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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 579 |
| AccII | CGCG | 1 cut(s) | 905 |
| AclWI | GGATC | 1 cut(s) | 1035 |
| AcoI | YGGCCR | 1 cut(s) | 305 |
| AcsI | RAATTY | 3 cut(s) | 212, 481, 842 |
| AcuI | CTGAAG | 3 cut(s) | 525, 1058, 1115 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 485, 777 |
| AgsI | TTSAA | 6 cut(s) | 272, 389, 817, 922, 1049, 1109 |
| AjnI | CCWGG | 2 cut(s) | 726, 945 |
| AluBI | AGCT | 4 cut(s) | 611, 796, 813, 1072 |
| AluI | AGCT | 4 cut(s) | 611, 796, 813, 1072 |
| AlwI | GGATC | 1 cut(s) | 1035 |
| AoxI | GGCC | 1 cut(s) | 305 |
| ApeKI | GCWGC | 2 cut(s) | 475, 653 |
| ApoI | RAATTY | 3 cut(s) | 212, 481, 842 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 966 |
| AspLEI | GCGC | 1 cut(s) | 907 |
| AspS9I | GGNCC | 1 cut(s) | 857 |
| AsuHPI | GGTGA | 3 cut(s) | 262, 647, 742 |
| AvaII | GGWCC | 1 cut(s) | 857 |
| BbsI | GAAGAC | 3 cut(s) | 11, 25, 459 |
| BbvCI | CCTCAGC | 1 cut(s) | 612 |
| BbvI | GCAGC | 2 cut(s) | 462, 640 |
| BccI | CCATC | 2 cut(s) | 241, 430 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 115, 149 |
| BciT130I | CCWGG | 2 cut(s) | 728, 947 |
| BclI | TGATCA | 1 cut(s) | 564 |
| BfaI | CTAG | 1 cut(s) | 972 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 882 |
| BisI | GCNGC | 2 cut(s) | 476, 654 |
| BlsI | GCNGC | 2 cut(s) | 477, 655 |
| Bme1390I | CCNGG | 2 cut(s) | 728, 947 |
| Bme18I | GGWCC | 1 cut(s) | 857 |
| BmgT120I | GGNCC | 1 cut(s) | 857 |
| BmiI | GGNNCC | 1 cut(s) | 933 |
| BmrFI | CCNGG | 2 cut(s) | 728, 947 |
| BmsI | GCATC | 1 cut(s) | 628 |
| BpiI | GAAGAC | 3 cut(s) | 11, 25, 459 |
| BpmI | CTGGAG | 2 cut(s) | 93, 737 |
| Bpu10I | CCTNAGC | 1 cut(s) | 612 |
| BsaBI | GATNNNNATC | 1 cut(s) | 440 |
| BsaJI | CCNNGG | 3 cut(s) | 726, 860, 897 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 485, 777 |
| Bse1I | ACTGG | 1 cut(s) | 751 |
| Bse3DI | GCAATG | 1 cut(s) | 648 |
| Bse8I | GATNNNNATC | 1 cut(s) | 440 |
| BseBI | CCWGG | 2 cut(s) | 728, 947 |
| BseDI | CCNNGG | 3 cut(s) | 726, 860, 897 |
| BseGI | GGATG | 3 cut(s) | 286, 381, 1168 |
| BseJI | GATNNNNATC | 1 cut(s) | 440 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 485, 777 |
| BseMI | GCAATG | 1 cut(s) | 648 |
| BseMII | CTCAG | 4 cut(s) | 30, 153, 603, 798 |
| BseNI | ACTGG | 1 cut(s) | 751 |
| BseRI | GAGGAG | 2 cut(s) | 19, 641 |
| BseXI | GCAGC | 2 cut(s) | 462, 640 |
| BsgI | GTGCAG | 2 cut(s) | 155, 207 |
| Bsh1236I | CGCG | 1 cut(s) | 905 |
| BshFI | GGCC | 1 cut(s) | 307 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 485, 777 |
| BsmI | GAATGC | 1 cut(s) | 1087 |
| BsnI | GGCC | 1 cut(s) | 307 |
| Bsp143I | GATC | 5 cut(s) | 321, 441, 564, 758, 1027 |
| Bsp19I | CCATGG | 1 cut(s) | 860 |
| BspANI | GGCC | 1 cut(s) | 307 |
| BspCNI | CTCAG | 4 cut(s) | 31, 154, 604, 799 |
| BspFNI | CGCG | 1 cut(s) | 905 |
| BspLI | GGNNCC | 1 cut(s) | 933 |
| BspPI | GGATC | 1 cut(s) | 1035 |
| BsrDI | GCAATG | 1 cut(s) | 648 |
| BsrI | ACTGG | 1 cut(s) | 751 |
| BssECI | CCNNGG | 3 cut(s) | 726, 860, 897 |
| BssMI | GATC | 5 cut(s) | 321, 441, 564, 758, 1027 |
| BssT1I | CCWWGG | 1 cut(s) | 860 |
| Bst2UI | CCWGG | 2 cut(s) | 728, 947 |
| Bst4CI | ACNGT | 1 cut(s) | 577 |
| Bst6I | CTCTTC | 1 cut(s) | 401 |
| BstDEI | CTNAG | 5 cut(s) | 39, 162, 612, 776, 807 |
| BstDSI | CCRYGG | 1 cut(s) | 860 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 775 |
| BstF5I | GGATG | 3 cut(s) | 286, 381, 1168 |
| BstFNI | CGCG | 1 cut(s) | 905 |
| BstHHI | GCGC | 1 cut(s) | 907 |
| BstKTI | GATC | 5 cut(s) | 324, 444, 567, 761, 1030 |
| BstMBI | GATC | 5 cut(s) | 321, 441, 564, 758, 1027 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 601, 693, 882 |
| BstNI | CCWGG | 2 cut(s) | 728, 947 |
| BstNSI | RCATGY | 1 cut(s) | 687 |
| BstSCI | CCNGG | 2 cut(s) | 726, 945 |
| BstUI | CGCG | 1 cut(s) | 905 |
| BstV1I | GCAGC | 2 cut(s) | 462, 640 |
| BstV2I | GAAGAC | 3 cut(s) | 11, 25, 459 |
| BstX2I | RGATCY | 1 cut(s) | 1027 |
| BstYI | RGATCY | 1 cut(s) | 1027 |
| BsuRI | GGCC | 1 cut(s) | 307 |
| BtgI | CCRYGG | 1 cut(s) | 860 |
| BtgZI | GCGATG | 1 cut(s) | 817 |
| BtsCI | GGATG | 3 cut(s) | 286, 381, 1168 |
| BtsI | GCAGTG | 1 cut(s) | 843 |
| BtsIMutI | CAGTG | 1 cut(s) | 843 |
| CfoI | GCGC | 1 cut(s) | 907 |
| Cfr13I | GGNCC | 1 cut(s) | 857 |
| CseI | GACGC | 1 cut(s) | 911 |
| CviAII | CATG | 3 cut(s) | 59, 684, 861 |
| DdeI | CTNAG | 5 cut(s) | 39, 162, 612, 776, 807 |
| DpnI | GATC | 5 cut(s) | 323, 443, 566, 760, 1029 |
| DpnII | GATC | 5 cut(s) | 321, 441, 564, 758, 1027 |
| EaeI | YGGCCR | 1 cut(s) | 305 |
| Eam1104I | CTCTTC | 1 cut(s) | 401 |
| EarI | CTCTTC | 1 cut(s) | 401 |
| Eco130I | CCWWGG | 1 cut(s) | 860 |
| Eco32I | GATATC | 2 cut(s) | 315, 988 |
| Eco47I | GGWCC | 1 cut(s) | 857 |
| Eco57I | CTGAAG | 3 cut(s) | 525, 1058, 1115 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 775 |
| EcoRI | GAATTC | 1 cut(s) | 842 |
| EcoRII | CCWGG | 2 cut(s) | 726, 945 |
| EcoRV | GATATC | 2 cut(s) | 315, 988 |
| EcoT14I | CCWWGG | 1 cut(s) | 860 |
| ErhI | CCWWGG | 1 cut(s) | 860 |
| FaeI | CATG | 3 cut(s) | 62, 687, 864 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 523, 555, 579, 611 |
| FatI | CATG | 3 cut(s) | 58, 683, 860 |
| FbaI | TGATCA | 1 cut(s) | 564 |
| FblI | GTMKAC | 1 cut(s) | 579 |
| Fnu4HI | GCNGC | 2 cut(s) | 476, 654 |
| FokI | GGATG | 3 cut(s) | 293, 368, 1175 |
| Fsp4HI | GCNGC | 2 cut(s) | 476, 654 |
| FspBI | CTAG | 1 cut(s) | 972 |
| GlaI | GCGC | 1 cut(s) | 906 |
| GluI | GCNGC | 2 cut(s) | 476, 654 |
| GsuI | CTGGAG | 2 cut(s) | 93, 737 |
| HaeIII | GGCC | 1 cut(s) | 307 |
| HgaI | GACGC | 1 cut(s) | 911 |
| HhaI | GCGC | 1 cut(s) | 907 |
| Hin1II | CATG | 3 cut(s) | 62, 687, 864 |
| Hin6I | GCGC | 1 cut(s) | 905 |
| HinP1I | GCGC | 1 cut(s) | 905 |
| HincII | GTYRAC | 1 cut(s) | 702 |
| HindII | GTYRAC | 1 cut(s) | 702 |
| HindIII | AAGCTT | 1 cut(s) | 1070 |
| HinfI | GANTC | 2 cut(s) | 645, 893 |
| HphI | GGTGA | 3 cut(s) | 262, 647, 742 |
| Hpy166II | GTNNAC | 3 cut(s) | 580, 678, 702 |
| Hpy188I | TCNGA | 5 cut(s) | 40, 163, 829, 892, 898 |
| Hpy188III | TCNNGA | 3 cut(s) | 486, 716, 1076 |
| Hpy8I | GTNNAC | 3 cut(s) | 580, 678, 702 |
| HpyAV | CCTTC | 4 cut(s) | 163, 395, 549, 625 |
| HpyCH4III | ACNGT | 1 cut(s) | 577 |
| HpyCH4IV | ACGT | 1 cut(s) | 1139 |
| HpyCH4V | TGCA | 7 cut(s) | 136, 188, 340, 641, 1085, 1103, 1121 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 601, 693, 882 |
| HpyF3I | CTNAG | 5 cut(s) | 39, 162, 612, 776, 807 |
| HpySE526I | ACGT | 1 cut(s) | 1139 |
| Hsp92II | CATG | 3 cut(s) | 62, 687, 864 |
| HspAI | GCGC | 1 cut(s) | 905 |
| Ksp22I | TGATCA | 1 cut(s) | 564 |
| Kzo9I | GATC | 5 cut(s) | 321, 441, 564, 758, 1027 |
| Lsp1109I | GCAGC | 2 cut(s) | 462, 640 |
| LweI | GCATC | 1 cut(s) | 628 |
| MaeI | CTAG | 1 cut(s) | 972 |
| MaeII | ACGT | 1 cut(s) | 1139 |
| MaeIII | GTNAC | 1 cut(s) | 368 |
| MalI | GATC | 5 cut(s) | 323, 443, 566, 760, 1029 |
| MboI | GATC | 5 cut(s) | 321, 441, 564, 758, 1027 |
| MfeI | CAATTG | 1 cut(s) | 1104 |
| MflI | RGATCY | 1 cut(s) | 1027 |
| MluCI | AATT | 7 cut(s) | 212, 226, 267, 481, 823, 842, 1104 |
| MmeI | TCCRAC | 2 cut(s) | 1122, 1137 |
| MroXI | GAANNNNTTC | 1 cut(s) | 966 |
| MseI | TTAA | 3 cut(s) | 243, 597, 1199 |
| MslI | CAYNNNNRTG | 2 cut(s) | 665, 836 |
| MspR9I | CCNGG | 2 cut(s) | 728, 947 |
| MunI | CAATTG | 1 cut(s) | 1104 |
| Mva1269I | GAATGC | 1 cut(s) | 1087 |
| MvaI | CCWGG | 2 cut(s) | 728, 947 |
| MvnI | CGCG | 1 cut(s) | 905 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 601, 693, 882 |
| NcoI | CCATGG | 1 cut(s) | 860 |
| NdeII | GATC | 5 cut(s) | 321, 441, 564, 758, 1027 |
| NlaIII | CATG | 3 cut(s) | 62, 687, 864 |
| NlaIV | GGNNCC | 1 cut(s) | 933 |
| NmuCI | GTSAC | 1 cut(s) | 368 |
| NspI | RCATGY | 1 cut(s) | 687 |
| PctI | GAATGC | 1 cut(s) | 1087 |
| PdmI | GAANNNNTTC | 1 cut(s) | 966 |
| PfeI | GAWTC | 2 cut(s) | 645, 893 |
| PkrI | GCNGC | 2 cut(s) | 477, 655 |
| Psp6I | CCWGG | 2 cut(s) | 726, 945 |
| PspGI | CCWGG | 2 cut(s) | 726, 945 |
| PspN4I | GGNNCC | 1 cut(s) | 933 |
| PspPI | GGNCC | 1 cut(s) | 857 |
| PsuI | RGATCY | 1 cut(s) | 1027 |
| RseI | CAYNNNNRTG | 2 cut(s) | 665, 836 |
| SaqAI | TTAA | 3 cut(s) | 243, 597, 1199 |
| SatI | GCNGC | 2 cut(s) | 476, 654 |
| Sau3AI | GATC | 5 cut(s) | 321, 441, 564, 758, 1027 |
| Sau96I | GGNCC | 1 cut(s) | 857 |
| ScrFI | CCNGG | 2 cut(s) | 728, 947 |
| SfaNI | GCATC | 1 cut(s) | 628 |
| SinI | GGWCC | 1 cut(s) | 857 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 665, 836 |
| Sse9I | AATT | 7 cut(s) | 212, 226, 267, 481, 823, 842, 1104 |
| SspMI | CTAG | 1 cut(s) | 972 |
| StyD4I | CCNGG | 2 cut(s) | 726, 945 |
| StyI | CCWWGG | 1 cut(s) | 860 |
| TaaI | ACNGT | 1 cut(s) | 577 |
| TaiI | ACGT | 1 cut(s) | 1142 |
| TaqI | TCGA | 5 cut(s) | 375, 761, 785, 1066, 1097 |
| TasI | AATT | 7 cut(s) | 212, 226, 267, 481, 823, 842, 1104 |
| TfiI | GAWTC | 2 cut(s) | 645, 893 |
| Tru1I | TTAA | 3 cut(s) | 243, 597, 1199 |
| Tru9I | TTAA | 3 cut(s) | 243, 597, 1199 |
| TscAI | CASTG | 1 cut(s) | 843 |
| TseFI | GTSAC | 1 cut(s) | 368 |
| TseI | GCWGC | 2 cut(s) | 475, 653 |
| Tsp45I | GTSAC | 1 cut(s) | 368 |
| TspDTI | ATGAA | 5 cut(s) | 17, 60, 75, 835, 882 |
| TspGWI | ACGGA | 1 cut(s) | 304 |
| TspRI | CASTG | 1 cut(s) | 843 |
| VpaK11BI | GGWCC | 1 cut(s) | 857 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 775 |
| XapI | RAATTY | 3 cut(s) | 212, 481, 842 |
| XceI | RCATGY | 1 cut(s) | 687 |
| XmiI | GTMKAC | 1 cut(s) | 579 |
| XmnI | GAANNNNTTC | 1 cut(s) | 966 |
| XspI | CTAG | 1 cut(s) | 972 |