MD05G1117000.v1.1

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000427
Physical Location & Seq
Forward (+)
23808047 .. 23810671
2625 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1117000.v1.1.491

Sequence Viewer

Length: 1731 bp
ATGGCTGCTCTCTCCTCTCTCCTCTCGTTTCTGCTTATAATTGAACCCCAAATCTCAAATGGGTTCGAAACCGAACCACTCAAAACCCCAATCGAAGAATCCACCATTGACGACCTCGAGCTTGCTTTCAAGGTCAAGACTCTGACTTCACGTCAACTGGTTCAGTTCTACCTGGAAAAAATCCAGACCCTCAACGGCCACCTCAGAGGGGTCCTGGAGGTGAACCCAGATGCGCTTTCCCAGGGCGATTCCGCCGACGAACAACGCCGGGAATGGGAATCCAACCCATCAGCCAGAAGCAGACCGCTGCCGAAGCTGCACGGGATTCCGATTCTACTCAAGGACAATATTGGAACCAGGGACAAGCTTAACACCACGGCCGGGTCGTTTGCGCTGTTGGGTTCCAAAGTTCCGAGGGATGCCGGGGTTGTGGCGAAGCTGAGGAGCTCTGGGGCTGTTATTCTAGGAAAGGGTAGCTTGAGCGAGTGGTTGCACATCCGGTCGTTTTCTGCGCCGGACGGATGGAGTGCCAGAGGCGGCCAAGGTGTGAATCCTTATAATCCATCACTGGAAGCTTGTGGATCAAGTAGTGGGCCAGCAATATATGTATCTGCAAATATGGTAGCTGTGGCTCTAGGGGCAGAGACTGACAGCTCTATCTTCTGTCCGTCCAGTTATAACTCGGTAGTAGGCTTCAAGCCAACCGTTGGTCTCACTAGTCGAGCTGGAGTTGTCCCAGTCAGTCCAAGACAGGACACAGTTGGGCCAATCTGTAGGACTGTAGCTGATTCTGTTCATGTCCTGGATGCTATTGTCGGCATTGATAGCAACGATATTGCTACAAATGAAGCCTCACAGTACATTCCGGTGGGTGGCTATGGACAGTTTCTCAGGGCTAATGGTCTCAAAGGAAAGAGATTGGGGATAGTAAGTCGCTTGTTTAGTCAAGTCAAGGACGATCAGTTCTTGAATCAAACTTTTGAGCAGCGCTTTACCACACTGAGGAAACAAGGTGCAGTTTTGGTTGAGAATCTCGACATAGCCAATATTGAAGATATACTGGATTCCTCTTTGAGCGGTGAACTTGTAGCAATATTAGCGGAATTCAAAATGGCCTTGAACCCATACCTAAGTAAACTAGTTGAGTCTCCGGTGAGAAGCTTGAGAGACGTGATAGCATTCAACAAGAATAACTCAGTTTTGGTGAGAAAAGTAAGTGAATATGGACAACATCTCTTAGAAGCGGCTGAGAAGACTAACGGTATAGGAGCAAAAGAGCAAGAAATATTGTCAAATTTAGCAAAATTGTCTAGAGATGGATTTGAGAAGTTGATGAAAGATGAGAGCGTAGATGCCTTGGTAACTTATTCCAACTCTGCTCATTCAATCCTCGGAGTTGGTGGTTTTCCTGGCATTGTTGTGCCGGCAGGATATCATGACACTGAAAAGTACCCATTTGGCATTTGCTTTGGGGGACTAAAGGGTTCGGAGCCAAAGCTGATTGAGGCCGCGTACGCATATGAGCAAGCAACTAAGATCAGGAAGCCACCACCTGTTGGTTCTCAGACCAAATTTATGTCCAACACTGCCAACGTCACTCCTGAAGTTGAGTCCTCGAAATCCACCTTCACCACATCTGAAGAAAGACCCCGCCTAGCCACTCGAGAACACCTCGAACACAGTCCCTTGCAAGAACACAGTCGACGTGAGGGGCTCAATAAGGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

577

Amino Acids

62.27

Weight (kDa)

6.14

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 53 - 455 3.1e-61 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 38, 558, 678
AccB7I CCANNNNNTGG 2 cut(s) 707, 1556
AccBSI CCGCTC 1 cut(s) 1077
AccI GTMKAC 1 cut(s) 1702
AccII CGCG 1 cut(s) 1511
AciI CCGC 8 cut(s) 252, 305, 537, 1077, 1100, 1244, 1509, 1651
AclWI GGATC 1 cut(s) 589
AcoI YGGCCR 3 cut(s) 196, 378, 538
AcsI RAATTY 3 cut(s) 1103, 1294, 1571
AcuI CTGAAG 2 cut(s) 1623, 1659
AfaI GTAC 3 cut(s) 860, 1451, 1514
AfeI AGCGCT 1 cut(s) 989
AfiI CCNNNNNNNGG 7 cut(s) 208, 274, 381, 707, 872, 1002, 1556
AgsI TTSAA 9 cut(s) 44, 130, 697, 970, 1052, 1108, 1120, 1183, 1386
AhdI GACNNNNNGTC 1 cut(s) 150
AhlI ACTAGT 2 cut(s) 716, 1138
AjiI CACGTC 3 cut(s) 152, 1171, 1706
AjnI CCWGG 6 cut(s) 171, 213, 240, 356, 801, 1408
AloI GAACNNNNNNTCC 2 cut(s) 947, 979
Alw21I GWGCWC 1 cut(s) 449
Alw26I GTCTC 5 cut(s) 638, 716, 908, 1152, 1161
AlwI GGATC 1 cut(s) 589
AlwNI CAGNNNCTG 1 cut(s) 647
Ama87I CYCGRG 2 cut(s) 116, 1662
Aor51HI AGCGCT 1 cut(s) 989
AoxI GGCC 7 cut(s) 196, 378, 538, 593, 764, 1113, 1506
ApeKI GCWGC 4 cut(s) 5, 307, 316, 985
ApoI RAATTY 3 cut(s) 1103, 1294, 1571
AspLEI GCGC 4 cut(s) 235, 394, 514, 990
AspS9I GGNCC 3 cut(s) 211, 593, 764
AsuC2I CCSGG 3 cut(s) 269, 382, 424
AsuHPI GGTGA 5 cut(s) 232, 1091, 1165, 1216, 1621
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 2 cut(s) 116, 1662
AvaII GGWCC 1 cut(s) 211
BanII GRGCYC 2 cut(s) 449, 1716
BbsI GAAGAC 1 cut(s) 1259
Bbv12I GWGCWC 1 cut(s) 449
BbvCI CCTCAGC 1 cut(s) 440
BbvI GCAGC 3 cut(s) 294, 303, 997
BccI CCATC 4 cut(s) 295, 516, 571, 1310
BceAI ACGGC 2 cut(s) 211, 393
BciT130I CCWGG 6 cut(s) 173, 215, 242, 358, 803, 1410
BcnI CCSGG 3 cut(s) 269, 382, 424
BcoDI GTCTC 5 cut(s) 638, 716, 908, 1152, 1161
BcuI ACTAGT 2 cut(s) 716, 1138
BfaI CTAG 6 cut(s) 464, 635, 717, 1139, 1311, 1655
BfmI CTRYAG 2 cut(s) 772, 780
BfoI RGCGCY 1 cut(s) 991
BisI GCNGC 7 cut(s) 6, 308, 317, 538, 986, 1245, 1509
BlsI GCNGC 7 cut(s) 7, 309, 318, 539, 987, 1246, 1510
Bme1390I CCNGG 9 cut(s) 173, 215, 242, 269, 358, 382, 424, 803, 1410
Bme18I GGWCC 1 cut(s) 211
BmeRI GACNNNNNGTC 1 cut(s) 150
BmeT110I CYCGRG 2 cut(s) 116, 1662
BmgBI CACGTC 3 cut(s) 152, 1171, 1706
BmgT120I GGNCC 3 cut(s) 211, 593, 764
BmiI GGNNCC 4 cut(s) 212, 355, 403, 1491
BmrFI CCNGG 9 cut(s) 173, 215, 242, 269, 358, 382, 424, 803, 1410
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 5 cut(s) 220, 409, 796, 1342, 1714
BmuI ACTGGG 1 cut(s) 731
BpiI GAAGAC 1 cut(s) 1259
BplI GAGNNNNNCTC 2 cut(s) 1656, 1688
BpmI CTGGAG 2 cut(s) 236, 747
Bpu10I CCTNAGC 1 cut(s) 440
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 3 cut(s) 323, 499, 1183
BpuMI CCSGG 3 cut(s) 269, 382, 424
BsaI GGTCTC 2 cut(s) 716, 908
BsaJI CCNNGG 9 cut(s) 240, 241, 357, 375, 413, 423, 541, 1356, 1390
BsaWI WCCGGW 3 cut(s) 498, 865, 1150
Bsc4I CCNNNNNNNGG 7 cut(s) 208, 274, 381, 707, 872, 1002, 1556
Bse118I RCCGGY 1 cut(s) 1423
Bse1I ACTGG 5 cut(s) 162, 573, 672, 737, 1065
BseBI CCWGG 6 cut(s) 173, 215, 242, 358, 803, 1410
BseDI CCNNGG 9 cut(s) 240, 241, 357, 375, 413, 423, 541, 1356, 1390
BseGI GGATG 5 cut(s) 424, 495, 527, 811, 1729
BseLI CCNNNNNNNGG 7 cut(s) 208, 274, 381, 707, 872, 1002, 1556
BseMII CTCAG 7 cut(s) 217, 431, 904, 992, 1209, 1239, 1577
BseNI ACTGG 5 cut(s) 162, 573, 672, 737, 1065
BseRI GAGGAG 3 cut(s) 4, 11, 457
BseX3I CGGCCG 1 cut(s) 378
BseXI GCAGC 3 cut(s) 294, 303, 997
BsgI GTGCAG 2 cut(s) 302, 1035
Bsh1236I CGCG 1 cut(s) 1511
Bsh1285I CGRYCG 2 cut(s) 381, 503
BshFI GGCC 7 cut(s) 198, 380, 540, 595, 766, 1115, 1508
BsiEI CGRYCG 2 cut(s) 381, 503
BsiHKAI GWGCWC 1 cut(s) 449
BsiHKCI CYCGRG 2 cut(s) 116, 1662
BsiSI CCGG 8 cut(s) 268, 381, 423, 499, 515, 866, 1151, 1424
BsiWI CGTACG 1 cut(s) 1512
BslFI GGGAC 4 cut(s) 374, 719, 1488, 1668
BslI CCNNNNNNNGG 7 cut(s) 208, 274, 381, 707, 872, 1002, 1556
BsmAI GTCTC 5 cut(s) 638, 716, 908, 1152, 1161
BsmBI CGTCTC 1 cut(s) 1161
BsmFI GGGAC 4 cut(s) 374, 719, 1488, 1668
BsmI GAATGC 1 cut(s) 1178
BsnI GGCC 7 cut(s) 198, 380, 540, 595, 766, 1115, 1508
Bso31I GGTCTC 2 cut(s) 716, 908
BsoBI CYCGRG 2 cut(s) 116, 1662
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 2 cut(s) 449, 1716
Bsp143I GATC 3 cut(s) 581, 958, 1536
BspACI CCGC 8 cut(s) 252, 305, 537, 1077, 1100, 1244, 1509, 1651
BspANI GGCC 7 cut(s) 198, 380, 540, 595, 766, 1115, 1508
BspCNI CTCAG 7 cut(s) 216, 432, 903, 993, 1208, 1240, 1576
BspFNI CGCG 1 cut(s) 1511
BspHI TCATGA 1 cut(s) 1435
BspLI GGNNCC 4 cut(s) 212, 355, 403, 1491
BspPI GGATC 1 cut(s) 589
BspT104I TTCGAA 1 cut(s) 66
BspTNI GGTCTC 2 cut(s) 716, 908
BsrBI CCGCTC 1 cut(s) 1077
BsrFI RCCGGY 1 cut(s) 1423
BsrI ACTGG 5 cut(s) 162, 573, 672, 737, 1065
BssAI RCCGGY 1 cut(s) 1423
BssECI CCNNGG 9 cut(s) 240, 241, 357, 375, 413, 423, 541, 1356, 1390
BssMI GATC 3 cut(s) 581, 958, 1536
BssT1I CCWWGG 2 cut(s) 541, 1356
Bst2UI CCWGG 6 cut(s) 173, 215, 242, 358, 803, 1410
Bst4CI ACNGT 8 cut(s) 706, 760, 781, 858, 885, 1262, 1682, 1700
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 4 cut(s) 123, 597, 1425, 1527
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 5 cut(s) 424, 495, 527, 811, 1729
BstFNI CGCG 1 cut(s) 1511
BstH2I RGCGCY 1 cut(s) 991
BstHHI GCGC 4 cut(s) 235, 394, 514, 990
BstKTI GATC 3 cut(s) 584, 961, 1539
BstMAI GTCTC 5 cut(s) 638, 716, 908, 1152, 1161
BstMBI GATC 3 cut(s) 581, 958, 1536
BstMCI CGRYCG 2 cut(s) 381, 503
BstMWI GCNNNNNNNGC 6 cut(s) 313, 316, 638, 825, 1097, 1514
BstNI CCWGG 6 cut(s) 173, 215, 242, 358, 803, 1410
BstSCI CCNGG 9 cut(s) 171, 213, 240, 267, 356, 380, 422, 801, 1408
BstSFI CTRYAG 2 cut(s) 772, 780
BstUI CGCG 1 cut(s) 1511
BstV1I GCAGC 3 cut(s) 294, 303, 997
BstV2I GAAGAC 1 cut(s) 1259
BstZI CGGCCG 1 cut(s) 378
BsuRI GGCC 7 cut(s) 198, 380, 540, 595, 766, 1115, 1508
BtgI CCRYGG 1 cut(s) 375
BtrI CACGTC 3 cut(s) 152, 1171, 1706
BtsCI GGATG 5 cut(s) 424, 495, 527, 811, 1729
BtsI GCAGTG 1 cut(s) 1584
BtsIMutI CAGTG 4 cut(s) 566, 998, 1440, 1584
Cac8I GCNNGC 4 cut(s) 123, 597, 1425, 1527
CaiI CAGNNNCTG 1 cut(s) 647
CciI TCATGA 1 cut(s) 1435
CfoI GCGC 4 cut(s) 235, 394, 514, 990
Cfr10I RCCGGY 1 cut(s) 1423
Cfr13I GGNCC 3 cut(s) 211, 593, 764
Csp6I GTAC 3 cut(s) 859, 1450, 1513
CviAII CATG 2 cut(s) 797, 1436
CviQI GTAC 3 cut(s) 859, 1450, 1513
DpnI GATC 3 cut(s) 583, 960, 1538
DpnII GATC 3 cut(s) 581, 958, 1536
DriI GACNNNNNGTC 1 cut(s) 150
EaeI YGGCCR 3 cut(s) 196, 378, 538
EagI CGGCCG 1 cut(s) 378
Eam1105I GACNNNNNGTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 241
Ecl136II GAGCTC 1 cut(s) 447
EclXI CGGCCG 1 cut(s) 378
Eco130I CCWWGG 2 cut(s) 541, 1356
Eco24I GRGCYC 2 cut(s) 449, 1716
Eco31I GGTCTC 2 cut(s) 716, 908
Eco32I GATATC 1 cut(s) 1433
Eco47I GGWCC 1 cut(s) 211
Eco47III AGCGCT 1 cut(s) 989
Eco52I CGGCCG 1 cut(s) 378
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 2 cut(s) 1623, 1659
Eco88I CYCGRG 2 cut(s) 116, 1662
EcoICRI GAGCTC 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 1103
EcoRII CCWGG 6 cut(s) 171, 213, 240, 356, 801, 1408
EcoRV GATATC 1 cut(s) 1433
EcoT14I CCWWGG 2 cut(s) 541, 1356
EcoT38I GRGCYC 2 cut(s) 449, 1716
ErhI CCWWGG 2 cut(s) 541, 1356
Esp3I CGTCTC 1 cut(s) 1161
FaeI CATG 2 cut(s) 800, 1439
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 4 cut(s) 374, 719, 1488, 1668
FatI CATG 2 cut(s) 796, 1435
FauI CCCGC 1 cut(s) 1658
FauNDI CATATG 1 cut(s) 1519
FblI GTMKAC 1 cut(s) 1702
Fnu4HI GCNGC 7 cut(s) 6, 308, 317, 538, 986, 1245, 1509
FokI GGATG 4 cut(s) 431, 482, 534, 818
FriOI GRGCYC 2 cut(s) 449, 1716
Fsp4HI GCNGC 7 cut(s) 6, 308, 317, 538, 986, 1245, 1509
FspBI CTAG 6 cut(s) 464, 635, 717, 1139, 1311, 1655
GlaI GCGC 4 cut(s) 234, 393, 513, 989
GluI GCNGC 7 cut(s) 6, 308, 317, 538, 986, 1245, 1509
GsuI CTGGAG 2 cut(s) 236, 747
HaeII RGCGCY 1 cut(s) 991
HaeIII GGCC 7 cut(s) 198, 380, 540, 595, 766, 1115, 1508
HapII CCGG 8 cut(s) 268, 381, 423, 499, 515, 866, 1151, 1424
HhaI GCGC 4 cut(s) 235, 394, 514, 990
Hin1II CATG 2 cut(s) 800, 1439
Hin6I GCGC 4 cut(s) 233, 392, 512, 988
HinP1I GCGC 4 cut(s) 233, 392, 512, 988
HincII GTYRAC 2 cut(s) 155, 1703
HindII GTYRAC 2 cut(s) 155, 1703
HindIII AAGCTT 3 cut(s) 365, 573, 1159
HpaII CCGG 8 cut(s) 268, 381, 423, 499, 515, 866, 1151, 1424
HphI GGTGA 5 cut(s) 232, 1091, 1165, 1216, 1621
Hpy166II GTNNAC 5 cut(s) 155, 223, 1082, 1136, 1703
Hpy188I TCNGA 8 cut(s) 144, 206, 330, 414, 1394, 1489, 1566, 1639
Hpy188III TCNNGA 9 cut(s) 136, 184, 967, 1034, 1311, 1436, 1540, 1601, 1664
Hpy8I GTNNAC 5 cut(s) 155, 223, 1082, 1136, 1703
Hpy99I CGWCG 2 cut(s) 260, 1707
HpyAV CCTTC 1 cut(s) 1636
HpyCH4III ACNGT 8 cut(s) 706, 760, 781, 858, 885, 1262, 1682, 1700
HpyCH4IV ACGT 4 cut(s) 151, 1170, 1593, 1705
HpyCH4V TGCA 5 cut(s) 319, 493, 614, 1016, 1690
HpyF10VI GCNNNNNNNGC 6 cut(s) 313, 316, 638, 825, 1097, 1514
HpySE526I ACGT 4 cut(s) 151, 1170, 1593, 1705
Hsp92II CATG 2 cut(s) 800, 1439
HspAI GCGC 4 cut(s) 233, 392, 512, 988
KroI GCCGGC 1 cut(s) 1423
KroNI GCCGGC 1 cut(s) 1425
Kzo9I GATC 3 cut(s) 581, 958, 1536
LmnI GCTCC 3 cut(s) 444, 1268, 1489
Lsp1109I GCAGC 3 cut(s) 294, 303, 997
LweI GCATC 5 cut(s) 220, 409, 796, 1342, 1714
MaeI CTAG 6 cut(s) 464, 635, 717, 1139, 1311, 1655
MaeII ACGT 4 cut(s) 151, 1170, 1593, 1705
MaeIII GTNAC 2 cut(s) 1360, 1594
MalI GATC 3 cut(s) 583, 960, 1538
MbiI CCGCTC 1 cut(s) 1077
MboI GATC 3 cut(s) 581, 958, 1536
MboII GAAGA 5 cut(s) 107, 652, 1064, 1264, 1652
MhlI GDGCHC 2 cut(s) 449, 1716
MluCI AATT 5 cut(s) 39, 1103, 1294, 1304, 1571
MlyI GAGTC 3 cut(s) 133, 1154, 1619
MmeI TCCRAC 3 cut(s) 306, 1395, 1605
MroNI GCCGGC 1 cut(s) 1423
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 2 cut(s) 866, 1418
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 8 cut(s) 268, 381, 423, 499, 515, 866, 1151, 1424
MspR9I CCNGG 9 cut(s) 173, 215, 242, 269, 358, 382, 424, 803, 1410
Mva1269I GAATGC 1 cut(s) 1178
MvaI CCWGG 6 cut(s) 173, 215, 242, 358, 803, 1410
MvnI CGCG 1 cut(s) 1511
MwoI GCNNNNNNNGC 6 cut(s) 313, 316, 638, 825, 1097, 1514
NaeI GCCGGC 1 cut(s) 1425
NciI CCSGG 3 cut(s) 269, 382, 424
NdeI CATATG 1 cut(s) 1519
NdeII GATC 3 cut(s) 581, 958, 1536
NgoMIV GCCGGC 1 cut(s) 1423
NlaIII CATG 2 cut(s) 800, 1439
NlaIV GGNNCC 4 cut(s) 212, 355, 403, 1491
NmuCI GTSAC 1 cut(s) 1594
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 2 cut(s) 116, 1662
PagI TCATGA 1 cut(s) 1435
PasI CCCWGGG 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PctI GAATGC 1 cut(s) 1178
PdiI GCCGGC 1 cut(s) 1425
Pfl23II CGTACG 1 cut(s) 1512
PflMI CCANNNNNTGG 2 cut(s) 707, 1556
PfoI TCCNGGA 2 cut(s) 213, 801
PkrI GCNGC 7 cut(s) 7, 309, 318, 539, 987, 1246, 1510
PleI GAGTC 3 cut(s) 133, 1153, 1618
PpsI GAGTC 3 cut(s) 133, 1153, 1618
PpuMI RGGWCCY 1 cut(s) 211
PsiI TTATAA 3 cut(s) 38, 558, 678
Psp124BI GAGCTC 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 6 cut(s) 171, 213, 240, 356, 801, 1408
PspGI CCWGG 6 cut(s) 171, 213, 240, 356, 801, 1408
PspLI CGTACG 1 cut(s) 1512
PspN4I GGNNCC 4 cut(s) 212, 355, 403, 1491
PspPI GGNCC 3 cut(s) 211, 593, 764
PspPPI RGGWCCY 1 cut(s) 211
PspXI VCTCGAGB 1 cut(s) 116
PstNI CAGNNNCTG 1 cut(s) 647
RsaI GTAC 3 cut(s) 860, 1451, 1514
RsaNI GTAC 3 cut(s) 859, 1450, 1513
RseI CAYNNNNRTG 2 cut(s) 866, 1418
SacI GAGCTC 1 cut(s) 449
SalI GTCGAC 1 cut(s) 1701
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 7 cut(s) 6, 308, 317, 538, 986, 1245, 1509
Sau3AI GATC 3 cut(s) 581, 958, 1536
Sau96I GGNCC 3 cut(s) 211, 593, 764
SchI GAGTC 3 cut(s) 133, 1154, 1619
ScrFI CCNGG 9 cut(s) 173, 215, 242, 269, 358, 382, 424, 803, 1410
SduI GDGCHC 2 cut(s) 449, 1716
SfaNI GCATC 5 cut(s) 220, 409, 796, 1342, 1714
SfcI CTRYAG 2 cut(s) 772, 780
Sfr274I CTCGAG 2 cut(s) 116, 1662
SfuI TTCGAA 1 cut(s) 66
SinI GGWCC 1 cut(s) 211
SlaI CTCGAG 2 cut(s) 116, 1662
SmiMI CAYNNNNRTG 2 cut(s) 866, 1418
SmlI CTYRAG 5 cut(s) 116, 338, 478, 1162, 1662
SmoI CTYRAG 5 cut(s) 116, 338, 478, 1162, 1662
SpeI ACTAGT 2 cut(s) 716, 1138
Sse9I AATT 5 cut(s) 39, 1103, 1294, 1304, 1571
SsiI CCGC 8 cut(s) 252, 305, 537, 1077, 1100, 1244, 1509, 1651
SspI AATATT 4 cut(s) 349, 1048, 1095, 1287
SspMI CTAG 6 cut(s) 464, 635, 717, 1139, 1311, 1655
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 9 cut(s) 171, 213, 240, 267, 356, 380, 422, 801, 1408
StyI CCWWGG 2 cut(s) 541, 1356
TaaI ACNGT 8 cut(s) 706, 760, 781, 858, 885, 1262, 1682, 1700
TaiI ACGT 4 cut(s) 154, 1173, 1596, 1708
TaqI TCGA 9 cut(s) 66, 93, 117, 721, 1035, 1616, 1663, 1674, 1702
TasI AATT 5 cut(s) 39, 1103, 1294, 1304, 1571
TatI WGTACW 1 cut(s) 858
TauI GCSGC 3 cut(s) 540, 1247, 1511
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 4 cut(s) 573, 1005, 1447, 1591
TseFI GTSAC 1 cut(s) 1594
TseI GCWGC 4 cut(s) 5, 307, 316, 985
Tsp45I GTSAC 1 cut(s) 1594
TspDTI ATGAA 3 cut(s) 785, 861, 1349
TspGWI ACGGA 2 cut(s) 534, 657
TspRI CASTG 4 cut(s) 573, 1005, 1447, 1591
Van91I CCANNNNNTGG 2 cut(s) 707, 1556
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 3 cut(s) 1103, 1294, 1571
XbaI TCTAGA 1 cut(s) 1310
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XhoI CTCGAG 2 cut(s) 116, 1662
XmiI GTMKAC 1 cut(s) 1702
XspI CTAG 6 cut(s) 464, 635, 717, 1139, 1311, 1655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.