Rroxscaffold_7G00215530

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000427
Physical Location & Seq
Forward (+)
66142756 .. 66144626
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215530.1

Sequence Viewer

Length: 1110 bp
ATGATAATTTCCTCATCCACTGCCATGGCGAAATTCCCTCACATCTCATGCTTATTGCTTCTCTTCACATTGTCATCTCTTCCAGGGACATTCGCAGCCCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTCAAGCACAACCAACTCACCTCGAGGCAACTTGACCAAGGCACCAGTGCACTACTCTCTAAGTTGCATGGGATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGACAAGATGAACACCACCGCCGGCTCTTTTGCGCTGTTTGGATCTGTCGTGCATCGTGATGCCGGCGTGGTGGAGAAGTTGCGGAAAGCAGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGTCTACTAAAACCCCCTCAGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACGTACACTTTCTCAGACCCTTGCGGCTCAAGCACAGTTTATGCATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGTGGAAAAAGAATACGGATATTGGGAGCCTTTCATGACTTTGGCAATGAAACTTCCTTGGCTCAAACTTTTGAGAAACACAACACTCTGAGGAAAGGAGGAGCTGTTTTGGTAGACAATTTGGAAATTGCTAATTTGACTGAAATCTATTCTAGAAGCGATGAAGATACTGCATTGTCTATTGAGTGCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCAGAAACTGGAAAATATCAAGGAGTATGGGCAAGACCGATTTTTAGCAGCTGAAGCAACAAATGGGTTTGGAAAAACAGATAAGCTGGCATTGTTGAATCTTGCAAGGTGGACAATAAATGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGCTGTGGTGTCTCCTTACGCAAGTTTGTCACGTGTACTTGCAGTTGGGGGAGCCCCAGGTCTAATAGTTCCAGCTGGATACGAAAACAATGGGGAACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGACCAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

369

Amino Acids

39.96

Weight (kDa)

9.62

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 46 - 137 6.6e-20 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 188
AccB7I CCANNNNNTGG 1 cut(s) 817
AccI GTMKAC 2 cut(s) 398, 648
AccII CGCG 1 cut(s) 525
AciI CCGC 5 cut(s) 100, 273, 337, 432, 469
AclWI GGATC 2 cut(s) 304, 768
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 882
AcvI CACGTG 1 cut(s) 998
AfaI GTAC 2 cut(s) 450, 1002
AfiI CCNNNNNNNGG 3 cut(s) 417, 500, 817
AflIII ACRYGT 1 cut(s) 997
AgsI TTSAA 5 cut(s) 151, 445, 748, 799, 907
AjnI CCWGG 2 cut(s) 82, 1021
AluBI AGCT 9 cut(s) 142, 372, 638, 860, 895, 961, 1040, 1067, 1107
AluI AGCT 9 cut(s) 142, 372, 638, 860, 895, 961, 1040, 1067, 1107
Alw21I GWGCWC 1 cut(s) 199
Alw26I GTCTC 1 cut(s) 981
Alw44I GTGCAC 1 cut(s) 195
AlwI GGATC 2 cut(s) 304, 768
AlwNI CAGNNNCTG 1 cut(s) 817
Ama87I CYCGRG 1 cut(s) 169
ApaLI GTGCAC 1 cut(s) 195
ApeKI GCWGC 2 cut(s) 95, 857
ApoI RAATTY 1 cut(s) 32
AspLEI GCGC 3 cut(s) 289, 425, 525
AsuHPI GGTGA 2 cut(s) 157, 958
AsuNHI GCTAGC 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 169
AxyI CCTNAGG 1 cut(s) 412
BaeGI GKGCMC 1 cut(s) 199
BanI GGYRCC 1 cut(s) 188
BanII GRGCYC 1 cut(s) 1021
BbrPI CACGTG 1 cut(s) 998
Bbv12I GWGCWC 1 cut(s) 199
BbvI GCAGC 2 cut(s) 107, 869
BciT130I CCWGG 2 cut(s) 84, 1023
BciVI GTATCC 1 cut(s) 1037
BcoDI GTCTC 1 cut(s) 981
BfaI CTAG 3 cut(s) 369, 687, 962
BfoI RGCGCY 1 cut(s) 426
BfuI GTATCC 1 cut(s) 1037
BisI GCNGC 3 cut(s) 96, 470, 858
BlsI GCNGC 3 cut(s) 97, 471, 859
Bme1390I CCNGG 2 cut(s) 84, 1023
BmeT110I CYCGRG 1 cut(s) 169
BmiI GGNNCC 4 cut(s) 190, 562, 1018, 1062
BmrFI CCNGG 2 cut(s) 84, 1023
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 3 cut(s) 304, 316, 955
BmtI GCTAGC 1 cut(s) 372
BmuI ACTGGG 1 cut(s) 761
BpmI CTGGAG 1 cut(s) 122
BpuEI CTTGAG 2 cut(s) 394, 458
BsaAI YACGTR 1 cut(s) 998
BsaJI CCNNGG 7 cut(s) 24, 83, 184, 252, 591, 777, 1021
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bsc4I CCNNNNNNNGG 3 cut(s) 417, 500, 817
Bse118I RCCGGY 2 cut(s) 275, 317
Bse1I ACTGG 3 cut(s) 192, 767, 822
Bse21I CCTNAGG 1 cut(s) 412
Bse3DI GCAATG 2 cut(s) 243, 586
BseBI CCWGG 2 cut(s) 84, 1023
BseDI CCNNGG 7 cut(s) 24, 83, 184, 252, 591, 777, 1021
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 3 cut(s) 417, 500, 817
BseMI GCAATG 2 cut(s) 243, 586
BseMII CTCAG 4 cut(s) 426, 472, 532, 614
BseNI ACTGG 3 cut(s) 192, 767, 822
BseRI GAGGAG 1 cut(s) 648
BseSI GKGCMC 1 cut(s) 199
BseXI GCAGC 2 cut(s) 107, 869
Bsh1236I CGCG 1 cut(s) 525
BshNI GGYRCC 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 199
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 2 cut(s) 276, 318
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 3 cut(s) 417, 500, 817
BsmAI GTCTC 1 cut(s) 981
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 489
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 199, 1021
Bsp143I GATC 3 cut(s) 133, 296, 773
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 5 cut(s) 100, 273, 337, 432, 469
BspCNI CTCAG 4 cut(s) 425, 471, 531, 615
BspFNI CGCG 1 cut(s) 525
BspHI TCATGA 1 cut(s) 568
BspLI GGNNCC 4 cut(s) 190, 562, 1018, 1062
BspOI GCTAGC 1 cut(s) 372
BspPI GGATC 2 cut(s) 304, 768
BspT107I GGYRCC 1 cut(s) 188
BsrDI GCAATG 2 cut(s) 243, 586
BsrFI RCCGGY 2 cut(s) 275, 317
BsrI ACTGG 3 cut(s) 192, 767, 822
BssAI RCCGGY 2 cut(s) 275, 317
BssECI CCNNGG 7 cut(s) 24, 83, 184, 252, 591, 777, 1021
BssMI GATC 3 cut(s) 133, 296, 773
BssT1I CCWWGG 5 cut(s) 24, 184, 252, 591, 777
Bst2UI CCWGG 2 cut(s) 84, 1023
Bst4CI ACNGT 2 cut(s) 482, 513
Bst6I CTCTTC 2 cut(s) 68, 84
BstBAI YACGTR 1 cut(s) 998
BstC8I GCNNGC 6 cut(s) 100, 144, 277, 319, 370, 897
BstDEI CTNAG 6 cut(s) 207, 412, 458, 518, 533, 623
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 1 cut(s) 525
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 3 cut(s) 289, 425, 525
BstKTI GATC 3 cut(s) 136, 299, 776
BstMAI GTCTC 1 cut(s) 981
BstMBI GATC 3 cut(s) 133, 296, 773
BstMWI GCNNNNNNNGC 4 cut(s) 429, 438, 475, 863
BstNI CCWGG 2 cut(s) 84, 1023
BstSCI CCNGG 2 cut(s) 82, 1021
BstSLI GKGCMC 1 cut(s) 199
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 2 cut(s) 107, 869
BstX2I RGATCY 1 cut(s) 296
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 1 cut(s) 296
Bsu36I CCTNAGG 1 cut(s) 412
BsuI GTATCC 1 cut(s) 1037
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 3 cut(s) 18, 199, 774
Cac8I GCNNGC 6 cut(s) 100, 144, 277, 319, 370, 897
CaiI CAGNNNCTG 1 cut(s) 817
CciI TCATGA 1 cut(s) 568
CfoI GCGC 3 cut(s) 289, 425, 525
Cfr10I RCCGGY 2 cut(s) 275, 317
Csp6I GTAC 2 cut(s) 449, 1001
CviAII CATG 4 cut(s) 25, 48, 215, 569
CviQI GTAC 2 cut(s) 449, 1001
DdeI CTNAG 6 cut(s) 207, 412, 458, 518, 533, 623
DpnI GATC 3 cut(s) 135, 298, 775
DpnII GATC 3 cut(s) 133, 296, 773
Eam1104I CTCTTC 2 cut(s) 68, 84
EarI CTCTTC 2 cut(s) 68, 84
Eco130I CCWWGG 5 cut(s) 24, 184, 252, 591, 777
Eco24I GRGCYC 1 cut(s) 1021
Eco57I CTGAAG 1 cut(s) 882
Eco72I CACGTG 1 cut(s) 998
Eco81I CCTNAGG 1 cut(s) 412
Eco88I CYCGRG 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 82, 1021
EcoT14I CCWWGG 5 cut(s) 24, 184, 252, 591, 777
EcoT22I ATGCAT 2 cut(s) 491, 742
EcoT38I GRGCYC 1 cut(s) 1021
ErhI CCWWGG 5 cut(s) 24, 184, 252, 591, 777
FaeI CATG 4 cut(s) 28, 51, 218, 572
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FaqI GGGAC 1 cut(s) 100
FatI CATG 4 cut(s) 24, 47, 214, 568
FauI CCCGC 1 cut(s) 107
FblI GTMKAC 2 cut(s) 398, 648
Fnu4HI GCNGC 3 cut(s) 96, 470, 858
FriOI GRGCYC 1 cut(s) 1021
Fsp4HI GCNGC 3 cut(s) 96, 470, 858
FspBI CTAG 3 cut(s) 369, 687, 962
GlaI GCGC 3 cut(s) 288, 424, 524
GluI GCNGC 3 cut(s) 96, 470, 858
GsuI CTGGAG 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 426
HapII CCGG 2 cut(s) 276, 318
HhaI GCGC 3 cut(s) 289, 425, 525
Hin1II CATG 4 cut(s) 28, 51, 218, 572
Hin6I GCGC 3 cut(s) 287, 423, 523
HinP1I GCGC 3 cut(s) 287, 423, 523
HinfI GANTC 3 cut(s) 105, 220, 907
HpaII CCGG 2 cut(s) 276, 318
HphI GGTGA 2 cut(s) 157, 958
Hpy166II GTNNAC 7 cut(s) 197, 399, 451, 649, 921, 1001, 1085
Hpy188I TCNGA 3 cut(s) 461, 624, 1099
Hpy188III TCNNGA 3 cut(s) 311, 569, 687
Hpy8I GTNNAC 7 cut(s) 197, 399, 451, 649, 921, 1001, 1085
Hpy99I CGWCG 1 cut(s) 530
HpyAV CCTTC 2 cut(s) 742, 805
HpyCH4III ACNGT 2 cut(s) 482, 513
HpyCH4IV ACGT 2 cut(s) 447, 997
HpyF10VI GCNNNNNNNGC 4 cut(s) 429, 438, 475, 863
HpyF3I CTNAG 6 cut(s) 207, 412, 458, 518, 533, 623
HpySE526I ACGT 2 cut(s) 447, 997
Hsp92II CATG 4 cut(s) 28, 51, 218, 572
HspAI GCGC 3 cut(s) 287, 423, 523
KroI GCCGGC 2 cut(s) 275, 317
KroNI GCCGGC 2 cut(s) 277, 319
Kzo9I GATC 3 cut(s) 133, 296, 773
LmnI GCTCC 4 cut(s) 420, 560, 635, 1016
Lsp1109I GCAGC 2 cut(s) 107, 869
LweI GCATC 3 cut(s) 304, 316, 955
MaeI CTAG 3 cut(s) 369, 687, 962
MaeII ACGT 2 cut(s) 447, 997
MaeIII GTNAC 3 cut(s) 384, 946, 993
MalI GATC 3 cut(s) 135, 298, 775
MboI GATC 3 cut(s) 133, 296, 773
MboII GAAGA 4 cut(s) 55, 71, 710, 967
MflI RGATCY 1 cut(s) 296
MhlI GDGCHC 2 cut(s) 199, 1021
MluCI AATT 6 cut(s) 6, 32, 652, 660, 667, 941
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 398
MnlI CCTC 8 cut(s) 22, 48, 165, 178, 421, 422, 618, 626
Mph1103I ATGCAT 2 cut(s) 491, 742
MroNI GCCGGC 2 cut(s) 275, 317
MseI TTAA 1 cut(s) 734
MslI CAYNNNNRTG 2 cut(s) 23, 312
MspA1I CMGCKG 3 cut(s) 860, 1040, 1067
MspI CCGG 2 cut(s) 276, 318
MspR9I CCNGG 2 cut(s) 84, 1023
Mva1269I GAATGC 1 cut(s) 489
MvaI CCWGG 2 cut(s) 84, 1023
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 4 cut(s) 429, 438, 475, 863
NaeI GCCGGC 2 cut(s) 277, 319
NcoI CCATGG 1 cut(s) 24
NdeII GATC 3 cut(s) 133, 296, 773
NgoMIV GCCGGC 2 cut(s) 275, 317
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 4 cut(s) 28, 51, 218, 572
NlaIV GGNNCC 4 cut(s) 190, 562, 1018, 1062
NmuCI GTSAC 3 cut(s) 384, 946, 993
NsiI ATGCAT 2 cut(s) 491, 742
PaeR7I CTCGAG 1 cut(s) 169
PagI TCATGA 1 cut(s) 568
PctI GAATGC 1 cut(s) 489
PdiI GCCGGC 2 cut(s) 277, 319
PfeI GAWTC 2 cut(s) 220, 907
PflMI CCANNNNNTGG 1 cut(s) 817
PkrI GCNGC 3 cut(s) 97, 471, 859
PleI GAGTC 1 cut(s) 99
PmaCI CACGTG 1 cut(s) 998
PmlI CACGTG 1 cut(s) 998
PpsI GAGTC 1 cut(s) 99
Ppu21I YACGTR 1 cut(s) 998
Psp6I CCWGG 2 cut(s) 82, 1021
PspCI CACGTG 1 cut(s) 998
PspGI CCWGG 2 cut(s) 82, 1021
PspN4I GGNNCC 4 cut(s) 190, 562, 1018, 1062
PspXI VCTCGAGB 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 817
PsuI RGATCY 1 cut(s) 296
PvuII CAGCTG 3 cut(s) 860, 1040, 1067
RsaI GTAC 2 cut(s) 450, 1002
RsaNI GTAC 2 cut(s) 449, 1001
RseI CAYNNNNRTG 2 cut(s) 23, 312
SaqAI TTAA 1 cut(s) 734
SatI GCNGC 3 cut(s) 96, 470, 858
Sau3AI GATC 3 cut(s) 133, 296, 773
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 2 cut(s) 84, 1023
SduI GDGCHC 2 cut(s) 199, 1021
SfaNI GCATC 3 cut(s) 304, 316, 955
Sfr274I CTCGAG 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 2 cut(s) 23, 312
SmlI CTYRAG 3 cut(s) 169, 373, 473
SmoI CTYRAG 3 cut(s) 169, 373, 473
Sse9I AATT 6 cut(s) 6, 32, 652, 660, 667, 941
SsiI CCGC 5 cut(s) 100, 273, 337, 432, 469
SspMI CTAG 3 cut(s) 369, 687, 962
StyD4I CCNGG 2 cut(s) 82, 1021
StyI CCWWGG 5 cut(s) 24, 184, 252, 591, 777
TaaI ACNGT 2 cut(s) 482, 513
TaiI ACGT 2 cut(s) 450, 1000
TaqI TCGA 1 cut(s) 170
TaqII GACCGA 1 cut(s) 861
TasI AATT 6 cut(s) 6, 32, 652, 660, 667, 941
TatI WGTACW 1 cut(s) 1000
TauI GCSGC 1 cut(s) 472
TfiI GAWTC 2 cut(s) 220, 907
Tru1I TTAA 1 cut(s) 734
Tru9I TTAA 1 cut(s) 734
TscAI CASTG 3 cut(s) 25, 199, 774
TseFI GTSAC 3 cut(s) 384, 946, 993
TseI GCWGC 2 cut(s) 95, 857
Tsp45I GTSAC 3 cut(s) 384, 946, 993
TspDTI ATGAA 4 cut(s) 278, 557, 597, 711
TspGWI ACGGA 2 cut(s) 543, 565
TspRI CASTG 3 cut(s) 25, 199, 774
Van91I CCANNNNNTGG 1 cut(s) 817
VneI GTGCAC 1 cut(s) 195
XapI RAATTY 1 cut(s) 32
XbaI TCTAGA 1 cut(s) 686
XhoI CTCGAG 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 398, 648
XspI CTAG 3 cut(s) 369, 687, 962
Zsp2I ATGCAT 2 cut(s) 491, 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.