Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000427

Physical Location & Seq

Genomic Coordinates Forward (+)

19699381 .. 19702401

Gene Structure 3021 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1120400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGGCTACCACCAATTCAAGGCAGAGACTTTTCTCTCTCCTCTCGTTTCTTATTCTAATTCTTCAATCCAAAATCTCATCTGGGTCCGAAACCCCAATTGAAGAATCCACCATTGACGACCTCCAGATCGCTTTCAAGCTCAAGACTCTGACTTCACGGCAACTGGTTCAGTTCTACCTGGACAGAATCCAAACCCTCAACAGCCACCTTAGAGGGGTCCTGGAGGTGAACCCAGATGCGCTTTCCCAGGCCGATTCCGCCGACGAAAAACGCCGGAAATGGGAATCGAACCCATCAGCCAGAACCAGAGCACTGCCGAAGCTGCACGGGATTCCCATTTTGCTCAAGGACAACATTGGCACCAAGGACAAACTTAACACCACCGTCGGCTCCTTTGCGCTGTTGGGTTCCAAAGTTCCGAGGGATGCCGGGGTTGTGGCGAAGCTGAGGAGCTCTGGGGCTGTGATTCTGGGGAAGGGTAGCTTGAGCGAGTGGTCGTATATCCGGTCGTTTTCCGCGCCGTACGGATGGAGTGCCAGAGGCGGCCAGGGTGTGAATCCTTATAATTCATCGCTGGAAGTTTGTGGATCAAGTAGTGGACCGGCAATATCTGTATCTGCAAATATGGTAGCTGTGGCTTTAGGGACAGAGACTGACAGCTCCATCTTATGTCCGTCCAGTTATAACTCGGTAGTAGGCTTCAAGCCAACCGTTGGTCTCACTAGTCGAGCTGGAGTCATCCCAGTGAGTCCGAGACAGGACACAGTTGGGCCAATTTGTAGAACAGTGGCTGATGCTGTTCATGTCCTGGATGCCATTGTCGGTATTGATAGCAACGATATCGCAACGCATGAAGCATCGCAGTACATCCCGGTGGGTGGCTATGGACAGTTTCTCAAGGCTAATGGTCTCCAAGGAAAGAGATTGGGGATAGTAAGTCGCTTGTTTCATGAAGTCAAGGATGATTGTTTCTTGAATCAAACTTTTGAGCAGCACTTTACCACACTGAGGAAACAAGGTGCAGTTTTGGTTGGAAATCTTGAGATAGCCAACATTGAAGATATTCTCGATTTATCTTTGAGTGGTGAATTTGTAGCAATGTTAGCGGAATTCAAAATGGCCTTGAACCCATACCTAAGTGAACTAGTTGAATCTCCGGTGAGAACCTTGAGAGATGTGATAGCATTCAACAAGAACAACTCAGTTTTGGAAAAGATGAATGAATATGGACAAGATCTCTTAGAAGCTGCTGAGAAGACTAACGGTATAGGAGCACAAGAGCAAGAAATATTGTCAAATTTAGCAAGACTGTCCAGAGATGGATTTGAGAAGTTGATGAAAGATGAGAACCTAGATGCGCTGGTGACTTATTCCAACTCTGCTCATTCAATCCTTGGAATTGGTGGTTTTCCAGGCATTGTTGTGCCGGCAGGATATCATAATGTTGACAAGTACCCTTTCGGCATTTGCTTTGGGGGACTAAAGGGTTCGGAGCCAAAGCTGATTGAGGTTGCGTATGCATTTGAGCAAGCAACTAAGATCAGAAAGCCTCCGCCTGTTGGTTCTCGAGGCCAACTCCTCTCTACCACCGCTACTCCCACTCCTGAAGTTGAGTCATCAATACCCACCATTGCTGCAGCCACAAAAAGACCCGTCCTAGTCACTCAAGAGAACCTCAAGGAAAGCGCTTGCATGACAGCAGTCGACATGAATTTTTCTAGAAGGATACTTGGGGCAGTTTTAATAAGAATCAGTTTAATTATGCCGCTAATTGTCCATGAGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811

KEGG Pathways

Metabolic & Signaling

ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120

Pfam Domains

Protein Families

Amidase

Protein Analysis

596

Amino Acids

64.23

Weight (kDa)

6.78

Isoelectric Point (pI)

41.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Amidase	PF01425	55 - 456	1.1e-65	Amidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34880 AT4G34880
fragaria_vesca	FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica	MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica	Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis	pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis	RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata	RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora	Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii	Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa	Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha	Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana	Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	564, 684
AccB1I	GGYRCC	1 cut(s)	359
AccB7I	CCANNNNNTGG	1 cut(s)	713
AccI	GTMKAC	1 cut(s)	1704
AccII	CGCG	1 cut(s)	518
AciI	CCGC	7 cut(s)	258, 516, 543, 1106, 1553, 1590, 1766
AclWI	GGATC	1 cut(s)	595
AcoI	YGGCCR	1 cut(s)	544
AcsI	RAATTY	4 cut(s)	1088, 1109, 1297, 1711
AcuI	CTGAAG	1 cut(s)	1626
AfaI	GTAC	3 cut(s)	524, 866, 1454
AfeI	AGCGCT	1 cut(s)	1687
AfiI	CCNNNNNNNGG	7 cut(s)	18, 214, 280, 713, 878, 1008, 1559
AhlI	ACTAGT	2 cut(s)	722, 1144
AjnI	CCWGG	6 cut(s)	177, 219, 246, 546, 807, 1411
Alw21I	GWGCWC	4 cut(s)	313, 455, 1276, 1785
Alw26I	GTCTC	5 cut(s)	19, 644, 722, 748, 914
AlwI	GGATC	1 cut(s)	595
AlwNI	CAGNNNCTG	2 cut(s)	653, 791
Ama87I	CYCGRG	1 cut(s)	1566
Aor51HI	AGCGCT	1 cut(s)	1687
AoxI	GGCC	5 cut(s)	249, 544, 770, 1119, 1570
ApeKI	GCWGC	5 cut(s)	322, 991, 1247, 1634, 1637
ApoI	RAATTY	4 cut(s)	1088, 1109, 1297, 1711
Asp700I	GAANNNNTTC	1 cut(s)	1062
AspLEI	GCGC	5 cut(s)	241, 400, 520, 1360, 1688
AspS9I	GGNCC	4 cut(s)	84, 217, 599, 770
AsuC2I	CCSGG	2 cut(s)	430, 872
AsuHPI	GGTGA	4 cut(s)	238, 1097, 1171, 1375
AvaI	CYCGRG	1 cut(s)	1566
AvaII	GGWCC	3 cut(s)	84, 217, 599
BanI	GGYRCC	1 cut(s)	359
BanII	GRGCYC	2 cut(s)	455, 1785
BbsI	GAAGAC	1 cut(s)	1262
Bbv12I	GWGCWC	4 cut(s)	313, 455, 1276, 1785
BbvCI	CCTCAGC	1 cut(s)	446
BbvI	GCAGC	5 cut(s)	309, 1003, 1234, 1621, 1649
BccI	CCATC	4 cut(s)	301, 522, 671, 1313
BceAI	ACGGC	2 cut(s)	173, 505
BcgI	CGANNNNNNTGC	2 cut(s)	823, 857
BciT130I	CCWGG	6 cut(s)	179, 221, 248, 548, 809, 1413
BciVI	GTATCC	1 cut(s)	1719
BcnI	CCSGG	2 cut(s)	430, 872
BcoDI	GTCTC	5 cut(s)	19, 644, 722, 748, 914
BcuI	ACTAGT	2 cut(s)	722, 1144
BfaI	CTAG	5 cut(s)	723, 1145, 1352, 1658, 1719
BfmI	CTRYAG	1 cut(s)	1635
BfoI	RGCGCY	1 cut(s)	1689
BfuI	GTATCC	1 cut(s)	1719
BglII	AGATCT	1 cut(s)	1234
BisI	GCNGC	7 cut(s)	323, 544, 992, 1248, 1635, 1638, 1766
BlsI	GCNGC	7 cut(s)	324, 545, 993, 1249, 1636, 1639, 1767
Bme1390I	CCNGG	8 cut(s)	179, 221, 248, 430, 548, 809, 872, 1413
Bme18I	GGWCC	3 cut(s)	84, 217, 599
BmeT110I	CYCGRG	1 cut(s)	1566
BmgT120I	GGNCC	4 cut(s)	84, 217, 599, 770
BmiI	GGNNCC	6 cut(s)	85, 218, 361, 391, 409, 1494
BmrFI	CCNGG	8 cut(s)	179, 221, 248, 430, 548, 809, 872, 1413
BmrI	ACTGGG	1 cut(s)	737
BmsI	GCATC	6 cut(s)	226, 415, 784, 802, 866, 1345
BmuI	ACTGGG	1 cut(s)	737
BoxI	GACNNNNGTC	1 cut(s)	1700
BpiI	GAAGAC	1 cut(s)	1262
BplI	GAGNNNNNCTC	2 cut(s)	1560, 1592
BpmI	CTGGAG	3 cut(s)	107, 242, 753
Bpu10I	CCTNAGC	1 cut(s)	446
BpuEI	CTTGAG	8 cut(s)	125, 329, 505, 881, 1061, 1189, 1650, 1661
BpuMI	CCSGG	2 cut(s)	430, 872
BsaI	GGTCTC	2 cut(s)	722, 914
BsaJI	CCNNGG	7 cut(s)	246, 363, 419, 429, 547, 913, 1393
BsaWI	WCCGGW	2 cut(s)	504, 1156
BsaXI	ACNNNNNCTCC	4 cut(s)	1579, 1585, 1609, 1615
Bsc4I	CCNNNNNNNGG	7 cut(s)	18, 214, 280, 713, 878, 1008, 1559
Bse118I	RCCGGY	2 cut(s)	601, 1426
Bse1I	ACTGG	3 cut(s)	168, 678, 743
Bse3DI	GCAATG	2 cut(s)	1104, 1629
BseBI	CCWGG	6 cut(s)	179, 221, 248, 548, 809, 1413
BseDI	CCNNGG	7 cut(s)	246, 363, 419, 429, 547, 913, 1393
BseGI	GGATG	6 cut(s)	430, 533, 738, 817, 867, 967
BseLI	CCNNNNNNNGG	7 cut(s)	18, 214, 280, 713, 878, 1008, 1559
BseMI	GCAATG	2 cut(s)	1104, 1629
BseMII	CTCAG	4 cut(s)	437, 998, 1215, 1242
BseNI	ACTGG	3 cut(s)	168, 678, 743
BseRI	GAGGAG	3 cut(s)	29, 463, 1568
BseXI	GCAGC	5 cut(s)	309, 1003, 1234, 1621, 1649
BsgI	GTGCAG	2 cut(s)	308, 1041
Bsh1236I	CGCG	1 cut(s)	518
Bsh1285I	CGRYCG	1 cut(s)	509
BshFI	GGCC	5 cut(s)	251, 546, 772, 1121, 1572
BshNI	GGYRCC	1 cut(s)	359
BsiEI	CGRYCG	1 cut(s)	509
BsiHKAI	GWGCWC	4 cut(s)	313, 455, 1276, 1785
BsiHKCI	CYCGRG	1 cut(s)	1566
BsiSI	CCGG	7 cut(s)	274, 429, 505, 602, 872, 1157, 1427
BsiWI	CGTACG	1 cut(s)	522
BslFI	GGGAC	2 cut(s)	658, 1491
BslI	CCNNNNNNNGG	7 cut(s)	18, 214, 280, 713, 878, 1008, 1559
BsmAI	GTCTC	5 cut(s)	19, 644, 722, 748, 914
BsmFI	GGGAC	2 cut(s)	658, 1491
BsmI	GAATGC	1 cut(s)	1184
BsnI	GGCC	5 cut(s)	251, 546, 772, 1121, 1572
Bso31I	GGTCTC	2 cut(s)	722, 914
BsoBI	CYCGRG	1 cut(s)	1566
Bsp1286I	GDGCHC	4 cut(s)	313, 455, 1276, 1785
Bsp143I	GATC	4 cut(s)	126, 587, 1234, 1539
BspACI	CCGC	7 cut(s)	258, 516, 543, 1106, 1553, 1590, 1766
BspANI	GGCC	5 cut(s)	251, 546, 772, 1121, 1572
BspCNI	CTCAG	4 cut(s)	438, 999, 1214, 1243
BspFNI	CGCG	1 cut(s)	518
BspHI	TCATGA	1 cut(s)	949
BspLI	GGNNCC	6 cut(s)	85, 218, 361, 391, 409, 1494
BspMAI	CTGCAG	1 cut(s)	1639
BspPI	GGATC	1 cut(s)	595
BspT107I	GGYRCC	1 cut(s)	359
BspTNI	GGTCTC	2 cut(s)	722, 914
BsrDI	GCAATG	2 cut(s)	1104, 1629
BsrFI	RCCGGY	2 cut(s)	601, 1426
BsrI	ACTGG	3 cut(s)	168, 678, 743
BssAI	RCCGGY	2 cut(s)	601, 1426
BssECI	CCNNGG	7 cut(s)	246, 363, 419, 429, 547, 913, 1393
BssMI	GATC	4 cut(s)	126, 587, 1234, 1539
BssT1I	CCWWGG	3 cut(s)	363, 913, 1393
Bst2UI	CCWGG	6 cut(s)	179, 221, 248, 548, 809, 1413
Bst4CI	ACNGT	7 cut(s)	385, 712, 766, 787, 891, 1265, 1311
BstC8I	GCNNGC	3 cut(s)	1428, 1530, 1690
BstDEI	CTNAG	8 cut(s)	209, 446, 1007, 1136, 1201, 1240, 1251, 1536
BstF5I	GGATG	6 cut(s)	430, 533, 738, 817, 867, 967
BstFNI	CGCG	1 cut(s)	518
BstH2I	RGCGCY	1 cut(s)	1689
BstHHI	GCGC	5 cut(s)	241, 400, 520, 1360, 1688
BstKTI	GATC	4 cut(s)	129, 590, 1237, 1542
BstMAI	GTCTC	5 cut(s)	19, 644, 722, 748, 914
BstMBI	GATC	4 cut(s)	126, 587, 1234, 1539
BstMCI	CGRYCG	1 cut(s)	509
BstMWI	GCNNNNNNNGC	3 cut(s)	257, 322, 1103
BstNI	CCWGG	6 cut(s)	179, 221, 248, 548, 809, 1413
BstPAI	GACNNNNGTC	1 cut(s)	1700
BstSCI	CCNGG	8 cut(s)	177, 219, 246, 428, 546, 807, 870, 1411
BstSFI	CTRYAG	1 cut(s)	1635
BstUI	CGCG	1 cut(s)	518
BstV1I	GCAGC	5 cut(s)	309, 1003, 1234, 1621, 1649
BstV2I	GAAGAC	1 cut(s)	1262
BstX2I	RGATCY	1 cut(s)	1234
BstYI	RGATCY	1 cut(s)	1234
BsuI	GTATCC	1 cut(s)	1719
BsuRI	GGCC	5 cut(s)	251, 546, 772, 1121, 1572
BtgZI	GCGATG	2 cut(s)	555, 843
BtsCI	GGATG	6 cut(s)	430, 533, 738, 817, 867, 967
BtsI	GCAGTG	1 cut(s)	311
BtsIMutI	CAGTG	4 cut(s)	311, 750, 792, 1004
Cac8I	GCNNGC	3 cut(s)	1428, 1530, 1690
CaiI	CAGNNNCTG	2 cut(s)	653, 791
CciI	TCATGA	1 cut(s)	949
CfoI	GCGC	5 cut(s)	241, 400, 520, 1360, 1688
Cfr10I	RCCGGY	2 cut(s)	601, 1426
Cfr13I	GGNCC	4 cut(s)	84, 217, 599, 770
Csp6I	GTAC	3 cut(s)	523, 865, 1453
CviAII	CATG	6 cut(s)	803, 851, 950, 1693, 1708, 1778
CviQI	GTAC	3 cut(s)	523, 865, 1453
DdeI	CTNAG	8 cut(s)	209, 446, 1007, 1136, 1201, 1240, 1251, 1536
DpnI	GATC	4 cut(s)	128, 589, 1236, 1541
DpnII	GATC	4 cut(s)	126, 587, 1234, 1539
EaeI	YGGCCR	1 cut(s)	544
EciI	GGCGGA	2 cut(s)	247, 1542
Ecl136II	GAGCTC	2 cut(s)	453, 1783
Eco130I	CCWWGG	3 cut(s)	363, 913, 1393
Eco24I	GRGCYC	2 cut(s)	455, 1785
Eco31I	GGTCTC	2 cut(s)	722, 914
Eco32I	GATATC	2 cut(s)	841, 1436
Eco47I	GGWCC	3 cut(s)	84, 217, 599
Eco47III	AGCGCT	1 cut(s)	1687
Eco53kI	GAGCTC	2 cut(s)	453, 1783
Eco57I	CTGAAG	1 cut(s)	1626
Eco88I	CYCGRG	1 cut(s)	1566
EcoICRI	GAGCTC	2 cut(s)	453, 1783
EcoO109I	RGGNCCY	1 cut(s)	217
EcoRI	GAATTC	1 cut(s)	1109
EcoRII	CCWGG	6 cut(s)	177, 219, 246, 546, 807, 1411
EcoRV	GATATC	2 cut(s)	841, 1436
EcoT14I	CCWWGG	3 cut(s)	363, 913, 1393
EcoT22I	ATGCAT	1 cut(s)	1522
EcoT38I	GRGCYC	2 cut(s)	455, 1785
ErhI	CCWWGG	3 cut(s)	363, 913, 1393
FaeI	CATG	6 cut(s)	806, 854, 953, 1696, 1711, 1781
FalI	AAGNNNNNCTT	2 cut(s)	467, 499
FaqI	GGGAC	2 cut(s)	658, 1491
FatI	CATG	6 cut(s)	802, 850, 949, 1692, 1707, 1777
FblI	GTMKAC	1 cut(s)	1704
Fnu4HI	GCNGC	7 cut(s)	323, 544, 992, 1248, 1635, 1638, 1766
FokI	GGATG	6 cut(s)	437, 540, 725, 824, 854, 974
FriOI	GRGCYC	2 cut(s)	455, 1785
Fsp4HI	GCNGC	7 cut(s)	323, 544, 992, 1248, 1635, 1638, 1766
FspBI	CTAG	5 cut(s)	723, 1145, 1352, 1658, 1719
GlaI	GCGC	5 cut(s)	240, 399, 519, 1359, 1687
GluI	GCNGC	7 cut(s)	323, 544, 992, 1248, 1635, 1638, 1766
GsuI	CTGGAG	3 cut(s)	107, 242, 753
HaeII	RGCGCY	1 cut(s)	1689
HaeIII	GGCC	5 cut(s)	251, 546, 772, 1121, 1572
HapII	CCGG	7 cut(s)	274, 429, 505, 602, 872, 1157, 1427
HhaI	GCGC	5 cut(s)	241, 400, 520, 1360, 1688
Hin1II	CATG	6 cut(s)	806, 854, 953, 1696, 1711, 1781
Hin6I	GCGC	5 cut(s)	239, 398, 518, 1358, 1686
HinP1I	GCGC	5 cut(s)	239, 398, 518, 1358, 1686
HincII	GTYRAC	2 cut(s)	1447, 1705
HindII	GTYRAC	2 cut(s)	1447, 1705
HpaII	CCGG	7 cut(s)	274, 429, 505, 602, 872, 1157, 1427
HphI	GGTGA	4 cut(s)	238, 1097, 1171, 1375
Hpy166II	GTNNAC	5 cut(s)	229, 599, 1142, 1447, 1705
Hpy188I	TCNGA	6 cut(s)	88, 150, 420, 753, 1492, 1544
Hpy8I	GTNNAC	5 cut(s)	229, 599, 1142, 1447, 1705
Hpy99I	CGWCG	2 cut(s)	266, 389
HpyAV	CCTTC	2 cut(s)	469, 1716
HpyCH4III	ACNGT	7 cut(s)	385, 712, 766, 787, 891, 1265, 1311
HpyCH4V	TGCA	6 cut(s)	325, 620, 1022, 1520, 1637, 1692
HpyF10VI	GCNNNNNNNGC	3 cut(s)	257, 322, 1103
HpyF3I	CTNAG	8 cut(s)	209, 446, 1007, 1136, 1201, 1240, 1251, 1536
Hsp92II	CATG	6 cut(s)	806, 854, 953, 1696, 1711, 1781
HspAI	GCGC	5 cut(s)	239, 398, 518, 1358, 1686
KroI	GCCGGC	1 cut(s)	1426
KroNI	GCCGGC	1 cut(s)	1428
Kzo9I	GATC	4 cut(s)	126, 587, 1234, 1539
LmnI	GCTCC	5 cut(s)	395, 450, 665, 1271, 1492
Lsp1109I	GCAGC	5 cut(s)	309, 1003, 1234, 1621, 1649
LweI	GCATC	6 cut(s)	226, 415, 784, 802, 866, 1345
MaeI	CTAG	5 cut(s)	723, 1145, 1352, 1658, 1719
MaeIII	GTNAC	2 cut(s)	1363, 1660
MalI	GATC	4 cut(s)	128, 589, 1236, 1541
MboI	GATC	4 cut(s)	126, 587, 1234, 1539
MboII	GAAGA	4 cut(s)	53, 113, 1070, 1267
MfeI	CAATTG	1 cut(s)	96
MflI	RGATCY	1 cut(s)	1234
MhlI	GDGCHC	4 cut(s)	313, 455, 1276, 1785
MlyI	GAGTC	4 cut(s)	139, 744, 757, 1622
MmeI	TCCRAC	2 cut(s)	1012, 1398
Mph1103I	ATGCAT	1 cut(s)	1522
MroNI	GCCGGC	1 cut(s)	1426
MroXI	GAANNNNTTC	1 cut(s)	1062
MseI	TTAA	3 cut(s)	375, 1742, 1757
MslI	CAYNNNNRTG	3 cut(s)	743, 872, 1421
MspI	CCGG	7 cut(s)	274, 429, 505, 602, 872, 1157, 1427
MspR9I	CCNGG	8 cut(s)	179, 221, 248, 430, 548, 809, 872, 1413
MunI	CAATTG	1 cut(s)	96
Mva1269I	GAATGC	1 cut(s)	1184
MvaI	CCWGG	6 cut(s)	179, 221, 248, 548, 809, 1413
MvnI	CGCG	1 cut(s)	518
MwoI	GCNNNNNNNGC	3 cut(s)	257, 322, 1103
NaeI	GCCGGC	1 cut(s)	1428
NciI	CCSGG	2 cut(s)	430, 872
NdeII	GATC	4 cut(s)	126, 587, 1234, 1539
NgoMIV	GCCGGC	1 cut(s)	1426
NlaIII	CATG	6 cut(s)	806, 854, 953, 1696, 1711, 1781
NlaIV	GGNNCC	6 cut(s)	85, 218, 361, 391, 409, 1494
NmuCI	GTSAC	2 cut(s)	1363, 1660
NsiI	ATGCAT	1 cut(s)	1522
PaeR7I	CTCGAG	1 cut(s)	1566
PagI	TCATGA	1 cut(s)	949
PctI	GAATGC	1 cut(s)	1184
PdiI	GCCGGC	1 cut(s)	1428
PdmI	GAANNNNTTC	1 cut(s)	1062
Pfl23II	CGTACG	1 cut(s)	522
PflMI	CCANNNNNTGG	1 cut(s)	713
PfoI	TCCNGGA	2 cut(s)	219, 807
PkrI	GCNGC	7 cut(s)	324, 545, 993, 1249, 1636, 1639, 1767
PleI	GAGTC	4 cut(s)	139, 743, 756, 1621
PpsI	GAGTC	4 cut(s)	139, 743, 756, 1621
PpuMI	RGGWCCY	1 cut(s)	217
PshAI	GACNNNNGTC	1 cut(s)	1700
PsiI	TTATAA	2 cut(s)	564, 684
Psp124BI	GAGCTC	2 cut(s)	455, 1785
Psp5II	RGGWCCY	1 cut(s)	217
Psp6I	CCWGG	6 cut(s)	177, 219, 246, 546, 807, 1411
PspGI	CCWGG	6 cut(s)	177, 219, 246, 546, 807, 1411
PspLI	CGTACG	1 cut(s)	522
PspN4I	GGNNCC	6 cut(s)	85, 218, 361, 391, 409, 1494
PspPI	GGNCC	4 cut(s)	84, 217, 599, 770
PspPPI	RGGWCCY	1 cut(s)	217
PstI	CTGCAG	1 cut(s)	1639
PstNI	CAGNNNCTG	2 cut(s)	653, 791
PsuI	RGATCY	1 cut(s)	1234
RsaI	GTAC	3 cut(s)	524, 866, 1454
RsaNI	GTAC	3 cut(s)	523, 865, 1453
RseI	CAYNNNNRTG	3 cut(s)	743, 872, 1421
SacI	GAGCTC	2 cut(s)	455, 1785
SalI	GTCGAC	1 cut(s)	1703
SaqAI	TTAA	3 cut(s)	375, 1742, 1757
SatI	GCNGC	7 cut(s)	323, 544, 992, 1248, 1635, 1638, 1766
Sau3AI	GATC	4 cut(s)	126, 587, 1234, 1539
Sau96I	GGNCC	4 cut(s)	84, 217, 599, 770
SchI	GAGTC	4 cut(s)	139, 744, 757, 1622
ScrFI	CCNGG	8 cut(s)	179, 221, 248, 430, 548, 809, 872, 1413
SduI	GDGCHC	4 cut(s)	313, 455, 1276, 1785
SfaNI	GCATC	6 cut(s)	226, 415, 784, 802, 866, 1345
SfcI	CTRYAG	1 cut(s)	1635
Sfr274I	CTCGAG	1 cut(s)	1566
SinI	GGWCC	3 cut(s)	84, 217, 599
SlaI	CTCGAG	1 cut(s)	1566
SmiMI	CAYNNNNRTG	3 cut(s)	743, 872, 1421
SmlI	CTYRAG	9 cut(s)	140, 344, 484, 896, 1040, 1168, 1566, 1665, 1676
SmoI	CTYRAG	9 cut(s)	140, 344, 484, 896, 1040, 1168, 1566, 1665, 1676
SpeI	ACTAGT	2 cut(s)	722, 1144
SsiI	CCGC	7 cut(s)	258, 516, 543, 1106, 1553, 1590, 1766
SspI	AATATT	1 cut(s)	1290
SspMI	CTAG	5 cut(s)	723, 1145, 1352, 1658, 1719
SstI	GAGCTC	2 cut(s)	455, 1785
StyD4I	CCNGG	8 cut(s)	177, 219, 246, 428, 546, 807, 870, 1411
StyI	CCWWGG	3 cut(s)	363, 913, 1393
TaaI	ACNGT	7 cut(s)	385, 712, 766, 787, 891, 1265, 1311
TaqI	TCGA	5 cut(s)	287, 727, 1068, 1567, 1704
TatI	WGTACW	1 cut(s)	864
TauI	GCSGC	2 cut(s)	546, 1768
Tru1I	TTAA	3 cut(s)	375, 1742, 1757
Tru9I	TTAA	3 cut(s)	375, 1742, 1757
TscAI	CASTG	4 cut(s)	318, 750, 792, 1011
TseFI	GTSAC	2 cut(s)	1363, 1660
TseI	GCWGC	5 cut(s)	322, 991, 1247, 1634, 1637
Tsp45I	GTSAC	2 cut(s)	1363, 1660
TspDTI	ATGAA	9 cut(s)	558, 791, 867, 938, 966, 1232, 1236, 1352, 1724
TspGWI	ACGGA	2 cut(s)	540, 663
TspRI	CASTG	4 cut(s)	318, 750, 792, 1011
Van91I	CCANNNNNTGG	1 cut(s)	713
VpaK11BI	GGWCC	3 cut(s)	84, 217, 599
XapI	RAATTY	4 cut(s)	1088, 1109, 1297, 1711
XbaI	TCTAGA	1 cut(s)	1718
XhoI	CTCGAG	1 cut(s)	1566
XmiI	GTMKAC	1 cut(s)	1704
XmnI	GAANNNNTTC	1 cut(s)	1062
XspI	CTAG	5 cut(s)	723, 1145, 1352, 1658, 1719
Zsp2I	ATGCAT	1 cut(s)	1522

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.