Rroxscaffold_7G00215640

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000427
Physical Location & Seq
Forward (+)
66224714 .. 66227011
2298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215640.1

Sequence Viewer

Length: 1425 bp
ATGGCTACAAAACCACCATCATGCTTTTCTCTCTTCTCAGTATTGGTGTTGATTCTTCTAGCCTTAACATCATTTGGGTCCCAAAGTTTCACTGTCCATGGGTTGTCAATTAGAGAAGCTACAATCTACGATCTTCAACTGGCTTTCAAACAGAACCAACTAACTTCCAGACAAGTAGTTCAGTTCTACCTTCAAGAAATCCGCAGGCTCAACCCGGTTCTTAACGGTGTCATAGAGGTGAACCCGGATGCACTTTACCAAGCTGACAAGGCTGACTATCAGCGAAAGGCTAAGGCCCCTGGTTATTATTCCGGCTTGCATGGCATTCCTGTTTTGCTCAAGGATAACATTGGAACCAGAGATAAGCTGAACACTACTGCTGGCTCTTTAGCGCTGCTCGGCTCTGTTGTGCCCCGGGATGCTGGCGTGGTGACCAAGTTGAGGAGCGCTGGAGCCATTATCTTGGGGAAGGCTAGCTTGAGTGAGTGGGCTCAGGTCAGGTCTCTTACGTCACCCCCAGGTTTCAGCCCCAGAGGTGGCCAAGGAAAGAACCCTTATGTACTATCTGCAAGTCCATGTGGATCAAGCAGTGGATCATCAATATCAGTTGCAGCAAATTTGGTAGCTGTATCTCTTGGTACTGAGACCGATGGCTCAATACTATGTCCAGCAAGCTTTGACTCAGTAGTCGGCATCAAACCAACCGTTGGACTCACAAGCCGAGCCGGGGTGATACCAGTTACTCCCAGACAGGACACCATTGGACCCATCTGCAGGACTATGACAGATGCTGTTTATGTTCTTGATGCGATTTTCCTTGGGGCTTATGGGCTCAAGGGAAAGAGAATGGGAATAGTGAGGAATCCCTTCTTCACCTCTGGGAGTGGATCTCTCCAAATTCAAGCATTTGAGCATCATTTCCAAACGCTAAGGCAAGGAGGTGCTGTTTTGGTAGACCATCTGGAAATAGCAAACATTGATACTATCTTGAATTTTAATTTGAGTGGAGAAGCAATAGCAACAGTGGCTGAGTTCAAACTAGCCATAAATGCATATCTAGAAGACCTTGTGGTGTCCCCAGTTCGATCTTTGGCAGATATCATAGCCTTCAACTTGAAGTTTTCTGGTGTGGCAACAATGAGAAGGCCATTGTTAAATTTAGCAAAGCTGACAAAAGATGGATTTGAGAAACTAATGACATACAGCAGGCTAGATGCATTGGTGACTCCCGGTGCAGATATTGCTCCTGTACTTGCAATTGGGGGATTCCCAGGCATTAGTGTCCCTGCTGCATGTGACAAGAAGGGTGTGCCTTTCGGCATAACTTTTGGGGGTCTAAAGGGATCTGAACCAAAGCTTATACAGATTGCCTACGGTTTCGAGCAATCTACTAAGATTAGAAAGCCTCCCACATTTCTACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

474

Amino Acids

50.28

Weight (kDa)

9.41

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 59 - 271 1.2e-61 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
AccB7I CCANNNNNTGG 1 cut(s) 707
AccI GTMKAC 1 cut(s) 954
AciI CCGC 1 cut(s) 202
AclWI GGATC 4 cut(s) 589, 601, 895, 1351
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 4 cut(s) 616, 897, 991, 1156
AfaI GTAC 3 cut(s) 561, 640, 1251
AfeI AGCGCT 2 cut(s) 393, 448
AfiI CCNNNNNNNGG 5 cut(s) 441, 536, 707, 727, 774
AgsI TTSAA 8 cut(s) 137, 148, 194, 902, 991, 1036, 1111, 1117
AjnI CCWGG 3 cut(s) 298, 517, 1270
AluBI AGCT 8 cut(s) 119, 263, 367, 477, 626, 675, 1168, 1357
AluI AGCT 8 cut(s) 119, 263, 367, 477, 626, 675, 1168, 1357
Alw26I GTCTC 2 cut(s) 507, 638
AlwI GGATC 4 cut(s) 589, 601, 895, 1351
AlwNI CAGNNNCTG 2 cut(s) 791, 1028
Ama87I CYCGRG 1 cut(s) 414
Aor51HI AGCGCT 2 cut(s) 393, 448
AoxI GGCC 3 cut(s) 294, 538, 1145
ApeKI GCWGC 3 cut(s) 394, 611, 1289
ApoI RAATTY 4 cut(s) 616, 897, 991, 1156
Asp700I GAANNNNTTC 1 cut(s) 866
AspLEI GCGC 2 cut(s) 394, 449
AspS9I GGNCC 3 cut(s) 78, 295, 764
AsuC2I CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1230
AsuHPI GGTGA 6 cut(s) 250, 442, 504, 742, 865, 1234
AsuNHI GCTAGC 1 cut(s) 473
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 2 cut(s) 78, 764
BaeGI GKGCMC 1 cut(s) 414
BalI TGGCCA 1 cut(s) 540
BanII GRGCYC 2 cut(s) 493, 834
BbsI GAAGAC 1 cut(s) 1068
BbvI GCAGC 3 cut(s) 381, 623, 1276
BccI CCATC 5 cut(s) 25, 644, 776, 966, 1172
BciT130I CCWGG 3 cut(s) 300, 519, 1272
BcnI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1230
BcoDI GTCTC 2 cut(s) 507, 638
BfaI CTAG 5 cut(s) 59, 474, 1040, 1058, 1211
BfmI CTRYAG 1 cut(s) 772
BfoI RGCGCY 2 cut(s) 395, 450
BisI GCNGC 3 cut(s) 395, 612, 1290
BlsI GCNGC 3 cut(s) 396, 613, 1291
Bme1390I CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1230, 1272
Bme18I GGWCC 2 cut(s) 78, 764
BmeT110I CYCGRG 1 cut(s) 414
BmgT120I GGNCC 3 cut(s) 78, 295, 764
BmiI GGNNCC 6 cut(s) 79, 80, 297, 355, 454, 766
BmrFI CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1230, 1272
BmrI ACTGGG 1 cut(s) 1073
BmsI GCATC 7 cut(s) 238, 409, 702, 778, 796, 922, 1204
BmtI GCTAGC 1 cut(s) 477
BmuI ACTGGG 1 cut(s) 1073
BpiI GAAGAC 1 cut(s) 1068
BplI GAGNNNNNCTC 2 cut(s) 874, 906
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 3 cut(s) 291, 492, 929
BpuEI CTTGAG 3 cut(s) 323, 499, 818
BpuMI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1230
BsaI GGTCTC 2 cut(s) 507, 638
BsaJI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 817, 1270
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 5 cut(s) 441, 536, 707, 727, 774
Bse1I ACTGG 3 cut(s) 144, 737, 1079
BseBI CCWGG 3 cut(s) 300, 519, 1272
BseDI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 817, 1270
BseGI GGATG 2 cut(s) 253, 424
BseLI CCNNNNNNNGG 5 cut(s) 441, 536, 707, 727, 774
BseMII CTCAG 5 cut(s) 51, 506, 633, 696, 1020
BseNI ACTGG 3 cut(s) 144, 737, 1079
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 414
BseXI GCAGC 3 cut(s) 381, 623, 1276
BsgI GTGCAG 1 cut(s) 1254
BshFI GGCC 3 cut(s) 296, 540, 1147
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 6 cut(s) 215, 245, 312, 415, 726, 1230
BslFI GGGAC 3 cut(s) 64, 1060, 1268
BslI CCNNNNNNNGG 5 cut(s) 441, 536, 707, 727, 774
BsmAI GTCTC 2 cut(s) 507, 638
BsmFI GGGAC 3 cut(s) 64, 1060, 1268
BsmI GAATGC 1 cut(s) 324
BsnI GGCC 3 cut(s) 296, 540, 1147
Bso31I GGTCTC 2 cut(s) 507, 638
BsoBI CYCGRG 1 cut(s) 414
Bsp1286I GDGCHC 3 cut(s) 414, 493, 834
Bsp143I GATC 6 cut(s) 130, 581, 593, 887, 1085, 1343
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 1 cut(s) 202
BspANI GGCC 3 cut(s) 296, 540, 1147
BspCNI CTCAG 5 cut(s) 50, 505, 634, 695, 1021
BspLI GGNNCC 6 cut(s) 79, 80, 297, 355, 454, 766
BspMAI CTGCAG 1 cut(s) 776
BspOI GCTAGC 1 cut(s) 477
BspPI GGATC 4 cut(s) 589, 601, 895, 1351
BspTNI GGTCTC 2 cut(s) 507, 638
BsrI ACTGG 3 cut(s) 144, 737, 1079
BssECI CCNNGG 9 cut(s) 97, 298, 413, 414, 517, 541, 726, 817, 1270
BssMI GATC 6 cut(s) 130, 581, 593, 887, 1085, 1343
BssT1I CCWWGG 3 cut(s) 97, 541, 817
Bst2UI CCWGG 3 cut(s) 300, 519, 1272
Bst4CI ACNGT 5 cut(s) 94, 227, 706, 1024, 1376
Bst6I CTCTTC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstC8I GCNNGC 7 cut(s) 206, 317, 382, 424, 475, 673, 1208
BstDEI CTNAG 8 cut(s) 37, 291, 492, 642, 682, 929, 1029, 1392
BstDSI CCRYGG 1 cut(s) 97
BstEII GGTNACC 1 cut(s) 430
BstF5I GGATG 2 cut(s) 253, 424
BstH2I RGCGCY 2 cut(s) 395, 450
BstHHI GCGC 2 cut(s) 394, 449
BstKTI GATC 6 cut(s) 133, 584, 596, 890, 1088, 1346
BstMAI GTCTC 2 cut(s) 507, 638
BstMBI GATC 6 cut(s) 130, 581, 593, 887, 1085, 1343
BstMWI GCNNNNNNNGC 5 cut(s) 269, 321, 1025, 1049, 1241
BstNI CCWGG 3 cut(s) 300, 519, 1272
BstNSI RCATGY 1 cut(s) 1296
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 9 cut(s) 213, 243, 298, 413, 414, 517, 725, 1228, 1270
BstSFI CTRYAG 1 cut(s) 772
BstSLI GKGCMC 1 cut(s) 414
BstV1I GCAGC 3 cut(s) 381, 623, 1276
BstV2I GAAGAC 1 cut(s) 1068
BstX2I RGATCY 2 cut(s) 887, 1343
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 2 cut(s) 887, 1343
BsuRI GGCC 3 cut(s) 296, 540, 1147
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 2 cut(s) 253, 424
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 3 cut(s) 90, 595, 1029
Cac8I GCNNGC 7 cut(s) 206, 317, 382, 424, 475, 673, 1208
CaiI CAGNNNCTG 2 cut(s) 791, 1028
CfoI GCGC 2 cut(s) 394, 449
Cfr13I GGNCC 3 cut(s) 78, 295, 764
Cfr9I CCCGGG 1 cut(s) 414
Csp6I GTAC 3 cut(s) 560, 639, 1250
CviAII CATG 5 cut(s) 21, 98, 320, 576, 1293
CviQI GTAC 3 cut(s) 560, 639, 1250
DdeI CTNAG 8 cut(s) 37, 291, 492, 642, 682, 929, 1029, 1392
DpnI GATC 6 cut(s) 132, 583, 595, 889, 1087, 1345
DpnII GATC 6 cut(s) 130, 581, 593, 887, 1085, 1343
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 3 cut(s) 97, 541, 817
Eco24I GRGCYC 2 cut(s) 493, 834
Eco31I GGTCTC 2 cut(s) 507, 638
Eco32I GATATC 1 cut(s) 1099
Eco47I GGWCC 2 cut(s) 78, 764
Eco47III AGCGCT 2 cut(s) 393, 448
Eco88I CYCGRG 1 cut(s) 414
Eco91I GGTNACC 1 cut(s) 430
EcoO109I RGGNCCY 2 cut(s) 78, 295
EcoO65I GGTNACC 1 cut(s) 430
EcoRII CCWGG 3 cut(s) 298, 517, 1270
EcoRV GATATC 1 cut(s) 1099
EcoT14I CCWWGG 3 cut(s) 97, 541, 817
EcoT22I ATGCAT 2 cut(s) 1054, 1219
EcoT38I GRGCYC 2 cut(s) 493, 834
ErhI CCWWGG 3 cut(s) 97, 541, 817
FaeI CATG 5 cut(s) 24, 101, 323, 579, 1296
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 3 cut(s) 64, 1060, 1268
FatI CATG 5 cut(s) 20, 97, 319, 575, 1292
FblI GTMKAC 1 cut(s) 954
Fnu4HI GCNGC 3 cut(s) 395, 612, 1290
FokI GGATG 2 cut(s) 260, 431
FriOI GRGCYC 2 cut(s) 493, 834
Fsp4HI GCNGC 3 cut(s) 395, 612, 1290
FspBI CTAG 5 cut(s) 59, 474, 1040, 1058, 1211
GlaI GCGC 2 cut(s) 393, 448
GluI GCNGC 3 cut(s) 395, 612, 1290
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 2 cut(s) 395, 450
HaeIII GGCC 3 cut(s) 296, 540, 1147
HapII CCGG 6 cut(s) 215, 245, 312, 415, 726, 1230
HhaI GCGC 2 cut(s) 394, 449
Hin1II CATG 5 cut(s) 24, 101, 323, 579, 1296
Hin6I GCGC 2 cut(s) 392, 447
HinP1I GCGC 2 cut(s) 392, 447
HindIII AAGCTT 2 cut(s) 673, 1355
HinfI GANTC 6 cut(s) 52, 680, 711, 862, 1225, 1266
HpaII CCGG 6 cut(s) 215, 245, 312, 415, 726, 1230
HphI GGTGA 6 cut(s) 250, 442, 504, 742, 865, 1234
Hpy166II GTNNAC 2 cut(s) 241, 955
Hpy188I TCNGA 1 cut(s) 1348
Hpy188III TCNNGA 6 cut(s) 168, 194, 803, 962, 988, 1058
Hpy8I GTNNAC 2 cut(s) 241, 955
HpyAV CCTTC 6 cut(s) 200, 463, 877, 1117, 1137, 1297
HpyCH4III ACNGT 5 cut(s) 94, 227, 706, 1024, 1376
HpyCH4IV ACGT 1 cut(s) 509
HpyF10VI GCNNNNNNNGC 5 cut(s) 269, 321, 1025, 1049, 1241
HpyF3I CTNAG 8 cut(s) 37, 291, 492, 642, 682, 929, 1029, 1392
HpySE526I ACGT 1 cut(s) 509
Hsp92II CATG 5 cut(s) 24, 101, 323, 579, 1296
HspAI GCGC 2 cut(s) 392, 447
KflI GGGWCCC 1 cut(s) 78
Kzo9I GATC 6 cut(s) 130, 581, 593, 887, 1085, 1343
LmnI GCTCC 3 cut(s) 444, 452, 1249
Lsp1109I GCAGC 3 cut(s) 381, 623, 1276
LweI GCATC 7 cut(s) 238, 409, 702, 778, 796, 922, 1204
MaeI CTAG 5 cut(s) 59, 474, 1040, 1058, 1211
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 5 cut(s) 430, 510, 739, 1222, 1295
MalI GATC 6 cut(s) 132, 583, 595, 889, 1087, 1345
MboI GATC 6 cut(s) 130, 581, 593, 887, 1085, 1343
MboII GAAGA 5 cut(s) 25, 47, 125, 862, 1073
MfeI CAATTG 1 cut(s) 1257
MflI RGATCY 2 cut(s) 887, 1343
MhlI GDGCHC 3 cut(s) 414, 493, 834
MlsI TGGCCA 1 cut(s) 540
MluCI AATT 7 cut(s) 108, 616, 897, 991, 997, 1156, 1257
MluNI TGGCCA 1 cut(s) 540
MlyI GAGTC 3 cut(s) 674, 705, 1219
MmeI TCCRAC 1 cut(s) 688
MnlI CCTC 7 cut(s) 229, 435, 527, 852, 886, 932, 1416
Mox20I TGGCCA 1 cut(s) 540
Mph1103I ATGCAT 2 cut(s) 1054, 1219
MroXI GAANNNNTTC 1 cut(s) 866
MscI TGGCCA 1 cut(s) 540
MseI TTAA 4 cut(s) 65, 222, 996, 1154
MslI CAYNNNNRTG 2 cut(s) 19, 236
Msp20I TGGCCA 1 cut(s) 540
MspI CCGG 6 cut(s) 215, 245, 312, 415, 726, 1230
MspR9I CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1230, 1272
MunI CAATTG 1 cut(s) 1257
Mva1269I GAATGC 1 cut(s) 324
MvaI CCWGG 3 cut(s) 300, 519, 1272
MwoI GCNNNNNNNGC 5 cut(s) 269, 321, 1025, 1049, 1241
NciI CCSGG 6 cut(s) 215, 245, 415, 416, 727, 1230
NcoI CCATGG 1 cut(s) 97
NdeII GATC 6 cut(s) 130, 581, 593, 887, 1085, 1343
NheI GCTAGC 1 cut(s) 473
NlaIII CATG 5 cut(s) 24, 101, 323, 579, 1296
NlaIV GGNNCC 6 cut(s) 79, 80, 297, 355, 454, 766
NmeAIII GCCGAG 2 cut(s) 378, 746
NmuCI GTSAC 4 cut(s) 430, 510, 1222, 1295
NsiI ATGCAT 2 cut(s) 1054, 1219
NspI RCATGY 1 cut(s) 1296
PctI GAATGC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 866
PfeI GAWTC 3 cut(s) 52, 862, 1266
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 3 cut(s) 396, 613, 1291
PleI GAGTC 3 cut(s) 674, 705, 1219
PpsI GAGTC 3 cut(s) 674, 705, 1219
PpuMI RGGWCCY 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 78
Psp6I CCWGG 3 cut(s) 298, 517, 1270
PspEI GGTNACC 1 cut(s) 430
PspGI CCWGG 3 cut(s) 298, 517, 1270
PspN4I GGNNCC 6 cut(s) 79, 80, 297, 355, 454, 766
PspPI GGNCC 3 cut(s) 78, 295, 764
PspPPI RGGWCCY 1 cut(s) 78
PstI CTGCAG 1 cut(s) 776
PstNI CAGNNNCTG 2 cut(s) 791, 1028
PsuI RGATCY 2 cut(s) 887, 1343
RsaI GTAC 3 cut(s) 561, 640, 1251
RsaNI GTAC 3 cut(s) 560, 639, 1250
RseI CAYNNNNRTG 2 cut(s) 19, 236
SaqAI TTAA 4 cut(s) 65, 222, 996, 1154
SatI GCNGC 3 cut(s) 395, 612, 1290
Sau3AI GATC 6 cut(s) 130, 581, 593, 887, 1085, 1343
Sau96I GGNCC 3 cut(s) 78, 295, 764
SchI GAGTC 3 cut(s) 674, 705, 1219
ScrFI CCNGG 9 cut(s) 215, 245, 300, 415, 416, 519, 727, 1230, 1272
SduI GDGCHC 3 cut(s) 414, 493, 834
SfaNI GCATC 7 cut(s) 238, 409, 702, 778, 796, 922, 1204
SfcI CTRYAG 1 cut(s) 772
SinI GGWCC 2 cut(s) 78, 764
SmaI CCCGGG 1 cut(s) 416
SmiMI CAYNNNNRTG 2 cut(s) 19, 236
SmlI CTYRAG 3 cut(s) 338, 478, 833
SmoI CTYRAG 3 cut(s) 338, 478, 833
Sse9I AATT 7 cut(s) 108, 616, 897, 991, 997, 1156, 1257
SsiI CCGC 1 cut(s) 202
SspMI CTAG 5 cut(s) 59, 474, 1040, 1058, 1211
StyD4I CCNGG 9 cut(s) 213, 243, 298, 413, 414, 517, 725, 1228, 1270
StyI CCWWGG 3 cut(s) 97, 541, 817
TaaI ACNGT 5 cut(s) 94, 227, 706, 1024, 1376
TaiI ACGT 1 cut(s) 512
TaqI TCGA 2 cut(s) 1084, 1380
TaqII GACCGA 1 cut(s) 662
TasI AATT 7 cut(s) 108, 616, 897, 991, 997, 1156, 1257
TatI WGTACW 2 cut(s) 559, 1249
TfiI GAWTC 3 cut(s) 52, 862, 1266
Tru1I TTAA 4 cut(s) 65, 222, 996, 1154
Tru9I TTAA 4 cut(s) 65, 222, 996, 1154
TscAI CASTG 3 cut(s) 97, 595, 1029
TseFI GTSAC 4 cut(s) 430, 510, 1222, 1295
TseI GCWGC 3 cut(s) 394, 611, 1289
Tsp45I GTSAC 4 cut(s) 430, 510, 1222, 1295
TspMI CCCGGG 1 cut(s) 414
TspRI CASTG 3 cut(s) 97, 595, 1029
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 2 cut(s) 78, 764
XapI RAATTY 4 cut(s) 616, 897, 991, 1156
XbaI TCTAGA 1 cut(s) 1057
XceI RCATGY 1 cut(s) 1296
XmaI CCCGGG 1 cut(s) 414
XmiI GTMKAC 1 cut(s) 954
XmnI GAANNNNTTC 1 cut(s) 866
XspI CTAG 5 cut(s) 59, 474, 1040, 1058, 1211
Zsp2I ATGCAT 2 cut(s) 1054, 1219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.