cut(s) 125
FspBI CTAG 1 cut(s) 456
GlaI GCGC 1 cut(s) 511
GsuI CTGGAG 2 cut(s) 104, 453
HaeII RGCGCY 1 cut(s) 513
HhaI GCGC 1 cut(s) 512
Hin1II CATG 2 cut(s) 305, 492
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 3 cut(s) 142, 184, 535
HphI GGTGA 2 cut(s) 235, 544
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 1 cut(s) 147
Hpy188III TCNNGA 4 cut(s) 139, 188, 398, 432
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 445
HpyCH4III ACNGT 2 cut(s) 109, 503
HpyCH4V TGCA 1 cut(s) 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 276
HpyF3I CTNAG 4 cut(s) 206, 294, 417, 422
Hsp92II CATG 2 cut(s) 305, 492
HspAI GCGC 1 cut(s) 510
Kzo9I GATC 2 cut(s) 115, 383
LmnI GCTCC 3 cut(s) 434, 471, 507
LweI GCATC 2 cut(s) 223, 391
MaeI CTAG 1 cut(s) 456
MalI GATC 2 cut(s) 117, 385
MboI GATC 2 cut(s) 115, 383
MboII GAAGA 1 cut(s) 110
MflI RGATCY 1 cut(s) 383
MhlI GDGCHC 1 cut(s) 496
MluCI AATT 1 cut(s) 36
MlyI GAGTC 2 cut(s) 136, 544
MmeI TCCRAC 1 cut(s) 485
MnlI CCTC 6 cut(s) 29, 160, 214, 405, 420, 509
MseI TTAA 1 cut(s) 544
MslI CAYNNNNRTG 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 176
Mva1269I GAATGC 1 cut(s) 306
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 254, 276
NdeI CATATG 1 cut(s) 52
NdeII GATC 2 cut(s) 115, 383
NheI GCTAGC 1 cut(s) 455
NlaIII CATG 2 cut(s) 305, 492
NlaIV GGNNCC 3 cut(s) 385, 495, 520
PaeR7I CTCGAG 1 cut(s) 396
PaqCI CACCTGC 1 cut(s) 395
PctI GAATGC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 184
PflMI CCANNNNNTGG 1 cut(s) 504
PleI GAGTC 2 cut(s) 136, 543
PpsI GAGTC 2 cut(s) 136, 543
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 3 cut(s) 385, 495, 520
PsuI RGATCY 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 1 cut(s) 544
Sau3AI GATC 2 cut(s) 115, 383
SchI GAGTC 2 cut(s) 136, 544
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 1 cut(s) 496
SfaNI GCATC 2 cut(s) 223, 391
Sfr274I CTCGAG 1 cut(s) 396
SlaI CTCGAG 1 cut(s) 396
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 5 cut(s) 137, 151, 320, 396, 460
SmoI CTYRAG 5 cut(s) 137, 151, 320, 396, 460
Sse9I AATT 1 cut(s) 36
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 1 cut(s) 174
StyI CCWWGG 2 cut(s) 339, 523
TaaI ACNGT 2 cut(s) 109, 503
TaqI TCGA 3 cut(s) 96, 187, 397
TasI AATT 1 cut(s) 36
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 1 cut(s) 544
Tru9I TTAA 1 cut(s) 544
TscAI CASTG 1 cut(s) 294
TspDTI ATGAA 1 cut(s) 290
TspRI CASTG 1 cut(s) 294
Van91I CCANNNNNTGG 1 cut(s) 504
XhoI CTCGAG 1 cut(s) 396
XspI CTAG 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.