Rorug05G0509900

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000427
Physical Location & Seq
Reverse (-)
69304414 .. 69307055
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0509900.1

Sequence Viewer

Length: 1044 bp
ATGGCCGCGTGCGGTGCCGACACGGCCCGAACCCTCGCTTGCCGCGACGGCCACGCGGCCGCGCAGCTGAAGCTCATCTCCATCGCCGTCATCTTCATCACCAGCGTCGTCGGCATCACCTCCCCTGTCCTCCTCGCCCGTCACTTCCAAGGCAAGCCCGCCTACGACCGTGCTACTCTCATAATCAAGTGCTTCGCCGCCGGCGTCATCCTCTCCACTTCCCTCGTCCACGTCCTCCCCGATGCCTTCGCCGCCCTAGCCGACTGCCACGTGGCCTCCCGCCATCCCTGGAAGGACTACCCCTTCTCCGGTCTCATCACCATGATCGGCGCCATCGCCGCCCTCCTCGTCGACCTCACCGCCAGCGCCCACATGGAGAGCCACAGCACCCACGCCCACGGCCAATACGAGGTCCTTTCGGTGAGCACACAGGAGGAGCTAGGGACCAAGAAGTCGTCGCCGATCGAGTTGAAAGTTGAGTCCGTGGAGGGTGCAGTTAGCGATAGTGGCGGTGGTGATAGCCATAGCCATAGCCATGAGGAGATGATCATTCGGCTGAAGCAGAGATTGGTGTCGCAGGTGTTGGAGATAGGGATAATATTTCACTCGGTGATTATAGGAGTGACGATGGGGATGTCTCAGAACAAGTGCACCATTCGGCCATTGGTGGCTGCGCTTGCGTTTCATCAGATTTTCGAGGGGATGGGCCTCGGCGGCTGCATTGCTCAGGCAGGGTTTACCTTTGGAACAACGGCCTACATGTGCTTCATGTTTGCAGTGACGACCCCAATGGGGATAGTGTTGGGGATGATCTTATTCTCGGTGACAGGTTACGACGATAGTAGCACTAATGCCTTAATCTTGGAGGGGTTGTTGGGGTCTTTCTCTTCAGGTATCCTCATTTACATGGCTCTTGTGGATCTCATAGCACTTGATTTCTTCCACAACAAGATGATGAGCTCTAATTCATGGTTGAGGAAAACTTCATTCACTGCTCTTGTTCTTGGCTCCATCTCCATGTCCATCCTTGCCCTTTGGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

347

Amino Acids

36.91

Weight (kDa)

6.29

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 22 - 344 5.5e-73 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 568
AasI GACNNNNNNGTC 1 cut(s) 451
Acc36I ACCTGC 1 cut(s) 568
AccB1I GGYRCC 2 cut(s) 14, 329
AccI GTMKAC 1 cut(s) 351
AccII CGCG 4 cut(s) 8, 45, 56, 62
AclWI GGATC 1 cut(s) 927
AcoI YGGCCR 5 cut(s) 3, 49, 57, 400, 659
AcuI CTGAAG 3 cut(s) 89, 578, 873
AcvI CACGTG 1 cut(s) 271
AcyI GRCGYC 2 cut(s) 204, 330
AdeI CACNNNGTG 1 cut(s) 610
AfiI CCNNNNNNNGG 3 cut(s) 308, 409, 792
AflIII ACRYGT 1 cut(s) 759
AgsI TTSAA 1 cut(s) 472
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 287
AjuI GAANNNNNNNTTGG 2 cut(s) 551, 583
AluBI AGCT 4 cut(s) 67, 73, 439, 960
AluI AGCT 4 cut(s) 67, 73, 439, 960
Alw21I GWGCWC 3 cut(s) 428, 653, 962
Alw26I GTCTC 2 cut(s) 317, 642
Alw44I GTGCAC 1 cut(s) 649
AlwI GGATC 1 cut(s) 927
ApaLI GTGCAC 1 cut(s) 649
ApeKI GCWGC 3 cut(s) 64, 671, 717
AspLEI GCGC 4 cut(s) 64, 332, 368, 676
AspS9I GGNCC 5 cut(s) 25, 412, 444, 706, 1038
AsuHPI GGTGA 8 cut(s) 91, 109, 310, 349, 433, 527, 622, 835
AvaII GGWCC 2 cut(s) 412, 444
BaeGI GKGCMC 1 cut(s) 653
BanI GGYRCC 2 cut(s) 14, 329
BanII GRGCYC 1 cut(s) 962
BbrPI CACGTG 1 cut(s) 271
Bbv12I GWGCWC 3 cut(s) 428, 653, 962
BbvI GCAGC 3 cut(s) 76, 658, 704
BccI CCATC 7 cut(s) 89, 291, 341, 622, 697, 1019, 1031
BceAI ACGGC 5 cut(s) 39, 64, 71, 415, 768
BciT130I CCWGG 1 cut(s) 289
BciVI GTATCC 1 cut(s) 905
BclI TGATCA 1 cut(s) 546
BcoDI GTCTC 2 cut(s) 317, 642
BfaI CTAG 3 cut(s) 257, 440, 1042
BfoI RGCGCY 2 cut(s) 333, 369
BfuAI ACCTGC 1 cut(s) 568
BfuI GTATCC 1 cut(s) 905
BglI GCCNNNNNGGC 4 cut(s) 23, 48, 714, 1037
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 2 cut(s) 412, 444
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 5 cut(s) 25, 412, 444, 706, 1038
BmiI GGNNCC 4 cut(s) 16, 331, 445, 1009
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 2 cut(s) 123, 232
Bpu10I CCTNAGC 1 cut(s) 726
BsaAI YACGTR 1 cut(s) 271
BsaHI GRCGYC 2 cut(s) 204, 330
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 5 cut(s) 148, 287, 397, 483, 709
BsaWI WCCGGW 1 cut(s) 308
BsaXI ACNNNNNCTCC 6 cut(s) 260, 290, 290, 320, 857, 887
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 409, 792
Bse118I RCCGGY 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 720
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 5 cut(s) 148, 287, 397, 483, 709
BseGI GGATG 6 cut(s) 207, 283, 639, 708, 813, 1023
BseLI CCNNNNNNNGG 3 cut(s) 308, 409, 792
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 2 cut(s) 653, 740
BseRI GAGGAG 4 cut(s) 122, 335, 449, 554
BseSI GKGCMC 1 cut(s) 653
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 3 cut(s) 76, 658, 704
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 4 cut(s) 8, 45, 56, 62
Bsh1285I CGRYCG 3 cut(s) 60, 169, 465
BshNI GGYRCC 2 cut(s) 14, 329
BsiEI CGRYCG 3 cut(s) 60, 169, 465
BsiHKAI GWGCWC 3 cut(s) 428, 653, 962
BsiSI CCGG 2 cut(s) 201, 309
BslFI GGGAC 1 cut(s) 457
BslI CCNNNNNNNGG 3 cut(s) 308, 409, 792
BsmAI GTCTC 2 cut(s) 317, 642
BsmFI GGGAC 1 cut(s) 457
Bso31I GGTCTC 1 cut(s) 317
Bsp1286I GDGCHC 3 cut(s) 428, 653, 962
Bsp143I GATC 5 cut(s) 324, 462, 546, 810, 919
BspCNI CTCAG 2 cut(s) 652, 739
BspFNI CGCG 4 cut(s) 8, 45, 56, 62
BspLI GGNNCC 4 cut(s) 16, 331, 445, 1009
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 1 cut(s) 927
BspT107I GGYRCC 2 cut(s) 14, 329
BspTNI GGTCTC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 720
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 5 cut(s) 148, 287, 397, 483, 709
BssMI GATC 5 cut(s) 324, 462, 546, 810, 919
BssNI GRCGYC 2 cut(s) 204, 330
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 1 cut(s) 892
BstACI GRCGYC 2 cut(s) 204, 330
BstBAI YACGTR 1 cut(s) 271
BstC8I GCNNGC 7 cut(s) 10, 40, 155, 159, 202, 364, 678
BstDEI CTNAG 2 cut(s) 639, 726
BstDSI CCRYGG 2 cut(s) 397, 483
BstF5I GGATG 6 cut(s) 207, 283, 639, 708, 813, 1023
BstFNI CGCG 4 cut(s) 8, 45, 56, 62
BstH2I RGCGCY 2 cut(s) 333, 369
BstHHI GCGC 4 cut(s) 64, 332, 368, 676
BstKTI GATC 5 cut(s) 327, 465, 549, 813, 922
BstMAI GTCTC 2 cut(s) 317, 642
BstMBI GATC 5 cut(s) 324, 462, 546, 810, 919
BstMCI CGRYCG 3 cut(s) 60, 169, 465
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 1 cut(s) 287
BstSLI GKGCMC 1 cut(s) 653
BstUI CGCG 4 cut(s) 8, 45, 56, 62
BstV1I GCAGC 3 cut(s) 76, 658, 704
BstX2I RGATCY 1 cut(s) 919
BstYI RGATCY 1 cut(s) 919
BstZI CGGCCG 1 cut(s) 57
BsuI GTATCC 1 cut(s) 905
BtgI CCRYGG 2 cut(s) 397, 483
BtgZI GCGATG 2 cut(s) 67, 319
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 6 cut(s) 207, 283, 639, 708, 813, 1023
BtsI GCAGTG 2 cut(s) 783, 990
BtsIMutI CAGTG 2 cut(s) 783, 990
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 7 cut(s) 10, 40, 155, 159, 202, 364, 678
CciNI GCGGCCGC 1 cut(s) 57
CfoI GCGC 4 cut(s) 64, 332, 368, 676
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 5 cut(s) 25, 412, 444, 706, 1038
CseI GACGC 2 cut(s) 94, 193
CviAII CATG 8 cut(s) 322, 373, 536, 760, 769, 907, 969, 1018
DdeI CTNAG 2 cut(s) 639, 726
DinI GGCGCC 1 cut(s) 331
DpnI GATC 5 cut(s) 326, 464, 548, 812, 921
DpnII GATC 5 cut(s) 324, 462, 546, 810, 919
DraIII CACNNNGTG 1 cut(s) 610
DrdI GACNNNNNNGTC 1 cut(s) 451
DseDI GACNNNNNNGTC 1 cut(s) 451
EaeI YGGCCR 5 cut(s) 3, 49, 57, 400, 659
EagI CGGCCG 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 892
EarI CTCTTC 1 cut(s) 892
Ecl136II GAGCTC 1 cut(s) 960
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 962
Eco31I GGTCTC 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 412, 444
Eco52I CGGCCG 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 960
Eco57I CTGAAG 3 cut(s) 89, 578, 873
Eco72I CACGTG 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 960
EcoO109I RGGNCCY 1 cut(s) 412
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 962
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 8 cut(s) 325, 376, 539, 763, 772, 910, 972, 1021
FaqI GGGAC 1 cut(s) 457
FatI CATG 8 cut(s) 321, 372, 535, 759, 768, 906, 968, 1017
FauI CCCGC 2 cut(s) 166, 287
FbaI TGATCA 1 cut(s) 546
FblI GTMKAC 1 cut(s) 351
FokI GGATG 6 cut(s) 194, 270, 646, 715, 820, 1010
FriOI GRGCYC 1 cut(s) 962
FspBI CTAG 3 cut(s) 257, 440, 1042
GlaI GCGC 4 cut(s) 63, 331, 367, 675
HaeII RGCGCY 2 cut(s) 333, 369
HapII CCGG 2 cut(s) 201, 309
HgaI GACGC 2 cut(s) 94, 193
HhaI GCGC 4 cut(s) 64, 332, 368, 676
Hin1I GRCGYC 2 cut(s) 204, 330
Hin1II CATG 8 cut(s) 325, 376, 539, 763, 772, 910, 972, 1021
Hin6I GCGC 4 cut(s) 62, 330, 366, 674
HinP1I GCGC 4 cut(s) 62, 330, 366, 674
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 1 cut(s) 479
HpaII CCGG 2 cut(s) 201, 309
HphI GGTGA 8 cut(s) 91, 109, 310, 349, 433, 527, 622, 835
Hpy166II GTNNAC 4 cut(s) 229, 352, 651, 738
Hpy188I TCNGA 2 cut(s) 642, 690
Hpy8I GTNNAC 4 cut(s) 229, 352, 651, 738
Hpy99I CGWCG 6 cut(s) 50, 110, 113, 353, 460, 839
HpyAV CCTTC 3 cut(s) 256, 286, 313
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 2 cut(s) 231, 270
HpyCH4V TGCA 4 cut(s) 494, 651, 720, 776
HpyF3I CTNAG 2 cut(s) 639, 726
HpySE526I ACGT 2 cut(s) 231, 270
Hsp92I GRCGYC 2 cut(s) 204, 330
Hsp92II CATG 8 cut(s) 325, 376, 539, 763, 772, 910, 972, 1021
HspAI GCGC 4 cut(s) 62, 330, 366, 674
KasI GGCGCC 1 cut(s) 329
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 546
Kzo9I GATC 5 cut(s) 324, 462, 546, 810, 919
LmnI GCTCC 2 cut(s) 436, 1013
Lsp1109I GCAGC 3 cut(s) 76, 658, 704
LweI GCATC 2 cut(s) 123, 232
MaeI CTAG 3 cut(s) 257, 440, 1042
MaeII ACGT 2 cut(s) 231, 270
MaeIII GTNAC 5 cut(s) 140, 622, 778, 823, 830
MalI GATC 5 cut(s) 326, 464, 548, 812, 921
MboI GATC 5 cut(s) 324, 462, 546, 810, 919
MboII GAAGA 3 cut(s) 85, 879, 931
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 3 cut(s) 428, 653, 962
MluCI AATT 1 cut(s) 964
Mly113I GGCGCC 1 cut(s) 330
MlyI GAGTC 1 cut(s) 488
MmeI TCCRAC 1 cut(s) 564
MreI CGCCGGCG 1 cut(s) 200
MroNI GCCGGC 1 cut(s) 200
MseI TTAA 1 cut(s) 857
MslI CAYNNNNRTG 4 cut(s) 320, 534, 905, 1016
MspA1I CMGCKG 1 cut(s) 67
MspI CCGG 2 cut(s) 201, 309
MspR9I CCNGG 1 cut(s) 289
MvaI CCWGG 1 cut(s) 289
MvnI CGCG 4 cut(s) 8, 45, 56, 62
NaeI GCCGGC 1 cut(s) 202
NarI GGCGCC 1 cut(s) 330
NdeII GATC 5 cut(s) 324, 462, 546, 810, 919
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 8 cut(s) 325, 376, 539, 763, 772, 910, 972, 1021
NlaIV GGNNCC 4 cut(s) 16, 331, 445, 1009
NmeAIII GCCGAG 1 cut(s) 690
NmuCI GTSAC 4 cut(s) 140, 622, 778, 823
NotI GCGGCCGC 1 cut(s) 57
NspI RCATGY 1 cut(s) 763
PaqCI CACCTGC 1 cut(s) 568
PciI ACATGT 1 cut(s) 759
PcsI WCGNNNNNNNCGW 4 cut(s) 42, 201, 237, 405
PdiI GCCGGC 1 cut(s) 202
Ple19I CGATCG 1 cut(s) 465
PleI GAGTC 1 cut(s) 487
PluTI GGCGCC 1 cut(s) 333
PmaCI CACGTG 1 cut(s) 271
PmlI CACGTG 1 cut(s) 271
PpsI GAGTC 1 cut(s) 487
Ppu21I YACGTR 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 412
PscI ACATGT 1 cut(s) 759
Psp124BI GAGCTC 1 cut(s) 962
Psp5II RGGWCCY 1 cut(s) 412
Psp6I CCWGG 1 cut(s) 287
PspCI CACGTG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 4 cut(s) 16, 331, 445, 1009
PspPI GGNCC 5 cut(s) 25, 412, 444, 706, 1038
PspPPI RGGWCCY 1 cut(s) 412
PsuI RGATCY 1 cut(s) 919
PvuI CGATCG 1 cut(s) 465
PvuII CAGCTG 1 cut(s) 67
RseI CAYNNNNRTG 4 cut(s) 320, 534, 905, 1016
SacI GAGCTC 1 cut(s) 962
SalI GTCGAC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 857
Sau3AI GATC 5 cut(s) 324, 462, 546, 810, 919
Sau96I GGNCC 5 cut(s) 25, 412, 444, 706, 1038
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 3 cut(s) 428, 653, 962
SfaNI GCATC 2 cut(s) 123, 232
SfoI GGCGCC 1 cut(s) 331
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 2 cut(s) 412, 444
SmiMI CAYNNNNRTG 4 cut(s) 320, 534, 905, 1016
Sse9I AATT 1 cut(s) 964
SspDI GGCGCC 1 cut(s) 329
SspI AATATT 1 cut(s) 600
SspMI CTAG 3 cut(s) 257, 440, 1042
SstI GAGCTC 1 cut(s) 962
StyD4I CCNGG 1 cut(s) 287
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 2 cut(s) 234, 273
TaqI TCGA 3 cut(s) 351, 465, 696
TasI AATT 1 cut(s) 964
TauI GCSGC 8 cut(s) 8, 45, 59, 62, 200, 254, 341, 717
Tru1I TTAA 1 cut(s) 857
Tru9I TTAA 1 cut(s) 857
TscAI CASTG 2 cut(s) 783, 997
TseFI GTSAC 4 cut(s) 140, 622, 778, 823
TseI GCWGC 3 cut(s) 64, 671, 717
Tsp45I GTSAC 4 cut(s) 140, 622, 778, 823
TspDTI ATGAA 5 cut(s) 85, 674, 757, 957, 975
TspGWI ACGGA 1 cut(s) 472
TspRI CASTG 2 cut(s) 783, 997
VneI GTGCAC 1 cut(s) 649
VpaK11BI GGWCC 2 cut(s) 412, 444
XceI RCATGY 1 cut(s) 763
XcmI CCANNNNNNNNNTGG 2 cut(s) 370, 661
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 3 cut(s) 257, 440, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.