Rorug05G0510200

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000427
Physical Location & Seq
Forward (+)
69424757 .. 69425733
977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0510200.1

Sequence Viewer

Length: 735 bp
ATGGAGGCCCCCCTTTCATCATGGCCATCATCAATTGAAGATCGTTGTACTGCTGTGGTGCATCTTGCTTTTTACGGTTGCGATCTGAAGGAGTTATCGGATGCCATTGCTTATTTTCGTTCGTTAAAAGTATTGAATTTGTCAGGAAATAAGAATCTGACAAGCTTGCCTGCAGCCATGAATCAACTGGGTTGCTTAGAACATCTTGAATTGAAAGATTGCAACAGTCTGAGATCAATACCAGAGCTTTCATCAAGAATAAGTTACATAAATGCGCATAATTGCACAGCTTTGGAAGCTGTTTCAACACCACAGTCTCCCTACGATATCGGTCGGTGCTTCAACTTCTCTAACTGCCATCAGCTGGTACAGAAAGATTTTTTCAGAGATATTGTAGAAACTCATTCCCCTCCTCAGGGTAATTGTTCCCGACCCTTTTATTTCTCTATTCCTGGAAGTGAAATTCCAGAGCAGTTCAACCATCAGTGGAGGGGGACTTCCGTAACTGTCCAGCTACCACAAAATTGGTTTGACAACAAGTTTTTGGGGTTTGCTATATGCGCGGTTACTAACAAGCCCCAAAGTGTTAATTATTATAGATTGACTGCTCGATGTTTCTGTACCTTCAAAGGAGAACATTGCCAGTACCAAATCTCTATGAGGGCAATGAGTCATTTTGGAAGGGGAAAACTCTGGAAAGTTGGACACAAGAAGTCAGAGTTACATCGGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

244

Amino Acids

27.75

Weight (kDa)

8.58

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 150 - 195 2.7e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 276
AccB7I CCANNNNNTGG 1 cut(s) 364
AccII CGCG 1 cut(s) 563
AciI CCGC 1 cut(s) 563
AcoI YGGCCR 1 cut(s) 23
AcsI RAATTY 2 cut(s) 136, 462
AcuI CTGAAG 1 cut(s) 107
AfaI GTAC 4 cut(s) 49, 369, 622, 647
AfiI CCNNNNNNNGG 3 cut(s) 364, 415, 416
AgsI TTSAA 8 cut(s) 38, 136, 209, 214, 306, 343, 478, 628
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 6 cut(s) 165, 247, 290, 299, 364, 514
AluI AGCT 6 cut(s) 165, 247, 290, 299, 364, 514
Alw26I GTCTC 1 cut(s) 321
AoxI GGCC 2 cut(s) 6, 23
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 2 cut(s) 136, 462
AspLEI GCGC 2 cut(s) 277, 563
AspS9I GGNCC 1 cut(s) 7
AxyI CCTNAGG 1 cut(s) 414
BalI TGGCCA 1 cut(s) 25
BbvI GCAGC 1 cut(s) 185
BccI CCATC 3 cut(s) 34, 366, 489
BciT130I CCWGG 1 cut(s) 453
BcoDI GTCTC 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 171
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 453
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 453
BmrI ACTGGG 1 cut(s) 197
BmsI GCATC 2 cut(s) 70, 91
BmuI ACTGGG 1 cut(s) 197
Bsc4I CCNNNNNNNGG 3 cut(s) 364, 415, 416
Bse1I ACTGG 2 cut(s) 192, 643
Bse21I CCTNAGG 1 cut(s) 414
Bse3DI GCAATG 3 cut(s) 105, 637, 672
BseBI CCWGG 1 cut(s) 453
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 3 cut(s) 364, 415, 416
BseMI GCAATG 3 cut(s) 105, 637, 672
BseMII CTCAG 2 cut(s) 221, 428
BseNI ACTGG 2 cut(s) 192, 643
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 1 cut(s) 185
Bsh1236I CGCG 1 cut(s) 563
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 2 cut(s) 8, 25
BsiEI CGRYCG 1 cut(s) 334
BslFI GGGAC 1 cut(s) 508
BslI CCNNNNNNNGG 3 cut(s) 364, 415, 416
BsmAI GTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 508
BsnI GGCC 2 cut(s) 8, 25
Bsp143I GATC 3 cut(s) 40, 82, 233
BspACI CCGC 1 cut(s) 563
BspANI GGCC 2 cut(s) 8, 25
BspCNI CTCAG 2 cut(s) 222, 427
BspFNI CGCG 1 cut(s) 563
BspLI GGNNCC 1 cut(s) 9
BspMAI CTGCAG 1 cut(s) 175
BsrDI GCAATG 3 cut(s) 105, 637, 672
BsrI ACTGG 2 cut(s) 192, 643
BssMI GATC 3 cut(s) 40, 82, 233
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 4 cut(s) 77, 227, 315, 508
BstC8I GCNNGC 2 cut(s) 167, 171
BstDEI CTNAG 3 cut(s) 196, 230, 414
BstF5I GGATG 1 cut(s) 106
BstFNI CGCG 1 cut(s) 563
BstHHI GCGC 2 cut(s) 277, 563
BstKTI GATC 3 cut(s) 43, 85, 236
BstMAI GTCTC 1 cut(s) 321
BstMBI GATC 3 cut(s) 40, 82, 233
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 2 cut(s) 296, 560
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 171
BstUI CGCG 1 cut(s) 563
BstV1I GCAGC 1 cut(s) 185
BstXI CCANNNNNNTGG 1 cut(s) 525
Bsu36I CCTNAGG 1 cut(s) 414
BsuRI GGCC 2 cut(s) 8, 25
BtsCI GGATG 1 cut(s) 106
BtsIMutI CAGTG 1 cut(s) 491
Cac8I GCNNGC 2 cut(s) 167, 171
CfoI GCGC 2 cut(s) 277, 563
Cfr13I GGNCC 1 cut(s) 7
Csp6I GTAC 4 cut(s) 48, 368, 621, 646
CviAII CATG 2 cut(s) 21, 178
CviQI GTAC 4 cut(s) 48, 368, 621, 646
DdeI CTNAG 3 cut(s) 196, 230, 414
DpnI GATC 3 cut(s) 42, 84, 235
DpnII GATC 3 cut(s) 40, 82, 233
EaeI YGGCCR 1 cut(s) 23
Eco32I GATATC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 107
Eco81I CCTNAGG 1 cut(s) 414
EcoO109I RGGNCCY 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 451
EcoRV GATATC 1 cut(s) 328
FaeI CATG 2 cut(s) 24, 181
FaiI YATR 8 cut(s) 22, 179, 269, 279, 557, 559, 597, 659
FaqI GGGAC 1 cut(s) 508
FatI CATG 2 cut(s) 20, 177
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 174
FspAI RTGCGCAY 1 cut(s) 276
FspI TGCGCA 1 cut(s) 276
GlaI GCGC 2 cut(s) 276, 562
GluI GCNGC 1 cut(s) 174
HaeIII GGCC 2 cut(s) 8, 25
HhaI GCGC 2 cut(s) 277, 563
Hin1II CATG 2 cut(s) 24, 181
Hin6I GCGC 2 cut(s) 275, 561
HinP1I GCGC 2 cut(s) 275, 561
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 3 cut(s) 154, 181, 670
Hpy188I TCNGA 7 cut(s) 87, 100, 159, 231, 386, 718, 734
Hpy188III TCNNGA 6 cut(s) 144, 206, 255, 429, 467, 694
HpyAV CCTTC 3 cut(s) 82, 634, 675
HpyCH4III ACNGT 4 cut(s) 77, 227, 315, 508
HpyCH4V TGCA 4 cut(s) 61, 173, 222, 285
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 560
HpyF3I CTNAG 3 cut(s) 196, 230, 414
Hsp92II CATG 2 cut(s) 24, 181
HspAI GCGC 2 cut(s) 275, 561
Kzo9I GATC 3 cut(s) 40, 82, 233
Lsp1109I GCAGC 1 cut(s) 185
LweI GCATC 2 cut(s) 70, 91
MaeIII GTNAC 4 cut(s) 263, 502, 565, 720
MalI GATC 3 cut(s) 42, 84, 235
MboI GATC 3 cut(s) 40, 82, 233
MboII GAAGA 1 cut(s) 50
MfeI CAATTG 1 cut(s) 33
MlsI TGGCCA 1 cut(s) 25
MluCI AATT 8 cut(s) 33, 136, 209, 280, 421, 462, 523, 589
MluNI TGGCCA 1 cut(s) 25
MlyI GAGTC 1 cut(s) 679
MmeI TCCRAC 1 cut(s) 682
MnlI CCTC 4 cut(s) 420, 423, 483, 654
Mox20I TGGCCA 1 cut(s) 25
MscI TGGCCA 1 cut(s) 25
MseI TTAA 2 cut(s) 125, 588
Msp20I TGGCCA 1 cut(s) 25
MspA1I CMGCKG 1 cut(s) 364
MspR9I CCNGG 1 cut(s) 453
MunI CAATTG 1 cut(s) 33
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 1 cut(s) 563
MwoI GCNNNNNNNGC 2 cut(s) 296, 560
NdeII GATC 3 cut(s) 40, 82, 233
NlaIII CATG 2 cut(s) 24, 181
NlaIV GGNNCC 1 cut(s) 9
NsbI TGCGCA 1 cut(s) 276
PfeI GAWTC 2 cut(s) 154, 181
PflMI CCANNNNNTGG 1 cut(s) 364
PfoI TCCNGGA 1 cut(s) 451
PkrI GCNGC 1 cut(s) 175
PleI GAGTC 1 cut(s) 678
PpsI GAGTC 1 cut(s) 678
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 7
PstI CTGCAG 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 364
RsaI GTAC 4 cut(s) 49, 369, 622, 647
RsaNI GTAC 4 cut(s) 48, 368, 621, 646
SaqAI TTAA 2 cut(s) 125, 588
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 3 cut(s) 40, 82, 233
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 1 cut(s) 453
SetI ASST 7 cut(s) 167, 249, 292, 301, 366, 516, 626
SfaNI GCATC 2 cut(s) 70, 91
SfcI CTRYAG 1 cut(s) 171
Sse9I AATT 8 cut(s) 33, 136, 209, 280, 421, 462, 523, 589
SsiI CCGC 1 cut(s) 563
StyD4I CCNGG 1 cut(s) 451
TaaI ACNGT 4 cut(s) 77, 227, 315, 508
TaqI TCGA 1 cut(s) 610
TaqII GACCGA 1 cut(s) 320
TasI AATT 8 cut(s) 33, 136, 209, 280, 421, 462, 523, 589
TatI WGTACW 1 cut(s) 47
TfiI GAWTC 2 cut(s) 154, 181
Tru1I TTAA 2 cut(s) 125, 588
Tru9I TTAA 2 cut(s) 125, 588
TscAI CASTG 1 cut(s) 491
TseI GCWGC 1 cut(s) 173
TspDTI ATGAA 3 cut(s) 6, 194, 240
TspGWI ACGGA 1 cut(s) 490
TspRI CASTG 1 cut(s) 491
Van91I CCANNNNNTGG 1 cut(s) 364
XapI RAATTY 2 cut(s) 136, 462
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.