Rorug05G0510000

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000427
Physical Location & Seq
Reverse (-)
69343369 .. 69345375
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0510000.1

Sequence Viewer

Length: 1197 bp
ATGGGACTAAAGGAGGCTGTAATTCGAAGCTATGATGGTGAAGCGGATAGAGCTAGAGTGGAAGACCTTGAACGAAGATGCGAGGTAGGACCAACGGAGCGAGTATATCTCTTCACAGACACTATGGGTGACCCCATATGTAGGATCCGGAACAGTCCGATGCACAAGATGCTGGTGGCCGAGTTGGACAACCAGTTGGTTGGAGTCATTCAAGGCTCGATAAAGTTGGTGACGGTTCATAATAAGCCGCCGCCTAGAGATCATGAGGCCAATCTGGCCAGAGTGGGCTACATCCTCGGCCTGAGGGTCGCACCATTCCATCGTCAAAAAGGGATTGGTTCCAGACTGGTGTGTTGTTTAGAGGATTGGTTCGTAGCCAACGACGTAGATTACTCATACATGGCCACCGAGAAAGACAACGAAGCTTCAGTCAGGCTTTTCATTGAGAAGTTTGGCTACATCAAGTTCAGGACCCCTGCAATTCTGGTAAACCCGGTTCGTTGCCGGCCCTGCCACGTCTCTTCCAACGTTGAAATCGCAAAGCTCAAGGTAGAAGAAGCCGAGTTTCTGTACAGAAAGTGTATGGCTTCCACTGATTTCTTTCCTCATGACATAAACAAAGTGCTTGGGAACAAACTGAGCTTGGGGACTTGGGTGGCCTATCCAAGAGGAGAGTTTGGCAATTTTGAATCAAATGGGCAAGCTCTACCAAAAAGTTGGGCTATGATTAGTGTATGGAACAGCGGTGAGGTTTTCAAATTGAGGCTAGGGAAGGCACCTCTGTCTTGTCTGTTGTATGCAAAAAGCTGGAGACTGATGGATAGGTTTTTGCCTTGCTTTAAATTACTGCCTTCCATGCCTGATTTTTTCAACCCTTTTGGGTTTTACTTCATGTATGGGGTATACCATGAAGGACCATTGTCAGGGAAACTGGTGAGGAATTTGTGCCACTTTGTGCACAACATGGCTAGTACTACAACTAAGAACTTCTGCTGCAAGGTCGTAGTAACAGAAGTTGGGGGTTATGACTCAGTACTAAGACATCACATCCCACATTGGAAGTTGCTCTCTTGCTCTGAGGATTTGTGGTGCATAAAAGCCATGAAGAAAAATGAAGCAGACAGAACCAGCCTTCATGAATTGACTAAAACCCAACAAATAAATAAAGCTCTTTTTGTTGACCCAAGAGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

398

Amino Acids

45.51

Weight (kDa)

8.82

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 43 - 153 3.7e-14 Acetyltransferase (GNAT) family
Acetyltransf_7 PF13508 55 - 154 1.2e-06 Acetyltransferase (GNAT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 775
AccI GTMKAC 1 cut(s) 903
AccIII TCCGGA 1 cut(s) 147
AciI CCGC 4 cut(s) 44, 248, 251, 744
AclI AACGTT 1 cut(s) 528
AclWI GGATC 2 cut(s) 139, 152
AcoI YGGCCR 3 cut(s) 177, 276, 402
AcsI RAATTY 1 cut(s) 940
AcuI CTGAAG 1 cut(s) 411
AdeI CACNNNGTG 1 cut(s) 955
AfaI GTAC 3 cut(s) 572, 973, 1035
AfiI CCNNNNNNNGG 3 cut(s) 141, 923, 1189
AgsI TTSAA 6 cut(s) 71, 212, 533, 689, 757, 871
AjiI CACGTC 1 cut(s) 517
AluBI AGCT 8 cut(s) 30, 53, 425, 544, 642, 704, 807, 1169
AluI AGCT 8 cut(s) 30, 53, 425, 544, 642, 704, 807, 1169
Alw21I GWGCWC 1 cut(s) 960
Alw26I GTCTC 2 cut(s) 523, 805
Alw44I GTGCAC 1 cut(s) 956
AlwI GGATC 2 cut(s) 139, 152
Aor13HI TCCGGA 1 cut(s) 147
AoxI GGCC 7 cut(s) 177, 267, 276, 298, 402, 506, 657
ApaLI GTGCAC 1 cut(s) 956
ApeKI GCWGC 1 cut(s) 993
ApoI RAATTY 1 cut(s) 940
ArsI GACNNNNNNTTYG 2 cut(s) 414, 446
AspS9I GGNCC 4 cut(s) 89, 471, 507, 914
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 5 cut(s) 50, 140, 241, 758, 946
AsuII TTCGAA 1 cut(s) 25
AvaII GGWCC 3 cut(s) 89, 471, 914
AxyI CCTNAGG 1 cut(s) 302
BaeGI GKGCMC 1 cut(s) 960
BalI TGGCCA 2 cut(s) 278, 404
BamHI GGATCC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 775
BarI GAAGNNNNNNTAC 2 cut(s) 440, 472
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 960
BbvI GCAGC 1 cut(s) 980
BccI CCATC 3 cut(s) 29, 327, 811
BcnI CCSGG 1 cut(s) 494
BcoDI GTCTC 2 cut(s) 523, 805
BfaI CTAG 4 cut(s) 54, 255, 767, 969
BglI GCCNNNNNGGC 1 cut(s) 275
BisI GCNGC 3 cut(s) 248, 251, 994
BlsI GCNGC 3 cut(s) 249, 252, 995
BmcAI AGTACT 2 cut(s) 973, 1035
Bme1390I CCNGG 1 cut(s) 494
Bme18I GGWCC 3 cut(s) 89, 471, 914
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 4 cut(s) 89, 471, 507, 914
BmiI GGNNCC 4 cut(s) 146, 340, 473, 777
BmrFI CCNGG 1 cut(s) 494
BmsI GCATC 3 cut(s) 68, 150, 159
BoxI GACNNNNGTC 1 cut(s) 919
BpiI GAAGAC 1 cut(s) 69
BplI GAGNNNNNCTC 2 cut(s) 93, 125
BpmI CTGGAG 1 cut(s) 829
Bpu14I TTCGAA 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 494
BsaJI CCNNGG 1 cut(s) 295
BsaWI WCCGGW 1 cut(s) 147
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 923, 1189
Bse118I RCCGGY 1 cut(s) 504
Bse1I ACTGG 3 cut(s) 193, 351, 936
Bse21I CCTNAGG 1 cut(s) 302
BseAI TCCGGA 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 2 cut(s) 291, 1047
BseLI CCNNNNNNNGG 3 cut(s) 141, 923, 1189
BseMII CTCAG 4 cut(s) 293, 629, 1044, 1068
BseNI ACTGG 3 cut(s) 193, 351, 936
BseRI GAGGAG 1 cut(s) 684
BseSI GKGCMC 1 cut(s) 960
BseXI GCAGC 1 cut(s) 980
BshFI GGCC 7 cut(s) 179, 269, 278, 300, 404, 508, 659
BshNI GGYRCC 1 cut(s) 775
BsiHKAI GWGCWC 1 cut(s) 960
BsiSI CCGG 3 cut(s) 148, 494, 505
BslFI GGGAC 2 cut(s) 18, 661
BslI CCNNNNNNNGG 3 cut(s) 141, 923, 1189
BsmAI GTCTC 2 cut(s) 523, 805
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 2 cut(s) 18, 661
BsnI GGCC 7 cut(s) 179, 269, 278, 300, 404, 508, 659
Bsp119I TTCGAA 1 cut(s) 25
Bsp1286I GDGCHC 1 cut(s) 960
Bsp13I TCCGGA 1 cut(s) 147
Bsp1407I TGTACA 1 cut(s) 570
Bsp143I GATC 2 cut(s) 144, 259
BspACI CCGC 4 cut(s) 44, 248, 251, 744
BspANI GGCC 7 cut(s) 179, 269, 278, 300, 404, 508, 659
BspCNI CTCAG 4 cut(s) 294, 630, 1043, 1069
BspEI TCCGGA 1 cut(s) 147
BspHI TCATGA 3 cut(s) 262, 607, 1135
BspLI GGNNCC 4 cut(s) 146, 340, 473, 777
BspPI GGATC 2 cut(s) 139, 152
BspT104I TTCGAA 1 cut(s) 25
BspT107I GGYRCC 1 cut(s) 775
BsrFI RCCGGY 1 cut(s) 504
BsrGI TGTACA 1 cut(s) 570
BsrI ACTGG 3 cut(s) 193, 351, 936
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 2 cut(s) 144, 259
BssNAI GTATAC 1 cut(s) 904
Bst1107I GTATAC 1 cut(s) 904
Bst4CI ACNGT 2 cut(s) 155, 235
Bst6I CTCTTC 2 cut(s) 116, 526
BstAPI GCANNNNNTGC 1 cut(s) 169
BstAUI TGTACA 1 cut(s) 570
BstBI TTCGAA 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 506, 702
BstDEI CTNAG 6 cut(s) 302, 638, 981, 1030, 1037, 1077
BstEII GGTNACC 1 cut(s) 128
BstF5I GGATG 2 cut(s) 291, 1047
BstKTI GATC 2 cut(s) 147, 262
BstMAI GTCTC 2 cut(s) 523, 805
BstMBI GATC 2 cut(s) 144, 259
BstMWI GCNNNNNNNGC 5 cut(s) 50, 169, 275, 510, 856
BstPAI GACNNNNGTC 1 cut(s) 919
BstPI GGTNACC 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 492
BstSLI GKGCMC 1 cut(s) 960
BstV1I GCAGC 1 cut(s) 980
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 144
BstXI CCANNNNNNTGG 2 cut(s) 200, 717
BstYI RGATCY 1 cut(s) 144
BstZ17I GTATAC 1 cut(s) 904
Bsu36I CCTNAGG 1 cut(s) 302
BsuRI GGCC 7 cut(s) 179, 269, 278, 300, 404, 508, 659
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 2 cut(s) 291, 1047
BtsIMutI CAGTG 1 cut(s) 591
Cac8I GCNNGC 2 cut(s) 506, 702
CciI TCATGA 3 cut(s) 262, 607, 1135
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 4 cut(s) 89, 471, 507, 914
Csp6I GTAC 3 cut(s) 571, 972, 1034
CviAII CATG 9 cut(s) 263, 400, 608, 856, 892, 908, 964, 1102, 1136
CviQI GTAC 3 cut(s) 571, 972, 1034
DdeI CTNAG 6 cut(s) 302, 638, 981, 1030, 1037, 1077
DpnI GATC 2 cut(s) 146, 261
DpnII GATC 2 cut(s) 144, 259
DraI TTTAAA 1 cut(s) 841
DraIII CACNNNGTG 1 cut(s) 955
EaeI YGGCCR 3 cut(s) 177, 276, 402
Eam1104I CTCTTC 2 cut(s) 116, 526
EarI CTCTTC 2 cut(s) 116, 526
Eco47I GGWCC 3 cut(s) 89, 471, 914
Eco57I CTGAAG 1 cut(s) 411
Eco81I CCTNAGG 1 cut(s) 302
Eco91I GGTNACC 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 471
EcoO65I GGTNACC 1 cut(s) 128
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 9 cut(s) 266, 403, 611, 859, 895, 911, 967, 1105, 1139
FaqI GGGAC 2 cut(s) 18, 661
FatI CATG 9 cut(s) 262, 399, 607, 855, 891, 907, 963, 1101, 1135
FauNDI CATATG 1 cut(s) 137
FblI GTMKAC 1 cut(s) 903
Fnu4HI GCNGC 3 cut(s) 248, 251, 994
FokI GGATG 2 cut(s) 278, 1034
Fsp4HI GCNGC 3 cut(s) 248, 251, 994
FspBI CTAG 4 cut(s) 54, 255, 767, 969
GluI GCNGC 3 cut(s) 248, 251, 994
GsuI CTGGAG 1 cut(s) 829
HaeIII GGCC 7 cut(s) 179, 269, 278, 300, 404, 508, 659
HapII CCGG 3 cut(s) 148, 494, 505
Hin1II CATG 9 cut(s) 266, 403, 611, 859, 895, 911, 967, 1105, 1139
HincII GTYRAC 1 cut(s) 1180
HindII GTYRAC 1 cut(s) 1180
HindIII AAGCTT 1 cut(s) 423
HinfI GANTC 3 cut(s) 204, 689, 1028
HpaII CCGG 3 cut(s) 148, 494, 505
HphI GGTGA 5 cut(s) 50, 140, 241, 758, 946
Hpy166II GTNNAC 4 cut(s) 490, 904, 958, 1180
Hpy188I TCNGA 2 cut(s) 159, 1078
Hpy188III TCNNGA 6 cut(s) 148, 263, 342, 469, 608, 1136
Hpy8I GTNNAC 4 cut(s) 490, 904, 958, 1180
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 4 cut(s) 766, 861, 905, 1142
HpyCH4III ACNGT 2 cut(s) 155, 235
HpyCH4IV ACGT 3 cut(s) 384, 516, 528
HpyCH4V TGCA 6 cut(s) 163, 479, 800, 958, 996, 1092
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 169, 275, 510, 856
HpyF3I CTNAG 6 cut(s) 302, 638, 981, 1030, 1037, 1077
HpySE526I ACGT 3 cut(s) 384, 516, 528
Hsp92II CATG 9 cut(s) 266, 403, 611, 859, 895, 911, 967, 1105, 1139
Kpn2I TCCGGA 1 cut(s) 147
KroI GCCGGC 1 cut(s) 504
KroNI GCCGGC 1 cut(s) 506
Kzo9I GATC 2 cut(s) 144, 259
LmnI GCTCC 1 cut(s) 97
Lsp1109I GCAGC 1 cut(s) 980
LweI GCATC 3 cut(s) 68, 150, 159
MaeI CTAG 4 cut(s) 54, 255, 767, 969
MaeII ACGT 3 cut(s) 384, 516, 528
MaeIII GTNAC 3 cut(s) 128, 229, 1006
MalI GATC 2 cut(s) 146, 261
MboI GATC 2 cut(s) 144, 259
MboII GAAGA 6 cut(s) 74, 87, 103, 513, 566, 1117
MflI RGATCY 1 cut(s) 144
MhlI GDGCHC 1 cut(s) 960
MlsI TGGCCA 2 cut(s) 278, 404
MluCI AATT 7 cut(s) 21, 480, 682, 758, 842, 940, 1139
MluNI TGGCCA 2 cut(s) 278, 404
MlyI GAGTC 2 cut(s) 213, 1022
MmeI TCCRAC 3 cut(s) 165, 181, 549
Mox20I TGGCCA 2 cut(s) 278, 404
MroI TCCGGA 1 cut(s) 147
MroNI GCCGGC 1 cut(s) 504
MscI TGGCCA 2 cut(s) 278, 404
MseI TTAA 1 cut(s) 840
Msp20I TGGCCA 2 cut(s) 278, 404
MspA1I CMGCKG 1 cut(s) 744
MspI CCGG 3 cut(s) 148, 494, 505
MspR9I CCNGG 1 cut(s) 494
MwoI GCNNNNNNNGC 5 cut(s) 50, 169, 275, 510, 856
NaeI GCCGGC 1 cut(s) 506
NciI CCSGG 1 cut(s) 494
NdeI CATATG 1 cut(s) 137
NdeII GATC 2 cut(s) 144, 259
NgoMIV GCCGGC 1 cut(s) 504
NlaIII CATG 9 cut(s) 266, 403, 611, 859, 895, 911, 967, 1105, 1139
NlaIV GGNNCC 4 cut(s) 146, 340, 473, 777
NmeAIII GCCGAG 3 cut(s) 205, 276, 586
NmuCI GTSAC 2 cut(s) 128, 229
NspV TTCGAA 1 cut(s) 25
PagI TCATGA 3 cut(s) 262, 607, 1135
PcsI WCGNNNNNNNCGW 1 cut(s) 378
PdiI GCCGGC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 689
PkrI GCNGC 3 cut(s) 249, 252, 995
PleI GAGTC 2 cut(s) 212, 1022
PpsI GAGTC 2 cut(s) 212, 1022
PpuMI RGGWCCY 1 cut(s) 471
PshAI GACNNNNGTC 1 cut(s) 919
Psp1406I AACGTT 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 471
PspEI GGTNACC 1 cut(s) 128
PspN4I GGNNCC 4 cut(s) 146, 340, 473, 777
PspPI GGNCC 4 cut(s) 89, 471, 507, 914
PspPPI RGGWCCY 1 cut(s) 471
PsrI GAACNNNNNNTAC 2 cut(s) 480, 512
PsuI RGATCY 1 cut(s) 144
RsaI GTAC 3 cut(s) 572, 973, 1035
RsaNI GTAC 3 cut(s) 571, 972, 1034
SaqAI TTAA 1 cut(s) 840
SatI GCNGC 3 cut(s) 248, 251, 994
Sau3AI GATC 2 cut(s) 144, 259
Sau96I GGNCC 4 cut(s) 89, 471, 507, 914
ScaI AGTACT 2 cut(s) 973, 1035
SchI GAGTC 2 cut(s) 213, 1022
ScrFI CCNGG 1 cut(s) 494
SduI GDGCHC 1 cut(s) 960
SfaNI GCATC 3 cut(s) 68, 150, 159
SfiI GGCCNNNNNGGCC 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 25
SinI GGWCC 3 cut(s) 89, 471, 914
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 7 cut(s) 21, 480, 682, 758, 842, 940, 1139
SsiI CCGC 4 cut(s) 44, 248, 251, 744
SspMI CTAG 4 cut(s) 54, 255, 767, 969
StyD4I CCNGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 155, 235
TaiI ACGT 3 cut(s) 387, 519, 531
TaqI TCGA 2 cut(s) 25, 218
TasI AATT 7 cut(s) 21, 480, 682, 758, 842, 940, 1139
TatI WGTACW 3 cut(s) 570, 971, 1033
TauI GCSGC 2 cut(s) 250, 253
TfiI GAWTC 1 cut(s) 689
Tru1I TTAA 1 cut(s) 840
Tru9I TTAA 1 cut(s) 840
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 128, 229
TseI GCWGC 1 cut(s) 993
Tsp45I GTSAC 2 cut(s) 128, 229
TspDTI ATGAA 8 cut(s) 227, 430, 880, 924, 1118, 1124, 1128, 1152
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 1 cut(s) 598
VneI GTGCAC 1 cut(s) 956
VpaK11BI GGWCC 3 cut(s) 89, 471, 914
XapI RAATTY 1 cut(s) 940
XmiI GTMKAC 1 cut(s) 903
XspI CTAG 4 cut(s) 54, 255, 767, 969
ZrmI AGTACT 2 cut(s) 973, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.