pycom10g10370

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000427
Physical Location & Seq
Forward (+)
13632283 .. 13633858
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g10370.1

Sequence Viewer

Length: 930 bp
ATGAACGCAAGACTAAGGCGCCGTCCGCGTCACTCTCTAAGTTACATGGGATTCCAATTCTGGTCAAGGACAACATTGCTACCAAGGAAGTTGAACACCACAGCTGGTTCTTATGCTCTGCTTGGCTCGGTTGTTCCTCATGATGCGGGTGTTGTCACCAAGCTGAGGAATGCCGGGGCTATTAAACTGGGAAAGGCCAGCCTGAGTGAGTGGTCCTCTTGGAGGTCCAACGGTGCACCTTCCGGTTGGAGTGCCAGAGGTGGCCAAGGGGTGAACCCTTACAACTCTCTTGCAACACCTTGCGGCTCAAGTTGCGGTTCAGCAATATCAGCGGCAGCAAATCTGGCAACAGTGACACTCGGGACTGAAACAGATGGATCAATTTTATGTCCATCCAGTTTTAACTCAGACGTGGGCATCAAACCAACAAACGGTCTTACTAGTCGAGCAGGGGTAGTCCCAATCTCCCTAAGACAGGACACAGTTGGGCCAATTTGTAGGACAGTATCGGATGCTGCTCATGTTCTTGATGTCATTGCAGGCATCGACCGGAATGATGTTGCAACAACTGAGGCATCGAGGTACATTCCCATGGGTGGCTACGCACAGTTTCTCAGGCGCGACGGGCTTAGAGGAAAGCAAATAGGGATAGTGAGAGCCTTATTTAACATCACCGGCCAAACTTCTTTGACTCCAATTTATGAAAAACACTTCAACACGCTGAGGAAAGGTGGTGCTGTTTTGGTGGACAATTTGGAAATACCACATTTTGATGAAATCTATTCCGATAGAAGTAGTGAAACTACTGCACTGTCAGCTGAGTTCAAAATATATATAAACACATACCTTGAGGAGTTGGTGGCTTCGCCAGAGAAGGTGAAGGAGTATGGCCAGGCCTATTTTAAGAAGCTGAAGCAACAAATGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

310

Amino Acids

33.39

Weight (kDa)

9.49

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 30 - 268 3.1e-51 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 18
AccII CGCG 2 cut(s) 28, 621
AciI CCGC 5 cut(s) 26, 146, 303, 315, 332
AclWI GGATC 1 cut(s) 385
AcoI YGGCCR 3 cut(s) 262, 676, 889
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 1 cut(s) 584
AfiI CCNNNNNNNGG 5 cut(s) 165, 222, 431, 475, 596
AgsI TTSAA 3 cut(s) 94, 715, 826
AhlI ACTAGT 1 cut(s) 440
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 1 cut(s) 891
AluBI AGCT 4 cut(s) 104, 163, 818, 910
AluI AGCT 4 cut(s) 104, 163, 818, 910
Alw21I GWGCWC 1 cut(s) 238
Alw44I GTGCAC 1 cut(s) 234
AlwI GGATC 1 cut(s) 385
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 6 cut(s) 195, 262, 488, 676, 889, 894
ApaLI GTGCAC 1 cut(s) 234
ApeKI GCWGC 2 cut(s) 335, 515
AspLEI GCGC 2 cut(s) 21, 621
AspS9I GGNCC 3 cut(s) 213, 225, 488
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 4 cut(s) 148, 283, 664, 889
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 2 cut(s) 213, 225
BaeGI GKGCMC 1 cut(s) 238
BalI TGGCCA 2 cut(s) 264, 891
BanI GGYRCC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 238
BbvCI CCTCAGC 2 cut(s) 164, 722
BbvI GCAGC 2 cut(s) 347, 502
BccI CCATC 2 cut(s) 368, 400
BceAI ACGGC 1 cut(s) 6
BciT130I CCWGG 1 cut(s) 893
BcnI CCSGG 1 cut(s) 175
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 1 cut(s) 441
BfoI RGCGCY 1 cut(s) 22
BisI GCNGC 4 cut(s) 304, 333, 336, 516
BlsI GCNGC 4 cut(s) 305, 334, 337, 517
Bme1390I CCNGG 2 cut(s) 175, 893
Bme18I GGWCC 2 cut(s) 213, 225
BmeT110I CYCGRG 1 cut(s) 359
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 3 cut(s) 213, 225, 488
BmiI GGNNCC 1 cut(s) 20
BmrFI CCNGG 2 cut(s) 175, 893
BmrI ACTGGG 1 cut(s) 197
BmsI GCATC 5 cut(s) 133, 426, 502, 552, 584
BmuI ACTGGG 1 cut(s) 197
BplI GAGNNNNNCTC 2 cut(s) 200, 232
Bpu10I CCTNAGC 2 cut(s) 164, 722
BpuEI CTTGAG 2 cut(s) 292, 869
BpuMI CCSGG 1 cut(s) 175
BsaHI GRCGYC 1 cut(s) 19
BsaJI CCNNGG 4 cut(s) 83, 174, 265, 591
BsaWI WCCGGW 2 cut(s) 242, 549
Bsc4I CCNNNNNNNGG 5 cut(s) 165, 222, 431, 475, 596
Bse118I RCCGGY 1 cut(s) 674
Bse1I ACTGG 2 cut(s) 192, 396
Bse3DI GCAATG 2 cut(s) 74, 534
BseBI CCWGG 1 cut(s) 893
BseDI CCNNGG 4 cut(s) 83, 174, 265, 591
BseGI GGATG 2 cut(s) 392, 517
BseLI CCNNNNNNNGG 5 cut(s) 165, 222, 431, 475, 596
BseMI GCAATG 2 cut(s) 74, 534
BseMII CTCAG 7 cut(s) 155, 194, 420, 561, 628, 713, 810
BseNI ACTGG 2 cut(s) 192, 396
BseRI GAGGAG 1 cut(s) 866
BseSI GKGCMC 1 cut(s) 238
BseXI GCAGC 2 cut(s) 347, 502
BsgI GTGCAG 1 cut(s) 792
Bsh1236I CGCG 2 cut(s) 28, 621
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 6 cut(s) 197, 264, 490, 678, 891, 896
BshNI GGYRCC 1 cut(s) 18
BsiEI CGRYCG 1 cut(s) 550
BsiHKAI GWGCWC 1 cut(s) 238
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 4 cut(s) 174, 243, 550, 675
BslFI GGGAC 2 cut(s) 376, 443
BslI CCNNNNNNNGG 5 cut(s) 165, 222, 431, 475, 596
BsmFI GGGAC 2 cut(s) 376, 443
BsmI GAATGC 1 cut(s) 175
BsnI GGCC 6 cut(s) 197, 264, 490, 678, 891, 896
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 1 cut(s) 377
Bsp19I CCATGG 1 cut(s) 591
BspACI CCGC 5 cut(s) 26, 146, 303, 315, 332
BspANI GGCC 6 cut(s) 197, 264, 490, 678, 891, 896
BspCNI CTCAG 7 cut(s) 156, 195, 419, 562, 627, 714, 811
BspFNI CGCG 2 cut(s) 28, 621
BspHI TCATGA 1 cut(s) 139
BspLI GGNNCC 1 cut(s) 20
BspPI GGATC 1 cut(s) 385
BspT107I GGYRCC 1 cut(s) 18
BsrDI GCAATG 2 cut(s) 74, 534
BsrFI RCCGGY 1 cut(s) 674
BsrI ACTGG 2 cut(s) 192, 396
BssAI RCCGGY 1 cut(s) 674
BssECI CCNNGG 4 cut(s) 83, 174, 265, 591
BssMI GATC 1 cut(s) 377
BssNI GRCGYC 1 cut(s) 19
BssT1I CCWWGG 3 cut(s) 83, 265, 591
Bst2UI CCWGG 1 cut(s) 893
Bst4CI ACNGT 7 cut(s) 233, 352, 434, 484, 505, 609, 813
BstACI GRCGYC 1 cut(s) 19
BstC8I GCNNGC 2 cut(s) 199, 541
BstDSI CCRYGG 1 cut(s) 591
BstENI CCTNNNNNAGG 2 cut(s) 220, 473
BstF5I GGATG 2 cut(s) 392, 517
BstFNI CGCG 2 cut(s) 28, 621
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 2 cut(s) 21, 621
BstKTI GATC 1 cut(s) 380
BstMBI GATC 1 cut(s) 377
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 6 cut(s) 25, 312, 329, 344, 625, 815
BstNI CCWGG 1 cut(s) 893
BstSCI CCNGG 2 cut(s) 173, 891
BstSLI GKGCMC 1 cut(s) 238
BstUI CGCG 2 cut(s) 28, 621
BstV1I GCAGC 2 cut(s) 347, 502
BsuRI GGCC 6 cut(s) 197, 264, 490, 678, 891, 896
BtgI CCRYGG 1 cut(s) 591
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 2 cut(s) 392, 517
BtsIMutI CAGTG 2 cut(s) 357, 809
Cac8I GCNNGC 2 cut(s) 199, 541
CciI TCATGA 1 cut(s) 139
CfoI GCGC 2 cut(s) 21, 621
Cfr10I RCCGGY 1 cut(s) 674
Cfr13I GGNCC 3 cut(s) 213, 225, 488
CseI GACGC 1 cut(s) 17
Csp6I GTAC 1 cut(s) 583
CviAII CATG 4 cut(s) 46, 140, 521, 592
CviQI GTAC 1 cut(s) 583
DinI GGCGCC 1 cut(s) 20
DpnI GATC 1 cut(s) 379
DpnII GATC 1 cut(s) 377
EaeI YGGCCR 3 cut(s) 262, 676, 889
Eco130I CCWWGG 3 cut(s) 83, 265, 591
Eco147I AGGCCT 1 cut(s) 896
Eco47I GGWCC 2 cut(s) 213, 225
Eco88I CYCGRG 1 cut(s) 359
EcoNI CCTNNNNNAGG 2 cut(s) 220, 473
EcoRII CCWGG 1 cut(s) 891
EcoT14I CCWWGG 3 cut(s) 83, 265, 591
EgeI GGCGCC 1 cut(s) 20
EheI GGCGCC 1 cut(s) 20
ErhI CCWWGG 3 cut(s) 83, 265, 591
FaeI CATG 4 cut(s) 49, 143, 524, 595
FaqI GGGAC 2 cut(s) 376, 443
FatI CATG 4 cut(s) 45, 139, 520, 591
FauI CCCGC 1 cut(s) 139
Fnu4HI GCNGC 4 cut(s) 304, 333, 336, 516
FokI GGATG 2 cut(s) 379, 524
Fsp4HI GCNGC 4 cut(s) 304, 333, 336, 516
FspBI CTAG 1 cut(s) 441
GlaI GCGC 2 cut(s) 20, 620
GluI GCNGC 4 cut(s) 304, 333, 336, 516
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 6 cut(s) 197, 264, 490, 678, 891, 896
HapII CCGG 4 cut(s) 174, 243, 550, 675
HgaI GACGC 1 cut(s) 17
HhaI GCGC 2 cut(s) 21, 621
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 4 cut(s) 49, 143, 524, 595
Hin6I GCGC 2 cut(s) 19, 619
HinP1I GCGC 2 cut(s) 19, 619
HinfI GANTC 2 cut(s) 51, 691
HpaII CCGG 4 cut(s) 174, 243, 550, 675
HphI GGTGA 4 cut(s) 148, 283, 664, 889
Hpy166II GTNNAC 3 cut(s) 236, 274, 748
Hpy188I TCNGA 3 cut(s) 409, 511, 787
Hpy188III TCNNGA 3 cut(s) 140, 361, 527
Hpy8I GTNNAC 3 cut(s) 236, 274, 748
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 3 cut(s) 249, 868, 874
HpyCH4III ACNGT 7 cut(s) 233, 352, 434, 484, 505, 609, 813
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 5 cut(s) 236, 293, 539, 563, 809
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 312, 329, 344, 625, 815
HpySE526I ACGT 1 cut(s) 411
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 4 cut(s) 49, 143, 524, 595
HspAI GCGC 2 cut(s) 19, 619
KasI GGCGCC 1 cut(s) 18
Kzo9I GATC 1 cut(s) 377
Lsp1109I GCAGC 2 cut(s) 347, 502
LweI GCATC 5 cut(s) 133, 426, 502, 552, 584
MaeI CTAG 1 cut(s) 441
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 4 cut(s) 29, 41, 154, 352
MalI GATC 1 cut(s) 379
MboI GATC 1 cut(s) 377
MhlI GDGCHC 1 cut(s) 238
MlsI TGGCCA 2 cut(s) 264, 891
MluCI AATT 5 cut(s) 56, 381, 492, 696, 751
MluNI TGGCCA 2 cut(s) 264, 891
Mly113I GGCGCC 1 cut(s) 19
MlyI GAGTC 1 cut(s) 685
MmeI TCCRAC 2 cut(s) 227, 252
Mox20I TGGCCA 2 cut(s) 264, 891
MscI TGGCCA 2 cut(s) 264, 891
MseI TTAA 4 cut(s) 183, 402, 666, 903
MslI CAYNNNNRTG 2 cut(s) 590, 771
Msp20I TGGCCA 2 cut(s) 264, 891
MspA1I CMGCKG 3 cut(s) 104, 332, 818
MspI CCGG 4 cut(s) 174, 243, 550, 675
MspR9I CCNGG 2 cut(s) 175, 893
Mva1269I GAATGC 1 cut(s) 175
MvaI CCWGG 1 cut(s) 893
MvnI CGCG 2 cut(s) 28, 621
MwoI GCNNNNNNNGC 6 cut(s) 25, 312, 329, 344, 625, 815
NarI GGCGCC 1 cut(s) 19
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 591
NdeII GATC 1 cut(s) 377
NlaIII CATG 4 cut(s) 49, 143, 524, 595
NlaIV GGNNCC 1 cut(s) 20
NmuCI GTSAC 3 cut(s) 29, 154, 352
PagI TCATGA 1 cut(s) 139
PceI AGGCCT 1 cut(s) 896
PctI GAATGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 4 cut(s) 305, 334, 337, 517
PleI GAGTC 1 cut(s) 685
PluTI GGCGCC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 685
Psp6I CCWGG 1 cut(s) 891
PspGI CCWGG 1 cut(s) 891
PspN4I GGNNCC 1 cut(s) 20
PspPI GGNCC 3 cut(s) 213, 225, 488
PvuII CAGCTG 2 cut(s) 104, 818
RsaI GTAC 1 cut(s) 584
RsaNI GTAC 1 cut(s) 583
RseI CAYNNNNRTG 2 cut(s) 590, 771
SaqAI TTAA 4 cut(s) 183, 402, 666, 903
SatI GCNGC 4 cut(s) 304, 333, 336, 516
Sau3AI GATC 1 cut(s) 377
Sau96I GGNCC 3 cut(s) 213, 225, 488
SchI GAGTC 1 cut(s) 685
ScrFI CCNGG 2 cut(s) 175, 893
SduI GDGCHC 1 cut(s) 238
SfaNI GCATC 5 cut(s) 133, 426, 502, 552, 584
SfoI GGCGCC 1 cut(s) 20
SinI GGWCC 2 cut(s) 213, 225
SmiMI CAYNNNNRTG 2 cut(s) 590, 771
SmlI CTYRAG 2 cut(s) 307, 848
SmoI CTYRAG 2 cut(s) 307, 848
SpeI ACTAGT 1 cut(s) 440
Sse9I AATT 5 cut(s) 56, 381, 492, 696, 751
SseBI AGGCCT 1 cut(s) 896
SsiI CCGC 5 cut(s) 26, 146, 303, 315, 332
SspDI GGCGCC 1 cut(s) 18
SspMI CTAG 1 cut(s) 441
StuI AGGCCT 1 cut(s) 896
StyD4I CCNGG 2 cut(s) 173, 891
StyI CCWWGG 3 cut(s) 83, 265, 591
TaaI ACNGT 7 cut(s) 233, 352, 434, 484, 505, 609, 813
TaiI ACGT 1 cut(s) 414
TaqI TCGA 3 cut(s) 445, 546, 578
TasI AATT 5 cut(s) 56, 381, 492, 696, 751
TauI GCSGC 2 cut(s) 306, 335
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 4 cut(s) 183, 402, 666, 903
Tru9I TTAA 4 cut(s) 183, 402, 666, 903
TscAI CASTG 2 cut(s) 357, 816
TseFI GTSAC 3 cut(s) 29, 154, 352
TseI GCWGC 2 cut(s) 335, 515
Tsp45I GTSAC 3 cut(s) 29, 154, 352
TspDTI ATGAA 3 cut(s) 17, 717, 789
TspRI CASTG 2 cut(s) 357, 816
VneI GTGCAC 1 cut(s) 234
VpaK11BI GGWCC 2 cut(s) 213, 225
XagI CCTNNNNNAGG 2 cut(s) 220, 473
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.