Prupe.8G161800_v2.0.a1

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000427
Physical Location & Seq
Forward (+)
16981871 .. 16984265
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G161800.1

Sequence Viewer

Length: 1593 bp
ATGGCAACTGGTGGTGGTGGTGGCTCTCCGTTGATGAATAGAGGGCAAGGGTTCTCAATCAGGGAAGCCACCATAGATGATCTCCAACTTGCTTTCAAGCACAACCAGCTCACCTCAAGGCAACTTGTCCAATTTTACCTTGGAGAAATCACCAGACTCAACCCTTTCCTCAAGGGGGTCATAGAAGTGAACCTGGATGCTCTTGACCTTGCCGAAAAGGCTGACTACGAGCGCAAGACCAAGCCACCATCTGTGCCACTCTCTAAGTTGCATGGCATTCCCATTCTGATCAAGGACAACACTGCAACCAAGGACAAGTTGAACACCACAGCTGGCTCTTATGCTCTGCTTGGCTCTGTTGTGCCCCGGGACGCCGGCGTGGTGACCAAGTTGAGGGAGGCTGGGGCCATCATACTGGGAAAGGCCAGCTTGAGTGAGTGGTCCAATTGGAGGTCCAATAGTGCACCTTCCGGTTGGAATGCCAGAGGTGGCCAAGGAGTGAACCCGTACAATTTTGAGGAGACGCCTAGCGGCTCAAGCAGTGGGTCAGCAATATCAGTGGCAGCAAGTCTGGTAGCAGTGGCACTGGGGACTGAGACAGATGGATCAATTTTAAGTCCATGCAGTTATAACTCAGTAGTTGGCATCAAACCAACAGTTGGTCTTACTAGTCGAGCAGGAGTTGTCCCGATCTCCCCGAGACAGGACACAGTTGGGTATGTAGCATTAATTTTGGATACCTTTAATAGTCAGATGACTAGTTTTTTCTCATTGTTTTTTGGTGTTGGATTGAGCAGGCCCATCTGTAGGACAGTATCAGATGCTGCTTATGTTCTTGACGTCATTGCAGGCGCTGACAACATTGATATTGCAACAATTGAGACATCGAAGTACATTCCCGAGGGTGGCTATGCACAATTTCTCAAATGCGATGGACTTGGAGGAAAGAGATTAGGGATATTGAGAGCCTTCTATGACTTGGAGGATGACCCTTTCTTGACTCAAATTTTTGAAAAACATCTCAACACACTGAGGAATGGAGGTGCTATTTTGGTAGACCATTTGGAAATAGCCAATTTGGACAAAATCGTGTTCGATATTGGAAGTGATGAACAGACTGCATTGTCTGCTGAGTTCAAAATATCCTTAAACACATACCTTAAGGATTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTGATAGCCTACAACAATAAAAACTCGAAATTGGAAAAGATCAATGAATATGGACAAGATCTCTTAGAAGAGTCTGAGAAGACTAATGGAATAGGACCAAAAGAGCTGGAAGTGTTATCAAATTTAGAAAGACTGTCTAGAGATGGATTTGAGAAGCTAATGACAGATAAGAGCCTAGATGCCTTGGTGACTTATTCTAGCACTGCTTCTTCTATCCTTGCAATTGGTGGGTTTCCGGGCATTATTGTGCCGGCAGGATTCCATGACACTGAAAAGTACCCTTTTGGAATCTGCTTTGGGGGGCTAAAGGGTTCAGAGCCAAAGCTGATTGAGATTGCATACGCGTTTGAGCAAGCAACTAAGATCAGGAAGCCTCCACCTGTTGGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

531

Amino Acids

56.77

Weight (kDa)

5.34

Isoelectric Point (pI)

34.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
AasI GACNNNNNNGTC 1 cut(s) 1123
AatII GACGTC 1 cut(s) 843
AccB7I CCANNNNNTGG 2 cut(s) 659, 1583
AccI GTMKAC 1 cut(s) 1056
AccII CGCG 1 cut(s) 1544
AciI CCGC 1 cut(s) 531
AclWI GGATC 2 cut(s) 613, 1182
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 2 cut(s) 1005, 1321
AcyI GRCGYC 3 cut(s) 372, 524, 840
AfaI GTAC 3 cut(s) 509, 893, 1478
AfiI CCNNNNNNNGG 8 cut(s) 175, 393, 450, 659, 703, 807, 905, 1583
AflII CTTAAG 1 cut(s) 1160
AflIII ACRYGT 1 cut(s) 1542
AgsI TTSAA 4 cut(s) 97, 322, 1013, 1138
AhlI ACTAGT 2 cut(s) 668, 758
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 6 cut(s) 109, 332, 429, 1306, 1357, 1525
AluI AGCT 6 cut(s) 109, 332, 429, 1306, 1357, 1525
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 4 cut(s) 515, 590, 694, 875
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 2 cut(s) 613, 1182
AlwNI CAGNNNCTG 2 cut(s) 824, 854
Ama87I CYCGRG 3 cut(s) 366, 697, 899
AoxI GGCC 4 cut(s) 405, 423, 490, 797
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 2 cut(s) 563, 824
ApoI RAATTY 2 cut(s) 1005, 1321
AseI ATTAAT 1 cut(s) 728
AspLEI GCGC 2 cut(s) 234, 854
AspS9I GGNCC 5 cut(s) 405, 441, 453, 798, 1295
AsuC2I CCSGG 3 cut(s) 367, 368, 1437
AsuHPI GGTGA 4 cut(s) 103, 142, 394, 1399
AvaI CYCGRG 3 cut(s) 366, 697, 899
AvaII GGWCC 3 cut(s) 441, 453, 1295
BaeGI GKGCMC 2 cut(s) 366, 466
BalI TGGCCA 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 1286
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 2 cut(s) 575, 811
BccI CCATC 6 cut(s) 256, 416, 596, 809, 926, 1337
BciT130I CCWGG 1 cut(s) 194
BciVI GTATCC 1 cut(s) 730
BclI TGATCA 1 cut(s) 288
BcnI CCSGG 3 cut(s) 367, 368, 1437
BcoDI GTCTC 4 cut(s) 515, 590, 694, 875
BcuI ACTAGT 2 cut(s) 668, 758
BfaI CTAG 6 cut(s) 528, 669, 759, 1338, 1376, 1398
BfmI CTRYAG 1 cut(s) 805
BfoI RGCGCY 1 cut(s) 855
BfrI CTTAAG 1 cut(s) 1160
BfuI GTATCC 1 cut(s) 730
BglI GCCNNNNNGGC 1 cut(s) 218
BglII AGATCT 1 cut(s) 1258
BisI GCNGC 3 cut(s) 532, 564, 825
BlsI GCNGC 3 cut(s) 533, 565, 826
Bme1390I CCNGG 4 cut(s) 194, 367, 368, 1437
Bme18I GGWCC 3 cut(s) 441, 453, 1295
BmeT110I CYCGRG 3 cut(s) 366, 697, 899
BmgT120I GGNCC 5 cut(s) 405, 441, 453, 798, 1295
BmiI GGNNCC 1 cut(s) 406
BmrFI CCNGG 4 cut(s) 194, 367, 368, 1437
BmrI ACTGGG 3 cut(s) 425, 596, 1175
BmsI GCATC 4 cut(s) 187, 654, 811, 1369
BmuI ACTGGG 3 cut(s) 425, 596, 1175
BpiI GAAGAC 1 cut(s) 1286
BpuEI CTTGAG 4 cut(s) 100, 155, 451, 520
BpuMI CCSGG 3 cut(s) 367, 368, 1437
BsaHI GRCGYC 3 cut(s) 372, 524, 840
BsaJI CCNNGG 8 cut(s) 139, 309, 365, 366, 493, 900, 1191, 1383
BsaWI WCCGGW 1 cut(s) 470
Bsc4I CCNNNNNNNGG 8 cut(s) 175, 393, 450, 659, 703, 807, 905, 1583
Bse118I RCCGGY 2 cut(s) 374, 1450
Bse1I ACTGG 4 cut(s) 13, 420, 591, 1181
Bse3DI GCAATG 1 cut(s) 843
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 8 cut(s) 139, 309, 365, 366, 493, 900, 1191, 1383
BseGI GGATG 2 cut(s) 202, 991
BseLI CCNNNNNNNGG 8 cut(s) 175, 393, 450, 659, 703, 807, 905, 1583
BseMI GCAATG 1 cut(s) 843
BseMII CTCAG 5 cut(s) 585, 648, 1022, 1122, 1266
BseNI ACTGG 4 cut(s) 13, 420, 591, 1181
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 2 cut(s) 366, 466
BseXI GCAGC 2 cut(s) 575, 811
BseYI CCCAGC 1 cut(s) 401
Bsh1236I CGCG 1 cut(s) 1544
BshFI GGCC 4 cut(s) 407, 425, 492, 799
BsiHKAI GWGCWC 1 cut(s) 466
BsiHKCI CYCGRG 3 cut(s) 366, 697, 899
BsiSI CCGG 5 cut(s) 367, 375, 471, 1436, 1451
BslFI GGGAC 3 cut(s) 383, 604, 671
BslI CCNNNNNNNGG 8 cut(s) 175, 393, 450, 659, 703, 807, 905, 1583
BsmAI GTCTC 4 cut(s) 515, 590, 694, 875
BsmBI CGTCTC 1 cut(s) 515
BsmFI GGGAC 3 cut(s) 383, 604, 671
BsmI GAATGC 2 cut(s) 276, 484
BsnI GGCC 4 cut(s) 407, 425, 492, 799
BsoBI CYCGRG 3 cut(s) 366, 697, 899
Bsp1286I GDGCHC 2 cut(s) 366, 466
Bsp143I GATC 8 cut(s) 79, 288, 605, 690, 1187, 1239, 1258, 1563
BspACI CCGC 1 cut(s) 531
BspANI GGCC 4 cut(s) 407, 425, 492, 799
BspCNI CTCAG 5 cut(s) 586, 647, 1023, 1123, 1267
BspFNI CGCG 1 cut(s) 1544
BspLI GGNNCC 1 cut(s) 406
BspPI GGATC 2 cut(s) 613, 1182
BspTI CTTAAG 1 cut(s) 1160
BsrDI GCAATG 1 cut(s) 843
BsrFI RCCGGY 2 cut(s) 374, 1450
BsrI ACTGG 4 cut(s) 13, 420, 591, 1181
BssAI RCCGGY 2 cut(s) 374, 1450
BssECI CCNNGG 8 cut(s) 139, 309, 365, 366, 493, 900, 1191, 1383
BssMI GATC 8 cut(s) 79, 288, 605, 690, 1187, 1239, 1258, 1563
BssNI GRCGYC 3 cut(s) 372, 524, 840
BssT1I CCWWGG 5 cut(s) 139, 309, 493, 1191, 1383
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 4 cut(s) 658, 712, 814, 1335
Bst6I CTCTTC 1 cut(s) 1263
BstACI GRCGYC 3 cut(s) 372, 524, 840
BstAFI CTTAAG 1 cut(s) 1160
BstAPI GCANNNNNTGC 1 cut(s) 1127
BstC8I GCNNGC 7 cut(s) 334, 376, 427, 797, 850, 1452, 1554
BstDEI CTNAG 8 cut(s) 264, 594, 634, 1031, 1131, 1264, 1275, 1560
BstEII GGTNACC 1 cut(s) 382
BstF5I GGATG 2 cut(s) 202, 991
BstFNI CGCG 1 cut(s) 1544
BstH2I RGCGCY 1 cut(s) 855
BstHHI GCGC 2 cut(s) 234, 854
BstKTI GATC 8 cut(s) 82, 291, 608, 693, 1190, 1242, 1261, 1566
BstMAI GTCTC 4 cut(s) 515, 590, 694, 875
BstMBI GATC 8 cut(s) 79, 288, 605, 690, 1187, 1239, 1258, 1563
BstMWI GCNNNNNNNGC 4 cut(s) 106, 218, 537, 1127
BstNI CCWGG 1 cut(s) 194
BstPI GGTNACC 1 cut(s) 382
BstSCI CCNGG 4 cut(s) 192, 365, 366, 1435
BstSFI CTRYAG 1 cut(s) 805
BstSLI GKGCMC 2 cut(s) 366, 466
BstUI CGCG 1 cut(s) 1544
BstV1I GCAGC 2 cut(s) 575, 811
BstV2I GAAGAC 1 cut(s) 1286
BstX2I RGATCY 1 cut(s) 1258
BstXI CCANNNNNNTGG 1 cut(s) 415
BstYI RGATCY 1 cut(s) 1258
BsuI GTATCC 1 cut(s) 730
BsuRI GGCC 4 cut(s) 407, 425, 492, 799
BtgZI GCGATG 1 cut(s) 945
BtsCI GGATG 2 cut(s) 202, 991
BtsI GCAGTG 4 cut(s) 300, 547, 585, 1401
BtsIMutI CAGTG 9 cut(s) 300, 547, 564, 584, 585, 1028, 1188, 1401, 1467
Cac8I GCNNGC 7 cut(s) 334, 376, 427, 797, 850, 1452, 1554
CaiI CAGNNNCTG 2 cut(s) 824, 854
CfoI GCGC 2 cut(s) 234, 854
Cfr10I RCCGGY 2 cut(s) 374, 1450
Cfr13I GGNCC 5 cut(s) 405, 441, 453, 798, 1295
Cfr9I CCCGGG 1 cut(s) 366
CseI GACGC 2 cut(s) 380, 532
Csp6I GTAC 3 cut(s) 508, 892, 1477
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviAII CATG 3 cut(s) 272, 621, 1463
CviQI GTAC 3 cut(s) 508, 892, 1477
DdeI CTNAG 8 cut(s) 264, 594, 634, 1031, 1131, 1264, 1275, 1560
DpnI GATC 8 cut(s) 81, 290, 607, 692, 1189, 1241, 1260, 1565
DpnII GATC 8 cut(s) 79, 288, 605, 690, 1187, 1239, 1258, 1563
DrdI GACNNNNNNGTC 1 cut(s) 1123
DseDI GACNNNNNNGTC 1 cut(s) 1123
EaeI YGGCCR 1 cut(s) 490
Eam1104I CTCTTC 1 cut(s) 1263
EarI CTCTTC 1 cut(s) 1263
Eco130I CCWWGG 5 cut(s) 139, 309, 493, 1191, 1383
Eco47I GGWCC 3 cut(s) 441, 453, 1295
Eco88I CYCGRG 3 cut(s) 366, 697, 899
Eco91I GGTNACC 1 cut(s) 382
EcoO65I GGTNACC 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 192
EcoT14I CCWWGG 5 cut(s) 139, 309, 493, 1191, 1383
ErhI CCWWGG 5 cut(s) 139, 309, 493, 1191, 1383
Esp3I CGTCTC 1 cut(s) 515
FaeI CATG 3 cut(s) 275, 624, 1466
FalI AAGNNNNNCTT 2 cut(s) 413, 445
FaqI GGGAC 3 cut(s) 383, 604, 671
FatI CATG 3 cut(s) 271, 620, 1462
FbaI TGATCA 1 cut(s) 288
FblI GTMKAC 1 cut(s) 1056
Fnu4HI GCNGC 3 cut(s) 532, 564, 825
FokI GGATG 2 cut(s) 209, 998
Fsp4HI GCNGC 3 cut(s) 532, 564, 825
FspBI CTAG 6 cut(s) 528, 669, 759, 1338, 1376, 1398
GlaI GCGC 2 cut(s) 233, 853
GluI GCNGC 3 cut(s) 532, 564, 825
GsaI CCCAGC 1 cut(s) 405
HaeII RGCGCY 1 cut(s) 855
HaeIII GGCC 4 cut(s) 407, 425, 492, 799
HapII CCGG 5 cut(s) 367, 375, 471, 1436, 1451
HgaI GACGC 2 cut(s) 380, 532
HhaI GCGC 2 cut(s) 234, 854
Hin1I GRCGYC 3 cut(s) 372, 524, 840
Hin1II CATG 3 cut(s) 275, 624, 1466
Hin6I GCGC 2 cut(s) 232, 852
HinP1I GCGC 2 cut(s) 232, 852
HinfI GANTC 5 cut(s) 156, 1000, 1271, 1458, 1488
HpaII CCGG 5 cut(s) 367, 375, 471, 1436, 1451
HphI GGTGA 4 cut(s) 103, 142, 394, 1399
Hpy166II GTNNAC 4 cut(s) 190, 464, 502, 1057
Hpy188I TCNGA 5 cut(s) 288, 753, 820, 1276, 1516
Hpy188III TCNNGA 7 cut(s) 203, 688, 836, 899, 997, 1338, 1567
Hpy8I GTNNAC 4 cut(s) 190, 464, 502, 1057
HpyAV CCTTC 2 cut(s) 477, 979
HpyCH4III ACNGT 4 cut(s) 658, 712, 814, 1335
HpyCH4IV ACGT 1 cut(s) 840
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 218, 537, 1127
HpyF3I CTNAG 8 cut(s) 264, 594, 634, 1031, 1131, 1264, 1275, 1560
HpySE526I ACGT 1 cut(s) 840
Hsp92I GRCGYC 3 cut(s) 372, 524, 840
Hsp92II CATG 3 cut(s) 275, 624, 1466
HspAI GCGC 2 cut(s) 232, 852
KroI GCCGGC 2 cut(s) 374, 1450
KroNI GCCGGC 2 cut(s) 376, 1452
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 8 cut(s) 79, 288, 605, 690, 1187, 1239, 1258, 1563
Lsp1109I GCAGC 2 cut(s) 575, 811
LweI GCATC 4 cut(s) 187, 654, 811, 1369
MaeI CTAG 6 cut(s) 528, 669, 759, 1338, 1376, 1398
MaeII ACGT 1 cut(s) 840
MaeIII GTNAC 2 cut(s) 382, 1387
MalI GATC 8 cut(s) 81, 290, 607, 692, 1189, 1241, 1260, 1565
MboI GATC 8 cut(s) 79, 288, 605, 690, 1187, 1239, 1258, 1563
MboII GAAGA 3 cut(s) 1280, 1291, 1401
MfeI CAATTG 3 cut(s) 445, 876, 1422
MflI RGATCY 1 cut(s) 1258
MhlI GDGCHC 2 cut(s) 366, 466
MlsI TGGCCA 1 cut(s) 492
MluI ACGCGT 1 cut(s) 1542
MluNI TGGCCA 1 cut(s) 492
MlyI GAGTC 3 cut(s) 150, 994, 1280
MmeI TCCRAC 3 cut(s) 109, 455, 766
Mox20I TGGCCA 1 cut(s) 492
MreI CGCCGGCG 1 cut(s) 374
MroNI GCCGGC 2 cut(s) 374, 1450
MscI TGGCCA 1 cut(s) 492
MseI TTAA 6 cut(s) 614, 728, 744, 1148, 1161, 1591
MslI CAYNNNNRTG 2 cut(s) 185, 1445
Msp20I TGGCCA 1 cut(s) 492
MspA1I CMGCKG 1 cut(s) 332
MspCI CTTAAG 1 cut(s) 1160
MspI CCGG 5 cut(s) 367, 375, 471, 1436, 1451
MspR9I CCNGG 4 cut(s) 194, 367, 368, 1437
MunI CAATTG 3 cut(s) 445, 876, 1422
Mva1269I GAATGC 2 cut(s) 276, 484
MvaI CCWGG 1 cut(s) 194
MvnI CGCG 1 cut(s) 1544
MwoI GCNNNNNNNGC 4 cut(s) 106, 218, 537, 1127
NaeI GCCGGC 2 cut(s) 376, 1452
NciI CCSGG 3 cut(s) 367, 368, 1437
NdeII GATC 8 cut(s) 79, 288, 605, 690, 1187, 1239, 1258, 1563
NgoMIV GCCGGC 2 cut(s) 374, 1450
NlaIII CATG 3 cut(s) 275, 624, 1466
NlaIV GGNNCC 1 cut(s) 406
NmuCI GTSAC 2 cut(s) 382, 1387
PctI GAATGC 2 cut(s) 276, 484
PdiI GCCGGC 2 cut(s) 376, 1452
PfeI GAWTC 2 cut(s) 1458, 1488
PflMI CCANNNNNTGG 2 cut(s) 659, 1583
PkrI GCNGC 3 cut(s) 533, 565, 826
PleI GAGTC 3 cut(s) 150, 994, 1279
PpsI GAGTC 3 cut(s) 150, 994, 1279
PshBI ATTAAT 1 cut(s) 728
PsiI TTATAA 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 192
PspEI GGTNACC 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 401
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 406
PspPI GGNCC 5 cut(s) 405, 441, 453, 798, 1295
PstNI CAGNNNCTG 2 cut(s) 824, 854
PsuI RGATCY 1 cut(s) 1258
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 3 cut(s) 509, 893, 1478
RsaNI GTAC 3 cut(s) 508, 892, 1477
RseI CAYNNNNRTG 2 cut(s) 185, 1445
SaqAI TTAA 6 cut(s) 614, 728, 744, 1148, 1161, 1591
SatI GCNGC 3 cut(s) 532, 564, 825
Sau3AI GATC 8 cut(s) 79, 288, 605, 690, 1187, 1239, 1258, 1563
Sau96I GGNCC 5 cut(s) 405, 441, 453, 798, 1295
SchI GAGTC 3 cut(s) 150, 994, 1280
ScrFI CCNGG 4 cut(s) 194, 367, 368, 1437
SduI GDGCHC 2 cut(s) 366, 466
SfaNI GCATC 4 cut(s) 187, 654, 811, 1369
SfcI CTRYAG 1 cut(s) 805
SgrAI CRCCGGYG 1 cut(s) 374
SinI GGWCC 3 cut(s) 441, 453, 1295
SmaI CCCGGG 1 cut(s) 368
SmiMI CAYNNNNRTG 2 cut(s) 185, 1445
SmlI CTYRAG 5 cut(s) 115, 170, 430, 535, 1160
SmoI CTYRAG 5 cut(s) 115, 170, 430, 535, 1160
SpeI ACTAGT 2 cut(s) 668, 758
SsiI CCGC 1 cut(s) 531
SspMI CTAG 6 cut(s) 528, 669, 759, 1338, 1376, 1398
StyD4I CCNGG 4 cut(s) 192, 365, 366, 1435
StyI CCWWGG 5 cut(s) 139, 309, 493, 1191, 1383
TaaI ACNGT 4 cut(s) 658, 712, 814, 1335
TaiI ACGT 1 cut(s) 843
TaqI TCGA 4 cut(s) 673, 887, 1095, 1226
TatI WGTACW 1 cut(s) 891
TauI GCSGC 1 cut(s) 534
TfiI GAWTC 2 cut(s) 1458, 1488
Tru1I TTAA 6 cut(s) 614, 728, 744, 1148, 1161, 1591
Tru9I TTAA 6 cut(s) 614, 728, 744, 1148, 1161, 1591
TscAI CASTG 9 cut(s) 307, 547, 564, 585, 591, 1035, 1188, 1408, 1474
TseFI GTSAC 2 cut(s) 382, 1387
TseI GCWGC 2 cut(s) 563, 824
Tsp45I GTSAC 2 cut(s) 382, 1387
TspDTI ATGAA 3 cut(s) 50, 1125, 1260
TspGWI ACGGA 1 cut(s) 18
TspMI CCCGGG 1 cut(s) 366
TspRI CASTG 9 cut(s) 307, 547, 564, 585, 591, 1035, 1188, 1408, 1474
Van91I CCANNNNNTGG 2 cut(s) 659, 1583
Vha464I CTTAAG 1 cut(s) 1160
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 3 cut(s) 441, 453, 1295
VspI ATTAAT 1 cut(s) 728
XapI RAATTY 2 cut(s) 1005, 1321
XbaI TCTAGA 1 cut(s) 1337
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmaI CCCGGG 1 cut(s) 366
XmiI GTMKAC 1 cut(s) 1056
XspI CTAG 6 cut(s) 528, 669, 759, 1338, 1376, 1398
ZraI GACGTC 1 cut(s) 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.