RchiOBHm_Chr6g0244751

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000427
Physical Location & Seq
Reverse (-)
753020 .. 755534
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21934

Sequence Viewer

Length: 1563 bp
ATGATAATTTCCTCATCCACTGCCATGGCGAAATTCCCTCACATCTCATGCTTATTGCTTCTCTTCACATTGTCATCTCTTCTAGGCACATTCGCAGCCCGCAGACTCTCACTAAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTCAAGCACAACCAACTCACCTCGAGGCAACTTGTTCAATATTACCTTGCCCAAATCCGAAAATTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAATCCAGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGATCAAGGACAACATTGCAACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCGCTGTTTGGATCTGTCGTGCCTCGTGATGCCGGCGTGGTGGAGAAGTTGCGGAAAGCAGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGTCTACTAAAACCCCCTCTGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTGTCATTAGGTACCGAGACAAGTGGTTCAATCTTATGTCCATCAAGTTTGAACTCAGTTGTGGGCCTCAAACCAACAATCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCTAAGACAGGATACTGTTGGGCCAATTTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGGATCGACCACAATGACATTGCAACAATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGTGGAAAAAGAATAGGGATATTCGGAGCCTTCCATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGAGCTGTTTTGGTAGACAAATTGGAAATTGCTAATTTGACTGAAATCTATTCTAGAAGCGATGAATATGCTGCATTGTATATTGAGTGCAAAATAACCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCGGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAAGAAACACCAGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACCTATTTTTAGCAGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCTGGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTGAAATTGGTGACAAAGAAGAAGCTAGATGTTGTGGTGTCTCCTTACGCAAGTTTGTCACGTGTACTTGCAATTGGGAGAGCCCCAGGTCTAATAGTTCCAGCTGGATACGAAAACAATGAGGAACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATCGAGATTGCATATGCTTTTGAGCAAGCCACTAAGATTAGGAAGCCTCCTTCATTGAAAGGCTTCAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

520

Amino Acids

56.08

Weight (kDa)

9.53

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 60 - 456 4e-71 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 169
Acc65I GGTACC 1 cut(s) 641
AccB1I GGYRCC 2 cut(s) 298, 641
AccB7I CCANNNNNTGG 1 cut(s) 1195
AccI GTMKAC 2 cut(s) 509, 1026
AccII CGCG 1 cut(s) 900
AciI CCGC 7 cut(s) 100, 384, 448, 543, 582, 594, 629
AclWI GGATC 3 cut(s) 415, 830, 1146
AcsI RAATTY 1 cut(s) 32
AcuI CTGAAG 1 cut(s) 1260
AcvI CACGTG 1 cut(s) 1376
AfaI GTAC 3 cut(s) 643, 863, 1380
AfiI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
AflIII ACRYGT 1 cut(s) 1375
AhlI ACTAGT 1 cut(s) 719
AjnI CCWGG 1 cut(s) 1399
AluBI AGCT 9 cut(s) 142, 483, 1016, 1238, 1273, 1339, 1418, 1445, 1486
AluI AGCT 9 cut(s) 142, 483, 1016, 1238, 1273, 1339, 1418, 1445, 1486
Alw26I GTCTC 3 cut(s) 641, 845, 1359
AlwI GGATC 3 cut(s) 415, 830, 1146
AlwNI CAGNNNCTG 2 cut(s) 794, 1195
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 2 cut(s) 694, 767
ApeKI GCWGC 7 cut(s) 95, 591, 614, 626, 794, 1082, 1235
ApoI RAATTY 1 cut(s) 32
Asp700I GAANNNNTTC 1 cut(s) 1550
Asp718I GGTACC 1 cut(s) 641
AspLEI GCGC 5 cut(s) 256, 288, 400, 536, 900
AspS9I GGNCC 3 cut(s) 291, 694, 767
AsuC2I CCSGG 2 cut(s) 218, 1145
AsuHPI GGTGA 3 cut(s) 157, 251, 1336
AsuNHI GCTAGC 1 cut(s) 479
AvaI CYCGRG 1 cut(s) 169
AvaII GGWCC 1 cut(s) 291
BanI GGYRCC 2 cut(s) 298, 641
BanII GRGCYC 1 cut(s) 1399
BauI CACGAG 1 cut(s) 420
BbrPI CACGTG 1 cut(s) 1376
BbvI GCAGC 7 cut(s) 107, 603, 626, 638, 781, 1069, 1247
BccI CCATC 1 cut(s) 679
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BciT130I CCWGG 1 cut(s) 1401
BciVI GTATCC 2 cut(s) 751, 1415
BclI TGATCA 1 cut(s) 342
BcnI CCSGG 2 cut(s) 218, 1145
BcoDI GTCTC 3 cut(s) 641, 845, 1359
BcuI ACTAGT 1 cut(s) 719
BfaI CTAG 6 cut(s) 83, 236, 480, 720, 1065, 1340
BfoI RGCGCY 1 cut(s) 537
BfuI GTATCC 2 cut(s) 751, 1415
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
BlsI GCNGC 8 cut(s) 97, 584, 593, 616, 628, 796, 1084, 1237
Bme1390I CCNGG 3 cut(s) 218, 1145, 1401
Bme18I GGWCC 1 cut(s) 291
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 3 cut(s) 291, 694, 767
BmiI GGNNCC 4 cut(s) 300, 643, 937, 1440
BmrFI CCNGG 3 cut(s) 218, 1145, 1401
BmsI GCATC 4 cut(s) 241, 415, 781, 799
BmtI GCTAGC 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 122
BpuEI CTTGAG 2 cut(s) 505, 571
BpuMI CCSGG 2 cut(s) 218, 1145
BsaAI YACGTR 1 cut(s) 1376
BsaJI CCNNGG 7 cut(s) 24, 294, 363, 966, 1143, 1155, 1399
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
Bse118I RCCGGY 2 cut(s) 386, 428
Bse1I ACTGG 2 cut(s) 302, 1200
Bse3DI GCAATG 3 cut(s) 354, 837, 961
BseBI CCWGG 1 cut(s) 1401
BseDI CCNNGG 7 cut(s) 24, 294, 363, 966, 1143, 1155, 1399
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
BseMI GCAATG 3 cut(s) 354, 837, 961
BseMII CTCAG 4 cut(s) 585, 699, 907, 992
BseNI ACTGG 2 cut(s) 302, 1200
BseRI GAGGAG 1 cut(s) 1026
BseXI GCAGC 7 cut(s) 107, 603, 626, 638, 781, 1069, 1247
Bsh1236I CGCG 1 cut(s) 900
BshFI GGCC 2 cut(s) 696, 769
BshNI GGYRCC 2 cut(s) 298, 641
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 4 cut(s) 218, 387, 429, 1145
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 5 cut(s) 528, 710, 819, 875, 1195
BsmAI GTCTC 3 cut(s) 641, 845, 1359
BsmFI GGGAC 1 cut(s) 722
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 2 cut(s) 696, 769
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 1399
Bsp143I GATC 6 cut(s) 133, 342, 407, 822, 1151, 1203
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 7 cut(s) 100, 384, 448, 543, 582, 594, 629
BspANI GGCC 2 cut(s) 696, 769
BspCNI CTCAG 4 cut(s) 584, 698, 906, 993
BspFNI CGCG 1 cut(s) 900
BspLI GGNNCC 4 cut(s) 300, 643, 937, 1440
BspOI GCTAGC 1 cut(s) 483
BspPI GGATC 3 cut(s) 415, 830, 1146
BspT107I GGYRCC 2 cut(s) 298, 641
BsrDI GCAATG 3 cut(s) 354, 837, 961
BsrFI RCCGGY 2 cut(s) 386, 428
BsrI ACTGG 2 cut(s) 302, 1200
BssAI RCCGGY 2 cut(s) 386, 428
BssECI CCNNGG 7 cut(s) 24, 294, 363, 966, 1143, 1155, 1399
BssMI GATC 6 cut(s) 133, 342, 407, 822, 1151, 1203
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 1401
Bst4CI ACNGT 3 cut(s) 763, 784, 888
Bst6I CTCTTC 2 cut(s) 68, 84
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 1376
BstC8I GCNNGC 7 cut(s) 100, 144, 388, 430, 481, 1275, 1515
BstDEI CTNAG 8 cut(s) 318, 571, 685, 749, 893, 908, 1001, 1521
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 1 cut(s) 900
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 5 cut(s) 256, 288, 400, 536, 900
BstKTI GATC 6 cut(s) 136, 345, 410, 825, 1154, 1206
BstMAI GTCTC 3 cut(s) 641, 845, 1359
BstMBI GATC 6 cut(s) 133, 342, 407, 822, 1151, 1203
BstMWI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
BstNI CCWGG 1 cut(s) 1401
BstSCI CCNGG 3 cut(s) 216, 1143, 1399
BstUI CGCG 1 cut(s) 900
BstV1I GCAGC 7 cut(s) 107, 603, 626, 638, 781, 1069, 1247
BstX2I RGATCY 1 cut(s) 407
BstXI CCANNNNNNTGG 2 cut(s) 25, 950
BstYI RGATCY 1 cut(s) 407
BsuI GTATCC 2 cut(s) 751, 1415
BsuRI GGCC 2 cut(s) 696, 769
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 1 cut(s) 1086
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 3 cut(s) 18, 309, 615
Cac8I GCNNGC 7 cut(s) 100, 144, 388, 430, 481, 1275, 1515
CaiI CAGNNNCTG 2 cut(s) 794, 1195
CfoI GCGC 5 cut(s) 256, 288, 400, 536, 900
Cfr10I RCCGGY 2 cut(s) 386, 428
Cfr13I GGNCC 3 cut(s) 291, 694, 767
Csp6I GTAC 3 cut(s) 642, 862, 1379
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 4 cut(s) 25, 48, 326, 944
CviQI GTAC 3 cut(s) 642, 862, 1379
DdeI CTNAG 8 cut(s) 318, 571, 685, 749, 893, 908, 1001, 1521
DpnI GATC 6 cut(s) 135, 344, 409, 824, 1153, 1205
DpnII GATC 6 cut(s) 133, 342, 407, 822, 1151, 1203
Eam1104I CTCTTC 2 cut(s) 68, 84
EarI CTCTTC 2 cut(s) 68, 84
Eco130I CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
Eco24I GRGCYC 1 cut(s) 1399
Eco47I GGWCC 1 cut(s) 291
Eco57I CTGAAG 1 cut(s) 1260
Eco72I CACGTG 1 cut(s) 1376
Eco88I CYCGRG 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 1399
EcoT14I CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
EcoT22I ATGCAT 1 cut(s) 1120
EcoT38I GRGCYC 1 cut(s) 1399
ErhI CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
FaeI CATG 4 cut(s) 28, 51, 329, 947
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 1 cut(s) 722
FatI CATG 4 cut(s) 24, 47, 325, 943
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 1501
FbaI TGATCA 1 cut(s) 342
FblI GTMKAC 2 cut(s) 509, 1026
Fnu4HI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
FriOI GRGCYC 1 cut(s) 1399
Fsp4HI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
FspBI CTAG 6 cut(s) 83, 236, 480, 720, 1065, 1340
GlaI GCGC 5 cut(s) 255, 287, 399, 535, 899
GluI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
GsuI CTGGAG 1 cut(s) 122
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 2 cut(s) 696, 769
HapII CCGG 4 cut(s) 218, 387, 429, 1145
HhaI GCGC 5 cut(s) 256, 288, 400, 536, 900
Hin1II CATG 4 cut(s) 28, 51, 329, 947
Hin6I GCGC 5 cut(s) 254, 286, 398, 534, 898
HinP1I GCGC 5 cut(s) 254, 286, 398, 534, 898
HinfI GANTC 3 cut(s) 105, 231, 1285
HpaII CCGG 4 cut(s) 218, 387, 429, 1145
HphI GGTGA 3 cut(s) 157, 251, 1336
Hpy166II GTNNAC 5 cut(s) 510, 1027, 1299, 1379, 1463
Hpy188I TCNGA 6 cut(s) 206, 342, 574, 935, 1002, 1477
Hpy188III TCNNGA 5 cut(s) 248, 422, 806, 1065, 1492
Hpy8I GTNNAC 5 cut(s) 510, 1027, 1299, 1379, 1463
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 4 cut(s) 949, 1120, 1183, 1548
HpyCH4III ACNGT 3 cut(s) 763, 784, 888
HpyCH4IV ACGT 1 cut(s) 1375
HpyF10VI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
HpyF3I CTNAG 8 cut(s) 318, 571, 685, 749, 893, 908, 1001, 1521
HpySE526I ACGT 1 cut(s) 1375
Hsp92II CATG 4 cut(s) 28, 51, 329, 947
HspAI GCGC 5 cut(s) 254, 286, 398, 534, 898
KpnI GGTACC 1 cut(s) 645
KroI GCCGGC 2 cut(s) 386, 428
KroNI GCCGGC 2 cut(s) 388, 430
Ksp22I TGATCA 1 cut(s) 342
Kzo9I GATC 6 cut(s) 133, 342, 407, 822, 1151, 1203
LmnI GCTCC 3 cut(s) 531, 935, 1013
Lsp1109I GCAGC 7 cut(s) 107, 603, 626, 638, 781, 1069, 1247
LweI GCATC 4 cut(s) 241, 415, 781, 799
MaeI CTAG 6 cut(s) 83, 236, 480, 720, 1065, 1340
MaeII ACGT 1 cut(s) 1375
MaeIII GTNAC 4 cut(s) 306, 495, 1324, 1371
MalI GATC 6 cut(s) 135, 344, 409, 824, 1153, 1205
MboI GATC 6 cut(s) 133, 342, 407, 822, 1151, 1203
MboII GAAGA 3 cut(s) 55, 71, 1345
MfeI CAATTG 2 cut(s) 846, 1386
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 1 cut(s) 1399
MlyI GAGTC 2 cut(s) 99, 240
MmeI TCCRAC 1 cut(s) 509
Mph1103I ATGCAT 1 cut(s) 1120
MroNI GCCGGC 2 cut(s) 386, 428
MroXI GAANNNNTTC 1 cut(s) 1550
MseI TTAA 1 cut(s) 1112
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 5 cut(s) 594, 629, 1238, 1418, 1445
MspI CCGG 4 cut(s) 218, 387, 429, 1145
MspR9I CCNGG 3 cut(s) 218, 1145, 1401
MunI CAATTG 2 cut(s) 846, 1386
Mva1269I GAATGC 1 cut(s) 330
MvaI CCWGG 1 cut(s) 1401
MvnI CGCG 1 cut(s) 900
MwoI GCNNNNNNNGC 8 cut(s) 272, 540, 549, 588, 591, 623, 794, 1241
NaeI GCCGGC 2 cut(s) 388, 430
NciI CCSGG 2 cut(s) 218, 1145
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 1501
NdeII GATC 6 cut(s) 133, 342, 407, 822, 1151, 1203
NgoMIV GCCGGC 2 cut(s) 386, 428
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 4 cut(s) 28, 51, 329, 947
NlaIV GGNNCC 4 cut(s) 300, 643, 937, 1440
NmuCI GTSAC 4 cut(s) 306, 495, 1324, 1371
NsiI ATGCAT 1 cut(s) 1120
PaeR7I CTCGAG 1 cut(s) 169
PctI GAATGC 1 cut(s) 330
PdiI GCCGGC 2 cut(s) 388, 430
PdmI GAANNNNTTC 1 cut(s) 1550
PfeI GAWTC 1 cut(s) 1285
PflMI CCANNNNNTGG 1 cut(s) 1195
PkrI GCNGC 8 cut(s) 97, 584, 593, 616, 628, 796, 1084, 1237
PleI GAGTC 2 cut(s) 99, 239
PmaCI CACGTG 1 cut(s) 1376
PmlI CACGTG 1 cut(s) 1376
PpsI GAGTC 2 cut(s) 99, 239
Ppu21I YACGTR 1 cut(s) 1376
Psp6I CCWGG 1 cut(s) 1399
PspCI CACGTG 1 cut(s) 1376
PspGI CCWGG 1 cut(s) 1399
PspN4I GGNNCC 4 cut(s) 300, 643, 937, 1440
PspPI GGNCC 3 cut(s) 291, 694, 767
PspXI VCTCGAGB 1 cut(s) 169
PstNI CAGNNNCTG 2 cut(s) 794, 1195
PsuI RGATCY 1 cut(s) 407
PvuII CAGCTG 3 cut(s) 1238, 1418, 1445
RsaI GTAC 3 cut(s) 643, 863, 1380
RsaNI GTAC 3 cut(s) 642, 862, 1379
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 1 cut(s) 1112
SatI GCNGC 8 cut(s) 96, 583, 592, 615, 627, 795, 1083, 1236
Sau3AI GATC 6 cut(s) 133, 342, 407, 822, 1151, 1203
Sau96I GGNCC 3 cut(s) 291, 694, 767
SchI GAGTC 2 cut(s) 99, 240
ScrFI CCNGG 3 cut(s) 218, 1145, 1401
SduI GDGCHC 1 cut(s) 1399
SfaNI GCATC 4 cut(s) 241, 415, 781, 799
Sfr274I CTCGAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 291
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 3 cut(s) 169, 484, 586
SmoI CTYRAG 3 cut(s) 169, 484, 586
SpeI ACTAGT 1 cut(s) 719
SsiI CCGC 7 cut(s) 100, 384, 448, 543, 582, 594, 629
SspI AATATT 1 cut(s) 188
SspMI CTAG 6 cut(s) 83, 236, 480, 720, 1065, 1340
StyD4I CCNGG 3 cut(s) 216, 1143, 1399
StyI CCWWGG 5 cut(s) 24, 294, 363, 966, 1155
TaaI ACNGT 3 cut(s) 763, 784, 888
TaiI ACGT 1 cut(s) 1378
TaqI TCGA 4 cut(s) 170, 724, 825, 1491
TaqII GACCGA 1 cut(s) 701
TatI WGTACW 2 cut(s) 861, 1378
TauI GCSGC 1 cut(s) 585
TfiI GAWTC 1 cut(s) 1285
Tru1I TTAA 1 cut(s) 1112
Tru9I TTAA 1 cut(s) 1112
TscAI CASTG 3 cut(s) 25, 309, 615
TseFI GTSAC 4 cut(s) 306, 495, 1324, 1371
TseI GCWGC 7 cut(s) 95, 591, 614, 626, 794, 1082, 1235
Tsp45I GTSAC 4 cut(s) 306, 495, 1324, 1371
TspDTI ATGAA 3 cut(s) 389, 1089, 1530
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 3 cut(s) 25, 309, 615
Van91I CCANNNNNTGG 1 cut(s) 1195
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 1 cut(s) 32
XbaI TCTAGA 1 cut(s) 1064
XhoI CTCGAG 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 509, 1026
XmnI GAANNNNTTC 1 cut(s) 1550
XspI CTAG 6 cut(s) 83, 236, 480, 720, 1065, 1340
Zsp2I ATGCAT 1 cut(s) 1120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.