Rmu_sc0006635.1_g000002

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006635.1
OG0000427
Physical Location & Seq
Forward (+)
9022 .. 11164
2143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006635.1_g000002.1.cds

Sequence Viewer

Length: 957 bp
ctggaaatagcaaacattgatactatcttgaattttaatttgagtggagaagcaatagcaacagtggctgagttcaaactagccataaatgcatatctagaagaccttgtggtgtctccagttagatctttggcagatatcatagccttcaacttgaagttttctggtgtcgaaatgatcaaggaatttggccaagacatttttcttgcagcacaagccacaaatgggataggcaacaatgagagggcagcattgttaaatttagcaaagctgacaaaagatggatttgagaaactaatgacatacaacaggctagatgcattggtgactcccggtgcagatattgctcctgtacttgcaattgggggattcccaggcattagtgtccctgctggatatgacaagaagggtgtgcctttcggcataacttttgggggtctaaagggatctgaaccaaagcttatacagattgcctacggttctttctctctcagactcccaagccttccttcccctttcttcgatctcacaccctccgataagctcatatcgcacgccggcggtctcttccgtacttcgccgttccgcctcctctttcagattgagatcgacgagttagagcggcgtcgccaccttcctagctttgcctccgtagttgtcgttctcccttccctcctccgattctccaccaccttcaccgtcaccgacacttttgggagtccgaaacgatctctcggaattaggcaggagcttccataccggcctctcatttctctctgtcgccggaatctccaattggcttcgaacttgatgccggttcggaatggagcttcggaagacaacggcggcggttccgtgccaagaattaccgatgagattctcggcttcgtcgctccgatttcaaagcttccagactgttggaccggctttcctatacccactcgactacgatggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

318

Amino Acids

34.61

Weight (kDa)

8.53

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 622
AciI CCGC 5 cut(s) 561, 586, 622, 846, 849
AclWI GGATC 1 cut(s) 454
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 3 cut(s) 31, 185, 259
AcyI GRCGYC 1 cut(s) 625
AfaI GTAC 2 cut(s) 354, 574
AfiI CCNNNNNNNGG 1 cut(s) 225
AgsI TTSAA 5 cut(s) 31, 76, 151, 157, 903
AjnI CCWGG 1 cut(s) 373
AjuI GAANNNNNNNTTGG 6 cut(s) 186, 218, 493, 525, 779, 811
AluBI AGCT 7 cut(s) 271, 460, 544, 642, 751, 830, 907
AluI AGCT 7 cut(s) 271, 460, 544, 642, 751, 830, 907
Alw26I GTCTC 2 cut(s) 120, 569
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 68
AoxI GGCC 2 cut(s) 190, 761
ApeKI GCWGC 2 cut(s) 209, 248
ApoI RAATTY 3 cut(s) 31, 185, 259
AspS9I GGNCC 1 cut(s) 921
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 3 cut(s) 337, 688, 694
AsuII TTCGAA 1 cut(s) 803
AvaII GGWCC 1 cut(s) 921
BalI TGGCCA 1 cut(s) 192
BbsI GAAGAC 2 cut(s) 108, 843
BbvI GCAGC 2 cut(s) 221, 260
BccI CCATC 2 cut(s) 275, 945
BceAI ACGGC 2 cut(s) 565, 859
BcgI CGANNNNNNTGC 2 cut(s) 793, 827
BciT130I CCWGG 1 cut(s) 375
BclI TGATCA 1 cut(s) 177
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 120, 569
BfaI CTAG 4 cut(s) 80, 98, 314, 639
BglII AGATCT 1 cut(s) 125
BisI GCNGC 4 cut(s) 210, 249, 623, 847
BlsI GCNGC 4 cut(s) 211, 250, 624, 848
Bme1390I CCNGG 2 cut(s) 333, 375
Bme18I GGWCC 1 cut(s) 921
BmgT120I GGNCC 1 cut(s) 921
BmiI GGNNCC 1 cut(s) 853
BmrFI CCNGG 2 cut(s) 333, 375
BmsI GCATC 2 cut(s) 307, 801
BpiI GAAGAC 2 cut(s) 108, 843
BpmI CTGGAG 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 803
BpuMI CCSGG 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 605
BsaHI GRCGYC 1 cut(s) 625
BsaI GGTCTC 1 cut(s) 569
BsaJI CCNNGG 1 cut(s) 373
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse118I RCCGGY 4 cut(s) 557, 759, 814, 923
Bse1I ACTGG 1 cut(s) 119
Bse8I GATNNNNATC 1 cut(s) 605
BseBI CCWGG 1 cut(s) 375
BseDI CCNNGG 1 cut(s) 373
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMII CTCAG 2 cut(s) 60, 505
BseNI ACTGG 1 cut(s) 119
BseRI GAGGAG 2 cut(s) 581, 665
BseXI GCAGC 2 cut(s) 221, 260
BsgI GTGCAG 1 cut(s) 357
BshFI GGCC 2 cut(s) 192, 763
BsiSI CCGG 6 cut(s) 333, 558, 760, 784, 815, 924
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 225
BsmAI GTCTC 2 cut(s) 120, 569
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 2 cut(s) 192, 763
Bso31I GGTCTC 1 cut(s) 569
Bsp119I TTCGAA 1 cut(s) 803
Bsp143I GATC 6 cut(s) 125, 177, 446, 523, 606, 728
BspACI CCGC 5 cut(s) 561, 586, 622, 846, 849
BspANI GGCC 2 cut(s) 192, 763
BspCNI CTCAG 2 cut(s) 61, 504
BspLI GGNNCC 1 cut(s) 853
BspPI GGATC 1 cut(s) 454
BspT104I TTCGAA 1 cut(s) 803
BspTNI GGTCTC 1 cut(s) 569
BsrBI CCGCTC 1 cut(s) 622
BsrFI RCCGGY 4 cut(s) 557, 759, 814, 923
BsrI ACTGG 1 cut(s) 119
BssAI RCCGGY 4 cut(s) 557, 759, 814, 923
BssECI CCNNGG 1 cut(s) 373
BssMI GATC 6 cut(s) 125, 177, 446, 523, 606, 728
BssNI GRCGYC 1 cut(s) 625
Bst2UI CCWGG 1 cut(s) 375
Bst4CI ACNGT 4 cut(s) 64, 479, 700, 917
Bst6I CTCTTC 1 cut(s) 572
BstACI GRCGYC 1 cut(s) 625
BstAPI GCANNNNNTGC 1 cut(s) 344
BstBI TTCGAA 1 cut(s) 803
BstC8I GCNNGC 2 cut(s) 555, 559
BstDEI CTNAG 2 cut(s) 69, 491
BstKTI GATC 6 cut(s) 128, 180, 449, 526, 609, 731
BstMAI GTCTC 2 cut(s) 120, 569
BstMBI GATC 6 cut(s) 125, 177, 446, 523, 606, 728
BstMWI GCNNNNNNNGC 5 cut(s) 65, 89, 215, 344, 550
BstNI CCWGG 1 cut(s) 375
BstSCI CCNGG 2 cut(s) 331, 373
BstV1I GCAGC 2 cut(s) 221, 260
BstV2I GAAGAC 2 cut(s) 108, 843
BstX2I RGATCY 2 cut(s) 125, 446
BstXI CCANNNNNNTGG 1 cut(s) 918
BstYI RGATCY 2 cut(s) 125, 446
BsuRI GGCC 2 cut(s) 192, 763
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 2 cut(s) 555, 559
CaiI CAGNNNCTG 1 cut(s) 68
Cfr10I RCCGGY 4 cut(s) 557, 759, 814, 923
Cfr13I GGNCC 1 cut(s) 921
CseI GACGC 1 cut(s) 614
Csp6I GTAC 2 cut(s) 353, 573
CviQI GTAC 2 cut(s) 353, 573
DdeI CTNAG 2 cut(s) 69, 491
DpnI GATC 6 cut(s) 127, 179, 448, 525, 608, 730
DpnII GATC 6 cut(s) 125, 177, 446, 523, 606, 728
EaeI YGGCCR 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
EciI GGCGGA 1 cut(s) 575
Eco31I GGTCTC 1 cut(s) 569
Eco32I GATATC 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 921
EcoRII CCWGG 1 cut(s) 373
EcoRV GATATC 1 cut(s) 139
EcoT22I ATGCAT 2 cut(s) 94, 322
FalI AAGNNNNNCTT 2 cut(s) 493, 525
FaqI GGGAC 1 cut(s) 371
FbaI TGATCA 1 cut(s) 177
Fnu4HI GCNGC 4 cut(s) 210, 249, 623, 847
Fsp4HI GCNGC 4 cut(s) 210, 249, 623, 847
FspBI CTAG 4 cut(s) 80, 98, 314, 639
GluI GCNGC 4 cut(s) 210, 249, 623, 847
GsuI CTGGAG 1 cut(s) 102
HaeIII GGCC 2 cut(s) 192, 763
HapII CCGG 6 cut(s) 333, 558, 760, 784, 815, 924
HgaI GACGC 1 cut(s) 614
Hin1I GRCGYC 1 cut(s) 625
HindIII AAGCTT 2 cut(s) 458, 905
HinfI GANTC 7 cut(s) 328, 369, 495, 681, 718, 787, 877
HpaII CCGG 6 cut(s) 333, 558, 760, 784, 815, 924
HphI GGTGA 3 cut(s) 337, 688, 694
Hpy188III TCNNGA 3 cut(s) 28, 98, 911
Hpy99I CGWCG 3 cut(s) 614, 630, 893
HpyAV CCTTC 7 cut(s) 157, 400, 515, 519, 644, 678, 703
HpyCH4III ACNGT 4 cut(s) 64, 479, 700, 917
HpyCH4V TGCA 5 cut(s) 92, 209, 320, 338, 359
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 89, 215, 344, 550
HpyF3I CTNAG 2 cut(s) 69, 491
Hsp92I GRCGYC 1 cut(s) 625
KroI GCCGGC 1 cut(s) 557
KroNI GCCGGC 1 cut(s) 559
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 6 cut(s) 125, 177, 446, 523, 606, 728
LmnI GCTCC 4 cut(s) 352, 748, 827, 898
Lsp1109I GCAGC 2 cut(s) 221, 260
LweI GCATC 2 cut(s) 307, 801
MaeI CTAG 4 cut(s) 80, 98, 314, 639
MaeIII GTNAC 2 cut(s) 325, 700
MalI GATC 6 cut(s) 127, 179, 448, 525, 608, 730
MbiI CCGCTC 1 cut(s) 622
MboI GATC 6 cut(s) 125, 177, 446, 523, 606, 728
MboII GAAGA 4 cut(s) 113, 511, 559, 848
MfeI CAATTG 2 cut(s) 360, 794
MflI RGATCY 2 cut(s) 125, 446
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 8 cut(s) 31, 37, 185, 259, 360, 738, 794, 864
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 3 cut(s) 322, 489, 727
MmeI TCCRAC 1 cut(s) 899
MnlI CCTC 8 cut(s) 237, 544, 599, 602, 658, 683, 686, 774
Mox20I TGGCCA 1 cut(s) 192
Mph1103I ATGCAT 2 cut(s) 94, 322
MreI CGCCGGCG 1 cut(s) 557
MroNI GCCGGC 1 cut(s) 557
MscI TGGCCA 1 cut(s) 192
MseI TTAA 2 cut(s) 36, 257
Msp20I TGGCCA 1 cut(s) 192
MspI CCGG 6 cut(s) 333, 558, 760, 784, 815, 924
MspR9I CCNGG 2 cut(s) 333, 375
MunI CAATTG 2 cut(s) 360, 794
MvaI CCWGG 1 cut(s) 375
MwoI GCNNNNNNNGC 5 cut(s) 65, 89, 215, 344, 550
NaeI GCCGGC 1 cut(s) 559
NciI CCSGG 1 cut(s) 333
NdeII GATC 6 cut(s) 125, 177, 446, 523, 606, 728
NgoMIV GCCGGC 1 cut(s) 557
NlaIV GGNNCC 1 cut(s) 853
NmeAIII GCCGAG 1 cut(s) 861
NmuCI GTSAC 2 cut(s) 325, 700
NsiI ATGCAT 2 cut(s) 94, 322
NspV TTCGAA 1 cut(s) 803
PdiI GCCGGC 1 cut(s) 559
PfeI GAWTC 4 cut(s) 369, 681, 787, 877
PkrI GCNGC 4 cut(s) 211, 250, 624, 848
PleI GAGTC 3 cut(s) 322, 489, 726
PpsI GAGTC 3 cut(s) 322, 489, 726
Psp6I CCWGG 1 cut(s) 373
PspGI CCWGG 1 cut(s) 373
PspN4I GGNNCC 1 cut(s) 853
PspPI GGNCC 1 cut(s) 921
PsrI GAACNNNNNNTAC 2 cut(s) 645, 677
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 2 cut(s) 125, 446
RsaI GTAC 2 cut(s) 354, 574
RsaNI GTAC 2 cut(s) 353, 573
SaqAI TTAA 2 cut(s) 36, 257
SatI GCNGC 4 cut(s) 210, 249, 623, 847
Sau3AI GATC 6 cut(s) 125, 177, 446, 523, 606, 728
Sau96I GGNCC 1 cut(s) 921
SchI GAGTC 3 cut(s) 322, 489, 727
ScrFI CCNGG 2 cut(s) 333, 375
SfaNI GCATC 2 cut(s) 307, 801
SfuI TTCGAA 1 cut(s) 803
SgrAI CRCCGGYG 1 cut(s) 557
SinI GGWCC 1 cut(s) 921
Sse9I AATT 8 cut(s) 31, 37, 185, 259, 360, 738, 794, 864
SsiI CCGC 5 cut(s) 561, 586, 622, 846, 849
SspMI CTAG 4 cut(s) 80, 98, 314, 639
StyD4I CCNGG 2 cut(s) 331, 373
TaaI ACNGT 4 cut(s) 64, 479, 700, 917
TaqI TCGA 5 cut(s) 171, 522, 609, 803, 943
TasI AATT 8 cut(s) 31, 37, 185, 259, 360, 738, 794, 864
TatI WGTACW 1 cut(s) 352
TauI GCSGC 2 cut(s) 625, 849
TfiI GAWTC 4 cut(s) 369, 681, 787, 877
Tru1I TTAA 2 cut(s) 36, 257
Tru9I TTAA 2 cut(s) 36, 257
TscAI CASTG 1 cut(s) 69
TseFI GTSAC 2 cut(s) 325, 700
TseI GCWGC 2 cut(s) 209, 248
Tsp45I GTSAC 2 cut(s) 325, 700
TspGWI ACGGA 3 cut(s) 560, 640, 844
TspRI CASTG 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 921
XapI RAATTY 3 cut(s) 31, 185, 259
XbaI TCTAGA 1 cut(s) 97
XspI CTAG 4 cut(s) 80, 98, 314, 639
Zsp2I ATGCAT 2 cut(s) 94, 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.