Rh6DG020400

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000427
Physical Location & Seq
Reverse (-)
1811419 .. 1820112
8694 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG020400.1

Sequence Viewer

Length: 1590 bp
ATGATAATTTCCTCATCCACTGCCATGGCGAAATTCCCTCACATCTCATGCTTATTGCTTCTCTTCACATTGTCATCTCTTCCAGGGACATTCGCAGCCCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTCAAGCACAACCAACTCACCTCGAGGCAACTTGTTCAATATTACCTTGCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAACCCGGATGCGCTTCAGCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCGCTGCTTGGATCTGTCGTGCCTCGTGATGCCGGTGTGGTGGAGAAGTTGAGGAAAGCAGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGTCTAATGATGAACCCTCTGATTGGAGCGCCAGAGGCGGTTATGGCTTGAGCCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTGTCATTAGGTACCGAGACAAGTGGTTCAATCTTATGTCCATCAAGTTTGAACTCAGTTGTGGGCCTCAGACCAACAGTCGGTCTTACTAGTCGAGCAGGGGTTGTGCCAATTTCTCTAAGACAGGATACTGTTGGGCCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGGATCGACCACAATGACATTGCAACAATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGTGGAAAAAGAATAGGGATATTGGGAGCCTTCCATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGAGCTGTTTTGGTAGATAATTTGGAAATTGCTAATTTGACTGAAATCTATTCTAGAAGAGATGAATATGCTGCATTGTATAATGAGTGCAAAATAACCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCGGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCAGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACCGATTTTTAGCAGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCCGGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTGAAATTGGTGACAGCGAAGAAGCTAGATGCTGTGGTGACTCCTAACGCAGCTTTTTCAAGGGTACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCCGGATACGAAAACAATGGGAGACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCCACTAAGATTCCTTCGAGTTTTAGAACAACATTGTATTACTTGGAGAATGCAGTGTGCTATGAGAAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

529

Amino Acids

56.94

Weight (kDa)

9.24

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 60 - 455 4.5e-68 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 169
Acc65I GGTACC 1 cut(s) 641
AccB1I GGYRCC 2 cut(s) 298, 641
AccB7I CCANNNNNTGG 1 cut(s) 1195
AccII CGCG 1 cut(s) 900
AciI CCGC 6 cut(s) 100, 384, 543, 582, 594, 629
AclWI GGATC 3 cut(s) 415, 830, 1146
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 2 cut(s) 242, 1260
AfaI GTAC 3 cut(s) 643, 863, 1380
AfiI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
AgsI TTSAA 8 cut(s) 151, 185, 660, 682, 1126, 1177, 1285, 1374
AhdI GACNNNNNGTC 1 cut(s) 707
AhlI ACTAGT 1 cut(s) 719
AjnI CCWGG 2 cut(s) 82, 1399
Alw26I GTCTC 3 cut(s) 641, 845, 1432
AlwI GGATC 3 cut(s) 415, 830, 1146
AlwNI CAGNNNCTG 2 cut(s) 794, 1195
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 2 cut(s) 694, 767
ApeKI GCWGC 9 cut(s) 95, 400, 591, 614, 626, 794, 1082, 1235, 1364
ApoI RAATTY 1 cut(s) 32
Asp718I GGTACC 1 cut(s) 641
AspLEI GCGC 5 cut(s) 256, 288, 400, 536, 900
AspS9I GGNCC 3 cut(s) 291, 694, 767
AsuC2I CCSGG 3 cut(s) 218, 248, 1145
AsuHPI GGTGA 3 cut(s) 157, 1336, 1363
AsuNHI GCTAGC 1 cut(s) 479
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 291
BanI GGYRCC 2 cut(s) 298, 641
BanII GRGCYC 2 cut(s) 560, 1399
BauI CACGAG 1 cut(s) 420
BbvI GCAGC 9 cut(s) 107, 387, 603, 626, 638, 781, 1069, 1247, 1376
BccI CCATC 1 cut(s) 679
BciT130I CCWGG 2 cut(s) 84, 1401
BciVI GTATCC 2 cut(s) 751, 1415
BcnI CCSGG 3 cut(s) 218, 248, 1145
BcoDI GTCTC 3 cut(s) 641, 845, 1432
BcuI ACTAGT 1 cut(s) 719
BfaI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1588
BfoI RGCGCY 1 cut(s) 537
BfuI GTATCC 2 cut(s) 751, 1415
BglI GCCNNNNNGGC 1 cut(s) 272
Bme1390I CCNGG 5 cut(s) 84, 218, 248, 1145, 1401
Bme18I GGWCC 1 cut(s) 291
BmeRI GACNNNNNGTC 1 cut(s) 707
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 291, 694, 767
BmiI GGNNCC 4 cut(s) 300, 643, 937, 1396
BmrFI CCNGG 5 cut(s) 84, 218, 248, 1145, 1401
BmsI GCATC 5 cut(s) 241, 415, 781, 799, 1333
BmtI GCTAGC 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 122
BpuEI CTTGAG 3 cut(s) 505, 571, 574
BpuMI CCSGG 3 cut(s) 218, 248, 1145
BsaI GGTCTC 1 cut(s) 1432
BsaJI CCNNGG 8 cut(s) 24, 83, 294, 363, 966, 1143, 1155, 1399
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
Bse118I RCCGGY 3 cut(s) 386, 428, 1273
Bse1I ACTGG 2 cut(s) 302, 1200
Bse3DI GCAATG 3 cut(s) 354, 837, 961
BseBI CCWGG 2 cut(s) 84, 1401
BseDI CCNNGG 8 cut(s) 24, 83, 294, 363, 966, 1143, 1155, 1399
BseGI GGATG 2 cut(s) 14, 256
BseLI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
BseMI GCAATG 3 cut(s) 354, 837, 961
BseMII CTCAG 5 cut(s) 585, 699, 712, 907, 992
BseNI ACTGG 2 cut(s) 302, 1200
BseRI GAGGAG 1 cut(s) 1026
BseXI GCAGC 9 cut(s) 107, 387, 603, 626, 638, 781, 1069, 1247, 1376
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 2 cut(s) 696, 769
BshNI GGYRCC 2 cut(s) 298, 641
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 7 cut(s) 218, 248, 387, 429, 1145, 1274, 1419
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
BsmAI GTCTC 3 cut(s) 641, 845, 1432
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 2 cut(s) 330, 1570
BsnI GGCC 2 cut(s) 696, 769
Bso31I GGTCTC 1 cut(s) 1432
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 560, 1399
Bsp143I GATC 5 cut(s) 133, 407, 822, 1151, 1203
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 6 cut(s) 100, 384, 543, 582, 594, 629
BspANI GGCC 2 cut(s) 696, 769
BspCNI CTCAG 5 cut(s) 584, 698, 711, 906, 993
BspFNI CGCG 1 cut(s) 900
BspLI GGNNCC 4 cut(s) 300, 643, 937, 1396
BspOI GCTAGC 1 cut(s) 483
BspPI GGATC 3 cut(s) 415, 830, 1146
BspQI GCTCTTC 1 cut(s) 1577
BspT107I GGYRCC 2 cut(s) 298, 641
BspTNI GGTCTC 1 cut(s) 1432
BsrDI GCAATG 3 cut(s) 354, 837, 961
BsrFI RCCGGY 3 cut(s) 386, 428, 1273
BsrI ACTGG 2 cut(s) 302, 1200
BssAI RCCGGY 3 cut(s) 386, 428, 1273
BssECI CCNNGG 8 cut(s) 24, 83, 294, 363, 966, 1143, 1155, 1399
BssMI GATC 5 cut(s) 133, 407, 822, 1151, 1203
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 2 cut(s) 84, 1401
Bst4CI ACNGT 4 cut(s) 709, 763, 784, 888
Bst6I CTCTTC 4 cut(s) 68, 84, 1063, 1577
BstAPI GCANNNNNTGC 1 cut(s) 794
BstC8I GCNNGC 7 cut(s) 100, 144, 264, 388, 481, 1275, 1515
BstDEI CTNAG 9 cut(s) 318, 571, 685, 698, 749, 893, 908, 1001, 1521
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 14, 256
BstFNI CGCG 1 cut(s) 900
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 5 cut(s) 256, 288, 400, 536, 900
BstKTI GATC 5 cut(s) 136, 410, 825, 1154, 1206
BstMAI GTCTC 3 cut(s) 641, 845, 1432
BstMBI GATC 5 cut(s) 133, 407, 822, 1151, 1203
BstMWI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
BstNI CCWGG 2 cut(s) 84, 1401
BstSCI CCNGG 5 cut(s) 82, 216, 246, 1143, 1399
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 9 cut(s) 107, 387, 603, 626, 638, 781, 1069, 1247, 1376
BstX2I RGATCY 1 cut(s) 407
BstXI CCANNNNNNTGG 2 cut(s) 25, 950
BstYI RGATCY 1 cut(s) 407
BsuI GTATCC 2 cut(s) 751, 1415
BsuRI GGCC 2 cut(s) 696, 769
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 2 cut(s) 14, 256
BtsI GCAGTG 2 cut(s) 18, 1575
BtsIMutI CAGTG 4 cut(s) 18, 309, 615, 1575
Cac8I GCNNGC 7 cut(s) 100, 144, 264, 388, 481, 1275, 1515
CaiI CAGNNNCTG 2 cut(s) 794, 1195
CfoI GCGC 5 cut(s) 256, 288, 400, 536, 900
Cfr10I RCCGGY 3 cut(s) 386, 428, 1273
Cfr13I GGNCC 3 cut(s) 291, 694, 767
Csp6I GTAC 3 cut(s) 642, 862, 1379
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 4 cut(s) 25, 48, 326, 944
CviQI GTAC 3 cut(s) 642, 862, 1379
DdeI CTNAG 9 cut(s) 318, 571, 685, 698, 749, 893, 908, 1001, 1521
DpnI GATC 5 cut(s) 135, 409, 824, 1153, 1205
DpnII GATC 5 cut(s) 133, 407, 822, 1151, 1203
DriI GACNNNNNGTC 1 cut(s) 707
Eam1104I CTCTTC 4 cut(s) 68, 84, 1063, 1577
Eam1105I GACNNNNNGTC 1 cut(s) 707
EarI CTCTTC 4 cut(s) 68, 84, 1063, 1577
Eco130I CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
Eco24I GRGCYC 2 cut(s) 560, 1399
Eco31I GGTCTC 1 cut(s) 1432
Eco47I GGWCC 1 cut(s) 291
Eco57I CTGAAG 2 cut(s) 242, 1260
Eco88I CYCGRG 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 82, 1399
EcoT14I CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
EcoT22I ATGCAT 1 cut(s) 1120
EcoT38I GRGCYC 2 cut(s) 560, 1399
ErhI CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
FaeI CATG 4 cut(s) 28, 51, 329, 947
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 1 cut(s) 100
FatI CATG 4 cut(s) 24, 47, 325, 943
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 1501
FokI GGATG 1 cut(s) 263
FriOI GRGCYC 2 cut(s) 560, 1399
FspBI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1588
GlaI GCGC 5 cut(s) 255, 287, 399, 535, 899
GsuI CTGGAG 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 2 cut(s) 696, 769
HapII CCGG 7 cut(s) 218, 248, 387, 429, 1145, 1274, 1419
HhaI GCGC 5 cut(s) 256, 288, 400, 536, 900
Hin1II CATG 4 cut(s) 28, 51, 329, 947
Hin6I GCGC 5 cut(s) 254, 286, 398, 534, 898
HinP1I GCGC 5 cut(s) 254, 286, 398, 534, 898
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 5 cut(s) 105, 231, 1285, 1354, 1525
HpaII CCGG 7 cut(s) 218, 248, 387, 429, 1145, 1274, 1419
HphI GGTGA 3 cut(s) 157, 1336, 1363
Hpy166II GTNNAC 3 cut(s) 244, 1299, 1463
Hpy188I TCNGA 6 cut(s) 206, 526, 574, 701, 1002, 1477
Hpy188III TCNNGA 4 cut(s) 422, 806, 1065, 1492
Hpy8I GTNNAC 3 cut(s) 244, 1299, 1463
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 4 cut(s) 949, 1120, 1183, 1539
HpyCH4III ACNGT 4 cut(s) 709, 763, 784, 888
HpyF10VI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
HpyF3I CTNAG 9 cut(s) 318, 571, 685, 698, 749, 893, 908, 1001, 1521
Hsp92II CATG 4 cut(s) 28, 51, 329, 947
HspAI GCGC 5 cut(s) 254, 286, 398, 534, 898
KpnI GGTACC 1 cut(s) 645
KroI GCCGGC 2 cut(s) 386, 1273
KroNI GCCGGC 2 cut(s) 388, 1275
Kzo9I GATC 5 cut(s) 133, 407, 822, 1151, 1203
LguI GCTCTTC 1 cut(s) 1577
LmnI GCTCC 4 cut(s) 531, 935, 1013, 1394
Lsp1109I GCAGC 9 cut(s) 107, 387, 603, 626, 638, 781, 1069, 1247, 1376
LweI GCATC 5 cut(s) 241, 415, 781, 799, 1333
MaeI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1588
MaeIII GTNAC 4 cut(s) 306, 495, 1324, 1351
MalI GATC 5 cut(s) 135, 409, 824, 1153, 1205
MboI GATC 5 cut(s) 133, 407, 822, 1151, 1203
MboII GAAGA 4 cut(s) 55, 71, 1080, 1345
MfeI CAATTG 2 cut(s) 846, 1386
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 2 cut(s) 560, 1399
MlyI GAGTC 3 cut(s) 99, 240, 1348
Mph1103I ATGCAT 1 cut(s) 1120
MroNI GCCGGC 2 cut(s) 386, 1273
MseI TTAA 1 cut(s) 1112
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 4 cut(s) 594, 629, 1238, 1445
MspI CCGG 7 cut(s) 218, 248, 387, 429, 1145, 1274, 1419
MspR9I CCNGG 5 cut(s) 84, 218, 248, 1145, 1401
MunI CAATTG 2 cut(s) 846, 1386
Mva1269I GAATGC 2 cut(s) 330, 1570
MvaI CCWGG 2 cut(s) 84, 1401
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
NaeI GCCGGC 2 cut(s) 388, 1275
NciI CCSGG 3 cut(s) 218, 248, 1145
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 1501
NdeII GATC 5 cut(s) 133, 407, 822, 1151, 1203
NgoMIV GCCGGC 2 cut(s) 386, 1273
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 4 cut(s) 28, 51, 329, 947
NlaIV GGNNCC 4 cut(s) 300, 643, 937, 1396
NmuCI GTSAC 4 cut(s) 306, 495, 1324, 1351
NsiI ATGCAT 1 cut(s) 1120
PaeR7I CTCGAG 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 1577
PctI GAATGC 2 cut(s) 330, 1570
PdiI GCCGGC 2 cut(s) 388, 1275
PfeI GAWTC 2 cut(s) 1285, 1525
PflMI CCANNNNNTGG 1 cut(s) 1195
PleI GAGTC 3 cut(s) 99, 239, 1348
PpsI GAGTC 3 cut(s) 99, 239, 1348
Psp6I CCWGG 2 cut(s) 82, 1399
PspGI CCWGG 2 cut(s) 82, 1399
PspN4I GGNNCC 4 cut(s) 300, 643, 937, 1396
PspPI GGNCC 3 cut(s) 291, 694, 767
PspXI VCTCGAGB 1 cut(s) 169
PstNI CAGNNNCTG 2 cut(s) 794, 1195
PsuI RGATCY 1 cut(s) 407
PvuII CAGCTG 2 cut(s) 1238, 1445
RsaI GTAC 3 cut(s) 643, 863, 1380
RsaNI GTAC 3 cut(s) 642, 862, 1379
RseI CAYNNNNRTG 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 1577
SaqAI TTAA 1 cut(s) 1112
Sau3AI GATC 5 cut(s) 133, 407, 822, 1151, 1203
Sau96I GGNCC 3 cut(s) 291, 694, 767
SchI GAGTC 3 cut(s) 99, 240, 1348
ScrFI CCNGG 5 cut(s) 84, 218, 248, 1145, 1401
SduI GDGCHC 2 cut(s) 560, 1399
SfaNI GCATC 5 cut(s) 241, 415, 781, 799, 1333
Sfr274I CTCGAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 4 cut(s) 169, 484, 553, 586
SmoI CTYRAG 4 cut(s) 169, 484, 553, 586
SpeI ACTAGT 1 cut(s) 719
SsiI CCGC 6 cut(s) 100, 384, 543, 582, 594, 629
SspI AATATT 1 cut(s) 188
SspMI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1588
StyD4I CCNGG 5 cut(s) 82, 216, 246, 1143, 1399
StyI CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
TaaI ACNGT 4 cut(s) 709, 763, 784, 888
TaqI TCGA 5 cut(s) 170, 724, 825, 1491, 1532
TaqII GACCGA 2 cut(s) 701, 1239
TatI WGTACW 1 cut(s) 861
TauI GCSGC 1 cut(s) 585
TfiI GAWTC 2 cut(s) 1285, 1525
Tru1I TTAA 1 cut(s) 1112
Tru9I TTAA 1 cut(s) 1112
TscAI CASTG 4 cut(s) 25, 309, 615, 1575
TseFI GTSAC 4 cut(s) 306, 495, 1324, 1351
TseI GCWGC 9 cut(s) 95, 400, 591, 614, 626, 794, 1082, 1235, 1364
Tsp45I GTSAC 4 cut(s) 306, 495, 1324, 1351
TspDTI ATGAA 3 cut(s) 389, 531, 1089
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 4 cut(s) 25, 309, 615, 1575
Van91I CCANNNNNTGG 1 cut(s) 1195
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 1 cut(s) 32
XbaI TCTAGA 1 cut(s) 1064
XhoI CTCGAG 1 cut(s) 169
XspI CTAG 6 cut(s) 236, 480, 720, 1065, 1340, 1588
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.