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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 246 |
| Acc36I | ACCTGC | 1 cut(s) | 174 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 176 |
| AciI | CCGC | 1 cut(s) | 341 |
| AclWI | GGATC | 1 cut(s) | 320 |
| AcuI | CTGAAG | 2 cut(s) | 98, 364 |
| AcyI | GRCGYC | 1 cut(s) | 243 |
| AfaI | GTAC | 2 cut(s) | 83, 359 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 176 |
| AgsI | TTSAA | 2 cut(s) | 185, 367 |
| AluBI | AGCT | 3 cut(s) | 28, 43, 304 |
| AluI | AGCT | 3 cut(s) | 28, 43, 304 |
| Alw21I | GWGCWC | 2 cut(s) | 210, 306 |
| Alw26I | GTCTC | 1 cut(s) | 113 |
| AlwI | GGATC | 1 cut(s) | 320 |
| AoxI | GGCC | 1 cut(s) | 57 |
| AspS9I | GGNCC | 1 cut(s) | 190 |
| AvaII | GGWCC | 1 cut(s) | 190 |
| BanII | GRGCYC | 1 cut(s) | 306 |
| Bbv12I | GWGCWC | 2 cut(s) | 210, 306 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 286, 320 |
| BclI | TGATCA | 1 cut(s) | 3 |
| BcoDI | GTCTC | 1 cut(s) | 113 |
| BfuAI | ACCTGC | 1 cut(s) | 174 |
| BisI | GCNGC | 1 cut(s) | 342 |
| BlsI | GCNGC | 1 cut(s) | 343 |
| BmcAI | AGTACT | 1 cut(s) | 83 |
| Bme18I | GGWCC | 1 cut(s) | 190 |
| BmgT120I | GGNCC | 1 cut(s) | 190 |
| BmiI | GGNNCC | 1 cut(s) | 191 |
| BmsI | GCATC | 1 cut(s) | 236 |
| Bpu10I | CCTNAGC | 1 cut(s) | 194 |
| BpuEI | CTTGAG | 1 cut(s) | 194 |
| BsaHI | GRCGYC | 1 cut(s) | 243 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 176 |
| Bse1I | ACTGG | 2 cut(s) | 181, 285 |
| Bse3DI | GCAATG | 1 cut(s) | 137 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 176 |
| BseMI | GCAATG | 1 cut(s) | 137 |
| BseMII | CTCAG | 3 cut(s) | 53, 185, 307 |
| BseNI | ACTGG | 2 cut(s) | 181, 285 |
| BshFI | GGCC | 1 cut(s) | 59 |
| BsiHKAI | GWGCWC | 2 cut(s) | 210, 306 |
| BslFI | GGGAC | 1 cut(s) | 203 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 176 |
| BsmAI | GTCTC | 1 cut(s) | 113 |
| BsmFI | GGGAC | 1 cut(s) | 203 |
| BsmI | GAATGC | 1 cut(s) | 371 |
| BsnI | GGCC | 1 cut(s) | 59 |
| Bsp1286I | GDGCHC | 2 cut(s) | 210, 306 |
| Bsp143I | GATC | 2 cut(s) | 3, 325 |
| BspACI | CCGC | 1 cut(s) | 341 |
| BspANI | GGCC | 1 cut(s) | 59 |
| BspCNI | CTCAG | 3 cut(s) | 52, 186, 306 |
| BspLI | GGNNCC | 1 cut(s) | 191 |
| BspMI | ACCTGC | 1 cut(s) | 174 |
| BspPI | GGATC | 1 cut(s) | 320 |
| BsrDI | GCAATG | 1 cut(s) | 137 |
| BsrI | ACTGG | 2 cut(s) | 181, 285 |
| BssMI | GATC | 2 cut(s) | 3, 325 |
| BssNI | GRCGYC | 1 cut(s) | 243 |
| Bst4CI | ACNGT | 1 cut(s) | 265 |
| Bst6I | CTCTTC | 1 cut(s) | 368 |
| BstACI | GRCGYC | 1 cut(s) | 243 |
| BstDEI | CTNAG | 4 cut(s) | 39, 194, 293, 330 |
| BstKTI | GATC | 2 cut(s) | 6, 328 |
| BstMAI | GTCTC | 1 cut(s) | 113 |
| BstMBI | GATC | 2 cut(s) | 3, 325 |
| BsuRI | GGCC | 1 cut(s) | 59 |
| BtsI | GCAGTG | 1 cut(s) | 344 |
| BtsIMutI | CAGTG | 1 cut(s) | 344 |
| BveI | ACCTGC | 1 cut(s) | 174 |
| Cfr13I | GGNCC | 1 cut(s) | 190 |
| Csp6I | GTAC | 2 cut(s) | 82, 358 |
| CviAII | CATG | 3 cut(s) | 53, 250, 355 |
| CviJI | RGCY | 5 cut(s) | 28, 43, 59, 101, 304 |
| CviKI_1 | RGCY | 5 cut(s) | 28, 43, 59, 101, 304 |
| CviQI | GTAC | 2 cut(s) | 82, 358 |
| DdeI | CTNAG | 4 cut(s) | 39, 194, 293, 330 |
| DpnI | GATC | 2 cut(s) | 5, 327 |
| DpnII | GATC | 2 cut(s) | 3, 325 |
| Eam1104I | CTCTTC | 1 cut(s) | 368 |
| EarI | CTCTTC | 1 cut(s) | 368 |
| Ecl136II | GAGCTC | 1 cut(s) | 304 |
| Eco24I | GRGCYC | 1 cut(s) | 306 |
| Eco47I | GGWCC | 1 cut(s) | 190 |
| Eco53kI | GAGCTC | 1 cut(s) | 304 |
| Eco57I | CTGAAG | 2 cut(s) | 98, 364 |
| EcoICRI | GAGCTC | 1 cut(s) | 304 |
| EcoO109I | RGGNCCY | 1 cut(s) | 190 |
| EcoT22I | ATGCAT | 1 cut(s) | 373 |
| EcoT38I | GRGCYC | 1 cut(s) | 306 |
| FaeI | CATG | 3 cut(s) | 56, 253, 358 |
| FaiI | YATR | 6 cut(s) | 18, 54, 74, 149, 251, 356 |
| FaqI | GGGAC | 1 cut(s) | 203 |
| FatI | CATG | 3 cut(s) | 52, 249, 354 |
| FbaI | TGATCA | 1 cut(s) | 3 |
| Fnu4HI | GCNGC | 1 cut(s) | 342 |
| FriOI | GRGCYC | 1 cut(s) | 306 |
| Fsp4HI | GCNGC | 1 cut(s) | 342 |
| GluI | GCNGC | 1 cut(s) | 342 |
| HaeIII | GGCC | 1 cut(s) | 59 |
| Hin1I | GRCGYC | 1 cut(s) | 243 |
| Hin1II | CATG | 3 cut(s) | 56, 253, 358 |
| HincII | GTYRAC | 1 cut(s) | 247 |
| HindII | GTYRAC | 1 cut(s) | 247 |
| HindIII | AAGCTT | 1 cut(s) | 26 |
| Hpy166II | GTNNAC | 1 cut(s) | 247 |
| Hpy188I | TCNGA | 1 cut(s) | 8 |
| Hpy8I | GTNNAC | 1 cut(s) | 247 |
| HpyAV | CCTTC | 2 cut(s) | 196, 217 |
| HpyCH4III | ACNGT | 1 cut(s) | 265 |
| HpyCH4IV | ACGT | 1 cut(s) | 243 |
| HpyCH4V | TGCA | 2 cut(s) | 142, 371 |
| HpyF3I | CTNAG | 4 cut(s) | 39, 194, 293, 330 |
| HpySE526I | ACGT | 1 cut(s) | 243 |
| Hsp92I | GRCGYC | 1 cut(s) | 243 |
| Hsp92II | CATG | 3 cut(s) | 56, 253, 358 |
| Ksp22I | TGATCA | 1 cut(s) | 3 |
| Kzo9I | GATC | 2 cut(s) | 3, 325 |
| LmnI | GCTCC | 2 cut(s) | 48, 301 |
| LpnPI | CCDG | 7 cut(s) | 128, 162, 179, 206, 266, 288, 370 |
| LweI | GCATC | 1 cut(s) | 236 |
| MaeII | ACGT | 1 cut(s) | 243 |
| MalI | GATC | 2 cut(s) | 5, 327 |
| MboI | GATC | 2 cut(s) | 3, 325 |
| MboII | GAAGA | 6 cut(s) | 34, 222, 325, 355, 367, 370 |
| MhlI | GDGCHC | 2 cut(s) | 210, 306 |
| MluCI | AATT | 2 cut(s) | 155, 336 |
| MnlI | CCTC | 4 cut(s) | 49, 208, 246, 371 |
| Mph1103I | ATGCAT | 1 cut(s) | 373 |
| MseI | TTAA | 1 cut(s) | 93 |
| MslI | CAYNNNNRTG | 1 cut(s) | 51 |
| Mva1269I | GAATGC | 1 cut(s) | 371 |
| NdeII | GATC | 2 cut(s) | 3, 325 |
| NlaIII | CATG | 3 cut(s) | 56, 253, 358 |
| NlaIV | GGNNCC | 1 cut(s) | 191 |
| NmeAIII | GCCGAG | 1 cut(s) | 285 |
| NsiI | ATGCAT | 1 cut(s) | 373 |
| PctI | GAATGC | 1 cut(s) | 371 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 176 |
| PkrI | GCNGC | 1 cut(s) | 343 |
| PpuMI | RGGWCCY | 1 cut(s) | 190 |
| Psp124BI | GAGCTC | 1 cut(s) | 306 |
| Psp5II | RGGWCCY | 1 cut(s) | 190 |
| PspN4I | GGNNCC | 1 cut(s) | 191 |
| PspPI | GGNCC | 1 cut(s) | 190 |
| PspPPI | RGGWCCY | 1 cut(s) | 190 |
| RsaI | GTAC | 2 cut(s) | 83, 359 |
| RsaNI | GTAC | 2 cut(s) | 82, 358 |
| RseI | CAYNNNNRTG | 1 cut(s) | 51 |
| SacI | GAGCTC | 1 cut(s) | 306 |
| SaqAI | TTAA | 1 cut(s) | 93 |
| SatI | GCNGC | 1 cut(s) | 342 |
| Sau3AI | GATC | 2 cut(s) | 3, 325 |
| Sau96I | GGNCC | 1 cut(s) | 190 |
| ScaI | AGTACT | 1 cut(s) | 83 |
| SduI | GDGCHC | 2 cut(s) | 210, 306 |
| SetI | ASST | 9 cut(s) | 30, 45, 168, 195, 207, 246, 277, 306, 363 |
| SfaNI | GCATC | 1 cut(s) | 236 |
| SinI | GGWCC | 1 cut(s) | 190 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 51 |
| SmlI | CTYRAG | 1 cut(s) | 209 |
| SmoI | CTYRAG | 1 cut(s) | 209 |
| Sse9I | AATT | 2 cut(s) | 155, 336 |
| SsiI | CCGC | 1 cut(s) | 341 |
| SstI | GAGCTC | 1 cut(s) | 306 |
| TaaI | ACNGT | 1 cut(s) | 265 |
| TaiI | ACGT | 1 cut(s) | 246 |
| TaqI | TCGA | 1 cut(s) | 233 |
| TasI | AATT | 2 cut(s) | 155, 336 |
| TatI | WGTACW | 1 cut(s) | 81 |
| TauI | GCSGC | 1 cut(s) | 344 |
| Tru1I | TTAA | 1 cut(s) | 93 |
| Tru9I | TTAA | 1 cut(s) | 93 |
| TscAI | CASTG | 1 cut(s) | 351 |
| TspDTI | ATGAA | 1 cut(s) | 168 |
| TspGWI | ACGGA | 1 cut(s) | 235 |
| TspRI | CASTG | 1 cut(s) | 351 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 176 |
| VpaK11BI | GGWCC | 1 cut(s) | 190 |
| ZraI | GACGTC | 1 cut(s) | 244 |
| ZrmI | AGTACT | 1 cut(s) | 83 |
| Zsp2I | ATGCAT | 1 cut(s) | 373 |