Rmu_sc0002522.1_g000022

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002522.1
OG0000427
Physical Location & Seq
Reverse (-)
76964 .. 79649
2686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002522.1_g000022.1.cds

Sequence Viewer

Length: 1335 bp
atggaagaagccacagttgatgatctccaactcgctttcaagcaaaagcttctgacttcaaggcaagtcgttcagttctacctagaccaaatccacaaactcaaccaagacctcagaggagtcttagagataaacccagatgctttgtcgcaagctgacatagcagacaaagagcgcagtgaatatgaatccagtgcccaaacactgcctaagcttcatgggattcctgttctgctcaaggataatatagcaaccaaggacaagcttaacaccactgctgggtcttatgctcttttgggttccgtagtgcctagagatgctggggttgtctcaaagttgaggagctctggggctatcattttggggaaggctccgttgagtgaatgggcctatatcagaacatctaaagcaccctatggttggagtgccagaggtggtcaaggagtgaatccttataattcatcactggaagtttgtggatcaagtagtggatccgcaatatcagtatctgcaaatatggtatcagtgtctctaggaacagagacctatagctccatcttatgtccagcgagttttaactcggtagtgggcttcaaaccaacggttggtctcactagtcgagcaggagtcatcccagtcagtccaagacaggacacaattgggccaatttgtaggactgtagcagatgctgttcacgtccttgataccattgttggcattgatagcaatgacaaggcaacaaatgaagtatctcagtatattccagtgggtggctatggccagtttctcaattctaatggtctcaaaggaaagagactggggatagtcagtctcatgtttcttcagatcaaggatgatgctttcttgaatcaaacttttgagcagcattttaccacactgagggaactaggtgcaattttggttgagaatctggaaatagccaatcttgaagatattcacgattttacttcaagtggtgagtttgtagcaatgttagctgaattcaagatcgccttgaatgcgtacctgggtgacctagtcaaatctccagtgagaaccttagcccaggttgtagcattcaacaagaataattcttatctggaaaagaatgatgattatggacaaaatctcttacaagcagctgaggagactaatggaatgggagcaaaagagcttgaggcattgtcaaatatagagagactatctagagatggatttgagaagttgatgacaaataataacttagatgcattggtgacttatgctaatagcgcttcttctatcctcgcaattggtggtttcccaggcatcgttgtgccagcaggatatcattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

444

Amino Acids

47.72

Weight (kDa)

5.15

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 456
AccB7I CCANNNNNTGG 2 cut(s) 605, 770
AciI CCGC 1 cut(s) 495
AclWI GGATC 3 cut(s) 486, 487, 499
AcoI YGGCCR 1 cut(s) 778
AcsI RAATTY 1 cut(s) 1001
AcuI CTGAAG 1 cut(s) 827
AfaI GTAC 1 cut(s) 1025
AfeI AGCGCT 1 cut(s) 1273
AfiI CCNNNNNNNGG 5 cut(s) 279, 420, 605, 770, 900
AgsI TTSAA 9 cut(s) 40, 60, 595, 868, 950, 972, 1006, 1018, 1081
AhlI ACTAGT 1 cut(s) 614
AjiI CACGTC 1 cut(s) 697
AjnI CCWGG 3 cut(s) 1026, 1065, 1303
AluBI AGCT 9 cut(s) 49, 155, 214, 265, 345, 552, 998, 1142, 1174
AluI AGCT 9 cut(s) 49, 155, 214, 265, 345, 552, 998, 1142, 1174
Alw21I GWGCWC 1 cut(s) 347
Alw26I GTCTC 9 cut(s) 334, 534, 536, 614, 806, 808, 836, 1142, 1192
AlwI GGATC 3 cut(s) 486, 487, 499
AlwNI CAGNNNCTG 2 cut(s) 509, 689
Aor51HI AGCGCT 1 cut(s) 1273
AoxI GGCC 3 cut(s) 387, 662, 778
ApeKI GCWGC 2 cut(s) 883, 1139
ApoI RAATTY 1 cut(s) 1001
Asp700I GAANNNNTTC 1 cut(s) 954
AspLEI GCGC 2 cut(s) 177, 1274
AspS9I GGNCC 2 cut(s) 387, 662
AsuHPI GGTGA 3 cut(s) 989, 1043, 1267
BaeGI GKGCMC 1 cut(s) 199
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BalI TGGCCA 1 cut(s) 780
BamHI GGATCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 347
BbvCI CCTCAGC 1 cut(s) 1143
BbvI GCAGC 2 cut(s) 895, 1151
BccI CCATC 2 cut(s) 563, 1205
BciT130I CCWGG 3 cut(s) 1028, 1067, 1305
BcoDI GTCTC 9 cut(s) 334, 534, 536, 614, 806, 808, 836, 1142, 1192
BcuI ACTAGT 1 cut(s) 614
BfaI CTAG 7 cut(s) 83, 312, 533, 615, 908, 1037, 1206
BfmI CTRYAG 2 cut(s) 547, 678
BfoI RGCGCY 1 cut(s) 1275
BisI GCNGC 2 cut(s) 884, 1140
BlsI GCNGC 2 cut(s) 885, 1141
Bme1390I CCNGG 3 cut(s) 1028, 1067, 1305
BmgBI CACGTC 1 cut(s) 697
BmgT120I GGNCC 2 cut(s) 387, 662
BmiI GGNNCC 3 cut(s) 301, 372, 493
BmrFI CCNGG 3 cut(s) 1028, 1067, 1305
BmrI ACTGGG 2 cut(s) 629, 827
BmsI GCATC 6 cut(s) 130, 307, 676, 847, 1237, 1317
BmuI ACTGGG 2 cut(s) 629, 827
BpmI CTGGAG 1 cut(s) 1032
Bpu10I CCTNAGC 3 cut(s) 210, 1060, 1143
BpuEI CTTGAG 2 cut(s) 221, 1196
BsaI GGTCTC 3 cut(s) 536, 614, 806
BsaJI CCNNGG 4 cut(s) 255, 1027, 1065, 1303
Bsc4I CCNNNNNNNGG 5 cut(s) 279, 420, 605, 770, 900
Bse1I ACTGG 7 cut(s) 192, 471, 635, 764, 781, 822, 1049
Bse3DI GCAATG 2 cut(s) 733, 996
BseBI CCWGG 3 cut(s) 1028, 1067, 1305
BseDI CCNNGG 4 cut(s) 255, 1027, 1065, 1303
BseGI GGATG 2 cut(s) 630, 859
BseLI CCNNNNNNNGG 5 cut(s) 279, 420, 605, 770, 900
BseMI GCAATG 2 cut(s) 733, 996
BseMII CTCAG 4 cut(s) 127, 767, 890, 1134
BseNI ACTGG 7 cut(s) 192, 471, 635, 764, 781, 822, 1049
BseRI GAGGAG 3 cut(s) 132, 355, 1160
BseSI GKGCMC 1 cut(s) 199
BseXI GCAGC 2 cut(s) 895, 1151
BseYI CCCAGC 2 cut(s) 278, 320
BshFI GGCC 3 cut(s) 389, 664, 780
BsiHKAI GWGCWC 1 cut(s) 347
BslI CCNNNNNNNGG 5 cut(s) 279, 420, 605, 770, 900
BsmAI GTCTC 9 cut(s) 334, 534, 536, 614, 806, 808, 836, 1142, 1192
BsmI GAATGC 2 cut(s) 1024, 1076
BsnI GGCC 3 cut(s) 389, 664, 780
Bso31I GGTCTC 3 cut(s) 536, 614, 806
Bsp1286I GDGCHC 2 cut(s) 199, 347
Bsp143I GATC 5 cut(s) 22, 479, 491, 846, 1008
BspACI CCGC 1 cut(s) 495
BspANI GGCC 3 cut(s) 389, 664, 780
BspCNI CTCAG 4 cut(s) 126, 766, 891, 1135
BspLI GGNNCC 3 cut(s) 301, 372, 493
BspPI GGATC 3 cut(s) 486, 487, 499
BspTNI GGTCTC 3 cut(s) 536, 614, 806
BsrDI GCAATG 2 cut(s) 733, 996
BsrI ACTGG 7 cut(s) 192, 471, 635, 764, 781, 822, 1049
BssECI CCNNGG 4 cut(s) 255, 1027, 1065, 1303
BssMI GATC 5 cut(s) 22, 479, 491, 846, 1008
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 3 cut(s) 1028, 1067, 1305
Bst4CI ACNGT 3 cut(s) 16, 604, 679
BstC8I GCNNGC 2 cut(s) 153, 1320
BstDEI CTNAG 8 cut(s) 113, 124, 210, 753, 899, 1060, 1143, 1243
BstEII GGTNACC 1 cut(s) 1031
BstF5I GGATG 2 cut(s) 630, 859
BstH2I RGCGCY 1 cut(s) 1275
BstHHI GCGC 2 cut(s) 177, 1274
BstKTI GATC 5 cut(s) 25, 482, 494, 849, 1011
BstMAI GTCTC 9 cut(s) 334, 534, 536, 614, 806, 808, 836, 1142, 1192
BstMBI GATC 5 cut(s) 22, 479, 491, 846, 1008
BstMWI GCNNNNNNNGC 5 cut(s) 161, 723, 995, 1019, 1271
BstNI CCWGG 3 cut(s) 1028, 1067, 1305
BstPI GGTNACC 1 cut(s) 1031
BstSCI CCNGG 3 cut(s) 1026, 1065, 1303
BstSFI CTRYAG 2 cut(s) 547, 678
BstSLI GKGCMC 1 cut(s) 199
BstV1I GCAGC 2 cut(s) 895, 1151
BstX2I RGATCY 1 cut(s) 491
BstYI RGATCY 1 cut(s) 491
BsuRI GGCC 3 cut(s) 389, 664, 780
BtrI CACGTC 1 cut(s) 697
BtsCI GGATG 2 cut(s) 630, 859
BtsI GCAGTG 3 cut(s) 184, 203, 273
BtsIMutI CAGTG 9 cut(s) 184, 199, 203, 273, 464, 531, 771, 896, 1056
Cac8I GCNNGC 2 cut(s) 153, 1320
CaiI CAGNNNCTG 2 cut(s) 509, 689
CfoI GCGC 2 cut(s) 177, 1274
Cfr13I GGNCC 2 cut(s) 387, 662
Csp6I GTAC 1 cut(s) 1024
CviAII CATG 2 cut(s) 218, 835
CviQI GTAC 1 cut(s) 1024
DdeI CTNAG 8 cut(s) 113, 124, 210, 753, 899, 1060, 1143, 1243
DpnI GATC 5 cut(s) 24, 481, 493, 848, 1010
DpnII GATC 5 cut(s) 22, 479, 491, 846, 1008
EaeI YGGCCR 1 cut(s) 778
Ecl136II GAGCTC 1 cut(s) 345
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 347
Eco31I GGTCTC 3 cut(s) 536, 614, 806
Eco32I GATATC 1 cut(s) 1328
Eco47III AGCGCT 1 cut(s) 1273
Eco53kI GAGCTC 1 cut(s) 345
Eco57I CTGAAG 1 cut(s) 827
Eco91I GGTNACC 1 cut(s) 1031
EcoICRI GAGCTC 1 cut(s) 345
EcoO65I GGTNACC 1 cut(s) 1031
EcoRI GAATTC 1 cut(s) 1001
EcoRII CCWGG 3 cut(s) 1026, 1065, 1303
EcoRV GATATC 1 cut(s) 1328
EcoT14I CCWWGG 1 cut(s) 255
EcoT22I ATGCAT 1 cut(s) 1252
EcoT38I GRGCYC 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 2 cut(s) 221, 838
FalI AAGNNNNNCTT 2 cut(s) 998, 1030
FatI CATG 2 cut(s) 217, 834
Fnu4HI GCNGC 2 cut(s) 884, 1140
FokI GGATG 2 cut(s) 617, 866
FriOI GRGCYC 1 cut(s) 347
Fsp4HI GCNGC 2 cut(s) 884, 1140
FspBI CTAG 7 cut(s) 83, 312, 533, 615, 908, 1037, 1206
GlaI GCGC 2 cut(s) 176, 1273
GluI GCNGC 2 cut(s) 884, 1140
GsaI CCCAGC 2 cut(s) 282, 324
GsuI CTGGAG 1 cut(s) 1032
HaeII RGCGCY 1 cut(s) 1275
HaeIII GGCC 3 cut(s) 389, 664, 780
HhaI GCGC 2 cut(s) 177, 1274
Hin1II CATG 2 cut(s) 221, 838
Hin6I GCGC 2 cut(s) 175, 1272
HinP1I GCGC 2 cut(s) 175, 1272
HindIII AAGCTT 3 cut(s) 47, 212, 263
HinfI GANTC 7 cut(s) 120, 188, 223, 448, 627, 868, 928
HphI GGTGA 3 cut(s) 989, 1043, 1267
Hpy166II GTNNAC 1 cut(s) 694
Hpy188I TCNGA 4 cut(s) 54, 116, 398, 846
Hpy188III TCNNGA 7 cut(s) 865, 932, 947, 959, 1006, 1100, 1206
Hpy8I GTNNAC 1 cut(s) 694
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 3 cut(s) 16, 604, 679
HpyCH4IV ACGT 1 cut(s) 696
HpyCH4V TGCA 3 cut(s) 512, 914, 1250
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 723, 995, 1019, 1271
HpyF3I CTNAG 8 cut(s) 113, 124, 210, 753, 899, 1060, 1143, 1243
HpySE526I ACGT 1 cut(s) 696
Hsp92II CATG 2 cut(s) 221, 838
HspAI GCGC 2 cut(s) 175, 1272
Kzo9I GATC 5 cut(s) 22, 479, 491, 846, 1008
LmnI GCTCC 4 cut(s) 342, 376, 557, 1163
Lsp1109I GCAGC 2 cut(s) 895, 1151
LweI GCATC 6 cut(s) 130, 307, 676, 847, 1237, 1317
MaeI CTAG 7 cut(s) 83, 312, 533, 615, 908, 1037, 1206
MaeII ACGT 1 cut(s) 696
MaeIII GTNAC 2 cut(s) 1031, 1255
MalI GATC 5 cut(s) 24, 481, 493, 848, 1010
MboI GATC 5 cut(s) 22, 479, 491, 846, 1008
MboII GAAGA 4 cut(s) 17, 833, 962, 1269
MfeI CAATTG 2 cut(s) 657, 1290
MflI RGATCY 1 cut(s) 491
MhlI GDGCHC 2 cut(s) 199, 347
MlsI TGGCCA 1 cut(s) 780
MluCI AATT 8 cut(s) 457, 657, 666, 790, 915, 1001, 1090, 1290
MluNI TGGCCA 1 cut(s) 780
MlyI GAGTC 2 cut(s) 129, 636
MmeI TCCRAC 2 cut(s) 52, 401
MnlI CCTC 8 cut(s) 110, 122, 333, 425, 894, 1138, 1171, 1295
Mox20I TGGCCA 1 cut(s) 780
Mph1103I ATGCAT 1 cut(s) 1252
MroXI GAANNNNTTC 1 cut(s) 954
MscI TGGCCA 1 cut(s) 780
MseI TTAA 2 cut(s) 267, 576
MslI CAYNNNNRTG 1 cut(s) 1313
Msp20I TGGCCA 1 cut(s) 780
MspA1I CMGCKG 1 cut(s) 1142
MspR9I CCNGG 3 cut(s) 1028, 1067, 1305
MunI CAATTG 2 cut(s) 657, 1290
Mva1269I GAATGC 2 cut(s) 1024, 1076
MvaI CCWGG 3 cut(s) 1028, 1067, 1305
MwoI GCNNNNNNNGC 5 cut(s) 161, 723, 995, 1019, 1271
NdeII GATC 5 cut(s) 22, 479, 491, 846, 1008
NlaIII CATG 2 cut(s) 221, 838
NlaIV GGNNCC 3 cut(s) 301, 372, 493
NmuCI GTSAC 2 cut(s) 1031, 1255
NsiI ATGCAT 1 cut(s) 1252
PctI GAATGC 2 cut(s) 1024, 1076
PdmI GAANNNNTTC 1 cut(s) 954
PfeI GAWTC 5 cut(s) 188, 223, 448, 868, 928
PflFI GACNNNGTC 1 cut(s) 1037
PflMI CCANNNNNTGG 2 cut(s) 605, 770
PkrI GCNGC 2 cut(s) 885, 1141
PleI GAGTC 2 cut(s) 128, 635
PpsI GAGTC 2 cut(s) 128, 635
PsiI TTATAA 1 cut(s) 456
Psp124BI GAGCTC 1 cut(s) 347
Psp6I CCWGG 3 cut(s) 1026, 1065, 1303
PspEI GGTNACC 1 cut(s) 1031
PspFI CCCAGC 2 cut(s) 278, 320
PspGI CCWGG 3 cut(s) 1026, 1065, 1303
PspN4I GGNNCC 3 cut(s) 301, 372, 493
PspPI GGNCC 2 cut(s) 387, 662
PstNI CAGNNNCTG 2 cut(s) 509, 689
PsuI RGATCY 1 cut(s) 491
PsyI GACNNNGTC 1 cut(s) 1037
PvuII CAGCTG 1 cut(s) 1142
RsaI GTAC 1 cut(s) 1025
RsaNI GTAC 1 cut(s) 1024
RseI CAYNNNNRTG 1 cut(s) 1313
SacI GAGCTC 1 cut(s) 347
SaqAI TTAA 2 cut(s) 267, 576
SatI GCNGC 2 cut(s) 884, 1140
Sau3AI GATC 5 cut(s) 22, 479, 491, 846, 1008
Sau96I GGNCC 2 cut(s) 387, 662
SchI GAGTC 2 cut(s) 129, 636
ScrFI CCNGG 3 cut(s) 1028, 1067, 1305
SduI GDGCHC 2 cut(s) 199, 347
SfaNI GCATC 6 cut(s) 130, 307, 676, 847, 1237, 1317
SfcI CTRYAG 2 cut(s) 547, 678
SmiMI CAYNNNNRTG 1 cut(s) 1313
SmlI CTYRAG 2 cut(s) 236, 1175
SmoI CTYRAG 2 cut(s) 236, 1175
SpeI ACTAGT 1 cut(s) 614
Sse9I AATT 8 cut(s) 457, 657, 666, 790, 915, 1001, 1090, 1290
SsiI CCGC 1 cut(s) 495
SspMI CTAG 7 cut(s) 83, 312, 533, 615, 908, 1037, 1206
SstI GAGCTC 1 cut(s) 347
StyD4I CCNGG 3 cut(s) 1026, 1065, 1303
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 3 cut(s) 16, 604, 679
TaiI ACGT 1 cut(s) 699
TaqI TCGA 1 cut(s) 619
TasI AATT 8 cut(s) 457, 657, 666, 790, 915, 1001, 1090, 1290
TfiI GAWTC 5 cut(s) 188, 223, 448, 868, 928
Tru1I TTAA 2 cut(s) 267, 576
Tru9I TTAA 2 cut(s) 267, 576
TscAI CASTG 9 cut(s) 184, 199, 210, 280, 471, 531, 771, 903, 1056
TseFI GTSAC 2 cut(s) 1031, 1255
TseI GCWGC 2 cut(s) 883, 1139
Tsp45I GTSAC 2 cut(s) 1031, 1255
TspDTI ATGAA 4 cut(s) 201, 206, 450, 759
TspGWI ACGGA 2 cut(s) 292, 363
TspRI CASTG 9 cut(s) 184, 199, 210, 280, 471, 531, 771, 903, 1056
Tth111I GACNNNGTC 1 cut(s) 1037
Van91I CCANNNNNTGG 2 cut(s) 605, 770
XapI RAATTY 1 cut(s) 1001
XbaI TCTAGA 1 cut(s) 1205
XmnI GAANNNNTTC 1 cut(s) 954
XspI CTAG 7 cut(s) 83, 312, 533, 615, 908, 1037, 1206
Zsp2I ATGCAT 1 cut(s) 1252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.