Rmu_sc0039466.1_g000001

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0039466.1
OG0000427
Physical Location & Seq
Forward (+)
1 .. 1417
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0039466.1_g000001.1.cds

Sequence Viewer

Length: 913 bp
tggtactgagaccgatggctcaatactatgtccagcaagctttaactcagtagtcggcatcaaaccaaccgttggactcacgagccgagccggggtgatcccagttactcccagacaggacaccattgggactgtgacagatgctgtttatgttcttgatgcgattgtaggctatgactacaaggatcaagcaaccaaagaagcatcgaaatacattccacgcggtgggtataagcagttccttggggcttatgggctcaagggaaagagactgggaatagtgaggaatcccttcttcacctctgggagtggatctctccaaattcaagcatttgagcatcatttccaaacgctaaggcaaggaggtgctgttttggtagaccatctggaaatagcaaacattgatactatcttgaattttaatttgagtggagaagcaatagcaacagtggctgagttcaaactagccataaatgcatatctagaagaccttgtggtgtctccagttagatctttggcagatatcatagccttcaacttgaagttttctggtgtggaaatgatcaaggaatttggccaagacatttttcttgcagcacaagccacaaatgggataggcaacaatgagagggcagcattgttaaatttagcaaagctgacaaaagatggatttgagaaactaatgacatacaacaggctagatgcattggtgactcccggtgcagatattgctcctgtacttgcaattgggggattcccaggcattagtgtccctgctggatatgacaagaagggtgtgcctttcggcataacttttgggggtctaaagggatctgaaccaaagcttatacagattgcctacggtttcgagcaagctactgagattagaaagcctcccacatttctaccataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

303

Amino Acids

32.32

Weight (kDa)

6.38

Isoelectric Point (pI)

23.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 72, 225
AccI GTMKAC 1 cut(s) 379
AccII CGCG 1 cut(s) 223
AciI CCGC 1 cut(s) 223
AclWI GGATC 4 cut(s) 92, 193, 320, 839
AcoI YGGCCR 1 cut(s) 575
AcsI RAATTY 4 cut(s) 322, 416, 570, 644
AdeI CACNNNGTG 1 cut(s) 225
AfaI GTAC 2 cut(s) 5, 739
AfiI CCNNNNNNNGG 4 cut(s) 72, 92, 225, 610
AgsI TTSAA 5 cut(s) 327, 416, 461, 536, 542
AjnI CCWGG 1 cut(s) 758
AjuI GAANNNNNNNTTGG 2 cut(s) 571, 603
AluBI AGCT 4 cut(s) 40, 656, 845, 876
AluI AGCT 4 cut(s) 40, 656, 845, 876
Alw26I GTCTC 3 cut(s) 3, 263, 505
AlwI GGATC 4 cut(s) 92, 193, 320, 839
AlwNI CAGNNNCTG 2 cut(s) 144, 453
AoxI GGCC 1 cut(s) 575
ApeKI GCWGC 2 cut(s) 594, 633
ApoI RAATTY 4 cut(s) 322, 416, 570, 644
Asp700I GAANNNNTTC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 92, 718
AsuHPI GGTGA 3 cut(s) 107, 290, 722
BalI TGGCCA 1 cut(s) 577
BanII GRGCYC 1 cut(s) 259
BauI CACGAG 1 cut(s) 80
BbsI GAAGAC 1 cut(s) 493
BbvI GCAGC 2 cut(s) 606, 645
BccI CCATC 3 cut(s) 9, 391, 660
BciT130I CCWGG 1 cut(s) 760
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 92, 718
BcoDI GTCTC 3 cut(s) 3, 263, 505
BfaI CTAG 3 cut(s) 465, 483, 699
BglII AGATCT 1 cut(s) 510
BisI GCNGC 2 cut(s) 595, 634
BlsI GCNGC 2 cut(s) 596, 635
Bme1390I CCNGG 3 cut(s) 92, 718, 760
BmrFI CCNGG 3 cut(s) 92, 718, 760
BmrI ACTGGG 2 cut(s) 96, 282
BmsI GCATC 6 cut(s) 67, 131, 149, 213, 347, 692
BmuI ACTGGG 2 cut(s) 96, 282
BpiI GAAGAC 1 cut(s) 493
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpmI CTGGAG 1 cut(s) 487
Bpu10I CCTNAGC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 243
BpuMI CCSGG 2 cut(s) 92, 718
BsaI GGTCTC 1 cut(s) 3
BsaJI CCNNGG 3 cut(s) 91, 242, 758
BsaXI ACNNNNNCTCC 2 cut(s) 355, 385
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 92, 225, 610
Bse1I ACTGG 3 cut(s) 102, 277, 504
BseBI CCWGG 1 cut(s) 760
BseDI CCNNGG 3 cut(s) 91, 242, 758
BseLI CCNNNNNNNGG 4 cut(s) 72, 92, 225, 610
BseMII CTCAG 3 cut(s) 61, 445, 871
BseNI ACTGG 3 cut(s) 102, 277, 504
BseXI GCAGC 2 cut(s) 606, 645
BsgI GTGCAG 1 cut(s) 742
Bsh1236I CGCG 1 cut(s) 223
BshFI GGCC 1 cut(s) 577
BsiSI CCGG 2 cut(s) 91, 718
BslFI GGGAC 2 cut(s) 143, 756
BslI CCNNNNNNNGG 4 cut(s) 72, 92, 225, 610
BsmAI GTCTC 3 cut(s) 3, 263, 505
BsmFI GGGAC 2 cut(s) 143, 756
BsnI GGCC 1 cut(s) 577
Bso31I GGTCTC 1 cut(s) 3
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 6 cut(s) 97, 185, 312, 510, 562, 831
BspACI CCGC 1 cut(s) 223
BspANI GGCC 1 cut(s) 577
BspCNI CTCAG 3 cut(s) 60, 446, 872
BspFNI CGCG 1 cut(s) 223
BspPI GGATC 4 cut(s) 92, 193, 320, 839
BspTNI GGTCTC 1 cut(s) 3
BsrI ACTGG 3 cut(s) 102, 277, 504
BssECI CCNNGG 3 cut(s) 91, 242, 758
BssMI GATC 6 cut(s) 97, 185, 312, 510, 562, 831
BssSI CACGAG 1 cut(s) 80
BssT1I CCWWGG 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 80
Bst2UI CCWGG 1 cut(s) 760
Bst4CI ACNGT 4 cut(s) 71, 134, 449, 864
BstAPI GCANNNNNTGC 1 cut(s) 729
BstC8I GCNNGC 2 cut(s) 38, 874
BstDEI CTNAG 5 cut(s) 7, 47, 354, 454, 880
BstFNI CGCG 1 cut(s) 223
BstKTI GATC 6 cut(s) 100, 188, 315, 513, 565, 834
BstMAI GTCTC 3 cut(s) 3, 263, 505
BstMBI GATC 6 cut(s) 97, 185, 312, 510, 562, 831
BstMWI GCNNNNNNNGC 4 cut(s) 450, 474, 600, 729
BstNI CCWGG 1 cut(s) 760
BstSCI CCNGG 3 cut(s) 90, 716, 758
BstUI CGCG 1 cut(s) 223
BstV1I GCAGC 2 cut(s) 606, 645
BstV2I GAAGAC 1 cut(s) 493
BstX2I RGATCY 3 cut(s) 312, 510, 831
BstYI RGATCY 3 cut(s) 312, 510, 831
BsuRI GGCC 1 cut(s) 577
BtsIMutI CAGTG 1 cut(s) 454
Cac8I GCNNGC 2 cut(s) 38, 874
CaiI CAGNNNCTG 2 cut(s) 144, 453
Csp6I GTAC 2 cut(s) 4, 738
CviQI GTAC 2 cut(s) 4, 738
DdeI CTNAG 5 cut(s) 7, 47, 354, 454, 880
DpnI GATC 6 cut(s) 99, 187, 314, 512, 564, 833
DpnII GATC 6 cut(s) 97, 185, 312, 510, 562, 831
DraIII CACNNNGTG 1 cut(s) 225
EaeI YGGCCR 1 cut(s) 575
Eco130I CCWWGG 1 cut(s) 242
Eco24I GRGCYC 1 cut(s) 259
Eco31I GGTCTC 1 cut(s) 3
Eco32I GATATC 1 cut(s) 524
EcoRII CCWGG 1 cut(s) 758
EcoRV GATATC 1 cut(s) 524
EcoT14I CCWWGG 1 cut(s) 242
EcoT22I ATGCAT 2 cut(s) 479, 707
EcoT38I GRGCYC 1 cut(s) 259
ErhI CCWWGG 1 cut(s) 242
FaqI GGGAC 2 cut(s) 143, 756
FbaI TGATCA 1 cut(s) 562
FblI GTMKAC 1 cut(s) 379
Fnu4HI GCNGC 2 cut(s) 595, 634
FriOI GRGCYC 1 cut(s) 259
Fsp4HI GCNGC 2 cut(s) 595, 634
FspBI CTAG 3 cut(s) 465, 483, 699
GluI GCNGC 2 cut(s) 595, 634
GsuI CTGGAG 1 cut(s) 487
HaeIII GGCC 1 cut(s) 577
HapII CCGG 2 cut(s) 91, 718
HindIII AAGCTT 2 cut(s) 38, 843
HinfI GANTC 4 cut(s) 76, 287, 713, 754
HpaII CCGG 2 cut(s) 91, 718
HphI GGTGA 3 cut(s) 107, 290, 722
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 1 cut(s) 836
Hpy188III TCNNGA 5 cut(s) 80, 156, 387, 413, 483
Hpy8I GTNNAC 1 cut(s) 380
HpyAV CCTTC 3 cut(s) 302, 542, 785
HpyCH4III ACNGT 4 cut(s) 71, 134, 449, 864
HpyCH4V TGCA 5 cut(s) 477, 594, 705, 723, 744
HpyF10VI GCNNNNNNNGC 4 cut(s) 450, 474, 600, 729
HpyF3I CTNAG 5 cut(s) 7, 47, 354, 454, 880
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 6 cut(s) 97, 185, 312, 510, 562, 831
LmnI GCTCC 1 cut(s) 737
Lsp1109I GCAGC 2 cut(s) 606, 645
LweI GCATC 6 cut(s) 67, 131, 149, 213, 347, 692
MaeI CTAG 3 cut(s) 465, 483, 699
MaeIII GTNAC 3 cut(s) 104, 134, 710
MalI GATC 6 cut(s) 99, 187, 314, 512, 564, 833
MboI GATC 6 cut(s) 97, 185, 312, 510, 562, 831
MboII GAAGA 2 cut(s) 287, 498
MfeI CAATTG 1 cut(s) 745
MflI RGATCY 3 cut(s) 312, 510, 831
MhlI GDGCHC 1 cut(s) 259
MlsI TGGCCA 1 cut(s) 577
MluCI AATT 6 cut(s) 322, 416, 422, 570, 644, 745
MluNI TGGCCA 1 cut(s) 577
MlyI GAGTC 2 cut(s) 70, 707
MmeI TCCRAC 1 cut(s) 53
MnlI CCTC 5 cut(s) 277, 311, 357, 622, 904
Mox20I TGGCCA 1 cut(s) 577
Mph1103I ATGCAT 2 cut(s) 479, 707
MroXI GAANNNNTTC 1 cut(s) 291
MscI TGGCCA 1 cut(s) 577
MseI TTAA 3 cut(s) 43, 421, 642
Msp20I TGGCCA 1 cut(s) 577
MspI CCGG 2 cut(s) 91, 718
MspR9I CCNGG 3 cut(s) 92, 718, 760
MunI CAATTG 1 cut(s) 745
MvaI CCWGG 1 cut(s) 760
MvnI CGCG 1 cut(s) 223
MwoI GCNNNNNNNGC 4 cut(s) 450, 474, 600, 729
NciI CCSGG 2 cut(s) 92, 718
NdeII GATC 6 cut(s) 97, 185, 312, 510, 562, 831
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 134, 710
NsiI ATGCAT 2 cut(s) 479, 707
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 2 cut(s) 287, 754
PflMI CCANNNNNTGG 2 cut(s) 72, 225
PkrI GCNGC 2 cut(s) 596, 635
PleI GAGTC 2 cut(s) 70, 707
PpsI GAGTC 2 cut(s) 70, 707
Psp6I CCWGG 1 cut(s) 758
PspGI CCWGG 1 cut(s) 758
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PstNI CAGNNNCTG 2 cut(s) 144, 453
PsuI RGATCY 3 cut(s) 312, 510, 831
RsaI GTAC 2 cut(s) 5, 739
RsaNI GTAC 2 cut(s) 4, 738
SaqAI TTAA 3 cut(s) 43, 421, 642
SatI GCNGC 2 cut(s) 595, 634
Sau3AI GATC 6 cut(s) 97, 185, 312, 510, 562, 831
SchI GAGTC 2 cut(s) 70, 707
ScrFI CCNGG 3 cut(s) 92, 718, 760
SduI GDGCHC 1 cut(s) 259
SetI ASST 7 cut(s) 42, 303, 368, 493, 658, 847, 878
SfaNI GCATC 6 cut(s) 67, 131, 149, 213, 347, 692
SmlI CTYRAG 1 cut(s) 258
SmoI CTYRAG 1 cut(s) 258
Sse9I AATT 6 cut(s) 322, 416, 422, 570, 644, 745
SsiI CCGC 1 cut(s) 223
SspMI CTAG 3 cut(s) 465, 483, 699
StyD4I CCNGG 3 cut(s) 90, 716, 758
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 4 cut(s) 71, 134, 449, 864
TaqI TCGA 2 cut(s) 207, 868
TaqII GACCGA 1 cut(s) 27
TasI AATT 6 cut(s) 322, 416, 422, 570, 644, 745
TatI WGTACW 1 cut(s) 737
TfiI GAWTC 2 cut(s) 287, 754
Tru1I TTAA 3 cut(s) 43, 421, 642
Tru9I TTAA 3 cut(s) 43, 421, 642
TscAI CASTG 1 cut(s) 454
TseFI GTSAC 2 cut(s) 134, 710
TseI GCWGC 2 cut(s) 594, 633
Tsp45I GTSAC 2 cut(s) 134, 710
TspRI CASTG 1 cut(s) 454
Van91I CCANNNNNTGG 2 cut(s) 72, 225
XapI RAATTY 4 cut(s) 322, 416, 570, 644
XbaI TCTAGA 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 379
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 3 cut(s) 465, 483, 699
Zsp2I ATGCAT 2 cut(s) 479, 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.