Prupe.8G161600_v2.0.a1

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000427
Physical Location & Seq
Forward (+)
16971667 .. 16974002
2336 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G161600.1

Sequence Viewer

Length: 1560 bp
ATGGCACAACCAAGCCTCTCTCACTTCTCATCCTTCCTTTTGATTCTTCTTCTCTTTGCATTGTCACTTGGGCAACAAACAGCTGCAGGACATGGACTCTCAATCAAGGAAGCCACCATAGACGATCTCCAACTAGCTTTCAAGCACAACAAGCTCACCTCAAGGCAACTTGTCCAGTTTTACCTTGGGGAAATCAGCAAGCTCAACCCTTTCCTCAAGGGGGTCATAGAAGTGAACCCGGATGCGCTTTACCTTGCCGAAAAGGCTGACTACAAGCGCAAGACTAAGGCACCATCTGTGTCACTCTCTAAGTTACATGGCATTCCCATTCTGGTCAAGGACAACATTGCAACCAAGGACAAGTTGAACACCACAGCTGGCTCTTATGCTCTGCTTGGCTCTGTTGTGCCCCGGGACGCCGGCGTGGTGACCAAGTTGAGGAAGGCTGGGGCTGTCATACTGGGAAAGGCCAGCTTGAGTGAGTGGTCCTATTGGAGGTCCAATGATGCACCTTCTGGTTGGAGTGGCAGAGGTGGCCAAGGGGTGAACCCTTACAATTTTAGCACGACGCCAAGCGGCTCAAGTAGCGGGCCGGCAATATCAGCGGCAGCAAATCTGGCAACAGTGACACTGGGGAGTGAAACAGACGGGTCGATTTTAAGTCCATCCAGTTGGAACTCAGTGGTGGGCATCAAACCAACAATTGGCCTTACAAGTCGAGCAGGGGTTGTTCCAATCTCTCCAAGACAGGACACAGTCGGGCCAATTACTAGGACAGTATCAGATGCCGCATATGTTCTTGATGTCATTGCAGGCATTGACAGCAATGACCTTGCAACAAGTGAGGCATCGAGGTACATTCCCAAGGGCGGCTATGCACAATTTCTCAGACCTGATGGACTTAGAGGTAAGAGAATAGGGATAGTGAGAGCCTTGTTCAACTTTGGCAGTGACGCTTCGTTGACTCAAACTTTCGAACAGCATTTCAACACTCTGAGGAAAAGAGGTGCTGTTTTGGTAGACAATTTGGAAATAGCACATTTTGATGAAATCTACTATAATTCAAGTAGTGAAGACATTGCATTGTCTGCTGAGTTCAAAATTTACTTGAACACATACCTCAAGAACTTGGTGGCTTCACCGGTTCGATCCTTGGCAGACGTGATAGTCTTCAACAATAAAAACTCAAAACTGGAGAAAGTGAAGGAGTATGGCCAAGGCCTACTCTTGAAATCTGAAGCAACAAATGGGATTGGCAATGCAGAGAAGGCAGCGCTGGTAAACTTGGCAAAGCTATCCAAAAATGGTTTCGAGAAGCTCGTGACGAAGAAGAGGCTAGACGCAGTGGTGGCTCCATCTGCAACCGTGTCGACGTTGCTCGCCATAGCAGGCTCCCCAGGAGTGGTTGTCCCAGCAGGATATACTAAAGATGGGGTACCGTTTGGCATCTCCTTTGGCGGGCTGAGGGGTTCGGAGCCAAAGCTAATTGAGATAGCATACGGCTTTGAGCAAGCCACTAAGATTAGGAAGCCTCCTTCACTTAAAAACTTCAAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

520

Amino Acids

55.3

Weight (kDa)

9.61

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1166
Acc65I GGTACC 1 cut(s) 1435
AccB1I GGYRCC 2 cut(s) 289, 1435
AccB7I CCANNNNNTGG 1 cut(s) 704
AccI GTMKAC 2 cut(s) 1020, 1370
AciI CCGC 6 cut(s) 576, 588, 605, 789, 870, 1458
AclWI GGATC 1 cut(s) 1143
AcoI YGGCCR 2 cut(s) 535, 1213
AcsI RAATTY 1 cut(s) 1101
AcuI CTGAAG 1 cut(s) 1257
AcyI GRCGYC 2 cut(s) 417, 569
AfaI GTAC 2 cut(s) 857, 1437
AfeI AGCGCT 1 cut(s) 1275
AfiI CCNNNNNNNGG 7 cut(s) 220, 438, 495, 704, 869, 1402, 1458
AgeI ACCGGT 1 cut(s) 1141
AjiI CACGTC 1 cut(s) 1162
AjnI CCWGG 1 cut(s) 1396
AjuI GAANNNNNNNTTGG 2 cut(s) 1292, 1324
AluBI AGCT 9 cut(s) 83, 137, 154, 202, 377, 474, 1294, 1318, 1483
AluI AGCT 9 cut(s) 83, 137, 154, 202, 377, 474, 1294, 1318, 1483
AlwI GGATC 1 cut(s) 1143
Ama87I CYCGRG 1 cut(s) 411
Aor51HI AGCGCT 1 cut(s) 1275
AoxI GGCC 7 cut(s) 468, 535, 590, 706, 761, 1213, 1219
ApeKI GCWGC 3 cut(s) 83, 608, 1271
ApoI RAATTY 1 cut(s) 1101
AsiGI ACCGGT 1 cut(s) 1141
Asp718I GGTACC 1 cut(s) 1435
AspLEI GCGC 3 cut(s) 247, 279, 1276
AspS9I GGNCC 4 cut(s) 486, 498, 590, 761
AsuC2I CCSGG 3 cut(s) 239, 412, 413
AsuHPI GGTGA 4 cut(s) 148, 439, 556, 1131
AsuII TTCGAA 1 cut(s) 975
AvaI CYCGRG 1 cut(s) 411
AvaII GGWCC 2 cut(s) 486, 498
BaeGI GKGCMC 1 cut(s) 411
BalI TGGCCA 2 cut(s) 537, 1215
BanI GGYRCC 2 cut(s) 289, 1435
BauI CACGAG 1 cut(s) 1319
BbsI GAAGAC 2 cut(s) 1080, 1162
BbvCI CCTCAGC 1 cut(s) 1463
BbvI GCAGC 3 cut(s) 70, 620, 1283
BccI CCATC 5 cut(s) 301, 673, 890, 1363, 1424
BceAI ACGGC 1 cut(s) 1516
BcgI CGANNNNNNTGC 2 cut(s) 1350, 1384
BciT130I CCWGG 1 cut(s) 1398
BcnI CCSGG 3 cut(s) 239, 412, 413
BfaI CTAG 3 cut(s) 134, 771, 1337
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 1277
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 7 cut(s) 84, 577, 606, 609, 789, 871, 1272
BlsI GCNGC 7 cut(s) 85, 578, 607, 610, 790, 872, 1273
Bme1390I CCNGG 4 cut(s) 239, 412, 413, 1398
Bme18I GGWCC 2 cut(s) 486, 498
BmeT110I CYCGRG 1 cut(s) 411
BmgBI CACGTC 1 cut(s) 1162
BmgT120I GGNCC 4 cut(s) 486, 498, 590, 761
BmiI GGNNCC 5 cut(s) 291, 1353, 1393, 1437, 1476
BmrFI CCNGG 4 cut(s) 239, 412, 413, 1398
BmrI ACTGGG 2 cut(s) 470, 641
BmsI GCATC 6 cut(s) 232, 496, 699, 775, 857, 1455
BmuI ACTGGG 2 cut(s) 470, 641
BpiI GAAGAC 2 cut(s) 1080, 1162
BpmI CTGGAG 1 cut(s) 1214
Bpu10I CCTNAGC 1 cut(s) 1463
Bpu14I TTCGAA 1 cut(s) 975
BpuEI CTTGAG 5 cut(s) 145, 200, 496, 565, 1106
BpuMI CCSGG 3 cut(s) 239, 412, 413
BsaHI GRCGYC 2 cut(s) 417, 569
BsaJI CCNNGG 9 cut(s) 184, 354, 410, 411, 538, 864, 1152, 1216, 1396
BsaWI WCCGGW 1 cut(s) 1141
BsaXI ACNNNNNCTCC 2 cut(s) 1392, 1422
Bsc4I CCNNNNNNNGG 7 cut(s) 220, 438, 495, 704, 869, 1402, 1458
Bse118I RCCGGY 3 cut(s) 419, 592, 1141
Bse1I ACTGG 5 cut(s) 175, 465, 636, 669, 1197
Bse3DI GCAATG 5 cut(s) 345, 807, 832, 1077, 1264
BseBI CCWGG 1 cut(s) 1398
BseDI CCNNGG 9 cut(s) 184, 354, 410, 411, 538, 864, 1152, 1216, 1396
BseGI GGATG 3 cut(s) 29, 247, 665
BseLI CCNNNNNNNGG 7 cut(s) 220, 438, 495, 704, 869, 1402, 1458
BseMI GCAATG 5 cut(s) 345, 807, 832, 1077, 1264
BseMII CTCAG 5 cut(s) 693, 901, 986, 1083, 1454
BseNI ACTGG 5 cut(s) 175, 465, 636, 669, 1197
BseSI GKGCMC 1 cut(s) 411
BseXI GCAGC 3 cut(s) 70, 620, 1283
BseYI CCCAGC 2 cut(s) 446, 1411
BshFI GGCC 7 cut(s) 470, 537, 592, 708, 763, 1215, 1221
BshNI GGYRCC 2 cut(s) 289, 1435
BshTI ACCGGT 1 cut(s) 1141
BsiHKCI CYCGRG 1 cut(s) 411
BsiSI CCGG 5 cut(s) 239, 412, 420, 593, 1142
BslFI GGGAC 2 cut(s) 428, 1394
BslI CCNNNNNNNGG 7 cut(s) 220, 438, 495, 704, 869, 1402, 1458
BsmFI GGGAC 2 cut(s) 428, 1394
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 7 cut(s) 470, 537, 592, 708, 763, 1215, 1221
BsoBI CYCGRG 1 cut(s) 411
Bsp119I TTCGAA 1 cut(s) 975
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 2 cut(s) 124, 1148
BspACI CCGC 6 cut(s) 576, 588, 605, 789, 870, 1458
BspANI GGCC 7 cut(s) 470, 537, 592, 708, 763, 1215, 1221
BspCNI CTCAG 5 cut(s) 692, 900, 987, 1084, 1455
BspLI GGNNCC 5 cut(s) 291, 1353, 1393, 1437, 1476
BspMAI CTGCAG 1 cut(s) 88
BspPI GGATC 1 cut(s) 1143
BspT104I TTCGAA 1 cut(s) 975
BspT107I GGYRCC 2 cut(s) 289, 1435
BsrDI GCAATG 5 cut(s) 345, 807, 832, 1077, 1264
BsrFI RCCGGY 3 cut(s) 419, 592, 1141
BsrI ACTGG 5 cut(s) 175, 465, 636, 669, 1197
BssAI RCCGGY 3 cut(s) 419, 592, 1141
BssECI CCNNGG 9 cut(s) 184, 354, 410, 411, 538, 864, 1152, 1216, 1396
BssMI GATC 2 cut(s) 124, 1148
BssNI GRCGYC 2 cut(s) 417, 569
BssSI CACGAG 1 cut(s) 1319
BssT1I CCWWGG 6 cut(s) 184, 354, 538, 864, 1152, 1216
Bst2BI CACGAG 1 cut(s) 1319
Bst2UI CCWGG 1 cut(s) 1398
Bst4CI ACNGT 5 cut(s) 625, 757, 778, 1366, 1440
Bst6I CTCTTC 1 cut(s) 1325
BstACI GRCGYC 2 cut(s) 417, 569
BstAPI GCANNNNNTGC 1 cut(s) 1088
BstBI TTCGAA 1 cut(s) 975
BstDEI CTNAG 9 cut(s) 285, 309, 679, 887, 902, 995, 1092, 1463, 1518
BstEII GGTNACC 1 cut(s) 427
BstENI CCTNNNNNAGG 1 cut(s) 493
BstF5I GGATG 3 cut(s) 29, 247, 665
BstH2I RGCGCY 1 cut(s) 1277
BstHHI GCGC 3 cut(s) 247, 279, 1276
BstKTI GATC 2 cut(s) 127, 1151
BstMBI GATC 2 cut(s) 124, 1148
BstNI CCWGG 1 cut(s) 1398
BstPI GGTNACC 1 cut(s) 427
BstSCI CCNGG 4 cut(s) 237, 410, 411, 1396
BstSFI CTRYAG 1 cut(s) 84
BstSLI GKGCMC 1 cut(s) 411
BstV1I GCAGC 3 cut(s) 70, 620, 1283
BstV2I GAAGAC 2 cut(s) 1080, 1162
BstXI CCANNNNNNTGG 1 cut(s) 672
BsuRI GGCC 7 cut(s) 470, 537, 592, 708, 763, 1215, 1221
BtrI CACGTC 1 cut(s) 1162
BtsCI GGATG 3 cut(s) 29, 247, 665
BtsI GCAGTG 2 cut(s) 955, 1350
BtsIMutI CAGTG 5 cut(s) 629, 630, 687, 955, 1350
CfoI GCGC 3 cut(s) 247, 279, 1276
Cfr10I RCCGGY 3 cut(s) 419, 592, 1141
Cfr13I GGNCC 4 cut(s) 486, 498, 590, 761
Cfr9I CCCGGG 1 cut(s) 411
CseI GACGC 4 cut(s) 425, 577, 962, 1349
Csp6I GTAC 2 cut(s) 856, 1436
CspAI ACCGGT 1 cut(s) 1141
CviAII CATG 2 cut(s) 92, 317
CviQI GTAC 2 cut(s) 856, 1436
DdeI CTNAG 9 cut(s) 285, 309, 679, 887, 902, 995, 1092, 1463, 1518
DpnI GATC 2 cut(s) 126, 1150
DpnII GATC 2 cut(s) 124, 1148
DrdI GACNNNNNNGTC 1 cut(s) 1166
DseDI GACNNNNNNGTC 1 cut(s) 1166
EaeI YGGCCR 2 cut(s) 535, 1213
Eam1104I CTCTTC 1 cut(s) 1325
EarI CTCTTC 1 cut(s) 1325
Eco130I CCWWGG 6 cut(s) 184, 354, 538, 864, 1152, 1216
Eco147I AGGCCT 1 cut(s) 1221
Eco47I GGWCC 2 cut(s) 486, 498
Eco47III AGCGCT 1 cut(s) 1275
Eco57I CTGAAG 1 cut(s) 1257
Eco88I CYCGRG 1 cut(s) 411
Eco91I GGTNACC 1 cut(s) 427
EcoNI CCTNNNNNAGG 1 cut(s) 493
EcoO65I GGTNACC 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 1396
EcoT14I CCWWGG 6 cut(s) 184, 354, 538, 864, 1152, 1216
ErhI CCWWGG 6 cut(s) 184, 354, 538, 864, 1152, 1216
FaeI CATG 2 cut(s) 95, 320
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 2 cut(s) 428, 1394
FatI CATG 2 cut(s) 91, 316
FauI CCCGC 2 cut(s) 581, 1451
FauNDI CATATG 1 cut(s) 793
FblI GTMKAC 2 cut(s) 1020, 1370
Fnu4HI GCNGC 7 cut(s) 84, 577, 606, 609, 789, 871, 1272
FokI GGATG 3 cut(s) 16, 254, 652
Fsp4HI GCNGC 7 cut(s) 84, 577, 606, 609, 789, 871, 1272
FspBI CTAG 3 cut(s) 134, 771, 1337
GlaI GCGC 3 cut(s) 246, 278, 1275
GluI GCNGC 7 cut(s) 84, 577, 606, 609, 789, 871, 1272
GsaI CCCAGC 2 cut(s) 450, 1415
GsuI CTGGAG 1 cut(s) 1214
HaeII RGCGCY 1 cut(s) 1277
HaeIII GGCC 7 cut(s) 470, 537, 592, 708, 763, 1215, 1221
HapII CCGG 5 cut(s) 239, 412, 420, 593, 1142
HgaI GACGC 4 cut(s) 425, 577, 962, 1349
HhaI GCGC 3 cut(s) 247, 279, 1276
Hin1I GRCGYC 2 cut(s) 417, 569
Hin1II CATG 2 cut(s) 95, 320
Hin6I GCGC 3 cut(s) 245, 277, 1274
HinP1I GCGC 3 cut(s) 245, 277, 1274
HincII GTYRAC 2 cut(s) 963, 1371
HindII GTYRAC 2 cut(s) 963, 1371
HinfI GANTC 3 cut(s) 43, 96, 964
HpaII CCGG 5 cut(s) 239, 412, 420, 593, 1142
HphI GGTGA 4 cut(s) 148, 439, 556, 1131
Hpy166II GTNNAC 6 cut(s) 235, 547, 963, 1021, 1282, 1371
Hpy188I TCNGA 5 cut(s) 784, 890, 996, 1237, 1474
Hpy188III TCNNGA 6 cut(s) 800, 1123, 1228, 1312, 1321, 1552
Hpy8I GTNNAC 6 cut(s) 235, 547, 963, 1021, 1282, 1371
Hpy99I CGWCG 2 cut(s) 571, 1375
HpyAV CCTTC 6 cut(s) 43, 436, 522, 1198, 1261, 1545
HpyCH4III ACNGT 5 cut(s) 625, 757, 778, 1366, 1440
HpyCH4IV ACGT 2 cut(s) 1161, 1373
HpyF3I CTNAG 9 cut(s) 285, 309, 679, 887, 902, 995, 1092, 1463, 1518
HpySE526I ACGT 2 cut(s) 1161, 1373
Hsp92I GRCGYC 2 cut(s) 417, 569
Hsp92II CATG 2 cut(s) 95, 320
HspAI GCGC 3 cut(s) 245, 277, 1274
KpnI GGTACC 1 cut(s) 1439
KroI GCCGGC 2 cut(s) 419, 592
KroNI GCCGGC 2 cut(s) 421, 594
Kzo9I GATC 2 cut(s) 124, 1148
LmnI GCTCC 3 cut(s) 1357, 1397, 1474
Lsp1109I GCAGC 3 cut(s) 70, 620, 1283
LweI GCATC 6 cut(s) 232, 496, 699, 775, 857, 1455
MaeI CTAG 3 cut(s) 134, 771, 1337
MaeII ACGT 2 cut(s) 1161, 1373
MaeIII GTNAC 7 cut(s) 63, 300, 312, 427, 625, 950, 1321
MalI GATC 2 cut(s) 126, 1150
MboI GATC 2 cut(s) 124, 1148
MboII GAAGA 6 cut(s) 38, 41, 1085, 1162, 1339, 1342
MfeI CAATTG 1 cut(s) 702
MhlI GDGCHC 1 cut(s) 411
MlsI TGGCCA 2 cut(s) 537, 1215
MluCI AATT 8 cut(s) 556, 702, 765, 881, 1024, 1060, 1101, 1485
MluNI TGGCCA 2 cut(s) 537, 1215
MlyI GAGTC 2 cut(s) 90, 958
MmeI TCCRAC 3 cut(s) 154, 500, 653
Mox20I TGGCCA 2 cut(s) 537, 1215
MreI CGCCGGCG 1 cut(s) 419
MroNI GCCGGC 2 cut(s) 419, 592
MscI TGGCCA 2 cut(s) 537, 1215
MseI TTAA 2 cut(s) 659, 1542
MslI CAYNNNNRTG 2 cut(s) 230, 1044
Msp20I TGGCCA 2 cut(s) 537, 1215
MspA1I CMGCKG 3 cut(s) 83, 377, 605
MspI CCGG 5 cut(s) 239, 412, 420, 593, 1142
MspR9I CCNGG 4 cut(s) 239, 412, 413, 1398
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 321
MvaI CCWGG 1 cut(s) 1398
NaeI GCCGGC 2 cut(s) 421, 594
NciI CCSGG 3 cut(s) 239, 412, 413
NdeI CATATG 1 cut(s) 793
NdeII GATC 2 cut(s) 124, 1148
NgoMIV GCCGGC 2 cut(s) 419, 592
NlaIII CATG 2 cut(s) 95, 320
NlaIV GGNNCC 5 cut(s) 291, 1353, 1393, 1437, 1476
NmuCI GTSAC 6 cut(s) 63, 300, 427, 625, 950, 1321
NspV TTCGAA 1 cut(s) 975
PceI AGGCCT 1 cut(s) 1221
PcsI WCGNNNNNNNCGW 1 cut(s) 1317
PctI GAATGC 1 cut(s) 321
PdiI GCCGGC 2 cut(s) 421, 594
PfeI GAWTC 1 cut(s) 43
PflFI GACNNNGTC 1 cut(s) 755
PflMI CCANNNNNTGG 1 cut(s) 704
PinAI ACCGGT 1 cut(s) 1141
PkrI GCNGC 7 cut(s) 85, 578, 607, 610, 790, 872, 1273
PleI GAGTC 2 cut(s) 90, 958
PpsI GAGTC 2 cut(s) 90, 958
Psp6I CCWGG 1 cut(s) 1396
PspEI GGTNACC 1 cut(s) 427
PspFI CCCAGC 2 cut(s) 446, 1411
PspGI CCWGG 1 cut(s) 1396
PspN4I GGNNCC 5 cut(s) 291, 1353, 1393, 1437, 1476
PspPI GGNCC 4 cut(s) 486, 498, 590, 761
PstI CTGCAG 1 cut(s) 88
PsyI GACNNNGTC 1 cut(s) 755
PvuII CAGCTG 2 cut(s) 83, 377
RsaI GTAC 2 cut(s) 857, 1437
RsaNI GTAC 2 cut(s) 856, 1436
RseI CAYNNNNRTG 2 cut(s) 230, 1044
SalI GTCGAC 1 cut(s) 1369
SaqAI TTAA 2 cut(s) 659, 1542
SatI GCNGC 7 cut(s) 84, 577, 606, 609, 789, 871, 1272
Sau3AI GATC 2 cut(s) 124, 1148
Sau96I GGNCC 4 cut(s) 486, 498, 590, 761
SchI GAGTC 2 cut(s) 90, 958
ScrFI CCNGG 4 cut(s) 239, 412, 413, 1398
SduI GDGCHC 1 cut(s) 411
SfaNI GCATC 6 cut(s) 232, 496, 699, 775, 857, 1455
SfcI CTRYAG 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 975
SgrAI CRCCGGYG 1 cut(s) 419
SinI GGWCC 2 cut(s) 486, 498
SmaI CCCGGG 1 cut(s) 413
SmiMI CAYNNNNRTG 2 cut(s) 230, 1044
SmlI CTYRAG 5 cut(s) 160, 215, 475, 580, 1121
SmoI CTYRAG 5 cut(s) 160, 215, 475, 580, 1121
Sse9I AATT 8 cut(s) 556, 702, 765, 881, 1024, 1060, 1101, 1485
SseBI AGGCCT 1 cut(s) 1221
SsiI CCGC 6 cut(s) 576, 588, 605, 789, 870, 1458
SspMI CTAG 3 cut(s) 134, 771, 1337
StuI AGGCCT 1 cut(s) 1221
StyD4I CCNGG 4 cut(s) 237, 410, 411, 1396
StyI CCWWGG 6 cut(s) 184, 354, 538, 864, 1152, 1216
TaaI ACNGT 5 cut(s) 625, 757, 778, 1366, 1440
TaiI ACGT 2 cut(s) 1164, 1376
TaqI TCGA 7 cut(s) 653, 718, 851, 975, 1147, 1311, 1370
TasI AATT 8 cut(s) 556, 702, 765, 881, 1024, 1060, 1101, 1485
TauI GCSGC 4 cut(s) 579, 608, 791, 873
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 2 cut(s) 659, 1542
Tru9I TTAA 2 cut(s) 659, 1542
TscAI CASTG 5 cut(s) 630, 636, 687, 955, 1350
TseFI GTSAC 6 cut(s) 63, 300, 427, 625, 950, 1321
TseI GCWGC 3 cut(s) 83, 608, 1271
Tsp45I GTSAC 6 cut(s) 63, 300, 427, 625, 950, 1321
TspDTI ATGAA 1 cut(s) 1062
TspMI CCCGGG 1 cut(s) 411
TspRI CASTG 5 cut(s) 630, 636, 687, 955, 1350
Tth111I GACNNNGTC 1 cut(s) 755
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 486, 498
XagI CCTNNNNNAGG 1 cut(s) 493
XapI RAATTY 1 cut(s) 1101
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XmaI CCCGGG 1 cut(s) 411
XmiI GTMKAC 2 cut(s) 1020, 1370
XspI CTAG 3 cut(s) 134, 771, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.