RLG00000015333

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000427
Physical Location & Seq
Forward (+)
64703127 .. 64705326
2200 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015333

Sequence Viewer

Length: 1554 bp
ATGACGAAACCAAGCTTCCCTCACATCTTATCTTTATTGCTTCTCATTCTTCTCTTCACATTATCATCTCCGCCAGGCACATTCGCAGCCCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTTAAGCACAAGCAACTCACCTCGAGGCAACTTGTCCAATATTACCTTTCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAACCCGGATGCGCTTCAACTTGCCGACAAGGCTGACGATGAGCGCAGGACCAAGGCATCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACTACCGCCGGCTCTTTTGCGCTTCTTGGATCTGTCGTCCCTCGTGATGCCGGAGTGGTGGAGAAGTTGAGGAAAGCAGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCATATTTTAGGTCTAATACAGCACCAAATGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACCCTTACACGTTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTAGCATCAAATCTGGCAGCGGTGTCATTAGGTACTGAGACAGACGGTTCAATCTTATGTCCATCAGGTTACAACTCGGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGAGTTGTCCCAATTTCTCCAAGACAGGATACTGTTGGGCCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATAGAGACCTCAAAGTACATTCCCAGAGGTGGTTATGCACAGTTTCTCAGGCGCGAGGGACTTGGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTGGAAATTGCTAATTTGACTGAAATCTATTCTTCAAGCGATGAAGATACTGCATTGTCTGTTGAGTTCAAAATATCCTTAAATGCTTACTTGAAGGAGTTGGTGGCTTCCCCAGTGCAATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCCGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACGTATTTTTAGCGGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGCAGCCGGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGCTGTGGTGACACCTAACGCAGGTGTTTCAAGAGTACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCCGGATACGAAAACAATGGGAGACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCTACTAAGATTAGGAAGCCTCCTTCATTCAAAGGCTTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

518

Amino Acids

55.42

Weight (kDa)

9.35

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 57 - 452 1.3e-69 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1346
AbsI CCTCGAGG 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 1346
AccI GTMKAC 1 cut(s) 1017
AccII CGCG 1 cut(s) 891
AciI CCGC 8 cut(s) 71, 91, 375, 534, 573, 585, 620, 1226
AclWI GGATC 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 1251
AfaI GTAC 3 cut(s) 634, 854, 1371
AfiI CCNNNNNNNGG 4 cut(s) 701, 866, 1186, 1355
AflIII ACRYGT 1 cut(s) 555
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 2 cut(s) 73, 1390
AluBI AGCT 7 cut(s) 15, 133, 474, 1330, 1436, 1477, 1508
AluI AGCT 7 cut(s) 15, 133, 474, 1330, 1436, 1477, 1508
Alw26I GTCTC 3 cut(s) 632, 836, 1423
AlwI GGATC 1 cut(s) 406
AlwNI CAGNNNCTG 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 1 cut(s) 758
ApeKI GCWGC 5 cut(s) 86, 582, 617, 785, 1261
AspLEI GCGC 5 cut(s) 247, 279, 391, 527, 891
AspS9I GGNCC 2 cut(s) 282, 758
AsuC2I CCSGG 2 cut(s) 209, 239
AsuHPI GGTGA 3 cut(s) 148, 1327, 1354
AsuNHI GCTAGC 1 cut(s) 470
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 1390
BauI CACGAG 1 cut(s) 411
BbvI GCAGC 5 cut(s) 98, 594, 629, 772, 1273
BccI CCATC 1 cut(s) 670
BciT130I CCWGG 2 cut(s) 75, 1392
BciVI GTATCC 2 cut(s) 742, 1406
BcnI CCSGG 2 cut(s) 209, 239
BcoDI GTCTC 3 cut(s) 632, 836, 1423
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 4 cut(s) 227, 471, 711, 1331
BfoI RGCGCY 1 cut(s) 528
BfuAI ACCTGC 1 cut(s) 1346
BfuI GTATCC 2 cut(s) 742, 1406
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 7 cut(s) 87, 574, 583, 618, 786, 1227, 1262
BlsI GCNGC 7 cut(s) 88, 575, 584, 619, 787, 1228, 1263
BmcAI AGTACT 1 cut(s) 1371
Bme1390I CCNGG 4 cut(s) 75, 209, 239, 1392
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 282, 758
BmiI GGNNCC 1 cut(s) 1387
BmrFI CCNGG 4 cut(s) 75, 209, 239, 1392
BmrI ACTGGG 1 cut(s) 1130
BmsI GCATC 9 cut(s) 232, 299, 406, 614, 696, 772, 790, 822, 1324
BmtI GCTAGC 1 cut(s) 474
BmuI ACTGGG 1 cut(s) 1130
BpmI CTGGAG 1 cut(s) 113
BpuEI CTTGAG 2 cut(s) 496, 562
BpuMI CCSGG 2 cut(s) 209, 239
BsaI GGTCTC 2 cut(s) 836, 1423
BsaJI CCNNGG 5 cut(s) 285, 354, 957, 1146, 1390
BsaXI ACNNNNNCTCC 2 cut(s) 993, 1023
Bsc4I CCNNNNNNNGG 4 cut(s) 701, 866, 1186, 1355
Bse118I RCCGGY 2 cut(s) 377, 1264
Bse1I ACTGG 2 cut(s) 1136, 1191
Bse3DI GCAATG 3 cut(s) 345, 828, 952
BseBI CCWGG 2 cut(s) 75, 1392
BseDI CCNNGG 5 cut(s) 285, 354, 957, 1146, 1390
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 4 cut(s) 701, 866, 1186, 1355
BseMI GCAATG 3 cut(s) 345, 828, 952
BseMII CTCAG 4 cut(s) 576, 627, 898, 983
BseNI ACTGG 2 cut(s) 1136, 1191
BseXI GCAGC 5 cut(s) 98, 594, 629, 772, 1273
Bsh1236I CGCG 1 cut(s) 891
BshFI GGCC 1 cut(s) 760
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 6 cut(s) 209, 239, 378, 420, 1265, 1410
BslFI GGGAC 3 cut(s) 392, 713, 909
BslI CCNNNNNNNGG 4 cut(s) 701, 866, 1186, 1355
BsmAI GTCTC 3 cut(s) 632, 836, 1423
BsmFI GGGAC 3 cut(s) 392, 713, 909
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 1 cut(s) 760
Bso31I GGTCTC 2 cut(s) 836, 1423
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 1390
Bsp143I GATC 3 cut(s) 124, 398, 1194
BspACI CCGC 8 cut(s) 71, 91, 375, 534, 573, 585, 620, 1226
BspANI GGCC 1 cut(s) 760
BspCNI CTCAG 4 cut(s) 575, 628, 897, 984
BspFNI CGCG 1 cut(s) 891
BspLI GGNNCC 1 cut(s) 1387
BspMI ACCTGC 1 cut(s) 1346
BspOI GCTAGC 1 cut(s) 474
BspPI GGATC 1 cut(s) 406
BspTNI GGTCTC 2 cut(s) 836, 1423
BsrDI GCAATG 3 cut(s) 345, 828, 952
BsrFI RCCGGY 2 cut(s) 377, 1264
BsrI ACTGG 2 cut(s) 1136, 1191
BssAI RCCGGY 2 cut(s) 377, 1264
BssECI CCNNGG 5 cut(s) 285, 354, 957, 1146, 1390
BssMI GATC 3 cut(s) 124, 398, 1194
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 4 cut(s) 285, 354, 957, 1146
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 2 cut(s) 75, 1392
Bst4CI ACNGT 4 cut(s) 647, 754, 775, 879
Bst6I CTCTTC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 785
BstC8I GCNNGC 7 cut(s) 91, 135, 379, 472, 811, 1266, 1506
BstDEI CTNAG 6 cut(s) 309, 562, 636, 884, 992, 1512
BstENI CCTNNNNNAGG 1 cut(s) 1353
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 1 cut(s) 891
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 5 cut(s) 247, 279, 391, 527, 891
BstKTI GATC 3 cut(s) 127, 401, 1197
BstMAI GTCTC 3 cut(s) 632, 836, 1423
BstMBI GATC 3 cut(s) 124, 398, 1194
BstMWI GCNNNNNNNGC 7 cut(s) 263, 531, 540, 579, 582, 785, 1232
BstNI CCWGG 2 cut(s) 75, 1392
BstSCI CCNGG 4 cut(s) 73, 207, 237, 1390
BstUI CGCG 1 cut(s) 891
BstV1I GCAGC 5 cut(s) 98, 594, 629, 772, 1273
BstX2I RGATCY 1 cut(s) 398
BstXI CCANNNNNNTGG 1 cut(s) 519
BstYI RGATCY 1 cut(s) 398
BsuI GTATCC 2 cut(s) 742, 1406
BsuRI GGCC 1 cut(s) 760
BtgZI GCGATG 1 cut(s) 1077
BtsCI GGATG 1 cut(s) 247
BtsIMutI CAGTG 2 cut(s) 300, 1143
BveI ACCTGC 1 cut(s) 1346
Cac8I GCNNGC 7 cut(s) 91, 135, 379, 472, 811, 1266, 1506
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 5 cut(s) 247, 279, 391, 527, 891
Cfr10I RCCGGY 2 cut(s) 377, 1264
Cfr13I GGNCC 2 cut(s) 282, 758
Csp6I GTAC 3 cut(s) 633, 853, 1370
CviAII CATG 1 cut(s) 317
CviQI GTAC 3 cut(s) 633, 853, 1370
DdeI CTNAG 6 cut(s) 309, 562, 636, 884, 992, 1512
DpnI GATC 3 cut(s) 126, 400, 1196
DpnII GATC 3 cut(s) 124, 398, 1194
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
EciI GGCGGA 1 cut(s) 60
Eco130I CCWWGG 4 cut(s) 285, 354, 957, 1146
Eco24I GRGCYC 1 cut(s) 1390
Eco31I GGTCTC 2 cut(s) 836, 1423
Eco47I GGWCC 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 1251
Eco88I CYCGRG 1 cut(s) 160
EcoNI CCTNNNNNAGG 1 cut(s) 1353
EcoRII CCWGG 2 cut(s) 73, 1390
EcoT14I CCWWGG 4 cut(s) 285, 354, 957, 1146
EcoT38I GRGCYC 1 cut(s) 1390
ErhI CCWWGG 4 cut(s) 285, 354, 957, 1146
FaeI CATG 1 cut(s) 320
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 3 cut(s) 392, 713, 909
FatI CATG 1 cut(s) 316
FauI CCCGC 1 cut(s) 98
FauNDI CATATG 1 cut(s) 1492
FblI GTMKAC 1 cut(s) 1017
Fnu4HI GCNGC 7 cut(s) 87, 574, 583, 618, 786, 1227, 1262
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 1390
Fsp4HI GCNGC 7 cut(s) 87, 574, 583, 618, 786, 1227, 1262
FspBI CTAG 4 cut(s) 227, 471, 711, 1331
GlaI GCGC 5 cut(s) 246, 278, 390, 526, 890
GluI GCNGC 7 cut(s) 87, 574, 583, 618, 786, 1227, 1262
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 1 cut(s) 760
HapII CCGG 6 cut(s) 209, 239, 378, 420, 1265, 1410
HhaI GCGC 5 cut(s) 247, 279, 391, 527, 891
Hin1II CATG 1 cut(s) 320
Hin6I GCGC 5 cut(s) 245, 277, 389, 525, 889
HinP1I GCGC 5 cut(s) 245, 277, 389, 525, 889
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 3 cut(s) 96, 222, 1276
HpaII CCGG 6 cut(s) 209, 239, 378, 420, 1265, 1410
HphI GGTGA 3 cut(s) 148, 1327, 1354
Hpy166II GTNNAC 4 cut(s) 235, 1018, 1290, 1454
Hpy188I TCNGA 4 cut(s) 197, 565, 993, 1468
Hpy188III TCNNGA 4 cut(s) 413, 797, 1365, 1483
Hpy8I GTNNAC 4 cut(s) 235, 1018, 1290, 1454
HpyAV CCTTC 4 cut(s) 940, 1111, 1174, 1539
HpyCH4III ACNGT 4 cut(s) 647, 754, 775, 879
HpyCH4IV ACGT 2 cut(s) 557, 1215
HpyCH4V TGCA 9 cut(s) 316, 350, 833, 875, 1076, 1141, 1283, 1376, 1490
HpyF10VI GCNNNNNNNGC 7 cut(s) 263, 531, 540, 579, 582, 785, 1232
HpyF3I CTNAG 6 cut(s) 309, 562, 636, 884, 992, 1512
HpySE526I ACGT 2 cut(s) 557, 1215
Hsp92II CATG 1 cut(s) 320
HspAI GCGC 5 cut(s) 245, 277, 389, 525, 889
KroI GCCGGC 2 cut(s) 377, 1264
KroNI GCCGGC 2 cut(s) 379, 1266
Kzo9I GATC 3 cut(s) 124, 398, 1194
LmnI GCTCC 2 cut(s) 522, 1385
Lsp1109I GCAGC 5 cut(s) 98, 594, 629, 772, 1273
LweI GCATC 9 cut(s) 232, 299, 406, 614, 696, 772, 790, 822, 1324
MaeI CTAG 4 cut(s) 227, 471, 711, 1331
MaeII ACGT 2 cut(s) 557, 1215
MaeIII GTNAC 4 cut(s) 297, 668, 1315, 1342
MalI GATC 3 cut(s) 126, 400, 1196
MboI GATC 3 cut(s) 124, 398, 1194
MboII GAAGA 5 cut(s) 41, 46, 1047, 1079, 1336
MfeI CAATTG 1 cut(s) 1377
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 1390
MluCI AATT 7 cut(s) 732, 762, 1021, 1029, 1036, 1310, 1377
MlyI GAGTC 2 cut(s) 90, 231
MmeI TCCRAC 1 cut(s) 500
MroNI GCCGGC 2 cut(s) 377, 1264
MseI TTAA 2 cut(s) 141, 1103
MspA1I CMGCKG 3 cut(s) 585, 620, 1436
MspI CCGG 6 cut(s) 209, 239, 378, 420, 1265, 1410
MspR9I CCNGG 4 cut(s) 75, 209, 239, 1392
MunI CAATTG 1 cut(s) 1377
Mva1269I GAATGC 1 cut(s) 321
MvaI CCWGG 2 cut(s) 75, 1392
MvnI CGCG 1 cut(s) 891
MwoI GCNNNNNNNGC 7 cut(s) 263, 531, 540, 579, 582, 785, 1232
NaeI GCCGGC 2 cut(s) 379, 1266
NciI CCSGG 2 cut(s) 209, 239
NdeI CATATG 1 cut(s) 1492
NdeII GATC 3 cut(s) 124, 398, 1194
NgoMIV GCCGGC 2 cut(s) 377, 1264
NheI GCTAGC 1 cut(s) 470
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 1 cut(s) 1387
NmuCI GTSAC 3 cut(s) 297, 1315, 1342
PaeR7I CTCGAG 1 cut(s) 160
PaqCI CACCTGC 1 cut(s) 1346
PctI GAATGC 1 cut(s) 321
PdiI GCCGGC 2 cut(s) 379, 1266
PfeI GAWTC 1 cut(s) 1276
PkrI GCNGC 7 cut(s) 88, 575, 584, 619, 787, 1228, 1263
PleI GAGTC 2 cut(s) 90, 230
PpsI GAGTC 2 cut(s) 90, 230
Psp6I CCWGG 2 cut(s) 73, 1390
PspGI CCWGG 2 cut(s) 73, 1390
PspN4I GGNNCC 1 cut(s) 1387
PspPI GGNCC 2 cut(s) 282, 758
PspXI VCTCGAGB 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 1 cut(s) 398
PvuII CAGCTG 1 cut(s) 1436
RsaI GTAC 3 cut(s) 634, 854, 1371
RsaNI GTAC 3 cut(s) 633, 853, 1370
SaqAI TTAA 2 cut(s) 141, 1103
SatI GCNGC 7 cut(s) 87, 574, 583, 618, 786, 1227, 1262
Sau3AI GATC 3 cut(s) 124, 398, 1194
Sau96I GGNCC 2 cut(s) 282, 758
ScaI AGTACT 1 cut(s) 1371
SchI GAGTC 2 cut(s) 90, 231
ScrFI CCNGG 4 cut(s) 75, 209, 239, 1392
SduI GDGCHC 1 cut(s) 1390
SfaNI GCATC 9 cut(s) 232, 299, 406, 614, 696, 772, 790, 822, 1324
Sfr274I CTCGAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 282
SlaI CTCGAG 1 cut(s) 160
SmlI CTYRAG 3 cut(s) 160, 475, 577
SmoI CTYRAG 3 cut(s) 160, 475, 577
SpeI ACTAGT 1 cut(s) 710
Sse9I AATT 7 cut(s) 732, 762, 1021, 1029, 1036, 1310, 1377
SsiI CCGC 8 cut(s) 71, 91, 375, 534, 573, 585, 620, 1226
SspI AATATT 1 cut(s) 179
SspMI CTAG 4 cut(s) 227, 471, 711, 1331
StyD4I CCNGG 4 cut(s) 73, 207, 237, 1390
StyI CCWWGG 4 cut(s) 285, 354, 957, 1146
TaaI ACNGT 4 cut(s) 647, 754, 775, 879
TaiI ACGT 2 cut(s) 560, 1218
TaqI TCGA 4 cut(s) 161, 715, 816, 1482
TaqII GACCGA 1 cut(s) 692
TasI AATT 7 cut(s) 732, 762, 1021, 1029, 1036, 1310, 1377
TatI WGTACW 2 cut(s) 852, 1369
TauI GCSGC 2 cut(s) 576, 1229
TfiI GAWTC 1 cut(s) 1276
Tru1I TTAA 2 cut(s) 141, 1103
Tru9I TTAA 2 cut(s) 141, 1103
TscAI CASTG 2 cut(s) 300, 1143
TseFI GTSAC 3 cut(s) 297, 1315, 1342
TseI GCWGC 5 cut(s) 86, 582, 617, 785, 1261
Tsp45I GTSAC 3 cut(s) 297, 1315, 1342
TspDTI ATGAA 3 cut(s) 380, 1080, 1521
TspRI CASTG 2 cut(s) 300, 1143
VpaK11BI GGWCC 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 1353
XhoI CTCGAG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 1017
XspI CTAG 4 cut(s) 227, 471, 711, 1331
ZrmI AGTACT 1 cut(s) 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.