FvH4_2g01722

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000427
Physical Location & Seq
Reverse (-)
1519307 .. 1521900
2594 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01722.t1

Sequence Viewer

Length: 1575 bp
ATGATAATTTCCTCATCCACTGCCATGGCCAAACCAAGCTTCCCTCACATCTCATCCTTGTTGCTTCTCATTCTTCTCCTCACATTGTCACCTCTGCCAGGCACATTAGCAGCCGGCAAACTGTCGCTGAAAGAAGCCACCATAGATGATATCCAGCACGCTTTCAAGCACAACCAACTCACCTCAAGGCAGCTTGTCCAATATTACCTTGTCCAAATCCGGAAACTGAATCCGGTCCTCAAAGGAGTCCTAGAAGTCAATCCGGATGCGCTTCACCTTGCTGACAAGGCTGACTATGAGCGCAAGACCAAGGCACCAGTGTCACTACTCTCCAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACCACCGCCGGCTCTTTGGCACTGCTTGGATCTGTTGTGCCTCGTGATGCCAGCGTGGTGGAGAAGTTGAGGAAAGCTGGCGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGGGTGCTGCACCCTCTGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGTGGTTCAGCAATATCAGTTGCAGCAAATTTGGCAATGGTGTCATTAGGTACCGAGACAGATGGTTCAATCTTATGTCCATCAGGTTATAACTCAGTTGTGGGGATCAAACCAACAATTGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCAAAGACAGGATACTGTTGGGCCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATTGACCACAATGACATTGCAACAATTGAGACATCAAAGTACATCCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCACCATTTTGAGGAAAGCAGGTGCTGTTTTGGTAGACAATTTGGAAGTGGCTAATTGGACTGCAATCTATTCTTCAAGCGATGAAATTAATGCATTGTCTGTTGAGTGCAAAATATCTTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCGGTGCGATCATTGGCAGATGTTATAGCCTTCAACAACAAACACCAGAAACTGGAAAAGATCAAGGAGTACGGGCAAGAACTATTTTTAGCAGCAGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGGCAGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTGAGATTGGTGACAGAGAAGAAGCTAGATGCTGTGGTGACTCCTAAAGCAGTTTTCTCAAGTGTACTTGCAATTGGGGGTGCCCCAGGTCTAATAGTTCCAGCCGGGTACGAAAACAATGGGAGACCAGCTGGTATATGCTTTGGAGGACTTCGGGGTTCAGAGCCAAAGCTAATTGAGATTGCATACGCTTTTGAGCAAGCCACTAAGATTAGGAAGCCTCCTTCCTTCAATGGCCTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

525

Amino Acids

56.0

Weight (kDa)

9.44

Isoelectric Point (pI)

31.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 65 - 458 3.1e-70 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AarI CACCTGC 1 cut(s) 1013
Acc36I ACCTGC 1 cut(s) 1013
Acc65I GGTACC 1 cut(s) 653
AccB1I GGYRCC 3 cut(s) 313, 653, 1406
AccB7I CCANNNNNTGG 2 cut(s) 722, 1207
AccI GTMKAC 1 cut(s) 1038
AccII CGCG 1 cut(s) 912
AccIII TCCGGA 2 cut(s) 219, 262
AciI CCGC 3 cut(s) 399, 555, 594
AclWI GGATC 2 cut(s) 430, 716
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 631
AfaI GTAC 5 cut(s) 655, 875, 1226, 1392, 1436
AfiI CCNNNNNNNGG 6 cut(s) 98, 540, 722, 887, 1014, 1207
AgsI TTSAA 8 cut(s) 166, 568, 672, 1080, 1138, 1189, 1297, 1558
AhlI ACTAGT 1 cut(s) 731
AjnI CCWGG 2 cut(s) 97, 1411
AjuI GAANNNNNNNTTGG 2 cut(s) 23, 55
AluBI AGCT 7 cut(s) 39, 193, 470, 498, 1351, 1457, 1498
AluI AGCT 7 cut(s) 39, 193, 470, 498, 1351, 1457, 1498
Alw26I GTCTC 3 cut(s) 653, 857, 1444
AlwI GGATC 2 cut(s) 430, 716
AlwNI CAGNNNCTG 3 cut(s) 806, 1028, 1207
Aor13HI TCCGGA 2 cut(s) 219, 262
AoxI GGCC 3 cut(s) 27, 779, 1561
ApeKI GCWGC 7 cut(s) 110, 190, 527, 626, 806, 1247, 1286
ApoI RAATTY 1 cut(s) 631
AseI ATTAAT 1 cut(s) 1092
Asp718I GGTACC 1 cut(s) 653
AspLEI GCGC 5 cut(s) 271, 303, 476, 548, 912
AspS9I GGNCC 2 cut(s) 235, 779
AsuC2I CCSGG 2 cut(s) 1157, 1432
AsuHPI GGTGA 6 cut(s) 81, 172, 266, 997, 1348, 1375
AsuNHI GCTAGC 1 cut(s) 494
AvaII GGWCC 1 cut(s) 235
BaeGI GKGCMC 1 cut(s) 1411
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 3 cut(s) 313, 653, 1406
BauI CACGAG 1 cut(s) 435
BbvI GCAGC 7 cut(s) 122, 202, 514, 638, 793, 1259, 1298
BccI CCATC 2 cut(s) 659, 691
BciT130I CCWGG 2 cut(s) 99, 1413
BciVI GTATCC 1 cut(s) 763
BcnI CCSGG 2 cut(s) 1157, 1432
BcoDI GTCTC 3 cut(s) 653, 857, 1444
BcuI ACTAGT 1 cut(s) 731
BfaI CTAG 4 cut(s) 251, 495, 732, 1352
BfoI RGCGCY 2 cut(s) 477, 549
BfuAI ACCTGC 1 cut(s) 1013
BfuI GTATCC 1 cut(s) 763
BisI GCNGC 8 cut(s) 111, 191, 528, 595, 627, 807, 1248, 1287
BlsI GCNGC 8 cut(s) 112, 192, 529, 596, 628, 808, 1249, 1288
Bme1390I CCNGG 4 cut(s) 99, 1157, 1413, 1432
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 235, 779
BmiI GGNNCC 3 cut(s) 315, 655, 1408
BmrFI CCNGG 4 cut(s) 99, 1157, 1413, 1432
BmsI GCATC 5 cut(s) 256, 430, 793, 811, 1345
BmtI GCTAGC 1 cut(s) 498
BpuEI CTTGAG 5 cut(s) 169, 520, 583, 1369, 1550
BpuMI CCSGG 2 cut(s) 1157, 1432
BsaI GGTCTC 1 cut(s) 1444
BsaJI CCNNGG 6 cut(s) 24, 309, 378, 978, 1155, 1411
BsaWI WCCGGW 3 cut(s) 219, 232, 262
Bsc4I CCNNNNNNNGG 6 cut(s) 98, 540, 722, 887, 1014, 1207
Bse118I RCCGGY 2 cut(s) 113, 401
Bse1I ACTGG 2 cut(s) 317, 1212
Bse3DI GCAATG 4 cut(s) 369, 645, 849, 973
BseAI TCCGGA 2 cut(s) 219, 262
BseBI CCWGG 2 cut(s) 99, 1413
BseDI CCNNGG 6 cut(s) 24, 309, 378, 978, 1155, 1411
BseGI GGATG 4 cut(s) 14, 53, 271, 876
BseLI CCNNNNNNNGG 6 cut(s) 98, 540, 722, 887, 1014, 1207
BseMI GCAATG 4 cut(s) 369, 645, 849, 973
BseMII CTCAG 3 cut(s) 597, 711, 919
BseNI ACTGG 2 cut(s) 317, 1212
BseRI GAGGAG 1 cut(s) 68
BseSI GKGCMC 1 cut(s) 1411
BseXI GCAGC 7 cut(s) 122, 202, 514, 638, 793, 1259, 1298
BsgI GTGCAG 1 cut(s) 513
Bsh1236I CGCG 1 cut(s) 912
BshFI GGCC 3 cut(s) 29, 781, 1563
BshNI GGYRCC 3 cut(s) 313, 653, 1406
BsiSI CCGG 7 cut(s) 114, 220, 233, 263, 402, 1157, 1431
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 6 cut(s) 98, 540, 722, 887, 1014, 1207
BsmAI GTCTC 3 cut(s) 653, 857, 1444
BsmFI GGGAC 1 cut(s) 734
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 3 cut(s) 29, 781, 1563
Bso31I GGTCTC 1 cut(s) 1444
Bsp1286I GDGCHC 1 cut(s) 1411
Bsp13I TCCGGA 2 cut(s) 219, 262
Bsp143I GATC 4 cut(s) 422, 708, 1163, 1215
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 3 cut(s) 399, 555, 594
BspANI GGCC 3 cut(s) 29, 781, 1563
BspCNI CTCAG 3 cut(s) 596, 710, 918
BspEI TCCGGA 2 cut(s) 219, 262
BspFNI CGCG 1 cut(s) 912
BspLI GGNNCC 3 cut(s) 315, 655, 1408
BspMI ACCTGC 1 cut(s) 1013
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 2 cut(s) 430, 716
BspT107I GGYRCC 3 cut(s) 313, 653, 1406
BspTNI GGTCTC 1 cut(s) 1444
BsrDI GCAATG 4 cut(s) 369, 645, 849, 973
BsrFI RCCGGY 2 cut(s) 113, 401
BsrI ACTGG 2 cut(s) 317, 1212
BssAI RCCGGY 2 cut(s) 113, 401
BssECI CCNNGG 6 cut(s) 24, 309, 378, 978, 1155, 1411
BssMI GATC 4 cut(s) 422, 708, 1163, 1215
BssSI CACGAG 1 cut(s) 435
BssT1I CCWWGG 4 cut(s) 24, 309, 378, 978
Bst2BI CACGAG 1 cut(s) 435
Bst2UI CCWGG 2 cut(s) 99, 1413
Bst4CI ACNGT 4 cut(s) 123, 775, 796, 900
BstAPI GCANNNNNTGC 1 cut(s) 806
BstC8I GCNNGC 8 cut(s) 115, 159, 403, 445, 472, 496, 832, 1527
BstDEI CTNAG 5 cut(s) 583, 697, 905, 920, 1533
BstDSI CCRYGG 1 cut(s) 24
BstENI CCTNNNNNAGG 1 cut(s) 96
BstF5I GGATG 4 cut(s) 14, 53, 271, 876
BstFNI CGCG 1 cut(s) 912
BstH2I RGCGCY 2 cut(s) 477, 549
BstHHI GCGC 5 cut(s) 271, 303, 476, 548, 912
BstKTI GATC 4 cut(s) 425, 711, 1166, 1218
BstMAI GTCTC 3 cut(s) 653, 857, 1444
BstMBI GATC 4 cut(s) 422, 708, 1163, 1215
BstMWI GCNNNNNNNGC 7 cut(s) 287, 552, 561, 600, 635, 806, 1253
BstNI CCWGG 2 cut(s) 99, 1413
BstSCI CCNGG 4 cut(s) 97, 1155, 1411, 1430
BstSLI GKGCMC 1 cut(s) 1411
BstUI CGCG 1 cut(s) 912
BstV1I GCAGC 7 cut(s) 122, 202, 514, 638, 793, 1259, 1298
BstX2I RGATCY 1 cut(s) 422
BstXI CCANNNNNNTGG 2 cut(s) 25, 451
BstYI RGATCY 1 cut(s) 422
BsuI GTATCC 1 cut(s) 763
BsuRI GGCC 3 cut(s) 29, 781, 1563
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 1098
BtsCI GGATG 4 cut(s) 14, 53, 271, 876
BtsI GCAGTG 3 cut(s) 18, 413, 610
BtsIMutI CAGTG 4 cut(s) 18, 324, 413, 610
BveI ACCTGC 1 cut(s) 1013
Cac8I GCNNGC 8 cut(s) 115, 159, 403, 445, 472, 496, 832, 1527
CaiI CAGNNNCTG 3 cut(s) 806, 1028, 1207
CfoI GCGC 5 cut(s) 271, 303, 476, 548, 912
Cfr10I RCCGGY 2 cut(s) 113, 401
Cfr13I GGNCC 2 cut(s) 235, 779
Csp6I GTAC 5 cut(s) 654, 874, 1225, 1391, 1435
CviAII CATG 2 cut(s) 25, 341
CviQI GTAC 5 cut(s) 654, 874, 1225, 1391, 1435
DdeI CTNAG 5 cut(s) 583, 697, 905, 920, 1533
DpnI GATC 4 cut(s) 424, 710, 1165, 1217
DpnII GATC 4 cut(s) 422, 708, 1163, 1215
DraI TTTAAA 1 cut(s) 1125
EaeI YGGCCR 1 cut(s) 27
Eco130I CCWWGG 4 cut(s) 24, 309, 378, 978
Eco31I GGTCTC 1 cut(s) 1444
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 97, 1411
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 4 cut(s) 24, 309, 378, 978
EcoT22I ATGCAT 2 cut(s) 1099, 1132
ErhI CCWWGG 4 cut(s) 24, 309, 378, 978
FaeI CATG 2 cut(s) 28, 344
FalI AAGNNNNNCTT 2 cut(s) 482, 514
FaqI GGGAC 1 cut(s) 734
FatI CATG 2 cut(s) 24, 340
FblI GTMKAC 1 cut(s) 1038
Fnu4HI GCNGC 8 cut(s) 111, 191, 528, 595, 627, 807, 1248, 1287
FokI GGATG 3 cut(s) 40, 278, 863
Fsp4HI GCNGC 8 cut(s) 111, 191, 528, 595, 627, 807, 1248, 1287
FspBI CTAG 4 cut(s) 251, 495, 732, 1352
GlaI GCGC 5 cut(s) 270, 302, 475, 547, 911
GluI GCNGC 8 cut(s) 111, 191, 528, 595, 627, 807, 1248, 1287
HaeII RGCGCY 2 cut(s) 477, 549
HaeIII GGCC 3 cut(s) 29, 781, 1563
HapII CCGG 7 cut(s) 114, 220, 233, 263, 402, 1157, 1431
HhaI GCGC 5 cut(s) 271, 303, 476, 548, 912
Hin1II CATG 2 cut(s) 28, 344
Hin6I GCGC 5 cut(s) 269, 301, 474, 546, 910
HinP1I GCGC 5 cut(s) 269, 301, 474, 546, 910
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 4 cut(s) 229, 246, 1297, 1366
HpaII CCGG 7 cut(s) 114, 220, 233, 263, 402, 1157, 1431
HphI GGTGA 6 cut(s) 81, 172, 266, 997, 1348, 1375
Hpy166II GTNNAC 3 cut(s) 1039, 1311, 1391
Hpy188I TCNGA 2 cut(s) 586, 1489
Hpy188III TCNNGA 4 cut(s) 220, 263, 437, 818
Hpy8I GTNNAC 3 cut(s) 1039, 1311, 1391
Hpy99I CGWCG 1 cut(s) 917
HpyAV CCTTC 6 cut(s) 961, 1132, 1195, 1276, 1560, 1564
HpyCH4III ACNGT 4 cut(s) 123, 775, 796, 900
HpyF10VI GCNNNNNNNGC 7 cut(s) 287, 552, 561, 600, 635, 806, 1253
HpyF3I CTNAG 5 cut(s) 583, 697, 905, 920, 1533
Hsp92II CATG 2 cut(s) 28, 344
HspAI GCGC 5 cut(s) 269, 301, 474, 546, 910
Kpn2I TCCGGA 2 cut(s) 219, 262
KpnI GGTACC 1 cut(s) 657
KroI GCCGGC 2 cut(s) 113, 401
KroNI GCCGGC 2 cut(s) 115, 403
Kzo9I GATC 4 cut(s) 422, 708, 1163, 1215
LmnI GCTCC 1 cut(s) 543
Lsp1109I GCAGC 7 cut(s) 122, 202, 514, 638, 793, 1259, 1298
LweI GCATC 5 cut(s) 256, 430, 793, 811, 1345
MaeI CTAG 4 cut(s) 251, 495, 732, 1352
MaeIII GTNAC 5 cut(s) 87, 321, 510, 1336, 1363
MalI GATC 4 cut(s) 424, 710, 1165, 1217
MboI GATC 4 cut(s) 422, 708, 1163, 1215
MboII GAAGA 3 cut(s) 65, 1068, 1357
MfeI CAATTG 3 cut(s) 720, 858, 1398
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 1411
MlsI TGGCCA 1 cut(s) 29
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 2 cut(s) 255, 1360
MmeI TCCRAC 1 cut(s) 521
Mox20I TGGCCA 1 cut(s) 29
Mph1103I ATGCAT 2 cut(s) 1099, 1132
MroI TCCGGA 2 cut(s) 219, 262
MroNI GCCGGC 2 cut(s) 113, 401
MscI TGGCCA 1 cut(s) 29
MseI TTAA 2 cut(s) 1092, 1124
MslI CAYNNNNRTG 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 1457
MspI CCGG 7 cut(s) 114, 220, 233, 263, 402, 1157, 1431
MspR9I CCNGG 4 cut(s) 99, 1157, 1413, 1432
MunI CAATTG 3 cut(s) 720, 858, 1398
Mva1269I GAATGC 1 cut(s) 345
MvaI CCWGG 2 cut(s) 99, 1413
MvnI CGCG 1 cut(s) 912
MwoI GCNNNNNNNGC 7 cut(s) 287, 552, 561, 600, 635, 806, 1253
NaeI GCCGGC 2 cut(s) 115, 403
NciI CCSGG 2 cut(s) 1157, 1432
NcoI CCATGG 1 cut(s) 24
NdeII GATC 4 cut(s) 422, 708, 1163, 1215
NgoMIV GCCGGC 2 cut(s) 113, 401
NheI GCTAGC 1 cut(s) 494
NlaIII CATG 2 cut(s) 28, 344
NlaIV GGNNCC 3 cut(s) 315, 655, 1408
NmuCI GTSAC 5 cut(s) 87, 321, 510, 1336, 1363
NsiI ATGCAT 2 cut(s) 1099, 1132
PaqCI CACCTGC 1 cut(s) 1013
PctI GAATGC 1 cut(s) 345
PdiI GCCGGC 2 cut(s) 115, 403
PfeI GAWTC 2 cut(s) 229, 1297
PflMI CCANNNNNTGG 2 cut(s) 722, 1207
PkrI GCNGC 8 cut(s) 112, 192, 529, 596, 628, 808, 1249, 1288
PleI GAGTC 2 cut(s) 254, 1360
PpsI GAGTC 2 cut(s) 254, 1360
PshBI ATTAAT 1 cut(s) 1092
PsiI TTATAA 1 cut(s) 693
Psp6I CCWGG 2 cut(s) 97, 1411
PspGI CCWGG 2 cut(s) 97, 1411
PspN4I GGNNCC 3 cut(s) 315, 655, 1408
PspPI GGNCC 2 cut(s) 235, 779
PstNI CAGNNNCTG 3 cut(s) 806, 1028, 1207
PsuI RGATCY 1 cut(s) 422
PvuII CAGCTG 1 cut(s) 1457
RsaI GTAC 5 cut(s) 655, 875, 1226, 1392, 1436
RsaNI GTAC 5 cut(s) 654, 874, 1225, 1391, 1435
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 2 cut(s) 1092, 1124
SatI GCNGC 8 cut(s) 111, 191, 528, 595, 627, 807, 1248, 1287
Sau3AI GATC 4 cut(s) 422, 708, 1163, 1215
Sau96I GGNCC 2 cut(s) 235, 779
SchI GAGTC 2 cut(s) 255, 1360
ScrFI CCNGG 4 cut(s) 99, 1157, 1413, 1432
SduI GDGCHC 1 cut(s) 1411
SfaNI GCATC 5 cut(s) 256, 430, 793, 811, 1345
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 5 cut(s) 184, 499, 598, 1384, 1565
SmoI CTYRAG 5 cut(s) 184, 499, 598, 1384, 1565
SpeI ACTAGT 1 cut(s) 731
SsiI CCGC 3 cut(s) 399, 555, 594
SspI AATATT 1 cut(s) 203
SspMI CTAG 4 cut(s) 251, 495, 732, 1352
StyD4I CCNGG 4 cut(s) 97, 1155, 1411, 1430
StyI CCWWGG 4 cut(s) 24, 309, 378, 978
TaaI ACNGT 4 cut(s) 123, 775, 796, 900
TaqI TCGA 1 cut(s) 736
TatI WGTACW 2 cut(s) 873, 1390
TauI GCSGC 1 cut(s) 597
TfiI GAWTC 2 cut(s) 229, 1297
Tru1I TTAA 2 cut(s) 1092, 1124
Tru9I TTAA 2 cut(s) 1092, 1124
TscAI CASTG 4 cut(s) 25, 324, 420, 610
TseFI GTSAC 5 cut(s) 87, 321, 510, 1336, 1363
TseI GCWGC 7 cut(s) 110, 190, 527, 626, 806, 1247, 1286
Tsp45I GTSAC 5 cut(s) 87, 321, 510, 1336, 1363
TspDTI ATGAA 2 cut(s) 404, 1101
TspGWI ACGGA 1 cut(s) 930
TspRI CASTG 4 cut(s) 25, 324, 420, 610
Van91I CCANNNNNTGG 2 cut(s) 722, 1207
VpaK11BI GGWCC 1 cut(s) 235
VspI ATTAAT 1 cut(s) 1092
XagI CCTNNNNNAGG 1 cut(s) 96
XapI RAATTY 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 1038
XspI CTAG 4 cut(s) 251, 495, 732, 1352
Zsp2I ATGCAT 2 cut(s) 1099, 1132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.