Rroxscaffold_7G00215560

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000427
Physical Location & Seq
Forward (+)
66186296 .. 66188500
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215560.1

Sequence Viewer

Length: 783 bp
ATGGTTTCAGTTTATGTTATGTTGTGTATATTGTGGAATGTTCTTGCTGGACATGGAATTGAGTATGTTGACAATGATAACACAGTTAAGGCCATCAATTTTATCAACGCTAGTGTTGGTGAATCTAAAACACCAATACCTCCCCACGAAAGAACTTCTACTGCAACTCCGGAAGTTCAAGGAACTCAAGGAAGTCAAGCAACCGAGCTTGAAGATGATGGAAGTGTGGAAAGCATCCTTGAGGCGTTGAAACGAAAAGAAAGAACCGAACCCACCCCTAATTCTTCTAGCGATGAAGATACTGCATTGTCTGTTGAGTTCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGCTGCTATAGCCTTCAACAACAAACACCCGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACGTATTTTTAGCTGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCCGGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGCTGTGGTGACTCCTGACGCAGGTGTTTCAAGAGTACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCCGGATACAAAAACAATGGGAGACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCTACTAAGATTAGGAAGCCTCCTTCATTCAAAGGCTTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

260

Amino Acids

28.01

Weight (kDa)

7.71

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 575
Acc36I ACCTGC 1 cut(s) 575
AccIII TCCGGA 1 cut(s) 169
AclWI GGATC 1 cut(s) 366
AcuI CTGAAG 1 cut(s) 480
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 2 cut(s) 415, 584
AjnI CCWGG 1 cut(s) 619
AluBI AGCT 7 cut(s) 208, 383, 455, 559, 665, 706, 737
AluI AGCT 7 cut(s) 208, 383, 455, 559, 665, 706, 737
Alw26I GTCTC 1 cut(s) 652
AlwI GGATC 1 cut(s) 366
Aor13HI TCCGGA 1 cut(s) 169
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 3 cut(s) 380, 383, 455
AsuHPI GGTGA 3 cut(s) 131, 556, 583
BanII GRGCYC 1 cut(s) 619
BbvI GCAGC 3 cut(s) 370, 392, 442
BccI CCATC 2 cut(s) 101, 212
BciT130I CCWGG 1 cut(s) 621
BciVI GTATCC 1 cut(s) 635
BcoDI GTCTC 1 cut(s) 652
BfaI CTAG 3 cut(s) 111, 288, 560
BfmI CTRYAG 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 575
BfuI GTATCC 1 cut(s) 635
BisI GCNGC 3 cut(s) 381, 384, 456
BlsI GCNGC 3 cut(s) 382, 385, 457
BmcAI AGTACT 1 cut(s) 600
Bme1390I CCNGG 1 cut(s) 621
BmiI GGNNCC 1 cut(s) 616
BmrFI CCNGG 1 cut(s) 621
BmrI ACTGGG 1 cut(s) 359
BmsI GCATC 2 cut(s) 243, 553
BmuI ACTGGG 1 cut(s) 359
BpuEI CTTGAG 2 cut(s) 171, 260
BsaI GGTCTC 1 cut(s) 652
BsaJI CCNNGG 2 cut(s) 375, 619
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 2 cut(s) 151, 181
Bsc4I CCNNNNNNNGG 2 cut(s) 415, 584
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 2 cut(s) 365, 420
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 1 cut(s) 621
BseDI CCNNGG 2 cut(s) 375, 619
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 2 cut(s) 415, 584
BseNI ACTGG 2 cut(s) 365, 420
BseXI GCAGC 3 cut(s) 370, 392, 442
BshFI GGCC 1 cut(s) 92
BsiSI CCGG 3 cut(s) 170, 494, 639
BslI CCNNNNNNNGG 2 cut(s) 415, 584
BsmAI GTCTC 1 cut(s) 652
BsnI GGCC 1 cut(s) 92
Bso31I GGTCTC 1 cut(s) 652
Bsp1286I GDGCHC 1 cut(s) 619
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 2 cut(s) 371, 423
BspANI GGCC 1 cut(s) 92
BspEI TCCGGA 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 616
BspMI ACCTGC 1 cut(s) 575
BspPI GGATC 1 cut(s) 366
BspTNI GGTCTC 1 cut(s) 652
BsrFI RCCGGY 1 cut(s) 493
BsrI ACTGG 2 cut(s) 365, 420
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 2 cut(s) 375, 619
BssMI GATC 2 cut(s) 371, 423
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 621
Bst4CI ACNGT 1 cut(s) 85
BstC8I GCNNGC 2 cut(s) 495, 735
BstDEI CTNAG 1 cut(s) 741
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 1 cut(s) 234
BstKTI GATC 2 cut(s) 374, 426
BstMAI GTCTC 1 cut(s) 652
BstMBI GATC 2 cut(s) 371, 423
BstMWI GCNNNNNNNGC 2 cut(s) 389, 461
BstNI CCWGG 1 cut(s) 621
BstSCI CCNGG 1 cut(s) 619
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 3 cut(s) 370, 392, 442
BsuI GTATCC 1 cut(s) 635
BsuRI GGCC 1 cut(s) 92
BtgZI GCGATG 1 cut(s) 306
BtsCI GGATG 1 cut(s) 234
BtsIMutI CAGTG 1 cut(s) 372
BveI ACCTGC 1 cut(s) 575
Cac8I GCNNGC 2 cut(s) 495, 735
Cfr10I RCCGGY 1 cut(s) 493
CseI GACGC 1 cut(s) 590
Csp6I GTAC 1 cut(s) 599
CviAII CATG 1 cut(s) 53
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 1 cut(s) 741
DpnI GATC 2 cut(s) 373, 425
DpnII GATC 2 cut(s) 371, 423
Eco130I CCWWGG 1 cut(s) 375
Eco24I GRGCYC 1 cut(s) 619
Eco31I GGTCTC 1 cut(s) 652
Eco57I CTGAAG 1 cut(s) 480
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoRII CCWGG 1 cut(s) 619
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 619
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 1 cut(s) 56
FatI CATG 1 cut(s) 52
FauNDI CATATG 1 cut(s) 721
Fnu4HI GCNGC 3 cut(s) 381, 384, 456
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 619
Fsp4HI GCNGC 3 cut(s) 381, 384, 456
FspBI CTAG 3 cut(s) 111, 288, 560
GluI GCNGC 3 cut(s) 381, 384, 456
HaeIII GGCC 1 cut(s) 92
HapII CCGG 3 cut(s) 170, 494, 639
HgaI GACGC 1 cut(s) 590
Hin1II CATG 1 cut(s) 56
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 3 cut(s) 122, 505, 574
HpaII CCGG 3 cut(s) 170, 494, 639
HphI GGTGA 3 cut(s) 131, 556, 583
Hpy166II GTNNAC 3 cut(s) 70, 519, 683
Hpy188I TCNGA 1 cut(s) 697
Hpy188III TCNNGA 4 cut(s) 170, 578, 594, 712
Hpy8I GTNNAC 3 cut(s) 70, 519, 683
HpyAV CCTTC 3 cut(s) 340, 403, 768
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 6 cut(s) 164, 305, 338, 512, 605, 719
HpyF10VI GCNNNNNNNGC 2 cut(s) 389, 461
HpyF3I CTNAG 1 cut(s) 741
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 169
KroI GCCGGC 1 cut(s) 493
KroNI GCCGGC 1 cut(s) 495
Kzo9I GATC 2 cut(s) 371, 423
LmnI GCTCC 1 cut(s) 614
Lsp1109I GCAGC 3 cut(s) 370, 392, 442
LweI GCATC 2 cut(s) 243, 553
MaeI CTAG 3 cut(s) 111, 288, 560
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 544, 571
MalI GATC 2 cut(s) 373, 425
MboI GATC 2 cut(s) 371, 423
MboII GAAGA 4 cut(s) 224, 276, 308, 565
MfeI CAATTG 1 cut(s) 606
MhlI GDGCHC 1 cut(s) 619
MluCI AATT 5 cut(s) 57, 97, 280, 539, 606
MlyI GAGTC 1 cut(s) 568
MnlI CCTC 3 cut(s) 150, 235, 765
Mph1103I ATGCAT 1 cut(s) 340
MroI TCCGGA 1 cut(s) 169
MroNI GCCGGC 1 cut(s) 493
MseI TTAA 2 cut(s) 87, 332
MspA1I CMGCKG 2 cut(s) 383, 665
MspI CCGG 3 cut(s) 170, 494, 639
MspR9I CCNGG 1 cut(s) 621
MunI CAATTG 1 cut(s) 606
MvaI CCWGG 1 cut(s) 621
MwoI GCNNNNNNNGC 2 cut(s) 389, 461
NaeI GCCGGC 1 cut(s) 495
NdeI CATATG 1 cut(s) 721
NdeII GATC 2 cut(s) 371, 423
NgoMIV GCCGGC 1 cut(s) 493
NlaIII CATG 1 cut(s) 56
NlaIV GGNNCC 1 cut(s) 616
NmuCI GTSAC 2 cut(s) 544, 571
NsiI ATGCAT 1 cut(s) 340
PaqCI CACCTGC 1 cut(s) 575
PdiI GCCGGC 1 cut(s) 495
PfeI GAWTC 2 cut(s) 122, 505
PkrI GCNGC 3 cut(s) 382, 385, 457
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 619
PspGI CCWGG 1 cut(s) 619
PspN4I GGNNCC 1 cut(s) 616
PvuII CAGCTG 2 cut(s) 383, 665
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
SaqAI TTAA 2 cut(s) 87, 332
SatI GCNGC 3 cut(s) 381, 384, 456
Sau3AI GATC 2 cut(s) 371, 423
ScaI AGTACT 1 cut(s) 600
SchI GAGTC 1 cut(s) 568
ScrFI CCNGG 1 cut(s) 621
SduI GDGCHC 1 cut(s) 619
SfaNI GCATC 2 cut(s) 243, 553
SfcI CTRYAG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 186, 239
SmoI CTYRAG 2 cut(s) 186, 239
Sse9I AATT 5 cut(s) 57, 97, 280, 539, 606
SspMI CTAG 3 cut(s) 111, 288, 560
StyD4I CCNGG 1 cut(s) 619
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 1 cut(s) 85
TaiI ACGT 1 cut(s) 447
TaqI TCGA 1 cut(s) 711
TasI AATT 5 cut(s) 57, 97, 280, 539, 606
TatI WGTACW 1 cut(s) 598
TfiI GAWTC 2 cut(s) 122, 505
Tru1I TTAA 2 cut(s) 87, 332
Tru9I TTAA 2 cut(s) 87, 332
TscAI CASTG 1 cut(s) 372
TseFI GTSAC 2 cut(s) 544, 571
TseI GCWGC 3 cut(s) 380, 383, 455
Tsp45I GTSAC 2 cut(s) 544, 571
TspDTI ATGAA 2 cut(s) 309, 750
TspRI CASTG 1 cut(s) 372
XagI CCTNNNNNAGG 1 cut(s) 582
XspI CTAG 3 cut(s) 111, 288, 560
ZrmI AGTACT 1 cut(s) 600
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.