MD05G1117200.v1.1

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000427
Physical Location & Seq
Forward (+)
23811216 .. 23813724
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1117200.v1.1.491

Sequence Viewer

Length: 1539 bp
ATGGCCACAAAACTTCCGTTGTTGCTCGTGATTCTTCTGTCTTTAACTTCATTTGGTTCCCAAACTTTGTATGTCCATGGGTTCTATATTAAAGAAGCTACAATCTCTGACCTCCAACAAGCTTTCAAACAAAACCAACTCACTTCAAGAAAACTTGTTGAGTTCTACCTTCAAGAAATCCGCCGGCTCAACCCTCTCCTCAGCGCCGTCATAGAGGTCAACCCGGACGTGCTTTACCAAGCTGATAAGGCCGACTATCAGCGAAAGAGTAAGGGCCCCAATTATTACTCTGGGTTGCATGGCATTCCGGTTTTGCTCAAGGACAACATTGGAACGAAAGATAAGCTGAACACGACTGCTGGCTCGTTTGCGCTGCTTGGTTCCGTGGTGCCTCGGGATGCTGGCGTGGTGACCAGGTTGAGGAATGCCGGTGCCATCATATTGGGGAAGGCTAGCTTGAGTGAGTGGGCTCACTTTAGGTCTCTTGCAGCACCTGCTGGTTGGAGTCCCAGAGGTGGCCAAGGAAAGAACCCTTATGTGCTATCAGCAACTCCATGTGGCTCAAGCAGTGGACCTGCAATTTCAGCTGCGGCAAATTTGGTAGCAGTAACTCTGGGGACTGAGACTGATGGCTCAATACTTTGTCCGGCAAGTTTCAACTCTGTAGTGGGCATCAAACCAACCGTTGGTCTCACCAGCCGTGCTGGGGTGATCCCGGTAACTCCCAGACAGGACACCATTGGACCCATCACAAGGACAGTGTCAGACGCGGTTGATGTTCTTGACGCCATTGTAGGCTATGACTACAACGATCAAGCAACCCGAGAGGCGTCCAAGTACATTCCGCGTGGTGGCTATAAACAATTCCTTCAGAGATATGGACTCAAAGGTAAGAGATTGGGAATCGTCAGGAATCCGTTCTTCACTTCTGGCAGCGGATCTCTCCAACTTAAATCGTTTGAGAAACATTTCAAGACTCTAAGGCAAGAAGGTGCTGTATTGGTAGACCATCTAGAAATAGCTAACATTGATGTTATATTGAACTTCAATTTGAGTGGGGAAGCCATAGCTTCACTGGCTGAGTTCAAACTAGCCTTGAATGCGTATTTAAAAGACCTAGTAGTATCTCCGGCTCGATCTTTAGCAGATGTAATTGCCTTCAACCTCAAGTTCTCGGGTGTGGAAATGATCAAGGAATTTGGGCAAGATATTTTTCTTGCAGCCCAAGCAACAAATGGGATAGGTAACAATGAAAAGGCAGCATTGTTGAATTTGGCAAAGCTGACAAAGGATGGATTTGAGAAATTGTTGAAGGACAACAGGTTAGATTCATTGGTGACTCCTGGTTCAGATGTTTCACCCGTGCTTGCAATTGGGGGATTCCCAGGAATCAGTGTCCCTGCTGGGTTTGACAAGAAGGGTGTACCTTTTGGGATAACATTTGGGGGACTGAAGGGTTCTGAGCCAAAGCTTATACAGATTGCCTATGGCTTTGAGCAAGCTACTAAAGTTAGAAAGGCTCCTACATTTCTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

513

Amino Acids

54.79

Weight (kDa)

9.47

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 52 - 448 1.4e-68 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 502
Acc36I ACCTGC 2 cut(s) 502, 583
AccB1I GGYRCC 2 cut(s) 388, 431
AccB7I CCANNNNNTGG 1 cut(s) 686
AccI GTMKAC 1 cut(s) 1005
AccII CGCG 2 cut(s) 770, 847
AciI CCGC 5 cut(s) 181, 590, 770, 845, 936
AclWI GGATC 2 cut(s) 706, 946
AcoI YGGCCR 2 cut(s) 3, 517
AcsI RAATTY 3 cut(s) 595, 1196, 1270
AcuI CTGAAG 2 cut(s) 854, 1472
AcyI GRCGYC 2 cut(s) 786, 830
AfaI GTAC 2 cut(s) 839, 1425
AfiI CCNNNNNNNGG 6 cut(s) 420, 515, 686, 706, 753, 851
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 3 cut(s) 413, 1342, 1384
Alw26I GTCTC 3 cut(s) 486, 617, 695
AlwI GGATC 2 cut(s) 706, 946
AlwNI CAGNNNCTG 1 cut(s) 494
Ama87I CYCGRG 3 cut(s) 393, 822, 1174
AoxI GGCC 4 cut(s) 3, 249, 274, 517
ApaI GGGCCC 1 cut(s) 278
ApeKI GCWGC 6 cut(s) 373, 488, 587, 933, 1220, 1259
ApoI RAATTY 3 cut(s) 595, 1196, 1270
Asp700I GAANNNNTTC 2 cut(s) 917, 968
AspLEI GCGC 2 cut(s) 206, 373
AspS9I GGNCC 4 cut(s) 274, 275, 572, 743
AsuC2I CCSGG 2 cut(s) 224, 716
AsuHPI GGTGA 5 cut(s) 421, 685, 721, 1348, 1350
AsuNHI GCTAGC 1 cut(s) 452
AvaI CYCGRG 3 cut(s) 393, 822, 1174
AvaII GGWCC 2 cut(s) 572, 743
BaeGI GKGCMC 1 cut(s) 278
BalI TGGCCA 2 cut(s) 5, 519
BanI GGYRCC 2 cut(s) 388, 431
BanII GRGCYC 2 cut(s) 278, 472
BauI CACGAG 1 cut(s) 26
BbvCI CCTCAGC 1 cut(s) 200
BbvI GCAGC 6 cut(s) 360, 500, 574, 945, 1232, 1271
BccI CCATC 5 cut(s) 443, 623, 755, 1017, 1286
BceAI ACGGC 2 cut(s) 191, 684
BciT130I CCWGG 3 cut(s) 415, 1344, 1386
BclI TGATCA 1 cut(s) 1188
BcnI CCSGG 2 cut(s) 224, 716
BcoDI GTCTC 3 cut(s) 486, 617, 695
BfaI CTAG 4 cut(s) 453, 1013, 1091, 1118
BfmI CTRYAG 1 cut(s) 663
BfoI RGCGCY 1 cut(s) 207
BfuAI ACCTGC 2 cut(s) 502, 583
BisI GCNGC 7 cut(s) 374, 489, 588, 591, 934, 1221, 1260
BlsI GCNGC 7 cut(s) 375, 490, 589, 592, 935, 1222, 1261
Bme1390I CCNGG 5 cut(s) 224, 415, 716, 1344, 1386
Bme18I GGWCC 2 cut(s) 572, 743
BmeT110I CYCGRG 3 cut(s) 393, 822, 1174
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 4 cut(s) 274, 275, 572, 743
BmiI GGNNCC 8 cut(s) 58, 276, 277, 382, 390, 433, 745, 1521
BmrFI CCNGG 5 cut(s) 224, 415, 716, 1344, 1386
BmsI GCATC 2 cut(s) 388, 681
BmtI GCTAGC 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 200
BpuEI CTTGAG 4 cut(s) 302, 478, 547, 1151
BpuMI CCSGG 2 cut(s) 224, 716
BsaHI GRCGYC 2 cut(s) 786, 830
BsaI GGTCTC 2 cut(s) 486, 695
BsaJI CCNNGG 5 cut(s) 76, 384, 392, 520, 1384
BsaWI WCCGGW 1 cut(s) 307
Bsc4I CCNNNNNNNGG 6 cut(s) 420, 515, 686, 706, 753, 851
Bse118I RCCGGY 2 cut(s) 183, 428
Bse1I ACTGG 1 cut(s) 1080
BseBI CCWGG 3 cut(s) 415, 1344, 1386
BseDI CCNNGG 5 cut(s) 76, 384, 392, 520, 1384
BseGI GGATG 2 cut(s) 403, 1297
BseLI CCNNNNNNNGG 6 cut(s) 420, 515, 686, 706, 753, 851
BseMII CTCAG 4 cut(s) 214, 612, 1071, 1452
BseNI ACTGG 1 cut(s) 1080
BseRI GAGGAG 1 cut(s) 188
BseSI GKGCMC 1 cut(s) 278
BseXI GCAGC 6 cut(s) 360, 500, 574, 945, 1232, 1271
BseYI CCCAGC 2 cut(s) 704, 1403
Bsh1236I CGCG 2 cut(s) 770, 847
BshFI GGCC 4 cut(s) 5, 251, 276, 519
BshNI GGYRCC 2 cut(s) 388, 431
BsiHKCI CYCGRG 3 cut(s) 393, 822, 1174
BsiSI CCGG 7 cut(s) 184, 224, 308, 429, 647, 716, 1130
BslFI GGGAC 4 cut(s) 492, 631, 1382, 1461
BslI CCNNNNNNNGG 6 cut(s) 420, 515, 686, 706, 753, 851
BsmAI GTCTC 3 cut(s) 486, 617, 695
BsmFI GGGAC 4 cut(s) 492, 631, 1382, 1461
BsmI GAATGC 3 cut(s) 303, 430, 1105
BsnI GGCC 4 cut(s) 5, 251, 276, 519
Bso31I GGTCTC 2 cut(s) 486, 695
BsoBI CYCGRG 3 cut(s) 393, 822, 1174
Bsp120I GGGCCC 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 278, 472
Bsp143I GATC 5 cut(s) 711, 811, 938, 1136, 1188
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 5 cut(s) 181, 590, 770, 845, 936
BspANI GGCC 4 cut(s) 5, 251, 276, 519
BspCNI CTCAG 4 cut(s) 213, 613, 1072, 1453
BspFNI CGCG 2 cut(s) 770, 847
BspLI GGNNCC 8 cut(s) 58, 276, 277, 382, 390, 433, 745, 1521
BspMI ACCTGC 2 cut(s) 502, 583
BspOI GCTAGC 1 cut(s) 456
BspPI GGATC 2 cut(s) 706, 946
BspT107I GGYRCC 2 cut(s) 388, 431
BspTNI GGTCTC 2 cut(s) 486, 695
BsrFI RCCGGY 2 cut(s) 183, 428
BsrI ACTGG 1 cut(s) 1080
BssAI RCCGGY 2 cut(s) 183, 428
BssECI CCNNGG 5 cut(s) 76, 384, 392, 520, 1384
BssMI GATC 5 cut(s) 711, 811, 938, 1136, 1188
BssNI GRCGYC 2 cut(s) 786, 830
BssSI CACGAG 1 cut(s) 26
BssT1I CCWWGG 2 cut(s) 76, 520
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 3 cut(s) 415, 1344, 1386
Bst4CI ACNGT 2 cut(s) 685, 760
BstACI GRCGYC 2 cut(s) 786, 830
BstAPI GCANNNNNTGC 1 cut(s) 494
BstC8I GCNNGC 6 cut(s) 185, 361, 403, 454, 1368, 1500
BstDEI CTNAG 5 cut(s) 200, 621, 980, 1080, 1461
BstDSI CCRYGG 2 cut(s) 76, 384
BstEII GGTNACC 1 cut(s) 409
BstF5I GGATG 2 cut(s) 403, 1297
BstFNI CGCG 2 cut(s) 770, 847
BstH2I RGCGCY 1 cut(s) 207
BstHHI GCGC 2 cut(s) 206, 373
BstKTI GATC 5 cut(s) 714, 814, 941, 1139, 1191
BstMAI GTCTC 3 cut(s) 486, 617, 695
BstMBI GATC 5 cut(s) 711, 811, 938, 1136, 1188
BstMWI GCNNNNNNNGC 6 cut(s) 248, 494, 584, 1076, 1100, 1226
BstNI CCWGG 3 cut(s) 415, 1344, 1386
BstPI GGTNACC 1 cut(s) 409
BstSCI CCNGG 5 cut(s) 222, 413, 714, 1342, 1384
BstSFI CTRYAG 1 cut(s) 663
BstSLI GKGCMC 1 cut(s) 278
BstUI CGCG 2 cut(s) 770, 847
BstV1I GCAGC 6 cut(s) 360, 500, 574, 945, 1232, 1271
BstX2I RGATCY 1 cut(s) 938
BstXI CCANNNNNNTGG 1 cut(s) 442
BstYI RGATCY 1 cut(s) 938
BsuRI GGCC 4 cut(s) 5, 251, 276, 519
BtgI CCRYGG 2 cut(s) 76, 384
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 2 cut(s) 403, 1297
BtsI GCAGTG 1 cut(s) 574
BtsIMutI CAGTG 4 cut(s) 574, 765, 1073, 1399
BveI ACCTGC 2 cut(s) 502, 583
Cac8I GCNNGC 6 cut(s) 185, 361, 403, 454, 1368, 1500
CaiI CAGNNNCTG 1 cut(s) 494
CfoI GCGC 2 cut(s) 206, 373
Cfr10I RCCGGY 2 cut(s) 183, 428
Cfr13I GGNCC 4 cut(s) 274, 275, 572, 743
CseI GACGC 3 cut(s) 776, 794, 819
CsiI ACCWGGT 1 cut(s) 413
Csp6I GTAC 2 cut(s) 838, 1424
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviAII CATG 3 cut(s) 77, 299, 555
CviQI GTAC 2 cut(s) 838, 1424
DdeI CTNAG 5 cut(s) 200, 621, 980, 1080, 1461
DpnI GATC 5 cut(s) 713, 813, 940, 1138, 1190
DpnII GATC 5 cut(s) 711, 811, 938, 1136, 1188
DraI TTTAAA 1 cut(s) 1110
EaeI YGGCCR 2 cut(s) 3, 517
EciI GGCGGA 1 cut(s) 170
Eco130I CCWWGG 2 cut(s) 76, 520
Eco24I GRGCYC 2 cut(s) 278, 472
Eco31I GGTCTC 2 cut(s) 486, 695
Eco47I GGWCC 2 cut(s) 572, 743
Eco57I CTGAAG 2 cut(s) 854, 1472
Eco88I CYCGRG 3 cut(s) 393, 822, 1174
Eco91I GGTNACC 1 cut(s) 409
EcoO109I RGGNCCY 2 cut(s) 274, 275
EcoO65I GGTNACC 1 cut(s) 409
EcoRII CCWGG 3 cut(s) 413, 1342, 1384
EcoT14I CCWWGG 2 cut(s) 76, 520
EcoT38I GRGCYC 2 cut(s) 278, 472
ErhI CCWWGG 2 cut(s) 76, 520
FaeI CATG 3 cut(s) 80, 302, 558
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 4 cut(s) 492, 631, 1382, 1461
FatI CATG 3 cut(s) 76, 298, 554
FbaI TGATCA 1 cut(s) 1188
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 7 cut(s) 374, 489, 588, 591, 934, 1221, 1260
FokI GGATG 2 cut(s) 410, 1304
FriOI GRGCYC 2 cut(s) 278, 472
Fsp4HI GCNGC 7 cut(s) 374, 489, 588, 591, 934, 1221, 1260
FspBI CTAG 4 cut(s) 453, 1013, 1091, 1118
GlaI GCGC 2 cut(s) 205, 372
GluI GCNGC 7 cut(s) 374, 489, 588, 591, 934, 1221, 1260
GsaI CCCAGC 2 cut(s) 708, 1407
HaeII RGCGCY 1 cut(s) 207
HaeIII GGCC 4 cut(s) 5, 251, 276, 519
HapII CCGG 7 cut(s) 184, 224, 308, 429, 647, 716, 1130
HgaI GACGC 3 cut(s) 776, 794, 819
HhaI GCGC 2 cut(s) 206, 373
Hin1I GRCGYC 2 cut(s) 786, 830
Hin1II CATG 3 cut(s) 80, 302, 558
Hin6I GCGC 2 cut(s) 204, 371
HinP1I GCGC 2 cut(s) 204, 371
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HindIII AAGCTT 2 cut(s) 120, 1469
HpaII CCGG 7 cut(s) 184, 224, 308, 429, 647, 716, 1130
HphI GGTGA 5 cut(s) 421, 685, 721, 1348, 1350
Hpy166II GTNNAC 4 cut(s) 220, 572, 1006, 1424
Hpy188I TCNGA 5 cut(s) 109, 766, 873, 1351, 1462
Hpy188III TCNNGA 8 cut(s) 28, 147, 173, 395, 782, 910, 973, 1013
Hpy8I GTNNAC 4 cut(s) 220, 572, 1006, 1424
HpyAV CCTTC 8 cut(s) 179, 442, 878, 983, 1168, 1306, 1411, 1447
HpyCH4III ACNGT 2 cut(s) 685, 760
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 5 cut(s) 298, 488, 578, 1220, 1370
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 494, 584, 1076, 1100, 1226
HpyF3I CTNAG 5 cut(s) 200, 621, 980, 1080, 1461
HpySE526I ACGT 1 cut(s) 228
Hsp92I GRCGYC 2 cut(s) 786, 830
Hsp92II CATG 3 cut(s) 80, 302, 558
HspAI GCGC 2 cut(s) 204, 371
KroI GCCGGC 1 cut(s) 183
KroNI GCCGGC 1 cut(s) 185
Ksp22I TGATCA 1 cut(s) 1188
Kzo9I GATC 5 cut(s) 711, 811, 938, 1136, 1188
LmnI GCTCC 1 cut(s) 1525
Lsp1109I GCAGC 6 cut(s) 360, 500, 574, 945, 1232, 1271
LweI GCATC 2 cut(s) 388, 681
MabI ACCWGGT 1 cut(s) 413
MaeI CTAG 4 cut(s) 453, 1013, 1091, 1118
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 5 cut(s) 409, 607, 718, 1244, 1336
MalI GATC 5 cut(s) 713, 813, 940, 1138, 1190
MboI GATC 5 cut(s) 711, 811, 938, 1136, 1188
MboII GAAGA 2 cut(s) 26, 913
MfeI CAATTG 1 cut(s) 1371
MflI RGATCY 1 cut(s) 938
MhlI GDGCHC 2 cut(s) 278, 472
MlsI TGGCCA 2 cut(s) 5, 519
MluNI TGGCCA 2 cut(s) 5, 519
MlyI GAGTC 4 cut(s) 514, 876, 970, 1333
MmeI TCCRAC 3 cut(s) 139, 482, 970
MnlI CCTC 9 cut(s) 122, 204, 208, 209, 402, 414, 506, 820, 1175
Mox20I TGGCCA 2 cut(s) 5, 519
MroNI GCCGGC 1 cut(s) 183
MroXI GAANNNNTTC 2 cut(s) 917, 968
MscI TGGCCA 2 cut(s) 5, 519
MseI TTAA 4 cut(s) 44, 90, 951, 1109
Msp20I TGGCCA 2 cut(s) 5, 519
MspA1I CMGCKG 2 cut(s) 587, 936
MspI CCGG 7 cut(s) 184, 224, 308, 429, 647, 716, 1130
MspR9I CCNGG 5 cut(s) 224, 415, 716, 1344, 1386
MunI CAATTG 1 cut(s) 1371
Mva1269I GAATGC 3 cut(s) 303, 430, 1105
MvaI CCWGG 3 cut(s) 415, 1344, 1386
MvnI CGCG 2 cut(s) 770, 847
MwoI GCNNNNNNNGC 6 cut(s) 248, 494, 584, 1076, 1100, 1226
NaeI GCCGGC 1 cut(s) 185
NciI CCSGG 2 cut(s) 224, 716
NcoI CCATGG 1 cut(s) 76
NdeII GATC 5 cut(s) 711, 811, 938, 1136, 1188
NgoMIV GCCGGC 1 cut(s) 183
NheI GCTAGC 1 cut(s) 452
NlaIII CATG 3 cut(s) 80, 302, 558
NlaIV GGNNCC 8 cut(s) 58, 276, 277, 382, 390, 433, 745, 1521
NmuCI GTSAC 2 cut(s) 409, 1336
PaqCI CACCTGC 1 cut(s) 502
PctI GAATGC 3 cut(s) 303, 430, 1105
PdiI GCCGGC 1 cut(s) 185
PdmI GAANNNNTTC 2 cut(s) 917, 968
PfeI GAWTC 6 cut(s) 31, 903, 913, 1328, 1380, 1389
PflFI GACNNNGTC 1 cut(s) 760
PflMI CCANNNNNTGG 1 cut(s) 686
PkrI GCNGC 7 cut(s) 375, 490, 589, 592, 935, 1222, 1261
PleI GAGTC 4 cut(s) 513, 876, 970, 1333
PpsI GAGTC 4 cut(s) 513, 876, 970, 1333
Psp6I CCWGG 3 cut(s) 413, 1342, 1384
PspEI GGTNACC 1 cut(s) 409
PspFI CCCAGC 2 cut(s) 704, 1403
PspGI CCWGG 3 cut(s) 413, 1342, 1384
PspN4I GGNNCC 8 cut(s) 58, 276, 277, 382, 390, 433, 745, 1521
PspOMI GGGCCC 1 cut(s) 274
PspPI GGNCC 4 cut(s) 274, 275, 572, 743
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 938
PsyI GACNNNGTC 1 cut(s) 760
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 2 cut(s) 839, 1425
RsaNI GTAC 2 cut(s) 838, 1424
SaqAI TTAA 4 cut(s) 44, 90, 951, 1109
SatI GCNGC 7 cut(s) 374, 489, 588, 591, 934, 1221, 1260
Sau3AI GATC 5 cut(s) 711, 811, 938, 1136, 1188
Sau96I GGNCC 4 cut(s) 274, 275, 572, 743
SchI GAGTC 4 cut(s) 514, 876, 970, 1333
ScrFI CCNGG 5 cut(s) 224, 415, 716, 1344, 1386
SduI GDGCHC 2 cut(s) 278, 472
SexAI ACCWGGT 1 cut(s) 413
SfaNI GCATC 2 cut(s) 388, 681
SfcI CTRYAG 1 cut(s) 663
SinI GGWCC 2 cut(s) 572, 743
SmlI CTYRAG 4 cut(s) 317, 457, 562, 1166
SmoI CTYRAG 4 cut(s) 317, 457, 562, 1166
SsiI CCGC 5 cut(s) 181, 590, 770, 845, 936
SspMI CTAG 4 cut(s) 453, 1013, 1091, 1118
StyD4I CCNGG 5 cut(s) 222, 413, 714, 1342, 1384
StyI CCWWGG 2 cut(s) 76, 520
TaaI ACNGT 2 cut(s) 685, 760
TaiI ACGT 1 cut(s) 231
TaqI TCGA 1 cut(s) 1135
TatI WGTACW 1 cut(s) 837
TauI GCSGC 1 cut(s) 593
TfiI GAWTC 6 cut(s) 31, 903, 913, 1328, 1380, 1389
Tru1I TTAA 4 cut(s) 44, 90, 951, 1109
Tru9I TTAA 4 cut(s) 44, 90, 951, 1109
TscAI CASTG 4 cut(s) 574, 765, 1080, 1399
TseFI GTSAC 2 cut(s) 409, 1336
TseI GCWGC 6 cut(s) 373, 488, 587, 933, 1220, 1259
Tsp45I GTSAC 2 cut(s) 409, 1336
TspDTI ATGAA 3 cut(s) 39, 1266, 1320
TspGWI ACGGA 3 cut(s) 6, 373, 906
TspRI CASTG 4 cut(s) 574, 765, 1080, 1399
Tth111I GACNNNGTC 1 cut(s) 760
Van91I CCANNNNNTGG 1 cut(s) 686
VpaK11BI GGWCC 2 cut(s) 572, 743
XapI RAATTY 3 cut(s) 595, 1196, 1270
XbaI TCTAGA 1 cut(s) 1012
XcmI CCANNNNNNNNNTGG 2 cut(s) 1072, 1232
XmiI GTMKAC 1 cut(s) 1005
XmnI GAANNNNTTC 2 cut(s) 917, 968
XspI CTAG 4 cut(s) 453, 1013, 1091, 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.