Rh6DG020200

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000427
Physical Location & Seq
Reverse (-)
1785721 .. 1788132
2412 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG020200.1

Sequence Viewer

Length: 942 bp
ATGGTTTCTGTGACACTAGGGACGGAGACTGACGGCTCCATTATCTGTCCGGCTAGCTTTAACTCAGTGGTTGGCATCAAACCGACTGTTGGTCTCACTAGTCGAGCTGGAGTCATACCAATCAGTCCAAGACAGGACACAGTTGGGCCGATTTGTAGGACTGTAGCGGATGCTGTTCACGTCCTTGATATCATTGTTGGCATTGATAGGAATGACAATGCAACAAGTGAAGCATCAAAGTACATCCCAGTTGGTGGCTATGGACAGTTTCTCAAGCATAATGGCCTCGAAGGAAAGAGATTGGGGATAGTGAATATGTTATTTGATTCAATCAAGCACGGTGTTTTTCTGAAACGAACTTTTGATCAGCATTTTACCACACTGAGGAAACAGGGGGCAGTTTTGGTTGACAATCTTGAAATAGCCAATATTGAAGATGTTCTGGATTCTCATTCAAGTGGAGAATTTGATGCAATGTTAGCTGAATTCAAAATAGCCTTGAATGAGTACCTAAGTGACCTAGTAAAATCTCCAGTGAAGTCCTTAAGGGAGGTGATAGAGTTCAACAAGAATCATTCAAAGGTGGAAAAGATCAATGAATATGGGCAAGATGTCTTCGAATTAGCTGAGAAGACTAATGGGATGGGACCAAAAGAGCAGGAGGCATTGTCAAATTTAAAAAGACTATCTAAACAGGGGTTCAAGAAGTTGATGACCGATCATAGCCTAGATGCCTTGGTGGCTTATGCTAACAGTGCTTCTTCTATCTTCGCAATTGGTGGTTTTCCGGGCATTGTTGTGCCGGCAGGATATCATAACACTAAAAAGTATCCTTTTGGGATCTGCTTTGGGGGACTCAAGGGTTCAGAGCCAAAGCTGATTGAGATCGCCTATGCCTTTGAGCAAGCAACCAAGATAAGGAGAAAACCTCCTCTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

313

Amino Acids

34.05

Weight (kDa)

7.72

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 2 - 248 1.4e-27 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AciI CCGC 1 cut(s) 167
AclWI GGATC 1 cut(s) 848
AcsI RAATTY 3 cut(s) 464, 485, 673
AfaI GTAC 2 cut(s) 242, 509
AfiI CCNNNNNNNGG 4 cut(s) 89, 254, 384, 918
AflII CTTAAG 1 cut(s) 544
AgsI TTSAA 9 cut(s) 330, 419, 434, 456, 490, 502, 565, 579, 703
AhdI GACNNNNNGTC 1 cut(s) 90
AhlI ACTAGT 1 cut(s) 98
AjiI CACGTC 1 cut(s) 181
AluBI AGCT 5 cut(s) 57, 107, 482, 626, 877
AluI AGCT 5 cut(s) 57, 107, 482, 626, 877
Alw26I GTCTC 2 cut(s) 20, 98
AlwI GGATC 1 cut(s) 848
AoxI GGCC 2 cut(s) 146, 283
ApoI RAATTY 3 cut(s) 464, 485, 673
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 146, 647
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 1 cut(s) 565
AsuII TTCGAA 1 cut(s) 618
AsuNHI GCTAGC 1 cut(s) 53
AvaII GGWCC 1 cut(s) 647
BbsI GAAGAC 2 cut(s) 607, 638
BccI CCATC 1 cut(s) 637
BceAI ACGGC 1 cut(s) 49
BciVI GTATCC 1 cut(s) 840
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 2 cut(s) 20, 98
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 6 cut(s) 17, 54, 99, 521, 728, 935
BfmI CTRYAG 1 cut(s) 162
BfrI CTTAAG 1 cut(s) 544
BfuI GTATCC 1 cut(s) 840
BglI GCCNNNNNGGC 1 cut(s) 740
Bme1390I CCNGG 1 cut(s) 789
Bme18I GGWCC 1 cut(s) 647
BmeRI GACNNNNNGTC 1 cut(s) 90
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 2 cut(s) 146, 647
BmiI GGNNCC 2 cut(s) 37, 648
BmrFI CCNGG 1 cut(s) 789
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 5 cut(s) 84, 160, 242, 460, 721
BmtI GCTAGC 1 cut(s) 57
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 2 cut(s) 607, 638
BplI GAGNNNNNCTC 1 cut(s) 913
BpmI CTGGAG 2 cut(s) 129, 516
Bpu14I TTCGAA 1 cut(s) 618
BpuEI CTTGAG 2 cut(s) 257, 842
BpuMI CCSGG 1 cut(s) 789
BsaBI GATNNNNATC 1 cut(s) 884
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 735
BsaXI ACNNNNNCTCC 2 cut(s) 653, 683
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 254, 384, 918
Bse118I RCCGGY 1 cut(s) 802
Bse1I ACTGG 2 cut(s) 248, 533
Bse3DI GCAATG 1 cut(s) 480
Bse8I GATNNNNATC 1 cut(s) 884
BseDI CCNNGG 1 cut(s) 735
BseGI GGATG 3 cut(s) 175, 243, 648
BseJI GATNNNNATC 1 cut(s) 884
BseLI CCNNNNNNNGG 4 cut(s) 89, 254, 384, 918
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 3 cut(s) 78, 374, 618
BseNI ACTGG 2 cut(s) 248, 533
BseRI GAGGAG 1 cut(s) 921
BshFI GGCC 2 cut(s) 148, 285
BsiSI CCGG 3 cut(s) 50, 788, 803
BslFI GGGAC 3 cut(s) 34, 660, 867
BslI CCNNNNNNNGG 4 cut(s) 89, 254, 384, 918
BsmAI GTCTC 2 cut(s) 20, 98
BsmFI GGGAC 3 cut(s) 34, 660, 867
BsnI GGCC 2 cut(s) 148, 285
Bso31I GGTCTC 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 618
Bsp143I GATC 5 cut(s) 364, 591, 718, 840, 885
BspACI CCGC 1 cut(s) 167
BspANI GGCC 2 cut(s) 148, 285
BspCNI CTCAG 3 cut(s) 77, 375, 619
BspLI GGNNCC 2 cut(s) 37, 648
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 1 cut(s) 848
BspT104I TTCGAA 1 cut(s) 618
BspTI CTTAAG 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 802
BsrI ACTGG 2 cut(s) 248, 533
BssAI RCCGGY 1 cut(s) 802
BssECI CCNNGG 1 cut(s) 735
BssMI GATC 5 cut(s) 364, 591, 718, 840, 885
BssT1I CCWWGG 1 cut(s) 735
Bst4CI ACNGT 6 cut(s) 88, 142, 163, 267, 341, 755
BstAFI CTTAAG 1 cut(s) 544
BstBI TTCGAA 1 cut(s) 618
BstC8I GCNNGC 3 cut(s) 55, 804, 906
BstDEI CTNAG 4 cut(s) 64, 383, 512, 627
BstF5I GGATG 3 cut(s) 175, 243, 648
BstKTI GATC 5 cut(s) 367, 594, 721, 843, 888
BstMAI GTCTC 2 cut(s) 20, 98
BstMBI GATC 5 cut(s) 364, 591, 718, 840, 885
BstMWI GCNNNNNNNGC 3 cut(s) 479, 740, 755
BstSCI CCNGG 1 cut(s) 787
BstSFI CTRYAG 1 cut(s) 162
BstV2I GAAGAC 2 cut(s) 607, 638
BstX2I RGATCY 1 cut(s) 840
BstYI RGATCY 1 cut(s) 840
BsuI GTATCC 1 cut(s) 840
BsuRI GGCC 2 cut(s) 148, 285
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 3 cut(s) 175, 243, 648
BtsIMutI CAGTG 4 cut(s) 72, 380, 540, 760
Cac8I GCNNGC 3 cut(s) 55, 804, 906
Cfr10I RCCGGY 1 cut(s) 802
Cfr13I GGNCC 2 cut(s) 146, 647
Csp6I GTAC 2 cut(s) 241, 508
CviQI GTAC 2 cut(s) 241, 508
DdeI CTNAG 4 cut(s) 64, 383, 512, 627
DpnI GATC 5 cut(s) 366, 593, 720, 842, 887
DpnII GATC 5 cut(s) 364, 591, 718, 840, 885
DraI TTTAAA 1 cut(s) 678
DriI GACNNNNNGTC 1 cut(s) 90
Eam1105I GACNNNNNGTC 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 735
Eco31I GGTCTC 1 cut(s) 98
Eco32I GATATC 2 cut(s) 190, 812
Eco47I GGWCC 1 cut(s) 647
EcoRI GAATTC 1 cut(s) 485
EcoRV GATATC 2 cut(s) 190, 812
EcoT14I CCWWGG 1 cut(s) 735
ErhI CCWWGG 1 cut(s) 735
FaiI YATR 9 cut(s) 116, 261, 279, 317, 603, 723, 747, 816, 894
FaqI GGGAC 3 cut(s) 34, 660, 867
FbaI TGATCA 1 cut(s) 364
FokI GGATG 3 cut(s) 182, 230, 655
FspBI CTAG 6 cut(s) 17, 54, 99, 521, 728, 935
GsuI CTGGAG 2 cut(s) 129, 516
HaeIII GGCC 2 cut(s) 148, 285
HapII CCGG 3 cut(s) 50, 788, 803
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 5 cut(s) 111, 326, 446, 571, 855
HpaII CCGG 3 cut(s) 50, 788, 803
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 2 cut(s) 178, 409
Hpy188I TCNGA 2 cut(s) 351, 868
Hpy188III TCNNGA 3 cut(s) 416, 443, 703
Hpy8I GTNNAC 2 cut(s) 178, 409
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 6 cut(s) 88, 142, 163, 267, 341, 755
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 2 cut(s) 221, 473
HpyF10VI GCNNNNNNNGC 3 cut(s) 479, 740, 755
HpyF3I CTNAG 4 cut(s) 64, 383, 512, 627
HpySE526I ACGT 1 cut(s) 180
KroI GCCGGC 1 cut(s) 802
KroNI GCCGGC 1 cut(s) 804
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 5 cut(s) 364, 591, 718, 840, 885
LmnI GCTCC 1 cut(s) 41
LweI GCATC 5 cut(s) 84, 160, 242, 460, 721
MaeI CTAG 6 cut(s) 17, 54, 99, 521, 728, 935
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 10, 515
MalI GATC 5 cut(s) 366, 593, 720, 842, 887
MboI GATC 5 cut(s) 364, 591, 718, 840, 885
MboII GAAGA 5 cut(s) 446, 607, 643, 753, 760
MfeI CAATTG 1 cut(s) 774
MflI RGATCY 1 cut(s) 840
MluCI AATT 5 cut(s) 464, 485, 620, 673, 774
MlyI GAGTC 2 cut(s) 120, 849
MnlI CCTC 6 cut(s) 296, 378, 544, 655, 939, 942
MroNI GCCGGC 1 cut(s) 802
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 3 cut(s) 60, 545, 677
MslI CAYNNNNRTG 2 cut(s) 456, 797
MspCI CTTAAG 1 cut(s) 544
MspI CCGG 3 cut(s) 50, 788, 803
MspR9I CCNGG 1 cut(s) 789
MunI CAATTG 1 cut(s) 774
MwoI GCNNNNNNNGC 3 cut(s) 479, 740, 755
NaeI GCCGGC 1 cut(s) 804
NciI CCSGG 1 cut(s) 789
NdeII GATC 5 cut(s) 364, 591, 718, 840, 885
NgoMIV GCCGGC 1 cut(s) 802
NheI GCTAGC 1 cut(s) 53
NlaIV GGNNCC 2 cut(s) 37, 648
NmuCI GTSAC 2 cut(s) 10, 515
NspV TTCGAA 1 cut(s) 618
PdiI GCCGGC 1 cut(s) 804
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 3 cut(s) 326, 446, 571
PflMI CCANNNNNTGG 1 cut(s) 254
PleI GAGTC 2 cut(s) 119, 849
PpsI GAGTC 2 cut(s) 119, 849
PspN4I GGNNCC 2 cut(s) 37, 648
PspPI GGNCC 2 cut(s) 146, 647
PsuI RGATCY 1 cut(s) 840
RsaI GTAC 2 cut(s) 242, 509
RsaNI GTAC 2 cut(s) 241, 508
RseI CAYNNNNRTG 2 cut(s) 456, 797
SaqAI TTAA 3 cut(s) 60, 545, 677
Sau3AI GATC 5 cut(s) 364, 591, 718, 840, 885
Sau96I GGNCC 2 cut(s) 146, 647
SchI GAGTC 2 cut(s) 120, 849
ScrFI CCNGG 1 cut(s) 789
SfaNI GCATC 5 cut(s) 84, 160, 242, 460, 721
SfcI CTRYAG 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 618
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 2 cut(s) 456, 797
SmlI CTYRAG 3 cut(s) 272, 544, 857
SmoI CTYRAG 3 cut(s) 272, 544, 857
SpeI ACTAGT 1 cut(s) 98
Sse9I AATT 5 cut(s) 464, 485, 620, 673, 774
SsiI CCGC 1 cut(s) 167
SspI AATATT 1 cut(s) 430
SspMI CTAG 6 cut(s) 17, 54, 99, 521, 728, 935
StyD4I CCNGG 1 cut(s) 787
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 6 cut(s) 88, 142, 163, 267, 341, 755
TaiI ACGT 1 cut(s) 183
TaqI TCGA 3 cut(s) 103, 288, 618
TaqII GACCGA 1 cut(s) 731
TasI AATT 5 cut(s) 464, 485, 620, 673, 774
TatI WGTACW 1 cut(s) 240
TfiI GAWTC 3 cut(s) 326, 446, 571
Tru1I TTAA 3 cut(s) 60, 545, 677
Tru9I TTAA 3 cut(s) 60, 545, 677
TscAI CASTG 4 cut(s) 72, 387, 540, 760
TseFI GTSAC 2 cut(s) 10, 515
Tsp45I GTSAC 2 cut(s) 10, 515
TspDTI ATGAA 1 cut(s) 612
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 4 cut(s) 72, 387, 540, 760
Van91I CCANNNNNTGG 1 cut(s) 254
Vha464I CTTAAG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 3 cut(s) 464, 485, 673
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 6 cut(s) 17, 54, 99, 521, 728, 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.