FvH4_2g01720

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000427
Physical Location & Seq
Reverse (-)
1513190 .. 1516764
3575 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01720.t1

Sequence Viewer

Length: 1611 bp
ATGGCTACCAATTCATGTTCTCTCTTCTGGGTTCTGCTTCTAATTCTTCTCCAAATCACCCTATCAAATGGGTCTGAATCCACAACACCCAGAAGCCTAATGGAAGAAGCCACAGTCGATGATCTCCAACTCGCTTTCAAGCAAAAGCTTCTTACTTCAAGGCAAGTCGTGCAGTTCTACCTGGACCAAATCCAAAAACTCAACCAAGACCTCAGAGGAGTCTTAGAGATAAACCCAGATGCTTTATCTCAAGCTGACATAGCAGACAAAGAGCGCAGTGAATTTGAATCCAGTTTTCGTACACTGCCTAAGCTTCATGGGATTCCTGTTTTGCTGAAAGATAATATAGCAACCAAGGACAAGCTTAACACGACTGCTGGGTCTTTTGCTCTTTTGGGTTCTGTAGTGCCTAGAGATGCTGGGGTTGTCTCAAAGTTGAGGATCTCTGGGGCTATCATTTTGGGGAAGGCTACTTTGAGTGAATGGGCTTATATCAGATCATCTACAGCACCTTATGGTTGGAGTGCCCGAGGTGGTCAAGGAGTGAATCCTTATAATTCATCACTAGAAGTTTGTGGATCAAGTAGTGGATCCGCAATATCAGTTTCTGCAAATATGGTATCAGTCGCGCTAGGAACGGAGACCGATAGCTCCATCTTATGTCCAGCCAGTTTTAACTCGGTAGTGGGATTCAAACCAACTGTTGGTCTTACTAGTCGAGCAGGCGTCATCCCAGTCAGTCCAAGACAGGACACAATTGGGCCAATTTGTAGGACTGTAGCAGATGCAGTTCATGTCCTTGATACCATTGTTGGCATTGATAGCAATGACAACACGACAAGTGAAGTATCCCAGTATATTCCAGTGGGCGGCTATGGCCAGTTTCTCAATTCTAGTGGTCTCAAAGGGAAGAGACTGGGGATAGTCAGTCGCATGTTTCTTCCGCTCAAGGATGATGCTTTCTTGAATCAAACTTTTGAGCAGCATTTTACCACACTGAGGGAACAAGGTGCAATTTTGGTTGAGAATCTTGAAATAGCCAACCTTGAAGATATTCGCGATTTTACTTCAAGTGGTGAGTTTGTAGCAATGTTAGCTGAATTCAAGATTGCCTTGAATGCTTACCTGGGTGACTTGGTGAAATCTCCTGTGAGAACCTTAGGCGAGGTTATAGCATTCAACAAGAATAATTCTTATCTGGAAAAGAATGATGATTATGGACAAAATCTTTTAGAAGCAGCTGAGGAGACTAAAGGAATGGGGGAGAAAGAGCTGGAGGCATTGTCAAATATAGCGACACTATCTAGAGATGGATTTGAGAAGTTGATGGCAGATAAGAACTTAGATGCATTGGTGACTTATGCTAATACCGCTTCTTCTATCCTCGCAATTGGTGGTTTCCCGGGCATTGTTGTGCCTGCAGGATATCATGATGCTGATAAGTACCCTTTTGGAATTTGCTTTGGGGGACTGAAGGGTTCGGAGCCAAAACTGATTGAGATTTCCTATGCCTTCGAGCAAGCAACTATGATCAGGAAGCCTCCAACTGTTGATTCCCACTACCAAAGCATCCCTACTGCCACAAGAAGCTCCAACCTAGCTACTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

537

Amino Acids

57.72

Weight (kDa)

5.06

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 56 - 453 7.1e-70 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
AasI GACNNNNNNGTC 1 cut(s) 379
AccB7I CCANNNNNTGG 1 cut(s) 704
AccBSI CCGCTC 1 cut(s) 946
AccII CGCG 2 cut(s) 629, 1059
AciI CCGC 4 cut(s) 594, 870, 944, 1371
AclWI GGATC 4 cut(s) 449, 585, 586, 598
AcoI YGGCCR 1 cut(s) 877
AcsI RAATTY 3 cut(s) 281, 1100, 1455
AcuI CTGAAG 1 cut(s) 1493
AcyI GRCGYC 1 cut(s) 726
AfaI GTAC 2 cut(s) 301, 1445
AfiI CCNNNNNNNGG 3 cut(s) 704, 869, 999
AhlI ACTAGT 1 cut(s) 713
AjnI CCWGG 2 cut(s) 180, 1125
AjuI GAANNNNNNNTTGG 2 cut(s) 1537, 1569
Alw26I GTCTC 5 cut(s) 433, 635, 905, 907, 1241
AlwI GGATC 4 cut(s) 449, 585, 586, 598
AlwNI CAGNNNCTG 1 cut(s) 608
Ama87I CYCGRG 2 cut(s) 528, 1402
AoxI GGCC 2 cut(s) 761, 877
ApeKI GCWGC 2 cut(s) 982, 1238
ApoI RAATTY 3 cut(s) 281, 1100, 1455
Asp700I GAANNNNTTC 1 cut(s) 1053
AspLEI GCGC 2 cut(s) 276, 631
AspS9I GGNCC 2 cut(s) 184, 761
AsuC2I CCSGG 2 cut(s) 1403, 1404
AsuHPI GGTGA 5 cut(s) 49, 1088, 1142, 1150, 1366
AvaI CYCGRG 2 cut(s) 528, 1402
AvaII GGWCC 1 cut(s) 184
AxyI CCTNAGG 1 cut(s) 1159
BaeGI GKGCMC 1 cut(s) 529
BaeI ACNNNNGTAYC 2 cut(s) 795, 828
BalI TGGCCA 1 cut(s) 879
BamHI GGATCC 1 cut(s) 590
BbvCI CCTCAGC 1 cut(s) 1242
BbvI GCAGC 2 cut(s) 994, 1250
BccI CCATC 3 cut(s) 662, 1304, 1321
BciT130I CCWGG 2 cut(s) 182, 1127
BciVI GTATCC 1 cut(s) 859
BclI TGATCA 1 cut(s) 1530
BcnI CCSGG 2 cut(s) 1403, 1404
BcoDI GTCTC 5 cut(s) 433, 635, 905, 907, 1241
BcuI ACTAGT 1 cut(s) 713
BfaI CTAG 7 cut(s) 411, 566, 632, 714, 894, 1305, 1598
BfmI CTRYAG 4 cut(s) 402, 504, 777, 1419
BfuI GTATCC 1 cut(s) 859
BisI GCNGC 3 cut(s) 871, 983, 1239
BlsI GCNGC 3 cut(s) 872, 984, 1240
Bme1390I CCNGG 4 cut(s) 182, 1127, 1403, 1404
Bme18I GGWCC 1 cut(s) 184
BmeT110I CYCGRG 2 cut(s) 528, 1402
BmgT120I GGNCC 2 cut(s) 184, 761
BmiI GGNNCC 2 cut(s) 592, 1485
BmrFI CCNGG 4 cut(s) 182, 1127, 1403, 1404
BmrI ACTGGG 3 cut(s) 728, 847, 926
BmsI GCATC 7 cut(s) 229, 406, 775, 946, 1336, 1423, 1578
BmuI ACTGGG 3 cut(s) 728, 847, 926
BpmI CTGGAG 1 cut(s) 1295
Bpu10I CCTNAGC 2 cut(s) 309, 1242
BpuEI CTTGAG 2 cut(s) 234, 932
BpuMI CCSGG 2 cut(s) 1403, 1404
BsaHI GRCGYC 1 cut(s) 726
BsaI GGTCTC 2 cut(s) 635, 905
BsaJI CCNNGG 4 cut(s) 354, 529, 1126, 1402
BsaXI ACNNNNNCTCC 2 cut(s) 1268, 1298
Bsc4I CCNNNNNNNGG 3 cut(s) 704, 869, 999
Bse1I ACTGG 7 cut(s) 291, 669, 734, 853, 863, 880, 921
Bse21I CCTNAGG 1 cut(s) 1159
Bse3DI GCAATG 2 cut(s) 832, 1095
BseBI CCWGG 2 cut(s) 182, 1127
BseDI CCNNGG 4 cut(s) 354, 529, 1126, 1402
BseGI GGATG 3 cut(s) 729, 958, 1569
BseLI CCNNNNNNNGG 3 cut(s) 704, 869, 999
BseMI GCAATG 2 cut(s) 832, 1095
BseMII CTCAG 3 cut(s) 226, 989, 1233
BseNI ACTGG 7 cut(s) 291, 669, 734, 853, 863, 880, 921
BseRI GAGGAG 2 cut(s) 231, 1259
BseSI GKGCMC 1 cut(s) 529
BseXI GCAGC 2 cut(s) 994, 1250
BseYI CCCAGC 2 cut(s) 377, 419
BsgI GTGCAG 1 cut(s) 191
Bsh1236I CGCG 2 cut(s) 629, 1059
BshFI GGCC 2 cut(s) 763, 879
BsiHKCI CYCGRG 2 cut(s) 528, 1402
BsiSI CCGG 1 cut(s) 1403
BslFI GGGAC 1 cut(s) 1482
BslI CCNNNNNNNGG 3 cut(s) 704, 869, 999
BsmAI GTCTC 5 cut(s) 433, 635, 905, 907, 1241
BsmFI GGGAC 1 cut(s) 1482
BsmI GAATGC 2 cut(s) 1123, 1175
BsnI GGCC 2 cut(s) 763, 879
Bso31I GGTCTC 2 cut(s) 635, 905
BsoBI CYCGRG 2 cut(s) 528, 1402
Bsp1286I GDGCHC 1 cut(s) 529
Bsp143I GATC 6 cut(s) 121, 441, 497, 578, 590, 1530
Bsp68I TCGCGA 1 cut(s) 1059
BspACI CCGC 4 cut(s) 594, 870, 944, 1371
BspANI GGCC 2 cut(s) 763, 879
BspCNI CTCAG 3 cut(s) 225, 990, 1234
BspFNI CGCG 2 cut(s) 629, 1059
BspHI TCATGA 1 cut(s) 1429
BspLI GGNNCC 2 cut(s) 592, 1485
BspMAI CTGCAG 1 cut(s) 1423
BspPI GGATC 4 cut(s) 449, 585, 586, 598
BspTNI GGTCTC 2 cut(s) 635, 905
BsrBI CCGCTC 1 cut(s) 946
BsrDI GCAATG 2 cut(s) 832, 1095
BsrI ACTGG 7 cut(s) 291, 669, 734, 853, 863, 880, 921
BssECI CCNNGG 4 cut(s) 354, 529, 1126, 1402
BssMI GATC 6 cut(s) 121, 441, 497, 578, 590, 1530
BssNI GRCGYC 1 cut(s) 726
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 182, 1127
Bst4CI ACNGT 4 cut(s) 115, 703, 778, 1549
Bst6I CTCTTC 2 cut(s) 29, 905
BstACI GRCGYC 1 cut(s) 726
BstAPI GCANNNNNTGC 1 cut(s) 169
BstC8I GCNNGC 3 cut(s) 724, 1419, 1521
BstDEI CTNAG 7 cut(s) 212, 223, 309, 998, 1159, 1242, 1342
BstF5I GGATG 3 cut(s) 729, 958, 1569
BstFNI CGCG 2 cut(s) 629, 1059
BstHHI GCGC 2 cut(s) 276, 631
BstKTI GATC 6 cut(s) 124, 444, 500, 581, 593, 1533
BstMAI GTCTC 5 cut(s) 433, 635, 905, 907, 1241
BstMBI GATC 6 cut(s) 121, 441, 497, 578, 590, 1530
BstMWI GCNNNNNNNGC 7 cut(s) 169, 260, 822, 876, 1094, 1118, 1370
BstNI CCWGG 2 cut(s) 182, 1127
BstNSI RCATGY 1 cut(s) 937
BstSCI CCNGG 4 cut(s) 180, 1125, 1401, 1402
BstSFI CTRYAG 4 cut(s) 402, 504, 777, 1419
BstSLI GKGCMC 1 cut(s) 529
BstUI CGCG 2 cut(s) 629, 1059
BstV1I GCAGC 2 cut(s) 994, 1250
BstX2I RGATCY 2 cut(s) 441, 590
BstYI RGATCY 2 cut(s) 441, 590
Bsu36I CCTNAGG 1 cut(s) 1159
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 2 cut(s) 763, 879
BtsCI GGATG 3 cut(s) 729, 958, 1569
BtsI GCAGTG 2 cut(s) 283, 302
BtsIMutI CAGTG 4 cut(s) 283, 302, 870, 995
BtuMI TCGCGA 1 cut(s) 1059
Cac8I GCNNGC 3 cut(s) 724, 1419, 1521
CaiI CAGNNNCTG 1 cut(s) 608
CciI TCATGA 1 cut(s) 1429
CfoI GCGC 2 cut(s) 276, 631
Cfr13I GGNCC 2 cut(s) 184, 761
Cfr9I CCCGGG 1 cut(s) 1402
CseI GACGC 1 cut(s) 715
Csp6I GTAC 2 cut(s) 300, 1444
CspCI CAANNNNNGTGG 2 cut(s) 877, 912
CviAII CATG 5 cut(s) 15, 317, 794, 934, 1430
CviQI GTAC 2 cut(s) 300, 1444
DdeI CTNAG 7 cut(s) 212, 223, 309, 998, 1159, 1242, 1342
DpnI GATC 6 cut(s) 123, 443, 499, 580, 592, 1532
DpnII GATC 6 cut(s) 121, 441, 497, 578, 590, 1530
DrdI GACNNNNNNGTC 1 cut(s) 379
DseDI GACNNNNNNGTC 1 cut(s) 379
EaeI YGGCCR 1 cut(s) 877
Eam1104I CTCTTC 2 cut(s) 29, 905
EarI CTCTTC 2 cut(s) 29, 905
Eco130I CCWWGG 1 cut(s) 354
Eco31I GGTCTC 2 cut(s) 635, 905
Eco32I GATATC 1 cut(s) 1427
Eco47I GGWCC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 1493
Eco81I CCTNAGG 1 cut(s) 1159
Eco88I CYCGRG 2 cut(s) 528, 1402
EcoRI GAATTC 1 cut(s) 1100
EcoRII CCWGG 2 cut(s) 180, 1125
EcoRV GATATC 1 cut(s) 1427
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 1351
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 5 cut(s) 18, 320, 797, 937, 1433
FalI AAGNNNNNCTT 2 cut(s) 1097, 1129
FaqI GGGAC 1 cut(s) 1482
FatI CATG 5 cut(s) 14, 316, 793, 933, 1429
FbaI TGATCA 1 cut(s) 1530
Fnu4HI GCNGC 3 cut(s) 871, 983, 1239
FokI GGATG 3 cut(s) 716, 965, 1556
Fsp4HI GCNGC 3 cut(s) 871, 983, 1239
FspBI CTAG 7 cut(s) 411, 566, 632, 714, 894, 1305, 1598
GlaI GCGC 2 cut(s) 275, 630
GluI GCNGC 3 cut(s) 871, 983, 1239
GsaI CCCAGC 2 cut(s) 381, 423
GsuI CTGGAG 1 cut(s) 1295
HaeIII GGCC 2 cut(s) 763, 879
HapII CCGG 1 cut(s) 1403
HgaI GACGC 1 cut(s) 715
HhaI GCGC 2 cut(s) 276, 631
Hin1I GRCGYC 1 cut(s) 726
Hin1II CATG 5 cut(s) 18, 320, 797, 937, 1433
Hin6I GCGC 2 cut(s) 274, 629
HinP1I GCGC 2 cut(s) 274, 629
HindIII AAGCTT 3 cut(s) 146, 311, 362
HinfI GANTC 9 cut(s) 77, 219, 287, 322, 547, 690, 967, 1027, 1553
HpaII CCGG 1 cut(s) 1403
HphI GGTGA 5 cut(s) 49, 1088, 1142, 1150, 1366
Hpy166II GTNNAC 1 cut(s) 302
Hpy188I TCNGA 4 cut(s) 76, 215, 497, 1483
Hpy188III TCNNGA 8 cut(s) 964, 1031, 1058, 1105, 1199, 1305, 1430, 1534
Hpy8I GTNNAC 1 cut(s) 302
HpyAV CCTTC 3 cut(s) 460, 1468, 1522
HpyCH4III ACNGT 4 cut(s) 115, 703, 778, 1549
HpyCH4V TGCA 6 cut(s) 172, 611, 788, 1013, 1349, 1421
HpyF10VI GCNNNNNNNGC 7 cut(s) 169, 260, 822, 876, 1094, 1118, 1370
HpyF3I CTNAG 7 cut(s) 212, 223, 309, 998, 1159, 1242, 1342
Hsp92I GRCGYC 1 cut(s) 726
Hsp92II CATG 5 cut(s) 18, 320, 797, 937, 1433
HspAI GCGC 2 cut(s) 274, 629
Ksp22I TGATCA 1 cut(s) 1530
Kzo9I GATC 6 cut(s) 121, 441, 497, 578, 590, 1530
LmnI GCTCC 3 cut(s) 656, 1483, 1595
Lsp1109I GCAGC 2 cut(s) 994, 1250
LweI GCATC 7 cut(s) 229, 406, 775, 946, 1336, 1423, 1578
MaeI CTAG 7 cut(s) 411, 566, 632, 714, 894, 1305, 1598
MaeIII GTNAC 2 cut(s) 1130, 1354
MalI GATC 6 cut(s) 123, 443, 499, 580, 592, 1532
MbiI CCGCTC 1 cut(s) 946
MboI GATC 6 cut(s) 121, 441, 497, 578, 590, 1530
MboII GAAGA 7 cut(s) 16, 38, 116, 922, 932, 1061, 1368
MfeI CAATTG 2 cut(s) 756, 1389
MflI RGATCY 2 cut(s) 441, 590
MhlI GDGCHC 1 cut(s) 529
MlsI TGGCCA 1 cut(s) 879
MluNI TGGCCA 1 cut(s) 879
MlyI GAGTC 1 cut(s) 228
MmeI TCCRAC 3 cut(s) 151, 500, 1568
Mox20I TGGCCA 1 cut(s) 879
Mph1103I ATGCAT 1 cut(s) 1351
MroXI GAANNNNTTC 1 cut(s) 1053
MscI TGGCCA 1 cut(s) 879
MseI TTAA 2 cut(s) 366, 675
MslI CAYNNNNRTG 1 cut(s) 1412
Msp20I TGGCCA 1 cut(s) 879
MspA1I CMGCKG 1 cut(s) 1241
MspI CCGG 1 cut(s) 1403
MspR9I CCNGG 4 cut(s) 182, 1127, 1403, 1404
MunI CAATTG 2 cut(s) 756, 1389
Mva1269I GAATGC 2 cut(s) 1123, 1175
MvaI CCWGG 2 cut(s) 182, 1127
MvnI CGCG 2 cut(s) 629, 1059
MwoI GCNNNNNNNGC 7 cut(s) 169, 260, 822, 876, 1094, 1118, 1370
NciI CCSGG 2 cut(s) 1403, 1404
NdeII GATC 6 cut(s) 121, 441, 497, 578, 590, 1530
NlaIII CATG 5 cut(s) 18, 320, 797, 937, 1433
NlaIV GGNNCC 2 cut(s) 592, 1485
NmuCI GTSAC 2 cut(s) 1130, 1354
NruI TCGCGA 1 cut(s) 1059
NsiI ATGCAT 1 cut(s) 1351
NspI RCATGY 1 cut(s) 937
PagI TCATGA 1 cut(s) 1429
PctI GAATGC 2 cut(s) 1123, 1175
PdmI GAANNNNTTC 1 cut(s) 1053
PfeI GAWTC 8 cut(s) 77, 287, 322, 547, 690, 967, 1027, 1553
PflMI CCANNNNNTGG 1 cut(s) 704
PkrI GCNGC 3 cut(s) 872, 984, 1240
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PsiI TTATAA 1 cut(s) 555
Psp6I CCWGG 2 cut(s) 180, 1125
PspFI CCCAGC 2 cut(s) 377, 419
PspGI CCWGG 2 cut(s) 180, 1125
PspN4I GGNNCC 2 cut(s) 592, 1485
PspPI GGNCC 2 cut(s) 184, 761
PstI CTGCAG 1 cut(s) 1423
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 2 cut(s) 441, 590
PvuII CAGCTG 1 cut(s) 1241
RruI TCGCGA 1 cut(s) 1059
RsaI GTAC 2 cut(s) 301, 1445
RsaNI GTAC 2 cut(s) 300, 1444
RseI CAYNNNNRTG 1 cut(s) 1412
SaqAI TTAA 2 cut(s) 366, 675
SatI GCNGC 3 cut(s) 871, 983, 1239
Sau3AI GATC 6 cut(s) 121, 441, 497, 578, 590, 1530
Sau96I GGNCC 2 cut(s) 184, 761
SbfI CCTGCAGG 1 cut(s) 1423
SchI GAGTC 1 cut(s) 228
ScrFI CCNGG 4 cut(s) 182, 1127, 1403, 1404
SdaI CCTGCAGG 1 cut(s) 1423
SduI GDGCHC 1 cut(s) 529
SfaNI GCATC 7 cut(s) 229, 406, 775, 946, 1336, 1423, 1578
SfcI CTRYAG 4 cut(s) 402, 504, 777, 1419
SinI GGWCC 1 cut(s) 184
SmaI CCCGGG 1 cut(s) 1404
SmiMI CAYNNNNRTG 1 cut(s) 1412
SmlI CTYRAG 2 cut(s) 249, 947
SmoI CTYRAG 2 cut(s) 249, 947
SpeI ACTAGT 1 cut(s) 713
Sse8387I CCTGCAGG 1 cut(s) 1423
SsiI CCGC 4 cut(s) 594, 870, 944, 1371
SspMI CTAG 7 cut(s) 411, 566, 632, 714, 894, 1305, 1598
StyD4I CCNGG 4 cut(s) 180, 1125, 1401, 1402
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 4 cut(s) 115, 703, 778, 1549
TaqI TCGA 3 cut(s) 117, 718, 1515
TaqII GACCGA 1 cut(s) 659
TauI GCSGC 1 cut(s) 873
TfiI GAWTC 8 cut(s) 77, 287, 322, 547, 690, 967, 1027, 1553
Tru1I TTAA 2 cut(s) 366, 675
Tru9I TTAA 2 cut(s) 366, 675
TscAI CASTG 4 cut(s) 283, 309, 870, 1002
TseFI GTSAC 2 cut(s) 1130, 1354
TseI GCWGC 2 cut(s) 982, 1238
Tsp45I GTSAC 2 cut(s) 1130, 1354
TspDTI ATGAA 3 cut(s) 305, 549, 782
TspGWI ACGGA 1 cut(s) 653
TspMI CCCGGG 1 cut(s) 1402
TspRI CASTG 4 cut(s) 283, 309, 870, 1002
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 3 cut(s) 281, 1100, 1455
XbaI TCTAGA 1 cut(s) 1304
XceI RCATGY 1 cut(s) 937
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmaI CCCGGG 1 cut(s) 1402
XmnI GAANNNNTTC 1 cut(s) 1053
XspI CTAG 7 cut(s) 411, 566, 632, 714, 894, 1305, 1598
Zsp2I ATGCAT 1 cut(s) 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.