Rh6DG019900

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000427
Physical Location & Seq
Reverse (-)
1779175 .. 1782057
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG019900.1

Sequence Viewer

Length: 1320 bp
ATGTCTAAATCTTATAACCAACTAACTTCCAGACAAGTAGTTCAGTTCTACCTTCAAGAAATCCTCAGGCTCAACCCAGTTCTTAACGGAGTCATAGAGGTGAACCCGGATGCACTTTACCAAGCTGACAAGGCTGACTATCAGCGAAAGGCTAAGGCCCCTGGTTATTATTCCGGCTTGCATGGCATTCCTATTTTGCTCAAGGATAACATTGGAACCAGAGATAAGCTGAACACTACTGCTGGCTCTTTTGCGCTGCTCGGCTCTGTCGTGCCCCGGGATGCTGGCGTGGTGACCAAGTTGAGGAGCGCTGGAGTCATTATCTTGGGGAAGGCTAGCTTCAGTGAGTGGGCTCACGTTAGGTCTCTCACGTCACCCCCAGGTTTCAGCCCCAGAGGTGGCCAAGGAAAGAACCCTTATGTACTATCTGCAAGTCCATGTGGATCAAGCAGTGGATCATCAATATCAGTTGCAGCAAATTTGGTAGCTGTATCTCTTGTTACTCCCAGACAAGACACCATTGGACCCATCTGCAGGACTGTGACAGATGCTGTTTACGTTCTTGATGCGATTGTAGGCTATGACAACAAGGATCAAGCAACCAAAGAAGCATCGAAATACATTCCACGCGGTGGGTATAAGCAGTTCCTTGGGGCTTATGGGCTCAAGGGAAAGAGACTGGGAATAGTGAGGAATCCCTTCTTCACCTCTGGGAGTGGATCTCTCCAAATTCAAGCATTTGAGCATCATTTCCAAACGCTAAGGCAAGGAGGTGCTGTTTTGGTAGACCATCTGGAAATAGCAAACATTGATACTATCTTGAATTTTAATTTGAGTGGAGAAGCAATAGCAACAGTGGCTGAGTTCAAACTAGCCATAAATGCATATCTAAAAGACCTTGTGGTGTCCCCAGTTCGATCTTTGGCAGATATCATAGCCTTCAACTTGAAGTTTTCTGGTGTGGAAATGATCAAGGAATTTGGCCAAGACATTTTTCTTGCAGCACAAGCCACAAATGGGATAGGCAACAATGAGAAGGCAGCATTGTCATATTTAGCAAAGCTGACAAAAGATGGATTTGAGAAACTAATGACATACAACAGGCTAGATGCATTGGTGACTCCCGATGCAGATATTGCTCCTGTACTTGCACTTGGGGGATTCCCAGGCATTAGTGTCCCTGCTGGATATGACAAGAAGGGTGTGCCTTTCGGCATAACTTTTGGGGGCCTAAAGGGATCTGAACCAAAGCTTATACAGATTGCCTACGGTTTCGAGCAAGCTACTAAGATTAGAAAGCCTCCCACATTTCTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

439

Amino Acids

46.99

Weight (kDa)

9.36

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 13 - 166 2.7e-33 Amidase
Amidase PF01425 170 - 382 7.8e-08 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 15
AccB7I CCANNNNNTGG 1 cut(s) 632
AccI GTMKAC 1 cut(s) 786
AccII CGCG 1 cut(s) 630
AciI CCGC 1 cut(s) 630
AclWI GGATC 5 cut(s) 451, 463, 600, 727, 1246
AcoI YGGCCR 2 cut(s) 400, 982
AcsI RAATTY 4 cut(s) 478, 729, 823, 977
AcuI CTGAAG 1 cut(s) 325
AdeI CACNNNGTG 1 cut(s) 632
AfaI GTAC 2 cut(s) 423, 1146
AfeI AGCGCT 1 cut(s) 310
AfiI CCNNNNNNNGG 5 cut(s) 303, 398, 534, 632, 1017
AgsI TTSAA 6 cut(s) 56, 734, 823, 868, 943, 949
AjiI CACGTC 1 cut(s) 372
AjnI CCWGG 3 cut(s) 160, 379, 1165
AjuI GAANNNNNNNTTGG 2 cut(s) 978, 1010
AluBI AGCT 7 cut(s) 125, 229, 339, 488, 1063, 1252, 1283
AluI AGCT 7 cut(s) 125, 229, 339, 488, 1063, 1252, 1283
Alw26I GTCTC 2 cut(s) 369, 670
AlwI GGATC 5 cut(s) 451, 463, 600, 727, 1246
AlwNI CAGNNNCTG 2 cut(s) 551, 860
Ama87I CYCGRG 1 cut(s) 276
Aor51HI AGCGCT 1 cut(s) 310
AoxI GGCC 4 cut(s) 156, 400, 982, 1228
ApeKI GCWGC 4 cut(s) 256, 473, 1001, 1040
ApoI RAATTY 4 cut(s) 478, 729, 823, 977
Asp700I GAANNNNTTC 1 cut(s) 698
AspLEI GCGC 2 cut(s) 256, 311
AspS9I GGNCC 3 cut(s) 157, 524, 1228
AsuC2I CCSGG 3 cut(s) 107, 277, 278
AsuHPI GGTGA 5 cut(s) 112, 304, 366, 697, 1129
AsuNHI GCTAGC 1 cut(s) 335
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 1 cut(s) 524
AxyI CCTNAGG 1 cut(s) 65
BaeGI GKGCMC 1 cut(s) 276
BalI TGGCCA 2 cut(s) 402, 984
BanII GRGCYC 2 cut(s) 355, 666
BbvI GCAGC 4 cut(s) 243, 485, 1013, 1052
BccI CCATC 3 cut(s) 536, 798, 1067
BciT130I CCWGG 3 cut(s) 162, 381, 1167
BclI TGATCA 1 cut(s) 969
BcnI CCSGG 3 cut(s) 107, 277, 278
BcoDI GTCTC 2 cut(s) 369, 670
BfaI CTAG 3 cut(s) 336, 872, 1106
BfmI CTRYAG 1 cut(s) 532
BfoI RGCGCY 1 cut(s) 312
BisI GCNGC 4 cut(s) 257, 474, 1002, 1041
BlsI GCNGC 4 cut(s) 258, 475, 1003, 1042
Bme1390I CCNGG 6 cut(s) 107, 162, 277, 278, 381, 1167
Bme18I GGWCC 1 cut(s) 524
BmeT110I CYCGRG 1 cut(s) 276
BmgBI CACGTC 1 cut(s) 372
BmgT120I GGNCC 3 cut(s) 157, 524, 1228
BmiI GGNNCC 4 cut(s) 159, 217, 526, 1229
BmrFI CCNGG 6 cut(s) 107, 162, 277, 278, 381, 1167
BmrI ACTGGG 3 cut(s) 71, 689, 905
BmsI GCATC 8 cut(s) 100, 271, 538, 556, 620, 754, 1099, 1117
BmtI GCTAGC 1 cut(s) 339
BmuI ACTGGG 3 cut(s) 71, 689, 905
BplI GAGNNNNNCTC 2 cut(s) 706, 738
BpmI CTGGAG 1 cut(s) 333
Bpu10I CCTNAGC 2 cut(s) 153, 761
BpuEI CTTGAG 2 cut(s) 185, 650
BpuMI CCSGG 3 cut(s) 107, 277, 278
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 7 cut(s) 160, 275, 276, 379, 403, 649, 1165
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 5 cut(s) 303, 398, 534, 632, 1017
Bse1I ACTGG 3 cut(s) 77, 684, 911
Bse21I CCTNAGG 1 cut(s) 65
BseBI CCWGG 3 cut(s) 162, 381, 1167
BseDI CCNNGG 7 cut(s) 160, 275, 276, 379, 403, 649, 1165
BseGI GGATG 2 cut(s) 115, 286
BseLI CCNNNNNNNGG 5 cut(s) 303, 398, 534, 632, 1017
BseMII CTCAG 2 cut(s) 79, 852
BseNI ACTGG 3 cut(s) 77, 684, 911
BseRI GAGGAG 1 cut(s) 319
BseSI GKGCMC 1 cut(s) 276
BseXI GCAGC 4 cut(s) 243, 485, 1013, 1052
Bsh1236I CGCG 1 cut(s) 630
BshFI GGCC 4 cut(s) 158, 402, 984, 1230
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 3 cut(s) 107, 174, 277
BslFI GGGAC 2 cut(s) 892, 1163
BslI CCNNNNNNNGG 5 cut(s) 303, 398, 534, 632, 1017
BsmAI GTCTC 2 cut(s) 369, 670
BsmFI GGGAC 2 cut(s) 892, 1163
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 4 cut(s) 158, 402, 984, 1230
Bso31I GGTCTC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 276
Bsp1286I GDGCHC 3 cut(s) 276, 355, 666
Bsp143I GATC 7 cut(s) 443, 455, 592, 719, 917, 969, 1238
BspACI CCGC 1 cut(s) 630
BspANI GGCC 4 cut(s) 158, 402, 984, 1230
BspCNI CTCAG 2 cut(s) 78, 853
BspFNI CGCG 1 cut(s) 630
BspLI GGNNCC 4 cut(s) 159, 217, 526, 1229
BspMAI CTGCAG 1 cut(s) 536
BspOI GCTAGC 1 cut(s) 339
BspPI GGATC 5 cut(s) 451, 463, 600, 727, 1246
BspTNI GGTCTC 1 cut(s) 369
BsrI ACTGG 3 cut(s) 77, 684, 911
BssECI CCNNGG 7 cut(s) 160, 275, 276, 379, 403, 649, 1165
BssMI GATC 7 cut(s) 443, 455, 592, 719, 917, 969, 1238
BssT1I CCWWGG 2 cut(s) 403, 649
Bst2UI CCWGG 3 cut(s) 162, 381, 1167
Bst4CI ACNGT 3 cut(s) 541, 856, 1271
BstAPI GCANNNNNTGC 1 cut(s) 1136
BstC8I GCNNGC 5 cut(s) 179, 244, 286, 337, 1281
BstDEI CTNAG 5 cut(s) 65, 153, 761, 861, 1287
BstEII GGTNACC 1 cut(s) 292
BstF5I GGATG 2 cut(s) 115, 286
BstFNI CGCG 1 cut(s) 630
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 2 cut(s) 256, 311
BstKTI GATC 7 cut(s) 446, 458, 595, 722, 920, 972, 1241
BstMAI GTCTC 2 cut(s) 369, 670
BstMBI GATC 7 cut(s) 443, 455, 592, 719, 917, 969, 1238
BstMWI GCNNNNNNNGC 6 cut(s) 131, 183, 857, 881, 1007, 1136
BstNI CCWGG 3 cut(s) 162, 381, 1167
BstPI GGTNACC 1 cut(s) 292
BstSCI CCNGG 6 cut(s) 105, 160, 275, 276, 379, 1165
BstSFI CTRYAG 1 cut(s) 532
BstSLI GKGCMC 1 cut(s) 276
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 4 cut(s) 243, 485, 1013, 1052
BstX2I RGATCY 2 cut(s) 719, 1238
BstYI RGATCY 2 cut(s) 719, 1238
Bsu36I CCTNAGG 1 cut(s) 65
BsuRI GGCC 4 cut(s) 158, 402, 984, 1230
BtrI CACGTC 1 cut(s) 372
BtsCI GGATG 2 cut(s) 115, 286
BtsI GCAGTG 1 cut(s) 457
BtsIMutI CAGTG 3 cut(s) 349, 457, 861
Cac8I GCNNGC 5 cut(s) 179, 244, 286, 337, 1281
CaiI CAGNNNCTG 2 cut(s) 551, 860
CfoI GCGC 2 cut(s) 256, 311
Cfr13I GGNCC 3 cut(s) 157, 524, 1228
Cfr9I CCCGGG 1 cut(s) 276
Csp6I GTAC 2 cut(s) 422, 1145
CviAII CATG 2 cut(s) 182, 438
CviQI GTAC 2 cut(s) 422, 1145
DdeI CTNAG 5 cut(s) 65, 153, 761, 861, 1287
DpnI GATC 7 cut(s) 445, 457, 594, 721, 919, 971, 1240
DpnII GATC 7 cut(s) 443, 455, 592, 719, 917, 969, 1238
DraIII CACNNNGTG 1 cut(s) 632
EaeI YGGCCR 2 cut(s) 400, 982
Eco130I CCWWGG 2 cut(s) 403, 649
Eco24I GRGCYC 2 cut(s) 355, 666
Eco31I GGTCTC 1 cut(s) 369
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 1 cut(s) 524
Eco47III AGCGCT 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 276
Eco91I GGTNACC 1 cut(s) 292
EcoO109I RGGNCCY 2 cut(s) 157, 1228
EcoO65I GGTNACC 1 cut(s) 292
EcoRII CCWGG 3 cut(s) 160, 379, 1165
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 2 cut(s) 403, 649
EcoT22I ATGCAT 2 cut(s) 886, 1114
EcoT38I GRGCYC 2 cut(s) 355, 666
ErhI CCWWGG 2 cut(s) 403, 649
FaeI CATG 2 cut(s) 185, 441
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 2 cut(s) 892, 1163
FatI CATG 2 cut(s) 181, 437
FbaI TGATCA 1 cut(s) 969
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 4 cut(s) 257, 474, 1002, 1041
FokI GGATG 2 cut(s) 122, 293
FriOI GRGCYC 2 cut(s) 355, 666
Fsp4HI GCNGC 4 cut(s) 257, 474, 1002, 1041
FspBI CTAG 3 cut(s) 336, 872, 1106
GlaI GCGC 2 cut(s) 255, 310
GluI GCNGC 4 cut(s) 257, 474, 1002, 1041
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 4 cut(s) 158, 402, 984, 1230
HapII CCGG 3 cut(s) 107, 174, 277
HhaI GCGC 2 cut(s) 256, 311
Hin1II CATG 2 cut(s) 185, 441
Hin6I GCGC 2 cut(s) 254, 309
HinP1I GCGC 2 cut(s) 254, 309
HindIII AAGCTT 1 cut(s) 1250
HinfI GANTC 5 cut(s) 90, 315, 694, 1120, 1161
HpaII CCGG 3 cut(s) 107, 174, 277
HphI GGTGA 5 cut(s) 112, 304, 366, 697, 1129
Hpy166II GTNNAC 3 cut(s) 103, 556, 787
Hpy188I TCNGA 1 cut(s) 1243
Hpy188III TCNNGA 6 cut(s) 30, 56, 563, 794, 820, 1124
Hpy8I GTNNAC 3 cut(s) 103, 556, 787
HpyAV CCTTC 6 cut(s) 62, 325, 709, 949, 1030, 1192
HpyCH4III ACNGT 3 cut(s) 541, 856, 1271
HpyCH4IV ACGT 3 cut(s) 357, 371, 558
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 183, 857, 881, 1007, 1136
HpyF3I CTNAG 5 cut(s) 65, 153, 761, 861, 1287
HpySE526I ACGT 3 cut(s) 357, 371, 558
Hsp92II CATG 2 cut(s) 185, 441
HspAI GCGC 2 cut(s) 254, 309
Ksp22I TGATCA 1 cut(s) 969
Kzo9I GATC 7 cut(s) 443, 455, 592, 719, 917, 969, 1238
LmnI GCTCC 2 cut(s) 306, 1144
Lsp1109I GCAGC 4 cut(s) 243, 485, 1013, 1052
LweI GCATC 8 cut(s) 100, 271, 538, 556, 620, 754, 1099, 1117
MaeI CTAG 3 cut(s) 336, 872, 1106
MaeII ACGT 3 cut(s) 357, 371, 558
MaeIII GTNAC 5 cut(s) 292, 372, 499, 541, 1117
MalI GATC 7 cut(s) 445, 457, 594, 721, 919, 971, 1240
MboI GATC 7 cut(s) 443, 455, 592, 719, 917, 969, 1238
MboII GAAGA 1 cut(s) 694
MflI RGATCY 2 cut(s) 719, 1238
MhlI GDGCHC 3 cut(s) 276, 355, 666
MlsI TGGCCA 2 cut(s) 402, 984
MluCI AATT 5 cut(s) 478, 729, 823, 829, 977
MluNI TGGCCA 2 cut(s) 402, 984
MlyI GAGTC 3 cut(s) 99, 324, 1114
MnlI CCTC 8 cut(s) 74, 91, 297, 389, 684, 718, 764, 1311
Mox20I TGGCCA 2 cut(s) 402, 984
Mph1103I ATGCAT 2 cut(s) 886, 1114
MroXI GAANNNNTTC 1 cut(s) 698
MscI TGGCCA 2 cut(s) 402, 984
MseI TTAA 2 cut(s) 84, 828
MslI CAYNNNNRTG 1 cut(s) 98
Msp20I TGGCCA 2 cut(s) 402, 984
MspI CCGG 3 cut(s) 107, 174, 277
MspR9I CCNGG 6 cut(s) 107, 162, 277, 278, 381, 1167
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 3 cut(s) 162, 381, 1167
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 6 cut(s) 131, 183, 857, 881, 1007, 1136
NciI CCSGG 3 cut(s) 107, 277, 278
NdeII GATC 7 cut(s) 443, 455, 592, 719, 917, 969, 1238
NheI GCTAGC 1 cut(s) 335
NlaIII CATG 2 cut(s) 185, 441
NlaIV GGNNCC 4 cut(s) 159, 217, 526, 1229
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 4 cut(s) 292, 372, 541, 1117
NsiI ATGCAT 2 cut(s) 886, 1114
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 698
PfeI GAWTC 2 cut(s) 694, 1161
PflMI CCANNNNNTGG 1 cut(s) 632
PkrI GCNGC 4 cut(s) 258, 475, 1003, 1042
PleI GAGTC 3 cut(s) 98, 323, 1114
PpsI GAGTC 3 cut(s) 98, 323, 1114
PsiI TTATAA 1 cut(s) 15
Psp6I CCWGG 3 cut(s) 160, 379, 1165
PspEI GGTNACC 1 cut(s) 292
PspGI CCWGG 3 cut(s) 160, 379, 1165
PspN4I GGNNCC 4 cut(s) 159, 217, 526, 1229
PspPI GGNCC 3 cut(s) 157, 524, 1228
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PstI CTGCAG 1 cut(s) 536
PstNI CAGNNNCTG 2 cut(s) 551, 860
PsuI RGATCY 2 cut(s) 719, 1238
RsaI GTAC 2 cut(s) 423, 1146
RsaNI GTAC 2 cut(s) 422, 1145
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 2 cut(s) 84, 828
SatI GCNGC 4 cut(s) 257, 474, 1002, 1041
Sau3AI GATC 7 cut(s) 443, 455, 592, 719, 917, 969, 1238
Sau96I GGNCC 3 cut(s) 157, 524, 1228
SchI GAGTC 3 cut(s) 99, 324, 1114
ScrFI CCNGG 6 cut(s) 107, 162, 277, 278, 381, 1167
SduI GDGCHC 3 cut(s) 276, 355, 666
SfaNI GCATC 8 cut(s) 100, 271, 538, 556, 620, 754, 1099, 1117
SfcI CTRYAG 1 cut(s) 532
SinI GGWCC 1 cut(s) 524
SmaI CCCGGG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 2 cut(s) 200, 665
SmoI CTYRAG 2 cut(s) 200, 665
Sse9I AATT 5 cut(s) 478, 729, 823, 829, 977
SsiI CCGC 1 cut(s) 630
SspMI CTAG 3 cut(s) 336, 872, 1106
StyD4I CCNGG 6 cut(s) 105, 160, 275, 276, 379, 1165
StyI CCWWGG 2 cut(s) 403, 649
TaaI ACNGT 3 cut(s) 541, 856, 1271
TaiI ACGT 3 cut(s) 360, 374, 561
TaqI TCGA 3 cut(s) 614, 916, 1275
TasI AATT 5 cut(s) 478, 729, 823, 829, 977
TatI WGTACW 2 cut(s) 421, 1144
TfiI GAWTC 2 cut(s) 694, 1161
Tru1I TTAA 2 cut(s) 84, 828
Tru9I TTAA 2 cut(s) 84, 828
TscAI CASTG 3 cut(s) 349, 457, 861
TseFI GTSAC 4 cut(s) 292, 372, 541, 1117
TseI GCWGC 4 cut(s) 256, 473, 1001, 1040
Tsp45I GTSAC 4 cut(s) 292, 372, 541, 1117
TspGWI ACGGA 1 cut(s) 102
TspMI CCCGGG 1 cut(s) 276
TspRI CASTG 3 cut(s) 349, 457, 861
Van91I CCANNNNNTGG 1 cut(s) 632
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 4 cut(s) 478, 729, 823, 977
XmaI CCCGGG 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 698
XspI CTAG 3 cut(s) 336, 872, 1106
Zsp2I ATGCAT 2 cut(s) 886, 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.