Rw6G002120

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000427
Physical Location & Seq
Reverse (-)
3323819 .. 3326106
2288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002120.1

Sequence Viewer

Length: 1509 bp
ATGGCCAAAACTCCGTTGATCAGCTCAAATTCTTGGGTTGTTGGTCACTGGCAGTCTGGCACTCCATTACTGCCATTATCATCCATGGCTAACTATCTTCTCCTCTCGTTTTTGCTTCTAATTTGCATTCTATCATATGGCTCAGAAACACTCACAGCCAAAAGGCCCAAAATCACAATTGAAGAAGCTACTGTTGATGATCTCCAGCTTGCTTTCAAGCTCAACACTCTGACCTCAAGGCAACTTGTTCTGTTCTACATGGACAAGATTCAAAAACTGAACAAAAACCTTAGGGCTGTGATAGAGGGGAACCCTGATGCTTTATCCCAAGCTGATATAGCTGACAAACAACACAGAGAATGCAACCACACACTGCCTAAGCTTCATGGCATTCCTATTCTGCTCAAGGATACCATAGCAACCAAGGACAAACTCAACACCACAGCTGGATCGTTTGCCTTGTTGGGATCCATTGCTCCTCGAGATGCAGGTGTTGTTTCTAAGCTAAGGAGGTCTGGAGCTATCATTTTAGGGAAGGCTAGCTTGAGTGAATGGTGCCATTTCAGGTCATTCCTAGCACCTTCTGGATGGAGCGCCAGAGGTTCCCAAGGGATGAATCCTTACAATTCATCGGTGGAAGTTTGTGGATCAAGTAGTGGACCTGCAATATCAGTAGCTGCTAATATGGTTTCTGTGACATTAGGGACGGAGACTGACAGCTCCATTATCTGTCCGGCTAGCTTTAACTCTGTGGTGGGCATCAAACCCACTGTTGGTCTCACTAGTCGAGCAGGAGTCATACCAATCAGTCCAAGACAGGACACAGTTGGGCCGATTTGTAGGACTGTAGCGGATGCTGTTCACGTCCTTGATATCATTGTTGGCATTGATAGGAATGATAATGCAACAAGTGAAGCATCAAAGTACATCCCAGTGGGTGGCTATGGACAGTTTCTCAAGCATAATGGCCTCAAAGGAAAGAGACTGGGGATAGTAAATATGTTATTTGATTCAATCAAGGACGATGCTTTTCTGAAACAAACTTTTGTGCAGCATTTTACCACACTGAGGAAACAAGGTGCAATTTTGGTTGACAATCTTGAAATAGCCAATATTGAAGATGTTCTGGATTCTCATTCAAGTGGAGAATTTGATGCTATATTAGCTGAATTCAAAATAGCCTTGAATGAGTACCTAAGTGACCTAGTAAAATCTCCAGTGAAGTCCTTAAGGGAGGTGATAGAGTTCAACAAGAATCATTCAAAGGTGGAAAATATCAATGAATATGGGCAAGATGTCTTCGAATTAGCTGAGAAGACTAATGGGATGGGACCAAAAGAGCAGGAGGCATTGTCAAATTTAGAAAGACTATCTAGACAGGGGTTCAAGAAGTTGATGACCGATCATAGCCTAGATGCCTTGGTGGCTTATGCTAACAGTGCTTCTAGTATCCTCGCAATTGGTGGTTTTCCGGGCATTGTTGTGCCGGCAGGATATCATAACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

502

Amino Acids

54.26

Weight (kDa)

7.19

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 82 - 475 3.3e-65 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
Acc36I ACCTGC 2 cut(s) 479, 670
AccB1I GGYRCC 1 cut(s) 555
AccB7I CCANNNNNTGG 1 cut(s) 938
AciI CCGC 1 cut(s) 851
AclWI GGATC 4 cut(s) 457, 462, 475, 655
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 28, 1148, 1169, 1357
AfaI GTAC 2 cut(s) 926, 1193
AfiI CCNNNNNNNGG 3 cut(s) 773, 938, 1068
AflII CTTAAG 1 cut(s) 1228
AhlI ACTAGT 1 cut(s) 782
AjiI CACGTC 1 cut(s) 865
Alw26I GTCTC 3 cut(s) 704, 782, 976
AlwI GGATC 4 cut(s) 457, 462, 475, 655
AlwNI CAGNNNCTG 1 cut(s) 677
Ama87I CYCGRG 1 cut(s) 480
AoxI GGCC 4 cut(s) 3, 164, 830, 967
ApeKI GCWGC 2 cut(s) 677, 1051
ApoI RAATTY 4 cut(s) 28, 1148, 1169, 1357
Asp700I GAANNNNTTC 1 cut(s) 1122
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 4 cut(s) 165, 659, 830, 1331
AsuC2I CCSGG 1 cut(s) 1473
AsuHPI GGTGA 1 cut(s) 1249
AsuII TTCGAA 1 cut(s) 1302
AsuNHI GCTAGC 2 cut(s) 539, 737
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 2 cut(s) 659, 1331
AxyI CCTNAGG 1 cut(s) 290
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 555
BbsI GAAGAC 2 cut(s) 1291, 1322
BbvI GCAGC 2 cut(s) 664, 1063
BccI CCATC 2 cut(s) 582, 1321
BciVI GTATCC 2 cut(s) 403, 1460
BclI TGATCA 1 cut(s) 18
BcnI CCSGG 1 cut(s) 1473
BcoDI GTCTC 3 cut(s) 704, 782, 976
BcuI ACTAGT 1 cut(s) 782
BfaI CTAG 8 cut(s) 540, 575, 738, 783, 1205, 1374, 1412, 1446
BfmI CTRYAG 1 cut(s) 846
BfoI RGCGCY 1 cut(s) 597
BfrI CTTAAG 1 cut(s) 1228
BfuAI ACCTGC 2 cut(s) 479, 670
BfuI GTATCC 2 cut(s) 403, 1460
BglI GCCNNNNNGGC 1 cut(s) 1424
BisI GCNGC 2 cut(s) 678, 1052
BlsI GCNGC 2 cut(s) 679, 1053
Bme1390I CCNGG 1 cut(s) 1473
Bme18I GGWCC 2 cut(s) 659, 1331
BmeT110I CYCGRG 1 cut(s) 480
BmgBI CACGTC 1 cut(s) 865
BmgT120I GGNCC 4 cut(s) 165, 659, 830, 1331
BmiI GGNNCC 5 cut(s) 311, 469, 557, 604, 1332
BmrFI CCNGG 1 cut(s) 1473
BmrI ACTGGG 2 cut(s) 926, 995
BmsI GCATC 8 cut(s) 307, 475, 768, 844, 926, 1015, 1144, 1405
BmtI GCTAGC 2 cut(s) 543, 741
BmuI ACTGGG 2 cut(s) 926, 995
BpiI GAAGAC 2 cut(s) 1291, 1322
BpmI CTGGAG 3 cut(s) 188, 537, 1200
Bpu10I CCTNAGC 2 cut(s) 378, 506
Bpu14I TTCGAA 1 cut(s) 1302
BpuEI CTTGAG 4 cut(s) 220, 389, 565, 941
BpuMI CCSGG 1 cut(s) 1473
BsaI GGTCTC 1 cut(s) 782
BsaJI CCNNGG 4 cut(s) 84, 423, 607, 1419
BsaXI ACNNNNNCTCC 2 cut(s) 1337, 1367
Bsc4I CCNNNNNNNGG 3 cut(s) 773, 938, 1068
Bse118I RCCGGY 1 cut(s) 1486
Bse1I ACTGG 4 cut(s) 53, 932, 990, 1217
Bse21I CCTNAGG 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 471
BseDI CCNNGG 4 cut(s) 84, 423, 607, 1419
BseGI GGATG 6 cut(s) 80, 593, 618, 859, 927, 1332
BseLI CCNNNNNNNGG 3 cut(s) 773, 938, 1068
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 3 cut(s) 156, 1058, 1302
BseNI ACTGG 4 cut(s) 53, 932, 990, 1217
BseRI GAGGAG 2 cut(s) 92, 468
BseXI GCAGC 2 cut(s) 664, 1063
BsgI GTGCAG 1 cut(s) 1070
BshFI GGCC 4 cut(s) 5, 166, 832, 969
BshNI GGYRCC 1 cut(s) 555
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 3 cut(s) 734, 1472, 1487
BslFI GGGAC 2 cut(s) 718, 1344
BslI CCNNNNNNNGG 3 cut(s) 773, 938, 1068
BsmAI GTCTC 3 cut(s) 704, 782, 976
BsmFI GGGAC 2 cut(s) 718, 1344
BsmI GAATGC 3 cut(s) 126, 365, 390
BsnI GGCC 4 cut(s) 5, 166, 832, 969
Bso31I GGTCTC 1 cut(s) 782
BsoBI CYCGRG 1 cut(s) 480
Bsp119I TTCGAA 1 cut(s) 1302
Bsp143I GATC 6 cut(s) 18, 199, 449, 467, 647, 1402
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 1 cut(s) 851
BspANI GGCC 4 cut(s) 5, 166, 832, 969
BspCNI CTCAG 3 cut(s) 155, 1059, 1303
BspLI GGNNCC 5 cut(s) 311, 469, 557, 604, 1332
BspMI ACCTGC 2 cut(s) 479, 670
BspOI GCTAGC 2 cut(s) 543, 741
BspPI GGATC 4 cut(s) 457, 462, 475, 655
BspT104I TTCGAA 1 cut(s) 1302
BspT107I GGYRCC 1 cut(s) 555
BspTI CTTAAG 1 cut(s) 1228
BspTNI GGTCTC 1 cut(s) 782
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 1 cut(s) 1486
BsrI ACTGG 4 cut(s) 53, 932, 990, 1217
BssAI RCCGGY 1 cut(s) 1486
BssECI CCNNGG 4 cut(s) 84, 423, 607, 1419
BssMI GATC 6 cut(s) 18, 199, 449, 467, 647, 1402
BssT1I CCWWGG 4 cut(s) 84, 423, 607, 1419
Bst4CI ACNGT 6 cut(s) 193, 772, 826, 847, 951, 1439
BstAFI CTTAAG 1 cut(s) 1228
BstBI TTCGAA 1 cut(s) 1302
BstC8I GCNNGC 4 cut(s) 210, 541, 739, 1488
BstDEI CTNAG 8 cut(s) 142, 290, 378, 501, 506, 1067, 1196, 1311
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 6 cut(s) 80, 593, 618, 859, 927, 1332
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 6 cut(s) 21, 202, 452, 470, 650, 1405
BstMAI GTCTC 3 cut(s) 704, 782, 976
BstMBI GATC 6 cut(s) 18, 199, 449, 467, 647, 1402
BstMWI GCNNNNNNNGC 4 cut(s) 338, 1163, 1424, 1439
BstSCI CCNGG 1 cut(s) 1471
BstSFI CTRYAG 1 cut(s) 846
BstV1I GCAGC 2 cut(s) 664, 1063
BstV2I GAAGAC 2 cut(s) 1291, 1322
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
Bsu36I CCTNAGG 1 cut(s) 290
BsuI GTATCC 2 cut(s) 403, 1460
BsuRI GGCC 4 cut(s) 5, 166, 832, 969
BtgI CCRYGG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 865
BtsCI GGATG 6 cut(s) 80, 593, 618, 859, 927, 1332
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 7 cut(s) 46, 371, 768, 939, 1064, 1224, 1444
BveI ACCTGC 2 cut(s) 479, 670
Cac8I GCNNGC 4 cut(s) 210, 541, 739, 1488
CaiI CAGNNNCTG 1 cut(s) 677
CfoI GCGC 1 cut(s) 596
Cfr10I RCCGGY 1 cut(s) 1486
Cfr13I GGNCC 4 cut(s) 165, 659, 830, 1331
Csp6I GTAC 2 cut(s) 925, 1192
CviAII CATG 3 cut(s) 85, 259, 386
CviQI GTAC 2 cut(s) 925, 1192
DdeI CTNAG 8 cut(s) 142, 290, 378, 501, 506, 1067, 1196, 1311
DpnI GATC 6 cut(s) 20, 201, 451, 469, 649, 1404
DpnII GATC 6 cut(s) 18, 199, 449, 467, 647, 1402
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 4 cut(s) 84, 423, 607, 1419
Eco31I GGTCTC 1 cut(s) 782
Eco32I GATATC 2 cut(s) 874, 1496
Eco47I GGWCC 2 cut(s) 659, 1331
Eco81I CCTNAGG 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 480
EcoRI GAATTC 1 cut(s) 1169
EcoRV GATATC 2 cut(s) 874, 1496
EcoT14I CCWWGG 4 cut(s) 84, 423, 607, 1419
ErhI CCWWGG 4 cut(s) 84, 423, 607, 1419
FaeI CATG 3 cut(s) 88, 262, 389
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 2 cut(s) 718, 1344
FatI CATG 3 cut(s) 84, 258, 385
FauNDI CATATG 1 cut(s) 136
FbaI TGATCA 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 678, 1052
FokI GGATG 6 cut(s) 67, 600, 625, 866, 914, 1339
Fsp4HI GCNGC 2 cut(s) 678, 1052
FspBI CTAG 8 cut(s) 540, 575, 738, 783, 1205, 1374, 1412, 1446
GlaI GCGC 1 cut(s) 595
GluI GCNGC 2 cut(s) 678, 1052
GsuI CTGGAG 3 cut(s) 188, 537, 1200
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 4 cut(s) 5, 166, 832, 969
HapII CCGG 3 cut(s) 734, 1472, 1487
HhaI GCGC 1 cut(s) 596
Hin1II CATG 3 cut(s) 88, 262, 389
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 6 cut(s) 268, 616, 795, 1010, 1130, 1255
HpaII CCGG 3 cut(s) 734, 1472, 1487
HphI GGTGA 1 cut(s) 1249
Hpy166II GTNNAC 3 cut(s) 659, 862, 1093
Hpy188I TCNGA 3 cut(s) 145, 231, 1035
Hpy188III TCNNGA 7 cut(s) 482, 516, 585, 1100, 1127, 1374, 1387
Hpy8I GTNNAC 3 cut(s) 659, 862, 1093
HpyAV CCTTC 2 cut(s) 529, 591
HpyCH4III ACNGT 6 cut(s) 193, 772, 826, 847, 951, 1439
HpyCH4IV ACGT 1 cut(s) 864
HpyCH4V TGCA 7 cut(s) 126, 363, 488, 665, 905, 1051, 1082
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 1163, 1424, 1439
HpyF3I CTNAG 8 cut(s) 142, 290, 378, 501, 506, 1067, 1196, 1311
HpySE526I ACGT 1 cut(s) 864
Hsp92II CATG 3 cut(s) 88, 262, 389
HspAI GCGC 1 cut(s) 594
KroI GCCGGC 1 cut(s) 1486
KroNI GCCGGC 1 cut(s) 1488
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 6 cut(s) 18, 199, 449, 467, 647, 1402
LmnI GCTCC 4 cut(s) 481, 518, 591, 725
Lsp1109I GCAGC 2 cut(s) 664, 1063
LweI GCATC 8 cut(s) 307, 475, 768, 844, 926, 1015, 1144, 1405
MaeI CTAG 8 cut(s) 540, 575, 738, 783, 1205, 1374, 1412, 1446
MaeII ACGT 1 cut(s) 864
MaeIII GTNAC 3 cut(s) 44, 694, 1199
MalI GATC 6 cut(s) 20, 201, 451, 469, 649, 1404
MboI GATC 6 cut(s) 18, 199, 449, 467, 647, 1402
MboII GAAGA 5 cut(s) 89, 194, 1130, 1291, 1327
MfeI CAATTG 2 cut(s) 177, 1458
MflI RGATCY 1 cut(s) 467
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 804
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 1486
MroXI GAANNNNTTC 1 cut(s) 1122
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 744, 1229, 1507
MslI CAYNNNNRTG 3 cut(s) 932, 1140, 1481
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 446
MspCI CTTAAG 1 cut(s) 1228
MspI CCGG 3 cut(s) 734, 1472, 1487
MspR9I CCNGG 1 cut(s) 1473
MunI CAATTG 2 cut(s) 177, 1458
Mva1269I GAATGC 3 cut(s) 126, 365, 390
MwoI GCNNNNNNNGC 4 cut(s) 338, 1163, 1424, 1439
NaeI GCCGGC 1 cut(s) 1488
NciI CCSGG 1 cut(s) 1473
NcoI CCATGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 136
NdeII GATC 6 cut(s) 18, 199, 449, 467, 647, 1402
NgoMIV GCCGGC 1 cut(s) 1486
NheI GCTAGC 2 cut(s) 539, 737
NlaIII CATG 3 cut(s) 88, 262, 389
NlaIV GGNNCC 5 cut(s) 311, 469, 557, 604, 1332
NmuCI GTSAC 3 cut(s) 44, 694, 1199
NspV TTCGAA 1 cut(s) 1302
PaeR7I CTCGAG 1 cut(s) 480
PaqCI CACCTGC 1 cut(s) 479
PctI GAATGC 3 cut(s) 126, 365, 390
PdiI GCCGGC 1 cut(s) 1488
PdmI GAANNNNTTC 1 cut(s) 1122
PfeI GAWTC 5 cut(s) 268, 616, 1010, 1130, 1255
PflMI CCANNNNNTGG 1 cut(s) 938
PkrI GCNGC 2 cut(s) 679, 1053
PleI GAGTC 1 cut(s) 803
PpsI GAGTC 1 cut(s) 803
PspN4I GGNNCC 5 cut(s) 311, 469, 557, 604, 1332
PspPI GGNCC 4 cut(s) 165, 659, 830, 1331
PstNI CAGNNNCTG 1 cut(s) 677
PsuI RGATCY 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 926, 1193
RsaNI GTAC 2 cut(s) 925, 1192
RseI CAYNNNNRTG 3 cut(s) 932, 1140, 1481
SaqAI TTAA 3 cut(s) 744, 1229, 1507
SatI GCNGC 2 cut(s) 678, 1052
Sau3AI GATC 6 cut(s) 18, 199, 449, 467, 647, 1402
Sau96I GGNCC 4 cut(s) 165, 659, 830, 1331
SchI GAGTC 1 cut(s) 804
ScrFI CCNGG 1 cut(s) 1473
SfaNI GCATC 8 cut(s) 307, 475, 768, 844, 926, 1015, 1144, 1405
SfcI CTRYAG 1 cut(s) 846
Sfr274I CTCGAG 1 cut(s) 480
SfuI TTCGAA 1 cut(s) 1302
SinI GGWCC 2 cut(s) 659, 1331
SlaI CTCGAG 1 cut(s) 480
SmiMI CAYNNNNRTG 3 cut(s) 932, 1140, 1481
SmlI CTYRAG 6 cut(s) 235, 404, 480, 544, 956, 1228
SmoI CTYRAG 6 cut(s) 235, 404, 480, 544, 956, 1228
SpeI ACTAGT 1 cut(s) 782
SsiI CCGC 1 cut(s) 851
SspI AATATT 1 cut(s) 1114
SspMI CTAG 8 cut(s) 540, 575, 738, 783, 1205, 1374, 1412, 1446
StyD4I CCNGG 1 cut(s) 1471
StyI CCWWGG 4 cut(s) 84, 423, 607, 1419
TaaI ACNGT 6 cut(s) 193, 772, 826, 847, 951, 1439
TaiI ACGT 1 cut(s) 867
TaqI TCGA 3 cut(s) 481, 787, 1302
TaqII GACCGA 1 cut(s) 1415
TatI WGTACW 1 cut(s) 924
TfiI GAWTC 5 cut(s) 268, 616, 1010, 1130, 1255
Tru1I TTAA 3 cut(s) 744, 1229, 1507
Tru9I TTAA 3 cut(s) 744, 1229, 1507
TscAI CASTG 7 cut(s) 53, 378, 775, 939, 1071, 1224, 1444
TseFI GTSAC 3 cut(s) 44, 694, 1199
TseI GCWGC 2 cut(s) 677, 1051
Tsp45I GTSAC 3 cut(s) 44, 694, 1199
TspDTI ATGAA 4 cut(s) 374, 618, 629, 1296
TspGWI ACGGA 1 cut(s) 722
TspRI CASTG 7 cut(s) 53, 378, 775, 939, 1071, 1224, 1444
Van91I CCANNNNNTGG 1 cut(s) 938
Vha464I CTTAAG 1 cut(s) 1228
VpaK11BI GGWCC 2 cut(s) 659, 1331
XapI RAATTY 4 cut(s) 28, 1148, 1169, 1357
XbaI TCTAGA 1 cut(s) 1373
XhoI CTCGAG 1 cut(s) 480
XmnI GAANNNNTTC 1 cut(s) 1122
XspI CTAG 8 cut(s) 540, 575, 738, 783, 1205, 1374, 1412, 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.