Rh6BG021200

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000427
Physical Location & Seq
Reverse (-)
3154880 .. 3179632
24753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG021200.1

Sequence Viewer

Length: 1563 bp
ATGATAATTTCCTCATCCACTGCCGTGGCGAAATTCCCTCACATCTCATGCTTATTGCTTCTCTTCACATTGTCATCTCTTCCAGGCACATTCGCAGCCCGCAGACTCTCACTAAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTCAAGCACAACCAACTCACCTCGAGGCAACTTGTTCAATATTACCTAGCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAATCCAGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTTTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCGCTGTTTGGATCTGTCGTGCCTCGTGATACCGGCGTGGTGGAGAAGTTGCGGAAAGCAGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGTCTACTAAAACCCCCTCTGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTTTCATTAGGCACTGAGACAGATGGTTCAATCTTATGTCCATCAGGTTATAACTCAGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCTAAGACAGGATACTGTCGGGCCAATTTGTAGGACAGTAGCAGATGTTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGGCTTAGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATAACTTCGGCAATGACACTGCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAGAGGCGGTGCTGTTTTGGTGGACAATTTGGAAATTGCTAATTTGACCGAAATCATTTTTTCAAGCGATGAAGATACTGCATTGTCTGTTGAGTGCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCAGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACCTATTTTTAGCAGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCAGGCATTGTTGAATTTTGCAAGGTGGACAAGAAATGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGTTGTGGTGACTCCTAAAGCAGATTTTTCACAGATACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCCGGATACGAGAACAATGGGAGACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCCACTAAGATTAGGAAGCCTCCTTCATTTAAAGGCTTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

520

Amino Acids

56.06

Weight (kDa)

9.46

Isoelectric Point (pI)

34.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 60 - 453 6.3e-69 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 681
AbsI CCTCGAGG 1 cut(s) 169
AccB1I GGYRCC 1 cut(s) 298
AccB7I CCANNNNNTGG 1 cut(s) 1195
AccI GTMKAC 1 cut(s) 509
AccII CGCG 1 cut(s) 900
AciI CCGC 8 cut(s) 100, 384, 448, 543, 582, 594, 629, 1011
AclWI GGATC 2 cut(s) 415, 1146
AcsI RAATTY 2 cut(s) 32, 1285
AcuI CTGAAG 1 cut(s) 1260
AfaI GTAC 1 cut(s) 863
AfiI CCNNNNNNNGG 4 cut(s) 528, 710, 875, 1195
AgsI TTSAA 9 cut(s) 151, 185, 556, 660, 1068, 1126, 1177, 1285, 1555
AhlI ACTAGT 1 cut(s) 719
AjnI CCWGG 2 cut(s) 82, 1399
AleI CACNNNNGTG 1 cut(s) 23
AluBI AGCT 6 cut(s) 142, 483, 1238, 1339, 1445, 1486
AluI AGCT 6 cut(s) 142, 483, 1238, 1339, 1445, 1486
Alw26I GTCTC 3 cut(s) 641, 845, 1432
AlwI GGATC 2 cut(s) 415, 1146
AlwNI CAGNNNCTG 1 cut(s) 1195
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 1 cut(s) 767
ApeKI GCWGC 5 cut(s) 95, 591, 614, 626, 1235
ApoI RAATTY 2 cut(s) 32, 1285
AspLEI GCGC 5 cut(s) 256, 288, 400, 536, 900
AspS9I GGNCC 2 cut(s) 291, 767
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 4 cut(s) 157, 251, 1336, 1363
AsuNHI GCTAGC 1 cut(s) 479
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 291
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BanI GGYRCC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 1399
BauI CACGAG 1 cut(s) 420
BbvI GCAGC 5 cut(s) 107, 603, 626, 638, 1247
BccI CCATC 2 cut(s) 647, 679
BceAI ACGGC 1 cut(s) 8
BciT130I CCWGG 2 cut(s) 84, 1401
BciVI GTATCC 2 cut(s) 751, 1415
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 3 cut(s) 641, 845, 1432
BcuI ACTAGT 1 cut(s) 719
BfaI CTAG 5 cut(s) 194, 236, 480, 720, 1340
BfoI RGCGCY 1 cut(s) 537
BfuI GTATCC 2 cut(s) 751, 1415
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 6 cut(s) 96, 583, 592, 615, 627, 1236
BlsI GCNGC 6 cut(s) 97, 584, 593, 616, 628, 1237
Bme1390I CCNGG 3 cut(s) 84, 218, 1401
Bme18I GGWCC 1 cut(s) 291
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 2 cut(s) 291, 767
BmiI GGNNCC 2 cut(s) 300, 1396
BmrFI CCNGG 3 cut(s) 84, 218, 1401
BmrI ACTGGG 1 cut(s) 1139
BmsI GCATC 4 cut(s) 241, 705, 799, 831
BmtI GCTAGC 1 cut(s) 483
BmuI ACTGGG 1 cut(s) 1139
BpmI CTGGAG 1 cut(s) 122
BpuEI CTTGAG 2 cut(s) 505, 571
BpuMI CCSGG 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 1432
BsaJI CCNNGG 6 cut(s) 24, 294, 363, 966, 1155, 1399
BsaXI ACNNNNNCTCC 2 cut(s) 997, 1027
Bsc4I CCNNNNNNNGG 4 cut(s) 528, 710, 875, 1195
Bse118I RCCGGY 2 cut(s) 386, 428
Bse1I ACTGG 3 cut(s) 302, 1145, 1200
Bse3DI GCAATG 3 cut(s) 354, 837, 961
BseBI CCWGG 2 cut(s) 84, 1401
BseDI CCNNGG 6 cut(s) 24, 294, 363, 966, 1155, 1399
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 4 cut(s) 528, 710, 875, 1195
BseMI GCAATG 3 cut(s) 354, 837, 961
BseMII CTCAG 5 cut(s) 585, 636, 699, 907, 992
BseNI ACTGG 3 cut(s) 302, 1145, 1200
BseRI GAGGAG 1 cut(s) 1018
BseXI GCAGC 5 cut(s) 107, 603, 626, 638, 1247
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 1 cut(s) 769
BshNI GGYRCC 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 4 cut(s) 218, 387, 429, 1419
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 4 cut(s) 528, 710, 875, 1195
BsmAI GTCTC 3 cut(s) 641, 845, 1432
BsmFI GGGAC 1 cut(s) 722
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 1 cut(s) 769
Bso31I GGTCTC 1 cut(s) 1432
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 1399
Bsp143I GATC 4 cut(s) 133, 407, 1151, 1203
BspACI CCGC 8 cut(s) 100, 384, 448, 543, 582, 594, 629, 1011
BspANI GGCC 1 cut(s) 769
BspCNI CTCAG 5 cut(s) 584, 637, 698, 906, 993
BspFNI CGCG 1 cut(s) 900
BspLI GGNNCC 2 cut(s) 300, 1396
BspOI GCTAGC 1 cut(s) 483
BspPI GGATC 2 cut(s) 415, 1146
BspT107I GGYRCC 1 cut(s) 298
BspTNI GGTCTC 1 cut(s) 1432
BsrDI GCAATG 3 cut(s) 354, 837, 961
BsrFI RCCGGY 2 cut(s) 386, 428
BsrI ACTGG 3 cut(s) 302, 1145, 1200
BssAI RCCGGY 2 cut(s) 386, 428
BssECI CCNNGG 6 cut(s) 24, 294, 363, 966, 1155, 1399
BssMI GATC 4 cut(s) 133, 407, 1151, 1203
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 4 cut(s) 294, 363, 966, 1155
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 2 cut(s) 84, 1401
Bst4CI ACNGT 3 cut(s) 763, 784, 888
Bst6I CTCTTC 2 cut(s) 68, 84
BstAPI GCANNNNNTGC 1 cut(s) 794
BstC8I GCNNGC 7 cut(s) 100, 144, 388, 481, 820, 1275, 1515
BstDEI CTNAG 9 cut(s) 318, 571, 645, 685, 749, 893, 908, 1001, 1521
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 1 cut(s) 900
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 5 cut(s) 256, 288, 400, 536, 900
BstKTI GATC 4 cut(s) 136, 410, 1154, 1206
BstMAI GTCTC 3 cut(s) 641, 845, 1432
BstMBI GATC 4 cut(s) 133, 407, 1151, 1203
BstMWI GCNNNNNNNGC 9 cut(s) 272, 540, 549, 588, 591, 623, 794, 904, 1241
BstNI CCWGG 2 cut(s) 84, 1401
BstSCI CCNGG 3 cut(s) 82, 216, 1399
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 5 cut(s) 107, 603, 626, 638, 1247
BstX2I RGATCY 1 cut(s) 407
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 1 cut(s) 407
BsuI GTATCC 2 cut(s) 751, 1415
BsuRI GGCC 1 cut(s) 769
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 1086
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 2 cut(s) 18, 960
BtsIMutI CAGTG 6 cut(s) 18, 309, 615, 642, 960, 1152
Cac8I GCNNGC 7 cut(s) 100, 144, 388, 481, 820, 1275, 1515
CaiI CAGNNNCTG 1 cut(s) 1195
CfoI GCGC 5 cut(s) 256, 288, 400, 536, 900
Cfr10I RCCGGY 2 cut(s) 386, 428
Cfr13I GGNCC 2 cut(s) 291, 767
Csp6I GTAC 1 cut(s) 862
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 2 cut(s) 48, 326
CviQI GTAC 1 cut(s) 862
DdeI CTNAG 9 cut(s) 318, 571, 645, 685, 749, 893, 908, 1001, 1521
DpnI GATC 4 cut(s) 135, 409, 1153, 1205
DpnII GATC 4 cut(s) 133, 407, 1151, 1203
DraI TTTAAA 1 cut(s) 1546
Eam1104I CTCTTC 2 cut(s) 68, 84
EarI CTCTTC 2 cut(s) 68, 84
Eco130I CCWWGG 4 cut(s) 294, 363, 966, 1155
Eco24I GRGCYC 1 cut(s) 1399
Eco31I GGTCTC 1 cut(s) 1432
Eco47I GGWCC 1 cut(s) 291
Eco57I CTGAAG 1 cut(s) 1260
Eco88I CYCGRG 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 82, 1399
EcoT14I CCWWGG 4 cut(s) 294, 363, 966, 1155
EcoT22I ATGCAT 1 cut(s) 1120
EcoT38I GRGCYC 1 cut(s) 1399
ErhI CCWWGG 4 cut(s) 294, 363, 966, 1155
FaeI CATG 2 cut(s) 51, 329
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 1 cut(s) 722
FatI CATG 2 cut(s) 47, 325
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 1501
FblI GTMKAC 1 cut(s) 509
Fnu4HI GCNGC 6 cut(s) 96, 583, 592, 615, 627, 1236
FriOI GRGCYC 1 cut(s) 1399
Fsp4HI GCNGC 6 cut(s) 96, 583, 592, 615, 627, 1236
FspBI CTAG 5 cut(s) 194, 236, 480, 720, 1340
GlaI GCGC 5 cut(s) 255, 287, 399, 535, 899
GluI GCNGC 6 cut(s) 96, 583, 592, 615, 627, 1236
GsuI CTGGAG 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 1 cut(s) 769
HapII CCGG 4 cut(s) 218, 387, 429, 1419
HhaI GCGC 5 cut(s) 256, 288, 400, 536, 900
Hin1II CATG 2 cut(s) 51, 329
Hin6I GCGC 5 cut(s) 254, 286, 398, 534, 898
HinP1I GCGC 5 cut(s) 254, 286, 398, 534, 898
HinfI GANTC 3 cut(s) 105, 231, 1354
HpaII CCGG 4 cut(s) 218, 387, 429, 1419
HphI GGTGA 4 cut(s) 157, 251, 1336, 1363
Hpy166II GTNNAC 4 cut(s) 510, 1027, 1299, 1463
Hpy188I TCNGA 4 cut(s) 206, 574, 1002, 1477
Hpy188III TCNNGA 4 cut(s) 248, 422, 806, 1492
Hpy8I GTNNAC 4 cut(s) 510, 1027, 1299, 1463
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 4 cut(s) 949, 1120, 1183, 1548
HpyCH4III ACNGT 3 cut(s) 763, 784, 888
HpyF10VI GCNNNNNNNGC 9 cut(s) 272, 540, 549, 588, 591, 623, 794, 904, 1241
HpyF3I CTNAG 9 cut(s) 318, 571, 645, 685, 749, 893, 908, 1001, 1521
Hsp92II CATG 2 cut(s) 51, 329
HspAI GCGC 5 cut(s) 254, 286, 398, 534, 898
KroI GCCGGC 1 cut(s) 386
KroNI GCCGGC 1 cut(s) 388
Kzo9I GATC 4 cut(s) 133, 407, 1151, 1203
LmnI GCTCC 2 cut(s) 531, 1394
Lsp1109I GCAGC 5 cut(s) 107, 603, 626, 638, 1247
LweI GCATC 4 cut(s) 241, 705, 799, 831
MaeI CTAG 5 cut(s) 194, 236, 480, 720, 1340
MaeIII GTNAC 4 cut(s) 306, 495, 1324, 1351
MalI GATC 4 cut(s) 135, 409, 1153, 1205
MboI GATC 4 cut(s) 133, 407, 1151, 1203
MboII GAAGA 4 cut(s) 55, 71, 1088, 1345
MfeI CAATTG 1 cut(s) 1386
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 1399
MlyI GAGTC 3 cut(s) 99, 240, 1348
MmeI TCCRAC 1 cut(s) 509
Mph1103I ATGCAT 1 cut(s) 1120
MroNI GCCGGC 1 cut(s) 386
MseI TTAA 2 cut(s) 1112, 1545
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 4 cut(s) 594, 629, 1238, 1445
MspI CCGG 4 cut(s) 218, 387, 429, 1419
MspR9I CCNGG 3 cut(s) 84, 218, 1401
MunI CAATTG 1 cut(s) 1386
Mva1269I GAATGC 1 cut(s) 330
MvaI CCWGG 2 cut(s) 84, 1401
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 9 cut(s) 272, 540, 549, 588, 591, 623, 794, 904, 1241
NaeI GCCGGC 1 cut(s) 388
NciI CCSGG 1 cut(s) 218
NdeI CATATG 1 cut(s) 1501
NdeII GATC 4 cut(s) 133, 407, 1151, 1203
NgoMIV GCCGGC 1 cut(s) 386
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 2 cut(s) 51, 329
NlaIV GGNNCC 2 cut(s) 300, 1396
NmuCI GTSAC 4 cut(s) 306, 495, 1324, 1351
NsiI ATGCAT 1 cut(s) 1120
OliI CACNNNNGTG 1 cut(s) 23
PaeR7I CTCGAG 1 cut(s) 169
PctI GAATGC 1 cut(s) 330
PdiI GCCGGC 1 cut(s) 388
PflMI CCANNNNNTGG 1 cut(s) 1195
PkrI GCNGC 6 cut(s) 97, 584, 593, 616, 628, 1237
PleI GAGTC 3 cut(s) 99, 239, 1348
PpsI GAGTC 3 cut(s) 99, 239, 1348
PsiI TTATAA 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 82, 1399
PspGI CCWGG 2 cut(s) 82, 1399
PspN4I GGNNCC 2 cut(s) 300, 1396
PspPI GGNCC 2 cut(s) 291, 767
PspXI VCTCGAGB 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 1195
PsuI RGATCY 1 cut(s) 407
PvuII CAGCTG 2 cut(s) 1238, 1445
RsaI GTAC 1 cut(s) 863
RsaNI GTAC 1 cut(s) 862
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 2 cut(s) 1112, 1545
SatI GCNGC 6 cut(s) 96, 583, 592, 615, 627, 1236
Sau3AI GATC 4 cut(s) 133, 407, 1151, 1203
Sau96I GGNCC 2 cut(s) 291, 767
SchI GAGTC 3 cut(s) 99, 240, 1348
ScrFI CCNGG 3 cut(s) 84, 218, 1401
SduI GDGCHC 1 cut(s) 1399
SfaNI GCATC 4 cut(s) 241, 705, 799, 831
Sfr274I CTCGAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 3 cut(s) 169, 484, 586
SmoI CTYRAG 3 cut(s) 169, 484, 586
SpeI ACTAGT 1 cut(s) 719
SsiI CCGC 8 cut(s) 100, 384, 448, 543, 582, 594, 629, 1011
SspI AATATT 1 cut(s) 188
SspMI CTAG 5 cut(s) 194, 236, 480, 720, 1340
StyD4I CCNGG 3 cut(s) 82, 216, 1399
StyI CCWWGG 4 cut(s) 294, 363, 966, 1155
TaaI ACNGT 3 cut(s) 763, 784, 888
TaqI TCGA 4 cut(s) 170, 724, 825, 1491
TaqII GACCGA 2 cut(s) 701, 1067
TatI WGTACW 1 cut(s) 861
TauI GCSGC 1 cut(s) 585
Tru1I TTAA 2 cut(s) 1112, 1545
Tru9I TTAA 2 cut(s) 1112, 1545
TscAI CASTG 6 cut(s) 25, 309, 615, 649, 967, 1152
TseFI GTSAC 4 cut(s) 306, 495, 1324, 1351
TseI GCWGC 5 cut(s) 95, 591, 614, 626, 1235
Tsp45I GTSAC 4 cut(s) 306, 495, 1324, 1351
TspDTI ATGAA 4 cut(s) 389, 624, 1089, 1530
TspRI CASTG 6 cut(s) 25, 309, 615, 649, 967, 1152
Van91I CCANNNNNTGG 1 cut(s) 1195
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 2 cut(s) 32, 1285
XhoI CTCGAG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 509
XspI CTAG 5 cut(s) 194, 236, 480, 720, 1340
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.