Rw6G002100

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000427
Physical Location & Seq
Reverse (-)
3304612 .. 3306898
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002100.1

Sequence Viewer

Length: 1470 bp
ATGGCTACAAAACCACCATCATGCTTTTCTCTCTTCTCAGTATTGGTGTTGGTTCTTCTAGCCTTAACATCATTTGGGTCCCAAAGTTTCACTGTCCATGGGTTGTCAATTAGAGAAGCTACAATCTACGATCTTCAGCTGGCTTTCAAACAGAACCAACTAACTTCCAGACAAGTAGTTCAGTTCTACCTTCAAGAAATCCGCAGGCTCAACCCGGTTCTTAACGGAGTCATAGAGGTGAACCCGGATGCACTTTACCAAGCTGACAAGGCTGACTATCAGCGAAAGGCTAAGGCCCCTGGTTATTATTCCGGCTTGCATGGCATTCCTGTTTTGTTGAAGGATAACATTGGAACCAGAGATAAGCTGAACACTACTGCTGGCTCTTTTGCGCTGCTCGGCTCTGTCGTGCCCCGGGATGCTGGCGTGGTGACCAAGTTGAGGAGCGCTGGAGCCATTATCTTGGGGAAGGCTAGCTTGAGTGAGTGGGCTCAGGTCAGGTCTCTTACGTCACCCCCAGGTTTCAGCCCCAGAGGTGGCCAAGGAAAGAACCCTTATGTACTATCTGCAAGTCCATGTGGATCAAGCAGTGGATCATCAATATCAGTTGCAGCAAATTTGGTAGCTGTATCTCTTGGTACTGAGACCGATGGCTCAATACTATGTCCAGCAAGCTTTAACTCAGTAGTCGGCATCAAACCAACCGTTGGACTCACAAGCCGAGCCGGGGTGATCCCAGTTACTCCCAGACAGGACACCATTGGACCTATCTGCAGGACTGTGATAGATGCTGTTTATGTTCTTGATGCGATTGTAGGCTATGACTACAAGGATCAAGCAACCACAGAAGCATCGAAATACATTCCACGAGGTGGGTATAAGCAGTTCCTTGGGACTTATGGGCTCAAGGGAAAGAGACTGGGAATAGTAAGGAATCCCTTCTTCACCTCTGGGAGTGCTGTTTTGGTAGACCATCTGGAAATAGCAAACATTGATACTATCTTGAATTTTAATTTGAGTGGAGAAGCAATAGCAACAGTGGCTGAACTTGTGGTGTCCCCAGTTCGATCTTTGGCAGATATCATAGCCTTCAACTTGAAGTTTTCTGGTGTGGAAATGATCAAGGAATTTGGCCAAGACATTTTTCTTGCAGCACAAGCCACAAATGGGATAGGCAACAATGAGAAGGCAGCATTGTTAAATTTAGCAAAGCTGACAAAAGATGGATTTGAGAAACTAATGACATACAACAGGCTAGATGCATTGGTGACTCCCGGTGCAGATATTGCTCCTGTACTTGCAATTGGGGGATTCCCAGGCATTAGTGTCCCTGCTGGATATGACAAGAAGGGTGTGCCTTTCGGCATAACTTTTGGGGGTCTAAAGGGATCTGAACCAAAGCTTATACAGATTGCCTACGGTTTCGAGCAAGCTACTGAGATTAGAAAGCCTCCCACATTTCTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

489

Amino Acids

51.81

Weight (kDa)

9.17

Isoelectric Point (pI)

31.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 59 - 314 1.1e-66 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 707, 872
AccI GTMKAC 1 cut(s) 969
AciI CCGC 1 cut(s) 202
AclWI GGATC 5 cut(s) 589, 601, 727, 840, 1396
AcoI YGGCCR 2 cut(s) 538, 1132
AcsI RAATTY 4 cut(s) 616, 1006, 1127, 1201
AcuI CTGAAG 1 cut(s) 119
AdeI CACNNNGTG 1 cut(s) 872
AfaI GTAC 3 cut(s) 561, 640, 1296
AfeI AGCGCT 1 cut(s) 448
AfiI CCNNNNNNNGG 6 cut(s) 441, 536, 707, 727, 872, 1167
AgsI TTSAA 6 cut(s) 148, 194, 340, 1006, 1093, 1099
AjnI CCWGG 3 cut(s) 298, 517, 1315
AjuI GAANNNNNNNTTGG 2 cut(s) 1128, 1160
Alw26I GTCTC 3 cut(s) 507, 638, 910
AlwI GGATC 5 cut(s) 589, 601, 727, 840, 1396
AlwNI CAGNNNCTG 1 cut(s) 1043
Ama87I CYCGRG 1 cut(s) 414
Aor51HI AGCGCT 1 cut(s) 448
AoxI GGCC 3 cut(s) 294, 538, 1132
ApeKI GCWGC 4 cut(s) 394, 611, 1151, 1190
ApoI RAATTY 4 cut(s) 616, 1006, 1127, 1201
Asp700I GAANNNNTTC 1 cut(s) 938
AspLEI GCGC 2 cut(s) 394, 449
AspS9I GGNCC 3 cut(s) 78, 295, 764
AsuC2I CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1275
AsuHPI GGTGA 6 cut(s) 250, 442, 504, 742, 937, 1279
AsuNHI GCTAGC 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 2 cut(s) 78, 764
BaeGI GKGCMC 1 cut(s) 414
BalI TGGCCA 2 cut(s) 540, 1134
BanII GRGCYC 2 cut(s) 493, 906
BauI CACGAG 1 cut(s) 867
BbvI GCAGC 4 cut(s) 381, 623, 1163, 1202
BccI CCATC 4 cut(s) 25, 644, 981, 1217
BciT130I CCWGG 3 cut(s) 300, 519, 1317
BclI TGATCA 1 cut(s) 1119
BcnI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1275
BcoDI GTCTC 3 cut(s) 507, 638, 910
BfaI CTAG 3 cut(s) 59, 474, 1256
BfmI CTRYAG 1 cut(s) 772
BfoI RGCGCY 1 cut(s) 450
BisI GCNGC 4 cut(s) 395, 612, 1152, 1191
BlsI GCNGC 4 cut(s) 396, 613, 1153, 1192
Bme1390I CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1275, 1317
Bme18I GGWCC 2 cut(s) 78, 764
BmeT110I CYCGRG 1 cut(s) 414
BmgT120I GGNCC 3 cut(s) 78, 295, 764
BmiI GGNNCC 5 cut(s) 79, 80, 297, 355, 454
BmrFI CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1275, 1317
BmrI ACTGGG 3 cut(s) 731, 929, 1055
BmsI GCATC 7 cut(s) 238, 409, 702, 778, 796, 860, 1249
BmtI GCTAGC 1 cut(s) 477
BmuI ACTGGG 3 cut(s) 731, 929, 1055
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 2 cut(s) 291, 492
BpuEI CTTGAG 2 cut(s) 499, 890
BpuMI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1275
BsaI GGTCTC 2 cut(s) 507, 638
BsaJI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 889, 1315
Bsc4I CCNNNNNNNGG 6 cut(s) 441, 536, 707, 727, 872, 1167
Bse1I ACTGG 3 cut(s) 737, 924, 1061
BseBI CCWGG 3 cut(s) 300, 519, 1317
BseDI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 889, 1315
BseGI GGATG 2 cut(s) 253, 424
BseLI CCNNNNNNNGG 6 cut(s) 441, 536, 707, 727, 872, 1167
BseMII CTCAG 5 cut(s) 51, 506, 633, 696, 1428
BseNI ACTGG 3 cut(s) 737, 924, 1061
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 414
BseXI GCAGC 4 cut(s) 381, 623, 1163, 1202
BsgI GTGCAG 1 cut(s) 1299
BshFI GGCC 3 cut(s) 296, 540, 1134
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 6 cut(s) 215, 245, 312, 415, 726, 1275
BslFI GGGAC 4 cut(s) 64, 907, 1042, 1313
BslI CCNNNNNNNGG 6 cut(s) 441, 536, 707, 727, 872, 1167
BsmAI GTCTC 3 cut(s) 507, 638, 910
BsmFI GGGAC 4 cut(s) 64, 907, 1042, 1313
BsmI GAATGC 1 cut(s) 324
BsnI GGCC 3 cut(s) 296, 540, 1134
Bso31I GGTCTC 2 cut(s) 507, 638
BsoBI CYCGRG 1 cut(s) 414
Bsp1286I GDGCHC 3 cut(s) 414, 493, 906
Bsp143I GATC 8 cut(s) 130, 581, 593, 732, 832, 1067, 1119, 1388
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 1 cut(s) 202
BspANI GGCC 3 cut(s) 296, 540, 1134
BspCNI CTCAG 5 cut(s) 50, 505, 634, 695, 1429
BspLI GGNNCC 5 cut(s) 79, 80, 297, 355, 454
BspMAI CTGCAG 1 cut(s) 776
BspOI GCTAGC 1 cut(s) 477
BspPI GGATC 5 cut(s) 589, 601, 727, 840, 1396
BspTNI GGTCTC 2 cut(s) 507, 638
BsrI ACTGG 3 cut(s) 737, 924, 1061
BssECI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 889, 1315
BssMI GATC 8 cut(s) 130, 581, 593, 732, 832, 1067, 1119, 1388
BssSI CACGAG 1 cut(s) 867
BssT1I CCWWGG 3 cut(s) 97, 541, 889
Bst2BI CACGAG 1 cut(s) 867
Bst2UI CCWGG 3 cut(s) 300, 519, 1317
Bst4CI ACNGT 5 cut(s) 94, 706, 781, 1039, 1421
Bst6I CTCTTC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 1286
BstC8I GCNNGC 8 cut(s) 141, 206, 317, 382, 424, 475, 673, 1431
BstDEI CTNAG 6 cut(s) 37, 291, 492, 642, 682, 1437
BstDSI CCRYGG 1 cut(s) 97
BstEII GGTNACC 1 cut(s) 430
BstF5I GGATG 2 cut(s) 253, 424
BstH2I RGCGCY 1 cut(s) 450
BstHHI GCGC 2 cut(s) 394, 449
BstKTI GATC 8 cut(s) 133, 584, 596, 735, 835, 1070, 1122, 1391
BstMAI GTCTC 3 cut(s) 507, 638, 910
BstMBI GATC 8 cut(s) 130, 581, 593, 732, 832, 1067, 1119, 1388
BstMWI GCNNNNNNNGC 5 cut(s) 269, 321, 1040, 1157, 1286
BstNI CCWGG 3 cut(s) 300, 519, 1317
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 9 cut(s) 213, 243, 298, 413, 414, 517, 725, 1273, 1315
BstSFI CTRYAG 1 cut(s) 772
BstSLI GKGCMC 1 cut(s) 414
BstV1I GCAGC 4 cut(s) 381, 623, 1163, 1202
BstX2I RGATCY 1 cut(s) 1388
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 1 cut(s) 1388
BsuRI GGCC 3 cut(s) 296, 540, 1134
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 2 cut(s) 253, 424
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 3 cut(s) 90, 595, 1044
Cac8I GCNNGC 8 cut(s) 141, 206, 317, 382, 424, 475, 673, 1431
CaiI CAGNNNCTG 1 cut(s) 1043
CfoI GCGC 2 cut(s) 394, 449
Cfr13I GGNCC 3 cut(s) 78, 295, 764
Cfr9I CCCGGG 1 cut(s) 414
Csp6I GTAC 3 cut(s) 560, 639, 1295
CviAII CATG 4 cut(s) 21, 98, 320, 576
CviQI GTAC 3 cut(s) 560, 639, 1295
DdeI CTNAG 6 cut(s) 37, 291, 492, 642, 682, 1437
DpnI GATC 8 cut(s) 132, 583, 595, 734, 834, 1069, 1121, 1390
DpnII GATC 8 cut(s) 130, 581, 593, 732, 832, 1067, 1119, 1388
DraIII CACNNNGTG 1 cut(s) 872
EaeI YGGCCR 2 cut(s) 538, 1132
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 3 cut(s) 97, 541, 889
Eco24I GRGCYC 2 cut(s) 493, 906
Eco31I GGTCTC 2 cut(s) 507, 638
Eco32I GATATC 1 cut(s) 1081
Eco47I GGWCC 2 cut(s) 78, 764
Eco47III AGCGCT 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 119
Eco88I CYCGRG 1 cut(s) 414
Eco91I GGTNACC 1 cut(s) 430
EcoO109I RGGNCCY 2 cut(s) 78, 295
EcoO65I GGTNACC 1 cut(s) 430
EcoRII CCWGG 3 cut(s) 298, 517, 1315
EcoRV GATATC 1 cut(s) 1081
EcoT14I CCWWGG 3 cut(s) 97, 541, 889
EcoT22I ATGCAT 1 cut(s) 1264
EcoT38I GRGCYC 2 cut(s) 493, 906
ErhI CCWWGG 3 cut(s) 97, 541, 889
FaeI CATG 4 cut(s) 24, 101, 323, 579
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 4 cut(s) 64, 907, 1042, 1313
FatI CATG 4 cut(s) 20, 97, 319, 575
FbaI TGATCA 1 cut(s) 1119
FblI GTMKAC 1 cut(s) 969
Fnu4HI GCNGC 4 cut(s) 395, 612, 1152, 1191
FokI GGATG 2 cut(s) 260, 431
FriOI GRGCYC 2 cut(s) 493, 906
Fsp4HI GCNGC 4 cut(s) 395, 612, 1152, 1191
FspBI CTAG 3 cut(s) 59, 474, 1256
GlaI GCGC 2 cut(s) 393, 448
GluI GCNGC 4 cut(s) 395, 612, 1152, 1191
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 450
HaeIII GGCC 3 cut(s) 296, 540, 1134
HapII CCGG 6 cut(s) 215, 245, 312, 415, 726, 1275
HhaI GCGC 2 cut(s) 394, 449
Hin1II CATG 4 cut(s) 24, 101, 323, 579
Hin6I GCGC 2 cut(s) 392, 447
HinP1I GCGC 2 cut(s) 392, 447
HindIII AAGCTT 2 cut(s) 673, 1400
HinfI GANTC 5 cut(s) 228, 711, 934, 1270, 1311
HpaII CCGG 6 cut(s) 215, 245, 312, 415, 726, 1275
HphI GGTGA 6 cut(s) 250, 442, 504, 742, 937, 1279
Hpy166II GTNNAC 2 cut(s) 241, 970
Hpy188I TCNGA 1 cut(s) 1393
Hpy188III TCNNGA 5 cut(s) 168, 194, 803, 977, 1003
Hpy8I GTNNAC 2 cut(s) 241, 970
HpyAV CCTTC 7 cut(s) 200, 334, 463, 949, 1099, 1180, 1342
HpyCH4III ACNGT 5 cut(s) 94, 706, 781, 1039, 1421
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 9 cut(s) 251, 319, 569, 611, 774, 1151, 1262, 1280, 1301
HpyF10VI GCNNNNNNNGC 5 cut(s) 269, 321, 1040, 1157, 1286
HpyF3I CTNAG 6 cut(s) 37, 291, 492, 642, 682, 1437
HpySE526I ACGT 1 cut(s) 509
Hsp92II CATG 4 cut(s) 24, 101, 323, 579
HspAI GCGC 2 cut(s) 392, 447
KflI GGGWCCC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 1119
Kzo9I GATC 8 cut(s) 130, 581, 593, 732, 832, 1067, 1119, 1388
LmnI GCTCC 3 cut(s) 444, 452, 1294
Lsp1109I GCAGC 4 cut(s) 381, 623, 1163, 1202
LweI GCATC 7 cut(s) 238, 409, 702, 778, 796, 860, 1249
MaeI CTAG 3 cut(s) 59, 474, 1256
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 4 cut(s) 430, 510, 739, 1267
MalI GATC 8 cut(s) 132, 583, 595, 734, 834, 1069, 1121, 1390
MboI GATC 8 cut(s) 130, 581, 593, 732, 832, 1067, 1119, 1388
MboII GAAGA 4 cut(s) 25, 47, 125, 934
MfeI CAATTG 1 cut(s) 1302
MflI RGATCY 1 cut(s) 1388
MhlI GDGCHC 3 cut(s) 414, 493, 906
MlsI TGGCCA 2 cut(s) 540, 1134
MluCI AATT 7 cut(s) 108, 616, 1006, 1012, 1127, 1201, 1302
MluNI TGGCCA 2 cut(s) 540, 1134
MlyI GAGTC 3 cut(s) 237, 705, 1264
MmeI TCCRAC 1 cut(s) 688
MnlI CCTC 6 cut(s) 229, 435, 527, 863, 958, 1461
Mox20I TGGCCA 2 cut(s) 540, 1134
Mph1103I ATGCAT 1 cut(s) 1264
MroXI GAANNNNTTC 1 cut(s) 938
MscI TGGCCA 2 cut(s) 540, 1134
MseI TTAA 5 cut(s) 65, 222, 678, 1011, 1199
MslI CAYNNNNRTG 2 cut(s) 19, 236
Msp20I TGGCCA 2 cut(s) 540, 1134
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 6 cut(s) 215, 245, 312, 415, 726, 1275
MspR9I CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1275, 1317
MunI CAATTG 1 cut(s) 1302
Mva1269I GAATGC 1 cut(s) 324
MvaI CCWGG 3 cut(s) 300, 519, 1317
MwoI GCNNNNNNNGC 5 cut(s) 269, 321, 1040, 1157, 1286
NciI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1275
NcoI CCATGG 1 cut(s) 97
NdeII GATC 8 cut(s) 130, 581, 593, 732, 832, 1067, 1119, 1388
NheI GCTAGC 1 cut(s) 473
NlaIII CATG 4 cut(s) 24, 101, 323, 579
NlaIV GGNNCC 5 cut(s) 79, 80, 297, 355, 454
NmeAIII GCCGAG 2 cut(s) 378, 746
NmuCI GTSAC 3 cut(s) 430, 510, 1267
NsiI ATGCAT 1 cut(s) 1264
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PctI GAATGC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 938
PfeI GAWTC 2 cut(s) 934, 1311
PflMI CCANNNNNTGG 2 cut(s) 707, 872
PkrI GCNGC 4 cut(s) 396, 613, 1153, 1192
PleI GAGTC 3 cut(s) 236, 705, 1264
PpsI GAGTC 3 cut(s) 236, 705, 1264
PpuMI RGGWCCY 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 78
Psp6I CCWGG 3 cut(s) 298, 517, 1315
PspEI GGTNACC 1 cut(s) 430
PspGI CCWGG 3 cut(s) 298, 517, 1315
PspN4I GGNNCC 5 cut(s) 79, 80, 297, 355, 454
PspPI GGNCC 3 cut(s) 78, 295, 764
PspPPI RGGWCCY 1 cut(s) 78
PsrI GAACNNNNNNTAC 2 cut(s) 869, 901
PstI CTGCAG 1 cut(s) 776
PstNI CAGNNNCTG 1 cut(s) 1043
PsuI RGATCY 1 cut(s) 1388
PvuII CAGCTG 1 cut(s) 139
RsaI GTAC 3 cut(s) 561, 640, 1296
RsaNI GTAC 3 cut(s) 560, 639, 1295
RseI CAYNNNNRTG 2 cut(s) 19, 236
SaqAI TTAA 5 cut(s) 65, 222, 678, 1011, 1199
SatI GCNGC 4 cut(s) 395, 612, 1152, 1191
Sau3AI GATC 8 cut(s) 130, 581, 593, 732, 832, 1067, 1119, 1388
Sau96I GGNCC 3 cut(s) 78, 295, 764
SchI GAGTC 3 cut(s) 237, 705, 1264
ScrFI CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1275, 1317
SduI GDGCHC 3 cut(s) 414, 493, 906
SfaNI GCATC 7 cut(s) 238, 409, 702, 778, 796, 860, 1249
SfcI CTRYAG 1 cut(s) 772
SinI GGWCC 2 cut(s) 78, 764
SmaI CCCGGG 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 19, 236
SmlI CTYRAG 2 cut(s) 478, 905
SmoI CTYRAG 2 cut(s) 478, 905
Sse9I AATT 7 cut(s) 108, 616, 1006, 1012, 1127, 1201, 1302
SsiI CCGC 1 cut(s) 202
SspMI CTAG 3 cut(s) 59, 474, 1256
StyD4I CCNGG 9 cut(s) 213, 243, 298, 413, 414, 517, 725, 1273, 1315
StyI CCWWGG 3 cut(s) 97, 541, 889
TaaI ACNGT 5 cut(s) 94, 706, 781, 1039, 1421
TaiI ACGT 1 cut(s) 512
TaqI TCGA 3 cut(s) 854, 1066, 1425
TaqII GACCGA 1 cut(s) 662
TasI AATT 7 cut(s) 108, 616, 1006, 1012, 1127, 1201, 1302
TatI WGTACW 2 cut(s) 559, 1294
TfiI GAWTC 2 cut(s) 934, 1311
Tru1I TTAA 5 cut(s) 65, 222, 678, 1011, 1199
Tru9I TTAA 5 cut(s) 65, 222, 678, 1011, 1199
TscAI CASTG 3 cut(s) 97, 595, 1044
TseFI GTSAC 3 cut(s) 430, 510, 1267
TseI GCWGC 4 cut(s) 394, 611, 1151, 1190
Tsp45I GTSAC 3 cut(s) 430, 510, 1267
TspGWI ACGGA 1 cut(s) 240
TspMI CCCGGG 1 cut(s) 414
TspRI CASTG 3 cut(s) 97, 595, 1044
Van91I CCANNNNNTGG 2 cut(s) 707, 872
VpaK11BI GGWCC 2 cut(s) 78, 764
XapI RAATTY 4 cut(s) 616, 1006, 1127, 1201
XmaI CCCGGG 1 cut(s) 414
XmiI GTMKAC 1 cut(s) 969
XmnI GAANNNNTTC 1 cut(s) 938
XspI CTAG 3 cut(s) 59, 474, 1256
Zsp2I ATGCAT 1 cut(s) 1264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.