Rw6G000020

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000427
Physical Location & Seq
Forward (+)
66624 .. 69207
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000020.1

Sequence Viewer

Length: 1332 bp
ATGCTTCATAGAGTGATAGATGTGAACCCGGATGCACTTTACCAAGCTGACAAGGCTGACTATCAGCGAAAGGCTAAGGCCCCTGGTTATTATTCCGGCTTGCATGGCATTCCTGTTTTGCTGAAGGATAACATTGGAACCAGAGATAAGCTGAACACTACTGCTGGCTCTTTTGCGCTGCTCGGCTCTGTCGTGCCCCGGGATGCTGGCGTGGTGACCGAGTTGAGGAGCGCTGGAGCCATTATCTTGGGGAAGGCTAGCTTGAGTGAGTGGGCTCAGGTCAGGTCTCTTACGTCACCCCCAGGTTTCAGCCCCAGAGGTGGCCAAGGAAAGGTAAGCATGACACTTGTCAAATGCTTAACCCTTATGTACTATCTGCAAGTCCATGTGGATCAAGCAGTGGATCATCAATATATATCAGTTGCAGCAAATTTGGTAGCTGTATCTCTTGGTACTGAGACCGATGGCTCAATACTATGTCCAGCAAGCTTTAACTCAGTAGTCGGCATCAAACCAACCGTTGGCCTCACAAGCCGAGCCGGGGTGATCCCAGTTACTCCCAGACAGGACACCATTGGACCCATCTGCAGGACTGTGACAGATGCTGTTTATGTTCTTGATGCGATTGTAGGCTATGACTACAAGGATCAAGCAACCAAAGAAGCATCGAAATACATTCCACGCGGTGGGTATAAGCAGTTCCTTGGGGCTTATGGGCTCAAGGGAAAGAGACTGGGAATAGTGAGGAATCCCTTCTTCACCTCTGGGAGTGGATCTCTCCAAATTCAAGCATTTGAGCATCATTTCCAAACGCTAAGGCAAGGAGGTGCTGTTTTGGTAGACCATCTGGAAATAGCAAACATTGATACTATCTTGAATTTTAATTTGAGTGGAGAAGCAATAGCAACAGTGGCTGAGTTCAAACTAGCCATAAATGCATATCTAGAAGACCTTGTGGTGTCTCCAGTTAGATCTTTGGCAGATATCATAGCCTTCAACTTGAAGTTTTCTGGTGTGGAAATGATCAAGGAATTTGGCCAAGACATTTTTCTTGCAGCACAAGCCACAAATGGGATAGGCAACAATGAGAAGGCAGCATTGTTAAATTTAGCAAAGGTGACAAAAGATGGATTTGAGAAACTAATGACATACAACAGGCTAGATGCATTGGTGAGTGGTGACTCCCGTGTCCCTGCTGGATATGACAGGAAGGGTGTGCCTTTCGGCATAACTTTTGGGGGTCTAAAGGGATCAGAACCAAAGCTTATACAGATTGCCGACGGTTTCGGGCAAGCTACTAAGATTAGAAAACCTCCCACATTTCTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

443

Amino Acids

47.47

Weight (kDa)

9.06

Isoelectric Point (pI)

22.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 9 - 99 5.2e-19 Amidase
Amidase PF01425 141 - 305 4e-30 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1187
AccB7I CCANNNNNTGG 2 cut(s) 521, 686
AccI GTMKAC 1 cut(s) 840
AccII CGCG 1 cut(s) 684
AciI CCGC 1 cut(s) 684
AclWI GGATC 6 cut(s) 399, 411, 541, 654, 781, 1258
AcoI YGGCCR 2 cut(s) 322, 1036
AcsI RAATTY 5 cut(s) 430, 783, 877, 1031, 1105
AcuI CTGAAG 1 cut(s) 143
AdeI CACNNNGTG 1 cut(s) 686
AfaI GTAC 2 cut(s) 371, 454
AfeI AGCGCT 1 cut(s) 232
AfiI CCNNNNNNNGG 8 cut(s) 225, 320, 331, 521, 541, 588, 686, 1071
AgsI TTSAA 5 cut(s) 788, 877, 922, 997, 1003
AjnI CCWGG 2 cut(s) 82, 301
AjuI GAANNNNNNNTTGG 2 cut(s) 1032, 1064
AluBI AGCT 7 cut(s) 47, 151, 261, 440, 489, 1264, 1295
AluI AGCT 7 cut(s) 47, 151, 261, 440, 489, 1264, 1295
Alw26I GTCTC 4 cut(s) 291, 452, 724, 966
AlwI GGATC 6 cut(s) 399, 411, 541, 654, 781, 1258
AlwNI CAGNNNCTG 2 cut(s) 605, 914
Ama87I CYCGRG 1 cut(s) 198
Aor51HI AGCGCT 1 cut(s) 232
AoxI GGCC 4 cut(s) 78, 322, 523, 1036
ApeKI GCWGC 4 cut(s) 178, 425, 1055, 1094
ApoI RAATTY 5 cut(s) 430, 783, 877, 1031, 1105
Asp700I GAANNNNTTC 1 cut(s) 752
AspLEI GCGC 2 cut(s) 178, 233
AspS9I GGNCC 2 cut(s) 79, 578
AsuC2I CCSGG 4 cut(s) 29, 199, 200, 541
AsuHPI GGTGA 7 cut(s) 226, 288, 556, 751, 1129, 1183, 1190
AsuNHI GCTAGC 1 cut(s) 257
AvaI CYCGRG 1 cut(s) 198
AvaII GGWCC 1 cut(s) 578
BaeGI GKGCMC 1 cut(s) 198
BalI TGGCCA 2 cut(s) 324, 1038
BanII GRGCYC 2 cut(s) 277, 720
BbsI GAAGAC 1 cut(s) 954
BbvI GCAGC 4 cut(s) 165, 437, 1067, 1106
BccI CCATC 4 cut(s) 458, 590, 852, 1121
BciT130I CCWGG 2 cut(s) 84, 303
BclI TGATCA 1 cut(s) 1023
BcnI CCSGG 4 cut(s) 29, 199, 200, 541
BcoDI GTCTC 4 cut(s) 291, 452, 724, 966
BfaI CTAG 4 cut(s) 258, 926, 944, 1160
BfmI CTRYAG 1 cut(s) 586
BfoI RGCGCY 1 cut(s) 234
BglII AGATCT 1 cut(s) 971
BisI GCNGC 4 cut(s) 179, 426, 1056, 1095
BlsI GCNGC 4 cut(s) 180, 427, 1057, 1096
Bme1390I CCNGG 6 cut(s) 29, 84, 199, 200, 303, 541
Bme18I GGWCC 1 cut(s) 578
BmeT110I CYCGRG 1 cut(s) 198
BmgT120I GGNCC 2 cut(s) 79, 578
BmiI GGNNCC 4 cut(s) 81, 139, 238, 580
BmrFI CCNGG 6 cut(s) 29, 84, 199, 200, 303, 541
BmrI ACTGGG 2 cut(s) 545, 743
BmsI GCATC 8 cut(s) 22, 193, 516, 592, 610, 674, 808, 1153
BmtI GCTAGC 1 cut(s) 261
BmuI ACTGGG 2 cut(s) 545, 743
BoxI GACNNNNGTC 1 cut(s) 347
BpiI GAAGAC 1 cut(s) 954
BplI GAGNNNNNCTC 2 cut(s) 760, 792
BpmI CTGGAG 2 cut(s) 255, 948
Bpu10I CCTNAGC 3 cut(s) 75, 276, 815
BpuEI CTTGAG 2 cut(s) 283, 704
BpuMI CCSGG 4 cut(s) 29, 199, 200, 541
BsaI GGTCTC 2 cut(s) 291, 452
BsaJI CCNNGG 7 cut(s) 82, 197, 198, 301, 325, 540, 703
BsaXI ACNNNNNCTCC 2 cut(s) 816, 846
Bsc4I CCNNNNNNNGG 8 cut(s) 225, 320, 331, 521, 541, 588, 686, 1071
Bse1I ACTGG 3 cut(s) 551, 738, 965
BseBI CCWGG 2 cut(s) 84, 303
BseDI CCNNGG 7 cut(s) 82, 197, 198, 301, 325, 540, 703
BseGI GGATG 2 cut(s) 37, 208
BseLI CCNNNNNNNGG 8 cut(s) 225, 320, 331, 521, 541, 588, 686, 1071
BseMII CTCAG 4 cut(s) 290, 447, 510, 906
BseNI ACTGG 3 cut(s) 551, 738, 965
BseRI GAGGAG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 198
BseXI GCAGC 4 cut(s) 165, 437, 1067, 1106
Bsh1236I CGCG 1 cut(s) 684
BshFI GGCC 4 cut(s) 80, 324, 525, 1038
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 4 cut(s) 29, 96, 199, 540
BslFI GGGAC 1 cut(s) 1175
BslI CCNNNNNNNGG 8 cut(s) 225, 320, 331, 521, 541, 588, 686, 1071
BsmAI GTCTC 4 cut(s) 291, 452, 724, 966
BsmFI GGGAC 1 cut(s) 1175
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 4 cut(s) 80, 324, 525, 1038
Bso31I GGTCTC 2 cut(s) 291, 452
BsoBI CYCGRG 1 cut(s) 198
Bsp1286I GDGCHC 3 cut(s) 198, 277, 720
Bsp143I GATC 8 cut(s) 391, 403, 546, 646, 773, 971, 1023, 1250
BspACI CCGC 1 cut(s) 684
BspANI GGCC 4 cut(s) 80, 324, 525, 1038
BspCNI CTCAG 4 cut(s) 289, 448, 509, 907
BspFNI CGCG 1 cut(s) 684
BspLI GGNNCC 4 cut(s) 81, 139, 238, 580
BspMAI CTGCAG 1 cut(s) 590
BspOI GCTAGC 1 cut(s) 261
BspPI GGATC 6 cut(s) 399, 411, 541, 654, 781, 1258
BspTNI GGTCTC 2 cut(s) 291, 452
BsrI ACTGG 3 cut(s) 551, 738, 965
BssECI CCNNGG 7 cut(s) 82, 197, 198, 301, 325, 540, 703
BssMI GATC 8 cut(s) 391, 403, 546, 646, 773, 971, 1023, 1250
BssT1I CCWWGG 2 cut(s) 325, 703
Bst2UI CCWGG 2 cut(s) 84, 303
Bst4CI ACNGT 4 cut(s) 520, 595, 910, 1283
BstC8I GCNNGC 6 cut(s) 101, 166, 208, 259, 487, 1293
BstDEI CTNAG 7 cut(s) 75, 276, 456, 496, 815, 915, 1299
BstEII GGTNACC 1 cut(s) 214
BstF5I GGATG 2 cut(s) 37, 208
BstFNI CGCG 1 cut(s) 684
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 2 cut(s) 178, 233
BstKTI GATC 8 cut(s) 394, 406, 549, 649, 776, 974, 1026, 1253
BstMAI GTCTC 4 cut(s) 291, 452, 724, 966
BstMBI GATC 8 cut(s) 391, 403, 546, 646, 773, 971, 1023, 1250
BstMWI GCNNNNNNNGC 6 cut(s) 53, 105, 531, 911, 935, 1061
BstNI CCWGG 2 cut(s) 84, 303
BstPAI GACNNNNGTC 1 cut(s) 347
BstPI GGTNACC 1 cut(s) 214
BstSCI CCNGG 6 cut(s) 27, 82, 197, 198, 301, 539
BstSFI CTRYAG 1 cut(s) 586
BstSLI GKGCMC 1 cut(s) 198
BstUI CGCG 1 cut(s) 684
BstV1I GCAGC 4 cut(s) 165, 437, 1067, 1106
BstV2I GAAGAC 1 cut(s) 954
BstX2I RGATCY 2 cut(s) 773, 971
BstXI CCANNNNNNTGG 1 cut(s) 247
BstYI RGATCY 2 cut(s) 773, 971
BsuRI GGCC 4 cut(s) 80, 324, 525, 1038
BtsCI GGATG 2 cut(s) 37, 208
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 405, 915
Cac8I GCNNGC 6 cut(s) 101, 166, 208, 259, 487, 1293
CaiI CAGNNNCTG 2 cut(s) 605, 914
CfoI GCGC 2 cut(s) 178, 233
Cfr13I GGNCC 2 cut(s) 79, 578
Cfr9I CCCGGG 1 cut(s) 198
Csp6I GTAC 2 cut(s) 370, 453
CviAII CATG 3 cut(s) 104, 340, 386
CviQI GTAC 2 cut(s) 370, 453
DdeI CTNAG 7 cut(s) 75, 276, 456, 496, 815, 915, 1299
DpnI GATC 8 cut(s) 393, 405, 548, 648, 775, 973, 1025, 1252
DpnII GATC 8 cut(s) 391, 403, 546, 646, 773, 971, 1023, 1250
DraIII CACNNNGTG 1 cut(s) 686
DrdI GACNNNNNNGTC 1 cut(s) 1187
DseDI GACNNNNNNGTC 1 cut(s) 1187
EaeI YGGCCR 2 cut(s) 322, 1036
Eco130I CCWWGG 2 cut(s) 325, 703
Eco24I GRGCYC 2 cut(s) 277, 720
Eco31I GGTCTC 2 cut(s) 291, 452
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 1 cut(s) 578
Eco47III AGCGCT 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 198
Eco91I GGTNACC 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 79
EcoO65I GGTNACC 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 82, 301
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 2 cut(s) 325, 703
EcoT22I ATGCAT 2 cut(s) 940, 1168
EcoT38I GRGCYC 2 cut(s) 277, 720
ErhI CCWWGG 2 cut(s) 325, 703
FaeI CATG 3 cut(s) 107, 343, 389
FalI AAGNNNNNCTT 2 cut(s) 245, 277
FaqI GGGAC 1 cut(s) 1175
FatI CATG 3 cut(s) 103, 339, 385
FbaI TGATCA 1 cut(s) 1023
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 4 cut(s) 179, 426, 1056, 1095
FokI GGATG 2 cut(s) 44, 215
FriOI GRGCYC 2 cut(s) 277, 720
Fsp4HI GCNGC 4 cut(s) 179, 426, 1056, 1095
FspBI CTAG 4 cut(s) 258, 926, 944, 1160
GlaI GCGC 2 cut(s) 177, 232
GluI GCNGC 4 cut(s) 179, 426, 1056, 1095
GsuI CTGGAG 2 cut(s) 255, 948
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 4 cut(s) 80, 324, 525, 1038
HapII CCGG 4 cut(s) 29, 96, 199, 540
HhaI GCGC 2 cut(s) 178, 233
Hin1II CATG 3 cut(s) 107, 343, 389
Hin6I GCGC 2 cut(s) 176, 231
HinP1I GCGC 2 cut(s) 176, 231
HindIII AAGCTT 2 cut(s) 487, 1262
HinfI GANTC 2 cut(s) 748, 1181
HpaII CCGG 4 cut(s) 29, 96, 199, 540
HphI GGTGA 7 cut(s) 226, 288, 556, 751, 1129, 1183, 1190
Hpy166II GTNNAC 2 cut(s) 25, 841
Hpy188I TCNGA 1 cut(s) 1255
Hpy188III TCNNGA 4 cut(s) 617, 848, 874, 944
Hpy8I GTNNAC 2 cut(s) 25, 841
Hpy99I CGWCG 1 cut(s) 1283
HpyAV CCTTC 6 cut(s) 118, 247, 763, 1003, 1084, 1204
HpyCH4III ACNGT 4 cut(s) 520, 595, 910, 1283
HpyCH4IV ACGT 1 cut(s) 293
HpyCH4V TGCA 8 cut(s) 35, 103, 379, 425, 588, 938, 1055, 1166
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 105, 531, 911, 935, 1061
HpyF3I CTNAG 7 cut(s) 75, 276, 456, 496, 815, 915, 1299
HpySE526I ACGT 1 cut(s) 293
Hsp92II CATG 3 cut(s) 107, 343, 389
HspAI GCGC 2 cut(s) 176, 231
Ksp22I TGATCA 1 cut(s) 1023
Kzo9I GATC 8 cut(s) 391, 403, 546, 646, 773, 971, 1023, 1250
LmnI GCTCC 2 cut(s) 228, 236
Lsp1109I GCAGC 4 cut(s) 165, 437, 1067, 1106
LweI GCATC 8 cut(s) 22, 193, 516, 592, 610, 674, 808, 1153
MaeI CTAG 4 cut(s) 258, 926, 944, 1160
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 6 cut(s) 214, 294, 553, 595, 1117, 1178
MalI GATC 8 cut(s) 393, 405, 548, 648, 775, 973, 1025, 1252
MboI GATC 8 cut(s) 391, 403, 546, 646, 773, 971, 1023, 1250
MboII GAAGA 2 cut(s) 748, 959
MflI RGATCY 2 cut(s) 773, 971
MhlI GDGCHC 3 cut(s) 198, 277, 720
MlsI TGGCCA 2 cut(s) 324, 1038
MluCI AATT 6 cut(s) 430, 783, 877, 883, 1031, 1105
MluNI TGGCCA 2 cut(s) 324, 1038
MlyI GAGTC 1 cut(s) 1175
MnlI CCTC 7 cut(s) 219, 311, 536, 738, 772, 818, 1323
Mox20I TGGCCA 2 cut(s) 324, 1038
Mph1103I ATGCAT 2 cut(s) 940, 1168
MroXI GAANNNNTTC 1 cut(s) 752
MscI TGGCCA 2 cut(s) 324, 1038
MseI TTAA 4 cut(s) 359, 492, 882, 1103
Msp20I TGGCCA 2 cut(s) 324, 1038
MspI CCGG 4 cut(s) 29, 96, 199, 540
MspR9I CCNGG 6 cut(s) 29, 84, 199, 200, 303, 541
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 2 cut(s) 84, 303
MvnI CGCG 1 cut(s) 684
MwoI GCNNNNNNNGC 6 cut(s) 53, 105, 531, 911, 935, 1061
NciI CCSGG 4 cut(s) 29, 199, 200, 541
NdeII GATC 8 cut(s) 391, 403, 546, 646, 773, 971, 1023, 1250
NheI GCTAGC 1 cut(s) 257
NlaIII CATG 3 cut(s) 107, 343, 389
NlaIV GGNNCC 4 cut(s) 81, 139, 238, 580
NmeAIII GCCGAG 2 cut(s) 162, 560
NmuCI GTSAC 5 cut(s) 214, 294, 595, 1117, 1178
NsiI ATGCAT 2 cut(s) 940, 1168
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 752
PfeI GAWTC 1 cut(s) 748
PflMI CCANNNNNTGG 2 cut(s) 521, 686
PkrI GCNGC 4 cut(s) 180, 427, 1057, 1096
PleI GAGTC 1 cut(s) 1175
PpsI GAGTC 1 cut(s) 1175
PshAI GACNNNNGTC 1 cut(s) 347
Psp6I CCWGG 2 cut(s) 82, 301
PspEI GGTNACC 1 cut(s) 214
PspGI CCWGG 2 cut(s) 82, 301
PspN4I GGNNCC 4 cut(s) 81, 139, 238, 580
PspPI GGNCC 2 cut(s) 79, 578
PsrI GAACNNNNNNTAC 2 cut(s) 683, 715
PstI CTGCAG 1 cut(s) 590
PstNI CAGNNNCTG 2 cut(s) 605, 914
PsuI RGATCY 2 cut(s) 773, 971
RsaI GTAC 2 cut(s) 371, 454
RsaNI GTAC 2 cut(s) 370, 453
SaqAI TTAA 4 cut(s) 359, 492, 882, 1103
SatI GCNGC 4 cut(s) 179, 426, 1056, 1095
Sau3AI GATC 8 cut(s) 391, 403, 546, 646, 773, 971, 1023, 1250
Sau96I GGNCC 2 cut(s) 79, 578
SchI GAGTC 1 cut(s) 1175
ScrFI CCNGG 6 cut(s) 29, 84, 199, 200, 303, 541
SduI GDGCHC 3 cut(s) 198, 277, 720
SfaNI GCATC 8 cut(s) 22, 193, 516, 592, 610, 674, 808, 1153
SfcI CTRYAG 1 cut(s) 586
SinI GGWCC 1 cut(s) 578
SmaI CCCGGG 1 cut(s) 200
SmlI CTYRAG 2 cut(s) 262, 719
SmoI CTYRAG 2 cut(s) 262, 719
Sse9I AATT 6 cut(s) 430, 783, 877, 883, 1031, 1105
SsiI CCGC 1 cut(s) 684
SspMI CTAG 4 cut(s) 258, 926, 944, 1160
StyD4I CCNGG 6 cut(s) 27, 82, 197, 198, 301, 539
StyI CCWWGG 2 cut(s) 325, 703
TaaI ACNGT 4 cut(s) 520, 595, 910, 1283
TaiI ACGT 1 cut(s) 296
TaqI TCGA 1 cut(s) 668
TaqII GACCGA 2 cut(s) 233, 476
TasI AATT 6 cut(s) 430, 783, 877, 883, 1031, 1105
TatI WGTACW 1 cut(s) 369
TfiI GAWTC 1 cut(s) 748
Tru1I TTAA 4 cut(s) 359, 492, 882, 1103
Tru9I TTAA 4 cut(s) 359, 492, 882, 1103
TscAI CASTG 2 cut(s) 405, 915
TseFI GTSAC 5 cut(s) 214, 294, 595, 1117, 1178
TseI GCWGC 4 cut(s) 178, 425, 1055, 1094
Tsp45I GTSAC 5 cut(s) 214, 294, 595, 1117, 1178
TspMI CCCGGG 1 cut(s) 198
TspRI CASTG 2 cut(s) 405, 915
Van91I CCANNNNNTGG 2 cut(s) 521, 686
VpaK11BI GGWCC 1 cut(s) 578
XapI RAATTY 5 cut(s) 430, 783, 877, 1031, 1105
XbaI TCTAGA 1 cut(s) 943
XmaI CCCGGG 1 cut(s) 198
XmiI GTMKAC 1 cut(s) 840
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 4 cut(s) 258, 926, 944, 1160
Zsp2I ATGCAT 2 cut(s) 940, 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.