Rroxscaffold_7G00215570

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000427
Physical Location & Seq
Forward (+)
66198032 .. 66199754
1723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215570.1

Sequence Viewer

Length: 813 bp
ATGCGCTTCACCTTGCCGACAAGCGGTGACTACGAGCGCAGGACTAAGGCACCGGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACTAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCACTGCTTGGATCTGTCGTGCCTCGTAATGCTGGCGTGGTGGAGAAGTTGAGGAAAGCAGGGGCAATCATTTTGGGAAAGGCAAGCTTGAGCGAGTGGTCATATTTTAGGTGTAGTAAAGCACCCAATGGTTGGAGAGCCAGAGGCGGTCATGGCTTGAACCCTTACACATTCTCAGACCCTTGTCGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTGTCATTAGGTACTGAGACAGATGGTTCAATCTTATGTCCATCAGATTTCAACTCGGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCCAAGACAGGATACTGTTGGGTACGTAAGTGAAGATAACATATATCAATGCCGATTTATAATTAGTACAATGCCGACTAAATCAGGTTGCATTGTTTTCATCACTCATTTCTTGGGGTTGCGATCGAGCAGGCCAATGCGTAGGACAGTAGCAGATGCTGCTTATATTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAAGCGCGACGGACTTAGATCGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

270

Amino Acids

29.1

Weight (kDa)

9.82

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 20 - 177 1.2e-44 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 557
AccB1I GGYRCC 1 cut(s) 49
AccB7I CCANNNNNTGG 1 cut(s) 279
AccII CGCG 1 cut(s) 762
AciI CCGC 5 cut(s) 24, 135, 294, 345, 380
AclWI GGATC 1 cut(s) 166
AfaI GTAC 4 cut(s) 394, 521, 565, 725
AfiI CCNNNNNNNGG 4 cut(s) 23, 279, 461, 737
AgeI ACCGGT 1 cut(s) 52
AgsI TTSAA 3 cut(s) 307, 411, 433
AhlI ACTAGT 1 cut(s) 470
AluBI AGCT 1 cut(s) 234
AluI AGCT 1 cut(s) 234
Alw26I GTCTC 2 cut(s) 392, 707
AlwI GGATC 1 cut(s) 166
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 1 cut(s) 629
ApeKI GCWGC 4 cut(s) 342, 365, 377, 656
AsiGI ACCGGT 1 cut(s) 52
AspLEI GCGC 3 cut(s) 6, 39, 762
AsuHPI GGTGA 1 cut(s) 38
BaeI ACNNNNGTAYC 2 cut(s) 511, 544
BanI GGYRCC 1 cut(s) 49
BbvI GCAGC 4 cut(s) 354, 377, 389, 643
BccI CCATC 2 cut(s) 398, 430
BciVI GTATCC 1 cut(s) 502
BcoDI GTCTC 2 cut(s) 392, 707
BcuI ACTAGT 1 cut(s) 470
BfaI CTAG 1 cut(s) 471
BfuI GTATCC 1 cut(s) 502
BisI GCNGC 4 cut(s) 343, 366, 378, 657
BlsI GCNGC 4 cut(s) 344, 367, 379, 658
BmiI GGNNCC 1 cut(s) 51
BmsI GCATC 4 cut(s) 456, 643, 661, 693
BoxI GACNNNNGTC 1 cut(s) 330
BpuEI CTTGAG 3 cut(s) 256, 322, 740
BsaAI YACGTR 1 cut(s) 523
BsaWI WCCGGW 1 cut(s) 52
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 279, 461, 737
Bse118I RCCGGY 2 cut(s) 52, 137
Bse3DI GCAATG 2 cut(s) 105, 699
BseLI CCNNNNNNNGG 4 cut(s) 23, 279, 461, 737
BseMI GCAATG 2 cut(s) 105, 699
BseMII CTCAG 2 cut(s) 336, 387
BseXI GCAGC 4 cut(s) 354, 377, 389, 643
Bsh1236I CGCG 1 cut(s) 762
Bsh1285I CGRYCG 1 cut(s) 623
BshFI GGCC 1 cut(s) 631
BshNI GGYRCC 1 cut(s) 49
BshTI ACCGGT 1 cut(s) 52
BsiEI CGRYCG 1 cut(s) 623
BsiSI CCGG 2 cut(s) 53, 138
BslFI GGGAC 1 cut(s) 473
BslI CCNNNNNNNGG 4 cut(s) 23, 279, 461, 737
BsmAI GTCTC 2 cut(s) 392, 707
BsmFI GGGAC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 81
BsnI GGCC 1 cut(s) 631
Bsp143I GATC 3 cut(s) 158, 620, 773
BspACI CCGC 5 cut(s) 24, 135, 294, 345, 380
BspANI GGCC 1 cut(s) 631
BspCNI CTCAG 2 cut(s) 335, 388
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 1 cut(s) 51
BspPI GGATC 1 cut(s) 166
BspT107I GGYRCC 1 cut(s) 49
BsrDI GCAATG 2 cut(s) 105, 699
BsrFI RCCGGY 2 cut(s) 52, 137
BssAI RCCGGY 2 cut(s) 52, 137
BssMI GATC 3 cut(s) 158, 620, 773
Bst4CI ACNGT 3 cut(s) 514, 646, 750
BstAPI GCANNNNNTGC 1 cut(s) 656
BstBAI YACGTR 1 cut(s) 523
BstC8I GCNNGC 5 cut(s) 139, 181, 232, 629, 682
BstDEI CTNAG 6 cut(s) 45, 69, 114, 322, 396, 770
BstFNI CGCG 1 cut(s) 762
BstHHI GCGC 3 cut(s) 6, 39, 762
BstKTI GATC 3 cut(s) 161, 623, 776
BstMAI GTCTC 2 cut(s) 392, 707
BstMBI GATC 3 cut(s) 158, 620, 773
BstMCI CGRYCG 1 cut(s) 623
BstMWI GCNNNNNNNGC 4 cut(s) 300, 342, 374, 656
BstPAI GACNNNNGTC 1 cut(s) 330
BstSNI TACGTA 1 cut(s) 523
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 4 cut(s) 354, 377, 389, 643
BstX2I RGATCY 1 cut(s) 158
BstYI RGATCY 1 cut(s) 158
BsuI GTATCC 1 cut(s) 502
BsuRI GGCC 1 cut(s) 631
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 2 cut(s) 149, 366
Cac8I GCNNGC 5 cut(s) 139, 181, 232, 629, 682
CaiI CAGNNNCTG 1 cut(s) 656
CfoI GCGC 3 cut(s) 6, 39, 762
Cfr10I RCCGGY 2 cut(s) 52, 137
Csp6I GTAC 4 cut(s) 393, 520, 564, 724
CspAI ACCGGT 1 cut(s) 52
CspCI CAANNNNNGTGG 2 cut(s) 342, 377
CviAII CATG 2 cut(s) 77, 299
CviJI RGCY 6 cut(s) 141, 234, 287, 303, 631, 804
CviKI_1 RGCY 6 cut(s) 141, 234, 287, 303, 631, 804
CviQI GTAC 4 cut(s) 393, 520, 564, 724
DdeI CTNAG 6 cut(s) 45, 69, 114, 322, 396, 770
DpnI GATC 3 cut(s) 160, 622, 775
DpnII GATC 3 cut(s) 158, 620, 773
Eco105I TACGTA 1 cut(s) 523
FaeI CATG 2 cut(s) 80, 302
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 473
FatI CATG 2 cut(s) 76, 298
Fnu4HI GCNGC 4 cut(s) 343, 366, 378, 657
Fsp4HI GCNGC 4 cut(s) 343, 366, 378, 657
FspBI CTAG 1 cut(s) 471
GlaI GCGC 3 cut(s) 5, 38, 761
GluI GCNGC 4 cut(s) 343, 366, 378, 657
HaeIII GGCC 1 cut(s) 631
HapII CCGG 2 cut(s) 53, 138
HhaI GCGC 3 cut(s) 6, 39, 762
Hin1II CATG 2 cut(s) 80, 302
Hin6I GCGC 3 cut(s) 4, 37, 760
HinP1I GCGC 3 cut(s) 4, 37, 760
HindIII AAGCTT 1 cut(s) 232
HpaII CCGG 2 cut(s) 53, 138
HphI GGTGA 1 cut(s) 38
Hpy188I TCNGA 2 cut(s) 325, 427
Hpy188III TCNNGA 1 cut(s) 668
Hpy99I CGWCG 1 cut(s) 767
HpyCH4III ACNGT 3 cut(s) 514, 646, 750
HpyCH4IV ACGT 1 cut(s) 522
HpyCH4V TGCA 6 cut(s) 76, 110, 149, 588, 704, 746
HpyF10VI GCNNNNNNNGC 4 cut(s) 300, 342, 374, 656
HpyF3I CTNAG 6 cut(s) 45, 69, 114, 322, 396, 770
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 2 cut(s) 80, 302
HspAI GCGC 3 cut(s) 4, 37, 760
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Kzo9I GATC 3 cut(s) 158, 620, 773
Lsp1109I GCAGC 4 cut(s) 354, 377, 389, 643
LweI GCATC 4 cut(s) 456, 643, 661, 693
MaeI CTAG 1 cut(s) 471
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 2 cut(s) 26, 57
MalI GATC 3 cut(s) 160, 622, 775
MboI GATC 3 cut(s) 158, 620, 773
MboII GAAGA 1 cut(s) 542
MflI RGATCY 1 cut(s) 158
MluCI AATT 2 cut(s) 492, 558
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 4 cut(s) 180, 192, 284, 771
MroNI GCCGGC 1 cut(s) 137
MspA1I CMGCKG 2 cut(s) 345, 380
MspI CCGG 2 cut(s) 53, 138
Mva1269I GAATGC 1 cut(s) 81
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 4 cut(s) 300, 342, 374, 656
NaeI GCCGGC 1 cut(s) 139
NdeII GATC 3 cut(s) 158, 620, 773
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 2 cut(s) 80, 302
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 2 cut(s) 26, 57
PctI GAATGC 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 139
PflMI CCANNNNNTGG 1 cut(s) 279
PinAI ACCGGT 1 cut(s) 52
PkrI GCNGC 4 cut(s) 344, 367, 379, 658
Ple19I CGATCG 1 cut(s) 623
Ppu21I YACGTR 1 cut(s) 523
PshAI GACNNNNGTC 1 cut(s) 330
PsiI TTATAA 1 cut(s) 557
PspN4I GGNNCC 1 cut(s) 51
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 1 cut(s) 158
PvuI CGATCG 1 cut(s) 623
RsaI GTAC 4 cut(s) 394, 521, 565, 725
RsaNI GTAC 4 cut(s) 393, 520, 564, 724
SatI GCNGC 4 cut(s) 343, 366, 378, 657
Sau3AI GATC 3 cut(s) 158, 620, 773
SetI ASST 7 cut(s) 14, 236, 260, 394, 525, 586, 739
SfaNI GCATC 4 cut(s) 456, 643, 661, 693
SgrAI CRCCGGYG 1 cut(s) 52
SmlI CTYRAG 3 cut(s) 235, 337, 755
SmoI CTYRAG 3 cut(s) 235, 337, 755
SnaBI TACGTA 1 cut(s) 523
SpeI ACTAGT 1 cut(s) 470
Sse9I AATT 2 cut(s) 492, 558
SsiI CCGC 5 cut(s) 24, 135, 294, 345, 380
SspMI CTAG 1 cut(s) 471
TaaI ACNGT 3 cut(s) 514, 646, 750
TaiI ACGT 1 cut(s) 525
TaqI TCGA 4 cut(s) 475, 623, 687, 776
TaqII GACCGA 1 cut(s) 452
TasI AATT 2 cut(s) 492, 558
TatI WGTACW 2 cut(s) 563, 723
TscAI CASTG 2 cut(s) 156, 366
TseFI GTSAC 2 cut(s) 26, 57
TseI GCWGC 4 cut(s) 342, 365, 377, 656
Tsp45I GTSAC 2 cut(s) 26, 57
TspDTI ATGAA 2 cut(s) 140, 586
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 2 cut(s) 156, 366
Van91I CCANNNNNTGG 1 cut(s) 279
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.