Rh6CG018800

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000427
Physical Location & Seq
Reverse (-)
1859535 .. 1862417
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG018800.1

Sequence Viewer

Length: 1344 bp
ATGTCTAAATCTTATAACCAACTAACTTCCAGACAAGTAGTTCAGTTCTACCTTCAAGAAATCCTCAGGCTCAACCCAGTTCTTAACGGAGTCATAGAGGTGAACCCGGATGCACTTTACCAAGCTGACAAGGCTGACTATCAGCGAAAGGCTAAGGCCCCTGGTTATTATTCCGGCTTGCATGGCATTCCTATTTTGCTCAAGGATAACATTGGAACCAGAGATAAGCTGAACACTACTGCTGGCTCTTTTGCGCTGCTCGGCTCTGTCGTGCCCCGGGATGCTGGCGTGGTGACCAAGTTGAGGAGCGCTGGAGTCATTATCTTGGGGAAGGCTAGCTTCAGTGAGTGGGCTCACGTTAGGTCTCTCACGTCACCCCCAGGTTTCAGCCCCAGAGGTGGCCAAGGAAAGAACCCTTATGTACTATCTGCAAGTCCATGTGGATCAAGCAGTGGATCATCAATATCAGTTGCAGCAAATTTGGTAGCTGTATCTCTTGCCGAACCTGGGCGATCCCGGCCAGTTACTCCCAGACAAGACACCATTGGACCCATCTGCAGGACTGTGACAGATGCTGTTTACGTTCTTGATGCGATTGTAGGCTATGACAACAAGGATCAAGCAACCAAAGAAGCATCGAAATACATTCCACGCGGTGGGTATAAGCAGTTCCTTGGGGCTTATGGGCTCAAGGGAAAGAGACTGGGAATAGTGAGGAATCCCTTCTTCACCTCTGGGAGTGGATCTCTCCAAATTCAAGCATTTGAGCATCATTTCCAAACGCTAAGGCAAGGAGGTGCTGTTTTGGTAGACCATCTGGAAATAGCAAACATTGATACTATCTTGAATTTTAATTTGAGTGGAGAAGCAATAGCAACAGTGGCTGAGTTCAAACTAGCCATAAATGCATATCTAAAAGACCTTGTGGTGTCCCCAGTTCGATCTTTGGCAGATATCATAGCCTTCAACTTGAAGTTTTCTGGTGTGGAAATGATCAAGGAATTTGGCCAAGACATTTTTCTTGCAGCACAAGCCACAAATGGGATAGGCAACAATGAGAAGGCAGCATTGTCATATTTAGCAAAGCTGACAAAAGATGGATTTGAGAAACTAATGACATACAACAGGCTAGATGCATTGGTGACTCCCGATGCAGATATTGCTCCTGTACTTGCACTTGGGGGATTCCCAGGCATTAGTGTCCCTGCTGGATATGACAAGAAGGGTGTGCCTTTCGGCATAACTTTTGGGGGCCTAAAGGGATCTGAACCAAAGCTTATACAGATTGCCTACGGTTTCGAGCAAGCTACTAAGATTAGAAAGCCTCCCACATTTCTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

447

Amino Acids

47.84

Weight (kDa)

9.42

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 13 - 166 2.5e-33 Amidase
Amidase PF01425 175 - 389 4.5e-08 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 15
AccB7I CCANNNNNTGG 1 cut(s) 656
AccI GTMKAC 1 cut(s) 810
AccII CGCG 1 cut(s) 654
AciI CCGC 1 cut(s) 654
AclWI GGATC 6 cut(s) 451, 463, 507, 624, 751, 1270
AcoI YGGCCR 3 cut(s) 400, 518, 1006
AcsI RAATTY 4 cut(s) 478, 753, 847, 1001
AcuI CTGAAG 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 656
AfaI GTAC 2 cut(s) 423, 1170
AfeI AGCGCT 1 cut(s) 310
AfiI CCNNNNNNNGG 6 cut(s) 303, 398, 507, 558, 656, 1041
AgsI TTSAA 6 cut(s) 56, 758, 847, 892, 967, 973
AjiI CACGTC 1 cut(s) 372
AjnI CCWGG 4 cut(s) 160, 379, 505, 1189
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
AluBI AGCT 7 cut(s) 125, 229, 339, 488, 1087, 1276, 1307
AluI AGCT 7 cut(s) 125, 229, 339, 488, 1087, 1276, 1307
Alw26I GTCTC 2 cut(s) 369, 694
AlwI GGATC 6 cut(s) 451, 463, 507, 624, 751, 1270
AlwNI CAGNNNCTG 2 cut(s) 575, 884
Ama87I CYCGRG 1 cut(s) 276
Aor51HI AGCGCT 1 cut(s) 310
AoxI GGCC 5 cut(s) 156, 400, 518, 1006, 1252
ApeKI GCWGC 4 cut(s) 256, 473, 1025, 1064
ApoI RAATTY 4 cut(s) 478, 753, 847, 1001
Asp700I GAANNNNTTC 1 cut(s) 722
AspLEI GCGC 2 cut(s) 256, 311
AspS9I GGNCC 3 cut(s) 157, 548, 1252
AsuC2I CCSGG 4 cut(s) 107, 277, 278, 517
AsuHPI GGTGA 5 cut(s) 112, 304, 366, 721, 1153
AsuNHI GCTAGC 1 cut(s) 335
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 1 cut(s) 548
AxyI CCTNAGG 1 cut(s) 65
BaeGI GKGCMC 1 cut(s) 276
BalI TGGCCA 2 cut(s) 402, 1008
BanII GRGCYC 2 cut(s) 355, 690
BbvI GCAGC 4 cut(s) 243, 485, 1037, 1076
BccI CCATC 3 cut(s) 560, 822, 1091
BciT130I CCWGG 4 cut(s) 162, 381, 507, 1191
BclI TGATCA 1 cut(s) 993
BcnI CCSGG 4 cut(s) 107, 277, 278, 517
BcoDI GTCTC 2 cut(s) 369, 694
BfaI CTAG 3 cut(s) 336, 896, 1130
BfmI CTRYAG 1 cut(s) 556
BfoI RGCGCY 1 cut(s) 312
BisI GCNGC 4 cut(s) 257, 474, 1026, 1065
BlsI GCNGC 4 cut(s) 258, 475, 1027, 1066
Bme1390I CCNGG 8 cut(s) 107, 162, 277, 278, 381, 507, 517, 1191
Bme18I GGWCC 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 276
BmgBI CACGTC 1 cut(s) 372
BmgT120I GGNCC 3 cut(s) 157, 548, 1252
BmiI GGNNCC 4 cut(s) 159, 217, 550, 1253
BmrFI CCNGG 8 cut(s) 107, 162, 277, 278, 381, 507, 517, 1191
BmrI ACTGGG 3 cut(s) 71, 713, 929
BmsI GCATC 8 cut(s) 100, 271, 562, 580, 644, 778, 1123, 1141
BmtI GCTAGC 1 cut(s) 339
BmuI ACTGGG 3 cut(s) 71, 713, 929
BplI GAGNNNNNCTC 2 cut(s) 730, 762
BpmI CTGGAG 1 cut(s) 333
Bpu10I CCTNAGC 2 cut(s) 153, 785
BpuEI CTTGAG 2 cut(s) 185, 674
BpuMI CCSGG 4 cut(s) 107, 277, 278, 517
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 8 cut(s) 160, 275, 276, 379, 403, 506, 673, 1189
BsaXI ACNNNNNCTCC 2 cut(s) 786, 816
Bsc4I CCNNNNNNNGG 6 cut(s) 303, 398, 507, 558, 656, 1041
Bse1I ACTGG 4 cut(s) 77, 521, 708, 935
Bse21I CCTNAGG 1 cut(s) 65
BseBI CCWGG 4 cut(s) 162, 381, 507, 1191
BseDI CCNNGG 8 cut(s) 160, 275, 276, 379, 403, 506, 673, 1189
BseGI GGATG 2 cut(s) 115, 286
BseLI CCNNNNNNNGG 6 cut(s) 303, 398, 507, 558, 656, 1041
BseMII CTCAG 2 cut(s) 79, 876
BseNI ACTGG 4 cut(s) 77, 521, 708, 935
BseRI GAGGAG 1 cut(s) 319
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 4 cut(s) 243, 485, 1037, 1076
Bsh1236I CGCG 1 cut(s) 654
BshFI GGCC 5 cut(s) 158, 402, 520, 1008, 1254
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 4 cut(s) 107, 174, 277, 517
BslFI GGGAC 2 cut(s) 916, 1187
BslI CCNNNNNNNGG 6 cut(s) 303, 398, 507, 558, 656, 1041
BsmAI GTCTC 2 cut(s) 369, 694
BsmFI GGGAC 2 cut(s) 916, 1187
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 5 cut(s) 158, 402, 520, 1008, 1254
Bso31I GGTCTC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 276
Bsp1286I GDGCHC 3 cut(s) 276, 355, 690
Bsp143I GATC 8 cut(s) 443, 455, 512, 616, 743, 941, 993, 1262
BspACI CCGC 1 cut(s) 654
BspANI GGCC 5 cut(s) 158, 402, 520, 1008, 1254
BspCNI CTCAG 2 cut(s) 78, 877
BspFNI CGCG 1 cut(s) 654
BspLI GGNNCC 4 cut(s) 159, 217, 550, 1253
BspMAI CTGCAG 1 cut(s) 560
BspOI GCTAGC 1 cut(s) 339
BspPI GGATC 6 cut(s) 451, 463, 507, 624, 751, 1270
BspTNI GGTCTC 1 cut(s) 369
BsrI ACTGG 4 cut(s) 77, 521, 708, 935
BssECI CCNNGG 8 cut(s) 160, 275, 276, 379, 403, 506, 673, 1189
BssMI GATC 8 cut(s) 443, 455, 512, 616, 743, 941, 993, 1262
BssT1I CCWWGG 2 cut(s) 403, 673
Bst2UI CCWGG 4 cut(s) 162, 381, 507, 1191
Bst4CI ACNGT 3 cut(s) 565, 880, 1295
BstAPI GCANNNNNTGC 1 cut(s) 1160
BstC8I GCNNGC 5 cut(s) 179, 244, 286, 337, 1305
BstDEI CTNAG 5 cut(s) 65, 153, 785, 885, 1311
BstEII GGTNACC 1 cut(s) 292
BstF5I GGATG 2 cut(s) 115, 286
BstFNI CGCG 1 cut(s) 654
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 256, 311
BstKTI GATC 8 cut(s) 446, 458, 515, 619, 746, 944, 996, 1265
BstMAI GTCTC 2 cut(s) 369, 694
BstMBI GATC 8 cut(s) 443, 455, 512, 616, 743, 941, 993, 1262
BstMWI GCNNNNNNNGC 7 cut(s) 131, 183, 517, 881, 905, 1031, 1160
BstNI CCWGG 4 cut(s) 162, 381, 507, 1191
BstPI GGTNACC 1 cut(s) 292
BstSCI CCNGG 8 cut(s) 105, 160, 275, 276, 379, 505, 515, 1189
BstSFI CTRYAG 1 cut(s) 556
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 4 cut(s) 243, 485, 1037, 1076
BstX2I RGATCY 2 cut(s) 743, 1262
BstYI RGATCY 2 cut(s) 743, 1262
Bsu36I CCTNAGG 1 cut(s) 65
BsuRI GGCC 5 cut(s) 158, 402, 520, 1008, 1254
BtrI CACGTC 1 cut(s) 372
BtsCI GGATG 2 cut(s) 115, 286
BtsI GCAGTG 1 cut(s) 457
BtsIMutI CAGTG 3 cut(s) 349, 457, 885
Cac8I GCNNGC 5 cut(s) 179, 244, 286, 337, 1305
CaiI CAGNNNCTG 2 cut(s) 575, 884
CfoI GCGC 2 cut(s) 256, 311
Cfr13I GGNCC 3 cut(s) 157, 548, 1252
Cfr9I CCCGGG 1 cut(s) 276
Csp6I GTAC 2 cut(s) 422, 1169
CviAII CATG 2 cut(s) 182, 438
CviQI GTAC 2 cut(s) 422, 1169
DdeI CTNAG 5 cut(s) 65, 153, 785, 885, 1311
DpnI GATC 8 cut(s) 445, 457, 514, 618, 745, 943, 995, 1264
DpnII GATC 8 cut(s) 443, 455, 512, 616, 743, 941, 993, 1262
DraIII CACNNNGTG 1 cut(s) 656
EaeI YGGCCR 3 cut(s) 400, 518, 1006
Eco130I CCWWGG 2 cut(s) 403, 673
Eco24I GRGCYC 2 cut(s) 355, 690
Eco31I GGTCTC 1 cut(s) 369
Eco32I GATATC 1 cut(s) 955
Eco47I GGWCC 1 cut(s) 548
Eco47III AGCGCT 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 276
Eco91I GGTNACC 1 cut(s) 292
EcoO109I RGGNCCY 2 cut(s) 157, 1252
EcoO65I GGTNACC 1 cut(s) 292
EcoRII CCWGG 4 cut(s) 160, 379, 505, 1189
EcoRV GATATC 1 cut(s) 955
EcoT14I CCWWGG 2 cut(s) 403, 673
EcoT22I ATGCAT 2 cut(s) 910, 1138
EcoT38I GRGCYC 2 cut(s) 355, 690
ErhI CCWWGG 2 cut(s) 403, 673
FaeI CATG 2 cut(s) 185, 441
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 2 cut(s) 916, 1187
FatI CATG 2 cut(s) 181, 437
FbaI TGATCA 1 cut(s) 993
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 4 cut(s) 257, 474, 1026, 1065
FokI GGATG 2 cut(s) 122, 293
FriOI GRGCYC 2 cut(s) 355, 690
Fsp4HI GCNGC 4 cut(s) 257, 474, 1026, 1065
FspBI CTAG 3 cut(s) 336, 896, 1130
GlaI GCGC 2 cut(s) 255, 310
GluI GCNGC 4 cut(s) 257, 474, 1026, 1065
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 5 cut(s) 158, 402, 520, 1008, 1254
HapII CCGG 4 cut(s) 107, 174, 277, 517
HhaI GCGC 2 cut(s) 256, 311
Hin1II CATG 2 cut(s) 185, 441
Hin6I GCGC 2 cut(s) 254, 309
HinP1I GCGC 2 cut(s) 254, 309
HindIII AAGCTT 1 cut(s) 1274
HinfI GANTC 5 cut(s) 90, 315, 718, 1144, 1185
HpaII CCGG 4 cut(s) 107, 174, 277, 517
HphI GGTGA 5 cut(s) 112, 304, 366, 721, 1153
Hpy166II GTNNAC 3 cut(s) 103, 580, 811
Hpy188I TCNGA 1 cut(s) 1267
Hpy188III TCNNGA 6 cut(s) 30, 56, 587, 818, 844, 1148
Hpy8I GTNNAC 3 cut(s) 103, 580, 811
HpyAV CCTTC 6 cut(s) 62, 325, 733, 973, 1054, 1216
HpyCH4III ACNGT 3 cut(s) 565, 880, 1295
HpyCH4IV ACGT 3 cut(s) 357, 371, 582
HpyF10VI GCNNNNNNNGC 7 cut(s) 131, 183, 517, 881, 905, 1031, 1160
HpyF3I CTNAG 5 cut(s) 65, 153, 785, 885, 1311
HpySE526I ACGT 3 cut(s) 357, 371, 582
Hsp92II CATG 2 cut(s) 185, 441
HspAI GCGC 2 cut(s) 254, 309
Ksp22I TGATCA 1 cut(s) 993
Kzo9I GATC 8 cut(s) 443, 455, 512, 616, 743, 941, 993, 1262
LmnI GCTCC 2 cut(s) 306, 1168
Lsp1109I GCAGC 4 cut(s) 243, 485, 1037, 1076
LweI GCATC 8 cut(s) 100, 271, 562, 580, 644, 778, 1123, 1141
MaeI CTAG 3 cut(s) 336, 896, 1130
MaeII ACGT 3 cut(s) 357, 371, 582
MaeIII GTNAC 5 cut(s) 292, 372, 523, 565, 1141
MalI GATC 8 cut(s) 445, 457, 514, 618, 745, 943, 995, 1264
MboI GATC 8 cut(s) 443, 455, 512, 616, 743, 941, 993, 1262
MboII GAAGA 1 cut(s) 718
MflI RGATCY 2 cut(s) 743, 1262
MhlI GDGCHC 3 cut(s) 276, 355, 690
MlsI TGGCCA 2 cut(s) 402, 1008
MluCI AATT 5 cut(s) 478, 753, 847, 853, 1001
MluNI TGGCCA 2 cut(s) 402, 1008
MlyI GAGTC 3 cut(s) 99, 324, 1138
MnlI CCTC 8 cut(s) 74, 91, 297, 389, 708, 742, 788, 1335
Mox20I TGGCCA 2 cut(s) 402, 1008
Mph1103I ATGCAT 2 cut(s) 910, 1138
MroXI GAANNNNTTC 1 cut(s) 722
MscI TGGCCA 2 cut(s) 402, 1008
MseI TTAA 2 cut(s) 84, 852
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 2 cut(s) 402, 1008
MspI CCGG 4 cut(s) 107, 174, 277, 517
MspR9I CCNGG 8 cut(s) 107, 162, 277, 278, 381, 507, 517, 1191
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 4 cut(s) 162, 381, 507, 1191
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 7 cut(s) 131, 183, 517, 881, 905, 1031, 1160
NciI CCSGG 4 cut(s) 107, 277, 278, 517
NdeII GATC 8 cut(s) 443, 455, 512, 616, 743, 941, 993, 1262
NheI GCTAGC 1 cut(s) 335
NlaIII CATG 2 cut(s) 185, 441
NlaIV GGNNCC 4 cut(s) 159, 217, 550, 1253
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 4 cut(s) 292, 372, 565, 1141
NsiI ATGCAT 2 cut(s) 910, 1138
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 722
PfeI GAWTC 2 cut(s) 718, 1185
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 4 cut(s) 258, 475, 1027, 1066
PleI GAGTC 3 cut(s) 98, 323, 1138
PpsI GAGTC 3 cut(s) 98, 323, 1138
PsiI TTATAA 1 cut(s) 15
Psp6I CCWGG 4 cut(s) 160, 379, 505, 1189
PspEI GGTNACC 1 cut(s) 292
PspGI CCWGG 4 cut(s) 160, 379, 505, 1189
PspN4I GGNNCC 4 cut(s) 159, 217, 550, 1253
PspPI GGNCC 3 cut(s) 157, 548, 1252
PsrI GAACNNNNNNTAC 2 cut(s) 653, 685
PstI CTGCAG 1 cut(s) 560
PstNI CAGNNNCTG 2 cut(s) 575, 884
PsuI RGATCY 2 cut(s) 743, 1262
RsaI GTAC 2 cut(s) 423, 1170
RsaNI GTAC 2 cut(s) 422, 1169
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 2 cut(s) 84, 852
SatI GCNGC 4 cut(s) 257, 474, 1026, 1065
Sau3AI GATC 8 cut(s) 443, 455, 512, 616, 743, 941, 993, 1262
Sau96I GGNCC 3 cut(s) 157, 548, 1252
SchI GAGTC 3 cut(s) 99, 324, 1138
ScrFI CCNGG 8 cut(s) 107, 162, 277, 278, 381, 507, 517, 1191
SduI GDGCHC 3 cut(s) 276, 355, 690
SfaNI GCATC 8 cut(s) 100, 271, 562, 580, 644, 778, 1123, 1141
SfcI CTRYAG 1 cut(s) 556
SinI GGWCC 1 cut(s) 548
SmaI CCCGGG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 200, 689
SmoI CTYRAG 2 cut(s) 200, 689
Sse9I AATT 5 cut(s) 478, 753, 847, 853, 1001
SsiI CCGC 1 cut(s) 654
SspMI CTAG 3 cut(s) 336, 896, 1130
StyD4I CCNGG 8 cut(s) 105, 160, 275, 276, 379, 505, 515, 1189
StyI CCWWGG 2 cut(s) 403, 673
TaaI ACNGT 3 cut(s) 565, 880, 1295
TaiI ACGT 3 cut(s) 360, 374, 585
TaqI TCGA 3 cut(s) 638, 940, 1299
TasI AATT 5 cut(s) 478, 753, 847, 853, 1001
TatI WGTACW 2 cut(s) 421, 1168
TfiI GAWTC 2 cut(s) 718, 1185
Tru1I TTAA 2 cut(s) 84, 852
Tru9I TTAA 2 cut(s) 84, 852
TscAI CASTG 3 cut(s) 349, 457, 885
TseFI GTSAC 4 cut(s) 292, 372, 565, 1141
TseI GCWGC 4 cut(s) 256, 473, 1025, 1064
Tsp45I GTSAC 4 cut(s) 292, 372, 565, 1141
TspGWI ACGGA 1 cut(s) 102
TspMI CCCGGG 1 cut(s) 276
TspRI CASTG 3 cut(s) 349, 457, 885
Van91I CCANNNNNTGG 1 cut(s) 656
VpaK11BI GGWCC 1 cut(s) 548
XapI RAATTY 4 cut(s) 478, 753, 847, 1001
XmaI CCCGGG 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 810
XmnI GAANNNNTTC 1 cut(s) 722
XspI CTAG 3 cut(s) 336, 896, 1130
Zsp2I ATGCAT 2 cut(s) 910, 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.