pycom05g06760

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000427
Physical Location & Seq
Forward (+)
9475536 .. 9478097
2562 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g06760.1

Sequence Viewer

Length: 1722 bp
ATGGCTACCACCAATTCAAGGCAGAGACTTTTCTCTCTCCTCTCGTTTCTTATTCTAATTCTTCAATCCAAAATCTCATCTGGGTCCGAAACCCCAATTGAAGAATCCACCATTGACGACCTCCAGATCGCTTTCAAGCTCAAGACTCTGACTTCACGGCAACTGGTTCAGTTCTACCTGGACAGAATCCAAACCCTCAACAGCCACCTTAGAGGGGTCCTGGAGGTGAACCCAGATGCGCTTTCCCAAGCCGATTCCGCCGACGAACAACGCCGGAAATGGGAATCAAACCCATCAGCCAGAACCAGAGCACTGCCGAAGCTGCACGGGATTCCCATTTTGCTCAAGGACAACATTGGCACCAAGGACAAACTTAACACCACCGTCGGCTCCTTTGCGCTGTTGGGTTCCAAAGTTCCGAGGGATGCCGGGGTTGTGGCGAAGCTGAGGAGCTCTGGGGCTGTGATTCTGGGGAAGGGTAGCTTGAGCGAGTGGTCGTATATCCGGTCGTTTTCCGCGCCGTACGGATGGAGTGCCAGAGGCGGCCAGGGTGTGAATCCTTATAATTCATCGCTGGAAGTTTGTGGATCAAGTAGTGGACCAGCAATATCTGTATCTGCAAATATGGTAGCTGTGGCTTTAGGGACAGAGACTGACAGCTCCATCTTATGTCCGTCCAGTTATAACTCGGTAGTAGGCTTCAAGCCAACCGTTGGTCTCACTAGTCGAGCTGGAGTCATCCCAGTGAGTCCGAGACAGGACACAGTTGGGCCAATTTGTAGAACAGTGGCTGATGCTGTTCATGTCCTGGATGCCATTGCCGGTATTGATAGCAACGACATCGCAACGCATGAAGCATCGCAGTACATCCCAGTGGGTGGCTATGGACAGTTTCTCAAGGCTAATGGTCTCAAAGGAAAGAGATTGGGGATAGTAAGTCGCTTGTTTCGTGAAGTCAAGGATGATTGTTTCTTGAATCAAACTTTTGAGCAGCACTTTACCACACTGAGGAAACAAGGTGCAGTTTTGGTTGGAAATCTTGAGATAGCCAACATTGAAGATATTCTCGATTATTCTTTGAGTGGTGAATTTGTAGCAATGTTAGCGGAATTCAAAATGGCCTTGAACCCATACCTAAGTGAACTAGTTGAATCTCCGGTGAGAACCTTGAGAGATGTGATAGCATTCAACAAGAACAACTCAGTTTTGGAAAAGATGAATGAATATGGACAAGATCTCTTAGAAGCTGCTGAGAAGACTAACGGTATAGGAGCAAAAGAGCAAGAAATATTGTCAAATTTAGCAAGACTGTCCAGAGATGGATTCGAGAAGTTGATGAAAGATGAGAACCTAGACGCGTTGGTGACTTATTCCAACTCTGCTCATTCAATCCTTGGAATTGGTGGTTTTCCAGGCATTGTTGTGCCGGCAGGATATCATAACGATGACAAGTACCCTTTCGGCATTTGCTTTGGGGGACTAAAGGGTTCGGAGCCAAAGCTGATTGAGGTTGCGTATGCATTTGAGCAAGCAACTAAGATCAGAAAGCCTCCGCCCGTTGGTTCTCAAGGCCAACTCCTCTCTACCACCGCTACTCCCACTCCTGAAGTTGAGTCATCAATACCCACCATTGCTGCAGCCACAAGAAGACCCGTCCTAGTCACTCAAGAGAACCTCCAAGGAAAGCGCTTGCATGACAGCAGTCGACATGAAGTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

574

Amino Acids

61.91

Weight (kDa)

6.78

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 55 - 456 4.7e-66 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 564, 684
AccB1I GGYRCC 1 cut(s) 359
AccB7I CCANNNNNTGG 2 cut(s) 713, 878
AccI GTMKAC 1 cut(s) 1705
AccII CGCG 2 cut(s) 518, 1358
AciI CCGC 6 cut(s) 258, 516, 543, 1106, 1553, 1590
AclWI GGATC 1 cut(s) 595
AcoI YGGCCR 1 cut(s) 544
AcsI RAATTY 3 cut(s) 1088, 1109, 1297
AcuI CTGAAG 1 cut(s) 1626
AfaI GTAC 3 cut(s) 524, 866, 1454
AfeI AGCGCT 1 cut(s) 1688
AfiI CCNNNNNNNGG 7 cut(s) 18, 214, 280, 713, 878, 1008, 1559
AflIII ACRYGT 1 cut(s) 1356
AhlI ACTAGT 2 cut(s) 722, 1144
AjnI CCWGG 5 cut(s) 177, 219, 546, 807, 1411
Alw21I GWGCWC 2 cut(s) 313, 455
Alw26I GTCTC 5 cut(s) 19, 644, 722, 748, 914
AlwI GGATC 1 cut(s) 595
AlwNI CAGNNNCTG 2 cut(s) 653, 791
Aor51HI AGCGCT 1 cut(s) 1688
AoxI GGCC 4 cut(s) 544, 770, 1119, 1570
ApeKI GCWGC 5 cut(s) 322, 991, 1247, 1634, 1637
ApoI RAATTY 3 cut(s) 1088, 1109, 1297
Asp700I GAANNNNTTC 1 cut(s) 1062
AspLEI GCGC 4 cut(s) 241, 400, 520, 1689
AspS9I GGNCC 4 cut(s) 84, 217, 599, 770
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 4 cut(s) 238, 1097, 1171, 1375
AvaII GGWCC 3 cut(s) 84, 217, 599
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 1 cut(s) 455
BbsI GAAGAC 2 cut(s) 1262, 1654
Bbv12I GWGCWC 2 cut(s) 313, 455
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 5 cut(s) 309, 1003, 1234, 1621, 1649
BccI CCATC 4 cut(s) 301, 522, 671, 1313
BceAI ACGGC 2 cut(s) 173, 505
BcgI CGANNNNNNTGC 2 cut(s) 823, 857
BciT130I CCWGG 5 cut(s) 179, 221, 548, 809, 1413
BcnI CCSGG 1 cut(s) 430
BcoDI GTCTC 5 cut(s) 19, 644, 722, 748, 914
BcuI ACTAGT 2 cut(s) 722, 1144
BfaI CTAG 5 cut(s) 723, 1145, 1352, 1658, 1720
BfmI CTRYAG 1 cut(s) 1635
BfoI RGCGCY 1 cut(s) 1690
BglII AGATCT 1 cut(s) 1234
BisI GCNGC 6 cut(s) 323, 544, 992, 1248, 1635, 1638
BlsI GCNGC 6 cut(s) 324, 545, 993, 1249, 1636, 1639
Bme1390I CCNGG 6 cut(s) 179, 221, 430, 548, 809, 1413
Bme18I GGWCC 3 cut(s) 84, 217, 599
BmgT120I GGNCC 4 cut(s) 84, 217, 599, 770
BmiI GGNNCC 6 cut(s) 85, 218, 361, 391, 409, 1494
BmrFI CCNGG 6 cut(s) 179, 221, 430, 548, 809, 1413
BmrI ACTGGG 2 cut(s) 737, 866
BmsI GCATC 5 cut(s) 226, 415, 784, 802, 866
BmuI ACTGGG 2 cut(s) 737, 866
BoxI GACNNNNGTC 1 cut(s) 1701
BpiI GAAGAC 2 cut(s) 1262, 1654
BpmI CTGGAG 3 cut(s) 107, 242, 753
Bpu10I CCTNAGC 1 cut(s) 446
BpuEI CTTGAG 8 cut(s) 125, 329, 505, 881, 1061, 1189, 1551, 1650
BpuMI CCSGG 1 cut(s) 430
BsaI GGTCTC 2 cut(s) 722, 914
BsaJI CCNNGG 6 cut(s) 363, 419, 429, 547, 1393, 1678
BsaWI WCCGGW 2 cut(s) 504, 1156
BsaXI ACNNNNNCTCC 4 cut(s) 1579, 1585, 1609, 1615
Bsc4I CCNNNNNNNGG 7 cut(s) 18, 214, 280, 713, 878, 1008, 1559
Bse118I RCCGGY 2 cut(s) 821, 1426
Bse1I ACTGG 4 cut(s) 168, 678, 743, 872
Bse3DI GCAATG 3 cut(s) 816, 1104, 1629
BseBI CCWGG 5 cut(s) 179, 221, 548, 809, 1413
BseDI CCNNGG 6 cut(s) 363, 419, 429, 547, 1393, 1678
BseGI GGATG 6 cut(s) 430, 533, 738, 817, 867, 967
BseLI CCNNNNNNNGG 7 cut(s) 18, 214, 280, 713, 878, 1008, 1559
BseMI GCAATG 3 cut(s) 816, 1104, 1629
BseMII CTCAG 4 cut(s) 437, 998, 1215, 1242
BseNI ACTGG 4 cut(s) 168, 678, 743, 872
BseRI GAGGAG 3 cut(s) 29, 463, 1568
BseXI GCAGC 5 cut(s) 309, 1003, 1234, 1621, 1649
BsgI GTGCAG 2 cut(s) 308, 1041
Bsh1236I CGCG 2 cut(s) 518, 1358
Bsh1285I CGRYCG 1 cut(s) 509
BshFI GGCC 4 cut(s) 546, 772, 1121, 1572
BshNI GGYRCC 1 cut(s) 359
BsiEI CGRYCG 1 cut(s) 509
BsiHKAI GWGCWC 2 cut(s) 313, 455
BsiSI CCGG 6 cut(s) 274, 429, 505, 822, 1157, 1427
BsiWI CGTACG 1 cut(s) 522
BslFI GGGAC 2 cut(s) 658, 1491
BslI CCNNNNNNNGG 7 cut(s) 18, 214, 280, 713, 878, 1008, 1559
BsmAI GTCTC 5 cut(s) 19, 644, 722, 748, 914
BsmFI GGGAC 2 cut(s) 658, 1491
BsmI GAATGC 1 cut(s) 1184
BsnI GGCC 4 cut(s) 546, 772, 1121, 1572
Bso31I GGTCTC 2 cut(s) 722, 914
Bsp1286I GDGCHC 2 cut(s) 313, 455
Bsp143I GATC 4 cut(s) 126, 587, 1234, 1539
BspACI CCGC 6 cut(s) 258, 516, 543, 1106, 1553, 1590
BspANI GGCC 4 cut(s) 546, 772, 1121, 1572
BspCNI CTCAG 4 cut(s) 438, 999, 1214, 1243
BspFNI CGCG 2 cut(s) 518, 1358
BspLI GGNNCC 6 cut(s) 85, 218, 361, 391, 409, 1494
BspMAI CTGCAG 1 cut(s) 1639
BspPI GGATC 1 cut(s) 595
BspT107I GGYRCC 1 cut(s) 359
BspTNI GGTCTC 2 cut(s) 722, 914
BsrDI GCAATG 3 cut(s) 816, 1104, 1629
BsrFI RCCGGY 2 cut(s) 821, 1426
BsrI ACTGG 4 cut(s) 168, 678, 743, 872
BssAI RCCGGY 2 cut(s) 821, 1426
BssECI CCNNGG 6 cut(s) 363, 419, 429, 547, 1393, 1678
BssMI GATC 4 cut(s) 126, 587, 1234, 1539
BssT1I CCWWGG 3 cut(s) 363, 1393, 1678
Bst2UI CCWGG 5 cut(s) 179, 221, 548, 809, 1413
Bst4CI ACNGT 7 cut(s) 385, 712, 766, 787, 891, 1265, 1311
BstC8I GCNNGC 3 cut(s) 1428, 1530, 1691
BstDEI CTNAG 8 cut(s) 209, 446, 1007, 1136, 1201, 1240, 1251, 1536
BstF5I GGATG 6 cut(s) 430, 533, 738, 817, 867, 967
BstFNI CGCG 2 cut(s) 518, 1358
BstH2I RGCGCY 1 cut(s) 1690
BstHHI GCGC 4 cut(s) 241, 400, 520, 1689
BstKTI GATC 4 cut(s) 129, 590, 1237, 1542
BstMAI GTCTC 5 cut(s) 19, 644, 722, 748, 914
BstMBI GATC 4 cut(s) 126, 587, 1234, 1539
BstMCI CGRYCG 1 cut(s) 509
BstMWI GCNNNNNNNGC 3 cut(s) 257, 322, 1103
BstNI CCWGG 5 cut(s) 179, 221, 548, 809, 1413
BstPAI GACNNNNGTC 1 cut(s) 1701
BstSCI CCNGG 6 cut(s) 177, 219, 428, 546, 807, 1411
BstSFI CTRYAG 1 cut(s) 1635
BstUI CGCG 2 cut(s) 518, 1358
BstV1I GCAGC 5 cut(s) 309, 1003, 1234, 1621, 1649
BstV2I GAAGAC 2 cut(s) 1262, 1654
BstX2I RGATCY 1 cut(s) 1234
BstYI RGATCY 1 cut(s) 1234
BsuRI GGCC 4 cut(s) 546, 772, 1121, 1572
BtgZI GCGATG 3 cut(s) 555, 826, 843
BtsCI GGATG 6 cut(s) 430, 533, 738, 817, 867, 967
BtsI GCAGTG 1 cut(s) 311
BtsIMutI CAGTG 5 cut(s) 311, 750, 792, 879, 1004
Cac8I GCNNGC 3 cut(s) 1428, 1530, 1691
CaiI CAGNNNCTG 2 cut(s) 653, 791
CfoI GCGC 4 cut(s) 241, 400, 520, 1689
Cfr10I RCCGGY 2 cut(s) 821, 1426
Cfr13I GGNCC 4 cut(s) 84, 217, 599, 770
CseI GACGC 1 cut(s) 1364
Csp6I GTAC 3 cut(s) 523, 865, 1453
CviAII CATG 4 cut(s) 803, 851, 1694, 1709
CviQI GTAC 3 cut(s) 523, 865, 1453
DdeI CTNAG 8 cut(s) 209, 446, 1007, 1136, 1201, 1240, 1251, 1536
DpnI GATC 4 cut(s) 128, 589, 1236, 1541
DpnII GATC 4 cut(s) 126, 587, 1234, 1539
EaeI YGGCCR 1 cut(s) 544
EciI GGCGGA 2 cut(s) 247, 1542
Ecl136II GAGCTC 1 cut(s) 453
Eco130I CCWWGG 3 cut(s) 363, 1393, 1678
Eco24I GRGCYC 1 cut(s) 455
Eco31I GGTCTC 2 cut(s) 722, 914
Eco32I GATATC 1 cut(s) 1436
Eco47I GGWCC 3 cut(s) 84, 217, 599
Eco47III AGCGCT 1 cut(s) 1688
Eco53kI GAGCTC 1 cut(s) 453
Eco57I CTGAAG 1 cut(s) 1626
EcoICRI GAGCTC 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 1109
EcoRII CCWGG 5 cut(s) 177, 219, 546, 807, 1411
EcoRV GATATC 1 cut(s) 1436
EcoT14I CCWWGG 3 cut(s) 363, 1393, 1678
EcoT22I ATGCAT 1 cut(s) 1522
EcoT38I GRGCYC 1 cut(s) 455
ErhI CCWWGG 3 cut(s) 363, 1393, 1678
FaeI CATG 4 cut(s) 806, 854, 1697, 1712
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 2 cut(s) 658, 1491
FatI CATG 4 cut(s) 802, 850, 1693, 1708
FblI GTMKAC 1 cut(s) 1705
Fnu4HI GCNGC 6 cut(s) 323, 544, 992, 1248, 1635, 1638
FokI GGATG 6 cut(s) 437, 540, 725, 824, 854, 974
FriOI GRGCYC 1 cut(s) 455
Fsp4HI GCNGC 6 cut(s) 323, 544, 992, 1248, 1635, 1638
FspBI CTAG 5 cut(s) 723, 1145, 1352, 1658, 1720
GlaI GCGC 4 cut(s) 240, 399, 519, 1688
GluI GCNGC 6 cut(s) 323, 544, 992, 1248, 1635, 1638
GsuI CTGGAG 3 cut(s) 107, 242, 753
HaeII RGCGCY 1 cut(s) 1690
HaeIII GGCC 4 cut(s) 546, 772, 1121, 1572
HapII CCGG 6 cut(s) 274, 429, 505, 822, 1157, 1427
HgaI GACGC 1 cut(s) 1364
HhaI GCGC 4 cut(s) 241, 400, 520, 1689
Hin1II CATG 4 cut(s) 806, 854, 1697, 1712
Hin6I GCGC 4 cut(s) 239, 398, 518, 1687
HinP1I GCGC 4 cut(s) 239, 398, 518, 1687
HincII GTYRAC 1 cut(s) 1706
HindII GTYRAC 1 cut(s) 1706
HpaII CCGG 6 cut(s) 274, 429, 505, 822, 1157, 1427
HphI GGTGA 4 cut(s) 238, 1097, 1171, 1375
Hpy166II GTNNAC 4 cut(s) 229, 599, 1142, 1706
Hpy188I TCNGA 6 cut(s) 88, 150, 420, 753, 1492, 1544
Hpy8I GTNNAC 4 cut(s) 229, 599, 1142, 1706
Hpy99I CGWCG 2 cut(s) 266, 389
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 7 cut(s) 385, 712, 766, 787, 891, 1265, 1311
HpyCH4V TGCA 6 cut(s) 325, 620, 1022, 1520, 1637, 1693
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 322, 1103
HpyF3I CTNAG 8 cut(s) 209, 446, 1007, 1136, 1201, 1240, 1251, 1536
Hsp92II CATG 4 cut(s) 806, 854, 1697, 1712
HspAI GCGC 4 cut(s) 239, 398, 518, 1687
KroI GCCGGC 1 cut(s) 1426
KroNI GCCGGC 1 cut(s) 1428
Kzo9I GATC 4 cut(s) 126, 587, 1234, 1539
LmnI GCTCC 5 cut(s) 395, 450, 665, 1271, 1492
Lsp1109I GCAGC 5 cut(s) 309, 1003, 1234, 1621, 1649
LweI GCATC 5 cut(s) 226, 415, 784, 802, 866
MaeI CTAG 5 cut(s) 723, 1145, 1352, 1658, 1720
MaeIII GTNAC 2 cut(s) 1363, 1660
MalI GATC 4 cut(s) 128, 589, 1236, 1541
MboI GATC 4 cut(s) 126, 587, 1234, 1539
MboII GAAGA 5 cut(s) 53, 113, 1070, 1267, 1659
MfeI CAATTG 1 cut(s) 96
MflI RGATCY 1 cut(s) 1234
MhlI GDGCHC 2 cut(s) 313, 455
MluCI AATT 9 cut(s) 13, 57, 96, 565, 774, 1088, 1109, 1297, 1398
MluI ACGCGT 1 cut(s) 1356
MlyI GAGTC 4 cut(s) 139, 744, 757, 1622
MmeI TCCRAC 2 cut(s) 1012, 1398
Mph1103I ATGCAT 1 cut(s) 1522
MroNI GCCGGC 1 cut(s) 1426
MroXI GAANNNNTTC 1 cut(s) 1062
MseI TTAA 1 cut(s) 375
MslI CAYNNNNRTG 4 cut(s) 743, 872, 1421, 1443
MspI CCGG 6 cut(s) 274, 429, 505, 822, 1157, 1427
MspR9I CCNGG 6 cut(s) 179, 221, 430, 548, 809, 1413
MunI CAATTG 1 cut(s) 96
Mva1269I GAATGC 1 cut(s) 1184
MvaI CCWGG 5 cut(s) 179, 221, 548, 809, 1413
MvnI CGCG 2 cut(s) 518, 1358
MwoI GCNNNNNNNGC 3 cut(s) 257, 322, 1103
NaeI GCCGGC 1 cut(s) 1428
NciI CCSGG 1 cut(s) 430
NdeII GATC 4 cut(s) 126, 587, 1234, 1539
NgoMIV GCCGGC 1 cut(s) 1426
NlaIII CATG 4 cut(s) 806, 854, 1697, 1712
NlaIV GGNNCC 6 cut(s) 85, 218, 361, 391, 409, 1494
NmuCI GTSAC 2 cut(s) 1363, 1660
NsiI ATGCAT 1 cut(s) 1522
PcsI WCGNNNNNNNCGW 1 cut(s) 946
PctI GAATGC 1 cut(s) 1184
PdiI GCCGGC 1 cut(s) 1428
PdmI GAANNNNTTC 1 cut(s) 1062
Pfl23II CGTACG 1 cut(s) 522
PflMI CCANNNNNTGG 2 cut(s) 713, 878
PfoI TCCNGGA 2 cut(s) 219, 807
PkrI GCNGC 6 cut(s) 324, 545, 993, 1249, 1636, 1639
PleI GAGTC 4 cut(s) 139, 743, 756, 1621
PpsI GAGTC 4 cut(s) 139, 743, 756, 1621
PpuMI RGGWCCY 1 cut(s) 217
PshAI GACNNNNGTC 1 cut(s) 1701
PsiI TTATAA 2 cut(s) 564, 684
Psp124BI GAGCTC 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 5 cut(s) 177, 219, 546, 807, 1411
PspGI CCWGG 5 cut(s) 177, 219, 546, 807, 1411
PspLI CGTACG 1 cut(s) 522
PspN4I GGNNCC 6 cut(s) 85, 218, 361, 391, 409, 1494
PspPI GGNCC 4 cut(s) 84, 217, 599, 770
PspPPI RGGWCCY 1 cut(s) 217
PstI CTGCAG 1 cut(s) 1639
PstNI CAGNNNCTG 2 cut(s) 653, 791
PsuI RGATCY 1 cut(s) 1234
RsaI GTAC 3 cut(s) 524, 866, 1454
RsaNI GTAC 3 cut(s) 523, 865, 1453
RseI CAYNNNNRTG 4 cut(s) 743, 872, 1421, 1443
SacI GAGCTC 1 cut(s) 455
SalI GTCGAC 1 cut(s) 1704
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 6 cut(s) 323, 544, 992, 1248, 1635, 1638
Sau3AI GATC 4 cut(s) 126, 587, 1234, 1539
Sau96I GGNCC 4 cut(s) 84, 217, 599, 770
SchI GAGTC 4 cut(s) 139, 744, 757, 1622
ScrFI CCNGG 6 cut(s) 179, 221, 430, 548, 809, 1413
SduI GDGCHC 2 cut(s) 313, 455
SfaNI GCATC 5 cut(s) 226, 415, 784, 802, 866
SfcI CTRYAG 1 cut(s) 1635
SinI GGWCC 3 cut(s) 84, 217, 599
SmiMI CAYNNNNRTG 4 cut(s) 743, 872, 1421, 1443
SmlI CTYRAG 8 cut(s) 140, 344, 484, 896, 1040, 1168, 1566, 1665
SmoI CTYRAG 8 cut(s) 140, 344, 484, 896, 1040, 1168, 1566, 1665
SpeI ACTAGT 2 cut(s) 722, 1144
Sse9I AATT 9 cut(s) 13, 57, 96, 565, 774, 1088, 1109, 1297, 1398
SsiI CCGC 6 cut(s) 258, 516, 543, 1106, 1553, 1590
SspI AATATT 1 cut(s) 1290
SspMI CTAG 5 cut(s) 723, 1145, 1352, 1658, 1720
SstI GAGCTC 1 cut(s) 455
StyD4I CCNGG 6 cut(s) 177, 219, 428, 546, 807, 1411
StyI CCWWGG 3 cut(s) 363, 1393, 1678
TaaI ACNGT 7 cut(s) 385, 712, 766, 787, 891, 1265, 1311
TaqI TCGA 4 cut(s) 727, 1068, 1326, 1705
TasI AATT 9 cut(s) 13, 57, 96, 565, 774, 1088, 1109, 1297, 1398
TatI WGTACW 1 cut(s) 864
TauI GCSGC 1 cut(s) 546
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 5 cut(s) 318, 750, 792, 879, 1011
TseFI GTSAC 2 cut(s) 1363, 1660
TseI GCWGC 5 cut(s) 322, 991, 1247, 1634, 1637
Tsp45I GTSAC 2 cut(s) 1363, 1660
TspDTI ATGAA 6 cut(s) 558, 791, 867, 1232, 1236, 1352
TspGWI ACGGA 2 cut(s) 540, 663
TspRI CASTG 5 cut(s) 318, 750, 792, 879, 1011
Van91I CCANNNNNTGG 2 cut(s) 713, 878
VpaK11BI GGWCC 3 cut(s) 84, 217, 599
XapI RAATTY 3 cut(s) 1088, 1109, 1297
XmiI GTMKAC 1 cut(s) 1705
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 5 cut(s) 723, 1145, 1352, 1658, 1720
Zsp2I ATGCAT 1 cut(s) 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.