Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000427

Physical Location & Seq

Genomic Coordinates Forward (+)

19696008 .. 19699349

Gene Structure 3342 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1120300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1662 bp

ATGGCAGCTGGGGATGACTTTCCCTTGACGGGTGGAGTGTCGCTCGGGACACAAGCAACTACAGTCCATGGATTCTCAATCAAGGAAGCCACCATAGACGATCTCCAACTCGCTTTTGAGCACAACCAACTCACCTCAAGGCAACTTGTCCAATTTTACCTTTCTGAAATCAGTAGCCTCAACCCTTTCCTTAAGGCGGTCATAGAAGTGAACCCGGATGCGCTTGACCTTGCTGACAAGGCTGACTATGAGCGCAAGACTAAGGCGCCGTCCGCAACACTCTCCAAGTTACATGGCATTCCCATTCTTGTCAAGGACAACATTGCAACCAAGGACAAGTTGAACACCACAGCTGGCTCTTATGCTTTGGTTGGCTCAGTTGTGCCGCGTGATGCGGGTGTTGTCACCAAGCTGAGGAATGCCGGAGCTATCATACTGGGAAAGGCCAGCTTGAGCGAGTGGTCGAATTGGAGGTCCAATTGTGCTCCTAACGGTTGGAGTGCCAGAGGCGGCCTAGGGCGGAATCCTTACAATATTGAGGTGACGCCTAGTGGCTCAAGCAGCGGGTCAGCAATATCGGTGGCAGCAAATCTGGCAGCAGTGGCACTGGGGACTGAGACAGATGGGTCAATTTTAAGTCCGTCCAGTTTTAACTCAGTAGTTGGCATGAAACCAACAGTCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATCTCCTCAAGACAGGACTCAGTTGGGCCCATTTGCAGGACAGTATCAGATGCTGCTTATGTTCTTGATGTAATCGCAGGCATTGACATCAACGATATTGCAACAACTGAGGCATTGCAGTACATCCCTGATGGTGGCTACGCACAGTTTCTCAAACTTGATGGACTTAAAGGAAAGAGATTGGGGATATTGAGAAAATTCTATGACTTAAATGATACTTTCTTGACCCAAACTTTTGAACAACATGTCAACACATTGAGGAAAGGAGGTGCTGCTGTGGTAGACAATTTGAAAATGGCTCATGAGGTCAATTTCCGTGCAAGTGGCGAACACGCTGCGTTGTCATTTGAGTTCAAAGTATCCTTAAACAGATACCTTAATGACTTGGTGGATTCCCCGGTGCGATCCTTGGCAGACGTGATTGCCTTCAACAGGAAGAACTTGAAACTGGAAAAAATCGATGAATACGGACAAGAGCTCTTCGAAGCATCCGAGAAGACTAATGGAATAGGGGAAAAAGAGCAGAAACTGTTGTCAAAGTTAGCGACACTGTCGAGAGATGGATTTGAGAAGTTCATGACAGATAACAGACTAGATGCCTTGGTGACCTATTCTAGCAGTGCGTCTTCTATCCTTGCAATTGGTGGTTTCCCGGGCATTGTTGTGCCAGCGGGATATCATGACACTGAAAACTACCCTTTTGGCATTTGCTTTGGGGGACTAAAGGGCTCGGAGCCAACGTTGATTGAGGTCGCGTATGCATTTGAGAAAGCAACTAAGATCAGGAAGCCTCCGCCTCTAGACCAACTTATCTCAAGTACCGCCACTCCTACTGCTCCCGATCAAGTTGAGTCCGCGACATCCACCATCGCTGCAGCCACAGAAAGACTCGGCCTAGCCGCTCAAGAGCACCCCCAAGGAAGTCTCTTGCAAGAAACTGGTCAAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase

Protein Analysis

554

Amino Acids

58.9

Weight (kDa)

5.54

Isoelectric Point (pI)

32.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	49 - 440	2.8e-71	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34880 AT4G34880
fragaria_vesca	FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica	MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica	Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis	pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis	RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata	RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora	Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii	Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa	Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha	Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana	Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	625
AccB1I	GGYRCC	1 cut(s)	265
AccBSI	CCGCTC	1 cut(s)	1613
AccI	GTMKAC	1 cut(s)	993
AccII	CGCG	3 cut(s)	388, 1466, 1568
AclI	AACGTT	1 cut(s)	1451
AclWI	GGATC	1 cut(s)	1110
AcsI	RAATTY	1 cut(s)	908
AcyI	GRCGYC	2 cut(s)	266, 545
AfaI	GTAC	2 cut(s)	833, 1531
AfiI	CCNNNNNNNGG	7 cut(s)	29, 196, 414, 680, 747, 845, 1143
AflII	CTTAAG	1 cut(s)	191
AflIII	ACRYGT	1 cut(s)	955
AgsI	TTSAA	6 cut(s)	343, 950, 1003, 1066, 1141, 1156
AhlI	ACTAGT	1 cut(s)	689
AjiI	CACGTC	1 cut(s)	1129
AluBI	AGCT	6 cut(s)	8, 353, 412, 428, 450, 1189
AluI	AGCT	6 cut(s)	8, 353, 412, 428, 450, 1189
Alw21I	GWGCWC	4 cut(s)	123, 487, 1191, 1623
Alw26I	GTCTC	2 cut(s)	611, 1640
AlwI	GGATC	1 cut(s)	1110
AlwNI	CAGNNNCTG	2 cut(s)	764, 1240
Ama87I	CYCGRG	2 cut(s)	44, 1363
AoxI	GGCC	4 cut(s)	444, 511, 737, 1603
ApaI	GGGCCC	1 cut(s)	741
ApeKI	GCWGC	9 cut(s)	5, 561, 584, 596, 764, 983, 1046, 1583, 1586
ApoI	RAATTY	1 cut(s)	908
ArsI	GACNNNNNNTTYG	2 cut(s)	458, 490
AspA2I	CCTAGG	1 cut(s)	514
AspLEI	GCGC	3 cut(s)	223, 255, 268
AspS9I	GGNCC	3 cut(s)	474, 737, 738
AsuC2I	CCSGG	4 cut(s)	215, 1109, 1364, 1365
AsuHPI	GGTGA	4 cut(s)	124, 397, 553, 1327
AsuII	TTCGAA	1 cut(s)	1194
AvaI	CYCGRG	2 cut(s)	44, 1363
AvaII	GGWCC	1 cut(s)	474
AvrII	CCTAGG	1 cut(s)	514
BaeGI	GKGCMC	1 cut(s)	741
BanI	GGYRCC	1 cut(s)	265
BanII	GRGCYC	3 cut(s)	741, 1191, 1442
BbsI	GAAGAC	2 cut(s)	1214, 1329
Bbv12I	GWGCWC	4 cut(s)	123, 487, 1191, 1623
BbvCI	CCTCAGC	1 cut(s)	413
BbvI	GCAGC	9 cut(s)	17, 573, 596, 608, 751, 970, 1033, 1570, 1598
BccI	CCATC	5 cut(s)	617, 836, 866, 1265, 1586
BceAI	ACGGC	1 cut(s)	253
BcgI	CGANNNNNNTGC	2 cut(s)	1028, 1062
BciVI	GTATCC	1 cut(s)	1081
BcnI	CCSGG	4 cut(s)	215, 1109, 1364, 1365
BcoDI	GTCTC	2 cut(s)	611, 1640
BcuI	ACTAGT	1 cut(s)	689
BfaI	CTAG	7 cut(s)	515, 549, 690, 1304, 1326, 1511, 1607
BfmI	CTRYAG	2 cut(s)	60, 1584
BfoI	RGCGCY	1 cut(s)	269
BfrI	CTTAAG	1 cut(s)	191
BfuI	GTATCC	1 cut(s)	1081
BlnI	CCTAGG	1 cut(s)	514
Bme1390I	CCNGG	4 cut(s)	215, 1109, 1364, 1365
Bme18I	GGWCC	1 cut(s)	474
BmeT110I	CYCGRG	2 cut(s)	44, 1363
BmgBI	CACGTC	1 cut(s)	1129
BmgT120I	GGNCC	3 cut(s)	474, 737, 738
BmiI	GGNNCC	3 cut(s)	267, 739, 1446
BmrFI	CCNGG	4 cut(s)	215, 1109, 1364, 1365
BmrI	ACTGGG	2 cut(s)	446, 617
BmsI	GCATC	5 cut(s)	208, 382, 751, 1208, 1297
BmuI	ACTGGG	2 cut(s)	446, 617
BpiI	GAAGAC	2 cut(s)	1214, 1329
BplI	GAGNNNNNCTC	2 cut(s)	27, 59
Bpu10I	CCTNAGC	1 cut(s)	413
Bpu14I	TTCGAA	1 cut(s)	1194
BpuEI	CTTGAG	6 cut(s)	121, 472, 541, 703, 1510, 1599
BpuMI	CCSGG	4 cut(s)	215, 1109, 1364, 1365
Bsa29I	ATCGAT	1 cut(s)	1170
BsaHI	GRCGYC	2 cut(s)	266, 545
BsaJI	CCNNGG	8 cut(s)	67, 330, 514, 1107, 1119, 1311, 1363, 1627
BsaXI	ACNNNNNCTCC	4 cut(s)	1436, 1466, 1522, 1552
Bsc4I	CCNNNNNNNGG	7 cut(s)	29, 196, 414, 680, 747, 845, 1143
Bse1I	ACTGG	5 cut(s)	441, 612, 645, 1164, 1654
Bse3DI	GCAATG	2 cut(s)	321, 824
BseCI	ATCGAT	1 cut(s)	1170
BseDI	CCNNGG	8 cut(s)	67, 330, 514, 1107, 1119, 1311, 1363, 1627
BseGI	GGATG	5 cut(s)	19, 223, 834, 1199, 1571
BseLI	CCNNNNNNNGG	7 cut(s)	29, 196, 414, 680, 747, 845, 1143
BseMI	GCAATG	2 cut(s)	321, 824
BseMII	CTCAG	6 cut(s)	390, 404, 606, 669, 744, 810
BseNI	ACTGG	5 cut(s)	441, 612, 645, 1164, 1654
BseRI	GAGGAG	1 cut(s)	706
BseSI	GKGCMC	1 cut(s)	741
BseXI	GCAGC	9 cut(s)	17, 573, 596, 608, 751, 970, 1033, 1570, 1598
BseYI	CCCAGC	1 cut(s)	8
Bsh1236I	CGCG	3 cut(s)	388, 1466, 1568
BshFI	GGCC	4 cut(s)	446, 513, 739, 1605
BshNI	GGYRCC	1 cut(s)	265
BshVI	ATCGAT	1 cut(s)	1170
BsiHKAI	GWGCWC	4 cut(s)	123, 487, 1191, 1623
BsiHKCI	CYCGRG	2 cut(s)	44, 1363
BsiSI	CCGG	4 cut(s)	215, 423, 1109, 1364
BslFI	GGGAC	4 cut(s)	61, 625, 692, 1443
BslI	CCNNNNNNNGG	7 cut(s)	29, 196, 414, 680, 747, 845, 1143
BsmAI	GTCTC	2 cut(s)	611, 1640
BsmFI	GGGAC	4 cut(s)	61, 625, 692, 1443
BsmI	GAATGC	2 cut(s)	297, 424
BsnI	GGCC	4 cut(s)	446, 513, 739, 1605
BsoBI	CYCGRG	2 cut(s)	44, 1363
Bsp119I	TTCGAA	1 cut(s)	1194
Bsp120I	GGGCCC	1 cut(s)	737
Bsp1286I	GDGCHC	6 cut(s)	123, 487, 741, 1191, 1442, 1623
Bsp143I	GATC	4 cut(s)	100, 1115, 1491, 1552
Bsp19I	CCATGG	1 cut(s)	67
BspANI	GGCC	4 cut(s)	446, 513, 739, 1605
BspCNI	CTCAG	6 cut(s)	389, 405, 607, 668, 743, 811
BspDI	ATCGAT	1 cut(s)	1170
BspFNI	CGCG	3 cut(s)	388, 1466, 1568
BspHI	TCATGA	3 cut(s)	1012, 1287, 1390
BspLI	GGNNCC	3 cut(s)	267, 739, 1446
BspMAI	CTGCAG	1 cut(s)	1588
BspPI	GGATC	1 cut(s)	1110
BspQI	GCTCTTC	1 cut(s)	1196
BspT104I	TTCGAA	1 cut(s)	1194
BspT107I	GGYRCC	1 cut(s)	265
BspTI	CTTAAG	1 cut(s)	191
BsrBI	CCGCTC	1 cut(s)	1613
BsrDI	GCAATG	2 cut(s)	321, 824
BsrI	ACTGG	5 cut(s)	441, 612, 645, 1164, 1654
BssECI	CCNNGG	8 cut(s)	67, 330, 514, 1107, 1119, 1311, 1363, 1627
BssMI	GATC	4 cut(s)	100, 1115, 1491, 1552
BssNI	GRCGYC	2 cut(s)	266, 545
BssT1I	CCWWGG	6 cut(s)	67, 330, 514, 1119, 1311, 1627
Bst4CI	ACNGT	7 cut(s)	64, 494, 679, 754, 858, 1242, 1263
Bst6I	CTCTTC	1 cut(s)	1196
BstACI	GRCGYC	2 cut(s)	266, 545
BstAFI	CTTAAG	1 cut(s)	191
BstBI	TTCGAA	1 cut(s)	1194
BstC8I	GCNNGC	4 cut(s)	355, 448, 790, 1380
BstDEI	CTNAG	8 cut(s)	261, 376, 413, 615, 655, 730, 819, 1488
BstDSI	CCRYGG	1 cut(s)	67
BstEII	GGTNACC	1 cut(s)	1315
BstENI	CCTNNNNNAGG	1 cut(s)	1141
BstF5I	GGATG	5 cut(s)	19, 223, 834, 1199, 1571
BstFNI	CGCG	3 cut(s)	388, 1466, 1568
BstH2I	RGCGCY	1 cut(s)	269
BstHHI	GCGC	3 cut(s)	223, 255, 268
BstKTI	GATC	4 cut(s)	103, 1118, 1494, 1555
BstMAI	GTCTC	2 cut(s)	611, 1640
BstMBI	GATC	4 cut(s)	100, 1115, 1491, 1552
BstMWI	GCNNNNNNNGC	5 cut(s)	239, 272, 561, 593, 602
BstNSI	RCATGY	1 cut(s)	959
BstPI	GGTNACC	1 cut(s)	1315
BstSCI	CCNGG	4 cut(s)	213, 1107, 1362, 1363
BstSFI	CTRYAG	2 cut(s)	60, 1584
BstSLI	GKGCMC	1 cut(s)	741
BstUI	CGCG	3 cut(s)	388, 1466, 1568
BstV1I	GCAGC	9 cut(s)	17, 573, 596, 608, 751, 970, 1033, 1570, 1598
BstV2I	GAAGAC	2 cut(s)	1214, 1329
Bsu15I	ATCGAT	1 cut(s)	1170
BsuI	GTATCC	1 cut(s)	1081
BsuRI	GGCC	4 cut(s)	446, 513, 739, 1605
BsuTUI	ATCGAT	1 cut(s)	1170
BtgI	CCRYGG	1 cut(s)	67
BtgZI	GCGATG	1 cut(s)	1564
BtrI	CACGTC	1 cut(s)	1129
BtsCI	GGATG	5 cut(s)	19, 223, 834, 1199, 1571
BtsI	GCAGTG	2 cut(s)	606, 1336
BtsIMutI	CAGTG	5 cut(s)	605, 606, 1259, 1336, 1395
Cac8I	GCNNGC	4 cut(s)	355, 448, 790, 1380
CaiI	CAGNNNCTG	2 cut(s)	764, 1240
CciI	TCATGA	3 cut(s)	1012, 1287, 1390
CfoI	GCGC	3 cut(s)	223, 255, 268
Cfr13I	GGNCC	3 cut(s)	474, 737, 738
Cfr9I	CCCGGG	1 cut(s)	1363
ClaI	ATCGAT	1 cut(s)	1170
CseI	GACGC	2 cut(s)	553, 1323
Csp6I	GTAC	2 cut(s)	832, 1530
CspCI	CAANNNNNGTGG	2 cut(s)	561, 596
CviAII	CATG	7 cut(s)	68, 293, 667, 956, 1013, 1288, 1391
CviQI	GTAC	2 cut(s)	832, 1530
DdeI	CTNAG	8 cut(s)	261, 376, 413, 615, 655, 730, 819, 1488
DinI	GGCGCC	1 cut(s)	267
DpnI	GATC	4 cut(s)	102, 1117, 1493, 1554
DpnII	GATC	4 cut(s)	100, 1115, 1491, 1552
DrdI	GACNNNNNNGTC	1 cut(s)	625
DseDI	GACNNNNNNGTC	1 cut(s)	625
Eam1104I	CTCTTC	1 cut(s)	1196
EarI	CTCTTC	1 cut(s)	1196
EciI	GGCGGA	2 cut(s)	535, 1494
Ecl136II	GAGCTC	1 cut(s)	1189
Eco130I	CCWWGG	6 cut(s)	67, 330, 514, 1119, 1311, 1627
Eco24I	GRGCYC	3 cut(s)	741, 1191, 1442
Eco32I	GATATC	1 cut(s)	1388
Eco47I	GGWCC	1 cut(s)	474
Eco53kI	GAGCTC	1 cut(s)	1189
Eco88I	CYCGRG	2 cut(s)	44, 1363
Eco91I	GGTNACC	1 cut(s)	1315
EcoICRI	GAGCTC	1 cut(s)	1189
EcoNI	CCTNNNNNAGG	1 cut(s)	1141
EcoO65I	GGTNACC	1 cut(s)	1315
EcoRV	GATATC	1 cut(s)	1388
EcoT14I	CCWWGG	6 cut(s)	67, 330, 514, 1119, 1311, 1627
EcoT22I	ATGCAT	1 cut(s)	1474
EcoT38I	GRGCYC	3 cut(s)	741, 1191, 1442
EgeI	GGCGCC	1 cut(s)	267
EheI	GGCGCC	1 cut(s)	267
ErhI	CCWWGG	6 cut(s)	67, 330, 514, 1119, 1311, 1627
FaeI	CATG	7 cut(s)	71, 296, 670, 959, 1016, 1291, 1394
FalI	AAGNNNNNCTT	2 cut(s)	434, 466
FaqI	GGGAC	4 cut(s)	61, 625, 692, 1443
FatI	CATG	7 cut(s)	67, 292, 666, 955, 1012, 1287, 1390
FauI	CCCGC	3 cut(s)	388, 557, 1375
FblI	GTMKAC	1 cut(s)	993
FokI	GGATG	5 cut(s)	26, 230, 821, 1186, 1558
FriOI	GRGCYC	3 cut(s)	741, 1191, 1442
FspBI	CTAG	7 cut(s)	515, 549, 690, 1304, 1326, 1511, 1607
GlaI	GCGC	3 cut(s)	222, 254, 267
GsaI	CCCAGC	1 cut(s)	12
HaeII	RGCGCY	1 cut(s)	269
HaeIII	GGCC	4 cut(s)	446, 513, 739, 1605
HapII	CCGG	4 cut(s)	215, 423, 1109, 1364
HgaI	GACGC	2 cut(s)	553, 1323
HhaI	GCGC	3 cut(s)	223, 255, 268
Hin1I	GRCGYC	2 cut(s)	266, 545
Hin1II	CATG	7 cut(s)	71, 296, 670, 959, 1016, 1291, 1394
Hin6I	GCGC	3 cut(s)	221, 253, 266
HinP1I	GCGC	3 cut(s)	221, 253, 266
HincII	GTYRAC	1 cut(s)	961
HindII	GTYRAC	1 cut(s)	961
HinfI	GANTC	6 cut(s)	72, 523, 728, 1103, 1562, 1599
HpaII	CCGG	4 cut(s)	215, 423, 1109, 1364
HphI	GGTGA	4 cut(s)	124, 397, 553, 1327
Hpy166II	GTNNAC	3 cut(s)	211, 961, 994
Hpy188I	TCNGA	4 cut(s)	166, 760, 1204, 1444
Hpy8I	GTNNAC	3 cut(s)	211, 961, 994
HpyAV	CCTTC	1 cut(s)	1147
HpyCH4III	ACNGT	7 cut(s)	64, 494, 679, 754, 858, 1242, 1263
HpyCH4IV	ACGT	2 cut(s)	1128, 1451
HpyCH4V	TGCA	9 cut(s)	326, 747, 812, 829, 1031, 1349, 1472, 1586, 1642
HpyF10VI	GCNNNNNNNGC	5 cut(s)	239, 272, 561, 593, 602
HpyF3I	CTNAG	8 cut(s)	261, 376, 413, 615, 655, 730, 819, 1488
HpySE526I	ACGT	2 cut(s)	1128, 1451
Hsp92I	GRCGYC	2 cut(s)	266, 545
Hsp92II	CATG	7 cut(s)	71, 296, 670, 959, 1016, 1291, 1394
HspAI	GCGC	3 cut(s)	221, 253, 266
KasI	GGCGCC	1 cut(s)	265
Kzo9I	GATC	4 cut(s)	100, 1115, 1491, 1552
LguI	GCTCTTC	1 cut(s)	1196
LmnI	GCTCC	4 cut(s)	425, 490, 1444, 1552
Lsp1109I	GCAGC	9 cut(s)	17, 573, 596, 608, 751, 970, 1033, 1570, 1598
LweI	GCATC	5 cut(s)	208, 382, 751, 1208, 1297
MaeI	CTAG	7 cut(s)	515, 549, 690, 1304, 1326, 1511, 1607
MaeII	ACGT	2 cut(s)	1128, 1451
MaeIII	GTNAC	4 cut(s)	288, 403, 541, 1315
MalI	GATC	4 cut(s)	102, 1117, 1493, 1554
MbiI	CCGCTC	1 cut(s)	1613
MboI	GATC	4 cut(s)	100, 1115, 1491, 1552
MboII	GAAGA	4 cut(s)	1159, 1183, 1219, 1329
MfeI	CAATTG	2 cut(s)	478, 1350
MhlI	GDGCHC	6 cut(s)	123, 487, 741, 1191, 1442, 1623
MluCI	AATT	8 cut(s)	152, 466, 478, 630, 908, 997, 1021, 1350
Mly113I	GGCGCC	1 cut(s)	266
MlyI	GAGTC	3 cut(s)	722, 1571, 1593
MmeI	TCCRAC	2 cut(s)	130, 476
Mph1103I	ATGCAT	1 cut(s)	1474
MseI	TTAA	7 cut(s)	192, 635, 651, 879, 920, 1076, 1089
MslI	CAYNNNNRTG	2 cut(s)	206, 1373
MspA1I	CMGCKG	4 cut(s)	8, 353, 564, 1382
MspCI	CTTAAG	1 cut(s)	191
MspI	CCGG	4 cut(s)	215, 423, 1109, 1364
MspR9I	CCNGG	4 cut(s)	215, 1109, 1364, 1365
MunI	CAATTG	2 cut(s)	478, 1350
Mva1269I	GAATGC	2 cut(s)	297, 424
MvnI	CGCG	3 cut(s)	388, 1466, 1568
MwoI	GCNNNNNNNGC	5 cut(s)	239, 272, 561, 593, 602
NarI	GGCGCC	1 cut(s)	266
NciI	CCSGG	4 cut(s)	215, 1109, 1364, 1365
NcoI	CCATGG	1 cut(s)	67
NdeII	GATC	4 cut(s)	100, 1115, 1491, 1552
NlaIII	CATG	7 cut(s)	71, 296, 670, 959, 1016, 1291, 1394
NlaIV	GGNNCC	3 cut(s)	267, 739, 1446
NmeAIII	GCCGAG	1 cut(s)	1581
NmuCI	GTSAC	3 cut(s)	403, 541, 1315
NsiI	ATGCAT	1 cut(s)	1474
NspI	RCATGY	1 cut(s)	959
NspV	TTCGAA	1 cut(s)	1194
PagI	TCATGA	3 cut(s)	1012, 1287, 1390
PciI	ACATGT	1 cut(s)	955
PciSI	GCTCTTC	1 cut(s)	1196
PcsI	WCGNNNNNNNCGW	2 cut(s)	1200, 1448
PctI	GAATGC	2 cut(s)	297, 424
PfeI	GAWTC	3 cut(s)	72, 523, 1103
PflFI	GACNNNGTC	1 cut(s)	1261
PleI	GAGTC	3 cut(s)	722, 1570, 1593
PluTI	GGCGCC	1 cut(s)	269
PpsI	GAGTC	3 cut(s)	722, 1570, 1593
PscI	ACATGT	1 cut(s)	955
Psp124BI	GAGCTC	1 cut(s)	1191
Psp1406I	AACGTT	1 cut(s)	1451
PspEI	GGTNACC	1 cut(s)	1315
PspFI	CCCAGC	1 cut(s)	8
PspN4I	GGNNCC	3 cut(s)	267, 739, 1446
PspOMI	GGGCCC	1 cut(s)	737
PspPI	GGNCC	3 cut(s)	474, 737, 738
PstI	CTGCAG	1 cut(s)	1588
PstNI	CAGNNNCTG	2 cut(s)	764, 1240
PsyI	GACNNNGTC	1 cut(s)	1261
PvuII	CAGCTG	2 cut(s)	8, 353
RsaI	GTAC	2 cut(s)	833, 1531
RsaNI	GTAC	2 cut(s)	832, 1530
RseI	CAYNNNNRTG	2 cut(s)	206, 1373
SacI	GAGCTC	1 cut(s)	1191
SapI	GCTCTTC	1 cut(s)	1196
SaqAI	TTAA	7 cut(s)	192, 635, 651, 879, 920, 1076, 1089
Sau3AI	GATC	4 cut(s)	100, 1115, 1491, 1552
Sau96I	GGNCC	3 cut(s)	474, 737, 738
SchI	GAGTC	3 cut(s)	722, 1571, 1593
ScrFI	CCNGG	4 cut(s)	215, 1109, 1364, 1365
SduI	GDGCHC	6 cut(s)	123, 487, 741, 1191, 1442, 1623
SfaNI	GCATC	5 cut(s)	208, 382, 751, 1208, 1297
SfcI	CTRYAG	2 cut(s)	60, 1584
SfoI	GGCGCC	1 cut(s)	267
SfuI	TTCGAA	1 cut(s)	1194
SinI	GGWCC	1 cut(s)	474
SmaI	CCCGGG	1 cut(s)	1365
SmiMI	CAYNNNNRTG	2 cut(s)	206, 1373
SmlI	CTYRAG	7 cut(s)	136, 191, 451, 556, 718, 1525, 1614
SmoI	CTYRAG	7 cut(s)	136, 191, 451, 556, 718, 1525, 1614
SpeI	ACTAGT	1 cut(s)	689
Sse9I	AATT	8 cut(s)	152, 466, 478, 630, 908, 997, 1021, 1350
SspDI	GGCGCC	1 cut(s)	265
SspI	AATATT	1 cut(s)	535
SspMI	CTAG	7 cut(s)	515, 549, 690, 1304, 1326, 1511, 1607
SstI	GAGCTC	1 cut(s)	1191
StyD4I	CCNGG	4 cut(s)	213, 1107, 1362, 1363
StyI	CCWWGG	6 cut(s)	67, 330, 514, 1119, 1311, 1627
TaaI	ACNGT	7 cut(s)	64, 494, 679, 754, 858, 1242, 1263
TaiI	ACGT	2 cut(s)	1131, 1454
TaqI	TCGA	5 cut(s)	464, 694, 1170, 1194, 1265
TaqII	GACCGA	1 cut(s)	671
TasI	AATT	8 cut(s)	152, 466, 478, 630, 908, 997, 1021, 1350
TatI	WGTACW	1 cut(s)	831
TauI	GCSGC	3 cut(s)	388, 513, 1613
TfiI	GAWTC	3 cut(s)	72, 523, 1103
Tru1I	TTAA	7 cut(s)	192, 635, 651, 879, 920, 1076, 1089
Tru9I	TTAA	7 cut(s)	192, 635, 651, 879, 920, 1076, 1089
TscAI	CASTG	5 cut(s)	606, 612, 1266, 1336, 1402
TseFI	GTSAC	3 cut(s)	403, 541, 1315
TseI	GCWGC	9 cut(s)	5, 561, 584, 596, 764, 983, 1046, 1583, 1586
Tsp45I	GTSAC	3 cut(s)	403, 541, 1315
TspDTI	ATGAA	3 cut(s)	683, 1188, 1276
TspGWI	ACGGA	3 cut(s)	630, 1016, 1194
TspMI	CCCGGG	1 cut(s)	1363
TspRI	CASTG	5 cut(s)	606, 612, 1266, 1336, 1402
Tth111I	GACNNNGTC	1 cut(s)	1261
Vha464I	CTTAAG	1 cut(s)	191
VpaK11BI	GGWCC	1 cut(s)	474
XagI	CCTNNNNNAGG	1 cut(s)	1141
XapI	RAATTY	1 cut(s)	908
XbaI	TCTAGA	1 cut(s)	1510
XceI	RCATGY	1 cut(s)	959
XmaI	CCCGGG	1 cut(s)	1363
XmaJI	CCTAGG	1 cut(s)	514
XmiI	GTMKAC	1 cut(s)	993
XspI	CTAG	7 cut(s)	515, 549, 690, 1304, 1326, 1511, 1607
Zsp2I	ATGCAT	1 cut(s)	1474

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.