RchiOBHm_Chr6g0244721

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000427
Physical Location & Seq
Reverse (-)
728902 .. 731622
2721 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21932

Sequence Viewer

Length: 1581 bp
ATGATAATTTCCTCATCGACTCCCATGACGAAACCAAGCTTCCCTCACATCTTATCTTTATTGCTTCTCATTCTTCTCTTCACATTATCATCTCAGCCAGGCACATTCGCAGCCCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTTATGCACAACCAACTCACCTCGAGGCAACTTGTCCAATATTACCTTTCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAACCCGGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCTACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCACTGCTTGGATCTGTCGTGCCTCGTGATGCCGGCGTGGTGGAGAAGTTGAGGAAAGCTGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCATATTTTAGGTCTAATAATACAGCACCAAATGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTAGCCGCAAATCTGGCAGCGGTGTCATTAGGTACTGAGACAGATGGTTCAATCTTATGTCCATCAGGTTACAACTCGGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGAGTTGTCCCAATTTCTCCAAGGCAGGATACTGTTGGACCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATAGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTTAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTGGAAATTACTAATTTGACTGAAATCTATTCTTCAAGCGATGAAGGTACTGCATTGTCTGTTGAGTTCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCCGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACGTATTTTTAGCTGCTGAAGCAACAAATGGGTTCGGAAAAACAGAGAAGCCGGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGCTGTGGTGACTCCTCACGCAGGTGTTTCAAGAGTACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCCGGATACGAGAACAATGGGAGACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCTACTAAGATTAGGAAGCCTCCTTCATTCAAAGGCTTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

526

Amino Acids

56.5

Weight (kDa)

9.48

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 65 - 460 1.5e-69 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1373
AbsI CCTCGAGG 1 cut(s) 184
Acc36I ACCTGC 1 cut(s) 1373
AccB1I GGYRCC 1 cut(s) 313
AccI GTMKAC 1 cut(s) 1044
AccII CGCG 1 cut(s) 918
AciI CCGC 7 cut(s) 115, 399, 561, 600, 612, 633, 647
AclWI GGATC 2 cut(s) 430, 1164
AcuI CTGAAG 1 cut(s) 1278
AfaI GTAC 4 cut(s) 661, 881, 1099, 1398
AfiI CCNNNNNNNGG 4 cut(s) 728, 893, 1213, 1382
AhlI ACTAGT 1 cut(s) 737
AjnI CCWGG 2 cut(s) 97, 1417
AleI CACNNNNGTG 1 cut(s) 1383
AluBI AGCT 9 cut(s) 39, 157, 470, 498, 1253, 1357, 1463, 1504, 1535
AluI AGCT 9 cut(s) 39, 157, 470, 498, 1253, 1357, 1463, 1504, 1535
Alw26I GTCTC 3 cut(s) 659, 863, 1450
AlwI GGATC 2 cut(s) 430, 1164
AlwNI CAGNNNCTG 1 cut(s) 812
Ama87I CYCGRG 1 cut(s) 184
ApeKI GCWGC 5 cut(s) 110, 609, 644, 812, 1253
AspLEI GCGC 4 cut(s) 271, 303, 554, 918
AspS9I GGNCC 2 cut(s) 306, 785
AsuC2I CCSGG 2 cut(s) 233, 263
AsuHPI GGTGA 4 cut(s) 172, 266, 1354, 1381
AsuNHI GCTAGC 1 cut(s) 494
AvaI CYCGRG 1 cut(s) 184
AvaII GGWCC 2 cut(s) 306, 785
BanI GGYRCC 1 cut(s) 313
BanII GRGCYC 1 cut(s) 1417
BauI CACGAG 1 cut(s) 435
BbvI GCAGC 5 cut(s) 122, 621, 656, 799, 1240
BccI CCATC 2 cut(s) 665, 697
BciT130I CCWGG 2 cut(s) 99, 1419
BciVI GTATCC 2 cut(s) 769, 1433
BcnI CCSGG 2 cut(s) 233, 263
BcoDI GTCTC 3 cut(s) 659, 863, 1450
BcuI ACTAGT 1 cut(s) 737
BfaI CTAG 4 cut(s) 251, 495, 738, 1358
BfoI RGCGCY 1 cut(s) 555
BfuAI ACCTGC 1 cut(s) 1373
BfuI GTATCC 2 cut(s) 769, 1433
BglI GCCNNNNNGGC 1 cut(s) 287
BisI GCNGC 7 cut(s) 111, 601, 610, 633, 645, 813, 1254
BlsI GCNGC 7 cut(s) 112, 602, 611, 634, 646, 814, 1255
BmcAI AGTACT 1 cut(s) 1398
Bme1390I CCNGG 4 cut(s) 99, 233, 263, 1419
Bme18I GGWCC 2 cut(s) 306, 785
BmeT110I CYCGRG 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 306, 785
BmiI GGNNCC 2 cut(s) 315, 1414
BmrFI CCNGG 4 cut(s) 99, 233, 263, 1419
BmrI ACTGGG 1 cut(s) 1157
BmsI GCATC 7 cut(s) 256, 430, 723, 799, 817, 849, 1351
BmtI GCTAGC 1 cut(s) 498
BmuI ACTGGG 1 cut(s) 1157
BpmI CTGGAG 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 520, 589
BpuMI CCSGG 2 cut(s) 233, 263
BsaI GGTCTC 1 cut(s) 1450
BsaJI CCNNGG 6 cut(s) 309, 378, 767, 984, 1173, 1417
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bsc4I CCNNNNNNNGG 4 cut(s) 728, 893, 1213, 1382
Bse118I RCCGGY 3 cut(s) 401, 443, 1291
Bse1I ACTGG 3 cut(s) 317, 1163, 1218
Bse3DI GCAATG 3 cut(s) 369, 855, 979
BseBI CCWGG 2 cut(s) 99, 1419
BseDI CCNNGG 6 cut(s) 309, 378, 767, 984, 1173, 1417
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 4 cut(s) 728, 893, 1213, 1382
BseMI GCAATG 3 cut(s) 369, 855, 979
BseMII CTCAG 5 cut(s) 107, 603, 654, 925, 1010
BseNI ACTGG 3 cut(s) 317, 1163, 1218
BseRI GAGGAG 1 cut(s) 1365
BseXI GCAGC 5 cut(s) 122, 621, 656, 799, 1240
BseYI CCCAGC 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 918
BshNI GGYRCC 1 cut(s) 313
BsiHKCI CYCGRG 1 cut(s) 184
BsiSI CCGG 6 cut(s) 233, 263, 402, 444, 1292, 1437
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 4 cut(s) 728, 893, 1213, 1382
BsmAI GTCTC 3 cut(s) 659, 863, 1450
BsmFI GGGAC 1 cut(s) 740
BsmI GAATGC 1 cut(s) 345
Bso31I GGTCTC 1 cut(s) 1450
BsoBI CYCGRG 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 1417
Bsp143I GATC 4 cut(s) 148, 422, 1169, 1221
BspACI CCGC 7 cut(s) 115, 399, 561, 600, 612, 633, 647
BspCNI CTCAG 5 cut(s) 106, 602, 655, 924, 1011
BspFNI CGCG 1 cut(s) 918
BspLI GGNNCC 2 cut(s) 315, 1414
BspMI ACCTGC 1 cut(s) 1373
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 2 cut(s) 430, 1164
BspT107I GGYRCC 1 cut(s) 313
BspTNI GGTCTC 1 cut(s) 1450
BsrDI GCAATG 3 cut(s) 369, 855, 979
BsrFI RCCGGY 3 cut(s) 401, 443, 1291
BsrI ACTGG 3 cut(s) 317, 1163, 1218
BssAI RCCGGY 3 cut(s) 401, 443, 1291
BssECI CCNNGG 6 cut(s) 309, 378, 767, 984, 1173, 1417
BssMI GATC 4 cut(s) 148, 422, 1169, 1221
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 5 cut(s) 309, 378, 767, 984, 1173
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 99, 1419
Bst4CI ACNGT 3 cut(s) 781, 802, 906
Bst6I CTCTTC 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 812
BstC8I GCNNGC 8 cut(s) 115, 159, 403, 445, 496, 838, 1293, 1533
BstDEI CTNAG 8 cut(s) 93, 333, 589, 663, 911, 926, 1019, 1539
BstENI CCTNNNNNAGG 1 cut(s) 1380
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 1 cut(s) 918
BstH2I RGCGCY 1 cut(s) 555
BstHHI GCGC 4 cut(s) 271, 303, 554, 918
BstKTI GATC 4 cut(s) 151, 425, 1172, 1224
BstMAI GTCTC 3 cut(s) 659, 863, 1450
BstMBI GATC 4 cut(s) 148, 422, 1169, 1221
BstMWI GCNNNNNNNGC 8 cut(s) 287, 558, 567, 606, 609, 641, 812, 1259
BstNI CCWGG 2 cut(s) 99, 1419
BstSCI CCNGG 4 cut(s) 97, 231, 261, 1417
BstUI CGCG 1 cut(s) 918
BstV1I GCAGC 5 cut(s) 122, 621, 656, 799, 1240
BstX2I RGATCY 1 cut(s) 422
BstXI CCANNNNNNTGG 1 cut(s) 546
BstYI RGATCY 1 cut(s) 422
BsuI GTATCC 2 cut(s) 769, 1433
BtgZI GCGATG 1 cut(s) 1104
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 1 cut(s) 413
BtsIMutI CAGTG 3 cut(s) 324, 413, 1170
BveI ACCTGC 1 cut(s) 1373
Cac8I GCNNGC 8 cut(s) 115, 159, 403, 445, 496, 838, 1293, 1533
CaiI CAGNNNCTG 1 cut(s) 812
CfoI GCGC 4 cut(s) 271, 303, 554, 918
Cfr10I RCCGGY 3 cut(s) 401, 443, 1291
Cfr13I GGNCC 2 cut(s) 306, 785
Csp6I GTAC 4 cut(s) 660, 880, 1098, 1397
CviAII CATG 2 cut(s) 25, 341
CviQI GTAC 4 cut(s) 660, 880, 1098, 1397
DdeI CTNAG 8 cut(s) 93, 333, 589, 663, 911, 926, 1019, 1539
DpnI GATC 4 cut(s) 150, 424, 1171, 1223
DpnII GATC 4 cut(s) 148, 422, 1169, 1221
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco130I CCWWGG 5 cut(s) 309, 378, 767, 984, 1173
Eco24I GRGCYC 1 cut(s) 1417
Eco31I GGTCTC 1 cut(s) 1450
Eco47I GGWCC 2 cut(s) 306, 785
Eco57I CTGAAG 1 cut(s) 1278
Eco88I CYCGRG 1 cut(s) 184
EcoNI CCTNNNNNAGG 1 cut(s) 1380
EcoRII CCWGG 2 cut(s) 97, 1417
EcoT14I CCWWGG 5 cut(s) 309, 378, 767, 984, 1173
EcoT22I ATGCAT 1 cut(s) 1138
EcoT38I GRGCYC 1 cut(s) 1417
ErhI CCWWGG 5 cut(s) 309, 378, 767, 984, 1173
FaeI CATG 2 cut(s) 28, 344
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 1 cut(s) 740
FatI CATG 2 cut(s) 24, 340
FauI CCCGC 1 cut(s) 122
FauNDI CATATG 1 cut(s) 1519
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 7 cut(s) 111, 601, 610, 633, 645, 813, 1254
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 1417
Fsp4HI GCNGC 7 cut(s) 111, 601, 610, 633, 645, 813, 1254
FspBI CTAG 4 cut(s) 251, 495, 738, 1358
GlaI GCGC 4 cut(s) 270, 302, 553, 917
GluI GCNGC 7 cut(s) 111, 601, 610, 633, 645, 813, 1254
GsaI CCCAGC 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 137
HaeII RGCGCY 1 cut(s) 555
HapII CCGG 6 cut(s) 233, 263, 402, 444, 1292, 1437
HhaI GCGC 4 cut(s) 271, 303, 554, 918
Hin1II CATG 2 cut(s) 28, 344
Hin6I GCGC 4 cut(s) 269, 301, 552, 916
HinP1I GCGC 4 cut(s) 269, 301, 552, 916
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 5 cut(s) 19, 120, 246, 1303, 1372
HpaII CCGG 6 cut(s) 233, 263, 402, 444, 1292, 1437
HphI GGTGA 4 cut(s) 172, 266, 1354, 1381
Hpy166II GTNNAC 4 cut(s) 259, 1045, 1317, 1481
Hpy188I TCNGA 5 cut(s) 221, 592, 1020, 1277, 1495
Hpy188III TCNNGA 4 cut(s) 437, 824, 1392, 1510
Hpy8I GTNNAC 4 cut(s) 259, 1045, 1317, 1481
Hpy99I CGWCG 1 cut(s) 923
HpyAV CCTTC 5 cut(s) 967, 1088, 1138, 1201, 1566
HpyCH4III ACNGT 3 cut(s) 781, 802, 906
HpyCH4IV ACGT 1 cut(s) 1242
HpyF10VI GCNNNNNNNGC 8 cut(s) 287, 558, 567, 606, 609, 641, 812, 1259
HpyF3I CTNAG 8 cut(s) 93, 333, 589, 663, 911, 926, 1019, 1539
HpySE526I ACGT 1 cut(s) 1242
Hsp92II CATG 2 cut(s) 28, 344
HspAI GCGC 4 cut(s) 269, 301, 552, 916
KroI GCCGGC 3 cut(s) 401, 443, 1291
KroNI GCCGGC 3 cut(s) 403, 445, 1293
Kzo9I GATC 4 cut(s) 148, 422, 1169, 1221
LmnI GCTCC 2 cut(s) 549, 1412
Lsp1109I GCAGC 5 cut(s) 122, 621, 656, 799, 1240
LweI GCATC 7 cut(s) 256, 430, 723, 799, 817, 849, 1351
MaeI CTAG 4 cut(s) 251, 495, 738, 1358
MaeII ACGT 1 cut(s) 1242
MaeIII GTNAC 4 cut(s) 321, 695, 1342, 1369
MalI GATC 4 cut(s) 150, 424, 1171, 1223
MboI GATC 4 cut(s) 148, 422, 1169, 1221
MboII GAAGA 4 cut(s) 65, 70, 1074, 1363
MfeI CAATTG 1 cut(s) 1404
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 1417
MluCI AATT 8 cut(s) 6, 759, 789, 1048, 1056, 1063, 1337, 1404
MlyI GAGTC 4 cut(s) 13, 114, 255, 1366
MmeI TCCRAC 2 cut(s) 527, 763
Mph1103I ATGCAT 1 cut(s) 1138
MroNI GCCGGC 3 cut(s) 401, 443, 1291
MseI TTAA 2 cut(s) 1011, 1130
MslI CAYNNNNRTG 1 cut(s) 1383
MspA1I CMGCKG 3 cut(s) 612, 647, 1463
MspI CCGG 6 cut(s) 233, 263, 402, 444, 1292, 1437
MspR9I CCNGG 4 cut(s) 99, 233, 263, 1419
MunI CAATTG 1 cut(s) 1404
Mva1269I GAATGC 1 cut(s) 345
MvaI CCWGG 2 cut(s) 99, 1419
MvnI CGCG 1 cut(s) 918
MwoI GCNNNNNNNGC 8 cut(s) 287, 558, 567, 606, 609, 641, 812, 1259
NaeI GCCGGC 3 cut(s) 403, 445, 1293
NciI CCSGG 2 cut(s) 233, 263
NdeI CATATG 1 cut(s) 1519
NdeII GATC 4 cut(s) 148, 422, 1169, 1221
NgoMIV GCCGGC 3 cut(s) 401, 443, 1291
NheI GCTAGC 1 cut(s) 494
NlaIII CATG 2 cut(s) 28, 344
NlaIV GGNNCC 2 cut(s) 315, 1414
NmuCI GTSAC 3 cut(s) 321, 1342, 1369
NsiI ATGCAT 1 cut(s) 1138
OliI CACNNNNGTG 1 cut(s) 1383
PaeR7I CTCGAG 1 cut(s) 184
PaqCI CACCTGC 1 cut(s) 1373
PctI GAATGC 1 cut(s) 345
PdiI GCCGGC 3 cut(s) 403, 445, 1293
PfeI GAWTC 1 cut(s) 1303
PkrI GCNGC 7 cut(s) 112, 602, 611, 634, 646, 814, 1255
PleI GAGTC 4 cut(s) 13, 114, 254, 1366
PpsI GAGTC 4 cut(s) 13, 114, 254, 1366
Psp6I CCWGG 2 cut(s) 97, 1417
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 2 cut(s) 97, 1417
PspN4I GGNNCC 2 cut(s) 315, 1414
PspPI GGNCC 2 cut(s) 306, 785
PspXI VCTCGAGB 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 1463
RsaI GTAC 4 cut(s) 661, 881, 1099, 1398
RsaNI GTAC 4 cut(s) 660, 880, 1098, 1397
RseI CAYNNNNRTG 1 cut(s) 1383
SaqAI TTAA 2 cut(s) 1011, 1130
SatI GCNGC 7 cut(s) 111, 601, 610, 633, 645, 813, 1254
Sau3AI GATC 4 cut(s) 148, 422, 1169, 1221
Sau96I GGNCC 2 cut(s) 306, 785
ScaI AGTACT 1 cut(s) 1398
SchI GAGTC 4 cut(s) 13, 114, 255, 1366
ScrFI CCNGG 4 cut(s) 99, 233, 263, 1419
SduI GDGCHC 1 cut(s) 1417
SfaNI GCATC 7 cut(s) 256, 430, 723, 799, 817, 849, 1351
Sfr274I CTCGAG 1 cut(s) 184
SinI GGWCC 2 cut(s) 306, 785
SlaI CTCGAG 1 cut(s) 184
SmiMI CAYNNNNRTG 1 cut(s) 1383
SmlI CTYRAG 3 cut(s) 184, 499, 604
SmoI CTYRAG 3 cut(s) 184, 499, 604
SpeI ACTAGT 1 cut(s) 737
Sse9I AATT 8 cut(s) 6, 759, 789, 1048, 1056, 1063, 1337, 1404
SsiI CCGC 7 cut(s) 115, 399, 561, 600, 612, 633, 647
SspI AATATT 1 cut(s) 203
SspMI CTAG 4 cut(s) 251, 495, 738, 1358
StyD4I CCNGG 4 cut(s) 97, 231, 261, 1417
StyI CCWWGG 5 cut(s) 309, 378, 767, 984, 1173
TaaI ACNGT 3 cut(s) 781, 802, 906
TaiI ACGT 1 cut(s) 1245
TaqI TCGA 5 cut(s) 17, 185, 742, 843, 1509
TaqII GACCGA 1 cut(s) 719
TasI AATT 8 cut(s) 6, 759, 789, 1048, 1056, 1063, 1337, 1404
TatI WGTACW 2 cut(s) 879, 1396
TauI GCSGC 2 cut(s) 603, 635
TfiI GAWTC 1 cut(s) 1303
Tru1I TTAA 2 cut(s) 1011, 1130
Tru9I TTAA 2 cut(s) 1011, 1130
TscAI CASTG 3 cut(s) 324, 420, 1170
TseFI GTSAC 3 cut(s) 321, 1342, 1369
TseI GCWGC 5 cut(s) 110, 609, 644, 812, 1253
Tsp45I GTSAC 3 cut(s) 321, 1342, 1369
TspDTI ATGAA 3 cut(s) 404, 1107, 1548
TspGWI ACGGA 1 cut(s) 936
TspRI CASTG 3 cut(s) 324, 420, 1170
VpaK11BI GGWCC 2 cut(s) 306, 785
XagI CCTNNNNNAGG 1 cut(s) 1380
XhoI CTCGAG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 1044
XspI CTAG 4 cut(s) 251, 495, 738, 1358
ZrmI AGTACT 1 cut(s) 1398
Zsp2I ATGCAT 1 cut(s) 1138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.