Rw6G002110

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000427
Physical Location & Seq
Reverse (-)
3307223 .. 3310746
3524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002110.1

Sequence Viewer

Length: 1611 bp
ATGGCTACCAATTCGTGTTCTCTCTTCTGGGTTCTCCTTCTAATTCTTCAAAGCACACTATCAAACGGGTCTGAATCCATTACACCCAGAAGCCTAATGGAAGAAGCCACAGTTGATGATCTCCAACTCGCTTTCAAGCAAAAGCTTCTGACTTCAAGGCAAGTCGTTCAGTTCTACCTGAACCAAATCCACAAACTCAACCAAGACCTCAGAGGAGTCTTAGAGATAAACCCAGATGCTTTGTCGCAAGCTGACATAGCAGACAAAGAGCGCAGTGAATATGAATCCAGTGCCCAAACACTGCCTAAGCTTCATGGGATTCCTGTTCTGCTCAAGGATAATATAGCAACCAAGGACAAGCTTAACACCACTGCTGGGTCTTATGCTCTTTTGGGTTCCGTAGTGCCTAGAGATGCTGGGGTTGTCTCAAAGTTGAGGAGCTCTGGGGCTATCATTTTGGGGAAGGCTACGTTGAGTGAATGGGCCTATATCAGAACATCTAAAGCACCCTATGGTTGGAGTGCCAGAGGTGGTCAAGGAGTGAATCCTTATAATTCATCACTGGAAGTTTGTGGATCAAGTAGTGGATCCGCAATATCAGTATCTGCAAATATGGTATCAGTGTCTCTAGGAACAGAGACCGATAGCTCCATCTTATGTCCAGCGAGTTTTAACTCGGTAGTGGGCTTCAAACCAACGGTTGGTCTCACTAGTCGAGCAGGAGTCATCCCAGTCAGTCCAAGACAGGACACAATTGGGCCAATTTGTAGGACTGTAGCGGATGCTGTTCATGTCCTTGATACCATTGTTGGCATTGATAGCAATGACAAGGCAACAAAAGAAGTATCCCAGTATATTCCAGTGGGTGGCTATAGCCAGTTTCTCAATTCTAATGGTCTCAAAGGAAAGAGACTGGGGATAGTCAGTCTCATGTTTCTTCAGATCAAGGATGATGCTTTCTTGAATCAAACTTTTGAGCAGCATTTTACCACACTGAGGGAACAAGGTGCAATTTTGGTTGAGAATCTGGAAATAGCCAACCTTGAAGATATTCGCGATTTTACTTCAAGTGGTGAGTTTGTAGCAATGTTAGCTGAATTCAAGAGCGCCTTGAATGTGTACCTGGGTGACCTAGTCAAATCTCCAGTGAGAACCTTAGGCCAGGTTATAGCATTCAACAAGAATAATTCTTATGTGGAAAAGAATGATGATTATGGACAAAATCTTTTAGAAGCAGCTGAGGAGACTAATGGAATGGGAGCAAAAGAGCTTGAGGCATTGTCAAATATAGAGAGACTATCTAGAGATGGATTTGAGAAGTTGATGACAGATAATAACTTAGATGCATTGGTGACTTATGCTAATAGCGCTTCTTCTATCCTCGCAATTGGTGGTTTCCCAGGCATCGTTGTGCCAGCAGGATATCATGACACTGATAAGTACCCTTTTGGAATTTGCTTTGGGGGATTGAAGGGTTCGGAGCCAAAGCTGATCGAGATTGCCTATGCCTTTGAGCAAGCAACTATGATCAGGAAGCCTCCAACTGTTGATTCTCAATACCAAAGTATCTCTACCACCGTCACAAGAGGCTCCAACGTAACTACTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

536

Amino Acids

57.77

Weight (kDa)

5.17

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 55 - 452 1.3e-70 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AccB7I CCANNNNNTGG 2 cut(s) 701, 866
AccII CGCG 1 cut(s) 1056
AciI CCGC 2 cut(s) 591, 779
AclWI GGATC 3 cut(s) 582, 583, 595
AcsI RAATTY 2 cut(s) 1097, 1452
AcuI CTGAAG 1 cut(s) 923
AfaI GTAC 2 cut(s) 1121, 1442
AfeI AGCGCT 1 cut(s) 1369
AfiI CCNNNNNNNGG 5 cut(s) 375, 516, 701, 866, 996
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 3 cut(s) 1122, 1161, 1399
AjuI GAANNNNNNNTTGG 2 cut(s) 1534, 1566
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 9 cut(s) 430, 630, 632, 710, 902, 904, 932, 1238, 1288
AlwI GGATC 3 cut(s) 582, 583, 595
AlwNI CAGNNNCTG 1 cut(s) 605
Aor51HI AGCGCT 1 cut(s) 1369
AoxI GGCC 3 cut(s) 483, 758, 1159
ApeKI GCWGC 2 cut(s) 979, 1235
ApoI RAATTY 2 cut(s) 1097, 1452
Asp700I GAANNNNTTC 1 cut(s) 1050
AspLEI GCGC 3 cut(s) 273, 1109, 1370
AspS9I GGNCC 2 cut(s) 483, 758
AsuHPI GGTGA 3 cut(s) 1085, 1139, 1363
AxyI CCTNAGG 1 cut(s) 1156
BaeGI GKGCMC 1 cut(s) 295
BaeI ACNNNNGTAYC 4 cut(s) 792, 825, 1549, 1582
BamHI GGATCC 1 cut(s) 587
BanII GRGCYC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 443
BbvCI CCTCAGC 1 cut(s) 1239
BbvI GCAGC 2 cut(s) 991, 1247
BccI CCATC 2 cut(s) 659, 1301
BciT130I CCWGG 3 cut(s) 1124, 1163, 1401
BciVI GTATCC 1 cut(s) 856
BclI TGATCA 1 cut(s) 1527
BcoDI GTCTC 9 cut(s) 430, 630, 632, 710, 902, 904, 932, 1238, 1288
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 5 cut(s) 408, 629, 711, 1133, 1302
BfmI CTRYAG 2 cut(s) 774, 871
BfoI RGCGCY 2 cut(s) 1110, 1371
BfuI GTATCC 1 cut(s) 856
BisI GCNGC 2 cut(s) 980, 1236
BlsI GCNGC 2 cut(s) 981, 1237
Bme1390I CCNGG 3 cut(s) 1124, 1163, 1401
BmgT120I GGNCC 2 cut(s) 483, 758
BmiI GGNNCC 4 cut(s) 397, 589, 1482, 1591
BmrFI CCNGG 3 cut(s) 1124, 1163, 1401
BmrI ACTGGG 3 cut(s) 725, 844, 923
BmsI GCATC 6 cut(s) 226, 403, 772, 943, 1333, 1413
BmuI ACTGGG 3 cut(s) 725, 844, 923
BpmI CTGGAG 1 cut(s) 1128
Bpu10I CCTNAGC 2 cut(s) 306, 1239
BpuEI CTTGAG 2 cut(s) 317, 1292
BsaI GGTCTC 3 cut(s) 632, 710, 902
BsaJI CCNNGG 3 cut(s) 351, 1123, 1399
Bsc4I CCNNNNNNNGG 5 cut(s) 375, 516, 701, 866, 996
Bse1I ACTGG 8 cut(s) 288, 567, 731, 850, 860, 877, 918, 1145
Bse21I CCTNAGG 1 cut(s) 1156
Bse3DI GCAATG 2 cut(s) 829, 1092
BseBI CCWGG 3 cut(s) 1124, 1163, 1401
BseDI CCNNGG 3 cut(s) 351, 1123, 1399
BseGI GGATG 3 cut(s) 726, 787, 955
BseLI CCNNNNNNNGG 5 cut(s) 375, 516, 701, 866, 996
BseMI GCAATG 2 cut(s) 829, 1092
BseMII CTCAG 3 cut(s) 223, 986, 1230
BseNI ACTGG 8 cut(s) 288, 567, 731, 850, 860, 877, 918, 1145
BseRI GAGGAG 3 cut(s) 228, 451, 1256
BseSI GKGCMC 1 cut(s) 295
BseXI GCAGC 2 cut(s) 991, 1247
BseYI CCCAGC 2 cut(s) 374, 416
Bsh1236I CGCG 1 cut(s) 1056
BshFI GGCC 3 cut(s) 485, 760, 1161
BsiHKAI GWGCWC 1 cut(s) 443
BslI CCNNNNNNNGG 5 cut(s) 375, 516, 701, 866, 996
BsmAI GTCTC 9 cut(s) 430, 630, 632, 710, 902, 904, 932, 1238, 1288
BsmI GAATGC 1 cut(s) 1172
BsnI GGCC 3 cut(s) 485, 760, 1161
Bso31I GGTCTC 3 cut(s) 632, 710, 902
Bsp1286I GDGCHC 2 cut(s) 295, 443
Bsp143I GATC 6 cut(s) 118, 575, 587, 942, 1491, 1527
Bsp68I TCGCGA 1 cut(s) 1056
BspACI CCGC 2 cut(s) 591, 779
BspANI GGCC 3 cut(s) 485, 760, 1161
BspCNI CTCAG 3 cut(s) 222, 987, 1231
BspFNI CGCG 1 cut(s) 1056
BspHI TCATGA 1 cut(s) 1426
BspLI GGNNCC 4 cut(s) 397, 589, 1482, 1591
BspPI GGATC 3 cut(s) 582, 583, 595
BspTNI GGTCTC 3 cut(s) 632, 710, 902
BsrDI GCAATG 2 cut(s) 829, 1092
BsrI ACTGG 8 cut(s) 288, 567, 731, 850, 860, 877, 918, 1145
BssECI CCNNGG 3 cut(s) 351, 1123, 1399
BssMI GATC 6 cut(s) 118, 575, 587, 942, 1491, 1527
BssT1I CCWWGG 1 cut(s) 351
Bst2UI CCWGG 3 cut(s) 1124, 1163, 1401
Bst4CI ACNGT 5 cut(s) 112, 700, 775, 1546, 1579
Bst6I CTCTTC 1 cut(s) 29
BstC8I GCNNGC 3 cut(s) 249, 1416, 1518
BstDEI CTNAG 7 cut(s) 209, 220, 306, 995, 1156, 1239, 1339
BstEII GGTNACC 1 cut(s) 1127
BstF5I GGATG 3 cut(s) 726, 787, 955
BstFNI CGCG 1 cut(s) 1056
BstH2I RGCGCY 2 cut(s) 1110, 1371
BstHHI GCGC 3 cut(s) 273, 1109, 1370
BstKTI GATC 6 cut(s) 121, 578, 590, 945, 1494, 1530
BstMAI GTCTC 9 cut(s) 430, 630, 632, 710, 902, 904, 932, 1238, 1288
BstMBI GATC 6 cut(s) 118, 575, 587, 942, 1491, 1527
BstMWI GCNNNNNNNGC 4 cut(s) 257, 819, 1091, 1367
BstNI CCWGG 3 cut(s) 1124, 1163, 1401
BstPI GGTNACC 1 cut(s) 1127
BstSCI CCNGG 3 cut(s) 1122, 1161, 1399
BstSFI CTRYAG 2 cut(s) 774, 871
BstSLI GKGCMC 1 cut(s) 295
BstUI CGCG 1 cut(s) 1056
BstV1I GCAGC 2 cut(s) 991, 1247
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
Bsu36I CCTNAGG 1 cut(s) 1156
BsuI GTATCC 1 cut(s) 856
BsuRI GGCC 3 cut(s) 485, 760, 1161
BtsCI GGATG 3 cut(s) 726, 787, 955
BtsI GCAGTG 3 cut(s) 280, 299, 369
BtuMI TCGCGA 1 cut(s) 1056
Cac8I GCNNGC 3 cut(s) 249, 1416, 1518
CaiI CAGNNNCTG 1 cut(s) 605
CciI TCATGA 1 cut(s) 1426
CfoI GCGC 3 cut(s) 273, 1109, 1370
Cfr13I GGNCC 2 cut(s) 483, 758
Csp6I GTAC 2 cut(s) 1120, 1441
CviAII CATG 4 cut(s) 314, 791, 931, 1427
CviQI GTAC 2 cut(s) 1120, 1441
DdeI CTNAG 7 cut(s) 209, 220, 306, 995, 1156, 1239, 1339
DpnI GATC 6 cut(s) 120, 577, 589, 944, 1493, 1529
DpnII GATC 6 cut(s) 118, 575, 587, 942, 1491, 1527
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 443
Eco31I GGTCTC 3 cut(s) 632, 710, 902
Eco32I GATATC 1 cut(s) 1424
Eco47III AGCGCT 1 cut(s) 1369
Eco53kI GAGCTC 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 923
Eco81I CCTNAGG 1 cut(s) 1156
Eco91I GGTNACC 1 cut(s) 1127
EcoICRI GAGCTC 1 cut(s) 441
EcoO65I GGTNACC 1 cut(s) 1127
EcoRI GAATTC 1 cut(s) 1097
EcoRII CCWGG 3 cut(s) 1122, 1161, 1399
EcoRV GATATC 1 cut(s) 1424
EcoT14I CCWWGG 1 cut(s) 351
EcoT22I ATGCAT 1 cut(s) 1348
EcoT38I GRGCYC 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 4 cut(s) 317, 794, 934, 1430
FalI AAGNNNNNCTT 2 cut(s) 1094, 1126
FatI CATG 4 cut(s) 313, 790, 930, 1426
FbaI TGATCA 1 cut(s) 1527
Fnu4HI GCNGC 2 cut(s) 980, 1236
FokI GGATG 3 cut(s) 713, 794, 962
FriOI GRGCYC 1 cut(s) 443
Fsp4HI GCNGC 2 cut(s) 980, 1236
FspBI CTAG 5 cut(s) 408, 629, 711, 1133, 1302
GlaI GCGC 3 cut(s) 272, 1108, 1369
GluI GCNGC 2 cut(s) 980, 1236
GsaI CCCAGC 2 cut(s) 378, 420
GsuI CTGGAG 1 cut(s) 1128
HaeII RGCGCY 2 cut(s) 1110, 1371
HaeIII GGCC 3 cut(s) 485, 760, 1161
HhaI GCGC 3 cut(s) 273, 1109, 1370
Hin1II CATG 4 cut(s) 317, 794, 934, 1430
Hin6I GCGC 3 cut(s) 271, 1107, 1368
HinP1I GCGC 3 cut(s) 271, 1107, 1368
HindIII AAGCTT 3 cut(s) 143, 308, 359
HinfI GANTC 9 cut(s) 74, 216, 284, 319, 544, 723, 964, 1024, 1550
HphI GGTGA 3 cut(s) 1085, 1139, 1363
Hpy166II GTNNAC 1 cut(s) 1120
Hpy188I TCNGA 6 cut(s) 73, 150, 212, 494, 942, 1480
Hpy188III TCNNGA 8 cut(s) 961, 1028, 1055, 1102, 1302, 1427, 1495, 1531
Hpy8I GTNNAC 1 cut(s) 1120
HpyAV CCTTC 3 cut(s) 47, 457, 1465
HpyCH4III ACNGT 5 cut(s) 112, 700, 775, 1546, 1579
HpyCH4IV ACGT 2 cut(s) 470, 1596
HpyCH4V TGCA 3 cut(s) 608, 1010, 1346
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 819, 1091, 1367
HpyF3I CTNAG 7 cut(s) 209, 220, 306, 995, 1156, 1239, 1339
HpySE526I ACGT 2 cut(s) 470, 1596
Hsp92II CATG 4 cut(s) 317, 794, 934, 1430
HspAI GCGC 3 cut(s) 271, 1107, 1368
Ksp22I TGATCA 1 cut(s) 1527
Kzo9I GATC 6 cut(s) 118, 575, 587, 942, 1491, 1527
LmnI GCTCC 5 cut(s) 438, 653, 1259, 1480, 1595
Lsp1109I GCAGC 2 cut(s) 991, 1247
LweI GCATC 6 cut(s) 226, 403, 772, 943, 1333, 1413
MaeI CTAG 5 cut(s) 408, 629, 711, 1133, 1302
MaeII ACGT 2 cut(s) 470, 1596
MaeIII GTNAC 4 cut(s) 1127, 1351, 1579, 1597
MalI GATC 6 cut(s) 120, 577, 589, 944, 1493, 1529
MboI GATC 6 cut(s) 118, 575, 587, 942, 1491, 1527
MboII GAAGA 6 cut(s) 16, 38, 113, 929, 1058, 1365
MfeI CAATTG 2 cut(s) 753, 1386
MflI RGATCY 1 cut(s) 587
MhlI GDGCHC 2 cut(s) 295, 443
MlyI GAGTC 2 cut(s) 225, 732
MmeI TCCRAC 3 cut(s) 148, 497, 1565
Mph1103I ATGCAT 1 cut(s) 1348
MroXI GAANNNNTTC 1 cut(s) 1050
MseI TTAA 2 cut(s) 363, 672
MslI CAYNNNNRTG 1 cut(s) 1409
MspA1I CMGCKG 1 cut(s) 1238
MspR9I CCNGG 3 cut(s) 1124, 1163, 1401
MunI CAATTG 2 cut(s) 753, 1386
Mva1269I GAATGC 1 cut(s) 1172
MvaI CCWGG 3 cut(s) 1124, 1163, 1401
MvnI CGCG 1 cut(s) 1056
MwoI GCNNNNNNNGC 4 cut(s) 257, 819, 1091, 1367
NdeII GATC 6 cut(s) 118, 575, 587, 942, 1491, 1527
NlaIII CATG 4 cut(s) 317, 794, 934, 1430
NlaIV GGNNCC 4 cut(s) 397, 589, 1482, 1591
NmuCI GTSAC 3 cut(s) 1127, 1351, 1579
NruI TCGCGA 1 cut(s) 1056
NsiI ATGCAT 1 cut(s) 1348
PagI TCATGA 1 cut(s) 1426
PctI GAATGC 1 cut(s) 1172
PdmI GAANNNNTTC 1 cut(s) 1050
PfeI GAWTC 7 cut(s) 74, 284, 319, 544, 964, 1024, 1550
PflFI GACNNNGTC 1 cut(s) 1133
PflMI CCANNNNNTGG 2 cut(s) 701, 866
PkrI GCNGC 2 cut(s) 981, 1237
PleI GAGTC 2 cut(s) 224, 731
PpsI GAGTC 2 cut(s) 224, 731
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 443
Psp6I CCWGG 3 cut(s) 1122, 1161, 1399
PspEI GGTNACC 1 cut(s) 1127
PspFI CCCAGC 2 cut(s) 374, 416
PspGI CCWGG 3 cut(s) 1122, 1161, 1399
PspN4I GGNNCC 4 cut(s) 397, 589, 1482, 1591
PspPI GGNCC 2 cut(s) 483, 758
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 1 cut(s) 587
PsyI GACNNNGTC 1 cut(s) 1133
PvuII CAGCTG 1 cut(s) 1238
RruI TCGCGA 1 cut(s) 1056
RsaI GTAC 2 cut(s) 1121, 1442
RsaNI GTAC 2 cut(s) 1120, 1441
RseI CAYNNNNRTG 1 cut(s) 1409
SacI GAGCTC 1 cut(s) 443
SaqAI TTAA 2 cut(s) 363, 672
SatI GCNGC 2 cut(s) 980, 1236
Sau3AI GATC 6 cut(s) 118, 575, 587, 942, 1491, 1527
Sau96I GGNCC 2 cut(s) 483, 758
SchI GAGTC 2 cut(s) 225, 732
ScrFI CCNGG 3 cut(s) 1124, 1163, 1401
SduI GDGCHC 2 cut(s) 295, 443
SfaNI GCATC 6 cut(s) 226, 403, 772, 943, 1333, 1413
SfcI CTRYAG 2 cut(s) 774, 871
SmiMI CAYNNNNRTG 1 cut(s) 1409
SmlI CTYRAG 2 cut(s) 332, 1271
SmoI CTYRAG 2 cut(s) 332, 1271
SpeI ACTAGT 1 cut(s) 710
SsiI CCGC 2 cut(s) 591, 779
SspMI CTAG 5 cut(s) 408, 629, 711, 1133, 1302
SstI GAGCTC 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 1122, 1161, 1399
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 5 cut(s) 112, 700, 775, 1546, 1579
TaiI ACGT 2 cut(s) 473, 1599
TaqI TCGA 2 cut(s) 715, 1494
TaqII GACCGA 1 cut(s) 656
TfiI GAWTC 7 cut(s) 74, 284, 319, 544, 964, 1024, 1550
Tru1I TTAA 2 cut(s) 363, 672
Tru9I TTAA 2 cut(s) 363, 672
TseFI GTSAC 3 cut(s) 1127, 1351, 1579
TseI GCWGC 2 cut(s) 979, 1235
Tsp45I GTSAC 3 cut(s) 1127, 1351, 1579
TspDTI ATGAA 4 cut(s) 297, 302, 546, 779
TspGWI ACGGA 1 cut(s) 388
Tth111I GACNNNGTC 1 cut(s) 1133
Van91I CCANNNNNTGG 2 cut(s) 701, 866
XapI RAATTY 2 cut(s) 1097, 1452
XbaI TCTAGA 1 cut(s) 1301
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmnI GAANNNNTTC 1 cut(s) 1050
XspI CTAG 5 cut(s) 408, 629, 711, 1133, 1302
Zsp2I ATGCAT 1 cut(s) 1348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.