Rmu_co8259027.1_g000001

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8259027.1
OG0000427
Physical Location & Seq
Forward (+)
1 .. 805
805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8259027.1_g000001.1.cds

Sequence Viewer

Length: 561 bp
cacttccttggctcaaacttttgagaaacatctcaacactctgaggaaaggaggagctgttttggtagacaatttggaaattgctaatttgactgaaatctattctagaagcgatgaatatgctgcattgtatattgagtgcaaaataaccttaaatgcatacttgaaggagttggtggcttccccggtgcgatccttggcagatgttatagccttcaacaagaaacacaagaaactggaaaagatcaaggagtatgggcaagacctatttttagcagctgaagcaacaaatgggtttggaaaaacagagaagctggcattgttgaatcttgcaaggtggacaagaaatggtcttgtgaaattggtgacaaagaagaagctagatgctgtggtgtctccttacgcaagtttgtcatgtgtacttgcaattgggggagccccaggtctaatagttccagctggatacgaaaacaatggggaaccagctggtatatgctttggtggacttcggggttcagagccaaagctaattgagattgcatatgcttttgagcaagccac
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

187

Amino Acids

20.28

Weight (kDa)

8.66

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 67
AclWI GGATC 1 cut(s) 187
AcuI CTGAAG 1 cut(s) 301
AfaI GTAC 1 cut(s) 421
AgsI TTSAA 3 cut(s) 167, 218, 326
AjnI CCWGG 1 cut(s) 440
AluBI AGCT 7 cut(s) 57, 279, 314, 380, 459, 486, 527
AluI AGCT 7 cut(s) 57, 279, 314, 380, 459, 486, 527
Alw26I GTCTC 1 cut(s) 400
AlwI GGATC 1 cut(s) 187
ApeKI GCWGC 2 cut(s) 123, 276
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 377
BanII GRGCYC 1 cut(s) 440
BbvI GCAGC 2 cut(s) 110, 288
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BciT130I CCWGG 1 cut(s) 442
BciVI GTATCC 1 cut(s) 456
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 2 cut(s) 106, 381
BfuI GTATCC 1 cut(s) 456
BisI GCNGC 2 cut(s) 124, 277
BlsI GCNGC 2 cut(s) 125, 278
Bme1390I CCNGG 2 cut(s) 186, 442
BmiI GGNNCC 2 cut(s) 437, 481
BmrFI CCNGG 2 cut(s) 186, 442
BmsI GCATC 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 7, 184, 196, 440
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bse1I ACTGG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 442
BseDI CCNNGG 4 cut(s) 7, 184, 196, 440
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 1 cut(s) 67
BseXI GCAGC 2 cut(s) 110, 288
BsiSI CCGG 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 400
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 2 cut(s) 192, 244
BspCNI CTCAG 1 cut(s) 34
BspLI GGNNCC 2 cut(s) 437, 481
BspPI GGATC 1 cut(s) 187
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 4 cut(s) 7, 184, 196, 440
BssMI GATC 2 cut(s) 192, 244
BssT1I CCWWGG 2 cut(s) 7, 196
Bst2UI CCWGG 1 cut(s) 442
BstC8I GCNNGC 2 cut(s) 316, 556
BstDEI CTNAG 1 cut(s) 42
BstKTI GATC 2 cut(s) 195, 247
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 2 cut(s) 192, 244
BstMWI GCNNNNNNNGC 1 cut(s) 282
BstNI CCWGG 1 cut(s) 442
BstSCI CCNGG 2 cut(s) 184, 440
BstV1I GCAGC 2 cut(s) 110, 288
BsuI GTATCC 1 cut(s) 456
BtgZI GCGATG 1 cut(s) 127
Cac8I GCNNGC 2 cut(s) 316, 556
Csp6I GTAC 1 cut(s) 420
CviAII CATG 1 cut(s) 415
CviQI GTAC 1 cut(s) 420
DdeI CTNAG 1 cut(s) 42
DpnI GATC 2 cut(s) 194, 246
DpnII GATC 2 cut(s) 192, 244
Eco130I CCWWGG 2 cut(s) 7, 196
Eco24I GRGCYC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 440
EcoT14I CCWWGG 2 cut(s) 7, 196
EcoT22I ATGCAT 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 440
ErhI CCWWGG 2 cut(s) 7, 196
FaeI CATG 1 cut(s) 418
FatI CATG 1 cut(s) 414
FauNDI CATATG 1 cut(s) 542
FblI GTMKAC 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 124, 277
FriOI GRGCYC 1 cut(s) 440
Fsp4HI GCNGC 2 cut(s) 124, 277
FspBI CTAG 2 cut(s) 106, 381
GluI GCNGC 2 cut(s) 124, 277
HapII CCGG 1 cut(s) 186
Hin1II CATG 1 cut(s) 418
HinfI GANTC 1 cut(s) 326
HpaII CCGG 1 cut(s) 186
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 4 cut(s) 68, 340, 420, 504
Hpy188I TCNGA 2 cut(s) 43, 518
Hpy188III TCNNGA 1 cut(s) 106
Hpy8I GTNNAC 4 cut(s) 68, 340, 420, 504
HpyAV CCTTC 2 cut(s) 161, 224
HpyCH4V TGCA 6 cut(s) 126, 142, 159, 333, 426, 540
HpyF10VI GCNNNNNNNGC 1 cut(s) 282
HpyF3I CTNAG 1 cut(s) 42
Hsp92II CATG 1 cut(s) 418
Kzo9I GATC 2 cut(s) 192, 244
LmnI GCTCC 2 cut(s) 54, 435
LpnPI CCDG 9 cut(s) 199, 222, 300, 427, 445, 454, 469, 472, 496
Lsp1109I GCAGC 2 cut(s) 110, 288
LweI GCATC 1 cut(s) 374
MaeI CTAG 2 cut(s) 106, 381
MaeIII GTNAC 1 cut(s) 365
MalI GATC 2 cut(s) 194, 246
MboI GATC 2 cut(s) 192, 244
MboII GAAGA 1 cut(s) 386
MfeI CAATTG 1 cut(s) 427
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 6 cut(s) 71, 79, 86, 360, 427, 529
MnlI CCTC 2 cut(s) 37, 45
Mph1103I ATGCAT 1 cut(s) 161
MseI TTAA 1 cut(s) 153
MspA1I CMGCKG 3 cut(s) 279, 459, 486
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 2 cut(s) 186, 442
MunI CAATTG 1 cut(s) 427
MvaI CCWGG 1 cut(s) 442
MwoI GCNNNNNNNGC 1 cut(s) 282
NciI CCSGG 1 cut(s) 186
NdeI CATATG 1 cut(s) 542
NdeII GATC 2 cut(s) 192, 244
NlaIII CATG 1 cut(s) 418
NlaIV GGNNCC 2 cut(s) 437, 481
NmuCI GTSAC 1 cut(s) 365
NsiI ATGCAT 1 cut(s) 161
PfeI GAWTC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 125, 278
Psp6I CCWGG 1 cut(s) 440
PspGI CCWGG 1 cut(s) 440
PspN4I GGNNCC 2 cut(s) 437, 481
PvuII CAGCTG 3 cut(s) 279, 459, 486
RsaI GTAC 1 cut(s) 421
RsaNI GTAC 1 cut(s) 420
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 2 cut(s) 124, 277
Sau3AI GATC 2 cut(s) 192, 244
ScrFI CCNGG 2 cut(s) 186, 442
SduI GDGCHC 1 cut(s) 440
SfaNI GCATC 1 cut(s) 374
Sse9I AATT 6 cut(s) 71, 79, 86, 360, 427, 529
SspMI CTAG 2 cut(s) 106, 381
StyD4I CCNGG 2 cut(s) 184, 440
StyI CCWWGG 2 cut(s) 7, 196
TasI AATT 6 cut(s) 71, 79, 86, 360, 427, 529
TatI WGTACW 1 cut(s) 419
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 2 cut(s) 123, 276
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 1 cut(s) 130
XbaI TCTAGA 1 cut(s) 105
XmiI GTMKAC 1 cut(s) 67
XspI CTAG 2 cut(s) 106, 381
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.