Rmu_co8319583.1_g000001

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8319583.1
OG0000427
Physical Location & Seq
Reverse (-)
2 .. 873
872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8319583.1_g000001.1.cds

Sequence Viewer

Length: 449 bp
atgaacaccacagccggctcttttgcgctgtttggatctgtcctgcctcgtgatgccggcgtggtggagaagttacggaaagcaggggctatcattttgggaaaggctagcttgagcgagtggtcacattttaggtctactaaaaccccctctggttggagcgctagaggcggttatggcttgaacccttacacattctcagacccttgcggctcaagcagcggttcagcaatatcagtggcagcaaatctggcagcggtgtcattaggtactgagacaagtggttcaatcttatgtccatcaagtttgaactcagttgtgggcctcaaaccaacagtcggtcttactagtcgagcaggggttgtcccaatttctctaagacaggatactgttgggacagtagcagatgctgcttatgttcttgatgccatagcagggatcgaccacaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

149

Amino Acids

15.0

Weight (kDa)

8.68

Isoelectric Point (pI)

23.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 137
AciI CCGC 4 cut(s) 171, 210, 222, 257
AclWI GGATC 2 cut(s) 43, 446
AfaI GTAC 1 cut(s) 271
AfeI AGCGCT 1 cut(s) 163
AfiI CCNNNNNNNGG 3 cut(s) 156, 338, 435
AgsI TTSAA 3 cut(s) 184, 288, 310
AhlI ACTAGT 1 cut(s) 347
AluBI AGCT 1 cut(s) 111
AluI AGCT 1 cut(s) 111
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 2 cut(s) 43, 446
AlwNI CAGNNNCTG 1 cut(s) 410
Aor51HI AGCGCT 1 cut(s) 163
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 4 cut(s) 219, 242, 254, 410
ArsI GACNNNNNNTTYG 2 cut(s) 321, 353
AspLEI GCGC 2 cut(s) 28, 164
AspS9I GGNCC 1 cut(s) 322
AsuNHI GCTAGC 1 cut(s) 107
BauI CACGAG 1 cut(s) 48
BbvI GCAGC 4 cut(s) 231, 254, 266, 397
BccI CCATC 1 cut(s) 307
BciVI GTATCC 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 269
BcuI ACTAGT 1 cut(s) 347
BfaI CTAG 3 cut(s) 108, 165, 348
BfoI RGCGCY 1 cut(s) 165
BfuI GTATCC 1 cut(s) 379
BisI GCNGC 5 cut(s) 211, 220, 243, 255, 411
BlsI GCNGC 5 cut(s) 212, 221, 244, 256, 412
BmgT120I GGNCC 1 cut(s) 322
BmsI GCATC 3 cut(s) 43, 397, 415
BmtI GCTAGC 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 133, 199
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 338, 435
Bse118I RCCGGY 2 cut(s) 14, 56
BseLI CCNNNNNNNGG 3 cut(s) 156, 338, 435
BseMII CTCAG 3 cut(s) 213, 264, 327
BseXI GCAGC 4 cut(s) 231, 254, 266, 397
BshFI GGCC 1 cut(s) 324
BsiSI CCGG 2 cut(s) 15, 57
BslFI GGGAC 2 cut(s) 350, 409
BslI CCNNNNNNNGG 3 cut(s) 156, 338, 435
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 350, 409
BsnI GGCC 1 cut(s) 324
Bsp143I GATC 2 cut(s) 35, 438
BspACI CCGC 4 cut(s) 171, 210, 222, 257
BspANI GGCC 1 cut(s) 324
BspCNI CTCAG 3 cut(s) 212, 265, 326
BspOI GCTAGC 1 cut(s) 111
BspPI GGATC 2 cut(s) 43, 446
BsrFI RCCGGY 2 cut(s) 14, 56
BssAI RCCGGY 2 cut(s) 14, 56
BssMI GATC 2 cut(s) 35, 438
BssSI CACGAG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 337, 391, 400
BstAPI GCANNNNNTGC 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 16, 58, 109
BstDEI CTNAG 4 cut(s) 199, 273, 313, 377
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 2 cut(s) 28, 164
BstKTI GATC 2 cut(s) 38, 441
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 2 cut(s) 35, 438
BstMWI GCNNNNNNNGC 6 cut(s) 168, 177, 216, 219, 251, 410
BstV1I GCAGC 4 cut(s) 231, 254, 266, 397
BstX2I RGATCY 1 cut(s) 35
BstYI RGATCY 1 cut(s) 35
BsuI GTATCC 1 cut(s) 379
BsuRI GGCC 1 cut(s) 324
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 3 cut(s) 16, 58, 109
CaiI CAGNNNCTG 1 cut(s) 410
CfoI GCGC 2 cut(s) 28, 164
Cfr10I RCCGGY 2 cut(s) 14, 56
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 1 cut(s) 270
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviJI RGCY 8 cut(s) 14, 18, 89, 107, 111, 180, 213, 324
CviKI_1 RGCY 8 cut(s) 14, 18, 89, 107, 111, 180, 213, 324
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 4 cut(s) 199, 273, 313, 377
DpnI GATC 2 cut(s) 37, 440
DpnII GATC 2 cut(s) 35, 438
Eco47III AGCGCT 1 cut(s) 163
FaiI YATR 4 cut(s) 177, 295, 417, 431
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 2 cut(s) 350, 409
FblI GTMKAC 1 cut(s) 137
Fnu4HI GCNGC 5 cut(s) 211, 220, 243, 255, 411
Fsp4HI GCNGC 5 cut(s) 211, 220, 243, 255, 411
FspBI CTAG 3 cut(s) 108, 165, 348
GlaI GCGC 2 cut(s) 27, 163
GluI GCNGC 5 cut(s) 211, 220, 243, 255, 411
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 1 cut(s) 324
HapII CCGG 2 cut(s) 15, 57
HhaI GCGC 2 cut(s) 28, 164
Hin6I GCGC 2 cut(s) 26, 162
HinP1I GCGC 2 cut(s) 26, 162
HpaII CCGG 2 cut(s) 15, 57
Hpy166II GTNNAC 1 cut(s) 138
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 2 cut(s) 50, 422
Hpy8I GTNNAC 1 cut(s) 138
HpyCH4III ACNGT 3 cut(s) 337, 391, 400
HpyF10VI GCNNNNNNNGC 6 cut(s) 168, 177, 216, 219, 251, 410
HpyF3I CTNAG 4 cut(s) 199, 273, 313, 377
HspAI GCGC 2 cut(s) 26, 162
KroI GCCGGC 2 cut(s) 14, 56
KroNI GCCGGC 2 cut(s) 16, 58
Kzo9I GATC 2 cut(s) 35, 438
LmnI GCTCC 1 cut(s) 159
LpnPI CCDG 9 cut(s) 28, 56, 69, 70, 138, 236, 342, 368, 420
Lsp1109I GCAGC 4 cut(s) 231, 254, 266, 397
LweI GCATC 3 cut(s) 43, 397, 415
MaeI CTAG 3 cut(s) 108, 165, 348
MaeIII GTNAC 2 cut(s) 72, 123
MalI GATC 2 cut(s) 37, 440
MboI GATC 2 cut(s) 35, 438
MflI RGATCY 1 cut(s) 35
MluCI AATT 1 cut(s) 369
MmeI TCCRAC 1 cut(s) 137
MnlI CCTC 4 cut(s) 57, 160, 161, 335
MroNI GCCGGC 2 cut(s) 14, 56
MspA1I CMGCKG 2 cut(s) 222, 257
MspI CCGG 2 cut(s) 15, 57
MwoI GCNNNNNNNGC 6 cut(s) 168, 177, 216, 219, 251, 410
NaeI GCCGGC 2 cut(s) 16, 58
NdeII GATC 2 cut(s) 35, 438
NgoMIV GCCGGC 2 cut(s) 14, 56
NheI GCTAGC 1 cut(s) 107
NmuCI GTSAC 1 cut(s) 123
PdiI GCCGGC 2 cut(s) 16, 58
PkrI GCNGC 5 cut(s) 212, 221, 244, 256, 412
PspPI GGNCC 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 1 cut(s) 35
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
SatI GCNGC 5 cut(s) 211, 220, 243, 255, 411
Sau3AI GATC 2 cut(s) 35, 438
Sau96I GGNCC 1 cut(s) 322
SetI ASST 3 cut(s) 113, 137, 271
SfaNI GCATC 3 cut(s) 43, 397, 415
SmlI CTYRAG 2 cut(s) 112, 214
SmoI CTYRAG 2 cut(s) 112, 214
SpeI ACTAGT 1 cut(s) 347
Sse9I AATT 1 cut(s) 369
SsiI CCGC 4 cut(s) 171, 210, 222, 257
SspMI CTAG 3 cut(s) 108, 165, 348
TaaI ACNGT 3 cut(s) 337, 391, 400
TaqI TCGA 2 cut(s) 352, 441
TaqII GACCGA 1 cut(s) 329
TasI AATT 1 cut(s) 369
TauI GCSGC 1 cut(s) 213
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 4 cut(s) 219, 242, 254, 410
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 1 cut(s) 243
XmiI GTMKAC 1 cut(s) 137
XspI CTAG 3 cut(s) 108, 165, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.